92 results about "Scopoletin" patented technology

The invention relates to an HPLC fingerprint spectrum of Saussurea involucrate and a method for establishing a DNA fingerprint spectrum of the Saussurea involucrate. The invention is characterized in that the HPLC fingerprint spectrum comprises fingerprint spectrums of two extraction positions with different polarities of ethyl acetate and n-butyl alcohol, and the fingerprint spectrum of the extraction position of the ethyl acetate has 20 common peaks, wherein 7 peaks refer to scopoletin, rutoside, isoquercitrin, xylostein, quercetrin, homoplantaginin and hispidulin respectively; the fingerprint spectrum of the extraction position of the n-butyl alcohol has 11 common peaks, wherein 3 peaks refer to bergapten, scopoletin and rutoside; and the DNA fingerprint spectrum consists of an RAPD molecular fingerprint spectrum and an ISSR molecular fingerprint spectrum. The quality and the reality and falsity of the Saussurea involucrate can be universally and effectively detected in the aspects of chemistry and biology by combination of HPLC fingerprint and DNA fingerprint.

The invention relates to a quality standard of Sinopanax formosanus pills. In the quality standard, the physical and chemical distinction with poor specificity in the prior standard is deleted, thin-layer identification of scopoletin is added, a method for determining the content of alkaloid by acid dye colorimetry in the prior standard is cancelled, and the contents of the scopoletin and atropine are determined by liquid chromatography. The method for determining the contents of the atropine and the scopoletin has the advantages that: the operation step is simple; the operation time is shortened compared with the prior art, and the detection time for a batch of products is generally about 2 hours; in the whole operation process, manual operation is less, the error resulted from individual difference is decreased to the lowest degree, and the data is completely viewed and processed by computer software; and the method is accurate, reliable, easy in operation and good in reproduction. The quality standard can be used in the daily quality control of the Sinopanax formosanus pills to further guarantee the inner quality of the products and have great meaning for guaranteeing the safe administration to patients.

The invention discloses an analysis method for simultaneously determining six polyphenol content in flue-cured tobacco. The method comprises the following steps: grinding leaves of flue-cured tobacco, extracting through a methanol solution, analyzing filtrate through a high performance liquid chromatography after filtering, adopting a HSST3 chromatographic column and a diode array detector, wherein the detection wavelength of the detector is 342mm, the reference wavelength is 480nm, the bandwidth of the detection wavelength is 4nm, the bandwidth of the reference wavelength is 100nm, and the flow velocity of the flowing phase is 0.4mL / min; the flowing phase A is aqueous solution of glacial acetic acid, and the flowing phase B is methanol solution of glacial acetic acid, simultaneously determining contents of six polyphenols in the flue-cured tobacco: neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, scopoletin, rutin and kaempferol alcohol-3-rutinoside in a gradient elution manner. The adding standard recovery of the method is between 95.6-109.3%, the detection limit is 3.7-9.9 migrogram / g, the relative standard deviation is 0.5-3.9% (n equals to 4), the relative coefficients of the standard solution are more than 0.9995, and the pre-treatment of the method is simple and the analysis time is short.

The application belongs to the technical field of tobacco metabolomics and particularly relates to a method for detecting polyphenolic compounds in tobacco leaves. The method can simultaneously detectone or more of kaempferol, anthocyanin, delphinidin chloride, rhamnazin, chlorogenic acid, delphinidin glucoside, procyanidine B2, anthocyanin rhamnoside, rutin and scopoletin in tobacco leaves. Themethod comprises: extracting pretreated fresh tobacco leaves to obtain a liquid to be detected and carrying out liquid chromatography-tandem mass spectrometry detection and analysis. Compared with theconventional polyphenol detection and analysis method, the detection method has the advantages of high analysis speed, many research objects, good precision, high adding standard recovery rate and good stability, and has a good practical value and a promotion and application significance.

The invention discloses a method for extracting scopoletin compounds from tobacco. The method is characterized by comprising the following steps of preparing tobacco extracts; performing water dissolution on the tobacco extracts; then, performing extraction; eluting the extraction liquid in a macroporous resin chromatographic column by ethyl alcohol; after eluant is collected, performing reduced pressure concentration; then, performing capillary tube sampling thin-layer chromatography; merging the eluant with the similar ingredients; performing reduced pressure concentration until the materials are dry so that a tobacco polar extract is obtained; merging samples showing brownish black spots by a thin-layer chromatography method; performing reduced pressure concentration; obtaining coarse extracts; uniformly stirring the coarse extracts and silica gel; using a positive phase silica gel column for separation and elution; by using the thin-layer chromatography method, merging flavor matters according to different Rf values in accordance with different polarities to obtain fine extracts; performing positive phase column chromatography on the fine extracts; performing elution; segmenting TLC detection into five parts from A to E; performing reverse phase silica gel column chromatography on the section B; then, feeding the sample to a half preparation chromatography system for separation; under each chromatography condition, maintaining the time for 7.23 minutes; and performing separation to obtain the monomeric compound.

The invention relates to a novel method for preparing Scopoletin, which comprises that: (a) 2,4,5-trimethoxybenzaldehyde and aluminium trichloride are demethylated in an organic solvent to generate 2,4-dihydroxy-5-methoxy benzaldehyde; and (b) under the protection of nitrogen, the 2,4-dihydroxy-5-methoxy benzaldehyde and ethyltriphenylphosphonium acetate undergo a pyroreaction in an organic solvent with high boiling point to generate esculetin-6-methyl ether (the Scopoletin). The preparation method can effectively produce the Scopoletin industrially on a large scale.

The invention relates to a process for the isolation of compound scopoletin which is used as nitric oxide synthesis inhibitor from Artemisia annua and other plant families, said process comprising extraction of dried powdered material of different plant parts with an aqueous acetonitrite solvent in the ratio of 1:5 for 6 to 8 hrs., concentration of the extracted solvent upto 30% of its original extract under vacuum, partitioning the concentrated extract with halogenated solvent to transfer scopoletin in the non-polar halogenated solvent, drying halogenated solvent over anhydrous sodium sulphate and evaporating the solvent, crystallizing the residues in methanol and filtering the crystals, concentrating the filtrate and chromatographed on silica gel, eluting scopoletin in chloroform methanol mixture; and crystallization of the fractions containing the scopoletin to get the pure scopoletin compound.

The invention provides a method for acquiring functional components in morinda citrifolia. The method comprises the following steps: firstly, drying and crushing the morinda citrifolia; secondly, extracting by using water or an organic solvent. The method is characterized in that the method comprises the following steps: (A) in the first step, picking up the white morinda citrifolia; (B) in the first step, a drying method adopts infrared drying; the functional components are total phenols, general flavones, total saponins, scopoletin or aucubin. The invention further provides a morinda citrifolia extract prepared according to the method and application of the morinda citrifolia extract in preparation of health-care products and drugs. According to the method provided by the invention, the extraction efficiency of the total phenols, the general flavones, the total saponins, the scopoletin or the aucubin in the morinda citrifolia can be remarkably improved, and the contents of the functional components in the morinda citrifolia extract are remarkably increased.

The invention relates to a method for extracting scopoletin from erycibe obtusifolia benth, which comprises the following steps of: crushing the erycibe obtusifolia benth serving as a raw material to 40 to 80 meshes, degreasing the material by using petroleum ether, adding 70 percent ethanol into the material to perform ultrasonic extraction for 30 to 60 minutes, concentrating the extracted substance to obtain extract, dissolving the extract in thin alkali, filtering the solution, adding acid into the filtrate for reduction, continuously concentrating the filtrate to obtain acidified extract, adding chloroform into the acidified extract for extraction, reclaiming the chloroform, and re-crystallizing to obtain a product by using 75 to 90 percent ethanol. The method for producing the product has thorough extraction and little pollution and can be used for large-scale production.

The invention provides plant miticide prepared by taking scopoletin lactone as the main raw material and then adding in solvent, solubilizer and / or emulsifier, is environmental-friendly harmonious pesticide which is safety to human beings and animals, and has profound significance in the long-termed development of the ecological environment, the health of human beings and the integrated control of diseases. Compared with traditional pesticide, the scopoletin lactone plant miticide is characterized in and has the advantages that: 1. the scopoletin lactone exists in nature is a large amount, has low toxicity, is easy to degrade and is safety to the ecological environment; 2. the scopoletin lactone extraction method is simpler; 3. the scopoletin lactone can be also synthesized manually, has low cost and is easily applied to industrial large-scale production; 4. the scopoletin lactone can be solved in various solvent, is easy to be mixed with other anti-mite components to prepare medicine, and can play the role of improving the anti-mite effect; and 5. the scopoletin lactone can inhibit cellular signal transduction, is good neuromuscular blocking agent, and can quickly palsies harmful mites.

The invention relates to the fields of organic synthesis and medicinal chemistry, and particularly relates to scopoletin derivatives having anti-tumor activity and shown in a general formula I or II, a preparation method of the scopoletin derivatives, pharmaceutical compositions including the scopoletin derivatives and application of the scopoletin derivatives and the pharmaceutical compositions. Pharmacodynamic experiments prove that the scopoletin derivatives disclosed by the invention have the anti-tumor activity and can be applied to treatment of a breast cancer, a lung cancer, a colorectal cancer, a cancer of the liver, a gastric cancer, neuroglioma, an ovarian cancer, a cervical cancer, a thyroid cancer, a pancreatic cancer or histiocytic lymphoma.

The invention relates to a method for extracting chlorogenic acid and scopoletin from tobacco leaf. The method comprises the following steps: extracting a tobacco leaf raw material with a petroleum ether, filtering, performing evaporating to dryness under reduced pressure so as to obtain extract, extracting with ethyl acetate to obtain an extracting solution, adding water into filter residues andextracting with ethanol, extracting the filtrate obtained by filtration with ethyl acetate, performing vacuum concentration to obtain an extracting solution, mixing the extracting solution, loading onto a silica gel column of 80-100 meshes, respectively eluting with water and ethyl acetate, performing vacuum concentration, crystallizing and recrystallizing so as to obtain the products chlorogenicacid and scopoletin, carbonizing filter residues at a temperature of 150-180 DEG C, and pelletizing to obtain a carbide so as to be directly used as an adsorbent. According to the method disclosed bythe invention, the chlorogenic acid and scopoletin can be synchronously extracted, the utilization rate of the tobacco leaf is effectively improved, and the production cost is reduced. By utilizing water extraction, alcohol extraction and petroleum ether extraction, most of substances in the tobacco leaf are removed, so that the extracted wastes can be used for preparing adsorbing materials, and the yield of the chlorogenic acid and scopoletin is improved.

The invention discloses a scopoletin derivative as well as a preparation method and an application thereof. The general formula of the scopoletin derivative is formula (I), wherein R is selected from C1-4 alkyl and X is selected from a halo substituent. The scopoletin derivative has a remarkable anticoagulation effect and can remarkably prolong the in vitro blood coagulation time. The scopoletin derivative orally taken can quickly enter into blood to have the anticoagulation effect. The onset time and the curative effect of the scopoletin derivative for treating venous thromboembolic diseases are equivalent to those of warfarin and the scopoletin derivative is smaller in dosage and easy to control. The scopoletin derivative has novel medical use of preventing and treating venous thromboembolic diseases and meanwhile provides a novel treatment candidate medicine for patients with venous thromboembolic diseases. The invention further discloses the preparation method of the scopoletin derivative and the application of the scopoletin derivative in preparing anticoagulation medicines.

The invention discloses an analytical method for detecting an organophosphorus pesticide by a ratio-dependent fluorescence sensor based on manganese dioxide nanosheet response. The analytical method comprises the following steps: mixing and reacting the organophosphorus pesticide with acetylcholin esterase to obtain a mixed solution, then adding an acetylcholine chloride solution and a PBS buffersolution into the mixed solution for a reaction so as to obtain a solution, adding a manganese dioxide nanosheet soluktion and the PBS buffer solution into the solution for a reaction at room temperature to obtain a mixture, finally adding Scopoletin and Amplex Red into the mixture for a reaction in a dark environment, and recording a solution fluorescence spectrum to obtain the concentration of the organophosphorus pesticide through fluorescence intensity. Without the need of the detection of expensive precise instruments, the detection method is simplified, the detection cost of the organophosphorus pesticide is greatly reduced, and the analytical method has the advantages of low operation cost, quick, simple and convenient detection, high selectivity and the like.

The invention discloses a medicated leaven thin-layer chromatography detecting method which comprises the following steps: (1) test solution preparation: taking herb powder to be detected, adding methanol for extraction, filtering, concentrating, and adding methanol to be the constant volume to be used as the test solution; (2) reference solution preparation: preparing the reference solution from a scopoletin comparison product or preparing the reference solution from the scopoletin comparison product and a negative control herb respectively; (3) thin-layer detection: conducting sample application on the test solution and the reference solution on a thin-layer plate, developing by using petroleum ether-ethyl acetate-formic acid as a developing solvent, inspecting and comparing, wherein the ratio of petroleum ether to ethyl acetate to formic acid is (5-7):(3.5-4.5):(0.05-0.15). According to the medicated leaven thin-layer chromatography detecting method, various components in the medicated leaven can be effectively separated, the stable and reliable index component scopoletin can be found, the quality of the medicated leaven herb can be accurately and reliably monitored, and effective guarantee is provided for clinical effects.

The invention relates to application of scopoletin in the preparation of a drug for preventing and treating tumor, wherein the drug for preventing and treating the tumor is a nuclear transcription factor-kB inhibitor. The invention also provides a pharmaceutical composition for treating or preventing the tumor, which contains effective amount of curative scopoletin and a medicinal carrier, or drugs for treating or preventing the tumor. The nuclear transcription factor-kB inhibitor is an effective monomer component of natural Chinese medicine, which has small toxic side effect, high safety, and specific function target point, and can be used for preparing medicaments of the nuclear transcription factor-kB inhibitor or antitumor medicaments.

The invention provides a method for extracting scopoletin. The method comprises the following process steps of: performing heating and refluxing extraction on artemisia annua residues from which artemisinin is extracted and which serves as the raw materials by using methanol, acetone, ethyl acetate or dichloromethane under the conditions of temperature of 53 DEG C and vapor pressure of 0.06 MPa, concentrating; separating by column chromatography; and crystallizing and the like. The artemisia annua residues serve as the raw materials, so the method can be in seamless connection with the production line of industrialized extraction of the artemisinin, investment of purchasing the raw materials is saved and the cost of the raw materials is greatly reduced. The method is easy to operate. By the method, the scopoletin can be effectively extracted; and the purity of the scopoletin in the obtained extracts is high.

The invention relates to a detection method for Naoxinqing capsules, belongs to the technical field of traditional Chinese medicines and provides the detection method for the Naoxinqing capsules. Through the method, the quality of the Naoxinqing capsules can be better controlled. The method comprises the following step of carrying out scopoletin detection: using octadecylsilane chemically bonded silica as a filler, using methanol and 0.1 percent formic acid as flowing phases, adopting the detection wavelength of 345nm, taking a right amount of scopoletin reference sample, adding methanol to prepare into a reference solution, taking materials in the Naoxinqing capsules under the content uniformity item, precisely adding a methanol solution, carrying out ultrasonic processing, filtering, taking subsequent filtrate so as to obtain a test solution, respectively and precisely sucking the reference solution and the test solution, injecting the reference solution and the test solution into a liquid chromatograph, carrying out measurement, calculating by a peak area according to an external standard method so as to obtain the content of the scopoletin. The Naoxinqing capsules are detected according to the method, if the content of the scopoletin in each capsule is less than 5[mu]g, the drug is unqualified.

The present invention discloses six-sprout six-bean chocolate, made from the following ingredients: cocoa liquor blocks, cocoa butter, skimmed milk powder, green bean sprouts, Chinese Toon Sprouts, buckwheat sprouts, peanut sprouts, radish sprouts, ginger buds, colored beans, Lens culinaris, Phaseolus coccineus, red kidney beans, chick peas, baiyu bean (a bean mainly produced in Yushan County), trehalose, glycoprotein isolated from Chinese yam, edible salt, beta-sitosterol, ginkgetin, scopoletin and deoxynojirimycin. Six sprouts and six beans are added into the product, the product is nutrient-rich and comprehensive in health care function, can promote children's growth and development, enhance immunity, beautify, protect skin and protect heart and cerebral vessels, is anti-oxidation, anti-aging and anti-tumor, and can help weight losing and body slimming; a variety of active plant ingredients are added and placed in the innermost layer of the chocolate to ensure the effectiveness and durability; the six-sprout six-bean chocolate comprises a chocolate thick layer, a skimmed milk powder layer, a chocolate thin layer and a six-sprout six-bean layer in turn from outside to inside, and the six-sprout six-bean chocolate is clearly layered, harmonious in flavor, diverse in taste and delicate in taste and can give consumers different taste experience.

The invention relates to a method for using coumarin compound to prepare induced nerve stem cell directional differentiation drug. Wherein, said compound can be aesculetin, 7-hydroxycoumarin, megeresin, aesculetin, and scopoletin, etc. The test has proved that it has significant induced nerve stem cell directional differentiation function. And the prepared medicine has coumarin compound as active component, with acceptable carrier, while the active component content is 0.1-99.5%, and the inventive drug can be tablet, injection, etc.

The invention relates to a method for synchronously and rapidly detecting nine functional active components in Yedao deer and turtle wine and belongs to the field of researches of functional components of health-care wine. The method comprises the following steps: carrying out qualitative analysis on the active components in the Yedao deer and turtle wine by adopting a liquid chromatography-mass spectrometry method through a positive ion scanning working mode; then carrying out quantitative analysis by adopting an external standard method. The method provided by the invention has the advantages of simple pre-treatment steps, high precision, low quantification limit and good stability and can be used for rapidly determining the content of Chinese yam sapogenin, 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside, betaine, ligustilide, scopoletin, atractylenolide III, emodin, emodin methyl ether and codonopsis pilosula alkyne glucoside in a Yedao deer and turtle wine sample; the method can be used for synchronously and rapidly determining the components in the Yedao deer and turtle wine, a Chinese herbal medicine mixed extracting solution of a similar formula or prepared wine.

The invention provides application of a radix physochlainae extract in preparation of drugs for preventing and treating myopia. Effective compositions such as anisodamine, scopolamine and scopoletin in the radix physochlainae extract are obtained through tests, the purpose of preventing and treating myopia can be achieved, and the defect that clinical application cannot be achieved due to potential side effects caused by long-term single use of anticholinergic drugs can be effectively overcome. The radix physochlainae extract has a remarkable treatment effect on myopia, is high in safety, andis expected to be popularized and applied clinically. Experiments prove that the radix physochlainae extract can be used for improving myopia by regulating scleral fibrous layers and retinal pigment cells, influencing expression of matrix metalloproteinase 2, matrix metalloproteinase inhibitor 2 and the like, regulating spasm of ciliary muscles and the like. The invention also provides a composition. The composition comprises effective components of the radix physochlainae extract; and the composition provides a new way for preventing and treating myopia.

The invention relates to a quality control method of Chinese patent medicine, in particular to a quality control method of grosvenor momordica compound preparation. The invention can conduct effective quality control on the grosvenor momordica anticough tablet and is also suitable for the capsules, soft capsules and pills prepared according to the prescription. Based on the original standard, the quality control method rectifies the discrimination method of lamina of balloonflower root, which uses the water as extract dissolvent and developer instead of aether, and uses ultrasonic extraction instead of reflux extraction, with more covenient and safe operation as well as specialization; the invention adds the content measuring of scopoletin of cortex mori radicis so as to control the quality of grosvenor momordica and the various medicine forms effectively.

The invention discloses a method for extracting, separating and purifying scopoletin from erycibe schmidtii craib. The method comprises the following steps: (1) grinding erycibe schmidtii craib stemsto obtain erycibe schmidtii craib stem powder; (2) uniformly mixing the erycibe schmidtii craib stem powder and ethanol, performing reflux extraction at the temperature of 50-60 DEG C so as to obtainan extracting solution, wherein a solid-liquid ratio is 1:(5-8)kg / L, and recovering the solvent so as to obtain extract A; (3) adding an acid aqueous solution into the extract A for soaking for threetimes, and separating to obtain a precipitate B; (4) dissolving the precipitate B into 60-70% of ethanol, loading onto a macroporous resin column, eluting with water and different ratios of ethanol water in sequence so as to obtain an eluent, and concentrating to obtain crude scopoletin; and (5) dissolving the crude scopoletin into 80-90% of ethanol, and recrystallizing, thereby obtaining the scopoletin. The raw materials selected by the method are wide in distribution. Moreover, according to the method, the process is simple, the cost is low, the environmental-friendly and high-efficiency effects are achieved, the product yield is high and the method is suitable for industrialized production.

The invention belongs to the synthesis field of isotope labeled compound synthesis, and particularly relates to scopoletin labeled by stable isotope and a synthetic method for the scopoletin. The synthetic method is characterized in that scopoletin labeled acetylchloride-2-13 C is taken as a raw material, and the scopoletin labeled acetylchloride-2-13C reacts with 2,4-dihydroxyl-5-methoxybenzaldehyde to generate 2-aldehyde-4-methoxy-5-hydroxyl phenyl acetate; under the action of a basic catalyst, scopoletin-2-13C is generated through Perkin reaction. The synthetic method is simple in synthesis step, is gentle in reaction condition, is simple and convenient in operation, is high in product yield being 26% or higher, is capable of effectively synthesizing an isotope labeled coumarin derivative, so that a novel way is provided for synthesizing the isotope labeled coumarin derivative. Certain innovation and unique research significance are achieved on organic synthetic method.

The present invention discloses an artemisia carvifolia biotransformation mycelium and an extract and a use thereof. A preparation method of the artemisia carvifolia biotransformation mycelium is as follows: hericium erinaceus is inoculated into a hericium erinaceus culture medium and cultured at 20-27 DEG C for 30-40 d, and mycelium is taken out and dried to obtain the artemisia carvifolia biotransformation mycelium; every 100 parts of the hericium erinaceus culture medium comprises 50-70 parts of a basal medium and 30-50 parts of artemisia carvifolia; the basal medium comprises the followingcomponents in parts by weight: 350-490 parts of wheat bran, 100-140 parts of corn flour, 50-70 parts of bran coat and 400-600 parts of water; the artemisia carvifolia biotransformation mycelium contains fatty acid substances and ketosteroid substances; and the fatty acid substances comprise palmitic acid, linoleic acid, oleic acid and stearic acid and the ketosteroid substances comprise ergost-7,22-diene-3-one, stigmasta-4-ene-6-alpha-ol-3-one, praeruptorin, scopoletin and byakangelicin. The extract of the artemisia carvifolia biotransformation mycelium has an anti-helicobacter pylori effect,has the effect obviously better than that of hericium erinaceus mycelium or artemisia carvifolia, and can be used for preparing an anti-helicobacter pylori drug.

The invention discloses optimization of an extraction process of the throat clearing and nourishing preparation by means of an orthogonal test. The extraction process regards comprehensive weighted score of gallic acid, scopoletin, scoparone, nuciferine content and an extract yield rate in the extract as an evaluation index, adopts an L9 (3<4>) orthogonal test, takes ethanol concentration, a ratioof solid to liquid, a number of extraction times and extraction time as investigation factors, optimizes the extraction process of the throat clearing and nourishing preparation, and carries out process verification. The extraction process of the throat clearing and nourishing preparation optimized by means of the orthogonal test has good feasibility and repeatability, and provides a certain basis for the subsequent production process research.

The invention discloses a medicine composition with effects of relieving cough and removing phlegm. The medicine composition comprises the following components in part by weight: 1-10 parts of glycine betaine, 1-10 parts of scopoletin, 10-40 parts of morusin and 10-20 parts of glycyrrhizic acid. According to experimental pharmacological studies, the medicine composition has pharmacological activities of cough relieving and phlegm removing, and can be used for treating diseases such as cough and pneumonia.

The invention relates to a scopoletin and di-bisdemethoxycurcumin complex suspending agent which is prepared from the following components by mass percent: 1-10% of scopoletin, 1-10% of di-bisdemethoxycurcumin, 2-8% of wetting dispersing agent, 2-8% of thickener, 2-8% of freeze proof agent, 1-5% of defoamer and the balance of water, wherein the scopoletin and dual-bisdemethoxycurcumin are both plant source secondary metabolite, have the characteristics of high efficiency, low toxicity, less residual and the like; and two medicines has certain differences on the modes of acting to Tetranychus cinabarinus Boisdu and target esterase, the characteristics of the two medicines are composited after complexing, and multiple action modes such as touching out, inner absorption, ovicidal action, repellent, oviposition inhibitory activity and the like are provided. Simultaneously, the suspending agent can be prepared into a suspension concentrate, which can reduce the pollution of an organic solvent on the environment, the toxicity of pesticides, the production cost and the like, and can improve the safety.

The invention provides the use of an eurycoma longifolia extract for treating bacterial infection and dermatitis. The eurycoma longifolia extract comprises scopoletin, 9-methoxy-canthium dicoccum-6-ketone, eurylene and the like. Bacteria comprise escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, streptococcus pyogenes, candida albicans, klebsiella pneumoniae and pseudomonas aeruginosa.