75 results about "Pythium aphanidermatum" patented technology

The invention discloses a sterilizing composite containing captan. The sterilizing composite comprises an effective and active component, wherein the effective and active component of the sterilizing composite consist of captan as a first active component and methoxyl acrylate bactericide from pyraclostrobin, trifloxystrobin and coumoxystrobin as a second active component; the weight ratio of the first active component to the second active component is 1:10-20:1, and preferred as 1:5-10:1. The sterilizing composite can be prepared into an agriculturally-allowable suspending agent, a suspending emulsion, a suspending seed coating agent, a micro-capsule suspending-suspending agent and seed-treatment dispersible powder. The sterilizing composite disclosed by the invention has the advantages that the components are reasonable, the sterilizing effect is good, the medicine use cost is low, and the activity and the sterilizing effect are not in simple superposition of various components. Compared with the traditional single preparation, the sterilizing composite disclosed by the invention not only has an obvious sterilizing effect, but also has an obvious synergistic action, has no pollution to crops, is good in safety, meets the requirement of safety of pesticide formulations, and has obvious preventing effects on wheat powdery mildew, rice sheath blight diseases, cochliobolus miyabeanus, damping off, vegetable pythium aphanidermatum and downy mildew.

The invention provides a recombinant mortierella alpina strain Ma-oPaFADS17 with heterologous expression coming from pythium aphanidermatum Omega-3 desaturase genes and a method for constructing the mortierella alpina strain with heterologous expression coming from pythium aphanidermatum Omega-3 desaturase genes. The method adopts mortierella alpine uracil auxotrophic strain CCFM 501 as materials and uses an agrobacterium tumefaciens mediated genetic manipulation technology to obtain a recombinant strain with a high yield of EPA in the environment of normal temperature, wherein the yield of EPA reaches 617.1 mg / L, and the content of the EPA accounts for 18.7 percent of the total amount of fatty acid, which has an important meaning for the basic research and product development of oil-producing fungus mortierella alpine.

The invention relates to a novel rhizobiumleguminosarum strain, wherein the strain has high biological nitrogen fixation activity, has certain preventive and therapeutic effects on fungal diseases of plants and has good saline-alkaline resistance and high temperature resistance, so that the strain has functions of nitrogen fixation, disease prevention, stress resistance and yield increase. The rhizobiumleguminosarum is preserved in the China General Microbiological Culture Collection Center Committee on July 15, 2013 with a preservation number of CGMCCNo.7924. As shown in researches, the strain can nodulate on peanut roots and the strain has a good inhibitory effect on botrytis cinerea and pythium aphanidermatum and has saline-alkaline resistance and high temperature resistance.

The invention relates to a 4-substituted phenyl-6-trifluoromethyl-2-amino pyrimidinamine compound which has a novel chemical structure and a general formula III, a synthesis method for the compound and the application of the compound used as bacteriacide. The method comprises the following steps of: reacting substituted phenyl methyl ketone used as an initial raw material and trifluoroacetic acid ethyl ester under catalysis of sodium alcoholate to generate 4,4,4-trifluoro-1-substituded phenylbutane-1,3-diketone, and reacting 4,4,4-trifluoro-1-substituded phenylbutane-1,3-diketone and guanidinium salt under an alkaline condition to generate a III series compound with the general formula III. The compound with the general formula III can inhibit growth of pathogenic bacteria such as rice sheath blight, rice bakanae disease, exserohilum turcicum, bipolaris maydis, curvulavia lunata, corn kernel rot, corn top rot pathogenic bacteria, fusarium graminearum, cotton anthracnose, bean fusarium, rape sclerotinia rot, botrytis cinerea, cucumber fusarium wilt pathogenic bacteria, cucumber brown rot pathogenic bacteria, cucumber scab pathogenic bacteria, hot pepper root rot pathogenic bacteria, kidney bean anthracnose bacteria, and pythium aphanidermatum. In the formula III, R is methyl, ethyl, methoxy group, fluorine, chlorine, bromine, trifluoromethyl and other groups, and the number of R is 1 to 3.

The invention discloses a rapid immunoassay test strip for phytophthora infestans and application thereof. The test strip comprises a bottom plate, wherein a water absorption pad, a detection pad, a gold label pad and a sample pad are sequentially adhered to one surface of the bottom plate from top to bottom; every two adjacent pads are connected in an overlapped mode at the joint, a nitrocellulose membrane serves as a base pad of the detection pad, a quality control line and a detection line are transversely arranged on the nitrocellulose membrane from top to bottom, the detection line is located below the quality control line, the detection line is coated with an anti-pyricularia grisea polyclonal antibody 0550, and the quality control line is coated with goat anti-mouse IgG. The gold label pad is sprayed with a nanogold labeled anti-pyricularia oryzae monoclonal antibody. The specificity is high, and no cross reaction is caused to pythium aphanidermatum, pear rust water, pear rot, pear anthracnose and the like of Asian pears; the sensitivity of the test strip can reach 1*10<5> cfu / mL, and the test strip can be used for rapid screening of pythium aphanidermatum in fields.

The invention discloses uses of clove bud volatile oil, particularly applications of clove bud volatile oil in prevention and control of root rot of Chinese medicinal materials or crops in agricultureor applications of clove bud volatile oil in prevention and control of pathogenic bacteria causing diseases in the aerial parts of Chinese medicinal materials or crops. According to the present invention, the experiment results show that the clove bud volatile oil can inhibit the mycelial growth of four main pathogenic bacteria causing Panax notoginseng root rot and comprising Fusarium oxysporum,Fusarium solani, Pythium aphanidermatum and Cylindrocarpon destructans, can completely inhibit the mycelial growth of three pathogenic bacteria causing the diseases in the aerial parts of Panax notoginseng and comprising Botrytis cinerea, Colletotrichum gloeosporioides and Rhizoctonia solani, and has the antibacterial effect equivalent to the antibacterial effect of the chemical pesticide hymexazol, wherein the compounding of clove bud and hymexazol can provide synergistic effect for a variety of strains; the prevention and control effect on Panax notoginseng blade gray mold by the clove budvolatile oil is up to 60.77%; and the volatile oil has characteristics of easy volatility and decontamination through aroma, such that the fertilizers or pesticides developed from the volatile oil have advantages of safety, effectiveness, low residue and the like.

The invention discloses a sterilizing composite containing captan. The sterilizing composite comprises an effective and active component, wherein the effective and active component of the sterilizing composite consist of captan as a first active component and methoxyl acrylate bactericide from pyraclostrobin, trifloxystrobin and coumoxystrobin as a second active component; the weight ratio of the first active component to the second active component is 1:10-20:1, and preferred as 1:5-10:1. The sterilizing composite can be prepared into an agriculturally-allowable suspending agent, a suspending emulsion, a suspending seed coating agent, a micro-capsule suspending-suspending agent and seed-treatment dispersible powder. The sterilizing composite disclosed by the invention has the advantages that the components are reasonable, the sterilizing effect is good, the medicine use cost is low, and the activity and the sterilizing effect are not in simple superposition of various components. Compared with the traditional single preparation, the sterilizing composite disclosed by the invention not only has an obvious sterilizing effect, but also has an obvious synergistic action, has no pollution to crops, is good in safety, meets the requirement of safety of pesticide formulations, and has obvious preventing effects on wheat powdery mildew, rice sheath blight diseases, cochliobolus miyabeanus, damping off, vegetable pythium aphanidermatum and downy mildew.

The invention discloses endophytic antagonistic Serratia sp. ZF05 from Fenghuang Dancong tea plants and an application thereof to biocontrol. Sampling from Fenghuang Dancong tea plant leaves, separation and purification, screening and cultivation are carried out in order to obtain a strain of Serratia sp. ZF05 GDMCC No:60015 with good biocontrol effects. The bacterial strain is obviously different from common Serratia sp. in the aspects of physiological-biochemical characteristics and molecular level, so the bacterial strain is a typical new bacterial strain. The bacterial strain ZF05 has good inhibition effects for Alternaria alternate, Walnut blight, Alternaria sp., Rhizoctonia solani, and Pythium aphanidermatum; mycelium growth inhibition rates are as follows: 73.02%, 73.70%, 78.51%, 84.56% and 20.06%; Serratia sp. ZF05 is a biocontrol bacterium with obvious typicality and high specificity, and has good application prospects for developing new biopesticides or biological control microbial inoculum; Serratia sp. ZF05 has wide application values for biocontrol.

The invention discloses an application of a castor cake meal coarse extract in inhibition on plant pathogenic pythium. The plant pathogenic pythium includes Pythium ultimum, Pythium aphanidermatum and Pythium arrhenomanes. The castor cake meal coarse extract is prepared through the steps of weighing castor cake meal powder which is crushed and passes through a sieve in 80-120 meshes; adding distilled water, performing reflux extraction and filtering the liquid while hot and collecting a filtrate with medicine residue for later use; adding distilled water to the medicine residue, continuously performing the reflux extraction, filtering the liquid while hot and collecting a filtrate; mixing the two filtrates, and evaporating and concentrating the mixed filtrate to obtain the coarse extract. The invention not only provides an excellent novel bactericide for production but also develops a new approach of utilization of waste in castor industry.