Technique for extracting phycocyanin, chlorophyll and spirulina polysaccharide in spirulina

A technology of spirulina polysaccharide and phycocyanin, which is applied to the preparation method of peptides, algae/moss peptides, chemical instruments and methods, etc., can solve the waste of resources, cannot fully and effectively utilize spirulina resources, and cannot take into account a variety of biological Active substances and other issues to achieve the effect of avoiding waste

Inactive Publication Date: 2009-12-23
威海格瑞安生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology extracts different types of compounds from plants called spiririns that are found throughout nature or used for various purposes like medicine production. It can be useful because it uses all available sources without wasting any material at once.

Problems solved by technology

This patented describes how spirolakoid plants called Heliconia have been found that contain various types of proteins such as albumin, carbohydrate, beta -Caritenes, gamma linolefies, fats, etc., along with many different compounds needed for good physical activity like energy production from photosynthesis. These materials could potentially provide valuable medicines and products useful for use in industrial applications due their unique properties including being able to absorb light efficiently when exposed to sunlight without losing any coloring matter during storage periods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Extraction of phycocyanin, chlorophyll and spirulina polysaccharide from spirulina, the steps are as follows:

[0015] 1) Take 6kg of fresh spirulina, centrifuge and wash once, weigh the algae mud weight to 2kg, add 2L of water to the supernatant, stir well, freeze and thaw twice (freeze at 20℃ overnight, dissolve at room temperature), after the second dissolution , Then add 6L of water, leave it for 3h, centrifuge, get the supernatant and precipitate A.

[0016] 2) Take the above supernatant, adjust the pH to 4.2-4.5 with 6mol / L hydrochloric acid, stir for half an hour and then centrifuge (4000rpm, 15min). The supernatant is ultrafiltered once with an ultrafiltration membrane with a molecular weight of 6k, and then 4 times distilled water is added for ultrafiltration. Filter once, adjust the pH to 7.0 and freeze-dry to obtain phycocyanin powder.

[0017] 3) Precipitate A was extracted with ethanol Soxhlet for 8 hours (90% ethanol, excess zinc sulfate, hydrochlori...

Embodiment 2

[0019] Example 2 Extraction of phycocyanin, chlorophyll and spirulina polysaccharide from spirulina, the steps are as follows:

[0020] 1) Take 10kg of fresh spirulina, centrifuge and wash once, weigh the algae mud weight to 4kg, add 4L water, stir evenly, freeze and thaw twice (freeze at 20℃ overnight, dissolve at room temperature), add after the second dissolution Place 12L of water for 3h and centrifuge to get the supernatant and precipitation A.

[0021] 2) Take the above supernatant, adjust the pH to 4.2-4.5 with 5mol / L hydrochloric acid, stir for half an hour, and centrifuge (4000rpm, 15min). The supernatant is ultrafiltered once with an ultrafiltration membrane with a molecular weight of 6k, and then 4 times distilled water is added for ultrafiltration. Filter once, adjust pH to 7.0 and freeze-dry to obtain phycocyanin powder.

[0022] 3) Precipitate A was Soxhlet extracted with ethanol for 10 hours (90% ethanol, excess zinc sulfate, hydrochloric acid to adjust pH 2~3), the...

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PUM

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Abstract

The invention discloses a technique for extracting phycocyanin, chlorophyll and spirulina polysaccharide in spirulina, comprising the following steps: taking and weighing fresh spirulina, centrifuging and washing the spirulina, weighing spirulina clay, adding water into the supernatant, stirring evenly, and freezing and thawing twice to break the cell wall, after the second dissolving, adding water, placing for 2-8 hours, centrifuging to obtain supernatant and sediment A, extracting the sediment A by a Soxhlet extracted method with ethanol to divide the sediment A into extraction and sediment B, wherein the supernatant is used for extracting phycocyanin, the sediment A is used for extracting sodium zinc chlorophyllin and the sediment B is used for extracting spirulina polysaccharide. The extraction technique extracts the active substances such as phycocyanin, chlorophyll and spirulina polysaccharide, and fully and effectively utilizes the prior spirulina resource and avoids the resource waste.

Description

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Claims

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Application Information

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Owner 威海格瑞安生物工程有限公司
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