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Wild tomato Solanum sitiens somatic embryo induction method and somatic embryo genetic transformation method

A technology of tomato and tomato, applied in the field of somatic embryo induction method and somatic embryo genetic transformation of wild tomato

Active Publication Date: 2013-09-25
ZHOUKOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, domestic and foreign researches on Solanaceae plants, especially tomato, mostly focus on conventional hybridization and molecular breeding of tomato, interaction mechanism between tomato plants and pathogenic bacteria, synthesis and metabolism of lycopene and tomatine, heat resistance and salt tolerance of tomato As well as tomato developmental molecular biology, etc., but there is no systematic report on the induction of somatic embryogenesis and somatic embryo genetic transformation system of the wild relative of tomato, Rigi solanum.

Method used

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  • Wild tomato Solanum sitiens somatic embryo induction method and somatic embryo genetic transformation method
  • Wild tomato Solanum sitiens somatic embryo induction method and somatic embryo genetic transformation method
  • Wild tomato Solanum sitiens somatic embryo induction method and somatic embryo genetic transformation method

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Embodiment 1

[0031] The present invention will be described in detail below in conjunction with Rigi solana example.

[0032](1) Aseptic seedling cultivation: select healthy and plump seeds, first sterilize the surface with 75% (v / v) ethanol for 30 seconds, rinse with sterile water 5 times; then treat with 2.5% ammonium hypochlorite for 8-10 minutes, without Rinse 3 times with bacterial water; put the sterilized seeds in a sterile 1.5ml centrifuge tube, germinate at 4°C for 4 days; then sow them in AGM (Accelerating germination medium, MS+3% sucrose+0.1mg / LGA3 +7.8g / L agar powder, pH5.8), incubate in the dark at 25°C, and germinate. Transfer the seeds with exposed radicle to the seedling medium SSCM1 (Seedlings culture medium1), as for 25°C, 16h light / 8h dark, the light intensity is 120μmol.m -2 the s -1 Cultivate under tissue culture conditions, and follow-up experiments can be carried out after the aseptic seedlings grow 3-4 true leaves.

[0033] (2) Three different explants of Solana...

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Abstract

The invention relates to the field of genetic transformation, specifically to a wild tomato Solanum sitiens somatic embryo induction method and a somatic embryo genetic transformation method. The induction method provided by the invention comprises the following steps: (1) culturing Solanum sitiens aseptic seedlings;(2) carrying out dark culture and inducing roots, stems (without axillalry buds) and leaves of Solanum sitiens on a embryogenic callus induction medium ECIM to generate embryogenic callus;(3) transferring the generated embryogenic callus onto a somatic embryogenesis induction medium SEIM in the light to induce somatic embryogenesis; and (4) inducing budding and seedling.

Description

technical field [0001] The invention relates to the field of genetic transformation, in particular to a method for inducing wild tomato solanum somatic embryos and a method for genetic transformation of somatic embryos. Background technique [0002] Tomato is a worldwide economic crop of the family Solanaceae. Wild tomato Riki solanum is a wild relative of modern cultivated tomato. Its natural growth conditions are special and its geographical distribution is narrow. It only exists in Chile and Peru in South America and is on the verge of extinction. However, its biological growth is huge and fast, especially its rich wild gene resources, which are valuable gene sources for modern tomato resistance, hybrid breeding and variety improvement. [0003] At present, domestic and foreign researches on Solanaceae plants, especially tomato, mostly focus on conventional hybridization and molecular breeding of tomato, interaction mechanism between tomato plants and pathogenic bacteria,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李成伟徐克东刘坤张怡张菊张福丽常云霞谭光轩黄晓晖吴曼曼
Owner ZHOUKOU NORMAL UNIV
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