268 results about "Antioxidative enzyme" patented technology

Single amino acid compounds and methods for upregulating expression of a gene encoding an antioxidative enzyme, such as superoxide dismutase or catalase, to counteract harmful oxidative effects of reactive oxygen species and other free radicals are described. The single amino acid compounds may be used in compositions and methods to treat or prevent diseases and conditions characterized by undesirable elevation of reactive oxygen species and other free radicals.

The invention provides a stable detection kit of total bile acid. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components: a buffering solution, beta-thionicotinamide adenine dinucleotide, a surface active agent, a stabilizing agent and a preservative; the reagent R2 comprises the following components: a buffering solution, reduction type coenzyme I (NADH), 3alpha-hydroxysteroid, a surface active agent, antioxidant enzyme, a stabilizing agent and a preservative. The stable detection kit provided by the invention has the advantages that since the antioxidant enzyme is added into the reagents, NADH can be stored for a long time, the influence caused by more interference components in the reaction process is eliminated, so that the NADH can be utilized efficiently, the speed of circulating reaction is increased, the detection accuracy is improved, the sensitivity is high, the linear range is wide, simultaneously, the used amount of main reaction materials can be reduced and the cost of the reagents is reduced.

An extract of Rosa rugosa Thunb is disclosed, and contains rose extract (30%) which contains polyose (84.74%), protein (8.57%) and gallic acid derivative (6.69%). It plays such roles as suppressing erythrocyte haematolysis by 100% and peroxidization of tissue lipid by more than 90% and increasing the activity of antioxidant enzyme. It can be used to prepare antioxidant for preventing and treatingdiseases and increasing immunity.

The invention discloses an intelligent nanoparticle drug release system capable of delivering biomacromolecules and small organic molecules to cells, a preparation method of the intelligent nanoparticle drug release system, and application of the intelligent nanoparticle drug delivery system in cell delivering of biomacromolecules and small organic molecules (including drugs). By delivering biochemical substances of drug molecules, cell nutrients and cell expellant, physiological metabolism and pathological metabolism are intervened. Molecule delivery of an embodiment of the intelligent nanoparticle drug delivery system includes but is not limited to the following molecules: telomerase cell delivery; antioxidant enzyme (such as SOD (superoxide dismutase) and HPX (hydrogen peroxidase)) cell delivery; cancer suppressor protein (such as p53 and PTEN(phosphatase and tensin homolog deleted on chromosome ten)) cell delivery; and organic chemical and biochemical small molecules (such as atorvastatin-lipitor and coenzyme Q-10) cell delivery. The molecule slow release and control of the nanoparticle drug delivery system has uniqueness and targeting so that the nanoparticle drug delivery system is intelligent.

The invention relates to a method for improving antioxidant enzyme activity of ryegrass by adopting fine domestic garbage compost under drought conditions. The method comprises the following steps of: uniformly scattering garbage trace fertilizers with grain diameters of 1,200 nanometers, 600 nanometers and 300 nanometers respectively on the surface of soil, wherein the weight part ratio of the grain diameter to the soil is 1: 60-80; then, sowing 0.5 gram of tall fescue, controlling the temperature to be between 20 and 26 DEG C and relative humidity to be between 40 and 60 percent, and illuminating the tall fescue with indoor natural light; and fully watering the tall fescue at the initial sowing stage, weighing the water in an amount which is 55 to 65 percent of the field holding amount every day or every two days after two weeks to maintain the water in a stress range, and measuring each index on the 44th day. The method provides technical support for applying the fine-grain urban domestic garbage compost to a lawn planting system.

The invention discloses preparation of a soybean anti-fatigue biological active peptide, belonging to an extraction processing technology of plant protein. The method comprises the following steps: adding water in high-denaturation soybean meal serving as a raw material to prepare a mixed solution; performing ultrasonic preprocessing on the mixed solution; adding neutral protease into the processed mixed solution to perform enzymolysis so as to obtain enzymatic hydrolysate; performing ultrafiltration treatment on the enzymatic hydrolysate by using a membrane separation technology after the enzymolysis to obtain biological active peptide with high peptide content and molecular weight of less than 5 kDa; then performing separation and purification by using anion exchange chromatography and reversed-phase high-performance liquid chromatography; and finally performing freeze drying to obtain the soybean anti-fatigue biological active peptide. The soybean anti-fatigue biological active peptide prepared by the preparation method disclosed by the invention has higher autioxidant enzyme activities and lower malondialdehyde (MDA) content after movement of a mouse; and results show that the soybean peptide has the functions of reducing cell injury caused by lipid oxidation to have an anti-fatigue effect by removing free radicals produced in the body and inhibiting the generation of lipid peroxides.

The invention relates to the field of biology and in particular discloses a method for preparing organic selenium-enriched lactic acid bacteria. The method is characterized by comprising the following steps: a, inoculating lactic acid bacteria to a culture medium in a conventional mode, and performing culture amplification for 24-36 hours; b, adding a sodium selenite solution with the mass concentration of 5-8 percent into fermentation liquor in the step a, so that the selenium content of the fermentation liquor is 30-90mg/L, and continuously culturing at a constant temperature for 24-36 hours; and c, centrifuging the bacteria liquid obtained in the step b, washing for 5 times by using stroke-physiological saline solution, centrifuging, removing the supernatant, and obtaining the organic selenium-enriched lactic acid bacteria preparation. According to the production method, the oxidation resistance and the immunologic function of the normal organism can be improved, the lipid peroxidation reaction of hepatic fibrosis cells is intervened, and particularly, the advantages of an antioxidant enzyme activity system are obviously improved.

The invention is applicable to the technical field of nutritional health-care food, and provides a skin beautifying composition based on a collagen structure, enzyme inhibition and oxidative stress as well as a preparation method. The skin beautifying composition comprises an anti-sugar and anti-aging component, an anti-enzyme activity component and an anti-oxidative stress component, wherein the anti-sugar and anti-aging component comprises 0.01-0.15 part of sodium hyaluronate and 1-5 parts of collagen peptide, the anti-enzyme activity component comprises 0.01-0.5 part of a pomegranate extract and 0.005-0.25 part of N-acetylneuraminic acid, and the anti-oxidative stress component is prepared from 0.06-0.3 part of a grape seed extract and 0.02-0.1 part of strawberry seed powder. The composition disclosed by the invention can improve skin problems such as external aging saccharification, wrinkles, dark skin color and melanin accumulation, continuously regulate biological metabolism of an internal body, enhance the activity of antioxidant enzyme, inhibit oxidative stress reaction, eliminate the level of active oxygen, prevent UV (ultraviolet) damage, increase cell viability and repair power, and protect the dermis layer to achieve the effects of skin whitening, oxidation resistance and aging resistance.

The invention relates to the technical field of collagen extraction, in particular to a preparation method of codfish skin collagen peptide. The method comprises the following steps: mixing codfish skin with water, and performing extraction at 90-95 DEG C for 2-2.5 hours to obtain a mixed material; adding trypsin for enzymolysis, wherein the mass percent of the added trypsin is 4%-5.5%, and the enzymolysis time is 2-3 h; performing inactivating and centrifuging to obtain a supernatant; performing decolorizing and deodorizing with activated carbon, and performing filtration with a plate frame to obtain a decolorized solution; and performing filtration by an ultrafiltration membrane, and then carrying out ion exchange, concentration and freeze drying to obtain the cod skin collagen peptide.According to the method disclosed by the invention, the extraction rate of the collagen peptide can reach 94%-95%. The molecular weight is mainly below 1000Da, and the content is as high as more than80%. The collagen peptide is mainly composed of a polypeptide mixture containing 2-10 amino acids, and can remarkably increase the content of hydroxyproline and collagen in the skin of a model mouse,enhance the antioxidant enzyme activity in human blood and achieve the effect of delaying senescence.

The invention discloses a research of influence of heavy-metal cadmium stress on murraya paniculata, and relates to the technical field of heavy metal technical research. The research comprises the following steps of experiment material preparation, experiment methods determination, data processing, result analysis and conclusion research. Murraya paniculata under cadmium stress is free of death or poison and exhibits strong vitality, and antioxidase in vivo is induced to eliminate the damage of generated active oxygen to cell membranes, so that plant cells are protected from stress. Various chlorophyll fluorescence parameters also reflect that the murraya paniculata are not subjected to large cadmium stress, and photosynthesis can be normally carried out. When the content of cadmium in the murraya paniculata is measured, cadmium with certain accumulation amounts is contained in roots, stems and leaves, but the accumulation amounts do not reach a standard of cadmium-hyperenrichment plants, and the murraya paniculata do not belong to cadmium-hyperenrichment plants. Consequently, murraya paniculata can normally grow under cadmium stress, active oxygen can be normally removed by the antioxidase in the murraya paniculata, photosynthesis is not influenced, a certain extraction and enrichment capability on cadmium in soil is achieved, and the murraya paniculata has relatively strongtolerance.

Peptide compounds and methods for upregulating expression of a gene encoding an antioxidative enzyme, such as superoxide dismutase or catalase, to counteract harmful oxidative effects of reactive oxygen species and other free radicals are described. The peptide compounds may be used to treat or prevent diseases and conditions characterized by undesirable elevation of reactive oxygen species and other free radicals, to upregulate AP-1 gene expression, and to treat pain. The peptide compounds may be used as components of pharmaceuticals and dietary supplements.

The invention provides a cotton seed germination promoter, which is prepared from the following ingredients in percentage by mass: 0.1 to 1.0 percent of chitosan, 0.1 to 0.3 percent of calcium chloride, 0.002 to 0.008 percent of gibberellins and the balance water. After the promoter is diluted, seed soaking is performed. The chitosan is used for improving the activity of various antioxidases in a plant body; the production of various plant pathogenic microorganisms is inhibited; the plant disease-resistant capability is enhanced; the damage of low-temperature threatening and draught threatening to the plant is relieved; the seed dormancy is broken through addition of gibberellins; the effect of promoting the seed germination and young seedling growth can be achieved; the low-temperature injury of seeds in the germination period is relieved; the germination rate of the seeds in adverse environment can be obviously improved; meanwhile, the nutrition elements required by the plant growth and development are increased by using the calcium chloride; each aspect of the plant growth and development is regulated; the content of calmodulin can be improved; the seed activity is enhanced; the emergence rate and the stocking rate of the cotton seeds in low-temperature cold damage environment are finally improved.

The invention relates to a blood substitute and discloses poly hemoglobin cross-linked with antioxidase, and the poly hemoglobin is formed by chemically cross-linking superoxide dismutase and catalase. The poly hemoglobin cross-linked with the antioxidase has lower autoxidation rate and stronger oxidation resistance, can resist exogenous oxidants, does not need cross matching and laboratory detection during the application and has extensive applicability.

The invention discloses a wheat low temperature-resistant conditioning agent and an application method thereof. The wheat low temperature-resistant conditioning agent comprises the following components in parts by weight: 18-27 parts of melatonin, 13-19 parts of potassium humate, 12-17 parts of a-naphthylacetic acid, 8-14 parts of jasmonic acid, 6-14 parts of phytohormone brassinolide, 3-7 parts of cytokinin, 2-8 parts of an antifreeze, 0.3-0.7 parts of antibacterial peptide, 1-5 parts of a dispersant, and 20-30 parts of water. The wheat low temperature-resistant conditioning agent has the beneficial effects that the conditioning agent takes water as a medium, has obvious environment benefit and social benefit, can increase the security; protects a cell membrane, stabilizes the structure of biomacromolecules such as antioxidant enzyme, enhances capability for effectively removing ROS by cells, reduces intracellular water potential and ice point, and enhances the anti-pathogen capability of the wheat; the active ingredient source is abundant, the active ingredient is regenerable, is easily degraded in natural environment, has the characteristics of low cost and convenient usage, and conditioning agent has the advantages of easy popularization and application.

Present invention provides an aqueous composition made from selected biochemical substances that will help with the low temperature tolerance of plants and thus to avoid “chilling injury” and to promote better recovery from such injury, when the solution is applied to crop plants prior to the arrival of cold snap or period of low temperature.

The invention belongs to the traditional Chinese medicine pharmacy field, and relates to an application of leonurine SCM198 in preparation of medicines used for preventing and treating parkinson disease. According to the invention, neurotoxin 6-OHDA induces damage of nerve cells in rats, the result shows that leonurine SCM198 is capable of improving behavior changes of PD model rats, increasing the survival rate of damaged nerve cells, raising antioxidant enzyme superoxide dismutase activity, minimizing excessive generation of active oxygen species, stabilizing mitochondrial membrane potential, minimizing apoptotic cells and regulating the change level of Bax and Bcl2 gene and protein. The result proves that the leonurine SCM198 has protection effect on a PD model, and can be used for preparing medicines for preventing and treating PD.

The invention discloses a method for preserving fresh dates at normal temperature. The method mainly comprises the following steps of: carefully selecting the fresh dates, soaking the fresh dates in 70 to 80 percent of alcohol for 20 to 40 seconds or in 2 to 4 percent of sodium hypochlorite for 1 to 3 minutes, soaking the treated fresh dates in a preservative consisting of sodium nitroprusside, calcium chloride, tween 80 and water for 1 to 3 hours, fishing out the fresh dates, spreading in a thin and flat mode, air-drying, putting into a porous preservation bag, sealing, placing in a cool place, and storing at normal temperature. The method is low in preservation cost, and is economical, practical, safe and stable; during storage, the reddening rate and rotting rate of the fresh dates andthe content of soluble sugar and ethylene are reduced, and the content of vitamin c (Vc) and the activities of antioxidase, superoxide dismutase (SOD), catalase (CAT) and polyphenol oxidase (PPO) areimproved; and the storage period of the fresh dates is prolonged by 7 to 8 days.

The invention discloses lactobacillus fermentum JX306 with an antioxidant function and application thereof, and belongs to the technical field of bioengineering. The lactobacillus fermentum JX306 hasa high DPPH free radical and hydroxyl radical scavenging ability, high reducing power, a high Fe2+ chelating ability, a favorable anti-lipid peroxidation ability, and favorable bile salt tolerance, gastrointestinal fluid resistivity and hydrogen peroxide tolerance; and the glutathione peroxidase activity can be remarkably improved, the malondialdehyde level is reduced, the levels of five antioxidant enzymes such as TR3, Prdx1, Gsr, Gpx1 and a nuclear transcription factor Nrf2 are remarkably improved, and the D-gal induced histopathological lesion can be remarkably relieved. The lactobacillus fermentum JX306 provided by the invention plays a big role on improving the oxidation resistance of food with the lactobacillus fermentum JX306 as a fermenting microbe, and has a wide application prospect.

The present invention discloses a preparation method for a novel red blood cell substitute-artificial red blood cell fluorescent nanoparticles. According to the present invention, a cross-linking technology is adopted to carry out a cross-linking reaction for hemoglobin (Hb), catalase (CAT), superoxide dismutase (SOD) and carbonic anhydrase (CA) to generate a polyHb-SOD-CAT-CA complex body, wherein the cross-linking technology is performed by a bifunctional reagent glutaraldehyde; the polyHb-SOD-CAT-CA complex body is further embedded by polylactic acid and polyethylene glycol copolymer (PLA-PEG) nanoparticles. With the present invention, the antioxidase of the SOD and the CAT in the complex body can eliminate the oxygen free radicals generated during the blood transfusion process, such that the ischemia-reperfusion injury easily generated during the blood replacement process can be avoided; with the CA, the red blood cell substitute of the present invention has oxygen carrying performance and carbon dioxide transportation performance; with the PLA-PEG microcapsule embedding, the half life period of the artificial blood product can be prolonged, the biocompatibility of the artificial blood product can be increased.

The invention discloses a feed for improving the reduction of processing characteristics of eggs caused by vanadium. The feed comprises the following components: corn, soybean oil, soybean meal, calcium carbonate, dicalcium phosphate, table salt, choline chloride, composite multi vitamins, mineral premixes, DL- methionine and premixes. The invention further provides a premix for improving the reduction of processing characteristics of eggs caused by vanadium, and the premix comprises the following components: tea polyphenol, vitamin C, vitamin E, beta-carotene, lycopene, resveratrol, sodium selenite and rice chaff. The invention further provides a preparation method of the feed for improving the reduction of processing characteristics of eggs caused by vanadium. The premix can promote the antioxidant ability of the organism of a laying hen by improving the activity of antioxidase so as to reduce the oxidative damage of a fallopian tube induced by the vanadium and improve the reduction of the processing quality of the eggs caused by the vanadium.