4 results about "Peronophythora litchii" patented technology

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.

The invention discloses a method for biosynthesizing nano platinum by using space mutant bacteria PS04-17 and application of the method. The invention provides a novel application of a space mutant strain PS04-17 strain in biosynthesis of nano platinum particles. Researches show that the PS04-17 strain has a reduction capability on hexachloroplatinic acid; the method for biologically synthesizing nano platinum by reducing hexachloroplatinic acid in a liquid fermentation culture medium by using the PS04-17 strain is simple and convenient, mild in condition, low in cost and environment-friendly. The invention provides more new strain resources with high reduction efficiency on hexachloroplatinic acid, and fills the blank in the field of synthesis of nano platinum by microorganisms. Meanwhile, the PS04-17 synthesized nano platinum can prevent and control various plant pathogens, the bacteriostasis rate of the PS04-17 synthesized nano platinum on colletotrichum litchii and peronophythora litchii can reach 100%, the PS04-17 synthesized nano platinum can also be used for preventing and controlling wheat stem rot, the prevention and control efficiency reaches 78.2%, and a new solution is provided for prevention and control of crop diseases.

The application discloses a gene PlIRP1 and application thereof in prevention and treatment of litchi blight, and belongs to the technical field of green prevention and control of crop diseases.The application applies a protoplast transformation technology based on polyethylene glycol and a CRISPR / Cas9 knockout strategy, constructs a PlIRP1 knockout vector, and uses PEG-mediated protoplast transformation and a homologous recombination method to knockout the gene PlIRP1 from litchi blight Peronophythora litchii, so as to obtain a knockout mutant.Compared with a wild type, the mutant has obviously weakened pathogenicity and oospore production.The application proves that PlIRP1 is necessary for sexual compatibility and infection pathogenicity of the litchi blight Peronophythora litchii.The research is helpful to further clarify the pathogenic molecular mechanism of the litchi blight Peronophythora litchii, and provides a target gene for development of an effective fungicide.

The present application provides an effective and safe litchi endogenous polypeptide and its application in controlling plant diseases. The amino acid sequence of the litchi endogenous polypeptide P2 is shown as SEQ ID NO. 1. After the litchi endogenous polypeptide P2 is used to treat litchi, the resistance of litchi fruits to litchi blight Peronophythora litchii can be improved. Therefore, the litchi endogenous polypeptide P2 can be used to prepare a novel preservative and a plant disease resistance inducer against fungal diseases, and is applied in the field of plant fungal disease prevention and control.