427 results about "Antioxidative enzyme" patented technology

The invention relates to a Chinese wildrye rhizosphere growth-promoting bacterium and application of the Chinese wildrye rhizosphere growth-promoting bacterium in growth promotion and drought resistance of Chinese wildrye, and belongs to the technical field of microorganisms, the strain is classified and named as Paraburkholderia graminis, the strain number is B24, and the preservation number is CGMCC No.31907. The strain provided by the invention is derived from the intergrassland soil of leymus chinensis in an excessive grazing grassland, has good growth promoting characteristics of producing IAA, producing ACC deaminase, fixing nitrogen, dissolving phosphorus and the like, and also can obviously enhance the drought resistance of the leymus chinensis. When the strain is inoculated to the rhizosphere of a leymus chinensis seedling, the growth of the leymus chinensis can be obviously promoted, and the overground and underground biomass of the leymus chinensis is increased; the activity of antioxidant enzymes is reduced and the hormone content in leymus chinensis leaves is changed, so that drought stress is relieved, the photosynthesis of leymus chinensis is enhanced, and the drought resistance of leymus chinensis is remarkably improved. Therefore, the Burkholderia parakholderia can be used for development of a microbial fertilizer for grassland plants and applied to a drought stress habitat, so that the drought resistance of the grassland plant leymus chinensis is improved, and grassland management is optimized.

The invention discloses a method for efficiently screening saline-alkaline tolerant rice. According to the method, controllable indoor saline-alkali environment simulation conditions are constructed, various salts are mixed in proportion to prepare solutions with different salinity gradients, the pH value is adjusted, and temperature, humidity and illumination parameters are accurately controlled; the method comprises the following steps: disinfecting rice seeds, carrying out a germination test, transplanting rice seedlings, and regularly measuring growth indexes; in the treatment process, physiological indexes such as malondialdehyde content, antioxidant enzyme activity, proline content and the like and the expression level of salt and alkali tolerance related genes are detected, key indexes are screened by applying statistics and multivariate analysis methods, and an evaluation system is established for comprehensive score screening. The method can quickly perform saline-alkali stress treatment on a large number of rice seeds or seedlings, is not limited by natural environment, shortens the screening period, can accurately simulate different saline-alkali soil environments, scientifically evaluates the saline-alkali tolerance of rice from multiple dimensions, and provides an efficient and reliable technical means for breeding saline-alkali tolerant rice varieties.

The invention belongs to the technical field of gene engineering, and relates to a cyperus esculentus CeMYB154 gene capable of improving salt tolerance and active oxygen scavenging capacity and application of the cyperus esculentus CeMYB154 gene. The Cyperus esculentus CeMYB154 gene belongs to an MYB transcription factor family, the Cyperus esculentus CeMYB154 gene is screened and transformed into Arabidopsis thaliana through transcriptome sequencing, bioinformatics analysis, gene cloning, overexpression vector construction and recombinant strain overexpression, and analysis finds that under salt stress, compared with a wild type, the root length of a plant with the overexpressed CeMYB154 gene is obviously increased, the growth vigor is better, and the growth rate of the plant with the overexpressed CeMYB154 gene is higher. According to the present invention, the content of MDA and H2O2 is significantly reduced, and the activity of CAT, POD and SOD antioxidant enzymes is significantly enhanced, such that the overexpressed CeMYB154 gene can enhance the salt tolerance and the active oxygen scavenging ability of the plant, and the overexpressed CeMYB154 gene is proved to be the salt tolerance positive regulation factor; the CeMYB154 gene can be used in the field of salt-tolerant stress-resistant breeding of cyperus esculentus, and provides an important gene resource for salt-tolerant molecular breeding.

The invention provides an antioxidant enzyme-containing composition and a preparation method thereof, and relates to the technical field of enteritis treatment. The preparation method comprises the following steps: S1, collecting various component materials for preparing the antioxidant enzyme-containing composition: collecting various raw materials for preparing glucan, fructo-oligosaccharide, galactooligosaccharide, superoxide dismutase, vitamin C, bunge cherry seed powder, cassia seed powder, turmeric, sorbitol, hydroxypropyl methyl cellulose, silicon dioxide and magnesium stearate; according to the invention, a matched powder bagging machine is adopted to realize full-automatic unattended operation to automatically complete the large bag filling and sealing step in the steps S4-S10, vacuumizing is performed firstly and then sealing is performed, and the storage space is compressed by vacuumizing, so that the condition that the bag is expanded and broken by internal air pressure in the transfer and storage process is not easy to cause; in the bag sealing process, the phenomena of high hot melting efficiency and high sealing speed are achieved by adopting a bag strip guiding and rolling hot melting mode.

The invention relates to a method for improving cadmium stress resistance of rice by using carbon quantum dots (CDs), which solves the technical problem that rice growth is affected due to the fact that rice is deeply poisoned by cadmium in the prior art by spraying the carbon quantum dots on leaf surfaces of the rice or irrigating the carbon quantum dots into root soil of the rice. CDs are used as a leaf surface resistance and control agent to treat cadmium-stressed rice, and it is found that on the premise that growth and development of the rice are not affected, CDs can improve the stress resistance of the rice by promoting photosynthesis of the rice and can stimulate a rice defense system to generate more antioxidant enzymes to cope with oxidative damage caused by Cd stress. Besides, CDs can also reduce the concentration of DTPA-Cd in soil and reduce accumulation of Cd on the overground part so as to further reduce the toxicity of Cd to rice, and meanwhile, the Cd content of the overground part of the rice is reduced so as to be beneficial to reducing accumulation of Cd in grains, so that CDs can be used as a leaf surface resistance and control agent to effectively relieve cadmium toxic rice, thereby promoting growth and development of the rice and improving the yield.

The invention discloses an application of an msa-miR156e gene regulated by NO in regulation of drought resistance of plants, and is characterized in that a mature sequence of the msa-miR156e gene is as shown in SEQ ID No. 1, and a precursor sequence of the msa-miR156e gene is as shown in SEQ ID No. 2. The invention also discloses an application of the msa-miR156e gene in regulation of drought resistance of plants. According to the application, the mechanism of NO for regulating the alfalfa msa-miR156e gene to cope with drought stress is studied, the NO is found to up-regulate the expression of msa-miR156e, enhance the activity of antioxidant enzyme and reduce the accumulation of active oxygen, so that the drought resistance of alfalfa is remarkably improved, SPL family genes are regulated in a targeted manner through msa-miR156e, the physiological adaptability of plants is promoted, and the theoretical basis of plant stress resistance is enriched; the cultivation of a high-quality alfalfa variety adapting to a drought environment is facilitated.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention discloses a preparation method and application of yak blood peptide with anti-hypoxia and anti-fatigue functions. The method comprises the steps of raw material pretreatment, composite enzymolysis (adopting a mixture of enzyme digestion modified lumbrukinase and nattokinase), enzyme deactivation, separation and purification, ferrous chelation, Maillard reaction, concentration and drying and the like. The enzymolysis efficiency and the active peptide yield are remarkably improved through ultrasonic treatment and a composite enzymolysis technology, the stability and the anti-hypoxia capacity of peptide fragments are enhanced through ferrous chelation, and the antioxidant activity and the anti-fatigue activity are further improved through the Maillard reaction. Experiments show that the yak blood peptide prepared by the method can significantly improve the antioxidant enzyme activity, prolong the normal-pressure hypoxia survival time, prolong the swimming exhaustion time and significantly reduce the MDA content of brain tissue and myocardial tissue. The obtained product can be applied to anti-hypoxia and anti-fatigue medicines or health-care foods, and has the advantages of high process efficiency, remarkable activity, high safety and the like.

The invention provides application of enhanced and optimized mesenchymal stem cells and exosomes in anti-aging or senescence delaying, and belongs to the technical field of biological medicine manufacturing. According to the pharmaceutical composition disclosed by the invention, the activity-enhanced mesenchymal stem cells and the exosomes secreted by the activity-enhanced mesenchymal stem cells are applied in a combined manner, so that a remarkable synergistic interaction effect is shown in anti-aging intervention. Experiments prove that the composition can systematically improve oxidative stress injury related to aging, significantly recover antioxidant enzyme activity of serum and brain tissue to be close to a normal level, and effectively reverse cognitive function degeneration, which is represented as behavioral improvement such as shortening of escape latency, improvement of spatial memory ability and the like; the invention is suitable for preparing drugs or functional preparations for delaying body aging or resisting aging, and provides an innovative technical path for developing an efficient and safe anti-aging therapy.

The invention discloses a method for improving drought tolerance and yield of wheat through combined application of compost and sodium selenite, and belongs to the technical field of agricultural planting. According to the method, wheat is planted after compost is applied to soil, and when drought stress occurs, a sodium selenite solution is sprayed to wheat leaves, so that oxidative stress indexes such as MDA, H2O2 and O2 <-> of wheat can be remarkably reduced, and the activity of antioxidant enzymes such as SOD, POD, CAT and APX can be improved; physiological indexes such as chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, protein, soluble sugar and proline of wheat leaves are improved, growth and yield related traits of the wheat are improved, and finally the drought resistance and yield of the wheat are improved. The compost is prepared by taking 50%-55% of corn straw, 10%-20% of flue-cured tobacco straw, 20%-25% of rice straw, 10%-15% of livestock manure and 0.1%-1% of bacillus subtilis as raw materials, adding organic wastewater or tap water, mixing, composting and fermenting.

The invention belongs to the technical field of biology, and particularly relates to application of serine in relieving heat stress of pleurotus ostreatus and a method. Under the heat stress condition, exogenous serine supply promotes pleurotus ostreatus hypha to recover growth, and the activity of antioxidant enzyme, the yield of sporocarp and the yield of nutrient substances are improved; when the activity of the PHGDH enzyme is inhibited, the serine synthesis is reduced; the hypha growth can be promoted by overexpressing the phgdh, and the enzymatic activity of the PHGDH can be improved; when acetyltransferases HAT, NAT1, NAT2 and NAT3 act on a K394 site of the PHGDH, the enzyme activity of the PHGDH can be improved, endogenous serine metabolism is activated to respond to heat stress, it is indicated that PHGDH-mediated serine metabolism plays an important role in corresponding heat stress of the pleurotus ostreatus, and the invention discloses a mechanism for improving heat resistance of the pleurotus ostreatus through the PHGDH-mediated serine metabolism. And a foundation can be laid for breeding high-temperature-resistant pleurotus ostreatus dominant strains.

The invention provides an application of a lactobacillus paracasei IOB413 metagen in prevention of helicobacter pylori infection, and relates to the technical field of microorganisms. The lactobacillus paracasei IOB413 metaplasm can competitively occupy gastric epithelial cell adhesion sites to directly block the initial colonization of helicobacter pylori, so that infection preposed interception is realized, and the lactobacillus paracasei IOB413 metaplasm is suitable for early prevention of high-incidence people. The compound has dual mechanisms of site blocking and urease inhibition, and can cooperate with antibiotics to improve the eradication rate and reduce the drug resistance. Animal experiments show that the traditional Chinese medicine composition can reduce gastric mucosa proinflammatory factors in a dose-dependent manner, improve the activity of antioxidant enzymes, reduce lipid peroxidation products, relieve mucosa lesion, promote repair and reduce the risk of complications; moreover, gastrointestinal hormone disorder can be reversed, the digestive function is maintained, and related discomfort is relieved.

The invention relates to the technical field of industrial microorganisms, and particularly discloses a P.megatherium HHY-1, a microbial agent thereof and application of the P.megatherium HHY-1. The preservation number of the P.megaterium HHY-1 is CGMCC (China General Microbiological Culture Collection Center) No.34470, and the preservation number of the P.megaterium HHY-1 is CGMCC No.34470. The P.megatherium provided by the invention has multiple functions of nitrogen fixation, phosphorus solubilization, siderophore production, IAA production capacity, antioxidant enzyme activity and the like, can play a comprehensive role from multiple dimensions of nutrition supply, growth regulation, stress resistance protection and the like, effectively assists good growth of the Abajie wheat 19 in a saline-alkali environment, and has a good application prospect. The method has great significance in improving the yield of the drought alkaline wheat in the saline-alkali soil and promoting agricultural sustainable development, and shows extremely high practical value in the aspect of growth promotion of the drought alkaline wheat Gie wheat 19.

The invention provides a chitosan-iron nanoparticle leaf surface resistance and control agent as well as a preparation method and application thereof, and belongs to the technical field of reduction of heavy metal pollution of crops. The preparation method comprises the following steps: dissolving chitosan in an organic solvent to obtain a chitosan solution, mixing the chitosan solution with ferrous sulfate, adding a sodium tripolyphosphate solution for crosslinking and stirring to obtain a solution containing precipitates, centrifuging, and collecting the precipitates to obtain the chitosan / ferrous sulfate composite material. The TPP and amino groups of the chitosan form ionic crosslinking through triphosphate groups, and Fe < 2 + > is successfully loaded on the surfaces of the chitosan nanoparticles. By complexing chitosan and Fe < 2 + >, the crystallinity of the material is reduced, and the adsorption activity of the amorphous structure is enhanced. Pot experiments show that by spraying the chitosan-iron nanoparticle leaf surface resistance and control agent on leaf surfaces, heavy metals of crops can be remarkably reduced, the antioxidant enzyme activity of plant tissues is improved, active oxygen is effectively removed, oxidative damage is relieved, and therefore plant growth is promoted, and the toxic effect of heavy metal stress on the crops is relieved.

The invention relates to the technical field of crop planting, and provides a method for promoting germination of brassica napus seeds under low-temperature stress by using allantoin. The method comprises the following steps: firstly, disinfecting cabbage type rape seeds, and then soaking the cabbage type rape seeds in an allantoin solution with a specific concentration under room-temperature and weak-light conditions; the treated seeds do not need to be washed and are directly placed in a culture dish paved with double-layer filter paper, a proper amount of distilled water is added, and then the seeds are cultured in an incubator simulating a low-temperature environment. Experimental results show that the germination rate, the germination index and the vitality index of the brassica napus seeds are remarkably improved through allantoin treatment, and the average germination time is shortened. Mechanism research finds that allantoin improves the low-temperature resistance of the brassica napus by various ways of increasing the content of free water of seedlings, increasing the content of soluble sugar and proline, enhancing the activity of antioxidant enzyme, reducing active oxygen and malonaldehyde accumulation and the like. The method disclosed by the invention is low in cost and environment-friendly, and provides powerful guarantee for field production such as late direct seeding of the brassica napus.

The invention belongs to the technical field of medicines, and particularly relates to a plant lactobacillus for relieving acute alcoholic liver injury as well as a metagen and application of the plant lactobacillus. The strain of the plant lactobacillus L5-13 is preserved in the China Center for Type Culture Collection on August 18, 2025, and the preservation number of the strain is CCTCC NO: M 20251835. The strain and the metagen thereof can significantly improve pathological symptoms of mice with acute alcoholic liver injury, including: reducing the levels of ALT, AST, TC, TG and LDL-C in serum of the mice with acute alcoholic liver injury, and increasing the level of HDL-C; the abnormal blood lipid level of the mouse caused by acute alcohol is regulated; the activity of antioxidant enzymes in the liver is improved, and the lipid peroxidation level is reduced; the ethanol dehydrogenase activity of the liver is improved, and the ethanol metabolism capability is enhanced. Therefore, the strain and the metagen thereof have wide application prospects in prevention or treatment of acute alcoholic liver injury.

The invention discloses application of exogenous selenium to improvement of tomato late blight resistance, and belongs to the field of plant disease control. The exogenous selenium comprises Na2SeO3 (sodium selenite). Compared with a control group, exogenous selenium can inhibit the morbidity of tomato plants. Specifically, the exogenous selenium can inhibit the expansion of tomato leaf late blight scabs, relieve the accumulation of H2O2 and O2 <-> in the leaves and reduce the ROS content in the tomato leaves; meanwhile, the activity of antioxidant enzymes SOD, POD and CAT and the content of chlorophyll can be improved, and generation of MDA is reduced; besides, the content of SA and JA of tomato plants and the content of selenium in tomato leaves and fruits can also be increased by spraying exogenous selenium; in addition, through detection, exogenous selenium can improve the resistance of tomatoes to phytophthora infestans by inducing expression of the SIPR5 gene. In conclusion, the invention finds that the exogenous selenium can improve the resistance of the tomato to the late blight, and provides a new method for preventing and treating the tomato late blight.

The invention provides a microalgae fertilizer capable of improving salt and alkali resistance of cucumbers, and the microalgae fertilizer is a composite microalgae bio-fertilizer and comprises scenedesmus obliquus, nitzschia grain and extracellular polymeric substances generated in the culture process of the nitzschia grain. The components of the microalgae compound fertilizer disclosed by the invention are all freshwater algae strains and natural active microalgae cells, so that sodium ions and metal cations cannot be accumulated, the microalgae compound fertilizer does not contain blue-green algae, and cytotoxin which is harmful to the environment cannot be generated; the used algae strains are easier to breed, and the biomass accumulation speed of the algae strains is far higher than that of other algae strains, so that the prepared microalgae biological fertilizer has the advantages of saving time and cost. And the polyunsaturated fatty acid generated by the microalgae fertilizer can enhance the plant resistance through an osmotic regulation system and an antioxidant enzyme ROS system.

The invention relates to a plant growth-promoting rhizobacteria. The preservation number of the plant growth-promoting rhizobacteria is GDMCC No: 65494. Through rhizosphere soil microorganism sampling of Gannan navel oranges, the strain G41 with the characteristics of producing indoleacetic acid, producing siderophores and solubilizing phosphorus is successfully separated out. Furthermore, pot experiments verify that the strain group is inoculated, so that the plant height, stem diameter, biomass, chlorophyll content and antioxidant enzyme activity of navel orange seedlings are increased, and the strain has an excellent growth promoting effect. Through strain identification, the strain is formally named as the bacillus subtilis G41, and the bacillus subtilis G41 is named as the bacillus subtilis G41. In particular, it is worth mentioning that the strain has rich gene resources, 32 genes related to growth promoting characteristics are excavated from the gene level, and the genes are widely distributed in key processes such as indoleacetic acid biosynthesis, siderophore biosynthesis and transport and phosphorus circulation.

The invention relates to a specially selected strain and application thereof in cardiovascular and cerebrovascular maintenance. The invention provides a specially selected strain-prebiotic composition for enhancing and maintaining an ozone high-dose blood purification effect and application of the specially selected strain-prebiotic composition in establishment of an intestinal barrier function and health maintenance of heart and cerebral vessels. According to the invention, blood cell metabolism, immune system and antioxidant enzyme system can be activated by autologous blood circulation reinfusion, the state of the blood vessel wall is improved, and the physiological function of blood cells is enhanced; the specially selected targeted strain-prebiotic composition can regulate intestinal flora balance, establish an intestinal barrier function, maintain and accelerate metabolism of a human body, decompose blood fat and toxin garbage accumulated in the body, promote detoxification of the intestinal tract, purify the intestinal tract and reduce intestinal toxin substances entering the blood circulation of the human body through a portal system. According to the combination scheme, the blood purification effect can be remarkably improved, the blood fat index is remarkably improved, the purposes of recovering the blood vessel function and enhancing the human immune function are jointly achieved, a new technical scheme and means are provided for prevention and treatment of cardiovascular and cerebrovascular diseases, and wide application prospects are achieved.

The invention relates to the field of microorganisms, and in particular relates to a Brucella intermedia 33, a vegetable preservative and application of the Brucella intermedia 33 and the vegetable preservative. The invention provides a strain of Buttiauxella agrestis 33, and the preservation number of the Buttiauxella agrestis 33 is CGMCC (China General Microbiological Culture Collection Center) No.34251. The invention also provides a preparation method of the Buttiauxella agrestis 33. After broccoli is treated by the strain, the postharvest yellowing of the broccoli can be obviously inhibited. Afterwards, the expression of genes related to chlorophyll metabolism pathways and antioxidant enzymes in the broccoli treated by utilizing the Brucella intermedia 33 is detected through real-time fluorescent quantitative reverse transcription PCR, and the result shows that the Brucella intermedia 33 inhibits yellowing of the broccoli after being picked by activating chlorophyll synthesis and the expression of the genes related to the antioxidant enzymes; and the oxidation resistance of the broccoli is improved.

The invention provides application of fermented lactobacillus mucus IOB802 and a metagen thereof in improving eye health, and relates to the technical field of microbial fermentation. The strain improves the activity of retinal epithelial cells so as to enhance the antioxidant capacity, provide metabolic support, promote cell repair, maintain blood-retinal barrier, reduce apoptosis and other mechanisms, reduce the content of proinflammatory factors, reduce the content of vascular endothelial growth factors and reduce the expression level of inflammatory factors. The antioxidant enzyme activity and macular region edema can be improved, and the eye health can be improved.

The invention discloses a polypeptide fertilizer beneficial to cold resistance of crops and a preparation method thereof, and belongs to the technical field of agricultural fertilizers, the fertilizer is prepared by taking livestock and poultry blood (preferably pig blood) as a raw material, preparing high iron protein peptide through a compound protease hydrolysis process, and matching with a trace element compound, humic acid and a carrier. The preparation process comprises the steps of enzymolysis preparation of the ferritin peptide, fertilizer mixing, granulation, drying and the like. The polypeptide fertilizer disclosed by the invention takes the high-iron protein peptide as a core, the contained small-molecular amino acid polypeptide can enhance the antioxidant enzyme activity of crops at low temperature and reduce malonaldehyde accumulation, the porphyrin iron can promote chlorophyll synthesis and energy metabolism, and the high-iron protein peptide and the porphyrin iron cooperate to remarkably improve the cold resistance of the crops. Meanwhile, by cooperating with trace elements and humic acid, the cold resistance is enhanced, nutrient absorption of crops is promoted, the yield and quality are improved, multiple effects of resisting cold, improving quality, increasing yield and the like are achieved, the raw materials are wide in source, the cost is low, the preparation process is simple, and large-scale production and application are facilitated.

The invention discloses a ganoderma lucidum lipid exosome containing a liver protection component and a preparation method and application thereof.The preparation method comprises the steps that the liver protection component is wrapped with a coarsely extracted ganoderma lucidum exosome, then the liver protection component is wrapped with lipidosome, and therefore a lipidosome-exosome-liver protection component three-layer structure is formed from outside to inside; in the three-layer structure, the outer layer liposome can protect the storage of the liver protection component and can improve the transmembrane transport efficiency, the middle layer exosome serves as a carrier and can reach a designated position to be released, and the inner layer contains the liver protection component and can improve the transmembrane transport efficiency. Cell cycle arrest can be prevented, antioxidant enzyme activity can be improved, cell membranes can be protected from oxidative damage, and redox steady state can be maintained to relieve cell aging. The prepared ganoderma lucidum lipid exosome containing the liver protection component realizes slow release synergy through a three-layer structure, and the action time of the active component is remarkably prolonged.

The invention relates to an application of a FaPDHE1alpha gene or an encoding protein thereof in prevention and control of strawberry gray mold, and belongs to the field of plant genetic engineering and the field of prevention and control of postharvest diseases of fruits and vegetables. The invention discloses a key positive regulation and control effect of FaPDHE1alpha in regulation and control of strawberry gray mold resistance. By constructing a 35S:: FaPDHE1alpha overexpression vector and a FaPDHE1alpha RNAi interference vector, according to transient gene overexpression and RNA interference silencing tests, it is verified that high expression of the FaPDHE1alpha gene can obviously enhance the resistance of strawberry fruits to botrytis cinerea, and silencing of the gene causes reduction of the disease resistance of the fruits. Mechanism studies show that after the FaPDHE1alpha gene is activated, mitochondrial energy metabolism and controlled ROS signal generation are promoted, antioxidant enzyme activity is enhanced, and defense related gene expression is induced, so that the disease resistance of fruits is improved. By means of functional genomics, the key function of the FaPDHE1alpha gene in prevention and control of gray mold after strawberry harvest is illuminated for the first time, and the FaPDHE1alpha gene can be used for screening disease-resistant breeding materials or developing biological prevention and control preparations for postharvest diseases and has important application value.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

The invention discloses a seed treatment method for improving seed germination and planting efficiency of medicago sativa under drought conditions, and belongs to the field of seed treatment. According to the method for promoting seed germination, novel carbon nano quantum dots are used as an initiator to treat medicago sativa seeds; after the alfalfa seeds are subjected to initiation treatment, the drought resistance of the alfalfa seeds can be remarkably improved, seed germination and seedling growth can be promoted, the germination rate can be increased by 23%, the germination potential can be increased by 34%, the plant height can be increased by 90%, the biomass can be increased by 70%, meanwhile, the antioxidant enzyme activity can be remarkably improved by 33.1%, and the malondialdehyde content can be remarkably reduced by 26.43%. By applying the seed priming method, the planting efficiency can be improved, and meanwhile, the planting cost is reduced.

The invention discloses an application of an OPA1-enriched exosome in preparation of a medicine for improving mitochondrial dysfunction. The preparation method comprises the following steps: screening by adopting a phage display technology to obtain a CD9-targeted polypeptide P13, forming a fusion protein OPA1-P13 from the P13 and OPA1, constructing a recombinant plasmid for expressing the fusion protein, transfecting a cell for co-expression, and extracting the exosome, thereby obtaining the OPA1-enriched exosome OPA1-Exo. The OPA1-Exo is used for treating the mitochondrial dysfunction, and the result shows that the OPA1-Exo can activate antioxidant enzyme to reduce the ROS level in cells, promote an oxidative phosphorylation pathway to improve the ATP content of mitochondria, obviously improve a mitochondrial network and recover the mitochondrial dysfunction, so that the obvious mitochondrial function repair potential is shown. According to the invention, the OPA1 protein is successfully used for treating the mitochondrial dysfunction in the form of the exosome.

The invention relates to the technical field of preparation methods of nutritional products, in particular to a preparation method of ganoderma lucidum spore oil with a liver regulating effect. Comprising the steps of preparing ganoderma lucidum spore powder, extracting ganoderma lucidum spore oil and enriching the ganoderma lucidum spore oil. Ganoderma lucidum blocks are treated through an ultrasonic extraction technology, ganoderma lucidum spore powder is prepared through a spray drying method, n-hexyl alcohol is used for soaking extraction, high-purity ganoderma lucidum spore oil is obtained through vacuum concentration and molecular distillation, and residual solvent is removed through film evaporation. Active ingredients such as triterpenoids and unsaturated fatty acid are further enriched by using supercritical CO2 extraction; the extraction efficiency and the purity of active ingredients of the ganoderma lucidum spore oil are remarkably improved, the obtained ganoderma lucidum spore oil has remarkable effects in the aspects of reducing liver toxicity indexes (ALT and AST) and enhancing antioxidant enzyme activity (SOD and GSH-Px), the operation controllability is high, the ganoderma lucidum spore oil is suitable for large-scale industrial application, and technical support is provided for research and development of liver protection drugs and functional food.