The invention relates to the field of milk powder manufacturing, and in particular to novel milk powder and a manufacturing method thereof. The milk powder comprises the following components in partsby weight: OPO, whey protein powder (containing milk fat globule membranes and cephalin groups), raw milk, yak milk or whole yak milk powder, oligosaccharide, choline, DHA and lutein, and also comprises SN-2, wherein the content of the SN-2 is 60% or above of the weight of total palmitic acid in the milk powder. The OPO structural fat used in the novel milk powder is a new generation OPO, and thenovel milk powder has SN-2 close to the SN-2 level of breast milk, is beneficial to digestion and absorption of babies, and can improve energy intake, reduce constipation, enhance bone density, increase intestinal tract probiotics, reduce crying and improve night sleep. The novel milk powder can also improve cognitive ability, learning ability and memory of infants, can promote growth of probiotics, enhance the immune function of intestinal tracts, improve the stool character of the infants, reduce occurrence of constipation, and promote absorption and utilization of various nutrient substances.