71 results about "Symbiotic bacteria" patented technology

The invention discloses a co-fermentation method based on sphingomonas and a rhodiola rosea extracting solution and application of a product, and belongs to the technical field of microorganisms. By utilizing a special rich enzyme system of sphingomonas and combining with an early-stage composite enzymolysis process and synergistic effect, the wall breaking efficiency of rhodiola rosea cell walls and the conversion degree of active components of rhodiola rosea are greatly improved, the bottlenecks of low efficiency and low bioavailability of a traditional rhodiola rosea extraction method are successfully overcome, and the rhodiola rosea extraction method is suitable for industrial production. The functional components such as salidroside and flavone can be fully released and converted. Secondly, different from non-symbiotic bacteria (such as lactic acid bacteria and saccharomycetes) which are commonly used for fermentation in the prior art, a potential symbiotic bacterium of sphingomonas XJL-TP07 from a special environment is innovatively selected and has better metabolic suitability with the rhodiola rosea component, the fermentation process is milder and safer (no irritant is produced), and the rhodiola rosea fermentation method is suitable for large-scale production. And a synergistic interaction effect can be generated through a unique biotransformation path.

The invention discloses a medicine composition for treating atopic dermatitis, a preparation method and application, the medicine composition comprises inactivated malassezia sphaeroides, and the strain preservation number of the malassezia sphaeroides is ATCC 96807. The cream composition disclosed by the invention is externally applied to local treatment of skin lesions of specific dermatitis, supplements skin symbiotic bacteria, assists in recovering skin barriers of patients, remarkably inhibits growth and proliferation of staphylococcus aureus at the skin lesions of dermatitis and exocrine toxins, remarkably relieves inflammatory response, reduces the thickness of cuticles and reduces inflammatory cell infiltration; the traditional Chinese medicine composition assists the treatment of atopic dermatitis through the effects of the proinflammatory factors and the expression and secretion of the proinflammatory factors, has a remarkable treatment effect on the atopic dermatitis, is quick to take effect, small in toxic and side effects and good in safety, and has a good medicinal prospect.

Pharmaceutical compositions and preparation including outer membrane vesicles (OMVs) and at least one pharmaceutically acceptable carrier wherein said OMVs are native OMVs produced by the Gram-negative commensal bacteria Bacteroides.

The present invention belongs to the field of insect cell culture technology and discloses a method for preparing an Sf9 cell feeder layer. The method mainly comprises two steps: step S1 is subculture of Sf9 cells, in which Sf9 cells are cultured in a suitable culture medium to a suitable cell confluence; step S2 is mitomycin C treatment of the Sf9 cells, in which a specific concentration of mitomycin C is used to treat the subcultured Sf9 cells at a specific time and temperature to inhibit their cell division ability, followed by washing to remove mitomycin C and replacing with fresh culture medium. The feeder layer cells obtained by the present invention can effectively inhibit their own proliferation while maintaining good cell activity and structural integrity. They can survive stably and provide necessary support for the co-culture system. They can be used for the cultivation of insect symbiotic bacteria and other difficult-to-culture insect cells. The method is simple to operate, has mild conditions, and good reproducibility. The prepared feeder layer has a stable effect, providing a powerful tool for related research fields.

The mutant provided by the present invention has an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2, and a gene sequence as shown in SEQ ID NO: 3 or SEQ ID NO: 4. The mutant is obtained by multi-site directed mutagenesis of the wild-type tryptophan 5-hydroxylase of the sponge symbiotic bacteria. The relative enzyme activity of the mutant is up to 206% of that of the wild type. In the test of catalyzing tryptophan to produce 5-HTP, the 10-min tryptophan conversion rate of the mutant can reach 49.82%, which is 2.6 times that of the wild type. The tryptophan 5-hydroxylase mutant provided by the present invention significantly improves the enzyme activity and can be fully used for industrial enzyme catalysis and efficient synthesis of 5-HTP.

The present application relates to the field of microorganism, specifically to three strains of Acrocalymma sp. symbiotic bacteria E00677, E01299A and E01299B and their use for tomato growth. The strains of the present application can promote tomato growth, improve tomato leaf quality, and enhance the antioxidant and stress resistance of tomato.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

The invention discloses an intestinal symbiotic bacterium for enhancing baculovirus resistance of spodoptera frugiperda and application thereof, and relates to the technical field of microorganisms, the intestinal symbiotic bacterium is derived from the midgut of the spodoptera frugiperda, is separated and purified and is subjected to 16S rDNA (ribosomal deoxyribonucleic acid) gene sequencing identification, the sequence length is 1397 bp, and the sequence homology with the known Glutamicibacter soli is 99.93%. The strain is preserved in Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No.34972. The invention provides intestinal symbiotic bacteria for enhancing baculovirus resistance of spodoptera frugiperda and application of the intestinal symbiotic bacteria, and provides an experimental basis for further revealing an interaction mechanism among a host, a pathogen and the symbiotic bacteria. And meanwhile, a technical basis is provided for realizing a comprehensive virus prevention and control strategy of targeted biological prevention and control and non-target beneficial insect protection of the lepidoptera pests.

This invention provides the application of Lycium barbarum polysaccharide (LBP) in the preparation of drugs for the prevention or treatment of intestinal flora and metabolic disorders. Research results show that LBP can significantly improve cisplatin-induced intestinal microbiota and metabolic disorders in rats, manifested by a significant reduction in CDDP-induced serum AST and ALT levels, and improvement in the degree of liver tissue lesions. Furthermore, LBP did not exhibit toxicity in rats. LBP enhanced the diversity and richness of the gut microbiota, restored the structure of dominant symbiotic bacteria, inhibited the proliferation of potentially pathogenic bacteria, and promoted the enrichment of functional bacteria related to SCFAs generation. LBP also restored abnormal metabolism in branched-chain amino acid and aromatic amino acid metabolism, one-carbon metabolism, and lipid metabolism. This invention provides a theoretical basis for the application of natural active polysaccharides in the prevention and treatment of intestinal microbiota and metabolic disorders.

The invention discloses a sugar-inducible promoter and application thereof in prevention and treatment of monochamus alternatus, and relates to the technical field of genetic engineering and biological prevention and treatment. Based on the technical background of transgenic symbiotic bacteria in prevention and treatment of monochamus alternatus, the scheme has the advantages that the transgenic symbiotic bacteria and wild strains have a competitive relationship in ecological niche; under high-level expression driven by a constitutive promoter, the transgenic symbiotic bacteria may be too greedy in resource utilization, so that the transgenic symbiotic bacteria conflict with wild strains and even are eliminated by the wild strains; according to the scheme, expression of Cry3Aa-T protein is regulated and controlled by using the sugar-induced promoter so as to reduce the cost of fitness of exogenous genes carried by serratia marcescens, and a hidden and effective method is provided for preventing and controlling propagation of vector insect monochamus alternatus.

The invention relates to the technical field of biology, in particular to application of osteopontin in regulation of intestinal anaerobic symbiotic bacteria colonization. The osteopontin is used for preparing food or medicine for improving the immunity of the intestinal tract, and the food or medicine is used for improving the abundance of anaerobic symbiotic bacteria in the intestinal tract; according to the application disclosed by the invention, firstly, by virtue of transcriptome analysis, it is proved that the abundance of Parabacteroids distans and Blautiapseudococcoids can be remarkably improved by virtue of the exogenous osteopontin, so that the abundance of the Parabacteroids distans and the abundance of the Blautiapseudococcoids can be remarkably improved; secondly, the invention also proves that the exogenous osteopontin can be used for inhibiting the proliferation of harmful bacteria Escherichia coli; therefore, the intestinal immunity is improved, the intestinal barrier is improved or repaired, and the intestinal flora balance is maintained. The composition is suitable for infants, old people, patients and other people with low immunity.

The present invention discloses primer pairs, a kit, and their applications for identifying three dominant symbiotic bacteria of corn aphids. By designing primers for the three dominant symbiotic bacteria, Acinetobacter soli, Sphingomonas paucimobilis, and Acidovorax wautersii, a multiplex PCR reaction system was constructed for simultaneous detection of the three dominant symbiotic bacteria. This detection method can simultaneously identify the infection status of the three dominant symbiotic bacteria in corn aphid samples using a single PCR reaction system, saving time, reducing costs, and conserving precious experimental samples.

The invention provides an open type tissue culture method of bamboo reed, which comprises the following tissue culture steps: S1, preparation of a gradient antibacterial culture medium: (1) preparation of the gradient antibacterial culture medium: an MS culture medium, 0.5 mg / L 6-BA and 0.1 mg / L NAA are added, 0.1% of tea polyphenol aqueous extract is added, and 0.03% of water-soluble chitosan oligosaccharide is added. Traditional chemical disinfection is replaced with a natural gradient antibacterial system, the dual functions of pollution prevention and control and root protection are achieved by combining a symbiotic bacterium slow release technology, and the stress resistance and lignification characteristics of plants are synchronously improved through the synergistic effect of salinity gradient stimulation and environmental signal molecules; a light-gas intelligent regulation and control system is developed to simulate the optimal growth condition, and a transplanting survival guarantee system is constructed in cooperation with a mycorrhiza pre-activation technology; the dependence of traditional tissue culture on a sterile environment is broken through, and the seedling culture efficiency, the ecological compatibility and the resource utilization level are comprehensively improved under an open condition.

The invention belongs to the technical field of orchid seedling culture, and discloses orchid seed matrix particles for field direct seeding and a preparation method, and the matrix particles comprise coco coir powder, peat soil, a porous inorganic matrix, a degradable adhesive, a bio-organic fertilizer, symbiotic bacteria, orchid seeds and water. The matrix particles adopt a core-shell structure, the core contains seeds, symbiotic bacteria and the like for water retention and moisture retention, and the shell is a porous inorganic matrix and the like for guaranteeing ventilation and mechanical strength. The granules can be directly sown in a field, do not need laboratory seedling culture, can realize high-efficiency seed germination and in-situ mycorrhiza colonization, has the seedling rate close to that of traditional sterile seedling transplantation, reduces labor and facility investment, reduces the disease risk, and is suitable for large-scale planting of orchid plants such as bletilla striata and dendrobe.

The invention relates to an armillaria mellea rhizomorph generation and biomass increasing method based on plant hormone regulation and control, the growth promoting effect of low-concentration 0.1 mg / LZR and / or ET on armillaria mellea is determined for the first time, the traditional cognition that high-concentration hormone inhibits growth is broken through, and the problem that in the prior art, the hormone concentration is difficult to control is solved. The combined use of the ZR and the ET can synchronously optimize the diameter, the number of branches and the biomass of the chords, the comprehensive effect is superior to that of a single hormone, and a new strategy is provided for efficient culture of the armillaria mellea. The method is suitable for liquid fermentation and solid culture, the culture period is short (15-16 days), the production cost of gastrodia elata symbiotic bacteria and medicinal mycelia can be remarkably reduced, and large-scale popularization is easy.

The invention relates to the technical field of secondary metabolite retrieval, and discloses a microorganism secondary metabolite retrieval method based on a knowledge graph, the method is applied to retrieval of alternative items of symbiotic bacteria of valuable and rare medicinal plants, and the method comprises the steps that a retrieval knowledge graph is constructed, key features serve as input, and microorganisms corresponding to the key features are output; performing feature transformation processing to obtain potential substitutes of the key features as to-be-selected transformation features; and based on the to-be-selected transformation features, performing retrieval in the retrieval knowledge graph at least based on the similarity and the attention weight, and outputting alternative items of microorganisms corresponding to the target secondary metabolite. According to the application, dependence on symbiotic bacteria of rare medicinal plants is eliminated, adaptive microorganism substitutes of target secondary metabolites are quickly locked, and the research period is greatly shortened; the screening range of microbial substitutes is widened, the missing of effective strains is reduced, and sufficient strain resource support is provided for large-scale production of medicinal active compounds.

A strain of Dubois nystomatitis ( Dubosiella newyorkensis TZF-002 and its application in the fermentation and conversion of rare ginsenosides belong to the field of microbiology. The *Duboscirella nymphaeus* mentioned herein (…) Dubosiella newyorkensis TZF-002 was deposited at the China Center for Type Culture Collection on December 15, 2025, with accession number CCTCC NO: M 20252890. This invention uses total ginsenosides as raw material, first utilizing the high-β-glucosidase-producing *Duboscirella nystatin* (…). Dubosiella newyorkensis The TZF-002 method employs a mild and controlled fermentation process to transform primary ginsenosides, followed by high-temperature steam treatment, ultimately yielding rare ginsenosides Rg6, F4, Rh4, S-Rg3, R-Rg3, Rk1, and Rg5 efficiently. This method, for the first time, combines the enzymatic transformation characteristics of intestinal symbiotic bacteria with physical treatment, offering advantages such as mild conditions, low cost, and significantly superior product variety and content compared to single processes. It effectively improves the yield, bioavailability, and physiological activity of rare saponins.

The present application relates to biopharmaceutical manufacturing, more particularly to the field of penetration enhancers, and specifically discloses a transdermal absorption penetration enhancer, a preparation method thereof and application in cosmetics. The penetration enhancer comprises plant exosomes, natural penetration activity molecules, film forming agents, ZIF-90 nanomaterials, temperature / ROS dual response ion channel regulating molecules and skin symbiotic bacteria activating materials. The preparation method comprises the steps of plant exosome extraction, hydrophobic / hydrophilic active molecule encapsulation, ZIF-90 nanoshell growth, ion channel regulating molecule anchoring, introduction of skin symbiotic bacteria activating materials, addition of film forming agents, ultrafiltration centrifugal concentration and sterilization filling and the like. Through the triple synergy of plant exosome-ZIF-90 nanocore-shell structure, temperature / ROS dual response regulation and skin symbiotic bacteria activation, the present application realizes efficient, low irritation and intelligent controllable transdermal delivery of active substances, and is suitable for various cosmetic dosage forms.

The application discloses a kind of complex small molecule coral food and its application in adjusting coral symbiotic microbial community structure.The raw materials of the complex small molecule coral food include: soybean meal small peptide protein, cod small peptide protein, haematococcus pluvialis powder, complex amino acid, squid meal, chitosan oligosaccharide and complex nucleotide.The complex small molecule coral food of the application takes soybean meal small peptide protein and cod small peptide protein as main component, haematococcus pluvialis as secondary component, and is supplemented with complex amino acid and squid meal, and is additionally added with chitosan oligosaccharide and complex nucleotide.The obtained complex small molecule coral food not only has good palatability, but also ensures the feeding amount of coral.It is verified by test that the complex small molecule coral food of the application can adjust the symbiotic microbial community structure of aquarium coral, improve the proportion of coral beneficial symbiotic bacteria, and further improve the environmental tolerance of coral.

The invention discloses a microecological preparation capable of regulating and controlling abundance of intestinal bifidobacteria in a targeted mode and application of the microecological preparation, the microecological preparation is composed of intestinal symbiotic bacteria and prebiotics, the intestinal symbiotic bacteria are bacteroides, and the prebiotics are polysaccharide containing N-acetylglucosamine. The prebiotics and the bacteroides multiforme CCFM1441 are combined for use, the prebiotics N-acetylglucosamine can be effectively released, nutrition is provided for bifidobacterium longum, and proliferation of the bifidobacterium longum is promoted. Further, in vitro and in vivo experiments prove that there is a strong cross-feeding activity between Bacteroides multiforme CCFM1441 and Bifidobacterium longum based on prebiotics. The beneficial microecological preparation can promote proliferation of bifidobacterium longum in intestinal tracts in a targeted manner, improves intestinal flora imbalance caused by antibiotics, and further has a huge application prospect in preparation of products (such as food, drugs or health care products) for improving intestinal health.

The invention provides a microbial carrier vaccine, a preparation method thereof and a vaccine composition. A microbial carrier vaccine for antigen delivery with an enteric microbial delivery system is provided, and the enteric microbial delivery system is selected from at least one of a bacteriophage delivery system (e.g., Klebsiella pneumoniae bacteriophage), an Escherichia coli delivery system (e.g., Klebsiella pneumoniae bacteriophage), and an antigen delivery system (e.g., Klebsiella pneumoniae bacteriophage). Escherichia coli S17-1 lambda pi) or a conjugation delivery system of Escherichia coli and intestinal symbiotic bacteria. The microbial carrier vaccine is a vaccine platform developed based on intestinal microorganisms, after the vaccine enters a body, intestinal symbiotic bacteria can be subjected to in-situ editing, so that the intestinal symbiotic bacteria can continuously generate antigens to be applied to the body, the body can be continuously stimulated to generate antibodies, and the continuous protection effect is achieved.

The present application relates to a kind of nanometer preparation based on plant extract delivery antibiotic and its application, belong to nanometer preparation technical field.To solve the problem of low bioavailability and low efficiency in the treatment of pulmonary diseases by oral or intravenous injection of antibiotic, the present application provides a kind of nanometer preparation based on plant extract delivery antibiotic.The mass-volume ratio of antibiotic and plant extract in nanometer preparation is 1-10 mg:20-100 μL.The nanometer preparation of the present application can significantly inhibit the migration of fibroblasts, has obvious bacteriostatic effect, and can reduce the microbial density in lung;Its positive charge is combined with the negative charge on the surface of bacterial cell membrane, affects the transport of transmembrane substances on bacterial cell membrane, affects bacterial metabolism and reproduction, better realizes the regulation of lung symbiotic bacteria, and can alleviate the progression of pulmonary fibrosis.The nanometer preparation of the present application has the advantages of small toxic and side effects, good patient compliance, high encapsulation rate and the like.

The invention belongs to the technical field of microbial genetic engineering and protein delivery, and particularly relates to an engineered bacterial secretion system for efficiently delivering protein and application of the engineered bacterial secretion system. Specifically, on the basis of analyzing a delivery mechanism of bacteroides fragilis GS086 type double-effect protein T6SS, engineering modification is performed on a toxic structural domain of the effect protein, and a bacterial secretion system capable of efficiently and simultaneously delivering a plurality of proteins is constructed. According to the system, a modified strain is a natural human intestinal symbiotic bacterium, the problems of high immunogenicity, poor stability and the like are effectively avoided, and protein can be delivered in situ in the intestinal tract to realize precise regulation and control of an intestinal flora structure and secretion of specific target protein, so that the system has a good practical application value.

This invention relates to an antitumor agent based on respiratory tract symbiotic bacteria, its preparation method, and its application. Addressing the current clinical issue of the unclear role of respiratory flora in lung cancer patients' progression, we provide the first evidence that the respiratory tract symbiotic *Streptococcus osticulata* possesses antitumor activity. When *Streptococcus osticulata* is delivered back into the respiratory tract, it can stimulate an antitumor immune response, and its efficacy can be further enhanced by adenosylcobalamin. Currently, there is a lack of non-invasive, non-invasive inhaled treatments for lung cancer. The antitumor agent of this invention can be combined with novel non-invasive inhaled bacterial-metabolite immunotherapy to achieve a long-term chronic disease management model, representing a safe and transferable treatment approach. In addition to achieving effective lung tumor control when used alone, this therapy can synergistically enhance the efficacy of immunotherapy, inducing complete tumor regression in 50% of tumor-bearing mice.

This invention discloses the application of an artemisia annua compound as the sole active ingredient in the preparation of topical formulations for the prevention or improvement of blackheads and acne. Based on 100% of the total mass of the topical formulation, the artemisia annua compound accounts for 0.1% to 0.22%, and is composed of the following raw materials in parts by mass: camphene 4 to 10 parts, artemisinin 1 part. This invention also discloses a topical formulation for the prevention or improvement of blackheads and acne, the sole active ingredient of which is an artemisia annua compound. Based on 100% of the total mass of the topical formulation, the artemisia annua compound accounts for 0.1% to 0.22%, and is composed of the following raw materials in parts by mass: camphene 4 to 10 parts, artemisinin 1 part. The artemisia annua compound of this invention can significantly reduce the formation of squalene peroxide in a UVA-induced squalene photo-oxidation model, and shows stronger inhibition against Malassezia and less impact on Staphylococcus epidermidis in a Malassezia vs. symbiotic bacteria comparison model.

Certain intestinal or commensal bacteria (produced naturally or in vitro) induce Th17 cells, leading to production of IL-17, reducing lipid absorption, and thereby counteracting metabolic syndrome, obesity, and associated morbidities, such as type-2 diabetes, cardiovascular disease, and NASH / NAFLD. Administration of those bacteria, or of Th17 cells induced by those bacteria, to a subject helps counteract metabolic syndrome and associated morbidities. Antagonists of intestinal CD36 or molecules decreasing intestinal CD36, e.g., IL-17, may also be used to reduce lipid absorption. Depletion of Faecalibacterium rodentium or members of the Erysipelotrichaceae family in the subject may provide a similar result. ILC3 or IL-22 blockade (alone or combined with Th17 cell administration or induction if not already present in the subject), may further provide a similar result, protecting against metabolic disease.

The invention relates to ackermann mucophilic bacteriophage AkkZT003P and application thereof, and belongs to the technical field of microorganisms. The phage provided by the invention is Akkermansia muciniphila phage AkkZT003P, the phage is preserved in the Guangdong Microbial Culture Collection Center on January 6, 2025, and the preservation number of the phage is GDMCC (China General Microbiological Culture Collection Center) NO: 65724-B1. According to the invention, Ackermann mucophilum and Ackermann mucophilum bacteriophage are successively separated from excrement through in-vitro experiments, and animal experiments prove that the bacteriophage can crack Ackermann mucophilum to maintain the integrity of mucus. The effect of the bacteriophage on Ackerman muciniphile can lead to increase of downstream symbiotic bacteria streptococcus thermophilus, so that the intestinal barrier is further protected, the intestinal inflammation is improved, and the intestinal health is recovered.

The invention provides application of parabacteroides dieldrii in preparation of a medicine for treating Parkinson's disease. The parabacteroides dieldrii can play a neuroprotective role through multiple mechanisms of regulating intestinal flora, reducing neuroinflammation, improving a blood-brain barrier function and the like. Animal experiments show that the active parabacteroides dielsii can significantly improve PD mouse dyskinesia, protect dopaminergic neurons, repair intestinal and central barriers, and remodel a metabolite spectrum related to nerve regulation. The intestinal symbiotic bacterium parabacteroides dieldrii provided by the invention has a good effect of treating the Parkinson's disease, and has a wide application prospect in the field of micro-ecological treatment of the Parkinson's disease.

The invention discloses a flora-friendly selective antifungal peptide RVH and application thereof, and belongs to the technical field of novel antibacterial materials. The antifungal peptide RVH can resist pathogenic fungi on the premise of maintaining flora balance, drug resistance is not prone to being generated, the antifungal peptide RVH has high biological safety and has a certain inhibition effect on matrix metalloproteinase, and more importantly, the antifungal peptide RVH only selectively resists pathogenic fungi instead of bacteria, and especially does not resist symbiotic bacteria.