543 results about "Freeze and thaw" patented technology

The invention provides a sterile polymerized covering for a wound surface-medical wet dressing for a burn wound, a preparation method of the dressing, and application. The dressing comprises the following components: 10 to 30g of chitosan, 30 to 80g of polyving akohol, 5 to 10g of alginate, 5 to 15g of isomaltose hypgather, 2 to 6g of carbomer 940, 2 to 5g of gelatin, 5 to 10g of sodium carboxymethylcellulose, 10 to 30g of glycerol, and 0.01 to 0.2g of menthol; 15 to 60g of condensed tannin which is remarkable in treatment can be selectively added; and a skin penetration enhancer or an appearance conditioning agent also can be selectively added. The dressing can be prepared into an irregular dressing in order to be suitable for the wound surfaces in irregular and special positions and also can be prepared into a solid plate-shaped dressing which can be shorn at random. According to the sterile polymerized covering dressing for the wound surface, the freezing and thawing technology and the irradiating technology are combined and cross-linked to realize the preparation, and the defects of the prior art can be overcome. The dressing is high in biocompatibility, free from toxicity and sensitization and high in mechanical strength and comfort level, is out of adhering during the process of being replaced after being attached for a long time, and is a nonantigenic protective barrier which has the effect of resisting and inhibiting bacteria.

The invention discloses a technique for extracting phycocyanin, chlorophyll and spirulina polysaccharide in spirulina, comprising the following steps: taking and weighing fresh spirulina, centrifuging and washing the spirulina, weighing spirulina clay, adding water into the supernatant, stirring evenly, and freezing and thawing twice to break the cell wall, after the second dissolving, adding water, placing for 2-8 hours, centrifuging to obtain supernatant and sediment A, extracting the sediment A by a Soxhlet extracted method with ethanol to divide the sediment A into extraction and sediment B, wherein the supernatant is used for extracting phycocyanin, the sediment A is used for extracting sodium zinc chlorophyllin and the sediment B is used for extracting spirulina polysaccharide. The extraction technique extracts the active substances such as phycocyanin, chlorophyll and spirulina polysaccharide, and fully and effectively utilizes the prior spirulina resource and avoids the resource waste.