72 results about "Tamarix arborea" patented technology

The invention discloses a bacillus atrophaeus strain DPPG-28 and an application in crop disease control, and belongs to the field of agricultural biotechnology. The strain is separated from soil of drip irrigation fields with damping off in tomato processing production areas, and has an accession number of CGMCC No. 9394. The strain can be used for biological control of crop pathogens such as rhizoctonia solani, pythium aphanidermatum, alternaria solani, phytophthora capsici, tomato scab fungi, and the like; the control effects on tomato damping off, capsicum early blight, and cotton damping off reach respectively 74.17%, 66.83%, and 70.12%; the strain can significantly improve the crop germination rate, plant height, fresh weight, and dry weight at the seedling stage. The strain of the invention has high-efficient wide-spectrum germicidal activity, and has wide application prospects in biocontrol agents.

The invention discloses production technology for extracting purified lycopene from tomato waste residue serving as a raw material, which comprises the following steps of: firstly, separating tomato skin from tomato seeds; secondly, extracting lycopene extract from the tomato skin by subcritical extraction technology; and finally, separating and purifying the lycopene in the lycopene extract by using a silica gel short column. The extraction ratio of the lycopene is 80 to 90 percent, and the lycopene content of extracted lycopene oleoresin can reach 6 to 50 percent.

The invention discloses a greenhouse cultivation method of pollution-free cherry tomatoes, and relates to the field of cherry tomato cultivation technologies. The greenhouse cultivation method mainly comprises the following steps of (1) seedbed preparation, (2) seed soaking and accelerating germination, (3) nutrient soil preparation and earthen bowl making, (4) sowing, (5) seedbed management, (6) seedling separating, temporary planting and management, (7) fix planting, (8) greenhouse management, (9) pest control and (10) harvesting. According to the growth characteristics of cherry tomatoes, the greenhouse cultivation method of the pollution-free cherry tomatoes is scientifically and reasonably made, efficient, low-toxicity and low-residue chemical pesticide is selected to control plant diseases and insect pests, so that high-quality pollution-free cherry tomatoes are produced, and product yield and quality are improved.

The invention provides a preparation method of a pink tomato material, and belongs to the field of plant gene engineering. The method comprises the following steps: 1, carrying out sequence analysis on the transcription factor S1MYB12 for controlling tomato skins to determine a specific sgRNA sequence; 2, constructing an expression vector containing sgRNA and a Cas9 protein; 3, transforming the obtained recombinant vector to red tomato through an Agrobacterium infection technology to obtain T0 generation transgenic tomato plants; and 4, planting the T0 generation plants with S1MYB12 gene sequence mutation in a greenhouse, selfing to obtain T1 generation plants, and screening plants free from exogenous transgenic components, with S1MYB12 gene sequence homozygous mutation and with pink fruits from the T1 generation plants in order to obtain the pink tomato material. The method has the advantages of low cost, preparation cycle shortening, and obtaining of the pink tomato material within one year; and the pink tomato does not contain exogenous gene, is safe and is free from risk.

The invention discloses a Siamese bacillus and an application of the same in controlling tomato root-knot nematode disease. The strain is identified as Bacillus siamensis LK0006 (CGMCC No. 15215). Theresults showed that the inhibition rate of nematodes is 90.35% when the fermentation broth is diluted 25 times and treated for 48 hours in vitro. The hatching of eggs could be inhibited by 25, 50, 100 times dilution of fermentation broth. Bacillus siamensis LK0006 wettable powder had a significant control effect on tomato root-knot nematode disease (75.02%) under pot culture condition, and tomatohad strong growth and no pesticide harm, so it had a good prospect in biocontrol of tomato root-knot nematode disease.

The invention discloses a method for breeding an inbred line of purple solanum lycopersicum, and belongs to the technical field of plant breeding. The method comprises the following steps of: 1) preparing a red F1-generation hybrid by using red and yellow solanum lycopersicum; 2) hybridizing the red F1-generation hybrid which serves as a male parent or a female parent and green solanum lycopersicum to obtain a three-way cross hybrid; 3) back-crossing the red three-way cross hybrid by using the green solanum lycopersicum as a male parent or a female parent to obtain a BC1 generation; 4) selecting a single green plant from a BC1-generation segregation population, and performing inbreeding to obtain a BC1F2-generation seed; 5) selecting the purple solanum lycopersicum from the BC1F2-generation seed for inbreeding seed reservation; and 6) performing inbreeding homozygosis of three to five generations to obtain the inbred line of the purple solanum lycopersicum. A novel breeding means is provided for germplasm resources of the purple solanum lycopersicum, can be used for breeding purple solanum lycopersicum hybrids with distinct advantages, and can be popularized and applied to breeding and production.

The invention provides a tomato protein SLMBP21 and a tomato gene SLMBP21 for coding the protein, wherein the nucleotide sequence of the gene is shown in SEQ ID No.1. The invention has the advantages that the tomato gene SLMBP21 belongs to a tomato abscission zone developmental gene in an MADS-box gene family, is capable of inhibiting the expression of the gene SLMBP21 so as to obviously influence the development of a tomato abscission zone to produce a transgenic plant with no tomato abscission zone or an incompletely developed abscission zone. The tomato protein SLMBP21 can be used for solving the problems of blossom drop and fruit drop of various crops, vegetables, fruits and flowers and the problem of industrial picking of tomatoes; moreover, different form the situation that the development of inflorescence in a JOINTLESS mutant is affected, the development of inflorescence in the SLMBP21transgenic plant is not affected, which means that the gene SLMBP21 has higher application value.

The invention discloses a method for planting an early-matured spring tomato, comprising the steps as follows: (1) preparing a soil conditioner; (2) preparing a special insecticide; (3) facility conditions; (4) seed selection and seed treatment; (5) cultivating strong seedlings; (6) field planting; (7) cultivation and management; and (8) harvesting. By adopting the method for planting an early-matured spring tomato provided by the invention, the major problem concerning soil and disease and insect control in the process of tomato planting is solved, and the quality and yield of tomato output are improved. The method is suitable for popularization and application in large-scale vegetable plantations.

The invention discloses a tomato planting method capable of preventing orobanche aegyptiaca parasitism on tomatoes. The tomato planting method mainly comprises the following steps that (a) seedlings are raised, wherein an early-maturing variety is selected for tomato seedling raising and planting, and soil, vermiculite, composted farmyard manure, a bacillus subtilis fungicide and a streptomyces pactum fungicide are selected as a matrix during tomato seedling raising; (b) transplantation is performed; (c) fertilizers are applied, wherein urea is applied three times in a dripping mode before flowering and in an initial fruit-bearing stage, wherein the urea concentration is 10-4 mol/L to 10-5 mol/L; (d) first time of watering begins in a dripping mode before flowering and is stopped before harvesting, and a spore suspension of bacillus subtilis and streptomyces pactum is added for 4-5 times in a dripping mode; (f) tomato stems and leaves are broken turned into tomato field piece soil for the depth of 18-23 cm after harvesting from the end of July to the beginning of August, corns are planted after 18-23 days, and the corns are Zhengdan 958. Compared with the prior art, the method has the advantages of being quick, convenient and efficient, capable of effectively preventing orobanche aegyptiaca parasitism and remarkably increasing yield and the like.

The invention relates to the field of genetic transformation, specifically to a wild tomato Solanum sitiens somatic embryo induction method and a somatic embryo genetic transformation method. The induction method provided by the invention comprises the following steps: (1) culturing Solanum sitiens aseptic seedlings;(2) carrying out dark culture and inducing roots, stems (without axillalry buds) and leaves of Solanum sitiens on a embryogenic callus induction medium ECIM to generate embryogenic callus;(3) transferring the generated embryogenic callus onto a somatic embryogenesis induction medium SEIM in the light to induce somatic embryogenesis; and (4) inducing budding and seedling.

The invention discloses a plant growth-promoting rhizobacteria strain which is separated from dung of bos grunniens in Qinghai Province and preserved in the General Microorganisms Collection Center of China Committee for Culture Collection of Microorganisms with a preservation name of Bacillus subtilis QM34 and a preservation number of CGMCC No. 2669 on September 22, 2008; the strain QM34 has antagonistic action on 12 pathogenic bacteria of soil-born plant fungus; and the fermentation solution of the strain QM34 and the cell-free nutrient solution of the fermentation solution of the strain QM34 has obvious prevention and treatment effects on alternaria solani, significantly promotes the growth of tomato plants and can be colonized in a large quantity according to the tomato plant rhizosphere. Therefore, the rhizobacteria strain is a broad-spectrum growth-promoting rhizobacteria strain used or preventing and treating alternaria solani and most popular among people.

The invention discloses a tomato early cultivation method. The tomato early cultivation method comprises the steps of (1) stubble arrangement, (2) nutrition bed soil preparation, (3) seeding, (4) seeding stage management (5) preparation before planting, (6) fertilizer planning and management, (7) soil preparation and ridging, (8) film selecting and covering, (9) seedling density selecting and (10) planting. In the step (1), autumn crops are directly seeded in July in a greenhouse in a two-film one-straw-mat cultivation mode, and seedling growing is conducted on early spring afterward crops in February of the next year. In the step (2), 25-30m<2> of seedling growing beds need to be prepared for every 668m<2> of planting area and bed soil accounts for 40%, soil accounts for 40% and cow dung which becomes decomposed by 70% to 80% accounts for 20%. In the step (3), potassium permanganate diluted by 1000 times is used for sterilizing summer and autumn crop seeds and copper sulfate diluted by 300 times is used for sterilizing winter crops and early spring crops. In the step (4), the seedling stage management periods of all the crops are 11.20-4.1 and 9-10, watering, thermal insulation and freezing prevention are conducted before winter. In the step (5) a mixture containing 100g of bacteriostatic agents and 15L of water is used for spraying seedlings 10 days before transplanting. In the step (6), if fertilization is planned according to the estimation that 10000kg fruits are produced in every 667m<2>, 38.6kg of pure nitrogen is needed and it is enough to maintain 19kg of the pure nitrogen in the soil. In the step (7), the tilling depth is 30 centimeters, the ridging width is 70 centimeters and the height is 10 centimeters. In the step (8), in the overwintering greenhouse, polyethylene three-layer composite purple light films and polyethylene anti-drop green films are used preferably. In the step (9), about 2900 plants are planted in the greenhouse every 667 square meters. In the step (10) for every 667 square meters, 12kg of CM solid are used and soaked in warm water at the temperature being 40 DEG C for 4-6 houses, the water is added for diluting and the mixed liquid is used for watering the seedling growing beds. The tomato early cultivation method is low in investment and greatly improves the economical benefits of peasants.

The present invention relates to a tomato genetic transformation method, belongs to the technical field of genetic engineering, and specifically discloses a whole tomato seed infection method. The method comprises the following steps: measuring a physiological imbibition curve of mature tomato seeds; culturing target gene-containing agrobacterium rhizogenes; transforming disinfected and sterilized tomato seeds into the agrobacterium rhizogenes liquid with an OD600 value of 0.8-1.3 through combining the physiological imbibition curve of the tomato seeds to carry out oscillation infection for 7-9 h; carrying out co-culture for 3 days under a dark condition and at a temperature of 25 DEG C; screening a resistant plant; carrying out rooting and cultivation on the resistant plant; and carrying out plant transforming, seedling exercising, transplantation and the like. According to the present invention, agrobacterium rhizogenes liquid infection is adopted at the tomato seed imbibition stage so as to substantially improve transformation efficiency; pressure culture medium screening is adopted to screen, such that an experimental period is shortened by 2-3 months, an experimental process is simplified, and identification workload after transformation is reduced; and important significances are provided for theoretical researches and genetic breeding practices of tomato genetic engineering.

The invention relates to the technical field of crop seed breeding using a gene editing technology, in particular to a method for innovatively preparing a new variety of TYLCV (tomato yellow leafcurlvirus)-resisting solanum lycopersicum by gene editing and application thereof. The method has the advantages that by editing a gene Ty-5, the recovery mechanism of the plant self on DBS (deep brain stimulation) is utilized, the deletion of DNA (deoxyribonucleic acid) sequence is introduced, the function of the gene Ty-5 is lost, and the disease-resistant property on TYLCV is obtained; compared with the conventional seed breeding method, the resistance on the TYLCV by the disease sensing material can be quickly obtained; compared with the conventional seed breeding method, the garden characterof the receptor material can be well reserved.

The invention relates to a method for in-situ generation addition and rapid breeding of solanum lycopersicum. The method comprises the following steps: a hole tray is used for growing seedlings, and solanum lycopersicum seedlings growing to four true leaves are obtained; the solanum lycopersicum seedlings are planted in the field to grow outdoors; during the growth of the solanum lycopersicum seedlings, lateral branches are cut, only main trunks are left, and water and fertilizer are controlled; when first moss flower of solanum lycopersicum plants buds, topping is performed; when the first moss of the solanum lycopersicum sets fruits, cutting is performed, and 1-2 fruits are left; during the 1-2 d after fruit color transformation in summer, fruit harvesting and seed taking are performed,fruit harvesting and seed taking are performed immediately after color transformation in winter, and seeds are directly planted into the hole tray for the next generation of seedling growing after seed cutting. The method for adding generations and breeding rapidly of the solanum lycopersicum in situ can realize three or four generations of continuous breeding a year.