103 results about "Intestinal immunity" patented technology

The invention provides an Akkermann Amuc1100 protein mutant as well as a preparation method and application thereof, wild Akkermann Amuc1100 protein is subjected to mutation modification through a molecular biological technical means, and the bioactivity of the Amuc1100 protein mutant subjected to molecular modification is obviously improved compared with that of the wild protein; compared with a wild type Amuc1100 protein, the wild type Amuc1100 protein has a better effect when being applied to the aspects of regulating intestinal immunity, relieving acute pancreatitis, promoting browning of white adipose cells, inhibiting obesity, relieving ulcerative colitis and the like, and a very good application prospect is provided for developing bioactive medicines with higher efficiency.

The invention provides a patient consultation intelligent reply method and system based on an RAG knowledge base, and is applied to the technical field of data processing. The method comprises the following steps: preprocessing multi-modal data of a patient to generate text semantic features, voice features, intestinal barrier function features, intestinal immune function features, intestinal nerve function features and image features; performing feature extraction processing on the historical dialogue data of the patient to generate past consultation features and medication feedback features; processing the previous consultation features and the medication feedback features to generate patient portrait information; preprocessing the medical knowledge base data, extracting preset semantic features and storing the preset semantic features to a mixed index structure; the intestinal barrier function features, the intestinal immune function features and the intestinal nerve function features are processed, and intestinal influence factors are generated; and processing the text semantic features, the image features, the patient portrait information and the intestinal tract influence factors based on the dynamically updated medical knowledge base data and an RAG technology to generate target reply information.

The invention provides a feed additive capable of improving immunity of pet cats as well as a preparation method and application of the feed additive, and belongs to the technical field of animal feed additives. The feed additive comprises probiotics and a traditional Chinese medicine extract, the probiotics comprise bifidobacterium animalis, combined lactobacillus salivarius and saccharomyces cerevisiae boulardii, and the traditional Chinese medicine extract comprises astragalus polysaccharide, curcumin and resveratrol. The probiotics directly enhance intestinal immunity through colonization resistance and metabolites, the traditional Chinese medicine components form internal and external dual defense through system immune activation, immunity-flora dual regulation is achieved, the probiotics and the traditional Chinese medicine extract form a symbiotic system of increasing the drug effect through bacteria and protecting the bacteria through drugs, and 1 + 1gt is achieved; and 2, the immunity improving effect is achieved.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

The invention provides a feed for relieving weaning stress of lambs and a preparation method of the feed, and belongs to the field of animal feeds. The feed disclosed by the invention is prepared from the following components in parts by weight: 35-40 parts of puffed corn, 15-20 parts of enzymolysis soybean meal, 5-8 parts of fish meal, 5-8 parts of alfalfa meal, 0.3-3 parts of an organic trace element compound, 0.1-2 parts of a vitamin compound, 3-5 parts of whey powder, 0.5-1 part of butyrin, 0.3-0.5 part of glutamine and 0.2-0.3 part of a radix astragali-spina date seed compound extract. 0.2 to 0.3 part of xylooligosaccharide-xylanase compound, 0.05 to 0.1 part of pancreatin active peptide and 0.1 to 2 parts of complex microbial inoculant. The weaning stress of the lambs is reduced through a targeted intestinal tract-immunity-nerve regulation ternary synergistic formula, the digestive system development of the weaned lambs can be obviously improved, the activity of digestive enzymes can be improved, and therefore the weight gain of the lambs is obviously improved, and the lambs stably pass through the weaning period.

The invention discloses a plant lactobacillus PC037 capable of improving intestinal immunity and a fermented product. The plant lactobacillus PC037 is preserved in the China General Microbiological Culture Collection Center on December 13, 2021, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 24096. The plant lactobacillus PC037 can improve the repair speed of the intestinal mucosa and promote the enhancement of the immunity of the intestinal tract. The plant lactobacillus fermented product capable of improving the immunity of the intestinal tract, provided by the invention, can be used for adjusting the exhaust frequency, promoting the enhancement of the immunity of the intestinal tract and reducing the occurrence frequency of diarrhea caused by pathogenic bacteria infection of travelers in a travel process.

The invention discloses an enzymolysis preparation method of high-immunocompetence bovine coloctrum peptide, active peptide and application thereof, and belongs to the field of biotechnology and functional foods.The enzymolysis preparation method is characterized by comprising the steps that bovine coloctrum is degreased, trehalose is added into obtained supernate, first stirring is conducted, and first mixed liquid is obtained; wherein the supernate comprises whey protein and Ig; adding medium protease into the first mixed solution for hydrolysis to obtain enzymatic hydrolysate; wherein the enzymatic hydrolysate comprises a mixture of bovine colostrum hydrolyzed peptides, the Ig activity retention rate is larger than or equal to 90% through the targeted protection and directional enzymolysis technology, the beta-lactoglobulin peptide with the synergistic immune enhancement effect is obtained, the Ig and small molecule peptides in the product have the synergistic effect, the body immune regulation capacity and the intestinal immune function are remarkably improved, the immunity of the human body is improved, and the immunity of the human body is improved. The method can be widely applied to the fields of infant formulas, postoperative nutrition, functional drinks and the like.

The invention relates to an establishment method and application of in-vitro intestinal inflammation models of mice of different ages, and relates to the technical field of biology. According to the invention, young, adult, middle-aged and old wild type (WT) and TLR4 gene deletion type (TLR4 <- / ->) mice are selected, colon tissues are cultured in vitro, inflammatory response is induced by using interleukin-1beta (IL-1beta) stimulation, an age-related intestinal inflammation model is constructed, the inflammation phenotype of the mouse model and the activity of a TLR4 / MyD88 / NF-kappa B signaling pathway are determined by comparing WT and TLR4 <- / ->, and the age-related intestinal inflammation model is constructed. The core function of the TLR4 in regulating age-dependent intestinal barrier dysfunction is defined, a regulatory network of the TLR4 in intestinal immune aging and flora-host interaction is disclosed, and an age personalized medical strategy is promoted. According to the invention, an efficient and customizable multi-age in-vitro intestinal inflammation model is constructed, and an innovative platform is provided for mechanism analysis and intervention drug screening of TLR4 targeted therapy and age-related intestinal diseases.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention belongs to the field of food nutrition and functional factors, and particularly relates to a method for preparing ovotransferrin hydrolysate with high immunocompetence based on an adult gastrointestinal simulated digestion system. According to the method, ovotransferrin is treated through the stomach and intestine of a simulated adult, OVT hydrolysate is prepared, and the OVT hydrolysate contains HTEGSTT, TTSY and / or RTAGWVIPMG. The invention finds that the immunomodulatory activity of OVT is enhanced after gastrointestinal digestion and hydrolysis, the OVT is more easily hydrolyzed in simulated adult digestive juice than in infant digestive juice, and a contrast experiment shows that the nutrition intervention of OVT adult and infant simulated digestion products can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid; the swelling injury caused by inflammation to small intestine mucous membrane tissues is effectively relieved, so that the intestinal barrier immune function is obviously improved, and the OIA shows higher intestinal immune activity than the OIB.

The invention provides a composition for promoting differentiation of intestinal immune cells as well as a preparation method and application of the composition. According to the composition, 1, 3-dioleic acid-2-palmitic acid triglyceride and breast milk oligosaccharide are used in a combined manner, so that monocytes can be effectively promoted to differentiate into intestinal macrophages, and inflammation is reduced to a certain extent. After the monocytes are differentiated into the intestinal macrophages, the intestinal immune homeostasis and intestinal microbiota inflammatory response can be regulated, so that the mucosal immune homeostasis and intestinal epithelial cell barrier function are maintained. Therefore, the composition provided by the invention can improve the intestinal immune barrier by promoting the monocytes to differentiate into the intestinal macrophages, and provides a new thought for improving the early intestinal barrier of infants through nutritional supplement.

The invention provides a preparation method and application of polygonatum cyrtonema acidic polysaccharide, and belongs to the technical field of polygonatum cyrtonema polysaccharide extraction. According to the invention, a separation and purification method combining anion exchange chromatography with gel chromatography is adopted, and finally the polygonatum cyrtonema acidic homogeneous polysaccharide (PCPA) with relatively high purity is obtained. An in-vivo pharmacodynamic experiment shows that the prepared PCPA can remarkably relieve intestinal mucosa injury and has relatively high intestinal immune regulation activity. The preparation method provided by the invention is stable in process, and the prepared polygonatum cyrtonema acidic polysaccharide is high in purity, is a homogeneous polysaccharide and has the effect of regulating intestinal immunity.

The present application relates to a kind of composition for adjusting intestinal flora and its preparation method and application, and the raw materials of the composition include: fried atractylodes, dried tangerine or orange peel, fried liquorice, magnolia officinalis (ginger preparation), polyporus umbellatus, poria cocos (skin removal), fried atractylodes, alisma orientalis, cassia bark, dried ginger, dark plum.The present application produces the effect of anti-inflammatory, antioxidant, regulating intestinal flora, improving intestinal immunity and intestinal barrier protection effect etc. through the synergistic effect between raw materials.

The invention relates to livestock feeding probiotics, in particular to bacillus subtilis and application, the bacillus subtilis is bacillus subtilis BW and is preserved in the China General Microbiological Culture Collection Center on April 10, 2025, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) 0.34165. The bacillus subtilis BW disclosed by the invention can be colonized in animal bodies, so that the diarrhea rate of piglets is remarkably reduced, the immunity of intestinal tracts is improved, and the health of the intestinal tracts is improved; after the bacillus subtilis BW is used for feeding ruminants, the feed conversion efficiency can be remarkably improved, the growth performance can be improved, and the bacillus subtilis BW has a good application prospect in livestock and poultry breeding.

The invention discloses a clostridium praeparatum vesicle targeting inflammatory bowel disease tissues as well as preparation and application of the clostridium praeparatum vesicle. The vesicle is secreted by clostridium praeparatum ATCC27766, and the average particle size of the vesicle is 100-250 nm. Experimental data show that the vesicles have intestinal disease site targeting, can effectively remodel the intestinal immune microenvironment of ulcerative colitis patients, and inhibit the inflammation process through chemotactic factor secretion of macrophages. The clostridium praeparatum vesicle and the preparation can effectively overcome the defect that clostridium praeparatum and butyrate secreted by the clostridium praeparatum are prone to failure, the treatment means of ulcerative colitis are enriched, and the application prospect of anaerobic probiotics is widened.

The invention relates to the technical field of chemical toxicology, in particular to an evaluation method for comprehensively analyzing the immune induction effect of a plasticizer by using caenorhabditis elegans, according to the evaluation method, a mother worm breeding mode is changed to replace a lysis solution to synchronize nematodes, experimental interference is reduced, the result accuracy is improved, monitoring indexes are newly increased, intestinal injuries and in-vivo bacterial indexes are increased, and the evaluation method is suitable for comprehensive analysis of the immune induction effect of the plasticizer. The evaluation is more accurate and reliable by combining oxidative stress indexes and performing two-dimensional comprehensive evaluation from an intestinal immune defense line and oxidative stress, and by drawing a multi-index monitoring shape graph, adopting a comprehensive biomarker response index analysis method and associating immune-related representative indexes, the evaluation accuracy is improved. According to the method, the change of each index and the immunity intensity on the caenorhabditis elegans under different exposure conditions are intuitively presented, the immune defense effect of the caenorhabditis elegans under the stress of the plasticizer can be quickly and effectively evaluated without transcriptome and metabonomics, and efficient evaluation is realized.

The invention discloses a method for preparing a high-fiber gel minced fillet product based on low-molecular-weight tremella polysaccharide. The method comprises the following steps: modifying the low-molecular-weight tremella polysaccharide; dissolving the modified low-molecular-weight tremella polysaccharide in water to obtain tremella homogenate; and preparing the minced fillet: mixing fish meat with the modified low-molecular-weight tremella polysaccharide, adding table salt and auxiliary materials, and heating and curing to obtain the high-fiber gel minced fillet product. According to the tremella polysaccharide surimi composite product prepared by the preparation method disclosed by the invention, the modified tremella polysaccharide slurry is added in the surimi processing process, so that the gel performance of surimi can be well improved, the surimi has high fiber property, the smooth and elastic mouth feel of the surimi product can be enhanced, and the taste bud experience is richer. Meanwhile, as the tremella polysaccharide has rich biological activity, such as antioxidation, intestinal immunity regulation, blood sugar and blood fat reduction and the like, the tremella polysaccharide can be added into the surimi product as one of the raw materials, and additional functional characteristics can be endowed to the surimi product.

The application belongs to the technical field of biological nanomaterials, and particularly relates to an immune activation nanosheet as well as a preparation method and application thereof. The immune activation nanosheet comprises a two-dimensional montmorillonite nanosheet with a sheet layer structure, and manganese ions and tumor antigens loaded in the interlayer of the two-dimensional montmorillonite nanosheet. The mass ratio of the manganese ions to the montmorillonite is 1:5-1:20, and the mass ratio of the tumor antigens to the montmorillonite is 1:1-1:10. The immune activation nanosheet has the characteristics of good oral stability, high mucosal targeting, strong immune efficacy, etc. The stability of the immune activation nanosheet in the gastrointestinal tract is good, the immune activation nanosheet can be effectively enriched in the small intestine, a vaccine inoculation pool is formed, the intestinal immune tolerance can be overcome, the immune effect is enhanced, and the immune activation nanosheet has a clinical application prospect.

The invention discloses a bifidobacterium longum subsp. Infantis YLGB-1496 microbial agent, a preparation method and intestinal health application of the bifidobacterium longum subsp. Infantis YLGB-1496 microbial agent, and relates to the technical field of probiotics. The live bacterium agent of the bifidobacterium longum subsp. Infantis, provided by the invention, has the effects of resisting inflammation, effectively improving intestinal immunity, regulating intestinal flora and relieving intestinal flatulence, can be used for preparing fermented foods, medicines or feeds, and has a very wide application prospect.

The invention discloses a probiotic composition, fermented yoghurt and application of the probiotic composition and the fermented yoghurt in improving related indexes of athletes. The probiotic composition comprises bacillus subtilis SP-8-5, lactobacillus plantarum YM-4-3, lactobacillus plantarum AY01 and pediococcus acidilactici LH01. The composition of intestinal microbial populations can be adjusted through a specific compounding method, the abundance of harmful bacteria is reduced, the abundance of beneficial bacteria is improved, the immunity of intestinal tracts is enhanced, and the integrity of intestinal barriers of athletes is maintained. Meanwhile, the composition has a positive improvement effect on related exercise indexes such as plasma amino acid, excrement metabolites and exercise performance, and can be used as an effective exercise supplement to be applied to athletes.

The invention relates to a nutritional composition for improving an intestinal immune function and application thereof, and belongs to the technical field of microorganisms. The nutritional composition of the present invention comprises a bifidobacterium bifidum (Bifidobacterium bifidum), and a breast milk oligosaccharide. The breast milk oligosaccharide in the nutritional composition can provide a growth substrate for the bifidobacteria and promote colonization of the bifidobacteria in intestinal tracts. The bifidobacterium bifidum produces beneficial metabolites by metabolizing oligosaccharide in breast milk, and plays an immune regulation role. According to the nutritional composition, the intestinal immune function is remarkably improved, the intestinal flora structure is optimized, and the risk of immune-related diseases is reduced.

The invention belongs to the technical field of veterinary feed, and particularly relates to a feed additive for improving pork quality and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 30-40 parts of conjugated linoleic acid, 12-15 parts of gelatin, 10-15 parts of whey protein, 12-15 parts of yeast beta-glucan, 8-12 parts of butyrin, 6-7 parts of an antioxidant, 8-10 parts of chitosan and 40-60 parts of maltodextrin. According to the compound feed additive disclosed by the invention, through the synergistic effect of the conjugated linoleic acid microcapsules, the beta-glucan-butyrin compound and the tea polyphenol-chitosan compound, CLA slow release regulation and control of fat metabolism are realized, and the lean meat percentage and intramuscular fat are increased; beta-glucan can enhance intestinal immunity, and butyrin can promote nutrient absorption; the tea polyphenol-chitosan compound resists oxidation and improves the meat color and the water-retaining property, conventional methods such as spray drying and low-temperature mixing are adopted in the process, and the cost is effectively reduced.

The application discloses application of Gastrodia elata extract in preparation of a feed additive for resisting Aeromonas hydrophila infection, and belongs to the technical field of water animal disease prevention and treatment. The application first finds that the Gastrodia elata extract has a significant effect of inhibiting Aeromonas hydrophila infection. After the Gastrodia elata extract is added into water animal feed as a feed additive and is fed to the water animal, the Gastrodia elata extract is absorbed into blood circulation system through a digestive tract, up-regulates intestinal immunoglobulin gene expression, improves serum immunoglobulin level, enhances intestinal barrier function, improves animal activity, effectively resists Aeromonas hydrophila infection, reduces disease occurrence, and improves yield and quality of animal breeding. Therefore, the Gastrodia elata extract has a good application prospect.

The invention discloses a tea product for regulating and controlling bitter taste signals and immunity of intestinal tracts and application of the tea product, the tea product comprises green tea, black tea, oolong tea and Pu'er tea, and specifically, extracts are compounded according to a specific proportion. According to the invention, intestinal immunity is regulated and controlled by activating bitter taste signals in intestinal tracts, and no harm is caused to patients suffering from intestinal immune diseases and food anaphylaxis diseases; the traditional Chinese medicine composition is simple, convenient and rapid to use, and can obviously relieve intestinal immune diseases and food allergy.

The invention provides an application of a transcription factor PU.1 inhibitor or a pharmaceutically acceptable salt thereof in preparation of a medicine for treating ulcerative colitis, and belongs to the technical field of biological medicines. According to the invention, intervention is carried out from the upstream of an inflammation network through a small-molecule inhibitor specifically targeting a core transcription factor PU.1, and the multi-dimensional and synergistic treatment effect on ulcerative colitis is realized by regulating and controlling the functions of intestinal immune cells and maintaining the integrity of an intestinal barrier and the steady state of intestinal flora.

This invention discloses a naturally formed nutritional licking brick specifically for musk deer and its preparation method. Using calcium silicate as the skeleton material, it is compounded with yeast culture powder, bentonite, compound probiotics, sweeteners, and flavoring agents. The resulting licking brick continuously releases soluble nutrients and active bacteria during the musk deer's licking process. The specific combination of probiotics and yeast culture powder components effectively improves the intestinal immunity of captive musk deer, significantly reduces intestinal inflammation, and simultaneously lowers cortisol levels in feces, alleviating stress responses. Using yeast culture powder, calcium silicate, and bentonite as a composite coagulant, the licking brick prepared by mold casting exhibits optimal moisture resistance during storage. Furthermore, the licking brick has a smooth appearance, a compact and sturdy structure, excellent molding performance, and retains its structural integrity even after immersion in water, making it suitable for preparing products related to improving the intestinal health and immunity of musk deer.

The invention discloses a chitosan non-binding bilirubin microsphere as well as a preparation method and application thereof. The chitosan non-binding bilirubin microspheres are prepared by taking chitosan as a delivery carrier and taking chitosan and bilirubin as main raw materials through an emulsion reaction and a cross-linking reaction in sequence. Chitosan (CIS) is selected as a carrier for delivering unbound bilirubin (UCB), and the chitosan unbound bilirubin microspheres are prepared through an emulsion reaction and a cross-linking reaction, so that the UCB is more safely and efficiently fed into the intestinal tract to reach an inflammation part, and experiments prove that the UCB can be used for preparing the unbound bilirubin microspheres. The chitosan non-binding bilirubin microsphere has a remarkable protective effect on the intestinal immune function, microbiota disorder and barrier function of a dextran sodium sulfate (DSS) induced ulcerative colitis (UC) mouse.

The invention relates to the technical field of biology, in particular to application of osteopontin in regulation of intestinal anaerobic symbiotic bacteria colonization. The osteopontin is used for preparing food or medicine for improving the immunity of the intestinal tract, and the food or medicine is used for improving the abundance of anaerobic symbiotic bacteria in the intestinal tract; according to the application disclosed by the invention, firstly, by virtue of transcriptome analysis, it is proved that the abundance of Parabacteroids distans and Blautiapseudococcoids can be remarkably improved by virtue of the exogenous osteopontin, so that the abundance of the Parabacteroids distans and the abundance of the Blautiapseudococcoids can be remarkably improved; secondly, the invention also proves that the exogenous osteopontin can be used for inhibiting the proliferation of harmful bacteria Escherichia coli; therefore, the intestinal immunity is improved, the intestinal barrier is improved or repaired, and the intestinal flora balance is maintained. The composition is suitable for infants, old people, patients and other people with low immunity.

The invention discloses breast milk-derived bifidobacterium breve for relieving inflammatory bowel diseases and application of the breast milk-derived bifidobacterium breve. The bifidobacterium breve is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is November 13, 2024, and the preservation number is CGMCC No. 32608. The bifidobacterium breve has the function of regulating the immunity of the intestinal tract, can effectively enhance the barrier of the intestinal tract, and plays a positive role in relieving the symptoms of colitis; compared with a traditional medicine for treating colitis, a medicine or a probiotic preparation prepared from the bifidobacterium breve has certain advantages and has a wide application prospect.

The invention discloses application of a molecular genetic marker in simultaneous prediction of intestinal immunity, egg weight at first laying and body weight at first laying. The molecular genetic marker disclosed by the invention can be used for simultaneously marking the intestinal immune function, the egg weight at the first laying time and the body weight at the first laying time; by means of the detection method, one-time detection can be achieved, and early, accurate and efficient prediction of the intestinal immune function, the first laying weight and the first laying egg weight of the chickens can be completed. The'one-point three-detection 'technology accords with the mainstream goal of laying hen breeding, and can save the detection cost: taking a single sample detection condition as an example, under the detection method of the same type of PCR principle, corresponding sites are respectively detected for three times if the three characters are detected, however, the prediction of the three characters can be realized by only detecting for one time in the invention, and the detection cost is greatly reduced. The detection cost is reduced by 2 / 3, and meanwhile, the important characters of a plurality of chickens can be predicted.