31 results about "Peronophythora litchii" patented technology

The invention discloses an application of a volatile substance produced by streptomyces fimicarius in control of plant diseases. Particularly, the volatile substance produced by streptomyces fimicarius can inhibit growth of 15 phytopathogens, has the highest inhibition function on peronophythora litchii, pythium myriotylum, alternaria alternate, fusarium graminearum, colletotrichum musarum and pyricularia grisea and has the inhibition rate up to 100%. The invention further makes clear that the volatile substance produced by streptomyces fimicarius contains 7 compounds with remarkable bacteriostasis, and a direction is provided for future studies of control drugs for peronophythora litchii, pythium myriotylum, alternaria alternate, colletotrichum musarum, pyricularia grisea and other phytopathogens.

The invention discloses a germicide composition containing coumoxystrobin, which comprises effective active ingredients consisting of a first active ingredient and a second active ingredient, wherein the first active ingredient is coumoxystrobin, the second active ingredient is a bactericide preventing and treating oomycete diseases and selected from dimethomorph, metalaxyl, metalaxyl-M, benalaxyl, propamocarb, fosetyl aluminium, cymoxanil, cyazofamid or fluopicolide, and the weight ratio of the first active ingredient to the second active ingredient is 1:20-20:1. The composition can be prepared into water dispersible granules, wettable powder, suspending agents and flowable concentrates for seed coating permitted in agriculture. The composition disclosed by the invention has reasonable components, a good bactericidal effect and low medication cost, and the activity and the bactericidal effect of the composition are not the simple sum of the activity of each component. Compared with the existing single preparation, the composition also has an obvious synergistic effect in addition to an obvious sterilizing effect, the generation of resistance is retarded, the residual quantity of the preparation is small, and the composition has no pollution on crops, has good safety and meets the requirement on the safety of the pesticide preparation. The germicide composition disclosed by the invention has obvious prevention and treatment effects on vegetable downy mildew, vegetable epidemics, vegetable and cotton seedling damping off, fruit tree downy mildew and peronophythora litchii.

The invention relates to a compound as well as a preparation method and an application thereof, in particular to a preparation method of a novel medicament for inhibiting pestalotiopsis, peronophythora litchii and cylindrocladium parasiticum. Naphthylacetic acid technical, bromoethanol technical and carbendazim technical as synthesis raw materials are subjected to a reaction, and naphthylacetic acid-carbendazim conjugate is obtained and is the final product. The invention also discloses nuclear magnetic resonance structural data, hydrogen spectra and carbon spectra of the novel compound. The compound has good bacteriostatic effects on pestalotiopsis, peronophythora litchii and cylindrocladium parasiticum.

The invention discloses application of mycophenolic acid in inhibiting Peronophythora litchii. It is concluded through reaches for the first time that mycophenolic acid has an important use in preventing and treating Peronophythora litchii, particularly inhibiting growth of mycelia of Peronophythora litchii and emergence of sporangium of Peronophythora litchii, thereby effectively inhibiting occurrence of Peronophythora litchii. In addition, mycophenolic acid can inhibit the growth of pathogens but can never completely kill them, and it is indicated that mycophenolic acid has low toxicity andis more environmentally friendly. Therefore, the mycophenolic acid has a promising application prospect in preventing and treating Peronophythora litchii.

The invention discloses a peronophythora litchii LAMP (loop-mediated isothermal amplification) primer and a rapid detection method thereof which are specially applied to specific detection of peronophythora litchii. The peronophythora litchii LAMP primer is mainly adopted and designed, and through isothermal amplification visual inspection or agarose gel electrophoresis detection, green fluorescence can be observed or an LAMP characteristic ladder can appear. The LAMP primer and a usage method thereof can be applied to rapid, sensitive and accurate detection of the peronophythora litchii in plants infected with the peronophythora litchii in production practice, can also be applied to early diagnosis of diseases and pests in fields as well as monitoring and identification of germs, and provide reliable technical and theoretical bases for control of diseases and pests caused by the peronophythora litchii.

The invention provides a cultivation method of disease-resistant homozygote litchi trees. According to the cultivation method, after flower buds are treated with a nutrient solution, complete haploidplants are cultured through anther, the haploid plants are inoculated into a peronophythora litchii culture medium, and disease-resistant plants are screened out; and colchicine is adopted to treat the disease-resistant plants to obtain homozygote plants, then the homozygote plants are subjected to selfing, and the homozygote plants which are consistent in character, excellent in quality and goodin disease resistance are screened out. According to the cultivation method, a tissue culture technology is adopted to culture the haploid plants with peronophythora litchii resistance, homozygote plants are obtained through chromosome doubling, finally the homozygote plants are subjected to selfing to obtain the homozygote plants with peronophythora litchii resistance, consistent characters, excellent quality and good disease resistance, so that the time for obtaining the disease-resistant homozygote plants by traditional breeding is shortened, and the process is simple and easy to popularize.

The invention discloses peronophythora litchii molecular detection primers and a detection method thereof, and is dedicated to specific molecular detection of peronophythora litchi. A pair of peronophythora litchii specific primers PvYF1:5'-GTCCGAGTTTCTAGCAGATTG-3' and PvYR1:5'-ACGATAATACCGTGAGCGCC-3' are mainly designed; through PCR amplification and agarose gel electrophoresis, a specific amplification product with the fragment length of 249 bp is specifically amplified in peronophythora litchii pure DNA and peronophythora litchii-carried plants. The specific molecular detection primers and a usage method thereof can be used for rapid, sensitive and specific detection of peronophythora in the peronophythora litchii infected plants in production practice, at the same time, can be used for early diagnosis of field diseases and monitoring and identification of pathogenic bacteria, and provides a reliable technology and theoretical basis for diseases caused by peronophythora litchii.