385 results about "Spirodela" patented technology

The invention discloses a technique for extracting phycocyanin, chlorophyll and spirulina polysaccharide in spirulina, comprising the following steps: taking and weighing fresh spirulina, centrifuging and washing the spirulina, weighing spirulina clay, adding water into the supernatant, stirring evenly, and freezing and thawing twice to break the cell wall, after the second dissolving, adding water, placing for 2-8 hours, centrifuging to obtain supernatant and sediment A, extracting the sediment A by a Soxhlet extracted method with ethanol to divide the sediment A into extraction and sediment B, wherein the supernatant is used for extracting phycocyanin, the sediment A is used for extracting sodium zinc chlorophyllin and the sediment B is used for extracting spirulina polysaccharide. The extraction technique extracts the active substances such as phycocyanin, chlorophyll and spirulina polysaccharide, and fully and effectively utilizes the prior spirulina resource and avoids the resource waste.

The invention provides five-alga probiotic enzyme tablets and a preparation method thereof. The five-alga probiotic enzyme tablets are prepared from the following raw materials in parts by mass: 10-20 parts of chlorella vulgaris, 1-10 parts of dunaliella salina, 1-10 parts of euglena, 1-10 parts of red algae, 10-20 parts of spirulina and 10-20 parts of probiotic powder. The five-alga probiotic enzyme tablets provided by the invention adopt the chlorella, dunaliella salina, red algae, spirulina and euglena for complementation in nutrition, and obtain trace elements easier to absorb by a human body through probiotic fermentation, thereby helping to reinforce the immunologic function of the human body, resist virus infection and proliferation, inhibit cancer cell proliferation, repair the damage of an organism quickly, remove vivotoxin, suppress blood pressure and blood sugar rise, reduce the serum cholesterol content, and thus improve the comprehensive conditioning of the human body.

A disclosed soil amendment is prepared from the following raw materials in parts by weight: 50-80 parts of water, 5-10 parts of spirulina, 30-50 parts of an organic fertilizer, 20-30 parts of peat soil, 10-18 parts of zinc sulfate , 10-15 parts of acrylamide, 8-16 parts of gypsum, 10-15 parts of sodium acrylate, 25-35 parts of montmorillonite, 11-21 parts of , 20-28 parts of furnace ash, 12-18 parts of blast-furnace slag, 7-14 parts of calcium magnesium phosphate, 4-8 parts of borax and 15-20 parts of pig manure. The provided soil amendment is capable of promoting growth of crops, enhancing resistance of crops, improving the soil structure of sand, increasing basic nutrients, stimulating growth of crops, and improving capability of resisting diseases, resisting drought and resisting cold.

The invention discloses a method for extracting polysaccharide of spirulina platensis. The method comprises the steps that spirulina platensis is subjected to an extraction process to form an extracting solution, the extracting solution is filtered through a microfiltration membrane and is subjected to ultrafilteration, concentration and drying, then more than one type of crude polysaccharide of aqueous extract spirulina platensis is obtained, the content of total sugar is larger than or equal to 10%, and the molecular weight is more than 5,000; or the extracting solution is concentrated and decontaminated, a processed solution is centrifuged under the rotating speed lower than or equal to 15,000 r/min, filtered through the microfiltration membrane and subjected to ultrafilteration by a filter material with the molecular weight in a range from 5,000 to 200,000, when no effluent flows out from an outlet of an ultrafilter, the effluent of the ultrafilter and a concentration solution in the ultrafilter are collected to be dried, then more than one type of polysaccharide of aqueous extract spirulina platensis is fromed, the content of total sugar is larger than or equal to 40%, and the molecular weight is in a range from 5,000 to 200,000. According to the method for extracting polysaccharide of spirulina platensis, reasonable processing measures are selected at different stages of a proces route according to different requirements for product purity, and then high purity olysaccharide with the scheduled molecular weight specification can be formed without detection; and moreover, polysaccharide of spirulina platensis with a pluraity of specifications can be formed simultaneosuly. Accordingly, the method has the advanatags which are impossible to be obtained by other traditional methods.

The invention relates to an active spirulina polysaccharide and a preparation method thereof. By introducing an activity evaluation index, discussing the influence of a raw material pretreatment method and different extraction processing technologies on spirulina polysaccharide yield, polysaccharide preservation rate, protein removal rate and polysaccharide lipid peroxidation resistance and evaluating and screening an extraction processing technology capable of well keeping the bioactivity of the spirulina polysaccharide and with relatively high spirulina polysaccharide yield, the active spirulina polysaccharide is prepared by preferable process conditions; the main active ingredients of the active spirulina polysaccharide are binding protein polysaccharides, the molecular weights of which are 100,000 and 95,000 respectively; the active spirulina polysaccharide has remarkable hepatic tissue lipid peroxidation activity resistance; and the two binding protein polysaccharides are qualitycontrol index ingredients of a spirulina lipid peroxidation activity resistant polysaccharide product. Compared with a method discussed in the conventional technology, the protection of the extraction, separation and purification processes on the activity of the spirulina polysaccharide is stressed; and by comprehensively evaluating and optimizing the preparation process conditions, blindness of the process discuss and destroy and loss of the active ingredients in the extraction and purification processes can be greatly reduced.

The invention relates to glucose-rich spirulina polysaccharide and an application thereof in a plant green pesticide and an anti-tumor drug. High-sugar spirulina obtained through nutritional regulation is used as a raw material and is subjected to hot water extraction, protein removal with a flocculant, concentration, precipitation and freeze drying to obtain the water-soluble high-sugar spirulina polysaccharide having the molecular weight of 10-200 kDa; the spirulina polysaccharide comprises the monosaccharides mainly comprising rhamnose, fucose, arabinose, xylose, mannose, galactose, glucose, glucuronic acid and galacturonic acid, and is characterized in that the glucose content is more than 50% in the monosaccharide composition. The spirulina polysaccharide can obviously induce plant disease resistance, has obvious anti-tumor effect on tumor, and can be used as the biological pesticide and the anti-tumor drug.

The invention relates to a spirulina phatensis polysaccharide and an extraction method thereof. The extraction method comprises the following steps: after multigelation and wall breaking of a spirulina phatensis powder suspension, centrifuging to obtain a cell lysate 1 and cell debris; adding ammonium sulfate in the cell lysate 1 until the saturation is 50%, after salting-out precipitation, centrifuging to remove phycobiliprotein to obtain a supernatant, and evenly mixing the supernatant and the cell debris to obtain a cell lysate 2; obtaining a spirulina phatensis polysaccharide crude extract by using a hot water extraction method; adding the crude extract to an ethanol/ammonium sulfate aqueous two-phase system, and extracting to obtain a bottom-phase solution of spirulina phatensis polysaccharide with higher purity; after the bottom-phase solution is desalted by dialysis, eluting by using a Sephadex G-150 chromatographic column and a DEAE Sephadex A-50 anion exchange column to obtain a pure spirulina phatensis polysaccharide solution; and freeze-drying, thereby obtaining a pure spirulina phatensis polysaccharide finished product. The extraction method can be used for avoiding the traditional complicated protein removal operation steps, has low cost, high yield, and high purity and activity of polysaccharide, and is suitable for intermittent and large-scale production and processing.

The invention provides a method for extracting phycobiliprotein from spirulina. The method comprises the following steps: adding water into spirulina mud to obtain a spirulina cell suspension, performing ultrasonic treatment to obtain spirulina lysate, centrifuging the lysate and taking the lysate, sequentially filtering through filter membranes which are respectively 0.8mu m and 0.45mu m, to obtain phycocyanin crude extracting liquid, then sequentially ultrafiltrating the crude extracting liquid through an ultrafiltration membranes with molecular weight cut-off of 300KD and 100KD, collecting a filtrate of the molecular section, and performing freezing and drying to the filtrate to obtain phycobiliprotein powder. According to the method, the defect of using chemical reagent to extract phycobiliprotein in the prior art is overcome, no residuals exist in the preparation, and the environment pollution does not exist. The extraction rate of the phycobiliprotein is greater than 80%, the content of phycobiliprotein is more than 60%, and the purity of the phycobiliprotein is more than 1.5. The overall technological process is safe and reliable, fast and efficient. Compared with the traditional method, by using the method, the content of the phycobiliprotein is high before using of chromatography, the phycobiliprotein with the purity not only can meet the requirement of food and cosmetic industries, but also can be applied to aspects of medical and health products, and the like.

The invention relates to a germ feed for fancy carps. The germ feed comprises the following components in parts by weight: 75-85 parts of fish meal, 45-55 parts of wheat germ powder, 20-40 parts of lucerne meal, 15-25 parts of phospholipid oil, 35-45 parts of corns, 45-55 parts of beer yeasts, 170-230 parts of bean pulp, 15-25 parts of seaweed meal, 1.8-2.2 parts of methionine, 80-120 parts of spiral seaweed, 8-13 parts of grain powder, 45-55 parts of expanding soybeans, 280-330 parts of flour, 8-13 parts of shrimp meal, and premixed compounds. The germ feed for fancy carps provided by the invention is used together with various raw materials; the reasonable proportion of the nutritional ingredients, such as crude fiber, unsaturated fatty acid, vitamin, mineral substance, amino acid, and the like, is achieved; and the feed with low cost and capacity of causing the fancy carps to grow quickly is obtained.

The invention refers to a making method of algae blue protein by using seawater spirulina as raw material. The current making method has long technical course, long cycle of extraction, high cost, low efficiency, low application value and mostly is made at low temperature. The invention can largely and at low cost extract higher-purity algae glue protein, is applied to research and development of natural pigment, health food and new sea medicament.

The invention discloses an aquarium fish feed comprising the following raw materials: by weight, 20-23 parts of fishmeal, 12-15 parts of housefly larvae powder, 30-35 parts of corn starch, 10-12 parts of flour, 40-45 parts of soybean meal, 3-4 parts of beer yeast powder, 3-4 parts of spirulina, 4-5 parts of hawthorn, 1-2 parts of citric acid, 1-2 parts of fumaric acid, 4-5 parts of diatomite powder, 2-3 parts of phtheirospermum tenuisectum Bur. et Franch, 1-2 parts of catalpa flower and 4-5 parts of a feeding promoting agent; the aquarium fish feed is prepared from a plurality of materials, is rich in proteins and multiple vitamins, is reasonable in the ratio of every nutrition ingredient, can provide comprehensive nutrition for aquarium fishes, at the same time, enhances the immune power of the aquarium fishes by adding of a plurality of Chinese herbal medicines, and reduces mortality.

The invention discloses a composite culture medium for fermentation of bifidobacteria and the manufacturing method, which comprises the following parts: 50-150g spirulina powder, 40-80g stachyose, 30-100ml 5% pork liver infusion, 1000ml distilled water. The effect of the invention is that: the infant bifidobacteria, adolescent bifidobacteria and long bifidobacteria grow well in the culture medium; the maximum increment reaches 1010cfu/ml in the best fermentation condition; the submerged culture effect is the best of the classic bifidobacteria selective culture medium, TPY culture medium and improved TPY culture medium. The composite culture medium provides a simple formulation, a low cost, a simple manufacturing process, which can be used to the research, exploitation, production of bifidobacteria, provides the important meanings of utilizing and developing spirulina and stachyose resource.

The invention discloses a breeding method of Spirulina alga species, which comprises the following steps: 1. selecting 5L of alga culture solution, filtering with a silk fabric to remove smaller algae; 2. carrying out mutagenesis with a phosphate buffer solution; 3. selecting single Spirulina with larger body and more spiral rings; culturing in a test tube, and calculating the growth rate; 4. culturing the Spirulina elements subjected to amplification culture in a 40001*12 DEG C lighting incubator; and 5. selecting the Spirulina element with the highest growth rate to obtain the mutant line subjected to mutagenesis screening, purification and cloning. The method changes the coarse breeding method of screening the alga species by the filter screen into capillary selected breeding; and the unicell screening is utilized to remove bad Spirulina individuals to obtain high purification, and the mutagenesis passivation is performed to obtain the high-quality high-growth-rate Spirulina alga species. The alga species purification and culture conditions are continuously optimized, so that the Spirulina yield is enhanced to 30-50%, and the product quality is further improved.

A soft-shelled turtle feed containing egg yolk and fish oil comprises the following raw materials in parts by weight: ginger oil 0.1-0.2, spirulina 1-2, oat flour 30-40, soy bean protein powder 10-20, egg yolk powder 6-8, chestnut 5-8, orange peel 2-4, Ligusticum chuanxiong Hort 1-2, flos sophorae immaturus 1-2, brown rice 5-15, kalimeris indica 8-10, amaranth leaves 4-6, salicornia europaea 4-6, clover 3-5, lecithin 1-2 and fish oil 1-2. The soft-shelled turtle feed contains health raw materials such as kalimeris indica, salicornia europaea and clover, and moreover the salicornia europaea has the oxidation resistance, anti-inflammation and immune regulation effect, and the health growth of soft-shelled turtle can be promoted.

The invention provides a method for culturing spirulina by using livestock and poultry excrement wastewater, belonging to the fields of sewage treatment and algal culture. The method comprises the following steps: (1) pretreatment of livestock and poultry excrement wastewater: filtering, and sterilizing the filtrate to obtain a culture solution; (2) domestication of spirulina: inoculating an alga strain into fresh water or sea water, and performing domestication culture; (3) culture of spirulina: inoculating the spirulina in a logarithmic growth phase of the step (2) into the culture solution of the step (1), and culturing; (4) collection of spirulina: filtering the spirulina cultured in the step (3), cleaning, collecting the spirulina, and then drying the spirulina; and (5) filtrate recovery. The method provided by the invention solves the problems that the livestock and poultry excrement pollutes the environment and the spirulina culture cost is high. The method can realize the triple effects of protecting the environment, lowering the spirulina culture cost and producing nutrient substances required by human beings.

The invention discloses a seawater spirulina selenium-rich culture method. The method comprises the following steps of: selecting one or combination of more of the seawater-naturalized spirulina platensis, spirulina maxima and microcystis aeruginos as the algae seed; based on the natural seawater, artificial seawater or the mixture of the two at any ratio, adding a selenium element with concentration of 1-160mg/L to serve as a culture medium; inoculating the algae seed into a photobioreactor containing the culture medium for culture; and then collecting the cultured spirulina body to obtain the seawater spirulina which is the selenium-rich seawater spirulina. Through the invention, the method is simple, the selenium addition concentration is low, the culture medium can be recycled for a long time, the operation is simple and convenient, the selenium enrichment ability is strong, the cost is low, the method is green and safe, etc. The bioactive substance of the seawater spirulina generates a synergistic effect with organic selenium in the algae body to realize the function, and can be applied to various diseases caused by selenium lack and oxidative damage of oxygen free radical for the selenium supplement additive, oxidation resistance, aging resistance, tumor resistance, Keshan disease prevention and treatment and the like.

The invention discloses a feeding method of reducing the disease and insect pest occurrence of stichopus japonicus. The method comprises the steps of building a stichopus japonicus feeding pond and a substratum; building the pond suitable for the growth of the stichopus japonicus and throwing stichopus japonicus seedlings into the pond by combining with the actual conditions according to the biological habit requirements of the stichopus japonicus; under the general condition, the state that big seedlings of over 600 heads/kg are thrown in the pond in a specification of 10 heads/m<2> to 20 heads/m<2> is proper, and if the specification is larger, the throwing amount in unit area can be smaller; the feeding feed formula comprises 20-25 parts of animal protein hydrolysate dry powder, 15-20 parts of meat and bone meal, 20-22 parts of soybean meal, 4-6 parts of honeysuckle, 2-3 parts of medicated leaven, 3-5 parts of angelica sinensis, 2-3 parts of soya bean lecithin, 1-2 parts of alginate-derived oligosaccharide, 1-2 parts of vitamin C, 1-2 parts of spirulina, 2-3 parts of lupulus, 3-5 parts of tea saponin, 2-3 parts of fructus cnidii, 3-4 parts of fructus ulmi, 1-2 parts of golden larch bark. The management of the stichopus japonicus seedlings is that when winter comes, the water depth of the pond is properly increased, and the change of dissolved oxygen and substrate and the prevention and control of seaweeds must be paid attention to; when the water temperature of the pond rises at spring, the transparency of the water is reduced by fertilizing the water, and the breeding of the seaweeds is controlled.

The invention discloses an extraction and determination method of crude polysaccharide in ganoderma spirulina, which comprises the following steps: weighing ganoderma spirulina samples accurately and putting the samples into a great capacity centrifuge tube, adding ethanol aqueous solution and mixing evenly, carrying out ultrasonic extraction and centrifugation, and discarding solution; adding the ethanol aqueous solution to the obtained sediment, vibrating and mixing evenly, carrying out ultrasonic extraction and centrifugation, precipitating, then moving to a round bottomed flask after adding water, after extraction in boiling water bath, filtering, bringing filtrate to volume, and determining the content of the crude polysaccharide by phenol-sulphuric acid method by taking glucose or dextran standardized products solution as a reference material. The method has simple steps, is accurate, has high sensitivity and is especially appropriate for the extraction and determination of the crude polysaccharide in the ganoderma spirulina which is health food and does not contain disruptors, dextrin, starch and the like.

The invention relates to an extraction method of water-soluble spirulina phycobiliprotein. The extraction method of the water-soluble spirulina phycobiliprotein comprises the following steps: soaking a spirulina raw material into water, stirring and freezing the solution at a temperature of 20 DEG C below zero for 48 hours, taking out and rapidly melting the frozen matter, centrifugating and filtering the solution, extracting the phycobiliprotein from the centrifugal liquid and remaining the spirulina dreg for extracting spirulina polysaccharide; and purifying, concentrating, spraying and drying the obtained centrifugal liquid into the spirulina phycobiliprotein. The extraction method of the water-soluble spirulina phycobiliprotein is simple to operate; the extracted spirulina phycobiliprotein is water-soluble powder so as to be easy for human bodies to desist and absorb, and the water-soluble powder can be further processed to be used as a raw material of food additives, functional foods and medicaments, a natural blue pigment of foods and cosmetics, and the like, thereby having wide serviceable range; and the pretreated spirulina dreg in the extraction process can be remained for extracting the spirulina polysaccharide so as to improve the utilization rate of the spirulina raw material.

The invention refers to a method for reconstructing the cloned and constructed human body tumor death factor TRAIL gene expression carrier into spirulina gene group to be expressed stably with gene gun. The special components of the reconstructed TRAIL expression carrier include resistant mark gene expression box CAT, chlorophyll body photosynthetic gene promotor and ender, spirulina homologous segment, TRAIL gene segment without coding film combining part, the expression system also includes characters of primary nucleus and eukarya. The invention sets suitable striking parameter, uses gene gun to guide the reconstructed expression carrier into spirulina silk body, and through several times of sifting of chloramphenicol with incremental density and molecular biology testing, the invention gets spirulina converter of TRAIL gene. The invention applies to spirulina reconstruction and human body gene expression, it also applies to reconstruct and express other primary nucleolus and eukarya.

The treatment of spirulina by a process which includes placing spirulina that has not been previously heat-sterilized, lactic acid bacteria and sugar in water, then culturing the lactic acid bacteria provides treated spirulina in which the distinctive taste and odor of spirulina are minimized, in which active ingredients such as phycocyanin remain intact, and which contains a reduced level of bacteria other than lactic acid bacteria.

A full-nutritive wheat flour contains wheat flour (83-93 wt portions), soybean powder (4-8), millet flour (3-5), sweet potato starch (0.5-1.5), lotus root starch (0.1-0.3), walnut powder (0.3-0.7) and spirulina (0.25-0.35). Its advantages include rich nutrients and trace elements, good enjoyment to eat it, good appearance and balanced nutrition to improve resistance to diseases.

The invention discloses a maca dunaliella salina compound preparation. The maca dunaliella salina compound preparation comprises dunaliella salina and spirulina and is prepared from the following materials: 3-10 percent of dunaliella salina, 40-45 percent of spirulina and 45-55 percent of maca. The maca dunaliella salina compound preparation has the effects of building body and tonifying Yang, curing organs, maintaining beauty and keeping young and improving the digestive system, achieves a two-way blood-pressure regulating function, as well as a two-way blood sugar regulating and balancing function, can regulate body metabolism and resist aging, tumor and fatigue and enhance the immunity, and has remarkable effects on cardio-cerebrovascular diseases, chronic diseases, senile dementia, climacteric syndrome and the like. When drunk frequently, the maca dunaliella salina compound preparation increases sexual intercourse, keep youth for a long time and prolongs the lifetime, has the advantages of relaxing tendons and activating collaterals, activating blood circulation to dissipate blood stasis, dispelling wind to eliminate dampness and strengthening bones and tendons and can remarkably improve memory.

The invention relates to the production of healthcare port wine in the process of food processing, which particularly relates to a spirulina port wine and the preparing technique, wherein the spirulina port wine is mixed by spirulina primary liquid and port wine stock solution, wherein the adding amount of spirulina primary liquid is 0.05-2% of the volume of port wine stock solution. The preparing technique comprises the following steps: utilizing spirulina and grapes to be raw material, removing fishy smell of the spirulina, breaking wall, and decolourizing, enriching effective component, and getting spirulina primary liquid, fermenting grapes to brew grape brut, blending and allocating spirulina primary liquid and grape brut according to a certain propostion, and preparing spirulina port wine through storing, clarifying and sterilizing. The wine integrates the health care functions of spirulina and port wine on the basis of keeping the original flavor of port wine, which can enhance body immune in long term drinking with a certain amount, and the wine is health, which prolongs life, and has the health-care and therapeutic functions of adjusting blood sugar, adjusting blood fat, resisting apolexis, resisting tumour and the like.