23 results about "Pythium aphanidermatum" patented technology

The invention discloses a multifunctional talaromyces sp. Fungus RB-2 for preventing and controlling ginger stem rot and application of the multifunctional talaromyces sp. Fungus RB-2, and belongs to the technical field of microorganisms. The talaromyces sp. Fungus RB-2 is preserved in the China Center for Type Culture Collection, the preservation number of the talaromyces sp. Fungus RB-2 is CCTCC NO: M 20251722, and the preservation date of the talaromyces sp. Fungus RB-2 is July 28, 2025. The talaromyces sp. Strain RB-2 with the effect of inhibiting the growth of pathogenic bacteria is separated and purified, and the strain RB-2 has remarkable antagonistic activity on ginger stem rot pathogenic bacterium group pythium aphanidermatum and various economic crop pathogenic bacteria and has the potential of being developed into a biocontrol inoculant. The talaromyces sp. Strain RB-2 also has phosphorus dissolving and growth promoting capabilities, can remarkably promote the growth of ginger, and has the potential of preparing a soil conditioner and a microbial fertilizer, so that the micro-ecology of soil is improved, and the productivity of crops is enhanced.

The invention relates to the technical field of agricultural microbial agents, and particularly discloses a staphylococcus warneri microbial agent for inhibiting pythium aphanidermatum as well as a preparation method and application of the staphylococcus warneri microbial agent. The staphylococcus warneri bacterial agent is prepared by mixing staphylococcus warneri bacterial slurry and a sustained-release auxiliary material according to a mass ratio of 1: (1-3) and granulating, the staphylococcus warneri bacterial slurry comprises staphylococcus warneri bacterial liquid and a drying protective agent, the solid content of the staphylococcus warneri bacterial liquid is 60-80%, and the mass of the drying protective agent is 1-10% of the mass fraction of the staphylococcus warneri bacterial liquid; the slow-release auxiliary material is prepared by mixing a carrier auxiliary material, a slow-release auxiliary material and trace elements in a mass ratio of (6-8): (1.5-3): (0.5-1). The staphylococcus warneri bacterial agent has a remarkable inhibition effect on pythium aphanidermatum, can remarkably reduce the harm of pythium aphanidermatum to various crops, and can be used as an efficient and environment-friendly agricultural disease control means.

The present invention provides a method for detecting fungal DNA in a turf grass sample with a loop-mediated isothermal amplification (LAMP) assay which contains primers for fungal DNA of at least one turf pathogenic fungi selected from Sclerotinia homoeocarpa, Rhizoctonia solani spp., Pythium aphanidermatum, Gaeumannomyces graminis spp., Microdochium nivale spp., Magnaporthe poae, Colletotrichum graminicola, Colletotrichum cereale and Pythium ultimum var. ultimum, comprising: subjecting the turf sample to a LAMP reaction wherein the LAMP reaction uses a primer set of four or more nucleic acid sequences with each primer in the set having from 15 to 50 nucleic acids The primers useful in the present method are selected from specifically selected internal transcribed spacer regions or genes of the target fungi to provide improved assay results.

The present application relates to a kind of new compound and its preparation method and application, specifically 1-substituted phenyl-5-methyl-1 H Preparation and application of pyrazole-3-formamide derivatives. The structural formula of the compound 1-substituted phenyl-5-methyl-1 H Pyrazole-3-formamide derivatives is as shown in formula I. Wherein, R1 selected from: 4-chloro or 2,4-dichloro;R2 selected from: H, methyl, methoxy, cyclopropyl;R3 selected from: 3-methylphenyl, 3-nitrobenzyl, 4-nitrobenzyl, 4-chlorophenyl, 2-(5-chloro-1 H Indole-3-yl) ethyl, N-(4-chlorophenyl)-2-phenyl. Test shows that the compound of the application shows good antifungal activity to grape cavity fungus (Botryosphaeria dothidea) (Botryosphaeria dothidea), Pythium aphanidermatum (Pythium aphanidermatum), Fusarium oxysporum (Fusarium oxysporum), Gray pear spot fungus (Gymnosporangium japonicum), Alternaria alternata (Alternaria alternata), Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), Botrytis cinerea (Botrytis cinerea), and has wide application prospect. Botryosphaeria dothidea Pythium aphanidermatum Fusarium oxysporum Phyricularia grisea Alternaria nees Sclerotinia sclerotiorum Botrytis cinerea ​​​​​​​

The invention discloses a brevibacterium casein peptide A derivative compound and application thereof, the brevibacterium casein peptide A derivative is a natural compound generated by brevibacterium brevis B011, the structural general formula of the brevibacterium casein peptide A derivative is cyclo-(DFPLDYNQYVOL), the mass-to-charge ratio m / z of the brevibacterium casein peptide A derivative is 1236.6789, and the accurate molecular weight of the brevibacterium casein peptide A derivative is 1235.6745. The derivative is purified through semi-preparative liquid chromatography, and a plate bacteriostasis test result shows that the minimum inhibitory concentration of the derivative to valsa bergamot pear germs is lower than that of tyrocidine A, so that the derivative has good bacteriostatic activity and application and development prospects. The invention also discloses a method for exploring the derivative compound.

The application discloses a primer composition for detecting Pythium sylvesterianum based on LAMP technology and application thereof, and belongs to the technical field of microorganism detection; the primer composition is a LAMP primer composition designed according to a specific sequence shown in SEQ ID NO. 1. The application adopts a method for rapidly detecting Pythium sylvesterianum, the pathogenic bacterium of stem base rot of raw ginger, based on loop-mediated isothermal amplification technology, and establishes a perfect detection and field real-time detection system for soil samples, water source samples and diseased plant samples, thereby opening up a new train of thought and method for the measurement and prediction of field crop diseases, winning valuable time for the prevention and control of related diseases, and reducing the loss caused by diseases to a certain extent.

The present application relates to a kind of Trichoderma fermentation liquor and preparation method thereof.The Trichoderma fermentation liquor is the fermentation liquor of Trichoderma sulphureum DH4-3, and the preservation number of Trichoderma sulphureum DH4-3 is CGMCC No.40704.The preparation method of the above-mentioned Trichoderma fermentation liquor includes the following steps: Trichoderma sulphureum DH4-3 is inoculated into culture medium and fermented.The Trichoderma fermentation liquor has inhibitory effect on 12 kinds of tested plant pathogenic fungi, such as poplar bark rot fungus, nursery wilt fungus, blueberry branch wilt fungus, apple rot fungus, cucumber sclerotium blight fungus, apple fruit rot fungus, melon vine cutting fungus, rice malignant worm fungus, red bean anthracnose fungus, tobacco brown spot fungus, tobacco target spot fungus and Pythium aphanidermatum, and has the advantages of good control effect, wide inhibitory spectrum and wide application range.

The application provides an anti-disease promoting Aspergillus ochraceus AF-P9 and an application thereof, and belongs to the technical field of microorganisms.The application provides the Aspergillus ochraceus AF-P9, which has salt tolerance and alkali tolerance, has the ability of producing iron carrier and secreting chitinase, has a high efficient inhibiting effect on multiple pathogenic bacteria such as Fusarium oxysporum, Fusarium acuminatum, Fusarium decemcellulare, Pythium aphanidermatum, Pseudomonas syringae pv., Sclerotinia sclerotiorum and Phytophthora, and can be applied to the prevention and treatment of rice damping-off.Further, metabolites of the Aspergillus ochraceus AF-P9 are analyzed, and it is found that the Aspergillus ochraceus AF-P9 can produce tricyclic cyclohexanone compounds, cyclohexene compounds with hydroxyl, phenanthrene ketone compounds with hydroxyl, benzene ring compounds with two long chain esters and cyclohexene compounds with four annular structures.The Aspergillus ochraceus AF-P9 provided by the application provides a strain resource for the prevention and treatment of disease organisms.

The invention discloses a corn pythium stem rot resistance phenotype accurate identification method based on adaptive gene mining, and particularly relates to the field of corn disease resistance identification and gene mining, and the method comprises the following steps: S1, selecting a corn material to be identified, planting the selected corn material in a greenhouse, and adopting a unified seedling culture substrate and cultivation management measures to ensure that the growth state of corn seedlings is consistent; step S2, inoculating a strong pathogenicity pythium miscanthus or fusarium graminearum strain on a PDA plate culture medium with the diameter of 9 mm and containing 20 mL of sterilized bacteria, carrying out dark culture at 25 DEG C for 5-7 days, and preparing to inoculate when hyphae are fully distributed on the surface of PDA; s3, phenotype data collection is conducted on the 3th day, the 7th day, the 10th day and the 14th day after inoculation. According to the method, strict requirements of accurate gene mining on phenotypic data stability are met, disease-resistant gene omission caused by index one-sidedness is avoided, and comprehensiveness and accuracy of adaptive gene mining are remarkably improved.

The present application relates to the field of microorganisms, and particularly to a broad-spectrum antagonistic bacterium Bacillus velezensis SJD33 and application thereof. The present application provides Bacillus velezensis SJD33, which has a preservation number of CGMCC No. 29456. The strain has a significant inhibitory effect on Pythium aphanidermatum, Penicillium expansum, Alternaria alternata, Diplodia natalensis, Botrytis cinerea, Colletotrichum gloeosporioides and other various fruit pathogens (Valsa mali, Monilinia fructicola and Diplodia natalensis). SJD33 can significantly reduce the natural rot rate of pears; SJD33 can rapidly colonize in the wound of fruits; SJD33 does not affect the quality of fruits, and can delay the decrease of fruit hardness and L-ascorbic acid; the secondary metabolites of SJD33 are stable in activity, and have great application potential. The strain provides a new strain resource for the fruit disease biocontrol resource library.

The present application relates to the technical field of agricultural microbial agents, and specifically discloses a staphylococcus warneri agent for inhibiting pythium aphanidermatum, a preparation method and application thereof. The staphylococcus warneri agent comprises: staphylococcus warneri bacterial slurry and slow-release adjuvant mixed at a mass ratio of 1:1-3 to form granules; the staphylococcus warneri bacterial slurry comprises staphylococcus warneri bacterial liquid and dry protective agent, the solid content of the staphylococcus warneri bacterial liquid is 60-80%, and the mass fraction of the dry protective agent is 1-10% of the mass fraction of the staphylococcus warneri bacterial liquid; the slow-release adjuvant comprises carrier adjuvant, slow-release adjuvant and trace elements mixed at a mass ratio of 6-8:1.5-3:0.5-1. The staphylococcus warneri agent has a significant effect on inhibiting pythium aphanidermatum, can significantly reduce the harm of pythium aphanidermatum to various crops, and can be used as an efficient and environmentally friendly agricultural disease control method.

The present invention relates to a method for controlling or preventing an infestation of useful plants by a phytopathogenic microorganism selected from the group consisting of Fusarium pseudograminearum, Fusarium culmorum, Fusarium oxysporum, Fusarium graminearum, Bakania oryzae, Sporidesmium humicola, Botrytis cinerea, Phytophthora sojae, Phytophthora capsici, Phytophthora infestans, Pythium aphanidermatum, Pythium arrhenomanes, Pythium deliense, Puccinia striiformis, Puccinia hordei, Puccinia malvaccarum, Pseudoperonospora cubensis and Sphaerotheca fuliginea, which comprises the application of a compound of formula (I) to useful plants, to the locus thereof, or to their propagation material.

This invention belongs to the fields of organic synthesis and pesticide application, and specifically relates to the application of an indole / indazole compound with an imine fragment, the structural formula of which is as follows: This invention tested the indole / indazole compounds with the imine fragment in vitro against *P. capsici*, *P. aphaneridermatum*, and *P. litchii*. It was found that compounds A1 and A5 exhibited excellent activity against oomycetes, particularly against *P. capsici*, with EC50 values ​​of 4.4 μg / mL and 2.6 μg / mL, respectively, similar to the positive control metalaxyl (2.3 μg / mL). It is also noteworthy that when compounds A1-A5 are prepared as Cu... 2+ When the ionic complex B1-B5 was used, its inhibitory effect on oomycetes was significantly enhanced. Complex B1 showed excellent in vivo protective and curative effects against Phytophthora capsici on pepper leaves, with control efficiencies of 91.45% and 83.75% at a concentration of 200 μg / mL, respectively, which were superior to metalaxyl (80.05% and 76.15%).

The application belongs to the field of microorganism and genetic engineering technology, and particularly relates to a chitinase mutant and application thereof in promoting plant growth. The wild-type chitinase is subjected to directed evolution in vitro through error-prone PCR technology, and a chitinase mutant with significantly improved enzyme activity and environmental stability is obtained. The enzyme activity of the mutant at 50 DEG C and pH 7 is 1051.36 U / mL, which is about 2.7 times of that of the wild-type chitinase (385.45 U / mL). The mutant has good killing effect on pests such as pests of Lepidoptera, Thysanoptera and Coleoptera, and also has good bacteriostatic activity on pathogenic bacteria such as Pythium aphanidermatum, Pythium ultimum, Botrytis cinerea, Phytophthora infestans and Curvularia inaequalis. When used in the growth and development of vegetable plants or food plants, the mutant can promote plant growth and significantly improve the yield and quality of plants.

The invention discloses a plant nutrition sterilization suspension agent prepared based on natural substances, and particularly relates to the technical field of plant nutrition sterilization, the plant nutrition sterilization suspension agent comprises the following raw materials: a plant mixed extract, a plant ash leachate, a mangosteen shell extract, trace elements, citric acid, hibiscus manihot, animal waste and lotus pond sludge, and the balance of deionized water. The plant mixed extract contains mimosa pudica, celastrus angulatus, garlic, mint, marigold, clove, tea leaves and other raw materials, has a relatively high inhibition effect on wheat powdery mildew, cotton rhizoctonia solani, cucumber angular leaf spot, tobacco mosaic, pythium aphanidermatum and cucumber colletotrichum gloeosporioides, and has the inhibition rate of about 95% on botrytis cinerea and leaf mold of tomatoes; according to the present invention, the antibacterial agent has characteristics of no toxicity, no toxicity and high safety, and the mangosteen shell extract contains more flavonoids and polyphenols, such that the antibacterial effect of the plant can be improved, and the nutritional effect can be provided for the plant.

This invention discloses a rice damping-off disease fungicide and method, relating to the field of rice fungicide technology. It includes a movable base plate, with a damping-off disease agent storage tank bolted to the upper surface of the base plate. A spraying mechanism is installed on one side of the base plate, and a driving mechanism is installed on the lower surface. The driving mechanism includes mounting plates fixedly connected to the four corners of the lower surface of the base plate. Through the design of stabilizing mechanisms and other structures, this invention enables the movable base plate to be pushed by movable wheels, causing a rotating rod to rotate. This, in turn, causes a push plate to move a drive toothed plate, resulting in two spray pipes making counter-clockwise circular motions. This, in turn, drives the atomizing nozzles to move synchronously, effectively fungizing the rice paddy and treating rice damping-off disease. The spray pipes spray a fan-shaped area in front of them by pushing the movable base plate, avoiding the need for manual arm swinging to spray the area.

The invention belongs to the technical field of microorganisms and genetic engineering, and particularly relates to a chitinase mutant and application thereof in promoting plant growth. According to the present invention, by using the error-prone PCR technology, the direct evolution is performed on the wild type chitinase in vitro so as to obtain the chitinase mutant with significantly improved enzyme activity and significantly improved environmental stability; the enzyme activity of the mutant is 1051.36 U / mL under the conditions that the temperature is 50 DEG C and the pH value is 7 and is about 2.7 times that of wild chitinase (385.45 U / mL), and the mutant not only has a good killing effect on pests such as lepidoptera pests, thysanoptera pests and coleoptera pests, but also has good bacteriostatic activity on pathogenic bacteria such as phytophthora capsici, pythium aphanidermatum, botrytis cinerea, potato early blight and corn curvularia, and has good application prospects. The fertilizer is used for growth and development of vegetable plants or grain plants, and can promote plant growth and significantly improve the yield and quality of plants.

The invention discloses a single-tube one-step nucleic acid detection method for pythium aphanidermatum and a kit used by the same. The invention relates to the field of vegetable disease detection, and provides a nucleic acid detection reagent for detecting pythium aphanidermatum, which comprises a reagent A and a reagent B, the reagent A is a reagent for performing RPA amplification on target nucleic acid of pythium aphanidermatum; the reagent B is a reagent for carrying out CRISPR / Cas detection on the target nucleic acid; the reagent B contains polyethylene glycol. According to the invention, prefabrication and room temperature storage of a reaction system are realized, on-site preparation is not needed, operation is simple, reaction only needs one-step pipetting, aerosol pollution is avoided, cost is low, and detection of pythium aphanidermatum can be completed without large instruments and equipment and temperature control equipment with relatively high energy consumption. The invention provides an efficient, reliable and economical field detection solution for diagnosing vegetable diseases caused by pythium aphanidermatum.

The application discloses a tobacco biocontrol bacterium, a biocontrol bacterium agent and a propagation method thereof, the bacterium is a strain of Sphingobium sp. PE05 with a preservation number of CCTCC No: M20221104; the strain of Sphingobium sp. PE05 is used for preventing and treating black shank and / or antagonizing Pythium aphanidermatum. The bacterium is separated from healthy tobacco leaves of a cigar tobacco variety of Yunnan Province Puer City Jiangcheng County Yunxue No. 2 and identified as Sphingobium sp., the bacterium is not used for preventing and treating black shank and related bacteria, and the bacterium is not used for antagonizing Pythium aphanidermatum; experiment verification shows that the inhibition rate of the bacterium to Pythium aphanidermatum mycelium growth is as high as 73.5%, the bacterium propagation product has a prevention effect on tobacco black shank of more than 80%, and the bacterium propagation product has the characteristics of low use cost, no residue and safety to human and livestock.

The application discloses a Pseudomonas aeruginosa, which is Pseudomonas aeruginosa EB-Q03-02, and the preservation number is CGMCC No.30779; and the application of the Pseudomonas aeruginosa in preventing and treating Graymold, Pythium aphanidermatum and tobacco blight. The Pseudomonas aeruginosa EB-Q03-02 is separated from the root system of healthy passion fruit, has antagonistic effect on the passion fruit blight, and has obvious antagonistic effect on the passion fruit blight caused by tobacco blight. Further, the Pseudomonas aeruginosa EB-Q03-02 can effectively inhibit the Graymold, Pythium aphanidermatum and tobacco blight, and the inhibition rate is as high as 81% or more.

The application discloses a pythium curvatum ErLAC12 gene and application thereof. The cDNA sequence of the pythium curvatum ErLAC12 gene is shown as SEQ ID NO. 1. The ErLAC12 gene provided by the application can reduce the ROS level of weeds, and has a pathogenic effect on leafflower weeds, and can effectively prevent and control weeds, especially leafflower weeds.

The present application relates to a kind of preparation method of Streptomyces fermentation liquor for biological control, comprising the following steps: Streptomyces spindulus fermentation, centrifugation, take supernatant;The strain name of Streptomyces spindulus is Streptomyces netropsis JN4-2, and the preservation number is CGMCC No.28157.The Streptomyces fermentation liquor can be used for the biological control of red pine pythium, red bean anthracnose, polygenetic small hole shellfish, apple shell monochrome septum bacteria, corn big spot bacteria, tobacco target spot bacteria, rice malignant exanthema bacteria, melon vine cutting bacteria, pepper anthracnose bacteria, larch wilt bacteria, blueberry ulcer disease bacteria, poplar skin disease bacteria, apple rot bacteria, poplar ulcer disease bacteria, eggplant brown mottle bacteria, melon fruit pythium bacteria, tobacco brown spot bacteria, blueberry branch dry disease bacteria, apple fruit rot bacteria, cucumber sclerotinia rot bacteria and other pathogenic bacteria biological control, with the advantages of good biocontrol effect, broad-spectrum bacteriostatic, wide application range and the like.

The application belongs to the technical field of biology, and specifically discloses a Serratia plymuthica R72, which is preserved in the China Center for Type Culture Collection, and the preservation date is February 2, 2026, and the preservation number is CCTCC M 2026311; can be applied to the prevention and treatment of atractylodes rhizome root rot, and the prevention and treatment effect on the main pathogenic bacteria of atractylodes rhizome root rot, i.e., Fusarium oxysporum, is 89.3% in a plate confrontation test; meanwhile, the Serratia plymuthica R72 provided by the application has strong antagonistic effects on the pathogenic bacteria of rice false smut, sclerotinia sclerotiorum, sclerotinia sclerotiorum, tomato gray mold, melon fruit rot, brassica nigra, rice blast, rice sesame leaf spot, and tomato wilt, and the inhibition rate is more than 88%.