11 results about "Peronophythora litchii" patented technology

The invention belongs to the technical field of green biological prevention and control of plant diseases, and particularly relates to a biopesticide based on coumarin metabolites and application of the biopesticide in green prevention and control of litchi downy blight. A novel litchi downy blight prevention and control method is developed based on coumarin compounds, and the method passes through in-vitro bacteriostasis experiments, fresh leaf bacteriostasis experiments and comprehensive evaluation of the prevention and control effect. According to the present invention, it is found that the three coumarins such as Scopoletin, Esculin and Esculin can significantly inhibit the growth of peronophythora litchii on a culture medium and a living body leaf, and have good prevention and control effects, and the Scopoletin and Esculin have the most significant antibacterial effect, and can synergistically enhance the inhibition effect on peronophythora litchii, such that the Scopoletin, Esculin and Esculin can significantly inhibit the growth of peronophythora litchii. The method has the advantages of environmental protection, high efficiency, safety and economy, is suitable for large-scale application, and provides a new solution for the prevention and treatment of the litchi downy blight.

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.

The invention relates to a biological agent effectiveness evaluation method and system for litchi pest analysis, and belongs to the technical field of litchi pest prevention and control. Pest situation data in a litchi garden area are determined, and then metabolites of a target pest situation type are collected according to the pest situation data in the litchi garden area; metabolite of a target insect situation type is analyzed, detoxification enzyme change data of pests is collected, accordingly, biological agent component types are screened according to the detoxification enzyme change data of the pests, finally, prevention and control effectiveness analysis is conducted on the biological agent component types, and the final biological agent component type is recommended according to an analysis result. According to the present invention, by analyzing the detoxification enzyme change data of the pests in the pest metabolites in the lychee garden region, the biological agent component type is formulated according to the detoxification enzyme change data of the pests in the pest metabolites in the lychee garden region, such that the pests are effectively controlled, and the pest control effect is improved.

The invention discloses a method for biosynthesizing nano platinum by using space mutant bacteria PS04-17 and application of the method. The invention provides a novel application of a space mutant strain PS04-17 strain in biosynthesis of nano platinum particles. Researches show that the PS04-17 strain has a reduction capability on hexachloroplatinic acid; the method for biologically synthesizing nano platinum by reducing hexachloroplatinic acid in a liquid fermentation culture medium by using the PS04-17 strain is simple and convenient, mild in condition, low in cost and environment-friendly. The invention provides more new strain resources with high reduction efficiency on hexachloroplatinic acid, and fills the blank in the field of synthesis of nano platinum by microorganisms. Meanwhile, the PS04-17 synthesized nano platinum can prevent and control various plant pathogens, the bacteriostasis rate of the PS04-17 synthesized nano platinum on colletotrichum litchii and peronophythora litchii can reach 100%, the PS04-17 synthesized nano platinum can also be used for preventing and controlling wheat stem rot, the prevention and control efficiency reaches 78.2%, and a new solution is provided for prevention and control of crop diseases.

The invention discloses a Burkholderia polluting strain LZ816 and an application of the Burkholderia polluting strain LZ816 in the aspect of preventing and treating litchi diseases, and particularly discloses a Burkholderia polluting strain LZ816 and an application of the Burkholderia polluting strain LZ816. The polluted burkholderia sp. LZ816 is preserved in Guangdong Microbial Culture Collection Center on March 4, 2025, and the preservation number is GDMCC No: 65968. The bacterium and a fermentation product of the bacterium have relatively good bacteriostatic activity on peronophythora litchii and colletotrichum frutescens, and can be used for preventing and treating common diseases of litchi, such as peronophythora litchii, anthracnose of litchi and the like. The bacterium can be used for preventing and treating common diseases of litchi, such as peronophythora litchii and colletotrichum frutescens. The burkholderia sp. LZ816 disclosed by the invention can be used for preparing a novel, efficient and broad-spectrum biocontrol microbial inoculum, and the burkholderia sp. Is high in propagation speed, low in requirement on nutrition, high in colonization capability and high in application value.

The invention discloses an InDel marker closely linked with resistance to peronophythora litchii and application of the InDel marker, and belongs to the technical field of plant molecular breeding, and the marker is a 773 bp insertion fragment in an LcOMT1 gene. The site in the disease-resistant litchi variety is of a heterozygous insertion genotype, and the site in the susceptible variety is of a homozygous deletion genotype. The invention further discloses the function of the LcOMT1 gene. The LcOMT1 is overexpressed in hairy roots of infected litchi materials, the resistance of plants to downy blight can be remarkably enhanced, and a key target is provided for litchi disease-resistant gene engineering. The OMT1 molecular marker is closely linked with the litchi downy blight resistance character, and can be used for accurate screening of resistant germplasm resources and molecular marker assisted breeding.

The application discloses a gene PlIRP1 and application thereof in prevention and treatment of litchi blight, and belongs to the technical field of green prevention and control of crop diseases.The application applies a protoplast transformation technology based on polyethylene glycol and a CRISPR / Cas9 knockout strategy, constructs a PlIRP1 knockout vector, and uses PEG-mediated protoplast transformation and a homologous recombination method to knockout the gene PlIRP1 from litchi blight Peronophythora litchii, so as to obtain a knockout mutant.Compared with a wild type, the mutant has obviously weakened pathogenicity and oospore production.The application proves that PlIRP1 is necessary for sexual compatibility and infection pathogenicity of the litchi blight Peronophythora litchii.The research is helpful to further clarify the pathogenic molecular mechanism of the litchi blight Peronophythora litchii, and provides a target gene for development of an effective fungicide.

The present application provides an effective and safe litchi endogenous polypeptide and its application in controlling plant diseases. The amino acid sequence of the litchi endogenous polypeptide P2 is shown as SEQ ID NO. 1. After the litchi endogenous polypeptide P2 is used to treat litchi, the resistance of litchi fruits to litchi blight Peronophythora litchii can be improved. Therefore, the litchi endogenous polypeptide P2 can be used to prepare a novel preservative and a plant disease resistance inducer against fungal diseases, and is applied in the field of plant fungal disease prevention and control.

The invention provides a natural phenolic compound thymol for inhibiting peronophythora litchii, and further provides an application of the thymol in preventing and treating the peronophythora litchii. The natural phenolic compound thymol can be used for preventing and treating the peronophythora litchii. In-vitro bacteriostasis experiments and prevention and control experiments prove that thymol has remarkable inhibitory activity on peronophythora litchii and can effectively reduce the incidence rate of peronophythora litchii. The thymol is used as an active component for preparing the biopesticide or the plant source bacteriostatic agent for preventing and treating the litchi peronophythora blight for the first time, and the thymol has the characteristics of high efficiency, low toxicity and environment friendliness, can replace a traditional chemical bactericide and can reduce pesticide residues and pathogenic bacterium drug resistance.

This invention provides the natural phenolic compound thymol in inhibiting Phytophthora downy mildew (Phytophthora indicum). Peronophythora litchii This invention also provides the application of thymol in the control of litchi downy mildew. In vitro antibacterial experiments and control tests confirmed that thymol has significant inhibitory activity against litchi downy mildew and can effectively reduce the incidence of litchi downy mildew. This invention is the first to propose using thymol as an active ingredient to prepare a biological pesticide or plant-derived antifungal agent for the control of litchi downy mildew. It features high efficiency, low toxicity, and environmental friendliness, and can replace traditional chemical fungicides, reducing pesticide residues and pathogen resistance problems.

The invention provides an effective and safe litchi endogenous polypeptide and application thereof in controlling plant diseases. The amino acid sequence of the litchi endogenous polypeptide P2 is as shown in SEQ ID NO. 1. After litchi is treated by the litchi endogenous polypeptide P2, the resistance of litchi fruits to peronophythora litchii can be improved. Therefore, the litchi endogenous polypeptide P2 can be used for preparing a novel antifungal disease preservative and a plant disease-resistant inducer, and is applied to the field of prevention and control of plant fungal diseases.