66 results about "Intestinal immunity" patented technology

The invention provides an Akkermann Amuc1100 protein mutant as well as a preparation method and application thereof, wild Akkermann Amuc1100 protein is subjected to mutation modification through a molecular biological technical means, and the bioactivity of the Amuc1100 protein mutant subjected to molecular modification is obviously improved compared with that of the wild protein; compared with a wild type Amuc1100 protein, the wild type Amuc1100 protein has a better effect when being applied to the aspects of regulating intestinal immunity, relieving acute pancreatitis, promoting browning of white adipose cells, inhibiting obesity, relieving ulcerative colitis and the like, and a very good application prospect is provided for developing bioactive medicines with higher efficiency.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

The invention discloses an enzymolysis preparation method of high-immunocompetence bovine coloctrum peptide, active peptide and application thereof, and belongs to the field of biotechnology and functional foods.The enzymolysis preparation method is characterized by comprising the steps that bovine coloctrum is degreased, trehalose is added into obtained supernate, first stirring is conducted, and first mixed liquid is obtained; wherein the supernate comprises whey protein and Ig; adding medium protease into the first mixed solution for hydrolysis to obtain enzymatic hydrolysate; wherein the enzymatic hydrolysate comprises a mixture of bovine colostrum hydrolyzed peptides, the Ig activity retention rate is larger than or equal to 90% through the targeted protection and directional enzymolysis technology, the beta-lactoglobulin peptide with the synergistic immune enhancement effect is obtained, the Ig and small molecule peptides in the product have the synergistic effect, the body immune regulation capacity and the intestinal immune function are remarkably improved, the immunity of the human body is improved, and the immunity of the human body is improved. The method can be widely applied to the fields of infant formulas, postoperative nutrition, functional drinks and the like.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention belongs to the field of food nutrition and functional factors, and particularly relates to a method for preparing ovotransferrin hydrolysate with high immunocompetence based on an adult gastrointestinal simulated digestion system. According to the method, ovotransferrin is treated through the stomach and intestine of a simulated adult, OVT hydrolysate is prepared, and the OVT hydrolysate contains HTEGSTT, TTSY and / or RTAGWVIPMG. The invention finds that the immunomodulatory activity of OVT is enhanced after gastrointestinal digestion and hydrolysis, the OVT is more easily hydrolyzed in simulated adult digestive juice than in infant digestive juice, and a contrast experiment shows that the nutrition intervention of OVT adult and infant simulated digestion products can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid; the swelling injury caused by inflammation to small intestine mucous membrane tissues is effectively relieved, so that the intestinal barrier immune function is obviously improved, and the OIA shows higher intestinal immune activity than the OIB.

The invention provides a composition for promoting differentiation of intestinal immune cells as well as a preparation method and application of the composition. According to the composition, 1, 3-dioleic acid-2-palmitic acid triglyceride and breast milk oligosaccharide are used in a combined manner, so that monocytes can be effectively promoted to differentiate into intestinal macrophages, and inflammation is reduced to a certain extent. After the monocytes are differentiated into the intestinal macrophages, the intestinal immune homeostasis and intestinal microbiota inflammatory response can be regulated, so that the mucosal immune homeostasis and intestinal epithelial cell barrier function are maintained. Therefore, the composition provided by the invention can improve the intestinal immune barrier by promoting the monocytes to differentiate into the intestinal macrophages, and provides a new thought for improving the early intestinal barrier of infants through nutritional supplement.

The present application relates to a kind of composition for adjusting intestinal flora and its preparation method and application, and the raw materials of the composition include: fried atractylodes, dried tangerine or orange peel, fried liquorice, magnolia officinalis (ginger preparation), polyporus umbellatus, poria cocos (skin removal), fried atractylodes, alisma orientalis, cassia bark, dried ginger, dark plum.The present application produces the effect of anti-inflammatory, antioxidant, regulating intestinal flora, improving intestinal immunity and intestinal barrier protection effect etc. through the synergistic effect between raw materials.

The invention relates to livestock feeding probiotics, in particular to bacillus subtilis and application, the bacillus subtilis is bacillus subtilis BW and is preserved in the China General Microbiological Culture Collection Center on April 10, 2025, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) 0.34165. The bacillus subtilis BW disclosed by the invention can be colonized in animal bodies, so that the diarrhea rate of piglets is remarkably reduced, the immunity of intestinal tracts is improved, and the health of the intestinal tracts is improved; after the bacillus subtilis BW is used for feeding ruminants, the feed conversion efficiency can be remarkably improved, the growth performance can be improved, and the bacillus subtilis BW has a good application prospect in livestock and poultry breeding.

The invention relates to the technical field of chemical toxicology, in particular to an evaluation method for comprehensively analyzing the immune induction effect of a plasticizer by using caenorhabditis elegans, according to the evaluation method, a mother worm breeding mode is changed to replace a lysis solution to synchronize nematodes, experimental interference is reduced, the result accuracy is improved, monitoring indexes are newly increased, intestinal injuries and in-vivo bacterial indexes are increased, and the evaluation method is suitable for comprehensive analysis of the immune induction effect of the plasticizer. The evaluation is more accurate and reliable by combining oxidative stress indexes and performing two-dimensional comprehensive evaluation from an intestinal immune defense line and oxidative stress, and by drawing a multi-index monitoring shape graph, adopting a comprehensive biomarker response index analysis method and associating immune-related representative indexes, the evaluation accuracy is improved. According to the method, the change of each index and the immunity intensity on the caenorhabditis elegans under different exposure conditions are intuitively presented, the immune defense effect of the caenorhabditis elegans under the stress of the plasticizer can be quickly and effectively evaluated without transcriptome and metabonomics, and efficient evaluation is realized.

The invention discloses a method for preparing a high-fiber gel minced fillet product based on low-molecular-weight tremella polysaccharide. The method comprises the following steps: modifying the low-molecular-weight tremella polysaccharide; dissolving the modified low-molecular-weight tremella polysaccharide in water to obtain tremella homogenate; and preparing the minced fillet: mixing fish meat with the modified low-molecular-weight tremella polysaccharide, adding table salt and auxiliary materials, and heating and curing to obtain the high-fiber gel minced fillet product. According to the tremella polysaccharide surimi composite product prepared by the preparation method disclosed by the invention, the modified tremella polysaccharide slurry is added in the surimi processing process, so that the gel performance of surimi can be well improved, the surimi has high fiber property, the smooth and elastic mouth feel of the surimi product can be enhanced, and the taste bud experience is richer. Meanwhile, as the tremella polysaccharide has rich biological activity, such as antioxidation, intestinal immunity regulation, blood sugar and blood fat reduction and the like, the tremella polysaccharide can be added into the surimi product as one of the raw materials, and additional functional characteristics can be endowed to the surimi product.

The application belongs to the technical field of biological nanomaterials, and particularly relates to an immune activation nanosheet as well as a preparation method and application thereof. The immune activation nanosheet comprises a two-dimensional montmorillonite nanosheet with a sheet layer structure, and manganese ions and tumor antigens loaded in the interlayer of the two-dimensional montmorillonite nanosheet. The mass ratio of the manganese ions to the montmorillonite is 1:5-1:20, and the mass ratio of the tumor antigens to the montmorillonite is 1:1-1:10. The immune activation nanosheet has the characteristics of good oral stability, high mucosal targeting, strong immune efficacy, etc. The stability of the immune activation nanosheet in the gastrointestinal tract is good, the immune activation nanosheet can be effectively enriched in the small intestine, a vaccine inoculation pool is formed, the intestinal immune tolerance can be overcome, the immune effect is enhanced, and the immune activation nanosheet has a clinical application prospect.

The invention discloses a probiotic composition, fermented yoghurt and application of the probiotic composition and the fermented yoghurt in improving related indexes of athletes. The probiotic composition comprises bacillus subtilis SP-8-5, lactobacillus plantarum YM-4-3, lactobacillus plantarum AY01 and pediococcus acidilactici LH01. The composition of intestinal microbial populations can be adjusted through a specific compounding method, the abundance of harmful bacteria is reduced, the abundance of beneficial bacteria is improved, the immunity of intestinal tracts is enhanced, and the integrity of intestinal barriers of athletes is maintained. Meanwhile, the composition has a positive improvement effect on related exercise indexes such as plasma amino acid, excrement metabolites and exercise performance, and can be used as an effective exercise supplement to be applied to athletes.

The application discloses application of Gastrodia elata extract in preparation of a feed additive for resisting Aeromonas hydrophila infection, and belongs to the technical field of water animal disease prevention and treatment. The application first finds that the Gastrodia elata extract has a significant effect of inhibiting Aeromonas hydrophila infection. After the Gastrodia elata extract is added into water animal feed as a feed additive and is fed to the water animal, the Gastrodia elata extract is absorbed into blood circulation system through a digestive tract, up-regulates intestinal immunoglobulin gene expression, improves serum immunoglobulin level, enhances intestinal barrier function, improves animal activity, effectively resists Aeromonas hydrophila infection, reduces disease occurrence, and improves yield and quality of animal breeding. Therefore, the Gastrodia elata extract has a good application prospect.

The invention provides an application of a transcription factor PU.1 inhibitor or a pharmaceutically acceptable salt thereof in preparation of a medicine for treating ulcerative colitis, and belongs to the technical field of biological medicines. According to the invention, intervention is carried out from the upstream of an inflammation network through a small-molecule inhibitor specifically targeting a core transcription factor PU.1, and the multi-dimensional and synergistic treatment effect on ulcerative colitis is realized by regulating and controlling the functions of intestinal immune cells and maintaining the integrity of an intestinal barrier and the steady state of intestinal flora.

This invention discloses a naturally formed nutritional licking brick specifically for musk deer and its preparation method. Using calcium silicate as the skeleton material, it is compounded with yeast culture powder, bentonite, compound probiotics, sweeteners, and flavoring agents. The resulting licking brick continuously releases soluble nutrients and active bacteria during the musk deer's licking process. The specific combination of probiotics and yeast culture powder components effectively improves the intestinal immunity of captive musk deer, significantly reduces intestinal inflammation, and simultaneously lowers cortisol levels in feces, alleviating stress responses. Using yeast culture powder, calcium silicate, and bentonite as a composite coagulant, the licking brick prepared by mold casting exhibits optimal moisture resistance during storage. Furthermore, the licking brick has a smooth appearance, a compact and sturdy structure, excellent molding performance, and retains its structural integrity even after immersion in water, making it suitable for preparing products related to improving the intestinal health and immunity of musk deer.

The invention discloses a chitosan non-binding bilirubin microsphere as well as a preparation method and application thereof. The chitosan non-binding bilirubin microspheres are prepared by taking chitosan as a delivery carrier and taking chitosan and bilirubin as main raw materials through an emulsion reaction and a cross-linking reaction in sequence. Chitosan (CIS) is selected as a carrier for delivering unbound bilirubin (UCB), and the chitosan unbound bilirubin microspheres are prepared through an emulsion reaction and a cross-linking reaction, so that the UCB is more safely and efficiently fed into the intestinal tract to reach an inflammation part, and experiments prove that the UCB can be used for preparing the unbound bilirubin microspheres. The chitosan non-binding bilirubin microsphere has a remarkable protective effect on the intestinal immune function, microbiota disorder and barrier function of a dextran sodium sulfate (DSS) induced ulcerative colitis (UC) mouse.

The invention discloses application of a molecular genetic marker in simultaneous prediction of intestinal immunity, egg weight at first laying and body weight at first laying. The molecular genetic marker disclosed by the invention can be used for simultaneously marking the intestinal immune function, the egg weight at the first laying time and the body weight at the first laying time; by means of the detection method, one-time detection can be achieved, and early, accurate and efficient prediction of the intestinal immune function, the first laying weight and the first laying egg weight of the chickens can be completed. The'one-point three-detection 'technology accords with the mainstream goal of laying hen breeding, and can save the detection cost: taking a single sample detection condition as an example, under the detection method of the same type of PCR principle, corresponding sites are respectively detected for three times if the three characters are detected, however, the prediction of the three characters can be realized by only detecting for one time in the invention, and the detection cost is greatly reduced. The detection cost is reduced by 2 / 3, and meanwhile, the important characters of a plurality of chickens can be predicted.

This invention relates to a strain of *Lactobacillus plantarum* with accession number 35817 from the China General Microbiological Culture Collection Center (CGMCC), and its application in improving intestinal immune function and promoting human intestinal health. This strain can be used as a probiotic ingredient to prepare compositions with the following functions: enhancing resistance to intestinal bacterial infections, maintaining intestinal barrier function, and regulating inflammatory responses. The compositions can be formulated into foods, beverages, or pharmaceuticals for enhancing intestinal immune function and intestinal health.

The present invention relates to the field of probiotics, in particular to an Akkermansia muciniphila MG784 for alleviating constipation, promoting defecation, improving intestinal barrier function and intestinal immunity, and its uses. The Akkermansia muciniphila MG784 provided by the present invention has the deposition number DSM 35549. The Akkermansia muciniphila MG784 provided by the present invention can increase the levels of motilin (MTL), gastrin (GAS), and acetylcholine in the gastrointestinal tract, promote intestinal peristalsis, improve the small intestine propulsion rate, and ameliorate defecation time and fecal output. Furthermore, Akkermansia muciniphila MG784 can also alleviate colonic mucosal inflammation, improve the thickness of the digestive tract muscular layer, modulate the structure of the intestinal microbiota, significantly increase the abundance of beneficial bacteria, and decrease the abundance of harmful bacteria.

This invention belongs to the field of food nutrition and functional factors, specifically relating to a method for preparing highly immunomodulatory ovotransferrin (OVT) hydrolysates based on an adult gastrointestinal simulated digestive system. The method involves processing OVT using a simulated adult gastrointestinal tract to prepare OVT hydrolysates containing HTEGSTT, TTSY, and / or RTAGWVIPMG. This invention reveals that OVT exhibits enhanced immunomodulatory activity after gastrointestinal digestion and hydrolysis, and is more readily hydrolyzed in simulated adult digestive fluids than in infant digestive fluids. Comparative experiments show that nutritional intervention with OVT simulated digestive products from adults and infants can promote the production of short-chain fatty acids such as acetic acid, propionic acid, and butyric acid, effectively alleviate swelling and damage to the small intestinal mucosa caused by inflammation, significantly improve intestinal barrier immune function, and OVT IA exhibits higher intestinal immune activity than OVT IB.

The invention discloses a feed additive for improving pathogen resistance and intestinal injury of high-fat fed fishes and application of the feed additive, and belongs to the technical field of fish feed. The feed additive is indole-3-propionic acid, and the application of the feed additive is that IPA is added into a high-fat feed according to the addition amount of 20-160 mg / kg. Experiments find that IPA with different concentrations can significantly improve the aeromonas hydrophila infection resistance of tilapia fed with high fat, enhance the intestinal immune enzyme activity, improve the intestinal permeability, enhance the intestinal antioxidant ability and relieve intestinal inflammation. The IPA disclosed by the invention can be used as a novel feed additive, can effectively relieve the problems of pathogen resistance reduction and intestinal injury of high-fat fed fishes, and has a good application prospect.

The invention belongs to the technical field of biology, and discloses a feed additive which is composed of ginseng stems and leaves and clostridium butyricum. The feed additive is used for being added into basal feed. According to the feed additive, 0.5-2 g of ginseng stems and leaves and 1 * 10 < 8 > cfu to 10 * 10 < 8 > cfu of clostridium butyricum are provided for each kilogram of basal feed. According to the biological feed containing the ginseng stems and leaves, the ginseng stems and leaves are combined with clostridium butyricum, and through scientific compatibility and a dual regulation mechanism of system immunity-intestinal immunity, the overall immune level of organisms is improved while intestinal health is guaranteed, so that the production performance and disease resistance of animals are improved. Breeding experiments prove that the dual regulation and control mechanism of'system immunity-intestinal immunity 'is one of important ways for improving the breeding efficiency and the production level of the live pigs. Meanwhile, the invention further provides application of the additive.

The invention relates to the technical field of biological deep processing and extraction, and discloses a directional preparation method of intestinal immunity-enhanced sea cucumber peptide, which comprises the following steps: (1) sea cucumber pretreatment; (2) carrying out deep penetration wall breaking on the supercritical CO2 / deep eutectic solvent; (3) performing enzymolysis treatment; (4) peptomics analysis; (5) performing molecular docking and virtual screening on the sea cucumber peptide and the TLR2 receptor; (6) membrane separation targeted enrichment; and (7) performing rotary evaporation, concentration and drying to obtain the sea cucumber peptide. Supercritical CO2 with strong permeation and diffusion characteristics is used as a carrier and cooperates with the deep-eutectic solvent to penetrate through a compact cuticle structure of sea cucumbers. The SC-CO2 acts on a stratum corneum lipid phase, a remarkable micropore expansion effect is initiated, and the pore density is greatly improved; functional ions in the deep eutectic solvent accurately destroy a salt bridge cross-linked network of cuticle key structure protein, and solvent components induce deep swelling of collagen through hydrogen-bond interaction, so that effective disintegration of the cuticle structure is realized.

The invention belongs to the technical field of microorganisms, and particularly discloses bifidobacterium bifidum FL228.1 and application thereof. In particular to bifidobacterium bifidum: FL228.1. The bifidobacterium bifidum is preserved in China Center for Type Culture Collection on January 29th, 2024, the preservation address is Wuhan University, Wuhan, Hubei province, and the preservation number is CCTCC No.M 2024274. The invention further discloses a preparation method of the bifidobacterium bifidum. The invention discloses bifidobacterium bifidum FL228.1 and application thereof. The bifidobacterium bifidum FL228.1 is capable of effectively promoting the generation of IgA (Immunoglobulin A) in small intestines, enhancing the immune defense of the intestinal tracts and remarkably improving the intestinal injury caused by ETEC (Enterotoxin) infection.

The invention discloses a probiotic composition, a bean-based fermented food and application of the bean-based fermented food. The probiotic composition is prepared from bacillus subtilis SP-8-5, lactobacillus plantarum YM-4-3, lactobacillus plantarum AY01 and pediococcus acidilactici LH01. The fermented soya bean has the advantages that the prepared fermented soya bean can regulate the composition of intestinal microbial populations, reduce the abundance of harmful bacteria, improve the abundance of beneficial bacteria, enhance the immunity of intestinal tracts and maintain the integrity of intestinal barriers of people with exercise requirements through a specific compounding method. Meanwhile, plasma serine can be reduced, cell damage can be reduced, sleep quality can be improved, and the health-care product can be used as an effective exercise supplement to be applied to people with exercise requirements.

The invention provides a culture method for improving intestinal immunity of trachinotus ovatus, which comprises the following steps: selecting a proper sea area, and laying a degradable microbial agent carrier containing phosphate solubilizing bacteria, potassium bacteria and nitrogen-fixing bacteria at the bottom of a net cage to optimize a culture environment; the fish fries are soaked and pretreated with a solution containing beta-glucan and the like before being put in, and the intestinal immune function of the fish fries is activated; staged bait strengthening is adopted in the breeding process, and specific formula bait is fed according to different day ages; the net cage is replaced and washed every 15-20 days, acousto-optic-water flow synergistic immunostimulation is carried out, natural ecological environment changes are simulated, and the stress capacity of the trachinotus ovatus is enhanced. According to the method disclosed by the invention, the intestinal immunity of the trachinotus ovatus is effectively enhanced, the survival rate is increased, the growth is promoted, the feed coefficient is reduced and a new way is provided for healthy culture of the trachinotus ovatus from a multi-dimensional synergistic effect of environment, nutrition and management.

The invention discloses agrocybe cylindracea immunoactive peptide with a function of relieving colitis as well as a preparation method and application thereof, and relates to the technical field of active peptide. The invention provides an agrocybe cylindracea immunocompetent peptide. The amino acid sequence of the agrocybe cylindracea immunocompetent peptide is Leu-Ala-Leu-Trp-Pro. The agrocybe cylindracea immune active peptide can be prepared through artificial chemical synthesis and can also be obtained through separation and purification of agrocybe cylindracea protein enzymatic hydrolysate. The agrocybe cylindracea immunocompetent peptide has an immunoregulation effect of regulating polarization of macrophages, has biological activity of relieving colitis, and can repair an intestinal mucosa barrier function and regulate intestinal immunity so as to relieve colitis.

The application provides a preparation method and application of a polygonatum cyrtonema acid polysaccharide, and belongs to the technical field of polygonatum cyrtonema polysaccharide extraction. The application adopts a separation and purification method of anion exchange chromatography combined with gel chromatography, and finally obtains a polygonatum cyrtonema acid uniform polysaccharide (PCPA) with high purity. In-vivo pharmacodynamic experiments show that the prepared PCPA can significantly relieve intestinal mucosa injury and has high intestinal immune regulation activity. The preparation method provided by the application is stable in process, the prepared polygonatum cyrtonema acid polysaccharide is high in purity, is a uniform polysaccharide, and has an intestinal immune regulation effect.

The invention provides methods for modulating intestinal immune function, modulating inflammatory responses, treating an inflammatory disorder, treating an autoimmune disorder, treating an allergic disorder, or treating a degenerative disorder in an animal by modulating the amount of one or more phosphatidylglycerol compounds in the animal (e.g., by oral administration of a phosphatidylglycerol compound).

The invention provides an animal intestinal immune regulation feed additive and feed, and relates to the technical field of animal feed. The animal intestinal immune regulation feed additive provided by the invention is prepared by drying an additive premixed solution, 100 parts by mass of the additive premix liquid comprise 20-40 parts of a plant source silicon aqueous solution with the mass concentration of 30%-40%, 10-20 parts of fructo-oligosaccharide, 5-10 parts of beta-glucan, 2-5 parts of a tea saponin or plant polyphenol aqueous extract, 10-15 parts of calcium amino acid chelate and the balance of a carrier; the particle size of the plant source silicon element is 0.4 nm. The feed additive is good in stability, high in safety, easy to absorb, suitable for animal feed and health care products, especially suitable for animals in the breeding period and animals sensitive to intestinal tracts, and capable of meeting the requirements of the current animals for efficient absorption type nourishment.