367 results about "Chlorophyll content" patented technology

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention provides application of molybdenum disulfide nanoparticles in preparation of a tomato leaf fertilizer, and particularly relates to the technical field of tomato fertilizers. The molybdenum disulfide nano-particles act on tomato plants in a foliage spraying manner, so that the growth of the tomato plants is promoted and / or the fruit quality is improved. The molybdenum disulfide nanoparticles are used as the foliar fertilizer to significantly increase the plant height and stem diameter of tomatoes, increase the chlorophyll content and trace element content of plants, increase the vitamin C content, soluble sugar content and soluble solid content of tomato fruits, and significantly increase the accumulation of Mo and S of plants through the sulfur-molybdenum synergistic effect, so that the yield of the tomatoes is increased. A scientific basis is provided for reasonable application of the nano molybdenum fertilizer, and a way is opened up for high-yield and high-quality cultivation of tomatoes.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention relates to the technical field of agricultural quantitative remote sensing and intelligent agriculture, and discloses a multi-source remote sensing data radiation correction and chlorophyll content cross-platform inversion method, which comprises the following steps: synchronously obtaining ground actual measurement, unmanned aerial vehicle and satellite remote sensing data, calculating vegetation indexes, and screening sensitive characteristic variables by using Pearson correlation analysis; establishing a radiation correction model by adopting a ratio averaging method, performing radiation normalization processing on satellite data by taking unmanned aerial vehicle data as a reference, and eliminating sensor response differences; an SPAD inversion model is constructed and optimized based on Z-score standardization, the SPAD inversion model is migrated and applied to corrected satellite data, a regional scale chlorophyll distribution diagram is generated through pixel-by-pixel calculation, and then a differentiated farmland management strategy is formulated. According to the method, the difficulty of multi-source data collaboration is solved, the regional scale crop chlorophyll monitoring precision is improved, and scientific decision support is provided for large-area crop fertilization.

The invention provides a bamboo forest growth monitoring method and device based on a multispectral image, and relates to the technical field of remote sensing monitoring, and the method comprises the steps: obtaining a multispectral image of a to-be-detected bamboo forest through a multispectral camera, and enabling the multispectral camera to be carried on an unmanned aerial vehicle; based on the multispectral image, growth indexes of the bamboo forest to be detected are obtained, and the growth indexes comprise the height of bamboo plants, the number of the bamboo plants, chlorophyll content indication values of the bamboo plants and biomass of the bamboo plants; based on the growth indexes, determining the bamboo forest growth vigor. According to the bamboo forest growth vigor monitoring method based on the multispectral image, the growth vigor of the bamboo forest is comprehensively analyzed through all growth indexes, and accurate and efficient growth vigor monitoring of the large-area regional bamboo forest is achieved.

The invention discloses a potato seedling grading environment parameter dynamic optimization regulation and control method, and relates to the technical field of agricultural informationization. Based on an Internet of Things sensor deployed in a target area, stem diameter, chlorophyll content and plant height data of potato seedlings are collected, the seedlings are divided into weak seedlings, middle seedlings and strong seedlings by adopting K-means clustering, and the weak seedlings, the middle seedlings and the strong seedlings are classified into a weak seedling classification model, a middle seedling classification model and a strong seedling classification model; setting the duration of the initial light period; and carrying out gradient photoperiod test on each type of potato seedlings by taking the stem elongation rate and the chlorophyll content as constraint conditions. Through grading photoperiod modeling and ant colony algorithm dynamic optimization, the limitation of traditional fixed photoperiod regulation and control is broken through, the initial photoperiod range is set based on the seedling physiological difference, and the photoperiod-growth rate response curved surface model is constructed in combination with the stem elongation rate and chlorophyll content constraint conditions. The influence of different photoperiod combinations on seedling growth is quantified, accurate matching of photoperiods and seedling requirements is ensured, excessive growth or premature senility is avoided, and the growth rhythm stability and the resource utilization efficiency are improved.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention discloses a SiERF109 gene for regulating drought resistance, salt tolerance and flavonoid accumulation of millet and application of the SiERF109 gene, and belongs to the technical field of plant breeding. According to the invention, a gene SiERF109 with a nucleotide sequence shown as SEQ ID NO.1 is separated and cloned from millet, and fluorescent quantitative expression analysis shows that SiERF109 participates in drought and salt stress response of millet. After the gene is over-expressed in foxtail millet, in drought and salt stress experiments, compared with a wild type strain, the survival rate and chlorophyll content of a strain over-expressed with the SiERF109 gene are remarkably increased, the flavonoid content is increased by 33.5%-39.5%, and active oxygen accumulation is remarkably reduced; it is shown that the SiERF109 gene and the protein coded by the SiERF109 gene can remarkably improve the drought resistance, the salt resistance and the flavonoid content of the plant.

The invention provides a salt-tolerant gene, namely a PrABC1 gene and application thereof, and also provides a vector containing the gene, an expression cassette, a recombinant cell, a transgenic plant and a method for producing the transgenic plant. Specifically, the gene has a nucleotide sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The plant growth regulator can be used for improving the tolerance of plants to a salt stress environment, relieving the adverse effects of a high-salt environment on the germination rate and root length of the plants, reducing the water loss rate of the plants under the high-salt stress condition and reducing the reduction of the chlorophyll content.

The invention discloses a method for evaluating and screening salt tolerance of triticale germplasm resources in a germination stage, and belongs to the technical field of evaluation and screening of plant salt tolerance, salt stress concentration screening: triticale seeds are treated by NaCl solutions with different concentrations, indexes such as survival rate, plant height and overground part fresh weight are measured, the NaCl solution with the concentration of 200 mmol. L <-1 > is determined as the salt tolerance evaluation appropriate concentration, and the salt stress concentration is determined as the salt tolerance evaluation appropriate concentration. Salt tolerance evaluation test: carrying out salt stress treatment by adopting a 200mmol. L <-1 > NaCl solution, determining phenotypic indexes such as survival rate, plant height, fresh weight of overground part, root length and chlorophyll content, and physiological and biochemical indexes such as catalase, peroxidase and superoxide dismutase activity and malondialdehyde content, and carrying out principal component analysis, membership function analysis and clustering analysis to obtain a salt tolerance evaluation result. Calculating a salt tolerance comprehensive evaluation value, and screening salt tolerance triticale germplasm resources.

The invention relates to the field of gene engineering and molecular breeding, and discloses application of a ZmTCP5 gene in regulation and control of heat resistance of corn. According to the invention, three types of knockout mutants of the gene are obtained by using a CRISPR / Cas9 technology, and three overexpression materials are created. At normal temperature (25-28 DEG C), the material has no obvious phenotypic difference with a wild type; however, under high temperature stress (45 DEG C), the ZmTCP5 knockout mutant has enhanced heat resistance, reduced reactive oxygen species (ROS) accumulation, and significantly increased ROS scavenging enzyme activity, chlorophyll content and photochemical efficiency; on the contrary, the ZmTCP5 overexpression strain is more sensitive to heat stress, which indicates that the ZmTCP5 negatively regulates the heat resistance of the corn. The research provides a theoretical basis for analyzing a heat-resistant molecular mechanism of the corn, and also provides an important gene resource for creating a new germplasm of the heat-resistant corn.

The invention provides a fresh tobacco leaf chlorophyll content integrated model prediction method, medium and system, and belongs to the technical field of tobacco leaf detection.The method comprises the steps that transmission spectrums of fresh tobacco leaves with different maturity degrees are collected and subjected to black and white correction; dividing the leaf into six areas, collecting spectrums, and calculating an average value; carrying out multiple pretreatments on the original spectrum and dividing a data set; analyzing and establishing a relationship between the chlorophyll content and the spectrum based on a photosynthetic pigment absorption mechanism, and screening key wavelengths; the FMR-NSGA multi-objective optimization algorithm is fused with the F test and mutual information method to screen characteristic wavelengths; dynamically eliminating redundant features by using a recursive feature elimination method; establishing a parameter mapping function, and automatically determining a model hyper-parameter according to the wavelength importance index; a Stacking framework is constructed, hyper-parameters are dynamically optimized by adopting a grey wolf optimization algorithm, the prediction precision is improved, and the technical problem of rapid and accurate prediction of the chlorophyll content of the fresh tobacco leaves is solved.

The invention discloses application of a phosphorus-solubilizing fungus solid bacterial fertilizer in promoting crop growth, and belongs to the field of environment and the technical field of agriculture. The solid bacterial fertilizer comprises penicillium deserticola and / or penicillium fistulosum, the penicillium deserticola and / or penicillium fistulosum are compounded according to the proportion of 1: 1, agricultural waste mushroom bran serves as a main carrier, and the inoculum size of a bacterial solution is 9 wt%. The bacterial fertilizer can effectively activate insoluble phosphorus in soil, significantly improve absorption and utilization of phosphorus by crops, promote plant growth, increase root length and biomass, increase leaf chlorophyll content, enhance photosynthesis capability, reduce dependence on chemical phosphate fertilizer and realize agricultural waste recycling, and has the advantages of high efficiency, environmental protection and low cost.

The invention provides an application of potato laccase StLAC19 in a shade avoidance reaction. The overexpression of the StLAC19 gene can significantly enhance the shading tolerance of potato plants, and effectively improve the lignin content, the osmotic regulation substance content, the chlorophyll content, the stalk strength, the CAT and POD enzyme activity and the PRO and MDA content; on the contrary, interference on expression of the StLAC19 gene results in a contrary result. The result shows that the StLAC19 gene positively regulates and controls the shading reaction of potato plants. The screening of varieties with high expression of the StLAC19 gene is helpful for obtaining materials with stronger shade-avoiding tolerance, so that the gene can be used as a target gene for breeding shade-avoiding tolerance type potato varieties.

The invention discloses a method for determining a toughness and catastrophe threshold value of a functional group of a water ecosystem, and relates to the technical field of water environment ecological engineering.The method comprises the steps that the state of a target water ecosystem is determined according to the total nitrogen content, the total phosphorus content and the chlorophyll content of a water body in the target water ecosystem, the state of the target water ecosystem is eutrophication or non-eutrophication; determining the biomass of phytoplankton based on the target water ecosystem state; utilizing an Ecopath model to construct a static food network relation model of the target water ecosystem; based on the target water ecosystem state, the biomass of the phytoplankton and the static food network relation model, determining a toughness threshold value and a catastrophe threshold value of the biomass of each functional group; each functional group is a plurality of groups for dividing species in the target water ecosystem. According to the method, the water ecosystem functional group toughness threshold value and the catastrophe threshold value which are simple to operate and high in accuracy can be provided.

The invention discloses a rice chlorophyll content prediction method, device, medium and equipment, and relates to the technical field of crop growth, and the method comprises the following steps: obtaining canopy spectral data and physicochemical parameters of rice in a to-be-detected area; dividing the rice canopy into three concentric circle areas based on the leaf inclination angles of different leaves of the rice, and obtaining the layered rice canopy; constructing a hierarchical radiation transfer model HRTM for simulating a rice hierarchical spectrum, and inputting the physical and chemical parameters into the hierarchical radiation transfer model HRTM to generate hierarchical spectrum data; constructing a lookup table LUT based on the physicochemical parameters and the layered spectral data, taking the lookup table LUT as an analog data set, improving the analog data set by adopting a quartile method, and determining analog spectral data; based on the simulated spectrum data, constructing an inversion model for acquiring the chlorophyll content of the rice layers layer by layer; and inputting hyperspectral data to be measured into the inversion model, and determining a corresponding chlorophyll content prediction result.

The invention provides a plant chlorophyll index determination method based on multispectral laser, which comprises the following steps: detecting ambient light intensity of an environment where plant leaves are located to obtain an actual ambient light detection value; according to the actual detection value of the ambient light, a pulse generator is regulated and controlled, and a PWM frequency conversion signal is generated; driving a multi-band laser source based on the PWM frequency conversion signal to enable the laser source to emit laser and form uniform light spots on the surface of the plant leaf; receiving and preprocessing the laser signal reflected by the plant leaf to obtain a pure electric signal; the pure electric signal is converted into a digital quantity, calculation processing is carried out based on the digital quantity and a preset digital quantity, and the reflectivity corresponding to each wave band is obtained; and calculating based on the reflectivity corresponding to each wave band to obtain the chlorophyll index. By adopting the method, related data of the chlorophyll content of the plant can be accurately obtained, and technical support is provided for agricultural scientific management, diagnosis decision and high yield.

The invention provides a charcoal-based microalgae fertilizer as well as a preparation method and application thereof, the method adopts a mode of co-fermentation of charcoal and microalgae to fix microalgae on charcoal, and co-fermentation is performed to prepare the charcoal-based microalgae fertilizer. The biochar-based microalgae fertilizer can significantly improve the crop yield, increase the chlorophyll content of crops, promote healthy growth of plants, optimize the pH value of soil, enhance the water and fertilizer retention capacity of soil, and significantly improve the soil nutrients and soil enzyme activity, and has significant advantages in the aspects of nutrition supply, soil health, environmental protection, crop quality improvement and the like. The biochar-based microalgae fertilizer has two forms of liquid and dry powder, can be flexibly used according to a specific agricultural production mode, has the advantages of easily available raw materials and simple preparation, is a sustainable fertilizer source, can comprehensively improve the soil fertility and the crop stress resistance, reduces the dependence on chemical fertilizers, and reduces the environmental pollution problem in agricultural production.

The invention discloses staphylococcus Z8 for antagonizing pathogenic bacteria of mulberry bacterial wilt and application of the staphylococcus Z8. The Z8 is identified as staphylococcus sp. And is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No.66232, and the preservation time is April 28, 2025. The invention further discloses a preparation method of the staphylococcus Z8 for antagonizing the pathogenic bacteria of mulberry bacterial wilt and application of the staphylococcus Z8 for antagonizing the pathogenic bacteria of mulberry bacterial wilt. The strain Z8 disclosed by the invention can destroy the formation of a biological membrane of the mulberry ralstonia solanacearum, so that the pathogenicity of the ralstonia solanacearum is reduced, and the strain Z8 can be used for preventing and treating the mulberry bacterial wilt. In addition, the strain Z8 does not affect the growth and development of the mulberry, the safety of the strain as a biocontrol strain is embodied, and the root activity and the total chlorophyll content can be improved. An efficient and environment-friendly microbial resource is provided for biological prevention and control of mulberry bacterial wilt, traditional chemical pesticides can be replaced, and environmental pollution is reduced.

The invention provides a spiropolyspora pink and selenium compound nutrient solution and application thereof in biological prevention and control and growth promotion of tomatoes, the prevention and control effects of different treatment combinations on tomato gray mold are studied, disease indexes and prevention and control effects of different treatment combinations are compared, activity changes of stress-resistant related enzymes SOD, POD and MDA are compared, and the disease resistance of the tomato gray mold is improved. A photosynthetic related index is chlorophyll content. The incidence rate of the spiropolyspora roseum and the selenium-enriched nutrient solution in the treatment group is lowest, the prevention and treatment effect is optimal, and the chlorophyll content is highest, so that the compounding agent can obviously improve the photosynthesis of the plants. Different compounding agents have an induction effect on the disease resistance of tomatoes. The selenium content of the compounded tomatoes is obviously higher than that of a control group and a single selenium-rich nutrient solution, the ratio of the optimal selenium-rich combination to the selenium-rich nutrient solution is 2: 3, the selenium content of tomato fruits can reach 52.67 g / kg, and it is indicated that the selenium absorption of the tomatoes can be promoted through the spiropolyspora pink.

The invention relates to the technical field of plant genetic engineering, in particular to an application of a poplar PtbHLH47 gene and an encoding protein thereof in improving the salt tolerance of plants. The invention discloses application of a PtbHLH47 gene to improvement of plant salt tolerance and improvement of chlorophyll content of plant leaves. The nucleotide sequence of the PtbHLH47 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the expression protein of the PtbHLH47 gene is as shown in SEQ ID NO. 2. PtbHLH47 genes are cloned from populus tomentosa leaves, overexpressed PtbHLH47 transgenic populus tomentosa is obtained through an agrobacterium tumefaciens-mediated populus genetic transformation system, salt tolerance evaluation shows that the salt tolerance of the overexpressed PtbHLH47 transgenic populus tomentosa is remarkably improved, particularly, the chlorophyll content in the populus tomentosa leaves under salt stress can be remarkably improved, and the chlorophyll content in the populus tomentosa leaves under salt stress can be remarkably reduced. And gene resources are provided for cultivating salt-tolerant plants.

The present invention discloses the application of the NtIPMD gene in regulating the chlorophyll content of tobacco, and belongs to the field of plant genetic engineering technology. Based on the existing progress in tobacco genetic engineering and the observation and identification of related plant phenotypes, the present invention conducts in-depth research on the NtIPMD gene and finds that it is related to the development of tobacco chloroplasts. The nucleotide sequence of the NtIPMD gene is shown in SEQ ID NO.1. After silencing the gene, the chlorophyll content of the leaves decreases, the growth of the plants slows down, and transmission electron microscopy observation reveals abnormal chloroplast development. The present invention finds that the gene encoding tobacco isopropylmalate dehydrogenase, NtIPMD, plays a key role in regulating the chlorophyll content of tobacco leaves, laying the foundation for clarifying the molecular regulatory mechanism of chlorophyll biosynthesis in tobacco leaves and providing a new precise regulatory gene for regulating the chlorophyll content of tobacco leaves.

The invention provides a peptide-enriched organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizer preparation. The preparation method of the peptide-enriched organic fertilizer provided by the invention comprises the following steps: mixing corn soaking water with aspergillus oryzae seed koji, and fermenting for the first time to obtain the peptide-enriched organic fertilizer; the aspergillus oryzae mold starter is obtained by inoculating aspergillus oryzae YY-21 into a culture medium and fermenting 2. According to the preparation method provided by the invention, the peptide-enriched organic fertilizer with high amino acid nitrogen content, high small peptide content, high soluble protein content and high thallus biomass can be prepared, and when the peptide-enriched organic fertilizer is used for planting lettuce, the plant height, the fresh weight of a single plant, the root length, the leaf number and the leaf area of the lettuce can be remarkably increased; the chlorophyll content, the vitamin C content and the total sugar content in the lettuce can be remarkably improved, and the nitrate content of lettuce leaves can be reduced.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention discloses a gene for degrading chlorophyll as well as an encoding protein and application thereof, and belongs to the fields of molecular biology, plant genetic engineering and horticulture. Protein coded by the gene is located in chloroplast, and chlorophyll degradation can be efficiently promoted under various stress conditions. By performing different treatments on Huangkui tea trees and performing gene function verification in model plants such as nicotiana benthamiana and arabidopsis thaliana, it is found that the CsSGRs gene is subjected to induced expression by factors such as natural aging, abscisic acid, illumination intensity and low temperature, and the gene participates in a chlorophyll degradation approach, so that the functions are redundant. In addition, a bZIP family transcription factor CsABF4 is screened out, and CsSGRs gene expression can be regulated and controlled. The invention provides gene resources and theoretical basis for regulating and controlling the chlorophyll content of plants and improving related characters of plant leaf color, and has potential application value in the fields of plant breeding and gardening.

The invention relates to the technical field of rice planting methods, in particular to a nutrient solution for culturing Guizhou indica rice variety 'Guizhou Red No.1' as well as application and a culture method of the nutrient solution. According to the invention, a nutrient solution specially aiming at 'Guihong No.1' is researched and determined, the nutrient solution is added with micromolecular substances and Br with the final concentration of 0.1-0.6 [mu] M, GABA with the final concentration of 0.3-0.9 mM, melatonin with the final concentration of 0.5-5 [mu] M and salicylic acid with the final concentration of 10-20 [mu] M, and the nutrient solution specially aiming at 'Guihong No.1' can effectively improve the plant height, the biomass (fresh weight), the nitrogen content, the chlorophyll content and the dry weight of a single plant of 'Guihong No.1'. The nutrient solution provided by the invention can solve the technical problem of lack of an effective culture method in the prior art, and can better promote large-scale, intensive and factory planting management and large-area popularization and application of indica rice varieties in Guizhou by utilizing synergistic interaction and advantage complementation among different treatment modes.

The invention belongs to the technical field of biology, and particularly relates to Dendrobium officinale DoSTRK1 protein as well as a coding gene and application thereof. The invention discloses a Dendrobium officinale DoSTRK1 protein, which comprises a protein consisting of an amino acid sequence as shown in SEQ ID NO.6, and a protein which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, is related to plant drought tolerance and is derived from the protein. The invention finds that overexpression of the gene encoding the DoSTRK1 protein can enable an overexpression strain to show stronger tolerance under salt stress of a plant, and specifically, the overexpression strain shows longer roots in a seedling stage and higher chlorophyll content in a growth stage. Under drought stress, the overexpression strain also shows stronger tolerance, and compared with a wild type, the overexpression strain has the advantages that the root length in the seedling stage is increased, and the survival rate in the growth stage is higher.

This invention belongs to the field of post-harvest quality testing technology for agricultural products, specifically relating to a method for predicting the degree of chilling injury in bitter gourd. The method uses *Bitter Gourd 'Du Ruan'* as the research object, employing segmented pre-cooling treatment to measure indicators such as chilling injury index, respiration rate, weight loss rate, firmness, chlorophyll content, malondialdehyde content, and antioxidant enzyme activity during storage. This determines suitable pre-cooling conditions, explores the impact of different pre-cooling settings on its physiological quality and chilling injury development during storage, and constructs a bitter gourd chilling injury prediction model based on key indicators. The method for predicting the degree of chilling injury in bitter gourd provided by this invention offers a theoretical basis and technical support for optimizing post-harvest cold chain processes for bitter gourd, mitigating low-temperature damage, and extending shelf life.

The present disclosure provides isolated Pseudomonas koreensis strains useful for enhancing crop yield, as well as inoculant compositions comprising one or more of the isolated strains, seeds that have been treated with one or more of the isolated strains, and methods of using the isolated strains to enhance root growth, nutrient uptake, chlorophyll content, etc.

The invention relates to the technical field of intelligent agriculture, and discloses a facility vegetable field prediction system and method based on multi-modal vision, and the system comprises a terminal control and data collection system which is used for carrying out the image collection of the growth condition of leafy vegetables in a vegetable field; the data processing system is used for denoising, correcting and registering the images and aligning the images of different modalities in space; and the data analysis system reconstructs a 3D model of the plant by using point cloud data of a depth camera or LiDAR, and constructs an integrated sensing system of a two-dimensional form, a three-dimensional structure, a physiological state and a moisture condition by fusing RGB, hyperspectrum, depth and thermal imaging multi-mode image acquisition equipment. According to the system, the technical bottleneck of one-sided information of a single sensor is effectively overcome, the number of leaf vegetables, the canopy volume, the chlorophyll content, the canopy temperature and key parameters directly related to yield formation can be synchronously obtained, and a solid data foundation is laid for high-precision yield prediction.