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4 results about "Aspergillus ochraceus" patented technology
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Aspergillus ochraceus is a mold species in the genus Aspergillus known to produce the toxin ochratoxin A, one of the most abundant food-contaminating mycotoxins, and citrinin. It also produces the dihydroisocoumarin mellein. It is a filamentous fungus in nature and has characteristic biseriate conidiophores. Traditionally a soil fungus, has now began to adapt to varied ecological niches, like agricultural commodities, farmed animal and marine species. In humans and animals the consumption of this fungus produces chronic neurotoxic, immunosuppressive, genotoxic, carcinogenic and teratogenic effects. Its airborne spores are one of the potential causes of asthma in children and lung diseases in humans. The pig and chicken populations in the farms are the most affected by this fungus and its mycotoxins. Certain fungicides like mancozeb, copper oxychloride, and sulfur have inhibitory effects on the growth of this fungus and its mycotoxin producing capacities.
A c11 alpha-hydroxylase mutant with improved catalytic activity, methods and uses thereof
The application belongs to the technical field of genetic engineering and enzyme engineering, and discloses a C11 alpha-hydroxylase mutant D118V with improved catalytic activity, wherein the amino acid sequence is shown as SEQ ID NO. 4, and the corresponding nucleotide sequence is shown as SEQ ID NO. 5. The C11 alpha-hydroxylase from Aspergillus ochraceus is subjected to site-directed saturation mutation, and the C11 alpha-hydroxylase mutant D118V for improving the yield of 11 alpha, 17 alpha-dihydroxy progesterone is obtained by using a Saccharomyces cerevisiae expression platform. When the concentration of the substrate 17 alpha-hydroxy progesterone is 2 g / L, the concentration of 11 alpha, 17 alpha-dihydroxy progesterone after 36 h of conversion is 1.19 g / L, and the production intensity is 758.15 mg / (L·d), which is increased by 16.67% and 172.77% compared with the wild-type CYP68J5, thereby providing an application case and basic data support for the modification of steroid C11 alpha-hydroxylase.
Owner:TIANJIN UNIV OF SCI & TECH
A method for detecting ochratoxin a in milk based on a dual recognition electrochemical sensor
The application discloses a method for detecting ochratoxin A in milk based on a double-identification electrochemical sensor, and compared with other OTA detection methods, the sensor has a wider linear range (0-300nM) and a lower LOD (0.09nM) under optimal conditions; the sensor has high repeatability, stability and specificity, and the sensor has good feasibility in detection of different types of milk samples, and a good recovery rate of 95.4%-105.6% is obtained.
Owner:SHAANXI NORMAL UNIV
A method for controlling the biotransformation of canlinone by monitoring hydroxylase gene expression.
This invention discloses a method for controlling the biotransformation of canlione by monitoring the expression level of hydroxylase gene. The steps are as follows: using *Aspergillus ochraceus* as the inoculum and canlione as the substrate, the expression level of the hydroxylase gene is monitored using real-time quantitative PCR during cell culture and transformation to determine the mid-logarithmic growth phase of the cell culture; canlione is then biotransformed again using *Aspergillus ochraceus* as the inoculum and canlione as the substrate, with an exogenous inducer added during the mid-logarithmic growth phase of the cell culture to improve the transformation rate. This method uses the hydroxylase gene expression level, a molecular biological indicator, as a key process parameter to guide the decision-making of substrate addition timing and the optimization of transformation conditions. It enables precise control of process optimization driven by gene expression data, achieving a significant increase in transformation rate and a significant reduction in transformation cycle, showing promising application prospects.
Owner:SHANGHAI INST OF TECH +1
Double-enzyme sensing system for detecting ochratoxin A and application of double-enzyme sensing system
PendingCN122071735AMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementPhenylalanine dehydrogenaseDegradative enzyme
The invention discloses a double-enzyme sensing system for detecting ochratoxin A and application of the double-enzyme sensing system. The invention provides a method for detecting OTA in a to-be-detected sample, which comprises the following steps: A1) carrying out hydrolysis reaction on the to-be-detected sample by using amide hydrolase to obtain a first-step reaction solution; a2) adding phenylalanine dehydrogenase, coenzyme NAD < + > and Glycine into the first-step reaction solution, and reacting again to obtain a second-step reaction solution; adding a color developing agent into the second-step reaction solution, and carrying out color developing reaction to obtain a reaction product; detecting OTA in the sample to be detected according to the reaction product; according to the invention, a novel OTA degrading enzyme ThADH of an OTA sensing element and a product L-phenylalanine sensing element phenylalanine dehydrogenase are combined, and efficient, visual and quantitative detection of OTA is realized by using a color reaction in a product catalysis process.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI