27 results about "Scopoletin" patented technology

The application relates to a method for hydrothermal acid-controlled degradation of mulberry leaf polysaccharide and antioxidant application thereof, and belongs to the technical field of extraction of active ingredients in natural products. The method comprises the following steps: pretreating raw materials, hydrothermally reacting and acid-catalytically treating mulberry leaves, and alcohol-sedimenting, separating and purifying mulberry leaf polysaccharide, so as to obtain mulberry leaf polysaccharide, finally, the antioxidant application of the mulberry leaf polysaccharide is explored, and a theoretical basis is provided for development of mulberry leaf resources. The method can not only obtain mulberry leaf polysaccharide, but also obtain high-value natural active ingredients such as rutin, astragalin, scopoletin and isoquercitrin in the mulberry leaves, is beneficial to development and utilization of the mulberry leaf resources, meets the needs of people's health, and has practical significance and economic value.

The invention discloses a prismatomeria tetrandra fingerprint spectrum construction method and application thereof. Belongs to the technical field of medicine. The construction method comprises the following steps: taking scopoletin, coumarin, methyl isoalizarin-1-methyl ether and methyl isoalizarin reference substances, and dissolving the reference substances in a solvent to prepare a reference substance solution; taking a prismatomeris tetrandra sample, adding an extraction solvent for extraction, and filtering an extracting solution to prepare a test solution; respectively carrying out high performance liquid chromatography detection on the reference substance solution and the test solution, and constructing the fingerprint spectrum of the prismatomeria tetrandra. Compared with the prior art, the method has the beneficial effects that the fingerprint spectrum of the prismatomeria tetrandra is established by adopting an HPLC (High Performance Liquid Chromatography) method and is used for identifying and controlling the quality of the prismatomeria tetrandra medicinal material, so that a standard for comprehensively and effectively controlling the quality of the prismatomeria tetrandra medicinal material is expected to be established.

The invention belongs to the technical field of green biological prevention and control of plant diseases, and particularly relates to a biopesticide based on coumarin metabolites and application of the biopesticide in green prevention and control of litchi downy blight. A novel litchi downy blight prevention and control method is developed based on coumarin compounds, and the method passes through in-vitro bacteriostasis experiments, fresh leaf bacteriostasis experiments and comprehensive evaluation of the prevention and control effect. According to the present invention, it is found that the three coumarins such as Scopoletin, Esculin and Esculin can significantly inhibit the growth of peronophythora litchii on a culture medium and a living body leaf, and have good prevention and control effects, and the Scopoletin and Esculin have the most significant antibacterial effect, and can synergistically enhance the inhibition effect on peronophythora litchii, such that the Scopoletin, Esculin and Esculin can significantly inhibit the growth of peronophythora litchii. The method has the advantages of environmental protection, high efficiency, safety and economy, is suitable for large-scale application, and provides a new solution for the prevention and treatment of the litchi downy blight.

The invention provides a method for identifying authenticity of semen armeniacae amarae and peach kernel medicinal materials, decoction pieces and formula granules based on scopoletin components, which adopts a unique double-solvent system developing solvent aiming at the scopoletin components, can quickly and effectively identify the authenticity of the semen armeniacae amarae and peach kernel medicinal materials, decoction pieces and formula granules, and can save the cost and improve the accuracy of identification of the semen armeniacae amarae and peach kernel medicinal materials, decoction pieces and formula granules. The method is high in precision and sensitivity, good in stability, convenient and fast, capable of accurately identifying the peach kernels and the bitter apricot kernels and good in application prospect.

The invention relates to the technical field of biosynthesis, in particular to a microbial synthesis method and application of scopoletin with tobacco leftover as a substrate, the microbial synthesis method of scopoletin with tobacco leftover as the substrate comprises the following steps: S1, transforming recombinant plasmids into different host cells to obtain corresponding engineering strains; and S2, carrying out fermentation culture on the engineering strain, and adding a degradation product of the tobacco residue in the fermentation process to obtain the scopoletin. The invention aims to improve the yield of scopoletin synthesized by microorganisms and reduce the synthesis cost of scopoletin by screening host cells and optimizing recombinant plasmids.

The invention belongs to the technical field of fishery medicines, and relates to specific therapeutic activity of scopoletin, in particular to application of scopoletin in preparation of a medicine for resisting white spot syndrome. Experiments prove that scopoletin can endow aquatic animals such as prawns with certain white spot syndrome virus resistance by inducing up-regulation of key member Ago2 and Dicer2 genes in a prawn RNAi (Ribonucleic Acid Interference) antiviral mechanism.

The invention provides a mugwort glycosyl transferase AarUGT84A120 coding gene, a protein and application, and belongs to the technical field of biology. The method is based on related results of whole genome sequencing of folium artemisiae argyi. The key enzyme AarUGT84A120 in the last step of synthesis of eupatidinin-7-O-glucoside, jaceoside-7-O-glucoside, homoplanid-7-O-glucose, scopoletin-7-O-glucoside, isofraxidine-7-O-glucoside, daphnedin-7-O-glucoside and aesculetin-7-O-glucoside is screened and identified, and the key enzyme AarUGT84A120 is used for the first step of synthesis of eupatidin-7-O-glucoside, jaceoside-7-O-glucoside, homoplanid-7-O-glucoside, homoplanid-7-O-glucoside, homoplanid-7-O-glucoside, the blank of biosynthesis paths of flavonoid glycoside components in the folium artemisiae argyi is filled. In order to further biologically synthesize eupatidinin-7-O-glucoside, jaceosiidin-7-O-glucoside, homoplantaginin-7-O-glucoside, scopoletin-7-O-glucoside, isofraxidine-7-O-glucoside, daphnedin-7-O-glucoside, and the like, the invention also relates to a biosynthesis method of the eupatidinin-7-O-glucoside and the biosynthesis method of the eupatidinin-7-O-glucoside. The aesculetin-7-O-glucoside provides a glycosyl transferase protein and a coding sequence of the glycosyl transferase protein. The method has important significance for understanding the quality formation of the folium artemisiae argyi, and meanwhile, a key element is provided for the biological research on the synthesis of flavonoid components.

The invention discloses application of scopoletin in brown planthopper resistance of rice and a detection method. According to the application of scopoletin in preparation of biopesticides or plant insect-resistant agents for preventing and controlling brown planthoppers, the scopoletin directly acts on the brown planthoppers, and the survival rate, the weight gain, the egg laying amount and the number of yeast-like symbiotic fungi in the brown planthoppers are inhibited. According to the method for screening brown planthopper-resistant rice, the content of scopoletin in leaf sheath tissue of a rice plant is larger than or equal to 80 micrograms per gram of dry weight, and the survival rate of brown planthoppers is reduced by 60% within 7 days in an artificial insect inoculation experiment. According to the invention, direct association between scopoletin and brown planthopper resistance in rice is discovered for the first time, and a new metabolite-mediated insect resistance theory is provided; meanwhile, the composition acts on physiological, reproductive and symbiotic bacteria of pests, so that the drug resistance risk is reduced; the method is environment-friendly, natural in source and low in residue; the application range is wide, and biopesticide and insect-resistant breeding screening indexes can be developed; all the methods are successfully reproduced in a laboratory, and the method has an industrial transformation basis.

The invention aims to provide a method for determining the content of main effective components of liver-protecting Buzufever granules and application, and aims to overcome the defect that the conventional single-component determination method is difficult to comprehensively reflect the overall quality of a preparation and solve the technical problem of lack of accurate quantification of multiple active components at the same time. According to the method, 17 characteristic components such as isopsoralen, scopoletin, aesculetin and the like are simultaneously determined by adopting an ultra-high performance liquid chromatography-tandem mass spectrometry technology and optimizing chromatography and mass spectrometry conditions. According to the technical scheme, higher-throughput and more comprehensive multi-index synchronous quantification is achieved, higher sensitivity and specificity are achieved, linearity is good, precision is high, repeatability and stability meet requirements, and quality evaluation of the complex compound preparation can be accurately and efficiently completed. According to the technical scheme provided by the invention, a more scientific and comprehensive modern quality control standard is established for the liver-protecting Buzufever granules, and the quality controllability and the medication safety guarantee level are remarkably improved.

The invention discloses an application of a tobacco transcription factor NtELF3 in regulation and control of accumulation of polyphenol substances in tobacco leaves. The CDS sequence of the NtELF3 gene is as shown in SEQ ID NO. 1. The amino acid sequence coded by the tobacco transcription factor NtELF3 gene is as shown in SEQ ID NO. 2. According to the invention, an NtELF3 gene sequence in a tobacco 'Honghua Dajinyuan (HD)' variety is cloned; the sequence is overexpressed under the background of Honghua Dajinyuan by utilizing a transgenic technology, so that the contents of total polyphenols and five main polyphenols such as chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, rutin and scopoletin in transgenic line tobacco leaves are remarkably increased. The tobacco NtELF3 gene disclosed by the invention provides a new gene resource and a new breeding thought for improving the tobacco quality through molecular design breeding, and has a good application prospect in the aspect of tobacco polyphenol substance accumulation.

The invention discloses an application of an overexpressed AbMYB93 gene in enhancing the resistance of atropa belladonna to stem rot caused by fusarium, a fusarium strain is separated from a typical atropa belladonna diseased plant, and the fusarium strain can cause stem rot and finally cause death of the plant. Further research finds that the plant hormone brassinolide (BR) can enhance the resistance of the atropa belladonna to the pathogen and increase the TAs content at the same time. Afterwards, the invention reveals that the BR directly up-regulates the expression of a key biosynthetic gene of scopoletin through AbMYB93, so that the resistance of atropa belladonna to the pathogen is enhanced. In conclusion, the research results show that AbMYB93 is a novel regulatory factor for the disease resistance of atropa belladonna, and a practical method for solving the disease resistance of atropa belladonna is established.

The invention discloses a tobacco NtSHN3 gene, an encoding protein and application of the tobacco NtSHN3 gene. It is found for the first time that the transcription factor NtSHN3 can promote synthesis of tobacco chlorogenic acid and inhibit synthesis accumulation of scopoletin. A genetic material of which the content of chlorogenic acid is increased and the content of scopoletin is reduced due to overexpression of the NtSHN3 gene is obtained by utilizing a transgenic technology. A genetic material of which the function of the NtSHN3 gene is weakened, so that the content of chlorogenic acid is reduced and the content of scopoletin is increased is obtained by utilizing an RNAi technology. And a new way is provided for regulating and controlling beneficial metabolites in tobacco planting and obtaining new varieties.

The present invention discloses a esculetin methyltransferase protein from the traditional Chinese medicine Artemisia annua, as well as its encoding gene and application. The protein is the protein described in a) or b) below: a) a protein consisting of the amino acid sequence shown in Sequence 2 in the sequence listing; b) a protein derived from a) in which the amino acid sequence shown in Sequence 2 in the sequence listing is substituted and / or deleted and / or added with one or more amino acid residues and has esculetin methyltransferase activity. The present invention is based on enriching scopoletin from the traditional Chinese medicine Artemisia annua, screening AaOMT1 using transcriptome and proteome, and identifying its ability to catalyze the production of scopoletin through enzymatic activity. Identifying a methyltransferase that directly and specifically produces scopoletin can provide a new pathway for the biosynthesis of scopoletin and a new reference for drug preparation, purification, and synthetic design.

The application discloses a tobacco leaf baking quality improvement microbial agent and application thereof, and the active component of the improvement microbial agent is composed of bacillus amyloliquefaciens and bacillus subtilis, and the improvement microbial agent is sprayed on the surface of tobacco leaves before and after harvesting and before baking. Through application of the specific compound microbial agent, the leaf microorganism community in the tobacco leaf conditioning process can be effectively controlled, and beneficial metabolism can be directionally promoted. Application results show that the application can significantly increase the contents of total sugar, reducing sugar, potassium and chlorine of tobacco leaves, selectively increase polyphenol substances such as scopoletin and rutin, enrich aroma components such as linalool and spirorolandane which give woody and floral styles, and improve the softness of tobacco leaves, so that the characteristic quality of honey sweet type tobacco leaves is comprehensively improved.

The present application belongs to the technical field of fishery drugs, and relates to specific therapeutic activity of scopoletin, and particularly to application of scopoletin in preparation of drugs against white spot syndrome. The present application verifies through experiments that scopoletin can up-regulate Ago2 and Dicer2 genes which are key members in an anti-virus mechanism of RNAi of prawns, and can endow prawns and other aquatic animals with certain resistance to white spot syndrome virus.

The invention discloses a BPNs chemiluminescence probe, a BPNs chemiluminescence probe loaded scopoletin composite material and a chemiluminescence detection method of the BPNs chemiluminescence probe loaded scopoletin composite material. The BPNs chemical light-emitting probe synthesized through water bath ultrasonic wave loads scopoletin through hydrogen bond interaction to obtain the composite material, and the composite material has efficient active oxygen scavenging capacity and reacts with potassium permanganate to generate a strong chemical light-emitting signal. On the basis, a chemiluminescence detection platform of the BPNs / scopoletin is constructed. The method has the advantages of high sensitivity, low detection cost, simplicity and convenience in operation and the like.

The application provides a feruloyl-CoA 6'-hydroxylase pocket mutant and application. The feruloyl-CoA 6'-hydroxylase pocket mutant is obtained by amino acid mutation of the amino acid sequence shown in SEQ ID NO. 1, and includes at least one of the following amino acid mutations: L141N, L141G, A142N, Q144D, F156Y, V157T, N311G. The application also provides an engineering bacterium for synthesizing coumarin compounds by using the feruloyl-CoA 6'-hydroxylase mutant. The feruloyl-CoA 6'-hydroxylase mutant has good enzyme activity and stability, so that the engineering bacterium can not only realize efficient synthesis of coumarin compounds such as scopoletin, aesculin, aesculin glycoside and anisodamine, but also realize efficient de novo biosynthesis of the coumarin compounds, thereby providing a reference for microbial synthesis of the coumarin compounds.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a method for extracting scopoletin from artemisia apiacea residues. The method for extracting scopoletin from the artemisia apiacea residues comprises the following steps: S1, carrying out heating reflux extraction on the artemisia apiacea residues, and carrying out suction filtration while the artemisia apiacea residues are hot to obtain filtrate; s2, concentrating the filtrate obtained in the step S1 into extract; s3, dissolving the extract obtained in the step S2 with methanol, diluting with water to obtain an extract suspension, and enriching with macroporous adsorption resin to obtain an artemisia apiacea residue extract; and S4, recrystallizing the artemisia apiacea residue extract obtained in the step S3, and obtaining the high-purity scopoletin by adopting a one-step method. The invention provides a new thought for further mining the utilization value of the artemisia apiacea residues and improving the utilization rate of wastes, and also provides a raw material guarantee for the development of the scopoletin immune resistance inducer.

The invention discloses a tobacco NtWIN1 gene, an encoding protein and application of the tobacco NtWIN1 gene. It is found for the first time that the transcription factor NtWIN1 can promote synthesis of tobacco chlorogenic acid and inhibit synthesis accumulation of scopoletin. A genetic material of which the content of chlorogenic acid is increased and the content of scopoletin is reduced due to overexpression of the NtWIN1 gene is obtained by utilizing a transgenic technology. A genetic material of which the function of the NtWIN1 gene is weakened, so that the content of chlorogenic acid is reduced and the content of scopoletin is increased is obtained by utilizing an RNAi technology. And a new way is provided for regulating and controlling beneficial metabolites in tobacco planting and obtaining new varieties.

The invention discloses a scopoletin surface imprinted polymer as well as a preparation method and application thereof, and belongs to the technical field of analysis and detection materials. A metal organic framework MIL-96 is used as a carrier and is combined with a molecular imprinting technology, an MIL-96-coated MIPs surface molecularly imprinted polymer is synthesized, a solid-phase extraction column is prepared, the lowest detection limit of scopoletin is 12.14 ng mL <-1 >, and the lowest quantitation limit of scopoletin is 40.46 ng mL <-1 >. The method for detecting the polyphenols in the tobacco extracting solution is established by combining the high performance liquid chromatography to detect the polyphenols, and the adding standard recovery rate is in a range of 95-102.6%. The method is simple to operate, good in reproducibility and low in cost.

The invention relates to the field of rice blast prevention and treatment, in particular to application of a natural product scopoletin in rice blast prevention and treatment. No scopoletin literature report exists at present, and the scopoletin can be used for preventing and treating rice blast. The technical problem to be solved by the invention is to provide the application of the natural product scopoletin in preventing and treating rice blast. Experiments prove that the effect of the natural product scopoletin on the aspect of inhibiting the magnaporthe oryzae, the vegetative growth of the magnaporthe oryzae is obviously inhibited along with the increase of the concentration, and the pathogenicity of the magnaporthe oryzae is almost completely lost under the interference of the scopoletin with a certain concentration. Therefore, the natural product scopoletin can be used as a novel antifungal agent for preventing and treating rice blast.

The invention provides a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method for simultaneously determining seven chemical components in cortex fraxini. The seven chemical components are aesculin, aesculetin, scopolin, scopoletin, fraxin, fraxetin and fraxetin. The method comprises the following steps: S1, preparation of a sample: S11, preparation of a reference substance solution; s12, preparing a test solution; s2, detecting by adopting a UPLC-MS / MS method, wherein the analysis time of the determination method is short; detection limit and quantitation limit are low, and sensitivity is high; the method can effectively eliminate the interference of complex matrixes of traditional Chinese medicines on the measured components, is high in accuracy, and provides a solid foundation for the research and development of the pharmacological action of coumarin compounds in the cortex fraxini and the comprehensive utilization of the cortex fraxini.

The present invention provides chimeric genes and constructs which can be used to alter the lignin composition of woody plants and increase the saccharification and pulping efficiency of said plants. Particularly the lignin composition is modified by the incorporation of scopoletin.

The invention provides an antimalarial composition. The composition is prepared from the following raw material medicines: artemisinin or a derivative thereof, artemisinin B, arteannuic acid and scopoletin. The composition is used for treating malaria, and the anti-malarial effect of the composition is obviously better than that of artemisinin which is independently used.

A thin-layer identification method for Folium Artemisia argyi and vinegar Folium Artemisia argyi formula granules, comprising: pre-treating Folium Artemisia argyi formula granules to obtain a test solution; pre-treating vinegar Folium Artemisia argyi formula granules to obtain a vinegar Folium Artemisia argyi formula granule solution; taking a scopoletin reference substance and adding methanol for dissolution to obtain a reference solution; performing thin-layer chromatographic testing on the test solution, the vinegar Folium Artemisia argyi formula granule solution, and the reference solution, a thin-layer plate being a silica gel G thin-layer plate, and a developer being n-hexane-ethyl acetate-toluene; placing the plate under an ultraviolet lamp for inspection, if the test solution and the reference solution have the same color fluorescent spots at the same Rf, and the inspected substance also has fluorescent spots at Rf=0.625-0.741, then the inspected substance is Folium Artemisia argyi formula granules; if there are no fluorescent spots, then the inspected substance is vinegar Folium Artemisiae argyi formula granules.

The invention relates to a compound radix zanthoxyli composition effective part extract and a preparation method thereof. Comprising the following components: glycine betaine, DL-arginine, choline, 3-hydroxy-2-methylpyridine, D-(+)-Pro, adenine, guanine, sophocarpidine, Pro-Lo, L-phenylalanine, oxymatrine, kojic acid, fraxetin, Cyclo (leucyl pro), ethyl gallate, scopoletin, daidzin, genistein, hesperidin, Quercetin-3beta-D-glucoside and ellagic acid. The preparation method comprises the following steps: preparing the glycine betaine; the total content of the components is 50%-70% of the effective part extract.

The present invention discloses the use of an oxyglycosyltransferase encoded by the PpUGT22 gene of Peucedanum peucedanum in the biosynthesis of scopoletin, belonging to the field of biotechnology. The nucleotide sequence of the PpUGT22 gene encodes a nucleotide sequence having an amino acid sequence as shown in SEQ ID NO. 2. Scopoletin is obtained by an in vitro enzymatic biosynthesis method using the PpUGT22 protein, providing a new method for synthesizing scopoletin.