Method for extracting chlorophyll from spirulina by using supercritical fluid

A technology of supercritical fluid and spirulina, applied in the field of separation, can solve the problems of large chlorophyll loss, high extraction temperature and low extraction yield.

Inactive Publication Date: 2010-02-17
HANGZHOU INST OF ADVANCED MATERIAL BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these traditional methods have a long process, many times of leaching, transferring, and washing with filtered water, resulting in a large loss of chlorophyll and low extraction yield; if they are not fully washed, they will contain many impurities; and most organic solvents are harmful to health and unfriendly to the environment. , solvent residues are unavoidable; the extraction temperature is high and the time is long, and the chlorophyll is easily damaged, especially in the extraction process, it is often accompanied by reactions such as magnesium removal and esterification. As a result, not only the product quality is difficult to control stably, but also the chlorophyll The natural characteristics bring a series of uncertain factors to the future application

Method used

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  • Method for extracting chlorophyll from spirulina by using supercritical fluid

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Effect test

Embodiment 1

[0018] Put the spirulina powder into the extraction kettle F, input carbon dioxide into the extraction kettle F through the steel cylinder A, control the extraction conditions by using the control system B, in which the pressure is increased to 25Mpa by the high-pressure pump C, and the temperature is set by the temperature control system D The temperature is 50°C, the flow rate of carbon dioxide is 60g / h, extract for 5 hours, and use high-pressure pump E to add absolute ethanol as entrainer in the extractor. The amount of entrainer is 0.15L of absolute ethanol per gram of raw material. Chlorophyll is obtained in device G. Chlorophyll was measured by spectrophotometry, and the yield of chlorophyll was 0.89%, wherein the concentration of chlorophyll a accounted for 78% of the total chlorophyll concentration, and the concentration of chlorophyll b accounted for 22% of the total chlorophyll concentration.

Embodiment 2

[0020] Put the spirulina powder into the extraction kettle F, input carbon dioxide into the extraction kettle F through the steel cylinder A, control the extraction conditions by using the control system B, in which the pressure is increased to 30Mpa by the high-pressure pump C, and the temperature is set by the temperature control system D The temperature is 55°C, the flow rate of carbon dioxide is 100g / h, extract for 4 hours, add absolute ethanol as entrainer in the extractor with high-pressure pump E, and the amount of entrainer is 0.2L of absolute ethanol per gram of raw material. Chlorophyll is obtained in device G. Chlorophyll was measured by spectrophotometry, and the yield of chlorophyll was 1.02%, wherein the concentration of chlorophyll a accounted for 80% of the total chlorophyll concentration, and the concentration of chlorophyll b accounted for 20% of the total chlorophyll concentration.

Embodiment 3

[0022] Put the spirulina powder into the extraction kettle F, input carbon dioxide into the extraction kettle F through the steel cylinder A, control the extraction conditions by using the control system B, in which the pressure is increased to 40Mpa by the high-pressure pump C, and the temperature is set by the temperature control system D The temperature is 50°C, the flow rate of carbon dioxide is 120g / h, extract for 5 hours, and use high-pressure pump E to add absolute ethanol as entrainer in the extractor. The amount of entrainer is 0.25L of absolute ethanol per gram of raw material. Chlorophyll is obtained in device G. Chlorophyll was measured by spectrophotometry, and the yield of chlorophyll was 1.19%, in which the concentration of chlorophyll a accounted for 81% of the total chlorophyll concentration, and the concentration of chlorophyll b accounted for 19% of the total chlorophyll concentration.

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Abstract

The invention relates to a method for extracting chlorophyll from spirulina by using supercritical fluid, which comprises the following steps: placing 0.5-3g of spirulina powder into an extraction kettle, introducing carbon dioxide, boosting the pressure to 20-40MPa through a high pressure pump, setting the temperature to be 30-60 DEG C and the flow of the carbon dioxide to be 50-120g / h, extracting for 1-5h, obtaining the chlorophyll in a separator connected with the extraction kettle, or adding absolute ethyl alcohol into an extractor to be used as an entrainer, wherein the use amount of theentrainer is as follows: 0.05-0.15L of absolute ethyl alcohol is adopted in 1g of the spirulina powder. The method greatly avoids reactions of de-magging, esterification and the like occurring in theprocess of common extraction, farthest keeps the original characteristics of the chlorophyll, has simple procedure, less steps, short production period, high efficiency, complete extraction and no anynew three-wastes, and is beneficial to protecting the environment.

Description

technical field [0001] The invention belongs to the field of separation, and in particular relates to a method for extracting chlorophyll from spirulina by using a supercritical fluid. Background technique [0002] Pigments are commonly used dyes in food, medicine and cosmetics. With the continuous improvement of living standards, people gradually doubt chemically synthesized pigments, and pay more and more attention to the development of natural pigments. Chlorophyll is a natural food coloring allowed in our country and most countries. Chlorophyll is widely distributed in plants, and its derivatives have various biological activities in medicine, such as promoting wound healing, anti-ulcer, anti-tumor, anti-microbial, anti-cancer, liver protection, and anti-anemia. Chlorophyll is the green pigment in the photosynthetic membrane, which is the main component for capturing light in photosynthesis. There are four types of chlorophyll a, b, c and d. All plants that release o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22C09B61/00
CPCY02P20/54
Inventor 吴浩
Owner HANGZHOU INST OF ADVANCED MATERIAL BEIJING UNIV OF CHEM TECH
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