261 results about "Antioxidative enzyme" patented technology

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention discloses a preparation method and application of yak blood peptide with anti-hypoxia and anti-fatigue functions. The method comprises the steps of raw material pretreatment, composite enzymolysis (adopting a mixture of enzyme digestion modified lumbrukinase and nattokinase), enzyme deactivation, separation and purification, ferrous chelation, Maillard reaction, concentration and drying and the like. The enzymolysis efficiency and the active peptide yield are remarkably improved through ultrasonic treatment and a composite enzymolysis technology, the stability and the anti-hypoxia capacity of peptide fragments are enhanced through ferrous chelation, and the antioxidant activity and the anti-fatigue activity are further improved through the Maillard reaction. Experiments show that the yak blood peptide prepared by the method can significantly improve the antioxidant enzyme activity, prolong the normal-pressure hypoxia survival time, prolong the swimming exhaustion time and significantly reduce the MDA content of brain tissue and myocardial tissue. The obtained product can be applied to anti-hypoxia and anti-fatigue medicines or health-care foods, and has the advantages of high process efficiency, remarkable activity, high safety and the like.

The invention belongs to the technical field of biology, and particularly relates to application of serine in relieving heat stress of pleurotus ostreatus and a method. Under the heat stress condition, exogenous serine supply promotes pleurotus ostreatus hypha to recover growth, and the activity of antioxidant enzyme, the yield of sporocarp and the yield of nutrient substances are improved; when the activity of the PHGDH enzyme is inhibited, the serine synthesis is reduced; the hypha growth can be promoted by overexpressing the phgdh, and the enzymatic activity of the PHGDH can be improved; when acetyltransferases HAT, NAT1, NAT2 and NAT3 act on a K394 site of the PHGDH, the enzyme activity of the PHGDH can be improved, endogenous serine metabolism is activated to respond to heat stress, it is indicated that PHGDH-mediated serine metabolism plays an important role in corresponding heat stress of the pleurotus ostreatus, and the invention discloses a mechanism for improving heat resistance of the pleurotus ostreatus through the PHGDH-mediated serine metabolism. And a foundation can be laid for breeding high-temperature-resistant pleurotus ostreatus dominant strains.

The invention belongs to the technical field of medicines, and particularly relates to a plant lactobacillus for relieving acute alcoholic liver injury as well as a metagen and application of the plant lactobacillus. The strain of the plant lactobacillus L5-13 is preserved in the China Center for Type Culture Collection on August 18, 2025, and the preservation number of the strain is CCTCC NO: M 20251835. The strain and the metagen thereof can significantly improve pathological symptoms of mice with acute alcoholic liver injury, including: reducing the levels of ALT, AST, TC, TG and LDL-C in serum of the mice with acute alcoholic liver injury, and increasing the level of HDL-C; the abnormal blood lipid level of the mouse caused by acute alcohol is regulated; the activity of antioxidant enzymes in the liver is improved, and the lipid peroxidation level is reduced; the ethanol dehydrogenase activity of the liver is improved, and the ethanol metabolism capability is enhanced. Therefore, the strain and the metagen thereof have wide application prospects in prevention or treatment of acute alcoholic liver injury.

The invention relates to an application of a FaPDHE1alpha gene or an encoding protein thereof in prevention and control of strawberry gray mold, and belongs to the field of plant genetic engineering and the field of prevention and control of postharvest diseases of fruits and vegetables. The invention discloses a key positive regulation and control effect of FaPDHE1alpha in regulation and control of strawberry gray mold resistance. By constructing a 35S:: FaPDHE1alpha overexpression vector and a FaPDHE1alpha RNAi interference vector, according to transient gene overexpression and RNA interference silencing tests, it is verified that high expression of the FaPDHE1alpha gene can obviously enhance the resistance of strawberry fruits to botrytis cinerea, and silencing of the gene causes reduction of the disease resistance of the fruits. Mechanism studies show that after the FaPDHE1alpha gene is activated, mitochondrial energy metabolism and controlled ROS signal generation are promoted, antioxidant enzyme activity is enhanced, and defense related gene expression is induced, so that the disease resistance of fruits is improved. By means of functional genomics, the key function of the FaPDHE1alpha gene in prevention and control of gray mold after strawberry harvest is illuminated for the first time, and the FaPDHE1alpha gene can be used for screening disease-resistant breeding materials or developing biological prevention and control preparations for postharvest diseases and has important application value.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a GhHAT18 gene in regulation and control of drought stress resistance of plants, the nucleotide sequence of the GhHAT18 gene is shown as SEQ ID NO.1. The invention discloses that the cotton histone acetylase gene GhHAT18 can be used for regulating and controlling the drought resistance of the plants for the first time, and experiments show that compared with a wild type plant, after the expression of the GhHAT18 gene is inhibited, the GhHAT18 gene can be used for regulating and controlling the drought stress resistance of the plants. The antioxidant enzyme activity and the drought resistance of plants can be obviously reduced; and the transgenic plant over-expressed with the GhHAT18 shows stronger and higher antioxidant enzyme activity and drought resistance under the drought stress. Transcriptome analysis shows that the GhHAT18 gene affects the expression of drought-resistant related genes through a secondary metabolite synthesis pathway and an MAPK signal transduction pathway, and the adaptive capacity of plants to drought stress is further regulated and controlled.

The invention discloses a preparation method and application of cubilose peptide. The amino acid sequence of the cubilose peptide is KPAPPKPEPK. The cubilose peptide prepared by the invention has strong ultraviolet damage resistance, and can improve the cell survival rate, improve the antioxidant enzyme level in serum, scavenge free radicals and reduce the inflammatory factor level in skin tissues. When the cubilose peptide is used for developing anti-ultraviolet damage products, the efficacy of cubilose can be deeply excavated, and the quality and economic value of cubilose products are improved.

The invention discloses a whitening antioxidant composition containing a citrus chachiensis hortorum extract. The whitening antioxidant composition is prepared from the citrus chachiensis hortorum extract, ergothioneine and PDRN. The weight ratio of the citrus chachiensis hortorum extract to the ergothioneine to the PDRN is (0.1 to 5): (0.001 to 0.1): (0.01 to 1). Through ternary compounding of the citrus chachiensis hortorum extract, the ergothioneine and the PDRN, the synergistic effect of'inhibiting melanogenesis, removing ROS (reactive oxygen species) and improving antioxidant enzyme activity 'is realized, the expression of melanin precursor protein Pmel17 is inhibited, the levels of NNT and GSH-Px1 are improved, the content of reactive oxygen species (ROS) is reduced, and the problem of single effect in the prior art is solved. The composition has excellent whitening and anti-oxidation effects, is high in stability, good in safety and wide in application range, and can be widely applied to functional cosmetics such as whitening essence, face cream, emulsion and sunscreen cream.

The invention discloses application of a blueberry VcETR-1 gene in regulation of high temperature stress resistance of plants, and relates to the technical field of plant genetic engineering. According to the application of the blueberry VcETR-1 gene disclosed by the invention in regulating and controlling the high temperature stress resistance of the plant, the nucleotide sequence of the blueberry VcETR-1 gene is as shown in SEQ ID NO. 1. The gene can improve the stress resistance of a plant at a high temperature of 45 DEG C through overexpression, and can regulate the response of the plant to 200 [mu] mol / L of exogenous salicylic acid. Experiments show that 10-20% of leaves of the transgenic plant are still good in state after high-temperature treatment, and physiological indexes such as antioxidant enzyme activity and the like are better. The invention further provides a related application method and a primer pair for cloning the gene, the blank of research on the heat-resistant function of the blueberry ethylene receptor gene is filled, and an important basis and technical support are provided for cultivating new varieties of high-temperature-resistant plants.

The present application relates to the field of microorganism, and particularly to a strain of Buttiauxella agrestis 33, a vegetable fresh-keeping agent and application thereof.The present application provides a strain of Buttiauxella agrestis 33, and the preservation number of the strain is CGMCC No.34251.After the strain is used to treat green flower, the yellowing of the green flower after harvesting can be significantly inhibited.Then, the expression of genes related to chlorophyll metabolic pathway and antioxidant enzyme in the green flower treated by the Buttiauxella agrestis 33 is detected by real-time fluorescent quantitative reverse transcription PCR, and the result shows that the Buttiauxella agrestis 33 can inhibit the yellowing of the green flower after harvesting and improve the antioxidant capacity of the green flower by activating the expression of genes related to chlorophyll synthesis and antioxidant enzyme.

The invention relates to the technical field of nano-materials, and discloses a heterogeneous diatomic nano-enzyme as well as a preparation method and application thereof. The invention provides a space confinement regulation and control strategy which is used for constructing a heterodiatomic catalytic center on a nitrogen-doped carbon skeleton and preparing nano-enzyme with structural stability and synergistic catalytic performance. According to the method, a confinement framework formed by self-assembly of first metal ions such as cobalt ions and second metal ions such as zinc ions and organic ligands is used as a template, third metal ions such as iron ions are introduced, and then carbonization heat treatment is performed in a protective gas atmosphere, so that bimetal sites with stable bridging configuration are formed in situ. The obtained nano-enzyme has the catalytic activity of various antioxidant enzymes, including catalase and remarkable superoxide dismutase activity, and has more excellent free radical scavenging capacity compared with a single-metal or non-bridging system.

The present application relates to the field of biological medicine and modernization of traditional Chinese medicine, and particularly relates to a therapeutic target, an inhibitor and a traditional Chinese medicine composition for treating ovarian hypofunction caused by oxidative stress and application thereof. The present application first determines SRC tyrosine kinase as a key drug target for preventing and treating the disease. The application of the SRC tyrosine kinase inhibitor (such as secaitinib) in preparing related drugs is provided, which can alleviate the damage of ovarian granulosa cells by inhibiting the SRC activity, down-regulating the expression of antioxidant enzyme related genes and reducing the level of active oxygen. Meanwhile, the present application provides a traditional Chinese medicine composition composed of mulberry, kudzu root, tuckahoe and medlar. The composition can improve the hormone level, enhance the antioxidant capacity, improve the ovarian function and fertility by inhibiting the SRC tyrosine kinase activity and the SRC-RAF1-MEK-ERK signal pathway. The present application provides a new solution for the targeted treatment of ovarian hypofunction and the modernization of traditional Chinese medicine.

The application provides an engineered probiotic with intracellular chimeric nanoscale enzyme, which is a probiotic modified with several antioxidant enzymes on an inner membrane; the antioxidant enzyme is composed of a metal oxide nanoscale enzyme with a particle size less than 10 nm and a targeting molecular structure and a saccharide molecular structure modified on the surface of the metal oxide nanoscale enzyme; the targeting molecular structure and the saccharide molecular structure are connected to the surface of the metal oxide nanoscale enzyme through a connecting group containing a metal ligand structure based on coordination and electrostatic interaction, and the antioxidant enzyme accounts for 5-20% of the mass of the engineered probiotic. The application also provides a method for preparing the engineered probiotic and application of the engineered probiotic in preparation of a medicine or food for preventing or treating antibiotic-associated diarrhea. The engineered probiotic can realize efficient elimination of hydroxyl radicals near a membrane induced by an antibiotic, so as to accurately inhibit a lipid peroxidation reaction and protect the integrity of a cell membrane.

The invention belongs to the technical field of gene engineering, and particularly relates to a larch drought response gene LgLEA10, a protein and application thereof. The nucleotide sequence of the larch drought response gene LgLEA10 provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the protein expressed by the larch drought response gene LgLEA10 is as shown in SEQ ID NO.2. It is proved for the first time that the LgLEA10 gene of dahurian larch is a drought response regulation gene of dahurian larch. Tests prove that overexpression of the LgLEA10 gene can improve the drought resistance of larch by remarkably improving the activity of antioxidant enzyme and osmotic adjusting substances. The discovery provides important gene resources and theoretical basis for cultivating a new variety of larch with strong drought resistance.

The invention relates to the technical field of plant genetic engineering and agricultural planting, in particular to application and method of an SlGRXC9 gene in improvement of tomato cadmium stress tolerance, the number of the SlGRXC9 gene is Solyc07g053550, the core coding region sequence of the SlGRXC9 gene is SEQ ID NO.1, and the coded protein sequence is SEQ ID NO.2; through construction of SlGRXC9 overexpression transgenic tomatoes, heterologous expression of protein encoded by the gene in plant cells or use of the gene as a molecular breeding target, the system activity of antioxidant enzymes such as SOD, POD, CAT and GR in tomatoes can be enhanced, cadmium stress induced H2O2 and O2-accumulation and membrane lipid peroxidation damage are reduced, and absorption and accumulation of the tomatoes to cadmium ions are not affected; according to the method, the growth ability and physiological stability of the tomatoes under cadmium stress are remarkably improved, the risk of nutrient imbalance is avoided, the hereditary stability is high, and the method can be widely applied to safe production of the tomatoes in cadmium-contaminated soil and creation of stress-tolerant germplasm.

The invention provides a highland barley HvDREB3 protein, a coding gene and application thereof, and belongs to the technical field of plant genetic engineering. The HvDREB3 protein provided by the invention has an amino acid sequence as shown in SEQ ID NO.1, and the coding gene of the HvDREB3 protein has a nucleotide sequence as shown in SEQ ID NO.2. The invention also provides application of the protein, the coding gene of the protein or a biological material containing the coding gene in regulation and control of plant freezing resistance, and a method for constructing an anti-freezing plant. The HvDREB3 gene is subjected to overexpression in highland barley through an overexpression transgenic technology, so that the survival rate is remarkably increased, the ion leakage rate is remarkably reduced, the chlorophyll content is increased, the antioxidant enzyme activity is enhanced, and the capability of resisting low-temperature freezing injury stress is remarkably improved.

This invention relates to the field of bioactive peptide preparation technology, and discloses a method for preparing and applying a bifunctional antioxidant and anti-inflammatory peptide based on porcine skin collagen. The bifunctional peptide has the amino acid sequence GPSGPPGEKGP. Through peptidomics and computer simulation screening, this invention identified five novel antioxidant peptides with good solubility, non-sensitizing properties, and non-toxicity. Using an AFB1-induced HuH7 cell oxidative damage model, in in vitro cell experiments, GP-11 was shown to significantly reduce ALT, AST activity, and ROS levels, enhance the activity of SOD, CAT, and GSH-Px antioxidant enzymes, and activate the Keap1-Nrf2 pathway. It also reduced the levels of inflammatory factors TNF-α and IL-6 by downregulating the classical cGAS-STING inflammatory pathway. GP-11 exhibits both significant antioxidant effects and the ability to reduce the levels of inflammatory factors, demonstrating a dual function of antioxidation and anti-inflammation.

This invention discloses a pharmaceutical composition for treating asthenospermia by activating the Nrf2 / HO-1 / GPx4 pathway and its application. The pharmaceutical composition comprises taurine and geranium as active ingredients, wherein the mass ratio of taurine to geranium is 3-5:1. The pharmaceutical composition of this invention can synergistically activate the Nrf2 / HO-1 / GPx4 antioxidant signaling pathway, upregulate the expression of downstream antioxidant enzymes GPx4 and HO-1, inhibit lipid peroxidation, protect sperm mitochondrial function, and simultaneously improve the microenvironmental metabolic profile of the epididymis—the site of sperm maturation—thereby enhancing sperm motility. The pharmaceutical composition provided by this invention can be used to prepare a treatment for asthenospermia, particularly oxidative stress-induced asthenospermia.

The invention discloses an ICAM-1 targeting engineered exosome-metal phenol nano-drug as well as a preparation method and application thereof. The nano-drug is composed of an engineered exosome and metal phenol nano-particles, wherein ICAM-1 targeting VHH antibodies are displayed on the surface of the engineered exosome, and the metal phenol nano-particles are encapsulated in the engineered exosome and formed by self-assembly of iron ions and resveratrol. The MPNs are efficiently loaded into the exosome through an electroporation method, and a composite nano delivery system (ICAM (at) Exo (at) MPNs) is constructed. The system has multiple advantages of active targeting of kidney inflammation parts, active oxygen responsive drug release, simulation of antioxidant enzyme activity, regulation and control of macrophage functions and the like. The ICAM (at) Exo (at) MPNs can remarkably relieve kidney crystal deposition, oxidative stress and inflammatory response induced by calcium oxalate, and the protective effect of the ICAM (at) Exo (at) MPNs is related to regulation and control of an SLC1A7 / MerTK signal channel. The invention provides an efficient and safe new strategy for preventing and treating the calcium oxalate kidney injury.

This invention discloses a cotton high-temperature yield-increasing regulator and its application. The regulator, water-based, comprises 2-amino-3-methylhexanoic acid and 1-methylcyclopropene. When applied during the cotton flowering and boll-forming stage, it significantly improves cotton photosynthetic performance and antioxidant enzyme activity, enhancing the cotton's high-temperature resistance. Compared to spraying with plain water, spraying this regulator at high temperatures increased boll weight by 95.2%, reduced shedding rate by 6.9%, and increased seed cotton yield by 74.1%, indicating that under high-temperature stress, this high-temperature yield-increasing regulator can significantly increase seed cotton yield by increasing boll weight and reducing shedding rate. In field trials, compared to a control using room-temperature water, spraying this high-temperature yield-increasing regulator also significantly reduced cotton boll shedding rate (average reduction of 11.1%) and significantly increased cotton seed cotton yield (average increase of 15.4%). This high-temperature yield-increasing regulator has advantages such as ease of use, small dosage, low cost, good effect, safety, high efficiency, and environmental friendliness, and has broad application prospects.

This invention discloses a method for extending the storage time of peaches, a composite plasma-activated water system, and a preservation system, belonging to the field of post-harvest preservation of fruits and vegetables. The method uses a quaternary gas mixture of argon, oxygen, carbon dioxide, and helium to prepare plasma-activated water through discharge. A specific ratio of tea polyphenols and chitosan is then added to obtain composite plasma-activated water. Combined with gentle pre-cooling, light-protected ultrasonic atomization spraying, and modified atmosphere buffering, this achieves a synergistic effect of antibacterial activity, water retention, antioxidant properties, and flavor preservation. This method can reduce the total bacterial count, rot rate, and weight loss rate of peaches during storage, maintain fruit firmness, enhance the activity of endogenous antioxidant enzymes, reduce malondialdehyde accumulation, and effectively retain soluble solids and titratable acids, among other flavor compounds. The process of this invention is green and mild, with no chemical residues, and can delay flesh softening and peel browning, thus extending the storage time and shelf life of peaches.

The application provides application of a GmERF5 gene in drought resistance and salt stress resistance, and belongs to the technical field of genetic engineering.The GmERF5 gene is cloned into a pCAMBIA3301 vector to obtain a recombinant vector; the obtained recombinant vector is transformed into Agrobacterium rhizogenes, and a transgenic complex plant is obtained by using the obtained Agrobacterium rhizogenes to infect seeds, so that overexpression of the GmERF5 gene is realized.The overexpression of the GmERF5 gene can improve the antioxidant enzyme activity of the transgenic soybean plant, and the drought and salt stress resistance of the soybean is enhanced.The soybean GmERF5 of the application provides a new regulation gene resource for improving the drought resistance and salt tolerance of soybean, can be used for cultivation and improvement of stress-resistant soybean materials, lays a theoretical foundation for drought resistance and salt molecular mechanism, and simultaneously provides a theoretical basis and gene resource for soybean drought resistance and salt molecular breeding.

The invention provides fermented black beans with an anti-obesity effect as well as a preparation method and application of the fermented black beans, and relates to the field of fermented products. The preparation method comprises the following steps: soaking cleaned black beans with a polygonatum sibiricum enzymatic hydrolysate; sterilizing the soaked black beans, inoculating rhizopus, lactic acid bacillus and lactobacillus plantarum, and carrying out mixed fermentation; and drying the fermented black beans to obtain the fermented black beans with the anti-obesity effect. Through verification, the prepared fermented black beans can effectively relieve damage of a high-fat environment to the reproductive capacity of the nematodes, improve the automatic swimming capacity of the nematodes, improve the activity of antioxidant enzymes in the nematodes and remarkably reduce the content of triglyceride and free fatty acid in the high-fat nematodes, and the fermented black beans are proved to have a remarkable anti-obesity effect; meanwhile, the fermented black beans also have excellent sensory quality. The method overcomes the defects of poor sensory quality and poor function guidance of the fermented black beans in the prior art.

The invention discloses wheat malt seedling functional dietary fibers as well as a preparation method and application of the wheat malt seedling functional dietary fibers. The soluble dietary fiber and the insoluble dietary fiber of the wheat malt seedlings are extracted by adopting a wall breaking-extraction mode of synergy of biological enzyme and free radicals. The wheat malt seedling soluble dietary fibers can prolong the life of caenorhabditis elegans in an oxidative stress state, improve the antioxidant enzyme activity and improve the anti-apoptosis capability; the intestinal flora of the obese C57BL / 6J mouse induced by the high-fat feed is regulated; the wheat malt seedling insoluble dietary fiber has better binding water capacity, water-holding capacity and cholesterol and sodium cholate adsorption capacity, and can be used as a functional food additive for improving the texture and nutritional characteristics of food; and the wheat malt seedlings can also be used for assisting in reducing serum cholesterol level, preventing arteriosclerosis, promoting defecation, preventing constipation, colorectal cancer and the like in the field of health medical treatment, so that high-quality reutilization of the wheat malt seedlings is realized.

The invention discloses a cultivation method for promoting growth of watermelon grafted seedlings through composite multispectral light supplementation. The method comprises the steps that firstly, after light supplementation treatment is conducted on stocks, sampling is conducted, and the antioxidant capacity of the stocks is analyzed; secondly, after grafting culture is conducted on the rootstock after light supplementing treatment, optimal rootstock light supplementing treatment is determined, and meanwhile the grafting survival rate of the rootstock after grafting is measured; thirdly, performing grouped light supplementing treatment on the watermelon grafted survival seedlings, performing destructive sampling every 7 days after light supplementing is started, and recording sampling results; and finally, obtaining growth characteristic indexes of the watermelon grafted seedlings according to a sampling result, so that the optimal light supplementing intensity of the watermelon grafted seedlings is obtained through the growth characteristic indexes. According to the optimal light supplementing intensity, the growth of scions and stocks is remarkably promoted, the disease index is reduced, the growth of tendrils is inhibited, and the net photosynthetic rate, the total chlorophyll amount and the activity of antioxidant enzymes such as SOD and CAT are remarkably improved.

The application belongs to the technical field of feed additives, discloses a compound functional additive for relieving liver oxidative stress and endoplasmic reticulum stress of mandarin fish, and specifically discloses a feed additive which comprises the following components: sodium octanoate, sodium butyrate and rice husk powder. The additive provided by the application can effectively relieve liver oxidative stress and endoplasmic reticulum stress of mandarin fish, reduce the content of malondialdehyde, improve the ability of mandarin fish to resist oxidative enzyme activity, and is beneficial to the health of fish body.

This invention discloses a method and application for improving wheat drought stress resistance based on the transcription factor TaHY5, relating to the fields of plant genetic engineering, wheat genetic breeding, and crop stress resistance improvement. This invention involves constructing a TaHY5 overexpression vector or a CRISPR / Cas9 editing vector, and transforming wheat immature embryos / callus tissue using Agrobacterium-mediated transformation or gene gun methods to obtain transgenic and gene-edited materials. After drought stress treatment, indicators such as biomass, malondialdehyde (MDA) content, and electrolyte permeability are measured to comprehensively evaluate drought resistance. Results show that TaHY5 overexpression significantly increases wheat biomass, APX, CAT and other antioxidant enzyme activities, as well as soluble protein and GSH content under drought stress, while reducing MDA accumulation and electrolyte permeability, and enhancing photosynthetic maintenance, reactive oxygen species scavenging, osmotic regulation, and cell membrane stability. This invention is the first to demonstrate that TaHY5 can synergistically improve wheat drought resistance, providing a simple and comprehensive improvement scheme, and offering new gene resources and practical technical pathways for wheat drought-resistant breeding.

This invention discloses a novel application of the RNA-binding protein HNRNPA1 in regulating copper death in synovial fibroblasts of rheumatoid arthritis (RA-FLS). Overexpression of HNRNPA1 recognizes and binds to the m6A modification site of SLC3A2 mRNA, enhancing its transcript stability, restoring the expression of SLC3A2 and copper death-related proteins, reducing MDA levels, increasing copper ion concentration and the activity of antioxidant enzymes SOD and POD, thereby repairing mitochondrial ultrastructural damage, inhibiting abnormal cell proliferation, and inducing apoptosis. Reversal experiments confirmed that knocking down SLC3A2 reverses the above effects, indicating that SLC3A2 is a core downstream target of HNRNPA1-mediated copper death. This invention reveals for the first time the decisive role of the HNRNPA1-SLC3A2-copper death axis in the pathological phenotype of RA-FLS, providing a new target for molecular intervention in rheumatoid arthritis.