82 results about "Wild tomato" patented technology

The invention discloses a formula of a sour soup hotpot condiment and a making method of the sour soup hotpot condiment. According to the formula, the sour soup hotpot condiment comprises, by weight, 10 parts of wild tomatoes grown in Kaili, Guizhou Province, 0.5 part of salt, 0.5 part of spirit, 3-5 parts of fresh ginger, 3-5 parts of bamboo shoots, and 1-2 parts fresh red pepper. The making method of the sour soup hotpot condiment comprises the steps that all kinds of treated raw materials and auxiliaries are evenly mixed in a large porcelain basin which is sterilized through high-purity spirit; the evenly mixed raw materials and auxiliaries are placed in a pickle jar to be sealed and fermented, and the jar edge is filled with water; fermentation is conducted for 15 days at the temperature ranging from 22 DEG C and 28 DEG C, and then the finished sour soup hotpot condiment is formed. According to the formula of the sour soup hotpot condiment and the making method of the sour soup hotpot condiment, the used materials are all wild plants grown in the sour soup origin area, the sour of the sour soup is more resistant to boiling and pure, piquant taste is smooth and makes other food more delicious, the fishy smell of fish and meat which are added to a hotpot can be removed, and the sour soup hotpot condiment has the functions of clearing damp, promoting appetite and nourishing the stomach.

The invention discloses a method for cultivating grafting seedlings by utilizing tomato axillary buds. Lateral branches of aged tomato plants are processed through cold storage to avoid unsuited cultivation seasons for grafting of tomatoes, buds obtained through the grafting are used as scions, wild tomatoes are used as stocks, and grafting is carried out on the stocks and the scions to cultivate the grafting seedlings. According to the method for cultivating the grafting seedlings by utilizing the tomato axillary buds, it is guaranteed that the grafting seedlings of the tomatoes can be cultivated in appropriate seasons and crop rotation occasions, the grafting seedlings are high in evenness, easy to management after field planting and consistent in fruit maturation stage, the yield is equivalent to that of general seed bud grafting seedlings, seeds can be saved, and production cost is lowered.

The invention relates to a cultivation method for overcoming continuous cropping obstacle of eggplants, which is characterized by taking tomatoes or wilds tomatoes as parental stocks and taking coins as an eggplant variety; respectively culturing parental stock seedlings and coin seedlings, grafting and seedling by a cleft graft method to form eggplant graftings combining the parental stocks and the coins, fixedly planting the seedlings after the seedlings survive; making high plots or high ridges of 18cm, and fixedly planting the grafted seedlings in accordance with the row spacing of 70cm and the planting distance of 50cm, wherein the position of a grafting cutter point is 5cm higher than that of the ridge surface or the plot surface; carrying out plant adjustment by a double-rod pruning method after fixed planting to promote the germination of lateral branches, repeatedly carrying out topping treatment to generate multiple lateral branches, thus forming multiple fruits; and culturing until the eggplants mature. In the invention, the grafting and the root changing belong to the biological agronomic measures, the operation is simple, the application is convenient, no pollution is generated to environment and agricultural products during the process of production, and the high quality of the agricultural products can be maintained. The seed source of parental stocks is easily to obtain, the production cost is low, the effect on overcoming continuous cropping obstacle is obvious, and the purposes of continuously planting eggplants in the same block and plastic tent and realizing stable production can be achieved.

The invention relates to the field of genetic transformation, specifically to a wild tomato Solanum sitiens somatic embryo induction method and a somatic embryo genetic transformation method. The induction method provided by the invention comprises the following steps: (1) culturing Solanum sitiens aseptic seedlings;(2) carrying out dark culture and inducing roots, stems (without axillalry buds) and leaves of Solanum sitiens on a embryogenic callus induction medium ECIM to generate embryogenic callus;(3) transferring the generated embryogenic callus onto a somatic embryogenesis induction medium SEIM in the light to induce somatic embryogenesis; and (4) inducing budding and seedling.

The invention provides a sturgeon hotpot bottom material. The sturgeon hotpot bottom material is characterized by comprising a sour soup bottom material and a sturgeon cartilage material, wherein the sour soup bottom material comprises the following components in parts by weight: 20-30 parts of wild tomatoes, 5-10 parts of sweet wine, 3-5 parts of ginger, 3-5 parts of bamboo shoot, 1-2 parts of fresh chilli, 0.5 part of salt and 0.5 parts of wine. The sturgeon hotpot bottom material is rich in phytoene; after a fermenting process, the sturgeon hotpot bottom material can be more easily absorbed by eaters; the phytoene has the main functions of replenishing Qi and blood, strengthening the spleen and benefiting the stomach, and strengthening the heart and refreshing the mind; by a preparation method of the sturgeon cartilage material used in the sturgeon hotpot bottom material, various nutrients of sturgeon cartilage are retained to the maximum extent, so that the finally-prepared sturgeon cartilage material not only achieves a delicious flavoring effect and, but also has more nutritional properties.

The invention discloses an application of Lox (lipoxygenase) D gene of a tomato in regulating and controlling plant resistance. The nucleotide sequence of a DNA (deoxyribonucleic acid) segment provided by the invention is 106th-570th nucleotides from the tail end of 5' of sequence 2 in a sequence table. The invention also provides an application of LoxD protein and a coding gene thereof in regulating insect resistance and/or disease resistance of plants, wherein the amino acid sequence of LoxD protein is the sequence 1 in the sequence table, the nucleotide sequence of the LoxD protein coding gene is the sequence 2 in the sequence table. Experiments of the invention prove that a transgenic tomato can be obtained by inhibiting expression of a LoxD gene of a wild tomato, the disease resistance and insect resistance of the transgenic tomato are worse than those of the wild tomato, so that inhibition of expression of LoxD genes of plants lowers the insect resistance and disease resistance of the plants, and the LoxD gene is related to disease resistance of plants.

The invention relates to parthenocarpic tomatoes (Lycopersicon esculentum Mill.) which are grown from seeds, which are smaller than wild tomatoes and which, when ripe, display a more intense red colour than wild tomatoes. The tomatoes are obtained by cultivating a transgenic tomato plant which produces said parthenocarpic tomatoes and which is obtained using a method consisting in: (a) introducing a DNA construction into a cell or tissue of a tomato plant, said construction comprising (i) the pea END1 gene promoter or a functional fragment of same and (ii) a cytotoxic gene which is functionally bound to the aforementioned promoter or fragment of same; and (b) regenerating the tomato plant cell or tissue transformed in step (a) in order to produce a transgenic tomato plant that produces parthenocarpic tomatoes. The invention is suitable for use in food and agrifood industries.

The invention relates to the field of genetic transformation, and specifically relates to a method of establishing a regeneration system and a genetic transformation system of wild tomato solanum sities. The method comprises following steps: (1) solanum sitie aseptic seedlings are bred; (2) three different explants, namely the root, the stem, and leaves of solanum sities, are induced to produce callus tissue and multiple shoot points in a callus-inducing medium (CIM); (3) the induced multiple shoots are individually separated, then put in a aseptic seedling medium, and the multiple shoots are induced to generate roots and turn into seedlings in the aseptic seedling medium. Wild tomato solanum sities instead of modern technology clutivated tomatoes which have strong genotype specificity are chosen as the research objects, which benefit researches in tomato genetic engineering and the process in tomato molecular breeding. The built corresponding systems of solanum sities are suitable for the three explants, and the transformation medium and the culture conditions for the three explants are completely the same, therefore related research cost is largely reduced. More important, the method has advantages in that: the regeneration and transformation efficiencies are high, the system is stable and the testing time is short.

The invention discloses a method for improving insect resistance of tomato and provides application of protein or related biomaterials thereof in regulating plant insect resistance. The related biomaterials are nucleic acid molecules capable of expressing protein or an expression box, a recombinant vector, recombinant bacteria or a transgenic cell line containing the nucleic acid molecules, wherein the protein is SlMTB1 protein, SlMTB2 protein and SlMTB3 protein. Experiments prove that transgenic tomato with SlMTB1, SlMTB2 and SlMTB3 genes being silent can express more PI-II than wild tomato when being infringed by cotton bollworm, and resistance of the transgenic tomato to cotton bollworm is improved remarkably. Therefore, SlMTB1, SlMTB2 and SlMTB3 protein and part of coding genes thereofcan be used for cultivating and identifying insect-resistant plant varieties needed for agriculture and animal husbandry and ecological environment management and are of important significance in thefield of plant insect-resistant breeding.

The invention relates to a cultivated tomato SCF complex subunit CUL1 protein sequence and a nucleotide sequence thereof and belongs to the technical field of molecular biology. Concretely, the invention relates to a cultivated tomato SCF complex subunit CUL1 protein sequence shown as SEQ ID NO.3 and the nucleotide sequence which codes the CUL1 protein sequence. The CUL1 protein gene is utilized to screen out correlated or interactive substances, receptors, inhibitors, and antagonists. The CUL1 protein gene can be over-expressed or low-expressed through genetic engineering means, and furthermore the self-incompatibility mechanism mediated by S-nuclease is researched, so as to provide important theoretical foundation for obtaining wild tomato varieties which can overcome the incompatibility of distant hybridization and improving the tomato varieties.

The invention belongs to the technical field of genetic engineering and discloses a gene for increasing fruit setting rate and application thereof. The gene is Tkn4, the sequence of the gene is as shown in SEQ ID No. 1, the nucleotide sequence length of the gene is 1080 nucleotides, and the amino acid length of the gene is 335 amino acid residues. Compared with the fruit setting rate of wild tomato fruits, the fruit setting rate of the transgenic tomato fruits processed by the gene is increased evidently. Results show that the gene and a eukaryotic recombinant vector can evidently increase thefruit setting rate. The fruit setting rate is increased by a transgenic method. The gene obtained through genetic engineering cloning has important theoretical significance and actual application value in the regulating of a fruit setting process and the increasing of fruit yield.

The invention relates to a peach auxin primary response factor gene PpIAA1 and application thereof. The PpIAA1 gene is cloned from peach fruits, and the PpIAA1 gene is further stably transformed intoMicro-Tom tomatoes for functional verification. The results show that compared with wild tomatoes, root branches of PpIAA1 transgenic strain plants are decreased, the leaf color is obviously green, fruits are ripened in advance and the shelf life is shortened. And the mature period of the tomato fruits of the PpIAA1 transgenic strain is advanced by 6-8 days. The expression of the PpIAA1 is significantly inhibited by 1-MCP treatment of 'middle nectarine No. 13'. The expression of the PpIAA1 is significantly promoted by NAA or ethylene treatment of 'middle nectarine No. 16', indicating that thegene plays an important role in the ripening and softening process of peach fruits. The screened PpIAA1 gene can be helpful for researching the response mechanism that auxin and ethylene together promote the ripening of peach fruits in the ripening period of the peach fruits.

The invention discloses a fomes officinalis lung warming and phlegm resolving sour soup base and a preparation method thereof. The soup base is prepared from the following raw materials in parts by weight: 45 to 55 parts of fomes officinalis, 20 to 30 parts of Indian strawberry, 20 to 30 parts of changium smyrnioides, 15 to 20 parts of Japanese ardisia herb, 15 to 20 parts of curcuma tuber, 15 to 20 parts of licorice, 15 to 20 parts of ischnochiton hakodadensis, 10 to 15 parts of coelogyne cristata, 10 to 15 parts of mulberry bark, 10 to 15 parts of rheum officinale, 10 to 15 parts of Indian nightshade root, 10 to 15 parts of maianthemum bifolium, 25 to 35 parts of wild tomato, 15 to 20 parts of spine date seed, 15 to 20 parts of raspberry, 15 to 20 parts of shine skin papaya, 15 to 20 parts of cornus officinalis, 10 to 15 parts of toosendan fruit, and 10 to 15 parts of rice wine. The preparation method comprises the following steps: preparing raw materials, extracting raw materials, carrying out centrifugation, mixing, preparing tomato paste, mixing, grinding, mixing all raw materials, concentrating, and drying. The provided soup base has a sour and fresh taste, is palatable, is rich in nutrients, and has the functions of warming the lung, resolving phlegm, calming cough and panting, dispelling wind, and removing dampness, if people eat the soup base for a long term.

The invention discloses DNA molecule STTM-miR482a and application thereof. The DNA molecule has a nucleotide sequence shown as SEQ ID NO. 1. The invention further provides application of the DNA molecule, and a recombinant vector expressing the DNA molecule STTM-miR482a is used for regulating the expression of microRNA482a in plants. The specific method comprises the following steps: introducing the recombinant vector expressing the DNA molecule STTM-miR482a into a target plant, thereby obtaining the plant capable of regulating the expression of microRNA482a. Transgenic tomatoes are obtained by inhibiting the expression of miR482a in wild tomatoes, and compared with the wild tomatoes, the transgenic tomatoes have the advantage that the disease resistance is enhanced; and moreover, the DNA molecule STTM-miR482a disclosed by the invention has important significances for cultivating disease-resistant plant varieties so as to improve the crop yield.

A coral sour soup base removing nebula for improving eyesight and a preparing method thereof are disclosed. The soup base is prepared from 45-55 parts by weight of coral, 20-30 parts by weight of poria, 20-30 parts by weight of danshen root, 15-20 parts by weight of peony root, 15-20 parts by weight of pearl, 15-20 parts by weight of lily bulbs, 15-20 parts by weight of pleioblastus amarus leaves, 10-15 parts by weight of cordyceps sobolifera, 10-15 parts by weight of white mulberry root-bark, 10-15 parts by weight of borneolum blumeae, 10-15 parts by weight of flower of oriental paperbush, 10-15 parts by weight of spinach fruit, 25-35 parts by weight of wild tomatoes, 15-20 parts by weight of spine date seeds, 15-20 parts by weight of palmleaf raspberry fruit, 15-20 parts by weight of shine skin papaya, 15-20 parts by weight of asiatic cornelian cherry fruit, 10-15 parts by weight of szechwan chinaberry fruit and 10-15 parts by weight of rice wine. The preparing method comprises steps of preparing raw materials, extracting, centrifuging, mixing, preparing tomato paste, mixing, crushing, mixing all materials, concentrating and drying. The soup base is sour, refreshing and delicious in taste and rich in nutrition. Long-term eating of the soup base has functions of removing nebula for improving eyesight, calming mind, tranquilizing, removing dampness and promoting diuresis.

The invention relates to a combined vector construction method capable of increasing the content of coenzyme Q10 in tomatoes. The combined vector construction method comprises the following steps: obtaining an E8 promoter sequence, a tobacco Coq2 gene coding sequence, a DPS gene coding sequence, an HMGR2 gene coding sequence, a UbiC gene coding sequence and an hsp terminator sequence; constructingrespective level 0 vectors of the six sequences; constructing respective level 1 vectors of the four genes; and constructing a level 2 vector. The invention also relates to a production method of tomatoes capable of increasing the content of coenzyme Q10, which comprises the following steps: transforming agrobacterium GV3101 by using the combined vector obtained by the construction method throughthermal shock, and transforming wild tomatoes; and after obtaining independent resistant calluses, further differentiating and regenerating to obtain the tomato strain with high coenzyme Q10 content.Through cultivation and screening, the highest yield of T3-generation fruit coenzyme Q10 of the tomato strain HUCD can reach more than 7 times (-0.151 mg/g) of wild tomato fruits, and the tomato strain HUCD can be stably inherited and has no difference in phenotype; namely, the content of the coenzyme Q10 in the tomato strain HUCD is remarkably increased.

The invention relates to the technical field of agricultural production, in particular to a tomato grafting cultivation method, comprising the following steps: (1) selecting wild tomato with high disease resistance from rootstock; (2) 50 55 DEG C warm wat immersion 20 30 min, followed by 28 30 DEG C wat is soaked again for a period of time, and then place on a clean towel to squeeze out moisture; (3) accelerating sprouting and sowing when the sprouting rate reaches 85%; (4) 3 5 day of sowing, irrigate foot water, sprinkling medicinal soil on that bed surface, and sprinkling seed on the seedbed; (5) The seedbed temperature was maintained at 20 DEG C during the daytime 30 DEG C, night 15 18 DEG C, the temperature remained constant in daytime and 15 DEG C in nighttime after emergence; (6) 3 5 true leaves, with a knife blade in the distance between two true leaves 1 cm between a bevel knife, incision 0.6 0.7 cm, scion; (7) During the first three days after grafting, the daytime temperature remained at 25 27 DEG C, nighttime temperature 18 20 DEG C, constant humidity, shade; The tomato grafting method provided by the invention can effectively improve the survival rate and disease resistance of tomato grafting.

The invention provides a gene SLF participating in controlling intra-specific and inter-species reproductive disorder of solanaceae plants and application of the gene SLF, and discloses the gene SLF participating in controlling intra-specific and inter-species reproductive disorder of the solanaceae plants and egg white of codes of the gene SLF. A nucleotide sequence of the gene SLF is shown in the SEQ ID No:1-5, and an amino acid sequence of the egg white of the codes is shown in the SEQ ID No:6-10. The invention discloses a method that the gene SLF is implanted in petunia, tomato plants and other solanaceae plants through the genetic engineering technique, and therefore the intra-specific and inter-species reproductive disorder is eliminated. The invention further discloses the egg white utilizing polynucleotide and the codes thereof, and genetic breeding is conducted between planted tomato plants and wild tomato plants.

The invention discloses a plantain heat clearing and urination promoting sour soup base and a preparation method thereof. The soup base is prepared from the following raw materials in parts by weight: 45 to 55 parts of plantain, 20 to 30 parts of radix astragali, 20 to 30 parts of caulis sinomenii, 15 to 20 parts of cyathula root, 15 to 20 parts of curcuma tuber, 15 to 20 parts of corn silk, 15 to 20 parts of hypericum japonicum, 10 to 15 parts of white gourd peel, 10 to 15 parts of mulberry bark, 10 to 15 parts of rheum officinale, 10 to 15 parts of desmodium, 10 to 15 parts of lygodium japonicum, 25 to 35 parts of wild tomato, 15 to 20 parts of spine date seed, 15 to 20 parts of raspberry, 15 to 20 parts of shine skin papaya, 15 to 20 parts of cornus officinalis, 10 to 15 parts of toosendan fruit, and 10 to 15 parts of rice wine. The preparation method comprises the following steps: preparing raw materials, extracting raw materials, carrying out centrifugation, mixing, preparing tomato paste, mixing, grinding, mixing all raw materials, concentrating, and drying. The provided soup base has a sour and fresh taste, is palatable, is rich in nutrients, and has the functions of clearing heat, promoting urination, resolving phlegm, and cooling blood, if people eat the soup base for a long term.

The invention belongs to the technical field of biological grafting, and relates to a rootstock improvement method and application. The method includes step1, synthesizing a transfer gene; step 2, constructing the synthetic transfer gene and a functional gene on a vector; step 3, performing heterologous grafting with the tobacco transgenic in an agrobacterium infection mode as a stock and the wildtomatoes as scions; step 4, taking grafting openings and stem segments and flowers at different positions, extracting total RNA of plants, performing reverse transcription to obtain cDNA, and performing RT-PCR detection on transportation conditions of SlZFP2-tRNAMet, SlZFP2-3 * CTCT and the like in a heterologous grafting system; and step 5, investigating branching and flowering conditions of wild tomatoes on the transgenic rootstock. The method can be applied to fruit tree transgenic rootstock breeding, scion traits are efficiently improved in a fixed-point mode through rootstock transgenosis, rootstock genes are manually controlled, the scion traits are influenced, the regulation and control efficiency is improved, the breeding age limit is shortened, the manpower and material resourcecost is reduced, and the method is widely applied to various plants.

A tall gastrodia tuber sour soup base calming the liver to stop the wind and a preparing method thereof are disclosed. The soup base is prepared from 45-55 parts by weight of tall gastrodia tuber, 20-30 parts by weight of milkvetch root, 20-30 parts by weight of dogbane leaves, 15-20 parts by weight of peony root, 15-20 parts by weight of cuscuta chinensis lam., 15-20 parts by weight of liquorice root, 15-20 parts by weight of malaytea scurfpea fruit, 10-15 parts by weight of common yam rhizome, 10-15 parts by weight of white mulberry root-bark, 10-15 parts by weight of ginseng, 10-15 parts by weight of sanqi flower, 10-15 parts by weight of cordyceps sobolifera, 25-35 parts by weight of wild tomatoes, 15-20 parts by weight of spine date seeds, 15-20 parts by weight of palmleaf raspberry fruit, 15-20 parts by weight of shine skin papaya, 15-20 parts by weight of asiatic cornelian cherry fruit, 10-15 parts by weight of szechwan chinaberry fruit and 10-15 parts by weight of rice wine. The preparing method comprises steps of preparing raw materials, extracting, centrifuging, mixing, preparing tomato paste, mixing, crushing, mixing all materials, concentrating and drying. The soup base is sour, refreshing and delicious in taste and rich in nutrition. Long-term eating of the soup base has functions of calming the liver to stop the wind, supporting the healthy energy, enhancing body resistance, calming the liver, and calming nerves.

The invention discloses a fritillaria cirrhosa heat clearing and lung moistening sour soup base and a preparation method thereof. The soup base is prepared from the following raw materials in parts by weight: 45 to 55 parts of fritillaria cirrhosa, 20 to 30 parts of polygala root, 20 to 30 parts of pearl shell, 15 to 20 parts of red peony root, 15 to 20 parts of stemona, 15 to 20 parts of licorice, 15 to 20 parts of Chinese honey locust, 10 to 15 parts of cynanchum root and rhizome, 10 to 15 parts of mulberry bark, 10 to 15 parts of rheum officinale, 10 to 15 parts of boat-fruited sterculia seed, 10 to 15 parts of bitter apricot kernel, 25 to 35 parts of wild tomato, 15 to 20 parts of spine date seed, 15 to 20 parts of raspberry, 15 to 20 parts of shine skin papaya, 15 to 20 parts of cornus officinalis, 10 to 15 parts of toosendan fruit, and 10 to 15 parts of rice wine. The preparation method comprises the following steps: preparing raw materials, extracting raw materials, carrying out centrifugation, mixing, preparing tomato paste, mixing, grinding, mixing all raw materials, concentrating, and drying. The provided soup base has a sour and fresh taste, is palatable, is rich in nutrients, and has the functions of clearing heat, moistening the lung, resolving phlegm, relieving coughing, and freeing the orifices, if people eat the soup base for a long term.