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10 results about "Aspergillus ochraceus" patented technology
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Aspergillus ochraceus is a mold species in the genus Aspergillus known to produce the toxin ochratoxin A, one of the most abundant food-contaminating mycotoxins, and citrinin. It also produces the dihydroisocoumarin mellein. It is a filamentous fungus in nature and has characteristic biseriate conidiophores. Traditionally a soil fungus, has now began to adapt to varied ecological niches, like agricultural commodities, farmed animal and marine species. In humans and animals the consumption of this fungus produces chronic neurotoxic, immunosuppressive, genotoxic, carcinogenic and teratogenic effects. Its airborne spores are one of the potential causes of asthma in children and lung diseases in humans. The pig and chicken populations in the farms are the most affected by this fungus and its mycotoxins. Certain fungicides like mancozeb, copper oxychloride, and sulfur have inhibitory effects on the growth of this fungus and its mycotoxin producing capacities.
Phytobacterium plantarum capable of inhibiting growth of monascus and application of phytobacterium plantarum
PendingCN121975682AGood antibacterial effectMold does not appearFungiBacteriaBiotechnologyAntibacterial activity
The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus capable of inhibiting growth of monascus and application of the plant lactobacillus. The preservation number of the plant lactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.34851. According to the present invention, the plant lactobacillus CCNH295 has strong bacterial inhibition ability on common soybean meal contaminating molds, such as aspergillus flavus, aspergillus ochraceus, fusarium moniliforme and penicillium; the plant lactobacillus CCNH295 has excellent bacteriostatic ability to monascus, and when the plant lactobacillus CCNH295 is used for fermentation production of soybean meal, growth of monascus can be remarkably inhibited, so that mildewing of the fermented soybean meal is effectively inhibited, and the prepared fermented soybean meal does not mildew after being placed at 37 DEG C for 6 months; besides, the fermented soybean meal produced by solid state fermentation of the plant lactobacillus CCNH295 is used as a feed raw material, so that the stability of the feed can be improved, and the palatability of the feed can be improved.
Owner:COFCO NUTRITION AND HEALTH RESEARCH INSTITUTE CO LTD +1
Bacillus subtilis, complex microbial inoculant and application of bacillus subtilis and complex microbial inoculant in degradation of mycotoxin
The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.
Owner:SHANDONG BAOLAI-LEELAI BIOENGINEERING CO LTD (CN)
Lysinibacillus bacilli B1 and application thereof
The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.
Owner:NORTHWEST INST OF PLATEAU BIOLOGY CHINESE ACAD OF SCI
A c11 alpha-hydroxylase mutant with improved catalytic activity, methods and uses thereof
The application belongs to the technical field of genetic engineering and enzyme engineering, and discloses a C11 alpha-hydroxylase mutant D118V with improved catalytic activity, wherein the amino acid sequence is shown as SEQ ID NO. 4, and the corresponding nucleotide sequence is shown as SEQ ID NO. 5. The C11 alpha-hydroxylase from Aspergillus ochraceus is subjected to site-directed saturation mutation, and the C11 alpha-hydroxylase mutant D118V for improving the yield of 11 alpha, 17 alpha-dihydroxy progesterone is obtained by using a Saccharomyces cerevisiae expression platform. When the concentration of the substrate 17 alpha-hydroxy progesterone is 2 g / L, the concentration of 11 alpha, 17 alpha-dihydroxy progesterone after 36 h of conversion is 1.19 g / L, and the production intensity is 758.15 mg / (L·d), which is increased by 16.67% and 172.77% compared with the wild-type CYP68J5, thereby providing an application case and basic data support for the modification of steroid C11 alpha-hydroxylase.
Owner:TIANJIN UNIV OF SCI & TECH
A method for detecting ochratoxin a in milk based on a dual recognition electrochemical sensor
The application discloses a method for detecting ochratoxin A in milk based on a double-identification electrochemical sensor, and compared with other OTA detection methods, the sensor has a wider linear range (0-300nM) and a lower LOD (0.09nM) under optimal conditions; the sensor has high repeatability, stability and specificity, and the sensor has good feasibility in detection of different types of milk samples, and a good recovery rate of 95.4%-105.6% is obtained.
Owner:SHAANXI NORMAL UNIV
A method for controlling the biotransformation of canlinone by monitoring hydroxylase gene expression.
This invention discloses a method for controlling the biotransformation of canlione by monitoring the expression level of hydroxylase gene. The steps are as follows: using *Aspergillus ochraceus* as the inoculum and canlione as the substrate, the expression level of the hydroxylase gene is monitored using real-time quantitative PCR during cell culture and transformation to determine the mid-logarithmic growth phase of the cell culture; canlione is then biotransformed again using *Aspergillus ochraceus* as the inoculum and canlione as the substrate, with an exogenous inducer added during the mid-logarithmic growth phase of the cell culture to improve the transformation rate. This method uses the hydroxylase gene expression level, a molecular biological indicator, as a key process parameter to guide the decision-making of substrate addition timing and the optimization of transformation conditions. It enables precise control of process optimization driven by gene expression data, achieving a significant increase in transformation rate and a significant reduction in transformation cycle, showing promising application prospects.
Owner:SHANGHAI INST OF TECH +1
Aspergillus ochraceus strain and application thereof
The invention discloses an Aspergillus ochraceus Slt L1 strain and application thereof. The Aspergillus ochraceus Slt L1 strain has the morphological characteristics that after the Aspergillus ochraceus Slt L1 strain is cultured on a PDA culture medium for 7 days, typical Aspergillus ochraceus colony characteristics are formed, hyphae are separated and branched, conidiophore is upright, and conidia are spherical to nearly spherical. The prodenia litura strain has obvious pathogenicity to prodenia litura eggs, can lead to 100% of egg death rate, and has wide metabolic adaptability and environmental tolerance. Through the determination of a Biolog phenotype chip system, the bacterium can efficiently utilize a carbon source, a nitrogen source, a phosphorus source, a sulfur source and a plurality of biosynthetic pathways, and can keep the growth activity under the extreme conditions of osmotic pressure and pH. The microbial inoculum can be used for biological control of prodenia litura, and has the characteristics of greenness, high efficiency, environmental friendliness and the like.
Owner:GUIZHOU TOBACCO SCI RES INST
Efficient detoxification of neo-sphingobacterium on ochratoxin A and application of neo-sphingobacterium
The invention relates to the technical field of microorganisms, and discloses a novel sphingobacterium sp. And application thereof, the novel sphingobacterium sp. Is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 67028. The invention also provides a biological detoxication agent containing the preserved strain and a preparation method of the biological detoxication agent. In addition, the invention also provides an application of the strain or the biological detoxication agent in degrading ochratoxin A, and an application of the strain or the biological detoxication agent in inhibiting aspergillus ochraceus growth and toxin production.
Owner:FOSHAN UNIVERSITY
Double-enzyme sensing system for detecting ochratoxin A and application of double-enzyme sensing system
PendingCN122071735AMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementPhenylalanine dehydrogenaseDegradative enzyme
The invention discloses a double-enzyme sensing system for detecting ochratoxin A and application of the double-enzyme sensing system. The invention provides a method for detecting OTA in a to-be-detected sample, which comprises the following steps: A1) carrying out hydrolysis reaction on the to-be-detected sample by using amide hydrolase to obtain a first-step reaction solution; a2) adding phenylalanine dehydrogenase, coenzyme NAD < + > and Glycine into the first-step reaction solution, and reacting again to obtain a second-step reaction solution; adding a color developing agent into the second-step reaction solution, and carrying out color developing reaction to obtain a reaction product; detecting OTA in the sample to be detected according to the reaction product; according to the invention, a novel OTA degrading enzyme ThADH of an OTA sensing element and a product L-phenylalanine sensing element phenylalanine dehydrogenase are combined, and efficient, visual and quantitative detection of OTA is realized by using a color reaction in a product catalysis process.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Composite material with toxin-producing fungus resisting activity and fungaltoxin degrading effect
The invention provides a composite material with both toxin-producing fungus resisting activity and fungaltoxin degrading effect. According to the invention, montmorillonite is taken as a carrier, and the advantages of bacillus, trichoderma reesei extracellular enzyme, cinnamyl aldehyde and montmorillonite are combined and compounded into a composite material with both toxin-producing fungus activity resistance and mycotoxin degradation effect, so that the composite material has both toxin-producing fungus inhibition and mycotoxin degradation effects; the compound can be applied to feed and grain products to inhibit mildew and reduce mycotoxin pollution. When the composite material is added into the corn flour polluted by toxin-producing bacteria and mycotoxin, the composite material can significantly inhibit the growth of toxin-producing fungi (fusarium graminearum, fusarium equiseti, aspergillus flavus and aspergillus ochraceus) in the corn flour, and the AFB1 pollution inhibition rate and the ZEN pollution inhibition rate are 98.89% and 93.54% respectively.
Owner:SHANDONG ACADEMY OF AGRICULTURAL SCIENCES