322 results about "Chlorophyll a" patented technology

The invention discloses a method for forecasting blue-green algae water bloom in a large-scale shallow lake within 72 hours. The method comprises the following steps of: building a content forecasting model of chlorophyll a in a water body and a probability forecasting model of water bloom generation according to an in-situ growth rate and drift rate parameters of blue-green algae; analyzing instant content of the chlorophyll a in a monitoring water area; distributing and acquiring meteorological information data in a monitoring period; inputting the built models; and outputting a predicted value of the concentration of the chlorophyll a in the monitoring water area in the next 72 hours and the water area and the probability of water bloom generation. The method disclosed by the invention has the advantages of strong operability, easily acquired parameters used for forecasting and high timeliness, and can be used for rapidly and accurately predicting breakout of the blue-green algae water bloom and forecasting the blue-green algae water bloom in a waterhead site and a vital landscape water area.

The invention relates to a density forecast method of the chlorophyll a stemmed from a water body of the BP neural network. The density forecast method comprises the following steps: (1) the chlorophyll a in the tested water body and the value of other correlative water quality index which influences the chlorophyll a are acquired as the examination data. (2) The neural network of an error back propagation is established. (3) The neural network is trained and tested. (4) The neural network which passes the test is utilized to forecast the chlorophyll a in the water body. Other water qualities which influence the chlorophyll a are: Ammonia nitrogen, total nitrogen, total phosphorus, orthophosphate, permanganate index, temperature, dissolved oxygen, pH, suspension, five-day biochemical oxygen demand. The step (1) also comprises a normalization process. The data of the chlorophyll a and other ten water quality indexes are between -1 and +1 after the data of the chlorophyll a and other ten water quality indexes are normalized. The neural network comprises an input layer, an intermediate layer and an output layer. The invention can establish a forecast model related to the chlorophyll a, just needing the experiment which has the limited times. The chlorophyll in the river can be accurately and quickly forecasted through the computer simulation experiment and the science forecast.

Provided herein are exemplary vectors for transforming algal cells. In exemplary embodiments, the vector comprises a Violaxanthin-chlorophyll a binding protein (Vcp) promoter driving expression of an antibiotic resistance gene in an algal cell. Embodiments of the invention may be used to introduce a gene (or genes) into the alga Nannochloropsis, such that the gene(s) are expressed and functional. This unprecedented ability to transform Nannochloropsis with high efficiency makes possible new developments in phycology, aquaculture and biofuels applications.

The invention discloses a chlorophyll a concentration inversion method and system. The method comprises the following steps of: S1: acquiring multispectral remote sensing data of an environment satellite No.1; S2: judging whether the date of the acquisition of the multispectral data exists or not; if the date exists and is matched with a chlorophyll a concentration seasonal inversion model, selecting a chlorophyll a concentration seasonal inversion model according to the date to calculate chlorophyll a concentration; otherwise, selecting a waveband-optimization-based chlorophyll a model to calculate the chlorophyll a concentration. Through the utilization of the multispectral and hyperspectral data of the environment satellite No.1, the problem that an inversion model is limited by time and space can be solved, and the automatic operation and service operation of a computer can be realized.

The invention provides an MODIS satellite high-precision monitoring method for chlorophyll-a in a eutrophic lake water body. The method comprises the following steps: screening a chlorophyll-a evaluation index (BNDBI) which is sensitive to the concentration change of chlorophyll-a and not affected by highly suspended matters, thin cloud and solar flares; acquiring the quantitative relationship between BNDBI and the concentration of chlorophyll-a on the basis of biological optical model simulation; acquiring a chlorophyll-a inversion algorithm based on ground measured spectrum (Rrs) and BNDBI by referring to water body spectral information and corresponding water body chlorophyll-a concentration of 2013-2014 lake field monitoring; acquiring the quantitative relationship between the ground monitoring and remote sensing reflectance ratio (Rrs) and Rrc after simulated Rayleigh scattering correction by simulating different aerosol types and thicknesses and different solar elevation angles, satellite observation angles and azimuth angles; and extending the chlorophyll-a inversion algorithm based on ground measured spectrum data to satellite image data after Rayleigh scattering correction. Based on the method, inter-annual and inter-monthly change rules and spatial distribution of chlorophyll-a in a eutrophic lake can be acquired accurately.

The invention discloses an analysis method of remote sensing inversion of water color parameters of inland class II water. According to the method, inherent optical quantity of the class II water is inverted with an improved QAA (quasi-analytical algorithm), and concentration inversion of the water color parameters such as chlorophyll a and suspended matters is realized based on the inverted inherent optical quantity of the class II water. The method comprises specific steps as follows: (1), data of remote sensing reflectance above the surface of the water is input, parameter inversion of the inherent optical quantity of the water is realized based on the improved QAA, and absorption coefficients and scattering coefficients of the water and absorption coefficients of phytoplankton are acquired; (2), concentration data of the chlorophyll a and concentration data of the suspended matters of the water at a sampling point are input, a chlorophyll a concentration quantitative inversion model is established according to the concentration data of chlorophyll a and the absorption coefficients of the phytoplankton, and a suspended matter concentration quantitative inversion model is established according to the concentration data of the suspended matters and the absorption coefficients of the water after removal of pure water; (3), hyperspectral data finishing atmospheric correction are input, and concentration inversion of the water color parameters of the inland class II water in a monitoring area is realized according to the step (1) and the step (2).

The invention discloses a method for evaluating flood tolerance of the maize variety. The method comprises the following steps of 1, after a pot is drowned, performing evaluation on four indexes, such as the color of a leaf, the form of the leaf, the color of a stem and the form of a root to preliminarily determine the flooding tolerance level; 2, performing two-sample pairing statistic analysis method on 15 shapes subjected to preliminary screening in the first step, and taking the strain height, the net photosynthetic rate, the green area, the root biomass, the root growth rate, the content of chlorophyll a, the total content of chlorophyll and an SPAD (soil and plant analyzer development) value as flood tolerance degree evaluation indexes according to the waterlogging influence degree; and 3, performing clustering analysis on the authentication indexes obtained by the second step, and classifying the varieties with different flood tolerance degrees into a flood tolerance hybrid and a non flood tolerance hybrid near to 27 according to genetic distances of all property clusters. The method for quickly and easily obtaining the flood tolerance evaluation indexes in a maize seedling stage is established according to the form change of leaves and stems above the ground as well as the physiologic and morphologic expression of plants, so that a basis is supplied to correct selection of the maize variety in an easily flooding region.

The embodiment of the invention provides a chlorophyll remote sensing inversion device and a chlorophyll remote sensing inversion method. Based on sensitivity analysis of a spectral characteristic of chlorophyll a, an improved chlorophyll a inversion model is formed, and influence of complex optical property of a nearshore water body on the chlorophyll a is integrally considered; meanwhile, an HICO high spectral image is pre-processed to obtain an image mean value spectrum of an experimental sample region. The constructed chlorophyll a inversion mode is applied to the pre-processed image for chlorophyll a inversion. According to the embodiment of the invention, the inversion precision of the chlorophyll a is hugely increased.

The invention aims to develop a flow-cytometry-based method for rapidly measuring heterotrophic bacteria in a eutrophic lake. The method is a flow-cytometry-based bacterium counting method. The method comprises the following steps of fixing a sample, performing ultrasonic processing, filtering, performing SYBR Green I dyeing, detecting the heterotrophic bacteria by utilizing a flow cytometer, and acquiring forward and lateral scattering light, an SYBR Green I green fluorescence signal and a chlorophyll a red fluorescence signal of autotrophic plankton, wherein the SYBR Green I green fluorescence signal is used for setting a threshold value; and eliminating the influence of the nannoplankton on bacterium detection by utilizing the forward and lateral scattering light, separating the plankton from abiological particles and cellular debris according to the intensity of the SYBR Green I green fluorescence signal, and separating the bacteria from phytoplankton according to the intensity of the chlorophyll a red fluorescence signal of the autotrophic plankton, thereby obtaining the accurate number of the heterotrophic bacteria. The method has the time-saving and labor-saving effects, the large-scale ecological survey can be developed, and the counting precision and accuracy of the bacteria are improved.

The invention relates to a semi-analytical method for realizing inversion of water body chlorophyll alpha concentration, which comprises the steps of: (1) reading the data of chlorophyll alpha, water body spectroscopic data and high-spectrum remote sensing data; (2) utilizing the optical characteristics of the water body containing the chlorophyll alpha to establish semi-analytical model of the chlorophyll alpha concentration; (3) on the basis of the data in the step (1), adopting a method of enumerating by band and linear iteration, and calculating the best wave band and model parameter of the semi-analytical algorithm; and (4) on the basis of the steps (1) and (3), extracting the space distribution information of the chlorophyll alpha concentration from high-spectrum remote sensing image.

The invention discloses an algae-controlling laterite compound flocculant as well as a preparation method and applications thereof. The method comprises the following steps: mixing 64.52-86.42% of laterite, 7.41-19.35% of polyaluminium chloride and 6.17-16.13% of ferric trichloride, and then milling to obtain the algae-controlling laterite compound flocculant. Compared with the methods by independently adding ferric trichloride or polyaluminium chloride, the algae-controlling laterite compound flocculant can obtain higher turbidity and higher chlorophyll a clearance rate, i.e. favorable effects for clearing algae and purifying water. Furthermore, with similar turbidity and chlorophyll a clearance rate, the quantities of the polyaluminium chloride and the ferric trichloride contained in thealgae-controlling laterite compound flocculant are lower than those of the polyaluminium chloride and the ferric trichloride which are independently used so as to save the cost and enhance the safetyof the flocculant in utilization. The algae-controlling laterite compound flocculant has the advantages of simple preparation procedure, moderate cost and favorable effects for clearing algae and purifying water, and is especially suitable for water bloom emergency treatment.

A method for classifying underwater original position of polytoplankton by detecting concentration of polytoplankton includes setting a groove at one side of closed pressure box and a light shading cover at external side of said box; erecting LED array formed by parallel-arranged LED with center wavelength of 450nm, 525nm, 570nm 590nm and 620nm in light path at side surface of said groove; dividing polytoplankton to be five light spectrum set as green, blue, brown, red and mixture; measuring 685nm florescent intensity of five different exciting wavelength to calculate out chlorophyll concentration of each light spectrum according to known coefficient.

The invention provides a remote sensing monitoring method for the concentration of chlorophyll a in an inland water body based on a sectional coordination model. Aiming at the defects and disadvantages in remote sensing monitoring technology for of the concentration of the chlorophyll a, the invention provides the method which combines the advantage that an optical physical mechanism of the water body of the chlorophyll a can perform quantitative theoretical analysis and the advantages of simple calculation and high accuracy of a statistical correlation model. The method is characterized in that the method comprises four units, namely a threshold determination unit, a sectional coordination model unit, a model connection unit and a model calculation unit. The technical method jointly formed by the four units can effectively improve the inversion accuracy of remote sensing data to the chlorophyll a.

The invention provides a diagnosis and evaluation method for the water quality of an eutrophic shallow lake subjected to ecological restoration, which comprises the following steps of: selecting two factors, i.e. water transparency and chlorophyll a concentration which are important for water-quality improvement and stabilization in the ecological restoration process of the eutrophic shallow lake, comprehensively considering the interaction between the two factors, and establishing a water-quality diagnosis index-lake water quality index (LQI) of the eutrophic shallow lake subjected to the ecological restoration. The LQI can be used for quantitatively describing the conversion between the clean-water steady state and the muddy-water steady state of the eutrophic shallow lake so as to provide a visual and convenient diagnosis method for the water quality of the eutrophic shallow lake subjected to ecological restoration and also provide a scientific and reasonable reference basis for the drafting of management measures of the eutrophic shallow lake subjected to ecological restoration and the selection and the application of a subsequent ecological engineering method.

The invention provides a retrieval method and a retrieval device of chlorophyll a concentration of inland case II water, wherein the retrieval method of the chlorophyll a concentration of the inland case II water comprises the steps of firstly, classifying first remote sensing reflectance spectra to obtain a plurality of second remote sensing reflectance spectra taking different components as main components; calculating the weight matrixes of all the second remote sensing reflectance spectra, selecting all characteristic wave bands and calculating the chlorophyll spectral indexes; finally, carrying out weight fusion on the chlorophyll spectral indexes under all categories of all the spectra by weight to obtain weighted chlorophyll index, and fitting an inversion model to obtain the chlorophyll a concentration. Due to the method of firstly classifying and then carrying out weighted inversion, the limitation on the retrieval method caused by the area and the time can be reduced, and the smoothness and the stability of inversion results can be improved, and therefore, when the chlorophyll a concentration of a certain second remote sensing reflectance spectrum is inversed subsequently, the chlorophyll a concentration can be obtained by inversion through the inversion model corresponding to the second remote sensing reflectance spectrum, so that the universality of the method is improved.

The invention discloses a hydrobiontic algae chlorophyll measuring method which consists of, using spectrophotometry method to measure the algae chlorophyll content in the water, filtering subaqueous algae with cellulose acetate microporous filtering film, carrying out interception to the algae cellulose acetate through immersion with 90% ethanol, extracting the chlorophyll in algae cell into ethanol, centrifuging the tobacco extract to obtain the clarified solution, using 90% ethanol as reference, determining absorbancy of the tobacco extract at 630, 647, 664 and 750 nm with a spectrophotometer, calculating content of chlorophyll a, b, c in the water sample by utilizing the measured data.

The invention provides an eutrophic lake total algae storage (token state: total chlorophyll a, dimension: t) remote sensing evaluation method comprising the steps that the quantitative relation between NDBI and concentration of water surface chlorophyll a is acquired based on bio-optical model simulation and actually measured data and popularized to MODIS satellite image data through Rayleigh scattering correction; the water depth spatial distribution situation of the Chaohu Lake is determined based on the water level of the Chaohu Zhongmiao Temple and the Chaohu brake of the same day and Chaohu underwater DEM; the lake NDBI threshold is judged through screening based on actually measured profile empirical data, and the evaluation method for total storage of algae in a unit water column under the algae bloom and non-algae bloom conditions is constructed; and the evaluation method for total storage of algae in a unit image element based on MODIS satellite images is constructed. The annular and monthly change rule of eutrophic lake total algae storage and spatial distribution thereof can be accurately acquired based on the method.

The invention discloses a submerged macrophytes restoring process guided by water conservation fishery. By stocking silver carp and bighead carp and reducing COD (chemical oxygen demand), TP (total phosphor), chlorophyll and other chemical indexes of the water body, the process effectively reduces the algae biomass in the water body, improves the water body transparency, and achieves the aim of preventing algal bloom and improving the water quality, so an excellent growing condition is provided for aquatic macrophytes (in particular, the submerged macrophytes); and by adding fish and other key organisms eating attaching organisms, which is a key link in a food chain, the attaching organisms growing vigorously on the surface of the submerged macrophytes can be removed in time, and the aim of restoring the submerged macrophytes can be achieved.

The invention relates to an offshore water chlorophyll a concentration remote sensing inversion method based on an improved random forest. remotely sensing image data by utilizing a time sequence; combining timing buoy observations, The method comprises the following steps of: establishing an intelligent remote sensing inversion model to carry out remote sensing inversion on the chlorophyll a concentration of an offshore water area by taking time continuous space sparse compensation as a modeling strategy and adopting an improved random forest-double-weight random forest method to obtain a very good inversion result, and visually and accurately displaying the spatial distribution of the chlorophyll a concentration of the offshore large range. The invention can provide a macroscopic, continuous and effective method for monitoring the concentration of chlorophyll a in the near-shore water body, can make up the deficiency of the traditional chlorophyll a concentration monitoring, and hashigher practical value.

The invention provides a short-term forecasting method for eutrophication shallow lake algae source lake flooding. The method comprises the steps that a plurality of monitoring points are set in a water area where shallow lake flooding is likely to happen so as to measure meteorological parameters and water environment parameters; a space mesh dividing and interpolation algorithm is used for interpolating the water environment parameters of the monitoring points to a whole lake, and space distribution is used as the initial condition of a water quality numerical model; the wind field time and space distribution in the next three days is used as model external stress, a three-dimensional water power-water quality numerical model is driven to conduct calculation, and the time and space distribution of the shallow lake chlorophyll a and the dissolved oxygen concentration in the next three days is obtained; the time and space distribution of the chlorophyll a and the dissolved oxygen concentration is used for being combined with the meteorological parameters, so that a probability empirical formula is established, and the probability of lake flooding in the water area where the lake flooding is likely to happen in the next three days is calculated. For the water area where the lake flooding probability is large, the position and area of lake flooding are further determined.

The invention discloses a method for detecting and analyzing the particle size distribution of phytoplankton cells. 1) Pretreatment is performed on samples containing phytoplankton cells, and the cell concentration is adjusted to a suitable range; 2) According to chlorophyll a autofluorescence, the phytoplankton cells were screened out by cytometer analysis; 3) Under the same test conditions, fluorescent microspheres with different particle sizes were used to fit the Φ-FSC standard curve of forward scatter signal intensity (FSC) and particle size value Φ , to obtain a fitting function that converts the FSC signal intensity into a particle size value; 4) On the basis of step 2), generate a peak diagram of the particle number distribution of the phytoplankton cells injected after screening and the FSC distribution, and on the basis of step 3), Calculate the particle size corresponding to the mean value of the cell count in the injected sample to obtain the particle size distribution of the phytoplankton cells. The invention is easy to operate, can obtain the particle size distribution of phytoplankton in a statistical sense, and can be used to carry out research on particle size composition, ecological structure and the like of phytoplankton populations.