12 results about "Pythium aphanidermatum" patented technology

The present invention provides a method for detecting fungal DNA in a turf grass sample with a loop-mediated isothermal amplification (LAMP) assay which contains primers for fungal DNA of at least one turf pathogenic fungi selected from Sclerotinia homoeocarpa, Rhizoctonia solani spp., Pythium aphanidermatum, Gaeumannomyces graminis spp., Microdochium nivale spp., Magnaporthe poae, Colletotrichum graminicola, Colletotrichum cereale and Pythium ultimum var. ultimum, comprising: subjecting the turf sample to a LAMP reaction wherein the LAMP reaction uses a primer set of four or more nucleic acid sequences with each primer in the set having from 15 to 50 nucleic acids The primers useful in the present method are selected from specifically selected internal transcribed spacer regions or genes of the target fungi to provide improved assay results.

The invention discloses a brevibacterium casein peptide A derivative compound and application thereof, the brevibacterium casein peptide A derivative is a natural compound generated by brevibacterium brevis B011, the structural general formula of the brevibacterium casein peptide A derivative is cyclo-(DFPLDYNQYVOL), the mass-to-charge ratio m / z of the brevibacterium casein peptide A derivative is 1236.6789, and the accurate molecular weight of the brevibacterium casein peptide A derivative is 1235.6745. The derivative is purified through semi-preparative liquid chromatography, and a plate bacteriostasis test result shows that the minimum inhibitory concentration of the derivative to valsa bergamot pear germs is lower than that of tyrocidine A, so that the derivative has good bacteriostatic activity and application and development prospects. The invention also discloses a method for exploring the derivative compound.

The application discloses a primer composition for detecting Pythium sylvesterianum based on LAMP technology and application thereof, and belongs to the technical field of microorganism detection; the primer composition is a LAMP primer composition designed according to a specific sequence shown in SEQ ID NO. 1. The application adopts a method for rapidly detecting Pythium sylvesterianum, the pathogenic bacterium of stem base rot of raw ginger, based on loop-mediated isothermal amplification technology, and establishes a perfect detection and field real-time detection system for soil samples, water source samples and diseased plant samples, thereby opening up a new train of thought and method for the measurement and prediction of field crop diseases, winning valuable time for the prevention and control of related diseases, and reducing the loss caused by diseases to a certain extent.

The present application relates to a kind of Trichoderma fermentation liquor and preparation method thereof.The Trichoderma fermentation liquor is the fermentation liquor of Trichoderma sulphureum DH4-3, and the preservation number of Trichoderma sulphureum DH4-3 is CGMCC No.40704.The preparation method of the above-mentioned Trichoderma fermentation liquor includes the following steps: Trichoderma sulphureum DH4-3 is inoculated into culture medium and fermented.The Trichoderma fermentation liquor has inhibitory effect on 12 kinds of tested plant pathogenic fungi, such as poplar bark rot fungus, nursery wilt fungus, blueberry branch wilt fungus, apple rot fungus, cucumber sclerotium blight fungus, apple fruit rot fungus, melon vine cutting fungus, rice malignant worm fungus, red bean anthracnose fungus, tobacco brown spot fungus, tobacco target spot fungus and Pythium aphanidermatum, and has the advantages of good control effect, wide inhibitory spectrum and wide application range.

The present application relates to the technical field of agricultural microbial agents, and specifically discloses a staphylococcus warneri agent for inhibiting pythium aphanidermatum, a preparation method and application thereof. The staphylococcus warneri agent comprises: staphylococcus warneri bacterial slurry and slow-release adjuvant mixed at a mass ratio of 1:1-3 to form granules; the staphylococcus warneri bacterial slurry comprises staphylococcus warneri bacterial liquid and dry protective agent, the solid content of the staphylococcus warneri bacterial liquid is 60-80%, and the mass fraction of the dry protective agent is 1-10% of the mass fraction of the staphylococcus warneri bacterial liquid; the slow-release adjuvant comprises carrier adjuvant, slow-release adjuvant and trace elements mixed at a mass ratio of 6-8:1.5-3:0.5-1. The staphylococcus warneri agent has a significant effect on inhibiting pythium aphanidermatum, can significantly reduce the harm of pythium aphanidermatum to various crops, and can be used as an efficient and environmentally friendly agricultural disease control method.

The present invention relates to a method for controlling or preventing an infestation of useful plants by a phytopathogenic microorganism selected from the group consisting of Fusarium pseudograminearum, Fusarium culmorum, Fusarium oxysporum, Fusarium graminearum, Bakania oryzae, Sporidesmium humicola, Botrytis cinerea, Phytophthora sojae, Phytophthora capsici, Phytophthora infestans, Pythium aphanidermatum, Pythium arrhenomanes, Pythium deliense, Puccinia striiformis, Puccinia hordei, Puccinia malvaccarum, Pseudoperonospora cubensis and Sphaerotheca fuliginea, which comprises the application of a compound of formula (I) to useful plants, to the locus thereof, or to their propagation material.

This invention belongs to the fields of organic synthesis and pesticide application, and specifically relates to the application of an indole / indazole compound with an imine fragment, the structural formula of which is as follows: This invention tested the indole / indazole compounds with the imine fragment in vitro against *P. capsici*, *P. aphaneridermatum*, and *P. litchii*. It was found that compounds A1 and A5 exhibited excellent activity against oomycetes, particularly against *P. capsici*, with EC50 values ​​of 4.4 μg / mL and 2.6 μg / mL, respectively, similar to the positive control metalaxyl (2.3 μg / mL). It is also noteworthy that when compounds A1-A5 are prepared as Cu... 2+ When the ionic complex B1-B5 was used, its inhibitory effect on oomycetes was significantly enhanced. Complex B1 showed excellent in vivo protective and curative effects against Phytophthora capsici on pepper leaves, with control efficiencies of 91.45% and 83.75% at a concentration of 200 μg / mL, respectively, which were superior to metalaxyl (80.05% and 76.15%).

The application belongs to the field of microorganism and genetic engineering technology, and particularly relates to a chitinase mutant and application thereof in promoting plant growth. The wild-type chitinase is subjected to directed evolution in vitro through error-prone PCR technology, and a chitinase mutant with significantly improved enzyme activity and environmental stability is obtained. The enzyme activity of the mutant at 50 DEG C and pH 7 is 1051.36 U / mL, which is about 2.7 times of that of the wild-type chitinase (385.45 U / mL). The mutant has good killing effect on pests such as pests of Lepidoptera, Thysanoptera and Coleoptera, and also has good bacteriostatic activity on pathogenic bacteria such as Pythium aphanidermatum, Pythium ultimum, Botrytis cinerea, Phytophthora infestans and Curvularia inaequalis. When used in the growth and development of vegetable plants or food plants, the mutant can promote plant growth and significantly improve the yield and quality of plants.

This invention discloses a rice damping-off disease fungicide and method, relating to the field of rice fungicide technology. It includes a movable base plate, with a damping-off disease agent storage tank bolted to the upper surface of the base plate. A spraying mechanism is installed on one side of the base plate, and a driving mechanism is installed on the lower surface. The driving mechanism includes mounting plates fixedly connected to the four corners of the lower surface of the base plate. Through the design of stabilizing mechanisms and other structures, this invention enables the movable base plate to be pushed by movable wheels, causing a rotating rod to rotate. This, in turn, causes a push plate to move a drive toothed plate, resulting in two spray pipes making counter-clockwise circular motions. This, in turn, drives the atomizing nozzles to move synchronously, effectively fungizing the rice paddy and treating rice damping-off disease. The spray pipes spray a fan-shaped area in front of them by pushing the movable base plate, avoiding the need for manual arm swinging to spray the area.

The application discloses a tobacco biocontrol bacterium, a biocontrol bacterium agent and a propagation method thereof, the bacterium is a strain of Sphingobium sp. PE05 with a preservation number of CCTCC No: M20221104; the strain of Sphingobium sp. PE05 is used for preventing and treating black shank and / or antagonizing Pythium aphanidermatum. The bacterium is separated from healthy tobacco leaves of a cigar tobacco variety of Yunnan Province Puer City Jiangcheng County Yunxue No. 2 and identified as Sphingobium sp., the bacterium is not used for preventing and treating black shank and related bacteria, and the bacterium is not used for antagonizing Pythium aphanidermatum; experiment verification shows that the inhibition rate of the bacterium to Pythium aphanidermatum mycelium growth is as high as 73.5%, the bacterium propagation product has a prevention effect on tobacco black shank of more than 80%, and the bacterium propagation product has the characteristics of low use cost, no residue and safety to human and livestock.

The application discloses a Pseudomonas aeruginosa, which is Pseudomonas aeruginosa EB-Q03-02, and the preservation number is CGMCC No.30779; and the application of the Pseudomonas aeruginosa in preventing and treating Graymold, Pythium aphanidermatum and tobacco blight. The Pseudomonas aeruginosa EB-Q03-02 is separated from the root system of healthy passion fruit, has antagonistic effect on the passion fruit blight, and has obvious antagonistic effect on the passion fruit blight caused by tobacco blight. Further, the Pseudomonas aeruginosa EB-Q03-02 can effectively inhibit the Graymold, Pythium aphanidermatum and tobacco blight, and the inhibition rate is as high as 81% or more.

The application belongs to the technical field of biology, and specifically discloses a Serratia plymuthica R72, which is preserved in the China Center for Type Culture Collection, and the preservation date is February 2, 2026, and the preservation number is CCTCC M 2026311; can be applied to the prevention and treatment of atractylodes rhizome root rot, and the prevention and treatment effect on the main pathogenic bacteria of atractylodes rhizome root rot, i.e., Fusarium oxysporum, is 89.3% in a plate confrontation test; meanwhile, the Serratia plymuthica R72 provided by the application has strong antagonistic effects on the pathogenic bacteria of rice false smut, sclerotinia sclerotiorum, sclerotinia sclerotiorum, tomato gray mold, melon fruit rot, brassica nigra, rice blast, rice sesame leaf spot, and tomato wilt, and the inhibition rate is more than 88%.