190 results about "Symbiotic bacteria" patented technology

A method for directly seedling dendrobe seedlings by using a plastic greenhouse comprises the following steps: constructing a greenhouse; making a seedling bed; preparing a base material and performing sterilization, which can be realized by mixing pine barks and sheep manure, filtering the pine barks and the sheep manure through a screen after completely fermenting the pine barks and the sheep manure, soaking the pine barks and the sheep manure by using carbendazim, spreading the pine barks and the sheep manure on the seedling bed, placing the pine barks and the sheep manure on the seedling bed for 5-10 days, and controlling the water content; inoculating the symbiotic bacteria, which can be realized by taking a two years old base material of the dendrobe and uniformly scattering the two years old substrate on the seedling bed on which a new substrate is laid; pre-treating seeds, which can be realized by completely moisturizing the seeds by using sterile water containing naphthyl acetic acid; sterilizing the seeds in the greenhouse, which can be realized by fumigating every cubic meter of seeds by using 4 g mushroom protector, fumigating the seeds for one time before sowing, and fumigating the seeds every half a month in the future; scattering, which can be realized by after the seeds are scattered and before the seedlings are transplanted, controlling temperature in the greenhouse, water content of the seedling bed and air humidity, treating the seeds by weak lights within the first month of the scattering, and gradually enhancing illumination after budding; managing water, fertilizers and diseases of the seedlings; and transplanting the seedlings. The method for directly seedling the dendrobe seedlings by using the plastic greenhouse, provided by the invention has the advantages that as the dendrobe are transplanted after directly becoming to the seedlings, the seedling breeding cost is effectively reduced, the seedling rate of the seeds is more than 90 percent, and the transplanting survival rate of the seedlings is 100 percent.

The invention discloses a Pteromalus puparum venom serine protease inhibitor Pp-PI polypeptide which has an amino acid sequence shown in SEQ ID NO: 2. At the same time, the invention also discloses a gene for encoding the Pteromalus puparum venom serine protease inhibitor Pp-PI polypeptide. The gene has a nucleotide sequence from the 126th nucleotide to the 290th nucleotide in SEQ ID NO: 1; or the gene has at least 70% homology with the nucleotide sequence from the 126th nucleotide to the 290th nucleotide in SEQ ID NO: 1; or the gene has a nucleotide sequence which can hybridize with the nucleotide sequence from the 126th nucleotide to the 290th nucleotide in SEQ ID NO: 1 at 40-55 DEG C. The Pteromalus puparum venom serine protease inhibitor Pp-PI polypeptide can be used to prepare a Pteromalus puparum venom serine protease inhibitor for genetically modifying crops or altering plant symbiotic bacteria.

The invention relates to a wall-breaking technique of ganoderma lucidum spore, in particular to a biologic method for carrying out wall breaking on the ganoderma lucidum spore. The method comprises the following steps of: separating and purifying ganoderma endophytic bacteria from ganoderma; sieving strains; preparing enzyme liquid; carrying out enzymolysis on the ganoderma lucidum spore; and preparing wall-breaking ganoderma lucidum spore powder. The invention utilizes the self symbiotic bacteria of the ganoderma to carry out fermentation on the spore and a fruiting body of the ganoderma, the effective ingredients of the ganoderma more easily escape from cells, a human body can successfully absorb and digest the effective ingredients, and the effective ingredients can be fully utilized. The difficulty that biologic residues caused by introducing foreign bacteria are hard to remove can be eliminated, and the safety of a product is ensured.

An isolated protein which is structurally and functionally similar to avidin but with improved properties, such as better affinity towards biotin conjugate, useful immunological properties or faster biotin dissociation rate, compared to avidin and streptavidin.

Among the fewer than 10 proteins primarily secreted by the species Lactobacillus plantarum, there is one of 30 kDa that contains an internal fragment without cleavage sites for the most important intestinal proteases and characterized by having a serine and threonine content of at least 50%. The genetic information encoded in this fragment, designated ST peptide, has been used for producing and purifying said peptide, thus making it possible to conduct various tests in vitro.To summarize, the ST peptide is considered to promote the process of immunologic ignorance of our gastrointestinal immune system toward the commensal bacteria of our gastrointestinal tract, thus favoring the mechanisms of oral tolerance. Therefore the ST peptide could be used in immunotherapy, especially in the context of certain autoimmune diseases and certain inflammatory diseases.

The invention provides a preparation method of steinernema carpocapsae. The steinernema carpocapsae belongs to steinernematidae of rhabditida of phasmida, and is one of the entomopathogenic nematodes which are most widely and potentially applied to biological control. Symbiotic bacteria exist in bodies of the entomopathogenic nematodes and enter an insect along with the nematodes to result in blood poisoning which causes the death of the insect; and the steinernema carpocapsae has a unique advantage and a good control effect on control of concealed pests such as borers and soil pests.

An oral treatment / care agent including the combination of one or more of an oral bacterial flora-controlling agent that will restore abnormal or sick oral bacteria flora to a normal or healthy oral bacterial state and a periodontal-pocket bacterial flora-controlling agent that will eliminate or convert the bacteria, fungi, viruses and other microbes in a periodontal pocket into a group of symbiotic bacteria. This agent is capable of preventing diseases of the oral tissue and is effective in stopping or slowing the development of a disease in progress.

The invention discloses a soilless planting method for organic tomatoes. The soilless planting method comprises the following steps: performing sterilization treatment on non-transgenic tomato seeds without chemical treatment, and performing culture by using an organic fertilizer obtained after fermentation and decomposition of straws as a substrate and performing irrigation with a leachate in the whole process; and adopting a culture mode of cyclic fertilization and timely liquid fertilizer recovery in combination with management of the tomatoes at different stages. The invention also provides an organic substrate and an organic liquid fertilizer for planting vegetables and preparation methods of the organic substrate and the organic liquid fertilizer, wherein straws and chicken manures are used as raw materials, and prior period fermentation, late period decomposition and continuous wet compost are performed by using characteristics of symbiotic bacteria; the leachate is continuously extracted and supplied, and the organic liquid fertilizer is obtained by collection; and the organic liquid fertilizer is comprehensive in nutrient, balanced in fertilizer effects, and beneficial for absorption by plants. The method disclosed by the invention achieves aims of high yield and high quality, and has relatively great economic values, so that the method is an efficient, practical and low-cost organic planting method.

The present invention relates, e.g., to a commensal bacterium which can colonize the genitourinary and / or gastrointestinal mucosa, and which, under suitable conditions, secretes a heterologous antimicrobial polypeptide, wherein the secreted antimicrobial polypeptide is effective to inhibit infectivity by, or a pathogenic activity of, a pathogen. In a most preferred embodiment, the antimicrobial polypeptide inhibits HIV infection (e.g., fusion) and / or pathogenesis. Also described are preventive or therapeutic compositions comprising the commensal bacteria, and methods to inhibit infectivity and / or pathogenesis, using the bacteria.

The invention provides a tremella liquid cultivar culture method and a special culture medium for a cultivar. The tremella liquid cultivar culture method includes the following steps that firstly, bevel culture media are used for culturing Hypoxylon sp and tremella bacteria respectively; secondly, strains of the Hypoxylon sp and strains of the tremella bacteria are moved into the liquid culture media for culture respectively; thirdly, the liquid strains of the Hypoxylon sp are mixed into the liquid strains of the tremella bacteria according to the volume percent of 1-5% and inoculated into a culture bottle for culture, and the cultivar is obtained through preparation. According to the tremella liquid cultivar culture method, the problem that a traditional cultivar is difficult to prepare is solved, meanwhile, the liquid strains are faster in mycelia running, matched growth of the liquid strains and symbiotic bacteria can be effectively achieved, and the yield of tremella produced by the liquid cultivar prepared with the method is improved by more than 20% compared with the yield of tremella produced by the traditional cultivar.

Disclosed is an immunopotentiating composition comprising an effective immunostimulating substance which can activate natural immunity and a subsequent immunity mediated by lymphocytes without causing any damage to cells. Specifically disclosed is an immunopotentiating composition which is characterized by comprising, as an active ingredient, an immunostimulating substance produced by decomposing at least one bacterium selected from an MRE symbiotic bacteria group consisting of Bacillus sp. (FERM BP-11209), Lysinibacillus fusiformis (FERM BP-11206), Bacillus sonorensis, Lysinibacillus sp. (FERM BP-11207) and Comamonas sp. (FERM BP-11208).

The invention discloses a sterilization composition for regulating skin microorganism floras and an application of the sterilization composition. The sterilization composition consists of a lactobacilli fermentation lysis product, quaternary ammonium salt-73, and a mangosteen peel extract. For the composition, the lactobacilli fermentation lysis product and the mangosteen peel extract can reduce killing rate of beneficial bacteria or symbiotic bacteria when other small pox removing components (such as the quaternary ammonium salt-73) are used for killing Propionibacterium acnes, and unbalanceof different degrees of floras caused by individual difference is balanced. In addition, the collocation use of the lactobacilli fermentation lysis product and the mangosteen peel extract can also effectively reduce stimulation or damage of conventional preservatives to faces of acne patients, and can reduce generation of allergic reaction situations that faces are itching, painful and swollen caused by preservatives.

The invention discloses an application of fermenting technology and fermenting material of entomiasis initial line insect symbiosis fungus. It uses Xenorhabdus nematophilus YL001 as raw material and builds a fermenting technology that could improve the antibacterial activity and high biological yield. The fermenting product of Xenorhabdus nematophilus YL001 could be made into entomiasis initial line insect symbiosis fungus antiseptic for controlling cayenne pestilence mildew, and the effect is good.

The invention provides a microbial mixed flora used for fermenting to produce 1,3-propanediol by using glycerin as a substrate and a fermentation method. The microbial flora mainly contains clostridium butyricum (95 percent or above), and in addition, contains mixed symbiotic bacteria (5 percent or below) of a clostridium bacterial strain and a lactobacillus bacterial strain; the proportion of the effective viable counts of the clostridium bacterial strain and the lactobacillus bacterial strain in the mixed symbiotic bacteria is (1.50 to 4.99) : (0.01 to 0.05). In comparison with the fermentation of a single bacterium, the microbial mixed flora is good in fermentation stability, is high in biological safety, and has the advantages that the tolerance of the crude glycerin substrate is high, the production intensity is high, the sterilization is not needed in a production process, and the like; a novel fermentation mode is provided for the industrial production of the 1,3-propanediol. In the batch type fed-batch fermentation in which the rough glycerin is used as the substrate, the concentration of the 1,3-propanediol can reach 79.78g / L; the molar conversion ratio is 0.65mol / mol; the production intensity is 3.32g / (L*h).

The invention discloses a specific primer pair for assisting identification of Klebsiella pneumoniae. The primer pair provided in the invention consists of DNA shown in sequence 1 of the sequence table and DNA shown in sequence 2 of the sequence table. The specific primer pair designed in the invention can be used for assisting identification about whether Bactrocera dorsalis (Hendel) carries Klebsiella pneumoniae only by means of PCR (polymerase chain reaction) and electrophoresis, and the identification is characterized by high sensitivity, simple process and no need for cloning. In order to increase accuracy, a PCR amplified product can be subjected to sequencing. The specific primer pair provided in the invention improves the efficiency of Bactrocera dorsalis (Hendel) symbiotic bacteria (Klebsiella pneumoniae), and creates conditions for further study of the coevolution relation between Bactrocera dorsalis (Hendel) and Klebsiella pneumoniae.

The present invention is termite resisting method of utilizing metabolic product of tree endophyte to inhibit symbiotic bacteria of termite. The present invention includes separating symbiotic bacteria with lignin and / or cellulose decomposing capability from termite body as the indication bacteria; separating tree endophyte from termite tree and fermenting to obtain metabolic product of tree endophyte; performing bacteriostasis test with the metabolic product of tree endophyte on symbiotic bacteria via Oxford process; and soaking filter paper with the tree endophyte with bacteriostasis on the symbiotic bacteria and feeding termite with the soaked test paper. Statistics shows that the termites fed with the filter paper has obviously higher death rate and obviously lower activity, and this probes that the fermented liquid contains termite resisting compound.

The present disclosure describes compositions and methods for increasing the abundance of commensal bacteria belonging to the order Clostridiales, including Blautia, Ruminococcus, Clostridium, Eubacterium, Holdemania and Dorea species, that are as-sociated with reduced lethal GVHD and improved overall survival following bone mar-row or hematopoietic stem cell transplant. The present disclosure, therefore, provides methods for reducing the likelihood, incidence or severity of GVHD by (1) avoiding the loss of endogenous beneficial species through antibiotic selection; (2) by administering a therapeutically effective amount of a composition comprising one or more Clostridiales associated with reduced GVHD to individuals who may lack or have lost those strains from their intestinal microbiota. Additionally, support for endogenous or reestablished Clostridiales related to reduced GVHD as a treatment option for reducing GVHD can also be provided in the form of nutritional supplementation, for example, sugars fermented by some species of Clostridiales with GVHD reducing activity.

The invention discloses a method and special strain for cultivating epiphytic orchid. The strain provided by the invention is pseudocercospora sp. S3CGMCC No. 4148. The strain has an obvious effect of promoting seedling growth, can be used as high-quality symbiotic bacteria for growth of dendrobium nobile and can be applied to gardening and planting production of the plant. The strain has a broad application prospect in conservation and large-scale propagation of other orchid plants, particularly epiphytic orchid plants.

The invention relates to the field of microalgae symbiotic bacteria isolation, specifically speaking, relates to a microalgae symbiotic bacteria isolation medium and separation method, and a high-throughput screening method for key bacteria affecting the growth of microalgae. Improvement can be performed based on a common marine bacterial isolation medium - Zobell 2216E marine broth bacterial culture medium, so that the culture medium for increasing the culturable bacterial species of microalgae phycosphere symbiotic bacteria can be provided, and abundant strain resources can be provided for the screening of key bacteria affecting the growth of microalgae. Through the high-throughput rapid screening method for significantly promoting or inhibiting algae growth bacteria, bacteria having keyinfluences on the growth of the microalgae can be discovered in a short time.

The invention relates to a multiple PCR detection method for detecting Wolbachia and Cardnium symbiotic bacterias in tetranychus truncatus ehara body at the same time, belonging to the field of agricultural biotechnologies; the multiple PCR is an improved PCR technology on the basis of a common PCR, a plurality of pairs of auele specific primers are added in a PCR reaction system, aiming at different areas of a plurality of DNA templates or the same template, a plurality of purpose fragments are amplified. According to Wolbachia wsp gene sequence and Cardnium 16S rDNA gene sequence, two pairs of new auele specific primers are designed, and two purpose fragments - 211bp and 279bp which has different size, are amplified. The multiple PCR detection method has high specificity, sensibility, feasibility and effectiveness, namely, the detecting cost is saved and the template is saved. The multiple PCR detection technology for detecting the Wolbachia and Cardnium symbiotic bacterias at the same time can be widely applied to the detection of the two symbiotic bacterias in tetranychus truncatus ehara.

The invention provided a detection method of a flora of symbiotic bacteria which is Wolbachia bacteria in mymanidae bodies. Specifically, the method comprises steps that: total DNA in a mymanidae sample is extracted; genetic segments of 16SrDNAV3 hypervariable regions of Wolbachia bacteria, total bacteria, and total bacteria except Wolbachia bacteria in the mymanidae sample are amplified through nested PCR; DGGE (denaturing gradient gel electrophoresis) analysis is carried out; and the Wolbachia bacteria in the mymanidae sample can be detected through DGGE spectrum analysis, and through the recovering, cloning and sequencing of the DGGE strap. The invention provides a relatively rigorous PCR-based technological method for detecting Wolbachia bacteria in mymanidae bodies. Also, with the method, varieties of Wolbachia bacteria in mymanidae bodies can be rapidly and accurately determined. Therefore, the method has an important promoting significance and a practical value on current detections and related researches of symbiotic Wolbachia bacteria in insect bodies.

To provide a method for raising plants that have great vitality and that are strongly resistant to pests and can successfully compete against weeds. The invention provides a method for raising plants with enhanced immunity and biological activity to external and internal stresses. The present invention is characterized by the following: the method includes a step of applying to the target plants a plant immunopotentiating composition containing an immunostimulatory substance produced by the cytolysis that accompanies spore formation in aerobic endospore-forming bacteria; the immunostimulatory substance is obtained by culturing the aerobic endospore-forming bacteria, causing the bacteria to form endospores by placing the resulting culture liquid in starvation conditions, and then removing impurities including the bacterial cells that are formed into endospores from the culture liquid; and the aerobic endospore-forming bacteria belong to the MRE symbiotic bacteria group.

The invention discloses a solid culture method for entomopathogenic nematodes. The method is characterized by comprising the specific steps: (a) preparing an inoculum culture medium, activating symbiotic bacteria of the entomopathogenic nematodes, and inoculating the symbiotic bacteria to the inoculum culture medium; (b) inoculating a bacterial solution of the symbiotic bacteria to a solid culture medium; (c) carrying out culture for 3 to 5 days in the solid culture medium, and then, inoculating an entomopathogenic nematode suspension to the solid culture medium; (d) carrying out dark static culture on the nematodes for three weeks, thereby harvesting the nematodes. According to the method, the problem that bioculture methods cannot achieve the large-scale production of the entomopathogenic nematodes is solved, the production cost of the solid culture method is obviously lower than that of a liquid culture method, and the pathogenicity of the nematodes produced by the solid culture method is apparently higher than that of the nematodes produced by the liquid culture method. The market demand is increased, and the solid culture method has better prospects of development and has an all-important significance in the large-scale production of the entomopathogenic nematodes and the biological control on soil insects.

The invention provides a detection method of symbiotic bacteria in sponge cells based on quantum dot fluorescence in-situ hybridization, which relates to the technical field of bacterial detection. The detection method comprises the following steps of: obtaining a sponge single cell; according to a classical fluorescence in-situ hybridization method, adopting an oligonucleotide probe marked by biotin to hybridize microbial genome DNA (deoxyribonucleic acid) in the sponge single cell; finally, adopting a quantum dot marked by streptavidin to carry out incubation treatment; and obtaining blue sponge cells in a fluorescence microscope with exciting light as an ultraviolet band and red bacteria. The invention combines a mechanical method and a chemical method, can quickly obtain sponge cells, avoids the autofluorescence interference of the sponge cells and successfully detects the bacteria in the sponge cells.

The invention provides a nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin, essence lotion, a preparation method of the nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin, and an application of the essence lotion. The nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin comprises the symbiotic bacterium combined fermented products, decarboxy carnosine HCl, palmitoyl pentapeptide-4, tocopheiyl acetate, a pale butterflybush flower extract, resveratrol, a pomegranate fruit extract and a narcissus bulb extract, wherein symbiotic bacteria of the symbiotic bacterium combined fermented productscomprise combination of schizophyllum commune, thermus thermophilus, microzymes and lactobacilli, and fermentation raw materials of the symbiotic bacterium combined fermented products comprise combination of rice, coix lacryma-jobi ma-yuen seeds, ginseng, kelp and raspberries. Through synergistic compounding of effective active components of the symbiotic bacterium combined fermented products andthe like, the nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin can condition and repair skin ageing resistant routes of oxidation resistance, saccharification resistance, blue light resistance, collagen synthesis promotion and the like, so that cell vitality is protected, and the skin has the capacity for resisting exogenous environmental factors.

The invention discloses an entomopathogenic nematode symbiotic bacteria strain which is characterized in that the class of the microorganism is named high-virulent strain R187-2 of serratia nematodiphila and collected in the China Center for Type Culture Collection on May 3rd, 2016, wherein the strain collection number is CCTCC M 2016240. The invention also discloses a biological insecticide as well as a preparation method and application thereof. The novel entomopathogenic nematode symbiotic bacteria insecticidal bacterial agent provided by the invention has the advantage of convenient production and use and is suitable for producing green pollution-free agricultural products in the modern ecological agriculture economy. The entomopathogenic nematode symbiotic bacteria strain is of great significance to protecting the ecological environment and improving the added value of agricultural products. Experiments prove that the liquid bacterial agent and solid bacterial agent of the R187-2 strain realize a good insecticidal effect, and the death rate of each group is 85% or more. With a wide insecticidal spectrum, the entomopathogenic nematode symbiotic bacteria strain R187-2 has a huge market development potential.

The invention discloses a soilless half-hydroponic planting method of organic Chinese chives, and belongs to the field of facility agriculture cultivation. Disinfection treatment is conducted on non-transgenic Chinese chive seeds which are not subjected to chemical treatment, and in the whole process, organic fertilizers such as fermented and decomposed straw and chicken mature are utilized for matrix cultivation and leaching agent irrigation; seedling is conducted in the earlier stage of 3 months to 4 months; transplanting is conducted in the middle stage of 6 months to 18 months for half-hydroponic cultivation; in the later stage, hydroponic cultivation is conducted by combining an order, and cyclic fertilizer supply and timely liquid fertilizer recycling are adopted; a cultivation mode that the Chinese chives are managed in different periods and stages is combined. The invention further provides an organic matrix for planting vegetables and organic liquid fertilizer and a making method thereof. Earlier fermentation, post decomposition and continuous retting are conducted on straw, chicken manure and the like which serve as the raw materials by means of the characteristic of symbiotic bacteria; continuous extracting and leaching agent supplementing are conducted, and the organic liquid fertilizer is obtained after collecting is conducted; nutrients are comprehensive, the fertilizer efficiency is balanced, and plant absorption is promoted; the soilless half-hydroponic planting method of the organic Chinese chives is an efficient, practical and low-cost planting method.

The invention discloses a breeding method for vanda. The breeding method comprises the following steps of: selecting a full terminal bud which is germinated in the current year as a material; removing redundant leaves for induction culture; germinating strong buds after 15-20 days; carrying out enrichment culture on the buds for 20-30 days; and performing root induction on the buds for 20-25 days to obtain seedlings for transplanting, wherein the survival rate is higher than 95%. The expanding propagation coefficient is high, and nursery-grown plants grow well after surviving. The vanda is single-axis stem cattleya, so that lateral buds are almost not generated. Moreover, stem tips contain many polyphenols with strong activity, and the cut stem tips are easy to brown during the culture, so that the culture is difficult to succeed; and natural sexual propagation is difficult to succeed as seed germination depends on symbiotic bacteria nourishing. The technical scheme provided by the invention solves the problems of low survival rate, slow propagation and the like in the prior art, and has remarkable economic and social benefits.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a growth medium capable of promoting seed germination and seedling growth of bletilla striata, a formula of the growth medium and a preparation method. The preparation formula of the growth medium capable of promoting seed germination of bletilla striata comprises the following components: MS or 1 / 2 MS, 0.1-2.0 mg / L of NAA (naphthalene acetic acid), 0.8-4.0 g / L of activated carbon, 40-60 g / L of mashed potatoes, 40-60 g / L of mashed bananas, 1-7 g / L of perilla stem, 2-5 g / L of clematis chinensis, 3-10 g / L of uniflower swisscentaury root, 2-9 g / L of notopterygium incisum, 3-6 g / L of viola mandshurica, 2-5 g / L of lophatherum gracile, 1-4 g / L of vietnamese sophora root, 2-5 g / L of radix asparagi and 4-7 g / L of reed rhizome. The growth medium has the advantages as follows: MS or 1 / 2 MS is taken as the basic growth medium; NAA promotes seed germination; the mashed bananas and the activated carbon facilitate seedling strengthening and rooting; seed germination and seedling growth can be directly promoted by joint application of perilla stem, clematis chinensis, uniflower swisscentaury root and notopterygium incisum; growth of bletilla striata symbiotic bacteria can be promoted by viola mandshurica, lophatherum gracile, the vietnamese sophora root, radix asparagi and reed rhizome, and seed germination and seedling growth are promoted indirectly.