Bioactive scaffold for inducing tendon tissue regeneration, preparation method and use thereof

A bioactive, tissue regeneration technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of difficulty in determining the appropriate concentration of each factor, limited types of bioactive factors, and unsatisfactory effects, to overcome mechanical Poor compatibility, good for cell adhesion, and high added value

Active Publication Date: 2018-07-06
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current method of biomodifying scaffolds to improve biological activity is usually to directly compound bioactive factors, but the types of bioactive factors in the composite scaffolds prepared by this method are limited, and the appropriate concentration of each factor is difficult to determine, so the biological activity is limited and the effect is limited. unsatisfactory

Method used

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  • Bioactive scaffold for inducing tendon tissue regeneration, preparation method and use thereof
  • Bioactive scaffold for inducing tendon tissue regeneration, preparation method and use thereof
  • Bioactive scaffold for inducing tendon tissue regeneration, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Bioactive Scaffold for Inducing Tendon Tissue Regeneration

[0069] Using longitudinal slices of canine hindlimb Achilles tendon as the base material, bioactive scaffolds were prepared by modifying tendon slices with extracellular matrix of tendon stem cells. The specific preparation method was as follows:

[0070] Preparation of slice scaffolds: 1) take fresh tendon and wash it; 2) freeze and thaw the tendon repeatedly; the repeated freezing and thawing is to place the tendon cleaned in step 1) in liquid nitrogen for 1 to 3 minutes, and place it at 25 to 37°C for 3 minutes. ~10min, repeat the operation 4-6 times; 3) Frozen section the tendon after the freeze-thaw treatment in step 2) along the length direction, and the slice thickness is 300-900 μm; 4) Treat the tendon slice obtained in step 3) with nuclease , cleaning, and get final product; Wherein, the method for nuclease treatment is: take the tendon section that step 3) obtains, place the nuclease soluti...

Embodiment 2

[0073] Example 2 Bioactive Scaffold Inducing Tendon Tissue Regeneration

[0074] The calf Achilles tendon pellet was used as the base material, and the tendon pellet was modified with the extracellular matrix of tendon stem cells to prepare a bioactive scaffold. The specific preparation method was as follows:

[0075] Preparation of tablet support: It can be prepared according to the method of claim 1 of patent No. ZL201310636964.0, specifically, 1) take fresh tendons and clean them; 2) compress the tendons along the thickness direction, and the compression rate is 60-90 %, compressed into a tendon tablet with a thickness of about 1.0 to 1.2 mm; 3) repeatedly freezing and thawing the compressed tendon in step 2); the repeated freezing and thawing is placing the compressed tendon in step 2) in liquid nitrogen for 1 ~3min, place at 25~37°C for 3~10min, repeat the operation 4~6 times; 4) treat the tendon treated in step 3) with nuclease, and wash it; wherein, the method of nuclea...

Embodiment 3

[0078] Example 3 Bioactive Scaffold Inducing Tendon Tissue Regeneration

[0079] 1. Preparation method

[0080] The calf Achilles tendon tablet was used as the base material to prepare the tendon gel and tablet scaffold composite, which was used to repair the Achilles tendon defect and reconstruct the function of the Achilles tendon in white rabbits. The specific preparation and use methods were as follows:

[0081]Preparation of tablet support: It can be prepared according to the method of claim 1 of patent No. ZL201310636964.0, specifically, 1) take fresh tendons and clean them; 2) compress the tendons along the thickness direction, and the compression rate is 60-90 %, compressed into a tendon tablet with a thickness of about 1.0 to 1.2 mm; 3) repeatedly freezing and thawing the compressed tendon in step 2); the repeated freezing and thawing is placing the compressed tendon in step 2) in liquid nitrogen for 1 ~3min, place at 25~37°C for 3~10min, repeat the operation 4~6 tim...

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Abstract

The invention discloses a method for preparation of a bioactive scaffold for inducing tendon tissue regeneration. The method includes the steps of: (1) taking a fresh tendon and conducting decellularization treatment to obtain a decellularized compressed sheet or slice scaffold, and (2) compounding an extracellular matrix on the scaffold. The invention also discloses a decellularized tendon bioactive scaffold prepared by the aforementioned method and use thereof. The decellularized tendon scaffold prepared by the method provided by the invention has the characteristics of high bioactivity, good in-vivo restoration effect, low immunogenicity, good biocompatibility, simple preparation method and low cost, thus having good application prospects.

Description

technical field [0001] The invention relates to a preparation method and application of a bioactive scaffold capable of regeneration of tendon tissue, belonging to the field of biomaterials. Background technique [0002] Tendon injuries caused by diseases, trauma, degeneration and other reasons have become a health problem that cannot be ignored. At present, there are various methods for repairing tendon injuries, including autologous tendon transplantation, allogeneic or heterogeneous tendon transplantation, and artificial material replacement. Autologous tendon transplantation will cause pain, infection, and injury at the donor site, and at the same time, the source of tendon supply is limited; allogeneic tendon transplantation may cause the spread of diseases, and there are different degrees of rejection, and there are also problems with limited sources; xenogeneic tendon transplantation It may also cause the spread of diseases, but the main problem is the existence of i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/54A61L27/58
CPCA61L27/3604A61L27/3633A61L27/3662A61L27/3687A61L27/3691A61L27/50A61L27/54A61L27/58A61L2300/252A61L2300/414A61L2430/10A61L2430/40A61L27/3834A61L27/386A61L2300/64A61L27/3895
Inventor 秦廷武宁良菊张亚靖崔静姚璇罗静聪
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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