9 results about "Scopoletin" patented technology

The application relates to a method for hydrothermal acid-controlled degradation of mulberry leaf polysaccharide and antioxidant application thereof, and belongs to the technical field of extraction of active ingredients in natural products. The method comprises the following steps: pretreating raw materials, hydrothermally reacting and acid-catalytically treating mulberry leaves, and alcohol-sedimenting, separating and purifying mulberry leaf polysaccharide, so as to obtain mulberry leaf polysaccharide, finally, the antioxidant application of the mulberry leaf polysaccharide is explored, and a theoretical basis is provided for development of mulberry leaf resources. The method can not only obtain mulberry leaf polysaccharide, but also obtain high-value natural active ingredients such as rutin, astragalin, scopoletin and isoquercitrin in the mulberry leaves, is beneficial to development and utilization of the mulberry leaf resources, meets the needs of people's health, and has practical significance and economic value.

The invention belongs to the technical field of fishery medicines, and relates to specific therapeutic activity of scopoletin, in particular to application of scopoletin in preparation of a medicine for resisting white spot syndrome. Experiments prove that scopoletin can endow aquatic animals such as prawns with certain white spot syndrome virus resistance by inducing up-regulation of key member Ago2 and Dicer2 genes in a prawn RNAi (Ribonucleic Acid Interference) antiviral mechanism.

The invention discloses application of scopoletin in brown planthopper resistance of rice and a detection method. According to the application of scopoletin in preparation of biopesticides or plant insect-resistant agents for preventing and controlling brown planthoppers, the scopoletin directly acts on the brown planthoppers, and the survival rate, the weight gain, the egg laying amount and the number of yeast-like symbiotic fungi in the brown planthoppers are inhibited. According to the method for screening brown planthopper-resistant rice, the content of scopoletin in leaf sheath tissue of a rice plant is larger than or equal to 80 micrograms per gram of dry weight, and the survival rate of brown planthoppers is reduced by 60% within 7 days in an artificial insect inoculation experiment. According to the invention, direct association between scopoletin and brown planthopper resistance in rice is discovered for the first time, and a new metabolite-mediated insect resistance theory is provided; meanwhile, the composition acts on physiological, reproductive and symbiotic bacteria of pests, so that the drug resistance risk is reduced; the method is environment-friendly, natural in source and low in residue; the application range is wide, and biopesticide and insect-resistant breeding screening indexes can be developed; all the methods are successfully reproduced in a laboratory, and the method has an industrial transformation basis.

The invention aims to provide a method for determining the content of main effective components of liver-protecting Buzufever granules and application, and aims to overcome the defect that the conventional single-component determination method is difficult to comprehensively reflect the overall quality of a preparation and solve the technical problem of lack of accurate quantification of multiple active components at the same time. According to the method, 17 characteristic components such as isopsoralen, scopoletin, aesculetin and the like are simultaneously determined by adopting an ultra-high performance liquid chromatography-tandem mass spectrometry technology and optimizing chromatography and mass spectrometry conditions. According to the technical scheme, higher-throughput and more comprehensive multi-index synchronous quantification is achieved, higher sensitivity and specificity are achieved, linearity is good, precision is high, repeatability and stability meet requirements, and quality evaluation of the complex compound preparation can be accurately and efficiently completed. According to the technical scheme provided by the invention, a more scientific and comprehensive modern quality control standard is established for the liver-protecting Buzufever granules, and the quality controllability and the medication safety guarantee level are remarkably improved.

The application discloses a tobacco leaf baking quality improvement microbial agent and application thereof, and the active component of the improvement microbial agent is composed of bacillus amyloliquefaciens and bacillus subtilis, and the improvement microbial agent is sprayed on the surface of tobacco leaves before and after harvesting and before baking. Through application of the specific compound microbial agent, the leaf microorganism community in the tobacco leaf conditioning process can be effectively controlled, and beneficial metabolism can be directionally promoted. Application results show that the application can significantly increase the contents of total sugar, reducing sugar, potassium and chlorine of tobacco leaves, selectively increase polyphenol substances such as scopoletin and rutin, enrich aroma components such as linalool and spirorolandane which give woody and floral styles, and improve the softness of tobacco leaves, so that the characteristic quality of honey sweet type tobacco leaves is comprehensively improved.

The present application belongs to the technical field of fishery drugs, and relates to specific therapeutic activity of scopoletin, and particularly to application of scopoletin in preparation of drugs against white spot syndrome. The present application verifies through experiments that scopoletin can up-regulate Ago2 and Dicer2 genes which are key members in an anti-virus mechanism of RNAi of prawns, and can endow prawns and other aquatic animals with certain resistance to white spot syndrome virus.

The application provides a feruloyl-CoA 6'-hydroxylase pocket mutant and application. The feruloyl-CoA 6'-hydroxylase pocket mutant is obtained by amino acid mutation of the amino acid sequence shown in SEQ ID NO. 1, and includes at least one of the following amino acid mutations: L141N, L141G, A142N, Q144D, F156Y, V157T, N311G. The application also provides an engineering bacterium for synthesizing coumarin compounds by using the feruloyl-CoA 6'-hydroxylase mutant. The feruloyl-CoA 6'-hydroxylase mutant has good enzyme activity and stability, so that the engineering bacterium can not only realize efficient synthesis of coumarin compounds such as scopoletin, aesculin, aesculin glycoside and anisodamine, but also realize efficient de novo biosynthesis of the coumarin compounds, thereby providing a reference for microbial synthesis of the coumarin compounds.

The invention relates to the field of rice blast prevention and treatment, in particular to application of a natural product scopoletin in rice blast prevention and treatment. No scopoletin literature report exists at present, and the scopoletin can be used for preventing and treating rice blast. The technical problem to be solved by the invention is to provide the application of the natural product scopoletin in preventing and treating rice blast. Experiments prove that the effect of the natural product scopoletin on the aspect of inhibiting the magnaporthe oryzae, the vegetative growth of the magnaporthe oryzae is obviously inhibited along with the increase of the concentration, and the pathogenicity of the magnaporthe oryzae is almost completely lost under the interference of the scopoletin with a certain concentration. Therefore, the natural product scopoletin can be used as a novel antifungal agent for preventing and treating rice blast.

The invention provides an antimalarial composition. The composition is prepared from the following raw material medicines: artemisinin or a derivative thereof, artemisinin B, arteannuic acid and scopoletin. The composition is used for treating malaria, and the anti-malarial effect of the composition is obviously better than that of artemisinin which is independently used.