Plant nutrition sterilization suspension agent prepared based on natural substances
By preparing a plant nutrient bactericidal suspension based on natural substances, the pollution problem of chemical pesticides has been solved, achieving the dual effects of efficient bactericidal and nutritional benefits, and improving the plant's disease resistance and yield.
Patent Information
- Application Number
- CN202510971850.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-15
- Publication Date
- 2025-11-28
AI Technical Summary
Most existing insecticides and fungicides are chemical pesticides, which have low efficacy, long residual toxicity, environmental pollution, and pests are prone to developing resistance. At the same time, there is a lack of fungicides that provide nutrients, which cannot effectively improve the plant's disease resistance.
A plant-based nutrient bactericidal suspension was prepared using natural substances such as mixed plant extracts, wood ash leachate, mangosteen shell extract, hibiscus, animal excrement, and lotus pond silt. The suspension was then processed through ultrasonic-assisted extraction and microbial fermentation to form a suspension with both bactericidal and nutrient effects.
It improves the prevention and control of viruses, increases plant nutrition, increases yield, and causes less soil pollution. It has a highly effective bactericidal effect and safety, and the pathogen inhibition effect reaches more than 95%.
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Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of plant nutrition fungicide, more particularly, the present application relates to a plant nutrition fungicide suspension agent prepared based on natural substances. BACKGROUND
[0002] The existing technology of insecticide and fungicide is mostly chemical pesticide, which has low efficacy, long residual toxicity, and seriously affects the growth environment of crops and the health of human and livestock. Moreover, due to the large use of chemical pesticide, the air, water, soil and food are polluted, and the accumulation of toxic substances in livestock and human body causes poisoning. According to relevant professional literature, the utilization rate of pesticide in agricultural production is generally only 10%, and about 90% of the residual amount of pesticide remains in the environment, causing pollution to the environment. A large amount of pesticide volatilizes into the air, flows into the water, and settles and accumulates in the soil, polluting agricultural and livestock products, and is transferred to the human body through the enrichment of the food chain, causing harm to the human body.
[0003] A biological pesticide and its preparation method are disclosed in Chinese patent library (200510056647.7), which is a pollution-free pesticide prepared by compounding organic substances extracted from Tripterygium plant bodies with tea saporin. The present application is characterized in that the Tripterygium plant bodies are processed by cutting, crushing, sieving, sterile extraction, filtration and concentration, and the biological pesticide in the form of emulsion, water emulsion, microemulsion, etc. can be prepared by compounding with tea saporin. The main components of the biological pesticide of the present application are Tripterygium extract <90%, tea saporin 0.1%-30%, which has the advantages of simple process, low cost, broad spectrum, synergistic effect, strong adsorption, low toxicity, low residue, etc. At the same time, pests are not easy to develop resistance to it, which meets the development direction of biological pesticide at present, and has a wide application prospect in the prevention and control of plant pests such as vegetables, melons and fruits, fruit trees and various economic tree species.
[0004] However, the above-mentioned biological pesticide is often effective only against one or two kinds of pests, and cannot provide nutrition. When a plant is diseased, the plant's ability to resist disease is weak due to unbalanced nutrition in the body, and there is a lack of fungicide nutrition that combines disease prevention, treatment and physiological nutrition functions. SUMMARY
[0005] In order to overcome the above-mentioned defects of the prior art, the embodiments of the present application provide a plant nutrition fungicide suspension agent prepared based on natural substances, and the problem to be solved by the present application is how to improve the prevention and control effect of the plant fungicide on viruses, increase the nutrition of plants, and improve the yield.
[0006] To achieve the above objectives, the present invention provides the following technical solution: a plant nutrient bactericidal suspension prepared based on natural substances, wherein the plant nutrient bactericidal suspension comprises the following raw materials by weight percentage: 25-40% mixed plant extract, 5-15% wood ash leachate, 5-10% mangosteen peel extract, 2-5% trace elements, 1-3% citric acid, 2-6% hibiscus, 2-5% animal manure, 1-5% lotus pond silt, and the remainder being deionized water.
[0007] In a preferred embodiment, the plant nutrient bactericidal suspension comprises the following raw materials by weight percentage: 30-35% mixed plant extract, 8-12% wood ash leachate, 7-9% mangosteen peel extract, 3-4% trace elements, 1.5-2.5% citric acid, 3-5% hibiscus, 3-4% animal manure, 2-4% lotus pond silt, and the remainder being deionized water.
[0008] In a preferred embodiment, the plant nutrient bactericidal suspension comprises the following raw materials by weight percentage: 32% mixed plant extract, 10% wood ash leachate, 8% mangosteen shell extract, 3.5% trace elements, 2% citric acid, 4% hibiscus, 3.5% animal manure, 3% lotus pond silt, and the remainder being deionized water.
[0009] In a preferred embodiment, the plant extract mixture is made from the following raw materials in parts by weight: 5-10 parts mimosa, 3-8 parts bitter vine, 2-6 parts garlic, 2-6 parts peppermint, 5-10 parts marigold, 2-6 parts clove, and 1-5 parts tea leaves.
[0010] In a preferred embodiment, the plant extract mixture is made from the following raw materials in parts by weight: 8 parts mimosa, 5 parts bitter vine, 4 parts garlic, 4 parts peppermint, 8 parts marigold, 4 parts clove, and 3 parts tea leaves.
[0011] In a preferred embodiment, the trace elements are metal salts or metal oxides of potassium, zinc, magnesium, and calcium.
[0012] In a preferred embodiment, the method for preparing the plant nutrient bactericidal suspension is as follows: Step 1: Weigh out the raw materials of mimosa, bitter vine, garlic, mint, marigold, clove and tea according to the ratio of the plant mixed extract. Mix them and put them into an ultra-micro pulverizer for pulverization and sieving. Add water to the sieved raw materials and extract twice under the assistance of ultrasound. Combine the two extracts to obtain the plant mixed extract for later use. Step 2: Rinse, dry, and crush the selected mangosteen shells. Add 75% ethanol solution to the crushed mangosteen shells, heat to 60-70℃, and extract for 30-60 minutes with ultrasonic assistance. Purify the extract using macroporous adsorption resin to obtain mangosteen shell extract for later use. Step 3: Add microbial fermentation agent to the weighed hibiscus, animal manure and lotus pond silt, ferment at 50-60℃ for 16-24 hours, and then perform solid-liquid separation to obtain a premixed liquid; Step 4: Weigh the plant mixed extract obtained in Step 1, the mangosteen fruit shell extract obtained in Step 2, and the premixed liquid obtained in Step 3, stir and mix them, then add the wood ash leachate, trace elements, citric acid and water, mix evenly to obtain the plant nutrient bactericidal suspension.
[0013] In a preferred embodiment, in step one, the material is pulverized by an ultra-fine pulverizer and then passed through a 200-230 mesh sieve. In the two extractions in step one, 8 times the amount of water is added during the first extraction, and the mixture is decocted at 70-90℃ for 2-3 hours. During the second extraction, 6 times the amount of water is added, and the mixture is decocted at 90-100℃ for 1.5-2 hours.
[0014] In a preferred embodiment, when ultrasonic assistance is used in steps one and two, a 40kHz, 200W ultrasonic wave is used, and the macroporous adsorption resin is a D101 adsorption resin with an adsorption capacity of 25-30mg / g. After adsorption is completed, elution is performed using 70% ethanol.
[0015] In a preferred embodiment, the microbial fermentation agent in step three is a mixture of Bacillus stearothermophilus, Bacillus amyloliquefaciens, and thermophilic lactic acid bacteria, and the mass ratio of Bacillus stearothermophilus, Bacillus amyloliquefaciens, and thermophilic lactic acid bacteria is 1:(0.5-0.8):(1.2-1.8).
[0016] The technical effects and advantages of this invention are as follows: 1. The plant nutrient bactericidal suspension prepared based on natural substances produced by this invention uses natural substances such as mixed plant extracts, wood ash leachate, mangosteen peel extract, hibiscus, animal manure, and lotus pond silt as raw materials. When used for plant sterilization, it causes less soil pollution and has dual effects of sterilization and nutrition. The mixed plant extracts include ingredients such as mimosa, bitter vine, garlic, mint, marigold, clove, and tea. It is effective against wheat powdery mildew, cotton damping-off, cucumber angular leaf spot, tobacco mosaic virus, pyrophyllosis of melons and fruits, and cucumber anthracnose. The fungus has a high inhibitory effect, with an inhibition rate of about 95% against tomato gray mold and leaf mold. It is non-toxic and has high safety. The mangosteen fruit shell extract contains a lot of flavonoids and polyphenols, which can not only improve the antibacterial effect of plants, but also provide nutritional benefits to plants. The wood ash leachate and trace elements can provide nutrients to plants. The wood ash leachate contains a lot of potassium, which can improve the nutritional components of plants, as well as enhance their resistance to lodging, disease, and cold. It also has a control effect on powdery mildew, gray mold, and downy mildew.
[0017] 2. The hibiscus, animal manure and pond silt added in this invention, after being treated with a microbial fermentation agent, can ferment the organic matter and bacteria in the animal manure and pond silt, which is convenient for plant absorption. The hibiscus can provide plants with more polysaccharides and trace elements such as selenium and zinc, and produce humic acid, which can improve the soil. Detailed Implementation
[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the embodiments thereof. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention. Example
[0019] This invention provides a plant-based nutrient bactericidal suspension prepared from natural substances. The plant-based nutrient bactericidal suspension comprises the following raw materials by weight percentage: 25% mixed plant extract, 5% wood ash leachate, 5% mangosteen shell extract, 2% trace elements, 1% citric acid, 2% hibiscus, 2% animal manure, 1% lotus pond silt, and the remainder is deionized water.
[0020] In a preferred embodiment, the plant extract mixture is made from the following raw materials in parts by weight: 5 parts mimosa, 3 parts bitter vine, 2 parts garlic, 2 parts peppermint, 5 parts marigold, 2 parts clove, and 1 part tea leaves.
[0021] In a preferred embodiment, the trace elements are metal salts or metal oxides of potassium, zinc, magnesium, and calcium.
[0022] In a preferred embodiment, the method for preparing the plant nutrient bactericidal suspension is as follows: Step 1: Weigh out the raw materials of mimosa, bitter vine, garlic, mint, marigold, clove and tea according to the ratio of the plant mixed extract. Mix them and put them into an ultra-micro pulverizer for pulverization and sieving. Add water to the sieved raw materials and extract twice under the assistance of ultrasound. Combine the two extracts to obtain the plant mixed extract for later use. Step 2: Rinse, dry, and crush the selected mangosteen shells. Add 75% ethanol solution to the crushed mangosteen shells, heat to 65℃, and extract for 45 minutes under ultrasonic assistance. Purify the extract using macroporous adsorption resin to obtain mangosteen shell extract for later use. Step 3: Add microbial fermentation agent to the weighed hibiscus, animal manure and lotus pond silt, ferment at 55℃ for 20 hours, and then perform solid-liquid separation to obtain a premixed liquid; Step 4: Weigh the plant mixed extract obtained in Step 1, the mangosteen fruit shell extract obtained in Step 2, and the premixed liquid obtained in Step 3, stir and mix them, then add the wood ash leachate, trace elements, citric acid and water, mix evenly to obtain the plant nutrient bactericidal suspension.
[0023] In a preferred embodiment, in step one, the material is pulverized by an ultra-fine pulverizer and then passed through a 220-mesh sieve. In the two extractions in step one, 8 times the amount of water is added during the first extraction and the mixture is decocted at 80°C for 2.5 hours. During the second extraction, 6 times the amount of water is added and the mixture is decocted at 95°C for 2 hours.
[0024] In a preferred embodiment, when ultrasonic assistance is used in steps one and two, a 40kHz, 200W ultrasonic wave is used, and the macroporous adsorption resin is a D101 adsorption resin with an adsorption capacity of 28mg / g. After adsorption is completed, elution is performed using 70% ethanol.
[0025] In a preferred embodiment, the microbial fermentation agent in step three is a mixture of Bacillus stearothermophilus, Bacillus amyloliquefaciens, and thermophilic lactic acid bacteria, and the mass ratio of Bacillus stearothermophilus, Bacillus amyloliquefaciens, and thermophilic lactic acid bacteria is 1:0.6:1.5. Example
[0026] Unlike Example 1, the plant nutrient bactericidal suspension contains the following raw materials by weight percentage: 32% mixed plant extract, 10% wood ash leachate, 8% mangosteen shell extract, 3.5% trace elements, 2% citric acid, 4% hibiscus, 3.5% animal manure, 3% lotus pond silt, and the remainder is deionized water. Example
[0027] Unlike Examples 1-2, the plant nutrient bactericidal suspension agent comprises the following raw materials by weight percentage: 40% mixed plant extract, 15% wood ash leachate, 10% mangosteen peel extract, 5% trace elements, 3% citric acid, 6% hibiscus, 5% animal manure, 5% lotus pond silt, and the remainder is deionized water. Example
[0028] Unlike Example 1, the plant extract mixture is made from the following ingredients in parts by weight: 8 parts mimosa, 5 parts bitter vine, 4 parts garlic, 4 parts peppermint, 8 parts marigold, 4 parts clove, and 3 parts tea leaves. Example
[0029] Unlike Example 1, the plant extract mixture is made from the following ingredients in parts by weight: 10 parts mimosa, 8 parts bitter vine, 6 parts garlic, 6 parts peppermint, 10 parts marigold, 6 parts clove, and 5 parts tea leaves.
[0030] Comparative Example 1: This invention provides a plant nutrient bactericidal suspension prepared based on natural substances. The plant nutrient bactericidal suspension prepared based on natural substances comprises the following raw materials in weight percentage: 25% plant extract, 5% wood ash leachate, 5% mangosteen shell extract, 2% trace elements, 1% citric acid, 2% hibiscus, 2% animal manure, 1% lotus pond silt, and the remainder is deionized water.
[0031] In a preferred embodiment, the plant extract is a mimosa extract.
[0032] In a preferred embodiment, the trace elements are metal salts or metal oxides of potassium, zinc, magnesium, and calcium.
[0033] In a preferred embodiment, the method for preparing the plant nutrient bactericidal suspension is as follows: Step 1: Weigh out the mimosa plant and put it into an ultra-fine pulverizer to pulverize and sieve. Add water to the sieved raw material and extract it twice under the assistance of ultrasound. Combine the two extracts to obtain the plant extract for later use. Step 2: Rinse, dry, and crush the selected mangosteen shells. Add 75% ethanol solution to the crushed mangosteen shells, heat to 65℃, and extract for 45 minutes under ultrasonic assistance. Purify the extract using macroporous adsorption resin to obtain mangosteen shell extract for later use. Step 3: Add microbial fermentation agent to the weighed hibiscus, animal manure and lotus pond silt, ferment at 55℃ for 20 hours, and then perform solid-liquid separation to obtain a premixed liquid; Step 4: Weigh the plant mixed extract obtained in Step 1, the mangosteen fruit shell extract obtained in Step 2, and the premixed liquid obtained in Step 3, stir and mix them, then add the wood ash leachate, trace elements, citric acid and water, mix evenly to obtain the plant nutrient bactericidal suspension.
[0034] In a preferred embodiment, in step one, the material is pulverized by an ultra-fine pulverizer and then passed through a 220-mesh sieve. In the two extractions in step one, 8 times the amount of water is added during the first extraction and the mixture is decocted at 80°C for 2.5 hours. During the second extraction, 6 times the amount of water is added and the mixture is decocted at 95°C for 2 hours.
[0035] In a preferred embodiment, when ultrasonic assistance is used in steps one and two, a 40kHz, 200W ultrasonic wave is used, and the macroporous adsorption resin is a D101 adsorption resin with an adsorption capacity of 28mg / g. After adsorption is completed, elution is performed using 70% ethanol.
[0036] In a preferred embodiment, the microbial fermentation agent in step three is a mixture of Bacillus stearothermophilus, Bacillus amyloliquefaciens, and thermophilic lactic acid bacteria, and the mass ratio of Bacillus stearothermophilus, Bacillus amyloliquefaciens, and thermophilic lactic acid bacteria is 1:0.6:1.5.
[0037] Comparative Example 2: This invention provides a plant nutrient bactericidal suspension prepared based on natural substances. The plant nutrient bactericidal suspension prepared based on natural substances comprises the following raw materials in weight percentage: 25% mixed plant extract, 5% wood ash leachate, 5% mangosteen fruit shell extract, 2% trace elements, 1% citric acid, and the remainder being deionized water.
[0038] In a preferred embodiment, the plant extract mixture is made from the following raw materials in parts by weight: 5 parts mimosa, 3 parts bitter vine, 2 parts garlic, 2 parts peppermint, 5 parts marigold, 2 parts clove, and 1 part tea leaves.
[0039] In a preferred embodiment, the trace elements are metal salts or metal oxides of potassium, zinc, magnesium, and calcium.
[0040] In a preferred embodiment, the method for preparing the plant nutrient bactericidal suspension is as follows: Step 1: Weigh out the raw materials of mimosa, bitter vine, garlic, mint, marigold, clove and tea according to the ratio of the plant mixed extract. Mix them and put them into an ultra-micro pulverizer for pulverization and sieving. Add water to the sieved raw materials and extract twice under the assistance of ultrasound. Combine the two extracts to obtain the plant mixed extract for later use. Step 2: Rinse, dry, and crush the selected mangosteen shells. Add 75% ethanol solution to the crushed mangosteen shells, heat to 65℃, and extract for 45 minutes under ultrasonic assistance. Purify the extract using macroporous adsorption resin to obtain mangosteen shell extract for later use. Step 3: Weigh the plant mixed extract obtained in Step 1 and the mangosteen fruit shell extract obtained in Step 2, stir and mix them, then add the wood ash leachate, trace elements, citric acid and water, mix evenly to obtain a plant nutrient bactericidal suspension.
[0041] In a preferred embodiment, in step one, the material is pulverized by an ultra-fine pulverizer and then passed through a 220-mesh sieve. In the two extractions in step one, 8 times the amount of water is added during the first extraction and the mixture is decocted at 80°C for 2.5 hours. During the second extraction, 6 times the amount of water is added and the mixture is decocted at 95°C for 2 hours.
[0042] In a preferred embodiment, when ultrasonic assistance is used in steps one and two, a 40kHz, 200W ultrasonic wave is used, and the macroporous adsorption resin is a D101 adsorption resin with an adsorption capacity of 28mg / g. After adsorption is completed, elution is performed using 70% ethanol.
[0043] The plant nutrient bactericidal suspensions obtained in Examples 1-5 were used as experimental groups 1, 2, 3, 4 and 5, respectively. The plant nutrient bactericidal suspensions obtained in Comparative Examples 1-2 were used as control groups 1 and 2, respectively. The selected plant nutrient bactericidal suspensions were applied to tomato plant culture to test the antibacterial and nutritional effects of the plant nutrient bactericidal suspensions on tomatoes.
[0044] Tomatoes were grown using conventional techniques and randomly divided into 8 plots, each with an area of 80 square meters. 2 An investigation was conducted. Water spraying served as a blank control group. The fungicides prepared in Examples 1-5 and Comparative Examples 1-2 were diluted with water to a concentration of 3 g / L and sprayed onto the leaves until a thin water film appeared. The control rates against tomato gray mold and tomato anthracnose were then measured 10 days after application, and the yield per acre for each plot was also measured.
[0045] The measurement results are shown in Table 1:
[0046] Table 1 As can be seen from Table 1, the plant nutrient bactericidal suspension produced in this embodiment has a good effect on preventing plant viruses and a high effect on adding nutrients to plants, resulting in increased yield. In contrast, Comparative Example 1, which uses a single plant extract, has a significantly reduced virus prevention effect and a slightly reduced yield. Comparative Example 2, which does not contain raw materials such as hibiscus, animal manure, and pond silt, has a significantly reduced yield. Therefore, this invention uses natural substances such as mixed plant extracts, wood ash leachate, mangosteen shell extract, hibiscus, animal manure, and lotus pond silt as raw materials. When used for plant sterilization, it causes less soil pollution and has dual effects of sterilization and nutrition. The mixed plant extract contains ingredients such as mimosa, bitter vine, garlic, mint, marigold, clove, and tea, which have high inhibitory effects on wheat powdery mildew, cotton damping-off, cucumber angular leaf spot, tobacco mosaic virus, pyrophyllosis of melons and fruits, and cucumber anthracnose. It also has an inhibition rate of approximately 95% against tomato gray mold and leaf mold, is non-toxic, and has high safety. The mangosteen shell extract contains a relatively high amount of flavonoids and polyphenols. This invention not only enhances the antibacterial effect of plants but also provides them with nutrients. The wood ash leachate and trace elements provide nutrients to the plants. The wood ash leachate contains a relatively high amount of potassium, which not only improves the nutritional content of the plants but also enhances their resistance to lodging, diseases, and cold. It also has a control effect on powdery mildew, gray mold, and downy mildew. The addition of hibiscus, animal manure, and pond silt to this invention, after being treated with a microbial fermentation agent, can ferment the organic matter and bacteria in the animal manure and pond silt, facilitating plant absorption. Hibiscus provides the plants with a relatively high amount of polysaccharides and trace elements such as selenium and zinc, and produces humic acid, which can improve the soil.
[0047] In conclusion, the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A plant-based nutrient bactericidal suspension prepared from natural substances, characterized in that: The plant nutrient bactericidal suspension agent comprises the following raw materials by weight percentage: 25-40% mixed plant extract, 5-15% wood ash leachate, 5-10% mangosteen shell extract, 2-5% trace elements, 1-3% citric acid, 2-6% hibiscus, 2-5% animal manure, 1-5% lotus pond silt, and the remainder is deionized water.
2. The plant nutrient bactericidal suspension agent prepared based on natural substances according to claim 1, characterized in that: The plant nutrient bactericidal suspension agent comprises the following raw materials by weight percentage: 30-35% mixed plant extract, 8-12% wood ash leachate, 7-9% mangosteen shell extract, 3-4% trace elements, 1.5-2.5% citric acid, 3-5% hibiscus, 3-4% animal manure, 2-4% lotus pond silt, and the remainder is deionized water.
3. The plant nutrient bactericidal suspension agent prepared based on natural substances according to claim 1, characterized in that: The plant nutrient bactericidal suspension contains the following raw materials by weight percentage: 32% mixed plant extract, 10% wood ash leachate, 8% mangosteen shell extract, 3.5% trace elements, 2% citric acid, 4% hibiscus, 3.5% animal manure, 3% lotus pond silt, and the remainder is deionized water.
4. The plant nutrient bactericidal suspension agent prepared based on natural substances according to claim 1, characterized in that: The plant extract is made from the following raw materials in parts by weight: 5-10 parts mimosa, 3-8 parts bitter vine, 2-6 parts garlic, 2-6 parts peppermint, 5-10 parts marigold, 2-6 parts clove, and 1-5 parts tea leaves.
5. A plant nutrient bactericidal suspension agent prepared based on natural substances according to claim 4, characterized in that: The plant extract is made from the following ingredients in parts by weight: 8 parts mimosa, 5 parts bitter vine, 4 parts garlic, 4 parts peppermint, 8 parts marigold, 4 parts clove, and 3 parts tea leaves.
6. The plant nutrient bactericidal suspension agent prepared based on natural substances according to claim 1, characterized in that: The trace elements are metal salts or metal oxides of potassium, zinc, magnesium, and calcium.
7. The plant nutrient bactericidal suspension agent prepared based on natural substances according to claim 1, characterized in that: The preparation method of the plant nutrient bactericidal suspension is as follows: Step 1: Weigh out the raw materials of mimosa, bitter vine, garlic, mint, marigold, clove and tea according to the ratio of the plant mixed extract. Mix them and put them into an ultra-micro pulverizer for pulverization and sieving. Add water to the sieved raw materials and extract twice under the assistance of ultrasound. Combine the two extracts to obtain the plant mixed extract for later use. Step 2: Rinse, dry, and crush the selected mangosteen shells. Add 75% ethanol solution to the crushed mangosteen shells, heat to 60-70℃, and extract for 30-60 minutes with ultrasonic assistance. Purify the extract using macroporous adsorption resin to obtain mangosteen shell extract for later use. Step 3: Add microbial fermentation agent to the weighed hibiscus, animal manure and lotus pond silt, ferment at 50-60℃ for 16-24 hours, and then perform solid-liquid separation to obtain a premixed liquid; Step 4: Weigh the plant mixed extract obtained in Step 1, the mangosteen fruit shell extract obtained in Step 2, and the premixed liquid obtained in Step 3, stir and mix them, then add the wood ash leachate, trace elements, citric acid and water, mix evenly to obtain the plant nutrient bactericidal suspension.
8. A plant nutrient bactericidal suspension agent prepared based on natural substances according to claim 7, characterized in that: In step one, the material is pulverized by an ultra-fine pulverizer and then passed through a 200-230 mesh sieve. In the two extractions in step one, 8 times the amount of water is added for the first extraction and the mixture is decocted at 70-90℃ for 2-3 hours. For the second extraction, 6 times the amount of water is added and the mixture is decocted at 90-100℃ for 1.5-2 hours.
9. A plant nutrient bactericidal suspension agent prepared based on natural substances according to claim 7, characterized in that: In steps one and two, ultrasonic waves of 40kHz and 200W are used for ultrasonic assistance. The macroporous adsorption resin used is D101 adsorption resin with an adsorption capacity of 25-30mg / g. After adsorption is completed, elution is performed using 70% ethanol.
10. A plant nutrient bactericidal suspension agent prepared based on natural substances according to claim 7, characterized in that: In step three, the microbial fermentation agent is a mixture of Bacillus stearothermophilus, Bacillus amyloliquefaciens, and thermophilic lactic acid bacteria, and the mass ratio of Bacillus stearothermophilus, Bacillus amyloliquefaciens, and thermophilic lactic acid bacteria is 1:(0.5-0.8):(1.2-1.8).
Citation Information
Patent Citations
Biological pesticide and preparation method thereof
CN1666619A
