358 results about "Antioxidative enzyme" patented technology

The invention discloses a method for efficiently screening saline-alkaline tolerant rice. According to the method, controllable indoor saline-alkali environment simulation conditions are constructed, various salts are mixed in proportion to prepare solutions with different salinity gradients, the pH value is adjusted, and temperature, humidity and illumination parameters are accurately controlled; the method comprises the following steps: disinfecting rice seeds, carrying out a germination test, transplanting rice seedlings, and regularly measuring growth indexes; in the treatment process, physiological indexes such as malondialdehyde content, antioxidant enzyme activity, proline content and the like and the expression level of salt and alkali tolerance related genes are detected, key indexes are screened by applying statistics and multivariate analysis methods, and an evaluation system is established for comprehensive score screening. The method can quickly perform saline-alkali stress treatment on a large number of rice seeds or seedlings, is not limited by natural environment, shortens the screening period, can accurately simulate different saline-alkali soil environments, scientifically evaluates the saline-alkali tolerance of rice from multiple dimensions, and provides an efficient and reliable technical means for breeding saline-alkali tolerant rice varieties.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention discloses a preparation method and application of yak blood peptide with anti-hypoxia and anti-fatigue functions. The method comprises the steps of raw material pretreatment, composite enzymolysis (adopting a mixture of enzyme digestion modified lumbrukinase and nattokinase), enzyme deactivation, separation and purification, ferrous chelation, Maillard reaction, concentration and drying and the like. The enzymolysis efficiency and the active peptide yield are remarkably improved through ultrasonic treatment and a composite enzymolysis technology, the stability and the anti-hypoxia capacity of peptide fragments are enhanced through ferrous chelation, and the antioxidant activity and the anti-fatigue activity are further improved through the Maillard reaction. Experiments show that the yak blood peptide prepared by the method can significantly improve the antioxidant enzyme activity, prolong the normal-pressure hypoxia survival time, prolong the swimming exhaustion time and significantly reduce the MDA content of brain tissue and myocardial tissue. The obtained product can be applied to anti-hypoxia and anti-fatigue medicines or health-care foods, and has the advantages of high process efficiency, remarkable activity, high safety and the like.

The invention provides application of enhanced and optimized mesenchymal stem cells and exosomes in anti-aging or senescence delaying, and belongs to the technical field of biological medicine manufacturing. According to the pharmaceutical composition disclosed by the invention, the activity-enhanced mesenchymal stem cells and the exosomes secreted by the activity-enhanced mesenchymal stem cells are applied in a combined manner, so that a remarkable synergistic interaction effect is shown in anti-aging intervention. Experiments prove that the composition can systematically improve oxidative stress injury related to aging, significantly recover antioxidant enzyme activity of serum and brain tissue to be close to a normal level, and effectively reverse cognitive function degeneration, which is represented as behavioral improvement such as shortening of escape latency, improvement of spatial memory ability and the like; the invention is suitable for preparing drugs or functional preparations for delaying body aging or resisting aging, and provides an innovative technical path for developing an efficient and safe anti-aging therapy.

The invention belongs to the technical field of biology, and particularly relates to application of serine in relieving heat stress of pleurotus ostreatus and a method. Under the heat stress condition, exogenous serine supply promotes pleurotus ostreatus hypha to recover growth, and the activity of antioxidant enzyme, the yield of sporocarp and the yield of nutrient substances are improved; when the activity of the PHGDH enzyme is inhibited, the serine synthesis is reduced; the hypha growth can be promoted by overexpressing the phgdh, and the enzymatic activity of the PHGDH can be improved; when acetyltransferases HAT, NAT1, NAT2 and NAT3 act on a K394 site of the PHGDH, the enzyme activity of the PHGDH can be improved, endogenous serine metabolism is activated to respond to heat stress, it is indicated that PHGDH-mediated serine metabolism plays an important role in corresponding heat stress of the pleurotus ostreatus, and the invention discloses a mechanism for improving heat resistance of the pleurotus ostreatus through the PHGDH-mediated serine metabolism. And a foundation can be laid for breeding high-temperature-resistant pleurotus ostreatus dominant strains.

The invention provides an application of a lactobacillus paracasei IOB413 metagen in prevention of helicobacter pylori infection, and relates to the technical field of microorganisms. The lactobacillus paracasei IOB413 metaplasm can competitively occupy gastric epithelial cell adhesion sites to directly block the initial colonization of helicobacter pylori, so that infection preposed interception is realized, and the lactobacillus paracasei IOB413 metaplasm is suitable for early prevention of high-incidence people. The compound has dual mechanisms of site blocking and urease inhibition, and can cooperate with antibiotics to improve the eradication rate and reduce the drug resistance. Animal experiments show that the traditional Chinese medicine composition can reduce gastric mucosa proinflammatory factors in a dose-dependent manner, improve the activity of antioxidant enzymes, reduce lipid peroxidation products, relieve mucosa lesion, promote repair and reduce the risk of complications; moreover, gastrointestinal hormone disorder can be reversed, the digestive function is maintained, and related discomfort is relieved.

The invention relates to the technical field of industrial microorganisms, and particularly discloses a P.megatherium HHY-1, a microbial agent thereof and application of the P.megatherium HHY-1. The preservation number of the P.megaterium HHY-1 is CGMCC (China General Microbiological Culture Collection Center) No.34470, and the preservation number of the P.megaterium HHY-1 is CGMCC No.34470. The P.megatherium provided by the invention has multiple functions of nitrogen fixation, phosphorus solubilization, siderophore production, IAA production capacity, antioxidant enzyme activity and the like, can play a comprehensive role from multiple dimensions of nutrition supply, growth regulation, stress resistance protection and the like, effectively assists good growth of the Abajie wheat 19 in a saline-alkali environment, and has a good application prospect. The method has great significance in improving the yield of the drought alkaline wheat in the saline-alkali soil and promoting agricultural sustainable development, and shows extremely high practical value in the aspect of growth promotion of the drought alkaline wheat Gie wheat 19.

The invention provides a chitosan-iron nanoparticle leaf surface resistance and control agent as well as a preparation method and application thereof, and belongs to the technical field of reduction of heavy metal pollution of crops. The preparation method comprises the following steps: dissolving chitosan in an organic solvent to obtain a chitosan solution, mixing the chitosan solution with ferrous sulfate, adding a sodium tripolyphosphate solution for crosslinking and stirring to obtain a solution containing precipitates, centrifuging, and collecting the precipitates to obtain the chitosan / ferrous sulfate composite material. The TPP and amino groups of the chitosan form ionic crosslinking through triphosphate groups, and Fe < 2 + > is successfully loaded on the surfaces of the chitosan nanoparticles. By complexing chitosan and Fe < 2 + >, the crystallinity of the material is reduced, and the adsorption activity of the amorphous structure is enhanced. Pot experiments show that by spraying the chitosan-iron nanoparticle leaf surface resistance and control agent on leaf surfaces, heavy metals of crops can be remarkably reduced, the antioxidant enzyme activity of plant tissues is improved, active oxygen is effectively removed, oxidative damage is relieved, and therefore plant growth is promoted, and the toxic effect of heavy metal stress on the crops is relieved.

The invention belongs to the technical field of medicines, and particularly relates to a plant lactobacillus for relieving acute alcoholic liver injury as well as a metagen and application of the plant lactobacillus. The strain of the plant lactobacillus L5-13 is preserved in the China Center for Type Culture Collection on August 18, 2025, and the preservation number of the strain is CCTCC NO: M 20251835. The strain and the metagen thereof can significantly improve pathological symptoms of mice with acute alcoholic liver injury, including: reducing the levels of ALT, AST, TC, TG and LDL-C in serum of the mice with acute alcoholic liver injury, and increasing the level of HDL-C; the abnormal blood lipid level of the mouse caused by acute alcohol is regulated; the activity of antioxidant enzymes in the liver is improved, and the lipid peroxidation level is reduced; the ethanol dehydrogenase activity of the liver is improved, and the ethanol metabolism capability is enhanced. Therefore, the strain and the metagen thereof have wide application prospects in prevention or treatment of acute alcoholic liver injury.

The invention relates to the field of microorganisms, and in particular relates to a Brucella intermedia 33, a vegetable preservative and application of the Brucella intermedia 33 and the vegetable preservative. The invention provides a strain of Buttiauxella agrestis 33, and the preservation number of the Buttiauxella agrestis 33 is CGMCC (China General Microbiological Culture Collection Center) No.34251. The invention also provides a preparation method of the Buttiauxella agrestis 33. After broccoli is treated by the strain, the postharvest yellowing of the broccoli can be obviously inhibited. Afterwards, the expression of genes related to chlorophyll metabolism pathways and antioxidant enzymes in the broccoli treated by utilizing the Brucella intermedia 33 is detected through real-time fluorescent quantitative reverse transcription PCR, and the result shows that the Brucella intermedia 33 inhibits yellowing of the broccoli after being picked by activating chlorophyll synthesis and the expression of the genes related to the antioxidant enzymes; and the oxidation resistance of the broccoli is improved.

The invention discloses a polypeptide fertilizer beneficial to cold resistance of crops and a preparation method thereof, and belongs to the technical field of agricultural fertilizers, the fertilizer is prepared by taking livestock and poultry blood (preferably pig blood) as a raw material, preparing high iron protein peptide through a compound protease hydrolysis process, and matching with a trace element compound, humic acid and a carrier. The preparation process comprises the steps of enzymolysis preparation of the ferritin peptide, fertilizer mixing, granulation, drying and the like. The polypeptide fertilizer disclosed by the invention takes the high-iron protein peptide as a core, the contained small-molecular amino acid polypeptide can enhance the antioxidant enzyme activity of crops at low temperature and reduce malonaldehyde accumulation, the porphyrin iron can promote chlorophyll synthesis and energy metabolism, and the high-iron protein peptide and the porphyrin iron cooperate to remarkably improve the cold resistance of the crops. Meanwhile, by cooperating with trace elements and humic acid, the cold resistance is enhanced, nutrient absorption of crops is promoted, the yield and quality are improved, multiple effects of resisting cold, improving quality, increasing yield and the like are achieved, the raw materials are wide in source, the cost is low, the preparation process is simple, and large-scale production and application are facilitated.

The invention discloses a ganoderma lucidum lipid exosome containing a liver protection component and a preparation method and application thereof.The preparation method comprises the steps that the liver protection component is wrapped with a coarsely extracted ganoderma lucidum exosome, then the liver protection component is wrapped with lipidosome, and therefore a lipidosome-exosome-liver protection component three-layer structure is formed from outside to inside; in the three-layer structure, the outer layer liposome can protect the storage of the liver protection component and can improve the transmembrane transport efficiency, the middle layer exosome serves as a carrier and can reach a designated position to be released, and the inner layer contains the liver protection component and can improve the transmembrane transport efficiency. Cell cycle arrest can be prevented, antioxidant enzyme activity can be improved, cell membranes can be protected from oxidative damage, and redox steady state can be maintained to relieve cell aging. The prepared ganoderma lucidum lipid exosome containing the liver protection component realizes slow release synergy through a three-layer structure, and the action time of the active component is remarkably prolonged.

The invention relates to an application of a FaPDHE1alpha gene or an encoding protein thereof in prevention and control of strawberry gray mold, and belongs to the field of plant genetic engineering and the field of prevention and control of postharvest diseases of fruits and vegetables. The invention discloses a key positive regulation and control effect of FaPDHE1alpha in regulation and control of strawberry gray mold resistance. By constructing a 35S:: FaPDHE1alpha overexpression vector and a FaPDHE1alpha RNAi interference vector, according to transient gene overexpression and RNA interference silencing tests, it is verified that high expression of the FaPDHE1alpha gene can obviously enhance the resistance of strawberry fruits to botrytis cinerea, and silencing of the gene causes reduction of the disease resistance of the fruits. Mechanism studies show that after the FaPDHE1alpha gene is activated, mitochondrial energy metabolism and controlled ROS signal generation are promoted, antioxidant enzyme activity is enhanced, and defense related gene expression is induced, so that the disease resistance of fruits is improved. By means of functional genomics, the key function of the FaPDHE1alpha gene in prevention and control of gray mold after strawberry harvest is illuminated for the first time, and the FaPDHE1alpha gene can be used for screening disease-resistant breeding materials or developing biological prevention and control preparations for postharvest diseases and has important application value.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a GhHAT18 gene in regulation and control of drought stress resistance of plants, the nucleotide sequence of the GhHAT18 gene is shown as SEQ ID NO.1. The invention discloses that the cotton histone acetylase gene GhHAT18 can be used for regulating and controlling the drought resistance of the plants for the first time, and experiments show that compared with a wild type plant, after the expression of the GhHAT18 gene is inhibited, the GhHAT18 gene can be used for regulating and controlling the drought stress resistance of the plants. The antioxidant enzyme activity and the drought resistance of plants can be obviously reduced; and the transgenic plant over-expressed with the GhHAT18 shows stronger and higher antioxidant enzyme activity and drought resistance under the drought stress. Transcriptome analysis shows that the GhHAT18 gene affects the expression of drought-resistant related genes through a secondary metabolite synthesis pathway and an MAPK signal transduction pathway, and the adaptive capacity of plants to drought stress is further regulated and controlled.

The invention discloses a preparation method and application of cubilose peptide. The amino acid sequence of the cubilose peptide is KPAPPKPEPK. The cubilose peptide prepared by the invention has strong ultraviolet damage resistance, and can improve the cell survival rate, improve the antioxidant enzyme level in serum, scavenge free radicals and reduce the inflammatory factor level in skin tissues. When the cubilose peptide is used for developing anti-ultraviolet damage products, the efficacy of cubilose can be deeply excavated, and the quality and economic value of cubilose products are improved.

The present invention discloses a stress-resistant and growth-promoting microbial agent and its preparation method and application, which belong to the field of microbial technology. The stress-resistant and growth-promoting microbial agent of the present invention comprises Bacillus siamese, Burkholderia bivariate, and Sphingomonas yunnanensis. After the bacterial liquid of the three strains is mixed in equal proportions, they cooperate with each other to achieve significant stress-resistant and growth-promoting effects, especially in improving stress resistance indicators during the growth process of wheat plants, which is manifested in increasing the activity of the antioxidant enzyme SOD in the plants, increasing the content of the osmotic regulating substance proline, and reducing the content of MDA. The microbial agent of the present invention, when applied in conjunction with conventional fertilization, significantly promotes the growth of wheat seedlings under salt stress and increases wheat yield.

Provided herein are compositions, systems, kits, and methods for treating a patient with a thromboembolism by administering nanoconjugates comprising nanoparticles encapsulating and / or conjugated to: i) tissue-type plasminogen activator (tPA), and ii) at least one antioxidant enzyme selected from the group consisting of: superoxide dismutase, glutathione peroxidase, glutathione reductase, and a catalase.

The invention discloses application of an exogenous regulating substance in relieving corn drought stress, and belongs to the technical field of biology. It is verified that external application of serine, pantothenic acid or raffinose can promote root morphology establishment of corn under drought stress, enhance antioxidant enzyme activity of corn roots, reduce MDA accumulation and increase soluble protein content, 0.6 mM of serine, 0.9 mM of pantothenic acid and 1.0 mM of raffinose can effectively relieve stress of the drought environment on corn, and the corn drought resistance can be effectively improved. The water retention capacity of corn is improved, the antioxidant enzyme activity of corn roots is enhanced, the content of osmotic regulation substances is regulated and controlled, damage of drought stress to corn cells is reduced, and meanwhile growth of corn plants and establishment of root morphology are promoted. The invention provides a new strategy for drought-resistant cultivation of corn.

The invention discloses a CabHLH143 gene and application of the CabHLH143 gene in regulation and control of salt tolerance of pepper plants. The nucleotide sequence of the CabHLH143 gene is as shown in SEQ ID NO: 1, a bHLH transcription factor with a salt stress response function is coded, and the amino acid sequence is as shown in SEQ ID NO: 2. By constructing a recombinant expression vector and introducing the recombinant expression vector into a pepper plant, it is found that overexpression of the CabHLH143 can significantly improve the tolerance of the plant to salt stress, which is expressed by regulating the ratio of Na < + > / K < + >, enhancing the activity of antioxidant enzyme, promoting accumulation of proline and reducing the levels of MDA and ROS. The invention further provides a recombinant vector containing the CabHLH143 gene, a host cell, a transgenic plant, a specific primer and a detection kit, and the recombinant vector, the host cell, the transgenic plant, the specific primer and the detection kit are suitable for plant molecular breeding, gene function research and salt-tolerant variety screening and have wide application prospects.

The invention discloses a whitening antioxidant composition containing a citrus chachiensis hortorum extract. The whitening antioxidant composition is prepared from the citrus chachiensis hortorum extract, ergothioneine and PDRN. The weight ratio of the citrus chachiensis hortorum extract to the ergothioneine to the PDRN is (0.1 to 5): (0.001 to 0.1): (0.01 to 1). Through ternary compounding of the citrus chachiensis hortorum extract, the ergothioneine and the PDRN, the synergistic effect of'inhibiting melanogenesis, removing ROS (reactive oxygen species) and improving antioxidant enzyme activity 'is realized, the expression of melanin precursor protein Pmel17 is inhibited, the levels of NNT and GSH-Px1 are improved, the content of reactive oxygen species (ROS) is reduced, and the problem of single effect in the prior art is solved. The composition has excellent whitening and anti-oxidation effects, is high in stability, good in safety and wide in application range, and can be widely applied to functional cosmetics such as whitening essence, face cream, emulsion and sunscreen cream.

The present invention belongs to the field of genetic engineering and specifically provides a protein PeuENH1 for improving plant salt tolerance. The amino acid sequence of the protein PeuENH1 is shown in SEQ ID NO.2. The protein PeuENH1 can improve plant tolerance to salt stress, reduce plant sensitivity to salt stress, participate in plant response to salt stress, reduce reactive oxygen content, H2O2 content and malondialdehyde content in transgenic plants under salt stress, increase proline content in transgenic plants, enhance the antioxidant enzyme system, reduce oxidative damage to the membrane system by reactive oxygen, thereby improving plant tolerance to salt stress. The protein has important theoretical and practical significance for improving saline-alkali land environment, increasing saline-alkali land utilization efficiency and expanding saline-alkali land vegetation coverage area.

The invention discloses application of a blueberry VcETR-1 gene in regulation of high temperature stress resistance of plants, and relates to the technical field of plant genetic engineering. According to the application of the blueberry VcETR-1 gene disclosed by the invention in regulating and controlling the high temperature stress resistance of the plant, the nucleotide sequence of the blueberry VcETR-1 gene is as shown in SEQ ID NO. 1. The gene can improve the stress resistance of a plant at a high temperature of 45 DEG C through overexpression, and can regulate the response of the plant to 200 [mu] mol / L of exogenous salicylic acid. Experiments show that 10-20% of leaves of the transgenic plant are still good in state after high-temperature treatment, and physiological indexes such as antioxidant enzyme activity and the like are better. The invention further provides a related application method and a primer pair for cloning the gene, the blank of research on the heat-resistant function of the blueberry ethylene receptor gene is filled, and an important basis and technical support are provided for cultivating new varieties of high-temperature-resistant plants.

The present application relates to the field of microorganism, and particularly to a strain of Buttiauxella agrestis 33, a vegetable fresh-keeping agent and application thereof.The present application provides a strain of Buttiauxella agrestis 33, and the preservation number of the strain is CGMCC No.34251.After the strain is used to treat green flower, the yellowing of the green flower after harvesting can be significantly inhibited.Then, the expression of genes related to chlorophyll metabolic pathway and antioxidant enzyme in the green flower treated by the Buttiauxella agrestis 33 is detected by real-time fluorescent quantitative reverse transcription PCR, and the result shows that the Buttiauxella agrestis 33 can inhibit the yellowing of the green flower after harvesting and improve the antioxidant capacity of the green flower by activating the expression of genes related to chlorophyll synthesis and antioxidant enzyme.

The invention belongs to the technical field of biology, and relates to application of an existing strain streptomyces rochei D74 in delaying leaf senescence. According to the invention, the antioxidant enzyme activity in the later growth and development period of tomato leaves can be improved, the photosynthesis intensity and the redox steady state are maintained, and yellowing and aging of the tomato leaves are delayed.

The invention relates to the technical field of nano-materials, and discloses a heterogeneous diatomic nano-enzyme as well as a preparation method and application thereof. The invention provides a space confinement regulation and control strategy which is used for constructing a heterodiatomic catalytic center on a nitrogen-doped carbon skeleton and preparing nano-enzyme with structural stability and synergistic catalytic performance. According to the method, a confinement framework formed by self-assembly of first metal ions such as cobalt ions and second metal ions such as zinc ions and organic ligands is used as a template, third metal ions such as iron ions are introduced, and then carbonization heat treatment is performed in a protective gas atmosphere, so that bimetal sites with stable bridging configuration are formed in situ. The obtained nano-enzyme has the catalytic activity of various antioxidant enzymes, including catalase and remarkable superoxide dismutase activity, and has more excellent free radical scavenging capacity compared with a single-metal or non-bridging system.

The present application relates to the field of biological medicine and modernization of traditional Chinese medicine, and particularly relates to a therapeutic target, an inhibitor and a traditional Chinese medicine composition for treating ovarian hypofunction caused by oxidative stress and application thereof. The present application first determines SRC tyrosine kinase as a key drug target for preventing and treating the disease. The application of the SRC tyrosine kinase inhibitor (such as secaitinib) in preparing related drugs is provided, which can alleviate the damage of ovarian granulosa cells by inhibiting the SRC activity, down-regulating the expression of antioxidant enzyme related genes and reducing the level of active oxygen. Meanwhile, the present application provides a traditional Chinese medicine composition composed of mulberry, kudzu root, tuckahoe and medlar. The composition can improve the hormone level, enhance the antioxidant capacity, improve the ovarian function and fertility by inhibiting the SRC tyrosine kinase activity and the SRC-RAF1-MEK-ERK signal pathway. The present application provides a new solution for the targeted treatment of ovarian hypofunction and the modernization of traditional Chinese medicine.

The application provides an engineered probiotic with intracellular chimeric nanoscale enzyme, which is a probiotic modified with several antioxidant enzymes on an inner membrane; the antioxidant enzyme is composed of a metal oxide nanoscale enzyme with a particle size less than 10 nm and a targeting molecular structure and a saccharide molecular structure modified on the surface of the metal oxide nanoscale enzyme; the targeting molecular structure and the saccharide molecular structure are connected to the surface of the metal oxide nanoscale enzyme through a connecting group containing a metal ligand structure based on coordination and electrostatic interaction, and the antioxidant enzyme accounts for 5-20% of the mass of the engineered probiotic. The application also provides a method for preparing the engineered probiotic and application of the engineered probiotic in preparation of a medicine or food for preventing or treating antibiotic-associated diarrhea. The engineered probiotic can realize efficient elimination of hydroxyl radicals near a membrane induced by an antibiotic, so as to accurately inhibit a lipid peroxidation reaction and protect the integrity of a cell membrane.

The invention belongs to the technical field of gene engineering, and particularly relates to a larch drought response gene LgLEA10, a protein and application thereof. The nucleotide sequence of the larch drought response gene LgLEA10 provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the protein expressed by the larch drought response gene LgLEA10 is as shown in SEQ ID NO.2. It is proved for the first time that the LgLEA10 gene of dahurian larch is a drought response regulation gene of dahurian larch. Tests prove that overexpression of the LgLEA10 gene can improve the drought resistance of larch by remarkably improving the activity of antioxidant enzyme and osmotic adjusting substances. The discovery provides important gene resources and theoretical basis for cultivating a new variety of larch with strong drought resistance.