17 results about "Aspergillus ochraceus" patented technology

The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus capable of inhibiting growth of monascus and application of the plant lactobacillus. The preservation number of the plant lactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.34851. According to the present invention, the plant lactobacillus CCNH295 has strong bacterial inhibition ability on common soybean meal contaminating molds, such as aspergillus flavus, aspergillus ochraceus, fusarium moniliforme and penicillium; the plant lactobacillus CCNH295 has excellent bacteriostatic ability to monascus, and when the plant lactobacillus CCNH295 is used for fermentation production of soybean meal, growth of monascus can be remarkably inhibited, so that mildewing of the fermented soybean meal is effectively inhibited, and the prepared fermented soybean meal does not mildew after being placed at 37 DEG C for 6 months; besides, the fermented soybean meal produced by solid state fermentation of the plant lactobacillus CCNH295 is used as a feed raw material, so that the stability of the feed can be improved, and the palatability of the feed can be improved.

The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.

The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method of fermented pinellia tuber, which comprises the following specific steps: S1, crushing rhizoma pinelliae preparata and medicated leaven into fine powder; s2, preparing ginger juice; s3, dissolving alum in a proper amount of water, adding the ginger juice, uniformly mixing, and uniformly mixing with the rhizoma pinelliae preparata, the medicated leaven, the flour and the raw bran which are crushed into fine powder; s4, knocking out an ochratoxin synthetic gene (otaA) from the aspergillus oryzae CICC 40186 through CRISPR-Cas9, and transferring the ochratoxin synthetic gene (otaA) into a lactic dehydrogenase gene (ldhL) of lactobacillus plantarum; s5, preparing a compound bacterium suspension; s6, placing the inoculated material in a solid state fermentation reactor; s7, vacuum freeze drying and variable-temperature drying are combined; the consistency of components of different batches of fermented pinellia can be guaranteed, the risk of infectious microbe contamination can be avoided, ochratoxin can be completely eradicated, the fermentation period can be greatly shortened, the overall production efficiency can be improved, the content of the key active component gamma-aminobutyric acid can be greatly improved, and the stability between batches is good.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The application belongs to the technical field of genetic engineering and enzyme engineering, and discloses a C11 alpha-hydroxylase mutant D118V with improved catalytic activity, wherein the amino acid sequence is shown as SEQ ID NO. 4, and the corresponding nucleotide sequence is shown as SEQ ID NO. 5. The C11 alpha-hydroxylase from Aspergillus ochraceus is subjected to site-directed saturation mutation, and the C11 alpha-hydroxylase mutant D118V for improving the yield of 11 alpha, 17 alpha-dihydroxy progesterone is obtained by using a Saccharomyces cerevisiae expression platform. When the concentration of the substrate 17 alpha-hydroxy progesterone is 2 g / L, the concentration of 11 alpha, 17 alpha-dihydroxy progesterone after 36 h of conversion is 1.19 g / L, and the production intensity is 758.15 mg / (L·d), which is increased by 16.67% and 172.77% compared with the wild-type CYP68J5, thereby providing an application case and basic data support for the modification of steroid C11 alpha-hydroxylase.

The application discloses a method for detecting ochratoxin A in milk based on a double-identification electrochemical sensor, and compared with other OTA detection methods, the sensor has a wider linear range (0-300nM) and a lower LOD (0.09nM) under optimal conditions; the sensor has high repeatability, stability and specificity, and the sensor has good feasibility in detection of different types of milk samples, and a good recovery rate of 95.4%-105.6% is obtained.

The present application relates to the technical field of harmful substance detection, in particular to a fluorescent probe for detecting ochratoxin A and a preparation method and application thereof.The fluorescent probe for detecting ochratoxin A is a complex of a fluorescent dye DOCE and serum albumin combined by hydrogen bonds; wherein the chemical structural formula of the fluorescent dye DOCE is as shown.The fluorescent probe prepared by the present application is used for rapid quantitative or qualitative detection of ochratoxin A (OTA), and the detection method has an ultrafast response speed (5 seconds), high sensitivity (detection limit is 0.39 ppb), and high selectivity, and has been successfully applied to on-site rapid detection of real food samples such as flour, white wine and red wine.

The application provides an anti-disease promoting Aspergillus ochraceus AF-P9 and an application thereof, and belongs to the technical field of microorganisms.The application provides the Aspergillus ochraceus AF-P9, which has salt tolerance and alkali tolerance, has the ability of producing iron carrier and secreting chitinase, has a high efficient inhibiting effect on multiple pathogenic bacteria such as Fusarium oxysporum, Fusarium acuminatum, Fusarium decemcellulare, Pythium aphanidermatum, Pseudomonas syringae pv., Sclerotinia sclerotiorum and Phytophthora, and can be applied to the prevention and treatment of rice damping-off.Further, metabolites of the Aspergillus ochraceus AF-P9 are analyzed, and it is found that the Aspergillus ochraceus AF-P9 can produce tricyclic cyclohexanone compounds, cyclohexene compounds with hydroxyl, phenanthrene ketone compounds with hydroxyl, benzene ring compounds with two long chain esters and cyclohexene compounds with four annular structures.The Aspergillus ochraceus AF-P9 provided by the application provides a strain resource for the prevention and treatment of disease organisms.

This invention discloses a method for controlling the biotransformation of canlione by monitoring the expression level of hydroxylase gene. The steps are as follows: using *Aspergillus ochraceus* as the inoculum and canlione as the substrate, the expression level of the hydroxylase gene is monitored using real-time quantitative PCR during cell culture and transformation to determine the mid-logarithmic growth phase of the cell culture; canlione is then biotransformed again using *Aspergillus ochraceus* as the inoculum and canlione as the substrate, with an exogenous inducer added during the mid-logarithmic growth phase of the cell culture to improve the transformation rate. This method uses the hydroxylase gene expression level, a molecular biological indicator, as a key process parameter to guide the decision-making of substrate addition timing and the optimization of transformation conditions. It enables precise control of process optimization driven by gene expression data, achieving a significant increase in transformation rate and a significant reduction in transformation cycle, showing promising application prospects.

The invention discloses a composite nanomaterial for inhibiting and degrading fungaltoxin as well as a preparation method and application thereof, belongs to the technical field of biochemical engineering, and solves the technical problem that a degradable material with broad-spectrum high efficiency, environmental stability and industrial feasibility is lacked in the prior art. The preparation method of the composite nano material comprises the following steps: dissolving a polyphenol component and a metal salt component in a Tris-HCl buffer solution according to a molar ratio of 1: (1-3), controlling the pH value of the solution to be 4-6, stirring and reacting at 20-60 DEG C for 1-3 hours to obtain a self-assembly solution, dropwise adding an activated carbon quantum dot solution into the self-assembly solution, stirring in a dark place for 12-18 hours to obtain a reaction solution, and centrifuging the reaction solution to obtain a precipitate; washing the precipitate with water for 3-5 times, and drying to obtain the composite nano material. The composite nano material is used for inhibiting growth of aspergillus flavus, aspergillus parasiticus or aspergillus ochraceus and degrading fungaltoxin generated by the aspergillus flavus, the aspergillus parasiticus or the aspergillus ochraceus, and the antibacterial efficiency reaches 95% or above.

The invention discloses an Aspergillus ochraceus Slt L1 strain and application thereof. The Aspergillus ochraceus Slt L1 strain has the morphological characteristics that after the Aspergillus ochraceus Slt L1 strain is cultured on a PDA culture medium for 7 days, typical Aspergillus ochraceus colony characteristics are formed, hyphae are separated and branched, conidiophore is upright, and conidia are spherical to nearly spherical. The prodenia litura strain has obvious pathogenicity to prodenia litura eggs, can lead to 100% of egg death rate, and has wide metabolic adaptability and environmental tolerance. Through the determination of a Biolog phenotype chip system, the bacterium can efficiently utilize a carbon source, a nitrogen source, a phosphorus source, a sulfur source and a plurality of biosynthetic pathways, and can keep the growth activity under the extreme conditions of osmotic pressure and pH. The microbial inoculum can be used for biological control of prodenia litura, and has the characteristics of greenness, high efficiency, environmental friendliness and the like.

The invention discloses lactobacillus mucilaginosus for inhibiting the growth of aspergillus ochraceus and application of the lactobacillus mucilaginosus. The lactobacillus fermentum is named as the lactobacillus fermentum A119, and the strain is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CGMCC NO. The preservation number of the strain is CCTCC NO: M 2025001. According to the present invention, the bacterial strain can provide a strong antibacterial effect on pathogenic microorganisms such as Aspergillus ochraceus BNCC144159, Penicillium expansum BNCC117714, Staphylococcus aureus ATCC25923, Listeria monocytogenes CMCC21633, Escherichia coli CICC10389, Salmonella WX29, Pseudomonas aeruginosa ATCC27853 and the like, and can be used for food fresh keeping, and the bacterial strain can be used for food fresh keeping,

The invention provides a multi-stage purification method for reducing ochratoxin A in a licorice extract, and belongs to the technical field of licorice extract purification, the multi-stage purification method for reducing ochratoxin A in a licorice extract comprises the following steps: adding a licorice raw material and an extraction solvent into an extraction tank, starting a first motor, and mixing for 30-60 minutes to form a licorice extract; an extracting solution flows into a first screening sleeve through a conveying pipe at the bottom end of the extracting tank, stirring mixing and auxiliary flowing are conducted on the extracting solution, a second filter screen in the first screening sleeve is used for conducting preliminary screening on the extracting solution, and large-particle impurities are removed; the extracting solution obtained after preliminary screening flows into a second screening sleeve through a conveying opening, a second filter screen further conducts fine screening on the extracting solution, and smaller particle impurities are removed; the extracting solution subjected to two-stage screening flows into the column passing leaching assembly in the supporting sleeve, and the extracting solution uniformly flows through the interior of the purification column under the effect of gravity and turbulent flow generated by rotation of the purification column to remove the ochratoxin A.

The invention relates to the technical field of microorganisms, and discloses a novel sphingobacterium sp. And application thereof, the novel sphingobacterium sp. Is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 67028. The invention also provides a biological detoxication agent containing the preserved strain and a preparation method of the biological detoxication agent. In addition, the invention also provides an application of the strain or the biological detoxication agent in degrading ochratoxin A, and an application of the strain or the biological detoxication agent in inhibiting aspergillus ochraceus growth and toxin production.

The invention discloses a double-enzyme sensing system for detecting ochratoxin A and application of the double-enzyme sensing system. The invention provides a method for detecting OTA in a to-be-detected sample, which comprises the following steps: A1) carrying out hydrolysis reaction on the to-be-detected sample by using amide hydrolase to obtain a first-step reaction solution; a2) adding phenylalanine dehydrogenase, coenzyme NAD < + > and Glycine into the first-step reaction solution, and reacting again to obtain a second-step reaction solution; adding a color developing agent into the second-step reaction solution, and carrying out color developing reaction to obtain a reaction product; detecting OTA in the sample to be detected according to the reaction product; according to the invention, a novel OTA degrading enzyme ThADH of an OTA sensing element and a product L-phenylalanine sensing element phenylalanine dehydrogenase are combined, and efficient, visual and quantitative detection of OTA is realized by using a color reaction in a product catalysis process.

This invention discloses a fermenting *Lactobacillus mucilaginosus* strain that inhibits the growth of *Aspergillus ochraceus* and its applications. The fermenting *Lactobacillus mucilaginosus* is named *Lactobacillus mucilaginosus* (…). Limosilactobacillus leaven The strain A119 is deposited at the China Center for Type Culture Collection (CCTCC); accession number CCTCC NO: M 2025001. This strain is resistant to Aspergillus ochraceus. Aspergillus ochraceus BNCC144159, Penicillium Penicillium expansum BNCC117714 exhibits strong antibacterial effects against pathogenic microorganisms such as Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Salmonella, and Pseudomonas aeruginosa, and can be used for food preservation.

The invention provides a composite material with both toxin-producing fungus resisting activity and fungaltoxin degrading effect. According to the invention, montmorillonite is taken as a carrier, and the advantages of bacillus, trichoderma reesei extracellular enzyme, cinnamyl aldehyde and montmorillonite are combined and compounded into a composite material with both toxin-producing fungus activity resistance and mycotoxin degradation effect, so that the composite material has both toxin-producing fungus inhibition and mycotoxin degradation effects; the compound can be applied to feed and grain products to inhibit mildew and reduce mycotoxin pollution. When the composite material is added into the corn flour polluted by toxin-producing bacteria and mycotoxin, the composite material can significantly inhibit the growth of toxin-producing fungi (fusarium graminearum, fusarium equiseti, aspergillus flavus and aspergillus ochraceus) in the corn flour, and the AFB1 pollution inhibition rate and the ZEN pollution inhibition rate are 98.89% and 93.54% respectively.