200 results about "Intestinal immunity" patented technology

The invention relates to bifidobacterium animalis subsp. Lactis C-2 with an effect of improving inflammatory bowel diseases and application of the bifidobacterium animalis subsp. Lactis C-2. The bifidobacterium animalis subsp. Lactis C-2 is classified and named as Bifidobacterium animalis subsp. Lactis, and the preservation number of the bifidobacterium animalis subsp. Lactis C-2 is GDMCC No: 65455. The bacterial strain can inhibit the colonization of harmful bacteria and promote the colonization of beneficial bacteria and optimize the composition of intestinal microorganisms by regulating the intestinal microecology, repairing the intestinal mechanical barrier, improving the intestinal immune response, inhibiting oxidative stress reaction and other action mechanisms, thereby inducing anti-inflammatory response and improving the intestinal barrier function. Therefore, the bifidobacterium animalis subsp. Lactis C-2 strain is suitable for preparing the medicine with the effect of preventing, improving or treating the inflammatory bowel disease.

The invention relates to the field of microorganisms, in particular to Akkermansia muciniphila YG2645 with an intestinal tract-immune-metabolic axis regulating function, an inactivated thallus, an extract, a culture, a culture supernatant, a fermentation product and / or a fermentation supernatant of the Akkermansia muciniphila YG2645, a composition containing the Akkermansia muciniphila YG2645 and a preparation method of the composition containing the Akkermansia muciniphila YG2645 and the composition containing the Akkermansia muciniphila YG2645 and the composition containing the composition. The invention also relates to application of the compound in preparation of products for preventing and / or treating inflammatory diseases. The Akkermansia muciniphila YG2645 and related products of the Akkermansia muciniphila YG2645 have the functions of regulating and controlling immune response, regulating and controlling metabolism, regulating intestinal flora and the like, and the Akkermansia muciniphila YG2645 and the related products of the Akkermansia muciniphila YG2645 have remarkable application value and market potential.

The invention discloses construction and application of an oral gel drug delivery system based on a yeast microcapsule, and belongs to the field of new auxiliary materials and new dosage forms of pharmaceutical preparations, the drug delivery system is composed of budesonide nanoparticles, the yeast microcapsule and gel, budesonide nanoparticles are prepared from budesonide and a polylactic acid-glycolic acid copolymer through a water-in-oil emulsification evaporation technology, yeast cell wall microcapsules are formed after content of yeast powder is removed, and the budesonide-loaded yeast microcapsules are prepared from the yeast microcapsules and the budesonide nanoparticles through stirring, ultrasonic treatment and freeze drying methods. The yeast cell wall microcapsule without contents is used as a carrier, so that the medicine is protected from being degraded by gastric acid, and the intestinal immune response is regulated by targeting intestinal related lymphatic tissues (GALT). The gel prepared by combining pectin can prolong the residence time of the drug in the intestinal tract, realizes continuous release, reduces the whole-body side effect of budesonide, and improves the treatment efficiency.

The invention belongs to the technical field of biological medicines, discloses application of bifidobacterium breve BB990 in the field of metabolism, and particularly discloses bifidobacterium breve BB990 which is preserved in Guangdong Microorganism Culture Collection Center, the preservation number is GDMCC No.63259, and the preservation date is March 10, 2023. The invention further discloses a preparation method of the bifidobacterium breve BB990. The bifidobacterium breve BB990 provided by the invention has the effects of regulating gastrointestinal disorder, enhancing intestinal immune function, inhibiting harmful bacteria, improving intestinal flora structure, maintaining intestinal steady state, regulating immunity, improving immune curative effect, regulating body glycometabolism and liver metabolism and the like. Experiments prove that the bifidobacterium breve BB990 can effectively prevent and treat metabolic diseases, such as non-alcoholic fatty liver, type II diabetes and the like, the treatment effect of the bifidobacterium breve BB990 is remarkably superior to that of other bifidobacterium breve (such as bifidobacterium breve 206), and the bifidobacterium breve BB990 has a good prospect.

The invention provides an Akkermann Amuc1100 protein mutant as well as a preparation method and application thereof, wild Akkermann Amuc1100 protein is subjected to mutation modification through a molecular biological technical means, and the bioactivity of the Amuc1100 protein mutant subjected to molecular modification is obviously improved compared with that of the wild protein; compared with a wild type Amuc1100 protein, the wild type Amuc1100 protein has a better effect when being applied to the aspects of regulating intestinal immunity, relieving acute pancreatitis, promoting browning of white adipose cells, inhibiting obesity, relieving ulcerative colitis and the like, and a very good application prospect is provided for developing bioactive medicines with higher efficiency.

The invention relates to the technical field of cell biology, in particular to a construction method and application of intestinal organs for children with Crohn's disease, the intestinal organs for children with Crohn's disease are successfully constructed by utilizing intestinal tissues from children with Crohn's disease, and the intestinal organs have various characteristic cells of the intestinal tissues; besides, immune cells are extracted from peripheral blood of a child patient homologous with intestinal tissues, a co-culture system is established with the organ-like model, the intestinal immune microenvironment of the child patient suffering from the Crohn's disease can be reproduced, and the system provides a new model for related mechanism exploration and drug screening of the child Crohn's disease.

The invention relates to the technical field of microorganisms, in particular to compound probiotics capable of degrading indole and preventing or relieving nephropathy and application of the compound probiotics. The compound probiotics comprise bifidobacterium animalis subsp. Lactis YYS-J9 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.28185 and bifidobacterium longum subsp. Longum YYS-BSM8 with the preservation number of CGMCC NO.28611. The invention further discloses a preparation method of the compound probiotics. Compared with the use of a single YYS-J9 strain or YYS-BSM8 strain, the two strains have synergistic effects after being compounded, the viable count is remarkably increased after mixed culture, the inhibition effect on pathogenic enterobacteria and the adhesion to intestinal cells are remarkably enhanced, the degradation capacity on indole and oxalic acid is higher, the proliferation and phagocytic activity of macrophages is remarkably improved, and the application range of the YYS-J9 strain and the YYS-BSM8 strain is widened. And when being applied to food, health-care products or medicines, the exopolysaccharide can be used for effectively preventing or improving nephropathy and kidney stone, regulating intestinal health and improving intestinal immunity.

The invention provides a patient consultation intelligent reply method and system based on an RAG knowledge base, and is applied to the technical field of data processing. The method comprises the following steps: preprocessing multi-modal data of a patient to generate text semantic features, voice features, intestinal barrier function features, intestinal immune function features, intestinal nerve function features and image features; performing feature extraction processing on the historical dialogue data of the patient to generate past consultation features and medication feedback features; processing the previous consultation features and the medication feedback features to generate patient portrait information; preprocessing the medical knowledge base data, extracting preset semantic features and storing the preset semantic features to a mixed index structure; the intestinal barrier function features, the intestinal immune function features and the intestinal nerve function features are processed, and intestinal influence factors are generated; and processing the text semantic features, the image features, the patient portrait information and the intestinal tract influence factors based on the dynamically updated medical knowledge base data and an RAG technology to generate target reply information.

The invention discloses a preparation method of a lactoferrin-polyphenol nano compound with an intestinal immune function. The method comprises the following steps: firstly, moderately hydrolyzing lactoferrin under the action of compound protease, and optimizing a protein molecular structure; then, compounding with water-soluble polyphenol (such as chlorogenic acid or epicatechin) by utilizing a metal ion chelating technology, and inducing lactoferrin to be bridged and aggregated by Ca to form a compound; and then, carrying out ultrasonic homogenization treatment, and further controlling the particle size to enable the particle size to be distributed in a range of 100-300 nm. The obtained compound is subjected to nanofiltration concentration and electrostatic spray drying to obtain a shell-core nano compound with a stable structure, and nitrogen-filled packaging is adopted to prevent oxidation. The lactoferrin-polyphenol nano-composite prepared by the invention not only has good stability and intestinal immunity enhancement effect, but also has remarkable antibacterial activity, and can be widely applied to functional foods and nutritional health-care products.

The invention provides a feed additive capable of improving immunity of pet cats as well as a preparation method and application of the feed additive, and belongs to the technical field of animal feed additives. The feed additive comprises probiotics and a traditional Chinese medicine extract, the probiotics comprise bifidobacterium animalis, combined lactobacillus salivarius and saccharomyces cerevisiae boulardii, and the traditional Chinese medicine extract comprises astragalus polysaccharide, curcumin and resveratrol. The probiotics directly enhance intestinal immunity through colonization resistance and metabolites, the traditional Chinese medicine components form internal and external dual defense through system immune activation, immunity-flora dual regulation is achieved, the probiotics and the traditional Chinese medicine extract form a symbiotic system of increasing the drug effect through bacteria and protecting the bacteria through drugs, and 1 + 1gt is achieved; and 2, the immunity improving effect is achieved.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

The invention provides a feed for relieving weaning stress of lambs and a preparation method of the feed, and belongs to the field of animal feeds. The feed disclosed by the invention is prepared from the following components in parts by weight: 35-40 parts of puffed corn, 15-20 parts of enzymolysis soybean meal, 5-8 parts of fish meal, 5-8 parts of alfalfa meal, 0.3-3 parts of an organic trace element compound, 0.1-2 parts of a vitamin compound, 3-5 parts of whey powder, 0.5-1 part of butyrin, 0.3-0.5 part of glutamine and 0.2-0.3 part of a radix astragali-spina date seed compound extract. 0.2 to 0.3 part of xylooligosaccharide-xylanase compound, 0.05 to 0.1 part of pancreatin active peptide and 0.1 to 2 parts of complex microbial inoculant. The weaning stress of the lambs is reduced through a targeted intestinal tract-immunity-nerve regulation ternary synergistic formula, the digestive system development of the weaned lambs can be obviously improved, the activity of digestive enzymes can be improved, and therefore the weight gain of the lambs is obviously improved, and the lambs stably pass through the weaning period.

Provided are a substance and method for improving intestinal health and homeostasis, and the nutrition and development of a subject. The substance is an odd carbon fatty acid. Specifically, provided is the use of an odd carbon fatty acid in the preparation of a preparation or drug for increasing the protein expression abundance of related tight junction proteins of a subject, reducing the content of a corticotropin-releasing hormone in the intestinal tissue of a subject, improving or enhancing the intestinal mechanical barrier of a subject, maintaining the intestinal immune homeostasis of a subject, promoting the intestinal development of a subject, maintaining the energy homeostasis in intestinal cells of a subject, improving intestinal energy metabolism, promoting the development and metabolism of muscle tissue in a subject, promoting the development and metabolism of adipose tissue in a subject, reducing allergic reactions of the body and the intestinal tract of a subject, reducing the insulin resistance in a pregnant subject, controlling the blood glucose level in a pregnant subject, preventing gestational diabetes mellitus, preventing or improving intrahepatic cholestasis in a pregnant subject, preventing or treating diseases caused by Helicobacter pylori bacteria in a subject, etc.

The invention discloses a plant lactobacillus PC037 capable of improving intestinal immunity and a fermented product. The plant lactobacillus PC037 is preserved in the China General Microbiological Culture Collection Center on December 13, 2021, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 24096. The plant lactobacillus PC037 can improve the repair speed of the intestinal mucosa and promote the enhancement of the immunity of the intestinal tract. The plant lactobacillus fermented product capable of improving the immunity of the intestinal tract, provided by the invention, can be used for adjusting the exhaust frequency, promoting the enhancement of the immunity of the intestinal tract and reducing the occurrence frequency of diarrhea caused by pathogenic bacteria infection of travelers in a travel process.

The invention discloses an enzymolysis preparation method of high-immunocompetence bovine coloctrum peptide, active peptide and application thereof, and belongs to the field of biotechnology and functional foods.The enzymolysis preparation method is characterized by comprising the steps that bovine coloctrum is degreased, trehalose is added into obtained supernate, first stirring is conducted, and first mixed liquid is obtained; wherein the supernate comprises whey protein and Ig; adding medium protease into the first mixed solution for hydrolysis to obtain enzymatic hydrolysate; wherein the enzymatic hydrolysate comprises a mixture of bovine colostrum hydrolyzed peptides, the Ig activity retention rate is larger than or equal to 90% through the targeted protection and directional enzymolysis technology, the beta-lactoglobulin peptide with the synergistic immune enhancement effect is obtained, the Ig and small molecule peptides in the product have the synergistic effect, the body immune regulation capacity and the intestinal immune function are remarkably improved, the immunity of the human body is improved, and the immunity of the human body is improved. The method can be widely applied to the fields of infant formulas, postoperative nutrition, functional drinks and the like.

The invention relates to an establishment method and application of in-vitro intestinal inflammation models of mice of different ages, and relates to the technical field of biology. According to the invention, young, adult, middle-aged and old wild type (WT) and TLR4 gene deletion type (TLR4 <- / ->) mice are selected, colon tissues are cultured in vitro, inflammatory response is induced by using interleukin-1beta (IL-1beta) stimulation, an age-related intestinal inflammation model is constructed, the inflammation phenotype of the mouse model and the activity of a TLR4 / MyD88 / NF-kappa B signaling pathway are determined by comparing WT and TLR4 <- / ->, and the age-related intestinal inflammation model is constructed. The core function of the TLR4 in regulating age-dependent intestinal barrier dysfunction is defined, a regulatory network of the TLR4 in intestinal immune aging and flora-host interaction is disclosed, and an age personalized medical strategy is promoted. According to the invention, an efficient and customizable multi-age in-vitro intestinal inflammation model is constructed, and an innovative platform is provided for mechanism analysis and intervention drug screening of TLR4 targeted therapy and age-related intestinal diseases.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention relates to a composition for regulating intestinal microenvironment and relaxing bowel and a preparation method thereof, and belongs to the technical field of biology. The composition comprises the following components in specific parts: artichoke-siraitia grosvenorii-fructus cannabis composite extract peptide, bacillus coagulans-lactobacillus reuteri calcium alginate microspheres, escherichia coli bacteriocin, salmonella bacteriocin, a liquorice extract, a semen cassiae extract, a herba cistanche extract, a dendrobium officinale extract and the like. Wherein the artichoke-siraitia grosvenorii-fructus cannabis compound extract peptide, bacillus coagulans-lactobacillus reuteri calcium alginate microspheres, the liquorice extract, the semen cassiae extract, the herba cistanche extract and the dendrobium officinale extract are obtained by a special preparation method. Through cooperation of the components, the intestinal microenvironment can be adjusted from multiple angles, and the comprehensive effects of improving the intestinal flora structure, inhibiting harmful bacteria, promoting the growth of beneficial bacteria, lubricating the intestinal tract, relieving intestinal inflammation, enhancing the intestinal mucosal barrier function, improving the intestinal immunity and the like are achieved.

The invention belongs to the field of food nutrition and functional factors, and particularly relates to a method for preparing ovotransferrin hydrolysate with high immunocompetence based on an adult gastrointestinal simulated digestion system. According to the method, ovotransferrin is treated through the stomach and intestine of a simulated adult, OVT hydrolysate is prepared, and the OVT hydrolysate contains HTEGSTT, TTSY and / or RTAGWVIPMG. The invention finds that the immunomodulatory activity of OVT is enhanced after gastrointestinal digestion and hydrolysis, the OVT is more easily hydrolyzed in simulated adult digestive juice than in infant digestive juice, and a contrast experiment shows that the nutrition intervention of OVT adult and infant simulated digestion products can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid; the swelling injury caused by inflammation to small intestine mucous membrane tissues is effectively relieved, so that the intestinal barrier immune function is obviously improved, and the OIA shows higher intestinal immune activity than the OIB.

The invention provides a composition for promoting differentiation of intestinal immune cells as well as a preparation method and application of the composition. According to the composition, 1, 3-dioleic acid-2-palmitic acid triglyceride and breast milk oligosaccharide are used in a combined manner, so that monocytes can be effectively promoted to differentiate into intestinal macrophages, and inflammation is reduced to a certain extent. After the monocytes are differentiated into the intestinal macrophages, the intestinal immune homeostasis and intestinal microbiota inflammatory response can be regulated, so that the mucosal immune homeostasis and intestinal epithelial cell barrier function are maintained. Therefore, the composition provided by the invention can improve the intestinal immune barrier by promoting the monocytes to differentiate into the intestinal macrophages, and provides a new thought for improving the early intestinal barrier of infants through nutritional supplement.

The invention provides a preparation method and application of polygonatum cyrtonema acidic polysaccharide, and belongs to the technical field of polygonatum cyrtonema polysaccharide extraction. According to the invention, a separation and purification method combining anion exchange chromatography with gel chromatography is adopted, and finally the polygonatum cyrtonema acidic homogeneous polysaccharide (PCPA) with relatively high purity is obtained. An in-vivo pharmacodynamic experiment shows that the prepared PCPA can remarkably relieve intestinal mucosa injury and has relatively high intestinal immune regulation activity. The preparation method provided by the invention is stable in process, and the prepared polygonatum cyrtonema acidic polysaccharide is high in purity, is a homogeneous polysaccharide and has the effect of regulating intestinal immunity.

The present application relates to a kind of composition for adjusting intestinal flora and its preparation method and application, and the raw materials of the composition include: fried atractylodes, dried tangerine or orange peel, fried liquorice, magnolia officinalis (ginger preparation), polyporus umbellatus, poria cocos (skin removal), fried atractylodes, alisma orientalis, cassia bark, dried ginger, dark plum.The present application produces the effect of anti-inflammatory, antioxidant, regulating intestinal flora, improving intestinal immunity and intestinal barrier protection effect etc. through the synergistic effect between raw materials.

The invention relates to the technical field of microorganisms, in particular to compound probiotics for relaxing bowel and improving intestinal immunity and application thereof. The compound probiotics comprise a plant lactobacillus BXM2 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.16436 and a bifidobacterium longum subsp. Longum YYS-BSM8 strain with the preservation number of CGMCC NO.28611, and the ratio of the viable count of the plant lactobacillus BXM2 to the viable count of the bifidobacterium longum subsp. Longum YYS-BSM8 is (1-10): (1-10). After the two bacteria are compounded and combined, the gastrointestinal motility efficiency of zebra fish is accelerated, constipation is relieved, enteropathogenic bacteria are inhibited, the adhesive force of intestinal cells is improved, exopolysaccharides are produced at high yield, proliferation and phagocytic activity of macrophages are promoted, oxidative damage is remarkably relieved, and synergistic interaction is achieved in the aspects of improving the intestinal motility efficiency, relieving constipation and improving body immunity; the important application value is realized.

The invention relates to livestock feeding probiotics, in particular to bacillus subtilis and application, the bacillus subtilis is bacillus subtilis BW and is preserved in the China General Microbiological Culture Collection Center on April 10, 2025, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) 0.34165. The bacillus subtilis BW disclosed by the invention can be colonized in animal bodies, so that the diarrhea rate of piglets is remarkably reduced, the immunity of intestinal tracts is improved, and the health of the intestinal tracts is improved; after the bacillus subtilis BW is used for feeding ruminants, the feed conversion efficiency can be remarkably improved, the growth performance can be improved, and the bacillus subtilis BW has a good application prospect in livestock and poultry breeding.

The invention relates to application of microalgae polysaccharide in improving intestinal immunity of dairy cows, belongs to the technical field of feeds, and the microalgae polysaccharide prepared by the invention is used for improving the secretion volume of intestinal mucosa to lgA, lgG and lgM. Kelp buds, dunaliella salina and haematococcus pluvialis are compounded and mixed according to a specific proportion, trypsin and nattokinase are adopted for pre-enzymolysis within a certain period of time, then hemicellulase and pectinase are adopted for enzymolysis within a certain period of time, and the enzymolysis effect is good. The obtained microalgae polysaccharide product has an excellent effect of promoting the intestinal tract of the dairy cow to secrete immune globulin, the content of IgA, IgG and IgM in the intestinal tract of the dairy cow can be greatly increased, the immunity of the intestinal tract of the dairy cow is further improved, and the microalgae polysaccharide product can be used as a substitute component for efficiently improving the immune function of the intestinal tract of the dairy cow.

The invention discloses a clostridium praeparatum vesicle targeting inflammatory bowel disease tissues as well as preparation and application of the clostridium praeparatum vesicle. The vesicle is secreted by clostridium praeparatum ATCC27766, and the average particle size of the vesicle is 100-250 nm. Experimental data show that the vesicles have intestinal disease site targeting, can effectively remodel the intestinal immune microenvironment of ulcerative colitis patients, and inhibit the inflammation process through chemotactic factor secretion of macrophages. The clostridium praeparatum vesicle and the preparation can effectively overcome the defect that clostridium praeparatum and butyrate secreted by the clostridium praeparatum are prone to failure, the treatment means of ulcerative colitis are enriched, and the application prospect of anaerobic probiotics is widened.

The invention relates to the technical field of chemical toxicology, in particular to an evaluation method for comprehensively analyzing the immune induction effect of a plasticizer by using caenorhabditis elegans, according to the evaluation method, a mother worm breeding mode is changed to replace a lysis solution to synchronize nematodes, experimental interference is reduced, the result accuracy is improved, monitoring indexes are newly increased, intestinal injuries and in-vivo bacterial indexes are increased, and the evaluation method is suitable for comprehensive analysis of the immune induction effect of the plasticizer. The evaluation is more accurate and reliable by combining oxidative stress indexes and performing two-dimensional comprehensive evaluation from an intestinal immune defense line and oxidative stress, and by drawing a multi-index monitoring shape graph, adopting a comprehensive biomarker response index analysis method and associating immune-related representative indexes, the evaluation accuracy is improved. According to the method, the change of each index and the immunity intensity on the caenorhabditis elegans under different exposure conditions are intuitively presented, the immune defense effect of the caenorhabditis elegans under the stress of the plasticizer can be quickly and effectively evaluated without transcriptome and metabonomics, and efficient evaluation is realized.

The invention discloses a method for preparing a high-fiber gel minced fillet product based on low-molecular-weight tremella polysaccharide. The method comprises the following steps: modifying the low-molecular-weight tremella polysaccharide; dissolving the modified low-molecular-weight tremella polysaccharide in water to obtain tremella homogenate; and preparing the minced fillet: mixing fish meat with the modified low-molecular-weight tremella polysaccharide, adding table salt and auxiliary materials, and heating and curing to obtain the high-fiber gel minced fillet product. According to the tremella polysaccharide surimi composite product prepared by the preparation method disclosed by the invention, the modified tremella polysaccharide slurry is added in the surimi processing process, so that the gel performance of surimi can be well improved, the surimi has high fiber property, the smooth and elastic mouth feel of the surimi product can be enhanced, and the taste bud experience is richer. Meanwhile, as the tremella polysaccharide has rich biological activity, such as antioxidation, intestinal immunity regulation, blood sugar and blood fat reduction and the like, the tremella polysaccharide can be added into the surimi product as one of the raw materials, and additional functional characteristics can be endowed to the surimi product.

The application belongs to the technical field of biological nanomaterials, and particularly relates to an immune activation nanosheet as well as a preparation method and application thereof. The immune activation nanosheet comprises a two-dimensional montmorillonite nanosheet with a sheet layer structure, and manganese ions and tumor antigens loaded in the interlayer of the two-dimensional montmorillonite nanosheet. The mass ratio of the manganese ions to the montmorillonite is 1:5-1:20, and the mass ratio of the tumor antigens to the montmorillonite is 1:1-1:10. The immune activation nanosheet has the characteristics of good oral stability, high mucosal targeting, strong immune efficacy, etc. The stability of the immune activation nanosheet in the gastrointestinal tract is good, the immune activation nanosheet can be effectively enriched in the small intestine, a vaccine inoculation pool is formed, the intestinal immune tolerance can be overcome, the immune effect is enhanced, and the immune activation nanosheet has a clinical application prospect.

The invention relates to the technical field of medicines, and provides a PMC nano-enzyme and a preparation method thereof. The PMC nano-enzyme comprises: a core layer, wherein the core layer comprises oxygen atoms, phosphate ions and a polyoxometallate nano-cluster of a first transition metal element; at least part of the coating layer is fixed on the outer surface of the core layer, and the coating layer is formed through coordination between second transition metal and oxygen atoms on the core layer; wherein the first transition metal element comprises Mo; the second transition metal element includes Mn, and the coating layer includes a CO donor for controlling CO gas release. According to the PMC nano-enzyme disclosed by the invention, the polyoxometallate nano-cluster can be combined with a CO gas therapy to synergistically treat IBD, on the basis of endowing the nano-cluster with anti-inflammatory and intestinal epithelial barrier repair promoting functions, the catalytic activity is enhanced, and CO is released in an active oxygen overexpression environment, so that oxidative stress is relieved, inflammatory response is inhibited, intestinal immune balance is recovered, and the effect of treating IBD is achieved. The repair of mucosal injury is promoted.