31 results about "Peronophythora litchii" patented technology

The invention discloses application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii, provides application of pterostilbene or a solution thereof to inhibition of peronophythora litchii and also provides application of pterostilbene or a solution thereof to prevention and treatment of peronophythora litchii. Experiments show that pterostilbene can be used for preventing and treating botrytis cinerea and peronophythora litchii of harvested grapes and litchis; and pterostilbene can be separately used, can also be combined with other antibacterial agents for improving the antibacterial effect or reducing the toxic and side effects, is green and safe, is beneficial to human health, meets the food safety requirements and achieves the effects of preventing and treating botrytis cinerea and peronophythora litchi and retaining freshness of fruits.

The invention discloses a composition for treating peronophythora litchii based on guaiacol and an application method thereof. Guaiacol is applied to the agricultural field and prepared into the composition together with dimethomorph according to a certain proportion to be used for treating peronophythora litchii. The new application of guaiacol is developed, the peronophythora litchii can be effectively treated, disease resistance of crops is improved, and compared with existing other single pesticides, the composition is better in control effect, low in production cost and worth popularizing.

The invention belongs to the technical field of plant disease biocontrol, and discloses a strain of bacillus altitudinis XJGS-4 and an application thereof in prevention and treatment of peronophythoralitchii. The bacillus altitudinis is named as bacillus altitudinis XJGS-4, is preserved in Guangdong Microbial Culture Collection Center (GDMCC for short) at Guangdong Institute of Microbiology, 5 floor, No.59 building, No.100 courtyard, Xianlie Middle Road, Guangzhou City, has the preservation number of GDMCC No:60698, and is preserved on June 17, 2019. The obtained bacillus altitudinis comes from rhizosphere soil of healthy litchi chinensis sonn fruit trees, is mutually symbiotic with litchi chinensis sonn for a long time, and avoids the possibility of damaging of the stability of a micro ecological environment on a plant surface; the bacillus altitudinis and a biological preparation based on the bacillus altitudinis have a strong inhibitory effect on peronophythora litchii, and have agood prospect of development and application.

The invention discloses a peronophythora litchii secreting type protein elicitor PlPeL8 and an application thereof. The amino acid sequence of the protein elicitor PlPeL8 is as shown in SEQ ID NO.1, the coding gene of the protein elicitor PlPeL8 is constructed into a plant expression vector, then agrobacterium tumefaciens is transformed, transient expression is carried out in nicotiana benthamiana cells, and it is found that the protein elicitor PlPeL8 can induce plant defense reactions including causing plant cell allergic necrosis, inducing plant cell apoptosis and inducing plant cell apoptosis. The gene can induce active oxygen bursting and up-regulated expression of marker genes of plant immune related pathways such as salicylic acid and jasmonic acid pathways, can significantly improve the resistance of plants to phytophthora, provides a new way for improving plant disease resistance and reducing diseases, and has a wide application prospect in green development of modern agriculture.

The invention discloses an application of a volatile substance produced by streptomyces fimicarius in control of plant diseases. Particularly, the volatile substance produced by streptomyces fimicarius can inhibit growth of 15 phytopathogens, has the highest inhibition function on peronophythora litchii, pythium myriotylum, alternaria alternate, fusarium graminearum, colletotrichum musarum and pyricularia grisea and has the inhibition rate up to 100%. The invention further makes clear that the volatile substance produced by streptomyces fimicarius contains 7 compounds with remarkable bacteriostasis, and a direction is provided for future studies of control drugs for peronophythora litchii, pythium myriotylum, alternaria alternate, colletotrichum musarum, pyricularia grisea and other phytopathogens.

The invention discloses application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii, provides application of pterostilbene or a solution thereof to inhibition of peronophythora litchii and also provides application of pterostilbene or a solution thereof to prevention and treatment of peronophythora litchii. Experiments show that pterostilbene can be used for preventing and treating botrytis cinerea and peronophythora litchii of harvested grapes and litchis; and pterostilbene can be separately used, can also be combined with other antibacterial agents for improving the antibacterial effect or reducing the toxic and side effects, is green and safe, is beneficial to human health, meets the food safety requirements and achieves the effects of preventing and treating botrytis cinerea and peronophythora litchi and retaining freshness of fruits.

The invention discloses a germicide composition containing coumoxystrobin, which comprises effective active ingredients consisting of a first active ingredient and a second active ingredient, wherein the first active ingredient is coumoxystrobin, the second active ingredient is a bactericide preventing and treating oomycete diseases and selected from dimethomorph, metalaxyl, metalaxyl-M, benalaxyl, propamocarb, fosetyl aluminium, cymoxanil, cyazofamid or fluopicolide, and the weight ratio of the first active ingredient to the second active ingredient is 1:20-20:1. The composition can be prepared into water dispersible granules, wettable powder, suspending agents and flowable concentrates for seed coating permitted in agriculture. The composition disclosed by the invention has reasonable components, a good bactericidal effect and low medication cost, and the activity and the bactericidal effect of the composition are not the simple sum of the activity of each component. Compared with the existing single preparation, the composition also has an obvious synergistic effect in addition to an obvious sterilizing effect, the generation of resistance is retarded, the residual quantity of the preparation is small, and the composition has no pollution on crops, has good safety and meets the requirement on the safety of the pesticide preparation. The germicide composition disclosed by the invention has obvious prevention and treatment effects on vegetable downy mildew, vegetable epidemics, vegetable and cotton seedling damping off, fruit tree downy mildew and peronophythora litchii.

The invention relates to a compound as well as a preparation method and an application thereof, in particular to a preparation method of a novel medicament for inhibiting pestalotiopsis, peronophythora litchii and cylindrocladium parasiticum. Naphthylacetic acid technical, bromoethanol technical and carbendazim technical as synthesis raw materials are subjected to a reaction, and naphthylacetic acid-carbendazim conjugate is obtained and is the final product. The invention also discloses nuclear magnetic resonance structural data, hydrogen spectra and carbon spectra of the novel compound. The compound has good bacteriostatic effects on pestalotiopsis, peronophythora litchii and cylindrocladium parasiticum.

The invention discloses application of mycophenolic acid in inhibiting Peronophythora litchii. It is concluded through reaches for the first time that mycophenolic acid has an important use in preventing and treating Peronophythora litchii, particularly inhibiting growth of mycelia of Peronophythora litchii and emergence of sporangium of Peronophythora litchii, thereby effectively inhibiting occurrence of Peronophythora litchii. In addition, mycophenolic acid can inhibit the growth of pathogens but can never completely kill them, and it is indicated that mycophenolic acid has low toxicity andis more environmentally friendly. Therefore, the mycophenolic acid has a promising application prospect in preventing and treating Peronophythora litchii.

The invention provides a method for simply, conveniently and rapidly separating peronophythora litchii. The method comprises the following steps: (1) selecting and disinfecting disease tissues of litchi; (2) preparing a V8 vegetable culture medium, adding benzyl ampicillin, prochloraz, hymexazol and a growth promoting agent, and mixing evenly to obtain a selective culture medium, wherein the growth promoting agent is formed by mixing beta-sitosterol mother liquor, litchi rind ground-out liquid and water; (3) culturing the disease tissues in the selective culture medium, selecting hyphae, conducting microscopic examination, and inoculating the hyphae to a new selective culture medium for culturing; and (4) cutting hypha blocks from a microorganism colony, adding the litchi rind ground-out liquid for soaking, and culturing in a new V8 vegetable culture medium to obtain peronophythora litchii. The peronophythora litchii obtained through separation has good activity and pathogenicity. The method is easy to operate, has a high separation rate, is easy for separation, and can realize efficient separation of the peronophythora litchii.

The invention discloses a peronophythora litchii LAMP (loop-mediated isothermal amplification) primer and a rapid detection method thereof which are specially applied to specific detection of peronophythora litchii. The peronophythora litchii LAMP primer is mainly adopted and designed, and through isothermal amplification visual inspection or agarose gel electrophoresis detection, green fluorescence can be observed or an LAMP characteristic ladder can appear. The LAMP primer and a usage method thereof can be applied to rapid, sensitive and accurate detection of the peronophythora litchii in plants infected with the peronophythora litchii in production practice, can also be applied to early diagnosis of diseases and pests in fields as well as monitoring and identification of germs, and provide reliable technical and theoretical bases for control of diseases and pests caused by the peronophythora litchii.

The invention provides a cultivation method of disease-resistant homozygote litchi trees. According to the cultivation method, after flower buds are treated with a nutrient solution, complete haploidplants are cultured through anther, the haploid plants are inoculated into a peronophythora litchii culture medium, and disease-resistant plants are screened out; and colchicine is adopted to treat the disease-resistant plants to obtain homozygote plants, then the homozygote plants are subjected to selfing, and the homozygote plants which are consistent in character, excellent in quality and goodin disease resistance are screened out. According to the cultivation method, a tissue culture technology is adopted to culture the haploid plants with peronophythora litchii resistance, homozygote plants are obtained through chromosome doubling, finally the homozygote plants are subjected to selfing to obtain the homozygote plants with peronophythora litchii resistance, consistent characters, excellent quality and good disease resistance, so that the time for obtaining the disease-resistant homozygote plants by traditional breeding is shortened, and the process is simple and easy to popularize.

The invention discloses a method for detecting litchi peronophythora blight resistance of litchis and application of the method, and the method comprises the following steps: inoculating peronophythora blight spore suspension on each fresh leaf of the litchis; culturing the inoculated fresh litchi leaves at a constant temperature for 2-4 days; and detecting whether the litchi resists the peronophythora litchii or not according to the disease index of the fresh litchi leaves infected by the peronophythora litchii. The detection method disclosed by the invention is accurate and reliable, can be used for carrying out peronophythora litchii resistance phenotype detection on litchi materials in a large scale in multiple periods in one year, is short in time consumption and low in cost, and can be widely applied to disease-resistant breeding of litchis.

A disclosed sterilization composition containing cyazofamid and a strobilurin compound comprises a component A and a component B with a weight ratio of 1%-50%:1%-50%, wherein the active component A is cyazofamid, and the active component B is any one bactericides selected from azoxystrobin, kresoxim-methyl, ZJ0712 (2-[3-(2,5-diMethyl-phenoxymethyl)-phenyl]-3-methoxy-acrylic acid methyl ester), picoxystrobin and enestroburin. The composition provided by the invention is capable of controlling multiple crop diseases, has obvious synergic effect, is enlarged in sterilization spectrum, and has relatively high activity on downy mildew, peronophythora litchii Chen ex Ko et al., eqpidemic disease, anthracnose and powdery mildew; and the pesticide usage amount is reduced, the residual quantity of the pesticide on crops is reduced, and the environmental pollution is mitigated.

The invention discloses a monitoring and early warning method, system and storage medium for litchi pests based on the Internet of Things. By acquiring litchi plant image information, performing image segmentation to obtain leaf and fruit area image information; processing, constructing a litchi pest recognition model based on generative confrontation network and convolutional neural network, and importing the preprocessed leaf and fruit area image information into the litchi pest recognition model to identify and classify litchi pests; According to the main insect status information of litchi, the classification and early warning of litchi pests are carried out, and at the same time, the comprehensive prevention and control plan is formulated according to the litchi pest type information and the main insect status information, and the litchi pest identification results, pest early warning and comprehensive prevention and control plan are displayed according to the preset method. The invention guarantees the high yield and high quality of the litchi by carrying out graded early warning on the identification of the litchi pests and formulating a targeted prevention and control scheme for the litchi pests.

The invention discloses peronophythora litchii molecular detection primers and a detection method thereof, and is dedicated to specific molecular detection of peronophythora litchi. A pair of peronophythora litchii specific primers PvYF1:5'-GTCCGAGTTTCTAGCAGATTG-3' and PvYR1:5'-ACGATAATACCGTGAGCGCC-3' are mainly designed; through PCR amplification and agarose gel electrophoresis, a specific amplification product with the fragment length of 249 bp is specifically amplified in peronophythora litchii pure DNA and peronophythora litchii-carried plants. The specific molecular detection primers and a usage method thereof can be used for rapid, sensitive and specific detection of peronophythora in the peronophythora litchii infected plants in production practice, at the same time, can be used for early diagnosis of field diseases and monitoring and identification of pathogenic bacteria, and provides a reliable technology and theoretical basis for diseases caused by peronophythora litchii.