40 results about "Intestinal immunity" patented technology

The invention discloses an enzymolysis preparation method of high-immunocompetence bovine coloctrum peptide, active peptide and application thereof, and belongs to the field of biotechnology and functional foods.The enzymolysis preparation method is characterized by comprising the steps that bovine coloctrum is degreased, trehalose is added into obtained supernate, first stirring is conducted, and first mixed liquid is obtained; wherein the supernate comprises whey protein and Ig; adding medium protease into the first mixed solution for hydrolysis to obtain enzymatic hydrolysate; wherein the enzymatic hydrolysate comprises a mixture of bovine colostrum hydrolyzed peptides, the Ig activity retention rate is larger than or equal to 90% through the targeted protection and directional enzymolysis technology, the beta-lactoglobulin peptide with the synergistic immune enhancement effect is obtained, the Ig and small molecule peptides in the product have the synergistic effect, the body immune regulation capacity and the intestinal immune function are remarkably improved, the immunity of the human body is improved, and the immunity of the human body is improved. The method can be widely applied to the fields of infant formulas, postoperative nutrition, functional drinks and the like.

The invention discloses a method for preparing a high-fiber gel minced fillet product based on low-molecular-weight tremella polysaccharide. The method comprises the following steps: modifying the low-molecular-weight tremella polysaccharide; dissolving the modified low-molecular-weight tremella polysaccharide in water to obtain tremella homogenate; and preparing the minced fillet: mixing fish meat with the modified low-molecular-weight tremella polysaccharide, adding table salt and auxiliary materials, and heating and curing to obtain the high-fiber gel minced fillet product. According to the tremella polysaccharide surimi composite product prepared by the preparation method disclosed by the invention, the modified tremella polysaccharide slurry is added in the surimi processing process, so that the gel performance of surimi can be well improved, the surimi has high fiber property, the smooth and elastic mouth feel of the surimi product can be enhanced, and the taste bud experience is richer. Meanwhile, as the tremella polysaccharide has rich biological activity, such as antioxidation, intestinal immunity regulation, blood sugar and blood fat reduction and the like, the tremella polysaccharide can be added into the surimi product as one of the raw materials, and additional functional characteristics can be endowed to the surimi product.

The application discloses application of Gastrodia elata extract in preparation of a feed additive for resisting Aeromonas hydrophila infection, and belongs to the technical field of water animal disease prevention and treatment. The application first finds that the Gastrodia elata extract has a significant effect of inhibiting Aeromonas hydrophila infection. After the Gastrodia elata extract is added into water animal feed as a feed additive and is fed to the water animal, the Gastrodia elata extract is absorbed into blood circulation system through a digestive tract, up-regulates intestinal immunoglobulin gene expression, improves serum immunoglobulin level, enhances intestinal barrier function, improves animal activity, effectively resists Aeromonas hydrophila infection, reduces disease occurrence, and improves yield and quality of animal breeding. Therefore, the Gastrodia elata extract has a good application prospect.

The invention provides an application of a transcription factor PU.1 inhibitor or a pharmaceutically acceptable salt thereof in preparation of a medicine for treating ulcerative colitis, and belongs to the technical field of biological medicines. According to the invention, intervention is carried out from the upstream of an inflammation network through a small-molecule inhibitor specifically targeting a core transcription factor PU.1, and the multi-dimensional and synergistic treatment effect on ulcerative colitis is realized by regulating and controlling the functions of intestinal immune cells and maintaining the integrity of an intestinal barrier and the steady state of intestinal flora.

This invention discloses a naturally formed nutritional licking brick specifically for musk deer and its preparation method. Using calcium silicate as the skeleton material, it is compounded with yeast culture powder, bentonite, compound probiotics, sweeteners, and flavoring agents. The resulting licking brick continuously releases soluble nutrients and active bacteria during the musk deer's licking process. The specific combination of probiotics and yeast culture powder components effectively improves the intestinal immunity of captive musk deer, significantly reduces intestinal inflammation, and simultaneously lowers cortisol levels in feces, alleviating stress responses. Using yeast culture powder, calcium silicate, and bentonite as a composite coagulant, the licking brick prepared by mold casting exhibits optimal moisture resistance during storage. Furthermore, the licking brick has a smooth appearance, a compact and sturdy structure, excellent molding performance, and retains its structural integrity even after immersion in water, making it suitable for preparing products related to improving the intestinal health and immunity of musk deer.

The invention discloses application of a molecular genetic marker in simultaneous prediction of intestinal immunity, egg weight at first laying and body weight at first laying. The molecular genetic marker disclosed by the invention can be used for simultaneously marking the intestinal immune function, the egg weight at the first laying time and the body weight at the first laying time; by means of the detection method, one-time detection can be achieved, and early, accurate and efficient prediction of the intestinal immune function, the first laying weight and the first laying egg weight of the chickens can be completed. The'one-point three-detection 'technology accords with the mainstream goal of laying hen breeding, and can save the detection cost: taking a single sample detection condition as an example, under the detection method of the same type of PCR principle, corresponding sites are respectively detected for three times if the three characters are detected, however, the prediction of the three characters can be realized by only detecting for one time in the invention, and the detection cost is greatly reduced. The detection cost is reduced by 2 / 3, and meanwhile, the important characters of a plurality of chickens can be predicted.

This invention relates to a strain of *Lactobacillus plantarum* with accession number 35817 from the China General Microbiological Culture Collection Center (CGMCC), and its application in improving intestinal immune function and promoting human intestinal health. This strain can be used as a probiotic ingredient to prepare compositions with the following functions: enhancing resistance to intestinal bacterial infections, maintaining intestinal barrier function, and regulating inflammatory responses. The compositions can be formulated into foods, beverages, or pharmaceuticals for enhancing intestinal immune function and intestinal health.

The present invention relates to the field of probiotics, in particular to an Akkermansia muciniphila MG784 for alleviating constipation, promoting defecation, improving intestinal barrier function and intestinal immunity, and its uses. The Akkermansia muciniphila MG784 provided by the present invention has the deposition number DSM 35549. The Akkermansia muciniphila MG784 provided by the present invention can increase the levels of motilin (MTL), gastrin (GAS), and acetylcholine in the gastrointestinal tract, promote intestinal peristalsis, improve the small intestine propulsion rate, and ameliorate defecation time and fecal output. Furthermore, Akkermansia muciniphila MG784 can also alleviate colonic mucosal inflammation, improve the thickness of the digestive tract muscular layer, modulate the structure of the intestinal microbiota, significantly increase the abundance of beneficial bacteria, and decrease the abundance of harmful bacteria.

This invention belongs to the field of food nutrition and functional factors, specifically relating to a method for preparing highly immunomodulatory ovotransferrin (OVT) hydrolysates based on an adult gastrointestinal simulated digestive system. The method involves processing OVT using a simulated adult gastrointestinal tract to prepare OVT hydrolysates containing HTEGSTT, TTSY, and / or RTAGWVIPMG. This invention reveals that OVT exhibits enhanced immunomodulatory activity after gastrointestinal digestion and hydrolysis, and is more readily hydrolyzed in simulated adult digestive fluids than in infant digestive fluids. Comparative experiments show that nutritional intervention with OVT simulated digestive products from adults and infants can promote the production of short-chain fatty acids such as acetic acid, propionic acid, and butyric acid, effectively alleviate swelling and damage to the small intestinal mucosa caused by inflammation, significantly improve intestinal barrier immune function, and OVT IA exhibits higher intestinal immune activity than OVT IB.

The invention discloses a feed additive for improving pathogen resistance and intestinal injury of high-fat fed fishes and application of the feed additive, and belongs to the technical field of fish feed. The feed additive is indole-3-propionic acid, and the application of the feed additive is that IPA is added into a high-fat feed according to the addition amount of 20-160 mg / kg. Experiments find that IPA with different concentrations can significantly improve the aeromonas hydrophila infection resistance of tilapia fed with high fat, enhance the intestinal immune enzyme activity, improve the intestinal permeability, enhance the intestinal antioxidant ability and relieve intestinal inflammation. The IPA disclosed by the invention can be used as a novel feed additive, can effectively relieve the problems of pathogen resistance reduction and intestinal injury of high-fat fed fishes, and has a good application prospect.

The invention relates to the technical field of biological deep processing and extraction, and discloses a directional preparation method of intestinal immunity-enhanced sea cucumber peptide, which comprises the following steps: (1) sea cucumber pretreatment; (2) carrying out deep penetration wall breaking on the supercritical CO2 / deep eutectic solvent; (3) performing enzymolysis treatment; (4) peptomics analysis; (5) performing molecular docking and virtual screening on the sea cucumber peptide and the TLR2 receptor; (6) membrane separation targeted enrichment; and (7) performing rotary evaporation, concentration and drying to obtain the sea cucumber peptide. Supercritical CO2 with strong permeation and diffusion characteristics is used as a carrier and cooperates with the deep-eutectic solvent to penetrate through a compact cuticle structure of sea cucumbers. The SC-CO2 acts on a stratum corneum lipid phase, a remarkable micropore expansion effect is initiated, and the pore density is greatly improved; functional ions in the deep eutectic solvent accurately destroy a salt bridge cross-linked network of cuticle key structure protein, and solvent components induce deep swelling of collagen through hydrogen-bond interaction, so that effective disintegration of the cuticle structure is realized.

The invention discloses agrocybe cylindracea immunoactive peptide with a function of relieving colitis as well as a preparation method and application thereof, and relates to the technical field of active peptide. The invention provides an agrocybe cylindracea immunocompetent peptide. The amino acid sequence of the agrocybe cylindracea immunocompetent peptide is Leu-Ala-Leu-Trp-Pro. The agrocybe cylindracea immune active peptide can be prepared through artificial chemical synthesis and can also be obtained through separation and purification of agrocybe cylindracea protein enzymatic hydrolysate. The agrocybe cylindracea immunocompetent peptide has an immunoregulation effect of regulating polarization of macrophages, has biological activity of relieving colitis, and can repair an intestinal mucosa barrier function and regulate intestinal immunity so as to relieve colitis.

The invention provides methods for modulating intestinal immune function, modulating inflammatory responses, treating an inflammatory disorder, treating an autoimmune disorder, treating an allergic disorder, or treating a degenerative disorder in an animal by modulating the amount of one or more phosphatidylglycerol compounds in the animal (e.g., by oral administration of a phosphatidylglycerol compound).

The invention provides an animal intestinal immune regulation feed additive and feed, and relates to the technical field of animal feed. The animal intestinal immune regulation feed additive provided by the invention is prepared by drying an additive premixed solution, 100 parts by mass of the additive premix liquid comprise 20-40 parts of a plant source silicon aqueous solution with the mass concentration of 30%-40%, 10-20 parts of fructo-oligosaccharide, 5-10 parts of beta-glucan, 2-5 parts of a tea saponin or plant polyphenol aqueous extract, 10-15 parts of calcium amino acid chelate and the balance of a carrier; the particle size of the plant source silicon element is 0.4 nm. The feed additive is good in stability, high in safety, easy to absorb, suitable for animal feed and health care products, especially suitable for animals in the breeding period and animals sensitive to intestinal tracts, and capable of meeting the requirements of the current animals for efficient absorption type nourishment.

This invention discloses a DC-specific knockout animal model of the Adamdec1 gene, its construction method, and its applications. The method involves inserting Loxp sites at both ends of exons 2–10 of the Adamdec1 gene to obtain the Adamdec1 gene. flox / flox Mice, then with Cd11c cre / + Mouse hybridization and screening to obtain Cd11c cre / + Adamdec1 flox / flox In mice, the Adamdec1 gene was specifically knocked out in dendritic cells. This model, induced by 3% DSS, exhibited a more severe colitis phenotype and can be used to establish a stable and reliable research model for acute colitis. This invention provides a precise tool for studying the function of Adamdec1 in intestinal immune regulation and has significant scientific and translational value.

This invention relates to the fields of biomedicine and functional food technology, and discloses an intestinal immune regulation system based on the microenvironmental release of black goji berry polysaccharides (LBPs). The system includes a microenvironment-responsive delivery unit for delivering and releasing LBPs. The delivery unit comprises a LBP core and a responsive layer encapsulating the core. The responsive layer contains at least one responsive material capable of responding to microenvironmental signals at the intestinal target site, enabling the delivery unit to undergo structural changes in response to these signals upon reaching the intestinal target site, thereby achieving the targeted release of the LBPs. This intestinal immune regulation system based on the microenvironmental release of LBPs constructs a physical barrier by encapsulating the active core of LBPs with a "responsive layer" composed of responsive materials. This system can effectively resist the damage to the LBP structure caused by the harsh environment of the upper digestive tract.

The invention relates to the field of biological medicine, and discloses a composition targeting a lactobacillus johnsonii-tryptophan metabolic axis and application of the composition. The composition comprises a traditional Chinese medicine extract and lactobacillus johnsonii, the traditional Chinese medicine extract is prepared from radix bupleuri, radix paeoniae alba, fructus aurantii immaturus and honey-fried licorice root. Traditional Chinese medicine extracts of radix bupleuri, radix paeoniae alba, fructus aurantii immaturus and honey-fried licorice root are compounded with lactobacillus johnsonii according to a specific proportion, tryptophan is promoted to be converted into 5-hydroxytryptamine, kynurenine is inhibited from being generated, depression-like behaviors are effectively relieved, the traditional Chinese medicine composition can cooperate with anticancer drugs to remarkably inhibit cancer cell growth, meanwhile, the intestinal barrier function can be improved, the intestinal immunity can be improved, and the curative effect is good. The invention provides a high-safety synergistic treatment scheme with triple effects of tumor inhibition, depression resistance and intestinal tract improvement for patients with liver cancer and depression.

This invention relates to the preparation and application of a chitin-membrane-deficient zebrafish strain. The invention obtains a zebrafish strain with a completely absent intestinal chitin membrane by knocking out the chs1 gene. The nucleotide sequence of the CRISPR / Cas9-based sgRNA of the knocked-out zebrafish chs1 gene is shown in SEQ ID NO: 18, and the nucleotide sequence targeting the chs1 gene is shown in SEQ ID NO: 1. The chitin-membrane-deficient zebrafish strain of this invention can be used for research on intestinal chitin membranes, intestinal digestion and absorption, intestinal flora, intestinal epithelial renewal and aging, and intestinal immune function. It can also be used to study the mechanisms of damage to the intestine caused by harmful substances, including but not limited to pathogenic microorganisms, microplastics, and heavy metals. Furthermore, it can serve as an ideal small intestinal model for studying mammalian small intestinal diseases.

The invention discloses a nutritional preparation suitable for people with deficiency of five internal organs to improve immunity and resist oxidation, and particularly relates to the field of pharmaceutical preparations. A two-phase micro-capsule coating system is adopted, and the capsule is formed by physically mixing a gastric-soluble coating micro-capsule and an enteric-coated and colon-targeted double-layer coating micro-capsule according to the weight ratio of 3: 7. The gastric coating adopts gastric-soluble acrylic resin, the enteric layer adopts enteric acrylic resin, the colon targeting layer adopts a pectin / chitosan compound, and a fluidized bed coating process is adopted for segmented preparation. About 30% of active ingredients are quickly released in the stomach to play a role in soothing the nerves, and the rest 70% of the active ingredients are precisely and slowly released from the intestinal tract to the colon to act on the immune system of the intestinal tract. The problems that active ingredients in a traditional preparation are prone to inactivation in a gastric acid environment, the bioavailability is low, targeting is lacked, and the storage stability is poor are effectively solved.

The invention provides a compound probiotic composition and application thereof in a product for improving intestinal health, the compound probiotic composition is prepared from lactobacillus rhamnosus GG, lactic acid bacteria compound powder, plant lactobacillus GOLDGUT-LP1024 and lactobacillus paracasei GOLDGUT-LC12345, and the lactobacillus rhamnosus GG, the lactic acid bacteria compound powder, the plant lactobacillus GOLDGUT-LC12345 and the lactobacillus paracasei GOLDGUT-LC12345 are added into the compound probiotic composition. The lactic acid bacteria compound powder is prepared by mixing bifidobacterium longum subsp. Longum BORI, lactobacillus acidophilus AD031, bifidobacterium bifidum BGN4, bifidobacterium animalis subsp. Lactis AD011 and bifidobacterium longum subsp. Infantis IBS007; the plant lactobacillus GOLDGUT-LP1024 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the plant lactobacillus GOLDGUT-LP1024 is CGMCC No.28801. The compound probiotic composition provided by the invention can improve the immune function and metabolism function of the intestinal tract so as to improve the health of the intestinal tract and relieve antibiotic-related diarrhea.

The invention discloses a nano preparation for treating inflammatory bowel disease as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The nano preparation comprises a cerium dioxide nano point modified by a CD25 antibody, the nano preparation can target regulatory T cells and remove active oxygen in the regulatory T cells so as to reverse aging of the regulatory T cells. The nano preparation provided by the invention is composed of the CD25 antibody modified cerium dioxide nano dots, can specifically target regulatory T cells, and recovers the immunosuppression function of the cells and the immune homeostasis of intestinal tracts by removing excessive active oxygen in the regulatory T cells and reversing cell senescence.

The application of recombinant ultra-high molecular weight hyaluronic acid in regulating intestinal immunity in colitis belongs to the field of biomedical technology. This invention addresses the limitations of existing treatments for inflammatory bowel disease (IBD), which suffer from limited efficacy, significant side effects, and difficulty in targeting and repairing the intestinal immune barrier. It provides the application of recombinant ultra-high molecular weight hyaluronic acid in regulating intestinal immunity in colitis. This recombinant ultra-high molecular weight hyaluronic acid can activate the intestinal IgA-producing immune network, regulate inflammation-related genes, and repair the physical and immune barriers of the colonic mucosa, thus exhibiting a superior protective effect against colitis compared to conventional hyaluronic acid, providing a new candidate drug for the treatment of IBD.

The invention discloses an application of chrysin-7-O-beta-glucuronic acid in improvement of functions of intestines and stomach. The chrysin-7-O-beta-glucuronic acid is derived from a natural food material with homology of medicine and food; the chrysin-7-O-beta-glucuronic acid can improve the intestinal microenvironment, relieve the oxidative stress and inflammatory response of intestinal mucosa, promote the integrity recovery of intestinal epithelial barrier, and cooperatively regulate the immune homeostasis of the intestinal tract, so that the chrysin-7-O-beta-glucuronic acid is helpful for recovering the normal permeability and peristalsis function of the intestinal tract, and can be used for preventing and treating intestinal diseases. The invention provides an intestinal function improvement scheme which is safer, can be applied for a long time and is easier to accept.

This invention discloses a method for preparing lactoferrin-polyphenol nanocomposites with intestinal immune function. The method involves the following steps: First, lactoferrin is moderately hydrolyzed under the action of a complex protease to optimize its molecular structure. Then, it is compounded with water-soluble polyphenols (such as chlorogenic acid or epicatechin) using metal ion chelation technology, and Ca²⁺ is used to induce lactoferrin bridging and aggregation to form a complex. Next, ultrasonic homogenization is performed to further control the particle size, ensuring it is distributed within the range of 100-300 nm. The resulting complex is then concentrated by nanofiltration and electrostatic spray drying to obtain a structurally stable shell-core nanocomposite, which is then nitrogen-filled to prevent oxidation. The lactoferrin-polyphenol nanocomposite prepared by this invention not only exhibits good stability and intestinal immune-enhancing effects but also significant antibacterial activity, making it widely applicable in functional foods and nutritional supplements.

This invention relates to *Bifidobacterium adolescentis*, a composition containing it, and its application in preparing intestinal inflammation models, belonging to the field of molecular biology. To address the lack of existing research on the role of *Bifidobacterium adolescentis* and phenylalanine in early life immune regulation, this invention provides *Bifidobacterium adolescentis* SZBAD1096 (accession number GDMCC NO:67848), comprehensively revealing the molecular mechanism by which *Bifidobacterium adolescentis* SZBAD1096 mediates host intestinal immune damage through the phenylalanine metabolic pathway, and its application in preparing intestinal inflammation models. This provides important theoretical and experimental basis for accurately assessing the safety of *Bifidobacterium* strains and developing inflammatory intervention strategies targeting the intestinal microecology.

The invention provides an application of fructus psoraleae polysaccharide in preparation of a medicine or health food for regulating an intestinal barrier function. Specifically, the fructus psoraleae polysaccharide acts on an intestinal tract. In the intestinal tract action process, the fructus psoraleae polysaccharide effectively improves damaged intestinal tract barrier repair; the number of CD4 + and CD8 + T cells in intestinal tracts is increased, and the immune barrier of the intestinal tracts is improved; according to the present invention, the expression of tight junction proteins Claudin-1, Occludin and ZO-1 can be promoted, the intestinal mechanical barrier can be repaired and enhanced, and the polypeptide can be used for preventing, relieving or treating intestinal barrier damage, has excellent performance in the regulation of the intestinal barrier function, and has good application prospects.

The present invention relates to food allergen compositions. In particular, the present invention relates to novel compositions and methods for the diagnosis or treatment of food allergies. Specifically, the present invention discloses a novel method of delivering food allergens to an allergic patient by oral administration of a formulation that dissolves and releases the proteins in the stomach. The present invention allows for the treatment of food allergies by delivering food allergens to the intestinal immune system and controlled exposure to the esophagus or oral cavity. The present invention also allows for the assessment of the threshold of clinical reactivity by food provocation without exposure to the esophagus and oral cavity. The present invention can be used in any subject, particularly a human subject, and is applicable to any food allergen.

The invention discloses an infant nutritional supplement and a preparation method thereof, and belongs to the technical field of nutritional preparations. The Korean ginseng, the codonopsis pilosula and the coix seed extract are mixed according to a specific mass ratio, and the optimal synergistic balance of the mild regulation effect on the intestinal smooth muscle of the infants, the enhancement effect on the intestinal immune barrier and the promotion effect on digestive enzymes can be achieved, so that the absorption of the infants on nutrient substances is promoted. Small molecule peptides (whey protein hydrolysis peptides and casein phosphopeptides) which are easier to digest and absorb are selected, so that the digestion burden of infants is reduced, and the small molecule peptides are highly consistent with the targets of conditioning the intestinal function and improving the absorption efficiency of a'micro-capsule system '.

The invention provides a composition for improving survival, growth and intestinal health of larimichthys crocea juveniles, a feed additive and application of the composition and the feed additive, and belongs to the technical field of aquatic feed and feed additives. The composition provided by the invention consists of propionic acid and vitamin B12. Propionic acid can improve expression of tight junction protein of intestinal tracts, promote growth of intestinal epithelial cells, strengthen physical barrier functions of the intestinal tracts, regulate absorption of nutrient substances by the intestinal tracts, inhibit expression of proinflammatory factors of the intestinal tracts and strengthen immune barrier functions of the intestinal tracts. Vitamin B12 can promote propionic acid to play a beneficial role in a body and regulate oxidative stress response and inflammatory response. Through the synergistic effect of the propionic acid and the vitamin B12, the immunocompetence of the juvenile fish can be effectively enhanced, and the survival rate and the growth performance of the juvenile fish are improved. In addition, the propionic acid and the vitamin B12 are low in raw material cost, high in economical efficiency and high in operability.

A kind of granzyme B targeting complex, radiopharmaceutical and its imaging application in intestinal immune abnormal activity belong to the field of nuclear medicine diagnosis and treatment.The complex is covalently modified, specifically non-covalently combined with granzyme B by recognizing peptide first, and then triggers irreversible covalent connection by means of proximity effect, effectively solves the technical bottleneck of low target tissue uptake, short in vivo retention time and narrow imaging window of existing granzyme B probe, significantly improves the selective retention capacity and imaging specificity of the probe in the target site.The complex and radiopharmaceutical of the present application can specifically target granzyme B released by intestinal lesion site immune abnormal activity, realize non-invasive, dynamic, quantitative nuclear medical imaging of intestinal inflammation such as Crohn's disease, ulcerative colitis and aGVHD, and accurately evaluate the degree of intestinal immune abnormal activity and monitor the treatment effect, providing a core tool for early diagnosis and precise diagnosis and treatment of such diseases, and having important clinical transformation value and application prospect.