53 results about "Antioxidative enzyme" patented technology

The application discloses application of lactobacillus rhamnosus in preparation of a medicine for treating osteoporosis, relates to the technical field of biological medicine, and the osteoporosis is osteoporosis caused by estrogen deficiency; the medicine is used for inhibiting osteoclast differentiation to play the role of improving osteoporosis; the lactobacillus rhamnosus in the medicine is used for inhibiting the generation of active oxygen to inhibit osteoclast differentiation, thereby improving the osteoporosis caused by estrogen deficiency; the lactobacillus rhamnosus in the medicine is used for activating a nuclear factor Nrf2 signal path and downstream antioxidant enzymes to play an antioxidation stress role; the lactobacillus rhamnosus in the medicine is used for activating an Nrf2-mediated antioxidation path, inhibiting active oxygen generation, and then hindering osteoclast differentiation and function, thereby playing the role of improving osteoporosis; the lactobacillus rhamnosus provides a brand-new medicine treatment strategy for osteoporosis.

The present application relates to the field of biological medicine and modernization of traditional Chinese medicine, and particularly relates to a therapeutic target, an inhibitor and a traditional Chinese medicine composition for treating ovarian hypofunction caused by oxidative stress and application thereof. The present application first determines SRC tyrosine kinase as a key drug target for preventing and treating the disease. The application of the SRC tyrosine kinase inhibitor (such as secaitinib) in preparing related drugs is provided, which can alleviate the damage of ovarian granulosa cells by inhibiting the SRC activity, down-regulating the expression of antioxidant enzyme related genes and reducing the level of active oxygen. Meanwhile, the present application provides a traditional Chinese medicine composition composed of mulberry, kudzu root, tuckahoe and medlar. The composition can improve the hormone level, enhance the antioxidant capacity, improve the ovarian function and fertility by inhibiting the SRC tyrosine kinase activity and the SRC-RAF1-MEK-ERK signal pathway. The present application provides a new solution for the targeted treatment of ovarian hypofunction and the modernization of traditional Chinese medicine.

The application provides an engineered probiotic with intracellular chimeric nanoscale enzyme, which is a probiotic modified with several antioxidant enzymes on an inner membrane; the antioxidant enzyme is composed of a metal oxide nanoscale enzyme with a particle size less than 10 nm and a targeting molecular structure and a saccharide molecular structure modified on the surface of the metal oxide nanoscale enzyme; the targeting molecular structure and the saccharide molecular structure are connected to the surface of the metal oxide nanoscale enzyme through a connecting group containing a metal ligand structure based on coordination and electrostatic interaction, and the antioxidant enzyme accounts for 5-20% of the mass of the engineered probiotic. The application also provides a method for preparing the engineered probiotic and application of the engineered probiotic in preparation of a medicine or food for preventing or treating antibiotic-associated diarrhea. The engineered probiotic can realize efficient elimination of hydroxyl radicals near a membrane induced by an antibiotic, so as to accurately inhibit a lipid peroxidation reaction and protect the integrity of a cell membrane.

This invention relates to the field of bioactive peptide preparation technology, and discloses a method for preparing and applying a bifunctional antioxidant and anti-inflammatory peptide based on porcine skin collagen. The bifunctional peptide has the amino acid sequence GPSGPPGEKGP. Through peptidomics and computer simulation screening, this invention identified five novel antioxidant peptides with good solubility, non-sensitizing properties, and non-toxicity. Using an AFB1-induced HuH7 cell oxidative damage model, in in vitro cell experiments, GP-11 was shown to significantly reduce ALT, AST activity, and ROS levels, enhance the activity of SOD, CAT, and GSH-Px antioxidant enzymes, and activate the Keap1-Nrf2 pathway. It also reduced the levels of inflammatory factors TNF-α and IL-6 by downregulating the classical cGAS-STING inflammatory pathway. GP-11 exhibits both significant antioxidant effects and the ability to reduce the levels of inflammatory factors, demonstrating a dual function of antioxidation and anti-inflammation.

This invention discloses a pharmaceutical composition for treating asthenospermia by activating the Nrf2 / HO-1 / GPx4 pathway and its application. The pharmaceutical composition comprises taurine and geranium as active ingredients, wherein the mass ratio of taurine to geranium is 3-5:1. The pharmaceutical composition of this invention can synergistically activate the Nrf2 / HO-1 / GPx4 antioxidant signaling pathway, upregulate the expression of downstream antioxidant enzymes GPx4 and HO-1, inhibit lipid peroxidation, protect sperm mitochondrial function, and simultaneously improve the microenvironmental metabolic profile of the epididymis—the site of sperm maturation—thereby enhancing sperm motility. The pharmaceutical composition provided by this invention can be used to prepare a treatment for asthenospermia, particularly oxidative stress-induced asthenospermia.

This invention discloses a method for extending the storage time of peaches, a composite plasma-activated water system, and a preservation system, belonging to the field of post-harvest preservation of fruits and vegetables. The method uses a quaternary gas mixture of argon, oxygen, carbon dioxide, and helium to prepare plasma-activated water through discharge. A specific ratio of tea polyphenols and chitosan is then added to obtain composite plasma-activated water. Combined with gentle pre-cooling, light-protected ultrasonic atomization spraying, and modified atmosphere buffering, this achieves a synergistic effect of antibacterial activity, water retention, antioxidant properties, and flavor preservation. This method can reduce the total bacterial count, rot rate, and weight loss rate of peaches during storage, maintain fruit firmness, enhance the activity of endogenous antioxidant enzymes, reduce malondialdehyde accumulation, and effectively retain soluble solids and titratable acids, among other flavor compounds. The process of this invention is green and mild, with no chemical residues, and can delay flesh softening and peel browning, thus extending the storage time and shelf life of peaches.

This invention discloses a method and application for improving wheat drought stress resistance based on the transcription factor TaHY5, relating to the fields of plant genetic engineering, wheat genetic breeding, and crop stress resistance improvement. This invention involves constructing a TaHY5 overexpression vector or a CRISPR / Cas9 editing vector, and transforming wheat immature embryos / callus tissue using Agrobacterium-mediated transformation or gene gun methods to obtain transgenic and gene-edited materials. After drought stress treatment, indicators such as biomass, malondialdehyde (MDA) content, and electrolyte permeability are measured to comprehensively evaluate drought resistance. Results show that TaHY5 overexpression significantly increases wheat biomass, APX, CAT and other antioxidant enzyme activities, as well as soluble protein and GSH content under drought stress, while reducing MDA accumulation and electrolyte permeability, and enhancing photosynthetic maintenance, reactive oxygen species scavenging, osmotic regulation, and cell membrane stability. This invention is the first to demonstrate that TaHY5 can synergistically improve wheat drought resistance, providing a simple and comprehensive improvement scheme, and offering new gene resources and practical technical pathways for wheat drought-resistant breeding.

This invention discloses a novel application of the RNA-binding protein HNRNPA1 in regulating copper death in synovial fibroblasts of rheumatoid arthritis (RA-FLS). Overexpression of HNRNPA1 recognizes and binds to the m6A modification site of SLC3A2 mRNA, enhancing its transcript stability, restoring the expression of SLC3A2 and copper death-related proteins, reducing MDA levels, increasing copper ion concentration and the activity of antioxidant enzymes SOD and POD, thereby repairing mitochondrial ultrastructural damage, inhibiting abnormal cell proliferation, and inducing apoptosis. Reversal experiments confirmed that knocking down SLC3A2 reverses the above effects, indicating that SLC3A2 is a core downstream target of HNRNPA1-mediated copper death. This invention reveals for the first time the decisive role of the HNRNPA1-SLC3A2-copper death axis in the pathological phenotype of RA-FLS, providing a new target for molecular intervention in rheumatoid arthritis.

This invention discloses a Polygonatum sibiricum vesicle preparation for treating premature ovarian failure, its preparation method, and its application. The preparation method includes: cleaning Polygonatum sibiricum, juicing, filtering to remove residue, and collecting the juice; subjecting the juice to gradient centrifugation to remove residual plant tissue, plant cells, and plant cell debris; then using an extraction reagent for stepwise precipitation, collecting the precipitate, and resuspending it to obtain the final product. The Polygonatum sibiricum vesicle preparation prepared by this invention can be efficiently taken up by ovarian granulosa cells. It effectively treats premature ovarian failure by activating the Nrf2 signaling pathway, upregulating antioxidant enzyme expression, scavenging reactive oxygen species, restoring mitochondrial membrane potential, inhibiting apoptosis, and restoring the expression levels of estrogen, follicle-stimulating hormone, and anti-Müllerian hormone. The preparation method of this invention is standardized, biocompatible, and safe, and has broad clinical application prospects.

The present application belongs to the technical field of compound feed for marine fish, and particularly relates to a feed for improving the antioxidant enzyme activity of juvenile Hexagrammos otakii and a preparation method thereof. The feed for improving the antioxidant enzyme activity of juvenile Hexagrammos otakii is prepared from the following raw materials in mass percentage: 95% to 96.5% of basic ingredients, 1% of compound vitamins, 1% of compound mineral salts, and 1.5% to 3% of an antioxidant enzyme activity composition, and the sum of the mass percentages of the raw materials is 100%; the antioxidant enzyme activity composition is composed of vitamin C and beta-carotene, and the mass ratio of vitamin C to beta-carotene is 1 to 3: 1 to 3. The feed for improving the antioxidant enzyme activity of juvenile Hexagrammos otakii obtained by the present application not only can provide sufficient natural antioxidant components to enhance the antioxidant defense mechanism of the juvenile fish, but also can effectively solve the problems of slow growth, low feed utilization rate, and water pollution of juvenile Hexagrammos otakii caused by insufficient antioxidant activity.

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

The present application relates to the application of active ingredients in Lonicera japonica in antioxidant stress, the active ingredients in Lonicera japonica break the Keap1-Nrf2 protein-protein interaction in cells, activate Nrf2 pathway, and target binding Keap1 protein, release Nrf2 protein, and activate the mode of antioxidant enzyme to play the role of antioxidant.The present application first proves by experiment that the active ingredients in Lonicera japonica target binding Keap1 protein and break Keap1-Nrf2 PPI, release Nrf2 protein, and activate the expression of downstream antioxidant enzymes (such as NQO1), so as to realize the effect of antioxidant.

The application discloses a kind of antifreeze for preventing and treating late frost injury of forsythia suspensa and its preparation method and application, belong to antifreeze technical field.The antifreeze for preventing and treating late frost injury of forsythia suspensa is prepared by using potassium phosphate, brassinolide, boron, amino acid and saccharide, and the cell freezing point is reduced by using potassium phosphate and saccharide for osmotic regulation, ice crystal formation is inhibited, while boron and amino acid jointly stabilize cell membrane and cell wall structure, and mechanical damage caused by low temperature is reduced;Brassinolide as key regulatory signal, can activate the antioxidant enzyme system in vivo, effectively remove active oxygen accumulated under low temperature stress, and relieve oxidative damage;Amino acid and saccharide provide direct nutrition and energy support for flowering period, promote the accumulation of protective substances, and maintain metabolic balance.Each component is synergized through multiple pathways of "osmotic protection-structure reinforcement-oxidative defense-metabolic support", so that the overall cold resistance is significantly improved.

The present application relates to the technical field of biological materials, and particularly relates to a preparation method of a two-stage microneedle for regulating reactive oxygen species homeostasis and application thereof.The present application combines two complementary materials of Fe-MOF for oxidative antibiosis and CeO2 for ROS removal to construct a two-stage microneedle for regulating reactive oxygen species, which can not only realize local delivery of drugs, but also effectively treat periodontitis through antibiosis, ROS balance and bone promotion.The present application utilizes Fe-MOF and CeO2 nanomaterials to synergistically regulate ROS level, Fe-MOF catalyzes Fenton-like reaction to generate ROS in the periodontal pocket microenvironment, realizes efficient, rapid and broad-spectrum antibiosis, and compared with traditional antibiotics, can reduce the risk of drug resistance, while CeO2 has excellent antioxidant enzyme simulation activity, removes excess ROS and reduces inflammatory response, and the innovative combination of the two materials solves the core contradiction between efficient antibiosis demand and oxidative stress damage in the treatment of periodontitis.

This invention discloses a highly efficient strain of *Bacillus tekirii* BB that degrades PLA microplastics and its applications. This strain was deposited on February 2, 2026, at the China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing, with accession number CGMCC NO. 37630. This strain can secrete multiple enzyme systems, including lipase, amylase, laccase, and catalase. In soil systems, this strain can effectively degrade PLA, increase soil lactic acid content and the activity of various soil enzymes, and improve soil organic matter, nitrogen, phosphorus, and potassium nutrient status. This strain can alleviate the growth stress of PLA pollution on plants, increasing the plant height, biomass, and antioxidant enzyme activity of crops such as buckwheat. This strain combines PLA degradation, soil remediation, and plant growth promotion functions, making it suitable for in-situ bioremediation of PLA microplastic pollution in farmland and possessing significant environmental and agricultural application value.

This invention discloses nanoparticles based on transcytosis-activating ligands, their preparation method, and applications, particularly in the treatment of fundus diseases. The nanoparticles comprise an enzyme protein core and a polymer shell grown in situ on the protein surface. The polymer shell contains positively charged monomers, transcytosis-activating ligands, and neutral monomers. The nanoparticles can enhance the hydrolytic resistance of enzyme proteins (e.g., antioxidant enzymes), protecting them from degradation during transport and improving delivery stability. Simultaneously, the nanoparticles can also promote the efficient delivery of proteases (especially to the posterior segment of the eye), exhibiting good safety and showing excellent application prospects in the medical field.

This invention discloses an S-estrol composition for delaying ovarian aging and its uses, relating to the fields of biomedicine and health products. The S-estrol composition for delaying ovarian aging comprises, by weight, the following components: 1-5 parts S-estrol, 2-8 parts soy isoflavones, 3-10 parts coenzyme Q10, 2-6 parts vitamin E, 4-12 parts vitamin C, 0.01-0.05 parts folic acid, 0.1-0.3 parts zinc source, 0.5-1.5 parts anti-aging protective agent, 50-80 parts carrier, and 10-15 parts purified water. This invention uses high-purity S-estrol as the core, combined with soy isoflavones, coenzyme Q10 and a variety of antioxidant nutrients in a scientific formulation to form a synergistic system for estrogen receptor signal regulation and antioxidant defense. By constructing a complex antioxidant network and introducing novel anti-aging protectants for synergistic effect, the activity of antioxidant enzymes tends to increase and the level of lipid peroxidation tends to decrease, thereby improving the oxidative stress state in ovarian cells as a whole.

The application belongs to the technical field of biological nanomaterials, and particularly relates to a nano-enzyme material and a preparation method and application thereof. The application reacts dimethyl imidazole and metal salt in an aqueous solution to obtain a metal-dimethyl imidazole composite material solution, wherein the metal salt comprises copper salt and / or zinc salt; and reacts the metal-dimethyl imidazole composite material solution and tannic acid to obtain a nano-enzyme material. The nano-enzyme material provided by the application has excellent antioxidant enzyme-like catalytic activity and good biocompatibility, can effectively remove abnormally increased reactive oxygen species (ROS) in the process of myocardial infarction and myocardial ischemia-reperfusion injury, inhibit inflammatory response and myocardial cell apoptosis, improve the mitochondrial function state, thereby promoting the repair of damaged myocardial tissue and significantly improving the cardiac function, and provides a new technical solution for the treatment of myocardial infarction and related ischemic cardiovascular diseases.

The present application relates to the technical field of plant genetic engineering, and specifically provides the role of protein kinase gene GmCIPK6 in regulating soybean salt tolerance. The GmCIPK6 gene is cloned from soybean, and the GmCIPK6 overexpression and CRISPR / Cas9 knockout transgenic soybean plants are obtained by Agrobacterium-mediated genetic transformation technology. The results show that under salt stress, the leaf damage degree of the overexpression plants is significantly lower than that of the wild type, while the damage degree of the knockout plants is significantly aggravated; at the same time, the activities of antioxidant enzymes CAT, POD and APX in the overexpression plants are significantly increased, while those in the knockout plants are significantly decreased. Transcriptome analysis shows that the differentially expressed genes are significantly enriched in the hydrogen peroxide catalytic pathway and the oxidative stress related pathway. The present application confirms that the GmCIPK6 gene positively regulates the salt tolerance of soybean by regulating the antioxidant enzyme system, and provides an effective gene resource for breeding new salt-tolerant soybean varieties.

This invention relates to the field of bioactive peptide technology, and discloses a tetrapeptide with cognitive-improving function and its applications. The primary amino acid sequence of the tetrapeptide is Lys-Gly-Phe-Pro, and its molecular weight is 489.2587 Da. The tetrapeptide of this invention can be chemically synthesized or directionally prepared from sea cucumber protease hydrolysates. The tetrapeptide of this invention exhibits cognitive-improving activity, mainly through inhibiting GABA. B The expression of R activates the cAMP / PKA / CREB signaling pathway, promotes the release of the inhibitory neurotransmitter GABA, reduces oxidative stress damage, increases the gene expression of neurotrophic factors Bdnf and Nt3, and significantly upregulates the gene expression of antioxidant enzymes Sod1 and Gpx1, thereby exerting a neuroprotective effect and improving age-induced cognitive impairment.

The present application relates to a kind of mRNA delivery system based on liposome with antioxidant efficacy, and functional cosmetic composition containing the system as active ingredient.The mRNA delivery system based on liposome and cosmetic composition of the present application can directly induce the production of antioxidant enzyme in skin cells, thereby providing sustained and effective antioxidant effect.This enables the mRNA delivery system to penetrate into the deep layer of skin, exerting antioxidant effect in epidermis and dermis, thereby enhancing the antioxidant system of skin cells themselves without relying on externally supplied antioxidants.In addition, it can also effectively prevent and improve the problems of skin aging, inflammation, pigmentation and the like caused by oxidative stress, and by applying mRNA technology to the field of cosmetics, it provides an innovative approach to lead a new paradigm for the cosmetic industry.

The present application relates to a kind of egg-laying hens premix with DHA efficient enrichment and egg preservation, and application thereof, belong to animal nutrition and feed technology field.The present application first clearly that phospholipid deficiency is the core reason of limiting DHA enrichment efficiency, reveals that soy lecithin promotes the molecular mechanism of DHA deposition by up-regulating FABP, PCTP expression, and proves that astaxanthin through direct free radical scavenging and the double synergistic antioxidant mechanism of activating endogenous antioxidant enzyme system.Based on this, with microencapsulated schizochytrium sp powder, high-purity soy lecithin, astaxanthin as core component, compound probiotics, lutein, organic selenium, construct four-effect synergistic premix formula, and establish "DHA enrichment-egg quality-shelf life" linkage parameter system.Application of the present application, egg DHA enrichment amount reaches 500~1000 mg / 100 g yolk, feeding 2 weeks stable enrichment, egg production rate keeps more than 85%, shelf life is extended by 15~20 days, with efficient nutrition fortification and preservation function.

This invention discloses the application of purified lignin and oxygen-alkali treated lignin in alleviating salt stress in plants. The purified lignin and oxygen-alkali treated lignin were prepared into aqueous solutions of appropriate concentrations and applied to plants under salt stress through hydroponics or soil cultivation systems. Results showed that exogenous application of both lignins alleviated the growth inhibition caused by salt stress to a certain extent, effectively improving the growth status of plants in saline environments. This was mainly manifested in the recovery of apparent biomass, improvement in leaf photosynthetic pigment content and photosynthetic rate, and increased antioxidant enzyme activity. This invention uses low-cost, high-activity, and environmentally friendly purified lignin as a novel plant growth regulator, providing an economical, green, and scalable new approach for the efficient cultivation of crops in saline soils.

This invention discloses a cultivation substrate for promoting the growth of Acanthopanax senticosus seedlings and its application, belonging to the field of plant cultivation technology. The cultivation substrate for promoting the growth of Acanthopanax senticosus seedlings provided by this invention uses perlite and peat as a framework to construct a loose, breathable, water-retaining, and fertilizer-retaining physical structure. By adding Bacillus proteolyticus bacterial solution, its growth-promoting, antagonistic, and organic matter-decomposing effects are utilized to improve the rhizosphere microecology. Combined with controlled-release fertilizer, precise nutrient supply is achieved. These three elements synergistically optimize the water, fertilizer, air, and heat environment and microbial community in the rhizosphere of Acanthopanax senticosus seedlings, promoting nutrient absorption and metabolism, effectively improving photosynthetic efficiency and antioxidant enzyme (SOD, POD) activity; and significantly promoting the accumulation of medicinal components. The yield of eleutheroside E in the roots can reach 13.93 times that of the control group, and the yield of syringin in the leaves can reach 2279.82 μg, providing an efficient solution for cultivating high-quality, robust seedlings.

This application discloses the application of the BnaCT3 gene and its SNP molecular markers in regulating the cold resistance of rapeseed. The nucleotide sequence of the BnaCT3 gene is shown in SEQ ID NO.1. Overexpression of the BnaCT3 gene in rapeseed significantly improves the survival rate of plants under low-temperature stress, reduces electrolyte leakage rate and malondialdehyde and hydrogen peroxide content, increases proline content and antioxidant enzyme activity, and enhances the cold resistance of rapeseed. Furthermore, this application identifies two SNP sites located in the promoter region of the BnaCT3 gene, whose specific base combinations constitute H1 and H2 allele combinations. Rapeseed materials carrying the H2 allele exhibit significantly better cold resistance than those carrying the H1 allele, and all currently promoted varieties lack the H2 allele. This application provides effective gene resources and molecular markers for the genetic improvement of rapeseed cold resistance, which can be used for overexpression transgenic breeding and marker-assisted selection breeding.

This application belongs to the field of seed treatment technology and provides a rare earth coating agent for establishing alfalfa in Soda saline-alkali land and its preparation method. The coating agent is a cerium nitrate solution containing a film-forming agent, emulsifier, dispersant, defoamer, and wetting agent. The coating agent of this application can significantly overcome the ion toxicity and high pH stress in Soda saline-alkali land after seed coating, improve the germination rate and emergence rate of alfalfa in Soda saline-alkali land, increase plant height and root length, enhance the activity of antioxidant enzymes in the plant, and improve the establishment efficiency of alfalfa in Soda saline-alkali land.

The application discloses Luzhou-flavor liquor dreg water-soluble glucose oligosaccharide and a preparation method and application thereof, and belongs to the technical field of medicines.The application extracts water-soluble glucose oligosaccharide from Luzhou-flavor liquor dreg as raw material.The main chain of the Luzhou-flavor liquor dreg water-soluble glucose oligosaccharide is composed of glucose, and the side chain comprises arabinose and glucose; the Luzhou-flavor liquor dreg water-soluble glucose oligosaccharide is composed of arabinose, galactose, glucose and xylose, and the molar ratio is 8.81:2.54:76.82:6.90.The water-soluble glucose oligosaccharide prepared by the application can obviously improve the symptoms of alcoholic liver, and has the effects of inhibiting liver damage and improving the activity of liver antioxidant enzymes.In addition, the Luzhou-flavor dreg in the application is a by-product of liquor fermentation, has high raw material yield and low cost, and the application can improve the resource utilization value of the dreg.

This invention belongs to the field of genetic engineering technology, and more specifically, relates to a drought-responsive gene in larch trees. LgLEA10 Proteins and their applications. This invention provides a larch drought response gene. LgLEA10 The nucleotide sequence of the protein is shown in SEQ ID NO.1, and the amino acid sequence of the protein it expresses is shown in SEQ ID NO.2. This invention is the first to demonstrate that the Dahurian larch... LgLEA10 The gene is a regulatory gene for the larch's response to drought. This invention demonstrates through experiments that... LgLEA10 Gene overexpression can enhance the drought resistance of larch by significantly increasing the activity of antioxidant enzymes and osmotic regulators. This discovery provides important genetic resources and theoretical basis for breeding new larch varieties with strong drought resistance.