89 results about "Lymphatic tissues" patented technology

The invention discloses construction and application of an oral gel drug delivery system based on a yeast microcapsule, and belongs to the field of new auxiliary materials and new dosage forms of pharmaceutical preparations, the drug delivery system is composed of budesonide nanoparticles, the yeast microcapsule and gel, budesonide nanoparticles are prepared from budesonide and a polylactic acid-glycolic acid copolymer through a water-in-oil emulsification evaporation technology, yeast cell wall microcapsules are formed after content of yeast powder is removed, and the budesonide-loaded yeast microcapsules are prepared from the yeast microcapsules and the budesonide nanoparticles through stirring, ultrasonic treatment and freeze drying methods. The yeast cell wall microcapsule without contents is used as a carrier, so that the medicine is protected from being degraded by gastric acid, and the intestinal immune response is regulated by targeting intestinal related lymphatic tissues (GALT). The gel prepared by combining pectin can prolong the residence time of the drug in the intestinal tract, realizes continuous release, reduces the whole-body side effect of budesonide, and improves the treatment efficiency.

The invention relates to a method for detecting a B cell subset in a lymphatic tissue, a kit comprising a quantum dot-antibody CD19 conjugate, a quantum dot-antibody CD138 conjugate and a quantum dot-antibody CD1d conjugate is used for dyeing a lymphatic tissue section, and different antibodies are marked by quantum dots with different colors, so that the B cell subset in the lymphatic tissue section can be detected, and the B cell subset in the lymphatic tissue section can be detected. Content and positions of plasma cells and regulating B cells are confirmed, so that biological behaviors of the plasma cells and regulating B cells can be determined conveniently. According to the method, the existing multi-color immunofluorescence technology is improved, a secondary antibody dyeing link is omitted by using a quantum dot carrying antibody method, a direct method is adopted for marking, the advantages of quantum dots are exerted, the experiment time is saved, and the efficiency is improved.

A spore of a bacillus subtilis B.subtilis WB600 strain is used as an antigen delivery carrier, spore coat protein C (CotC) is used as anchoring protein, a key protein ATPase gene and a CotC gene of micropterus salmoides iridovirus are fused through a gene fusion technology, a recombinant spore surface display system is constructed, the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. After being orally applied to fishes, the vaccine can tolerate the gastrointestinal tract environment and is accurately delivered to intestinal related lymphatic tissues to stimulate fish immunity, and finally prevention and control of the iridovirus of the micropterus salmoides are achieved.

A kit for detecting expression of AIRE and Treg in lymphatic tissue infected by EB virus comprises colloidal quantum well antibody conjugates, including a colloidal quantum well and protein AIRE and GITR conjugates and a colloidal quantum well and antibody CD25 and Foxp3 conjugates. The detection method comprises the following steps: (1) carrying out slicing treatment on a lymphatic tissue, and carrying out antigen repair by using an antigen repair liquid; (2) respectively adding colloidal quantum well proteins or antibodies coupled with different targets to dye the sections; (3) carrying out nucleus staining; and (4) collecting multi-channel fluorescence image information of the tissue slice, and judging expression levels and mutual relations of AIRE, Treg cells and dendritic cells in immune tolerance in combination with spatial positioning and intensity distribution of fluorescence signals of different channels. According to the invention, qualitative or quantitative multi-parameter and high-precision detection of the immune tolerance related cells in the lymph tissue is realized.

The invention discloses an intelligent classification and recognition method for ear-nose-throat lesion images, and relates to the technical field of image recognition, and the method comprises the steps: synchronously collecting a nasopharynx white light image and an i-Scan image, screening an image meeting a quality standard, carrying out the correlation of pathology and grading labeling, and constructing a data set containing a multi-center case; the white light image and the i-Scan image are subjected to denoising processing, key mark points are extracted, image registration is completed, a mucous membrane surface area, a submucous membrane blood vessel area and a lymphatic tissue enrichment area are divided through semantic segmentation, and feature enhancement is carried out on different areas; extracting morphological features and textural features from the enhanced image, calculating feature importance weights and fusing the feature importance weights into low-dimensional feature vectors; the benign and malignant dichotomy model and the pathological type multi-classification model are respectively constructed, and classification results are output in combination with a doctor diagnosis rule base and verification indexes, so that the accuracy and clinical practicability of ear-nose-throat lesion diagnosis are remarkably improved.

The invention provides pathological tissue section dyeing equipment and method. The method comprises the following steps: dividing a to-be-dyed lymphatic tissue section into a plurality of dyeing partitions according to the structural characteristics of lymphocyte tissues in the to-be-dyed lymphatic tissue section; dyeing agents with different concentrations are applied to different dyeing partitions according to the reference light transmittance of the lymphocyte tissue to obtain color gradient vectors of the lymphocyte tissue slices, light absorption characteristics of the dyeing partitions are measured through a photometer, and dyeing characteristics are determined based on the light absorption characteristics and the color gradient vectors; obtaining a dyeing image after dyeing the lymph tissue section, extracting deep features of the dyeing partitions from the dyeing image, and determining a dyeing loss value when dyeing the dyeing partitions based on the deep features and the dyeing features; and based on the dyeing loss value, carrying out self-adaptive adjustment on the dyeing agent concentration of each dyeing zone. By adopting the scheme provided by the invention, optimal adjustment on the concentration of the coloring agent in the sample dyeing process can be realized based on the characteristic difference of the cell tissues at different parts.

The invention relates to a kit and a method for simultaneously detecting IL-6, IL-21 and T cell CD4 surface molecules in lymphatic tissue, and belongs to the field of biomedicine. The kit comprises a quantum dot marked with an IL-6 antibody, a quantum dot marked with an IL-21 antibody and a quantum dot marked with a CD4 antibody. The antibodies marked by the quantum dots are specifically combined with antigens in lymphatic tissues respectively; the CD4T cells have the capacity of secreting IL-6 and IL-21, and the mutual relation between the CD4T cells and IL-6 and IL-21 reflects the tissue immunocompetence. The invention provides a kit for simultaneously detecting IL-6, IL-21 and T cell surface molecules in lymphatic tissues, which positions the spatial relationship between two cell factors and T cells, calculates and analyzes corresponding fluorescence intensity and evaluates immune conditions. The kit has the advantages of high fluorescence intensity, accurate detection result, wide spectrum selection, wide applicability, stable fluorescence structure, long preservation time, high sensitivity, good specificity and the like.

The invention relates to a lymphatic drainage device. The lymphatic drainage device comprises an anti-blocking support, a collecting cavity is formed in the anti-blocking support, the anti-blocking support is used for supporting biological tissue on the periphery of a to-be-drained position so as to maintain the shape of the collecting cavity, and the collecting cavity is used for collecting lymph generated by lymphatic tissue on the periphery of the to-be-drained position; the two ends of the drainage tube are an inlet end and an outlet end, the inlet end is used for guiding the lymph into the drainage tube, the outlet end is used for guiding the lymph out of the drainage tube, and the inlet end extends into the collecting cavity; the implantable pump is connected to the drainage tube and is used for pumping the lymph in the collecting cavity from the inlet end of the drainage tube and pumping the lymph out from the outlet end of the drainage tube. By designing the lymph drainage device with the function of automatically adjusting negative pressure suction, continuous drainage of neck lymph is achieved.

The invention discloses a ganoderma lucidum peptide for enhancing immunity as well as a preparation method and application thereof, and belongs to the technical field of functional peptide preparation. The ganoderma lucidum peptide provided by the invention contains various active ingredients / nutritional ingredients, is high in utilization rate of ganoderma lucidum, can improve the health level of intestinal tracts, promotes absorption and utilization of nutrient substances by the intestinal tracts, and provides a fundamental material guarantee for improving the immunity of the organism; immune cells are activated, and related lymphatic tissues of intestinal tracts are stimulated, so that the effects of regulating cellular immunity and humoral immunity levels are achieved; the preparation method provided by the invention does not use high-temperature, high-pressure and toxic and harmful reagents, has mild conditions, and is simple, easy to operate and environment-friendly; no toxic and harmful substances are generated, and generated filter residues can be directly used as animal feed or plant fertilizer; the prepared ganoderma lucidum peptide is small in molecule, high in activity, rich in active substance variety, high in yield and easy to absorb and utilize by organisms.

The invention discloses a culture method of in-vitro uniform monolayer cells of prawns, and belongs to the technical field of biology. The method comprises the following steps: (1) obtaining lymphatic tissues of a last-stage prawn at the early stage of shelling, completely stripping an outer membrane to obtain internal tissues, and cutting the internal tissues into small tissue blocks; (2) cleaning the small tissue blocks, inoculating the small tissue blocks to a culture plate coated with a gelatin solution, adding a complete culture solution into an inoculation system at intervals within 12 hours after inoculation for culture, replacing the complete culture solution 24-48 hours after inoculation, and inducing tissue cells to continuously migrate to form an in-vitro cell layer; (3) blowing and beating the cell culture to obtain a cell suspension, and centrifuging to obtain a cell monolayer; and (4) suspending the cell monolayer by adopting a complete culture solution, then inoculating the cell monolayer into a culture plate coated with a gelatin solution, and culturing to obtain the in-vitro uniform monolayer cells of the prawns. According to the method, the prawn monolayer cells which are long in in-vitro survival time, uniform and stable and are cultured in vitro can be obtained, and an important tool is provided for related virology research.

The present disclosure provides compositions and methods for the treatment and prevention of macrophage activation syndrome (MAS) or hemophagic lymphocyte hyperplasia (HLH). The methods involve administering to a patient in need thereof an antibody or fragment thereof that is specific for human GM-CSF protein.

The present invention relates to a compound of formula (I), which is characterized as PROTAC for MALT1. The PROTACs described herein can be used to treat diseases or conditions associated with MALT1, such as lymphoma. In particular, the present invention relates to compounds and pharmaceutical compositions capable of degrading MALT1, methods of treating diseases or conditions associated with MALT1, and methods of synthesizing these compounds. I) # imgabs0 #

The present disclosure provides, inter alia, anti-peripheral lymph node addressin antibodies and antigen binding fragments thereof. The present disclosure also provides compositions comprising drug-containing polymeric particles that mimic lymphocyte migration in vivo and can specifically deliver immunosuppressive or immunoregulatory drugs to lymphoid tissues and sites of chronic inflammation where T-cell activation and T-cell mediated injury are occurring; such compositions comprise the antibodies or antigen-binding fragments thereof described in the disclosure. The present disclosure also comprises antibody-drug conjugates and compositions comprising the antibody-drug conjugates. Methods of preparing and using these antibodies, antigen-binding fragments thereof, and compositions thereof are also provided.

The present disclosure relates to the technical field of chemoimmunology, and in particular to an anti-receptor expressed on lymphoid tissues (RELT) recombinant monoclonal antibody and a preparation method and use thereof. The anti-RELT recombinant monoclonal antibody includes a heavy chain variable region and a light chain variable region, where the amino acid sequence of the heavy chain variable region is set forth in SEQ ID NO: 4; the amino acid sequence of the light chain variable region is set forth in SEQ ID NO: 5; and the anti-RELT recombinant monoclonal antibody has a biological activity of neutralizing a function of RELT. In addition, because the anti-RELT recombinant monoclonal antibody has the biological activity of neutralizing the function of RELT, the anti-RELT recombinant monoclonal antibody can specifically recognize and detect the expression of RELT in different tissues and cells.

One purpose of the present disclosure is to provide a technology for testing the presence or absence of a TLT in a subject without biopsy. Disclosed is a method for testing the presence or absence of a tertiary lymphoid tissue in a subject, the method including the step of measuring a soluble CD30 concentration in a blood sample collected from the subject.

Viruses, bacteria, and parasites frequently cause infections in the gastrointestinal (GI) tract, but traditional vaccination strategies typically elicit little or no mucosal antibody responses. Disclosed herein is a vaccine comprising a nanoemulsion and an immunogen non-covalently conjugated to the surface of the nanoemulsion by an amphiphilic linker, wherein the vaccine is suitable for inducing a mucosal antibody response in the gastrointestinal tract. Also disclosed are methods of using the vaccine to immunize a subject by intraperitoneal administration of an effective amount of the vaccine, alone or with an adjuvant.

The invention relates to a kit for simultaneously detecting angiogenesis and plasma cell infiltration in lymphatic tissue and a preparation method and application of the kit, the kit comprises quantum well-antibody conjugates CQWs-525-VEGF, CQWs-525-ACTA2 and CQWs-585-CD138 and CQWs-625-Mum1, and the kit can be used for staining a lymphatic tissue section; by marking expression conditions of VEGF, ACTA2, CD138 and Mum1, the angiogenesis and plasma cell infiltration degree in the lymphatic tissue is evaluated. The quantum sheet has the characteristics of wide absorption spectrum, narrow light-emitting spectrum, high brightness and difficulty in quenching, and is used for marking blood vessels and plasma cells in tissues after being coupled with an antibody, so that quantitative analysis can be realized and spatial position distribution can be observed at the same time, the immune microenvironment of the tissues can be more accurately and effectively detected, and the immune state and immune function of the organism can be further evaluated.

The invention relates to the technical field of food processing, in particular to continuous raw material cleaning equipment for food processing. The problem that an existing device cannot rapidly and continuously remove the lymphatic tissue in the duck neck is solved. Comprising a workbench, and an isolation shell is fixedly connected to the workbench; the two sliding blocks are connected to the workbench in a limiting and sliding mode, and the sliding blocks are connected with two sliding rods in a sliding mode; the fixing frame is fixedly connected into the isolation shell, a sliding supporting rod is connected to the fixing frame in a sliding mode, a blade is installed at the lower end of the sliding supporting rod, a connecting block is arranged on the fixing frame, and the connecting block is connected with a sliding plate in a sliding mode. The duck necks are fixed through the sliding rods, the operation that the blades on the sliding supporting rods cut open the belly of the duck necks and the sliding plates scrape the lymphatic tissues in the duck necks is combined, the purpose of continuously removing the lymphatic tissues of the multiple duck necks is achieved, the duck neck cleaning efficiency is improved, and the production efficiency of marinated duck necks is improved.

A three-dimensional (3D) lymphoid tissue model is provided, the model including a cellularized stromal compartment and a plurality of cellularized compartments including lymphocytes disposed within the stromal compartment; and a controlled fluid perfusion system configured to perfuse the model with a perfusion fluid. Methods of fabricating a 3D lymphoid tissue model and producing antibodies with the 3D lymphoid tissue model are also provided.

The present invention relates to the technical field of lymphedema auxiliary treatment, and specifically to an adjustable lymphedema auxiliary treatment device for breast cancer surgery, comprising a base, one end of the base is fixedly connected to a motor, the output end of the motor is connected to a reciprocating screw, a slider is arranged on the outside of the reciprocating screw, the upper end of the slider is connected to a support plate, an air bag is arranged inside the support plate, a connecting pipe is connected to the outside of the air bag, and the end of the connecting pipe away from the air bag is connected to an air pressure chamber. When in use, the present invention squeezes the push rod to contract toward the inside of the air pressure chamber by the slider, so that the air pressure enters the inside of the air bag through the connecting pipe, so that the air bag expands and squeezes the patient's arm, thereby forming a uniform and orderly squeezing of the limb from the distal end to the proximal end, simulating professional massage techniques, promoting the flow of blood and lymphatic tissue and improving microcirculation, accelerating the reflux of limb tissue fluid, effectively reducing edema, and preventing muscle atrophy and fibrosis.

The rapid lymphatic vein communication device comprises an elastic tube body, notches which are symmetrical left and right are formed in the front end of the tube body, the notches are in the shape with the large front portion and the small rear portion, the tube thickness of the notch areas is sequentially decreased from back to front, the section of the tube body is in an oval shape, and the section of the tube body is in an oval shape. The upper end and the lower end of the tube body are provided with sheet-shaped protrusions on the right side of the notch, and lymphatic vessel generation promoting factors are embedded or coated on the tube wall of the tube body. According to the invention, lymphatic tissues and veins can be communicated efficiently and quickly; blood reflux and thrombosis are avoided; sewing operation is not needed or reduced; blood vessel blocking is not needed; specific growth factors contained in the kit can promote the generation of normal lymph lumen, form autologous biological lumen in the later period, and maintain long-term smoothness.

The method (400) includes receiving an input tissue structure image (110), generating one or more lymphocyte density maps (125) inside the input tissue structure image by processing the input tissue structure image using a cell classification model (550), and identifying one or more TLS regions (135) inside the input tissue structure image by performing morphological image processing (130) on the one or more lymphocyte density maps. Each TLS region is represented by each cluster of lymphocyte cells. For each corresponding TLS region of the one or more TLS regions identified in the input tissue structure image, the method includes extracting each set of TLS features (140) from each cluster of lymphocyte cells, and classifying the corresponding TLS region as one of a first TLS maturation state, a second TLS maturation state, and a third TLS maturation state by processing each set of TLS features using a TLS classification model (350).

The present invention relates to a composition for the cryopreservation of primary dendritic cells, and a method for cryopreservation and cell culture using same, and more specifically to a composition for the cryopreservation of primary dendritic cells, and a method for cryopreservation and cell culture using same, the composition comprising serum and DMSO, thereby enabling a high viability after thawing of primary dendritic cells, and being capable of inhibiting the spontaneous activation of the primary dendritic cells so as to enable same to be cultured in vitro, and being usable for immunological diagnosis and therapeutic agent development for various diseases.

The present disclosure provides, inter alia, compositions comprising drug-containing polymeric particles that mimic lymphocyte migration in vivo and can specifically deliver immunosuppressive or immunoregulatory drugs to lymphoid tissues and sites of chronic inflammation where T-cell activation and T-cell mediated injury are occurring. Methods of preparing and using these drug-containing polymeric particles are also provided.

The invention provides a composite hydrogel for lymphatic tissue repair, which is formed by photo-crosslinking a gelatin-dopamine hydrogel capable of being subjected to methacrylic acid esterification, a lymph node acellular matrix and a photo-crosslinking initiator. The invention also provides a preparation method of the composite hydrogel, a re-cellularized hydrogel and application of the composite hydrogel and the re-cellularized hydrogel in preparation of a material for treating lymphatic tissue defects. By compounding the lymph node acellular matrix and the photo-crosslinkable gelatin dopamine hydrogel, a stent carrier which has natural biological signals, is suitable for a three-dimensional microenvironment and has good mechanical properties and in-situ forming characteristics is constructed, and an ideal platform is provided for field planting, growth and function exertion of ADSCs at a lymphatic tissue defect part; the ADSCs are assisted to play a repairing role in promoting lymphatic vessel regeneration and regulating a local microenvironment, and a novel composite hydrogel stent system adaptive to a cell repairing function is provided for treatment of related diseases such as lymphedema.

The utility model discloses a pair of curved claw separating forceps for endoscopic thyroid surgery. The pair of curved claw separating forceps comprises an operating rod, a movable forceps head, a fixed forceps head and a moving component, the first end of the fixed tong head is connected with the operating rod, the first end of the movable tong head is connected with the first end of the moving assembly, and the second end of the moving assembly is connected with the operating rod. The movable tong head comprises a first fixing rod, a first connecting rod and a first separating rod, the first end of the first fixing rod is connected with the moving assembly, the first end of the first connecting rod is horizontally connected with the second end of the first fixing rod in a bent mode, and the second end of the first connecting rod is vertically connected with the first separating rod in a bent mode. During use, the fixed forceps head and the movable forceps head are moved between the esophagus and the trachea, the movable forceps head is moved through the moving assembly so as to increase or decrease the distance between the movable forceps head and the fixed forceps head, fat and lymphatic tissue between the trachea and the esophagus are accurately clamped and separated, and the requirement of a cervical side thyroid surgery is met; and the operation efficiency is improved.

The utility model discloses a multifunctional lifting pad for auxiliary exercise of double lower limbs, which belongs to the technical field of medical instruments and comprises a lifting pad body, a massage portion is arranged on the upper end face of the lifting pad body, and an exercise portion is fixedly arranged at the higher end of the lifting pad body. The exercise part comprises a support, a plurality of pedals and springs, the support is arranged at the higher end of the lifting pad body, the pedals are arranged on the support, and the ends, close to the lifting pad body, of the pedals are connected with the support through the springs; the massage part comprises a massage cushion, the massage cushion is laid on the lifting cushion main body, an inflation air bag is arranged below the massage cushion, the inflation air bag is provided with an inflation valve, and the inflation valve is connected with a pressure pump through an inflation hose. By arranging the inflatable air bag, repeated inflation and deflation can be achieved, and flowing of blood and lymphatic tissue of the two lower limbs can be promoted; the foot pedal can effectively prevent the foot bottom from drooping and is connected with the spring, so that the patient can do ankle pump movement by himself / herself, and the effect of exercising the lower limb function is achieved.

The invention relates to a lymphatic tissue targeted composite nanocluster as well as a preparation method and application thereof. The composite nanocluster comprises Se-doped ZnS nanoparticles and BSA (Bovine Serum Albumin) coated on the surfaces of the nanoparticles. The nano platform for synergistically targeting lymphatic tissues integrates the complementary immunoregulation functions of zinc and selenium, overcomes the curative effect limitation of a single element through the synergistic effect of elements, and widens the safe treatment window of necessary trace elements; the natural lymph targeting characteristic of the serum albumin is utilized to realize efficient lymph node delivery of the nanocluster; meanwhile, the nano-cluster structure can promote in-vivo removal, so that long-term accumulation is reduced, and the safety is ensured.

This disclosure relates to JAK1 pathway inhibitors and the use thereof in treating cytokine-related diseases or disorders such as cytokine release syndrome (CRS), hemophagocytic lymphohistiocytosis (HLH), macrophage activation syndrome (MAS), and CAR-T-cell-related encephalopathy syndrome (CRES).

The invention discloses a nasal delivery double-chain RNA / Mn < 2 + > nano-drug as well as a preparation method and application thereof in immunotherapy. And adding the polymer into a solution containing double-stranded RNA and manganese salt, and self-assembling to form the nasal delivery double-stranded RNA / Mn < 2 + > nano-drug. The invention provides a new technical scheme for the barrier of the BBB. The nano delivery system has the advantages in the aspects of overcoming the BBB and improving the in-vivo biological distribution of the drug, and the surface of the nano carrier is modified with a ligand targeted to an endothelial cell overexpression receptor, such as ApoE peptide and the like, so that the drug can be delivered to penetrate through the BBB; a non-invasive nasal-brain delivery strategy becomes a new scheme for improving drug availability and reducing toxic and side effects, the drug can bypass BBB and rapidly enter brain parenchyma through olfactory bulb and trigeminal nerve conduction pathways, and an immune drug delivered by the nasal cavity can rapidly enter nasopharynx-related lymphatic tissue (NALT) and is transferred to CLN through a lymphatic network around the nasal cavity, so that the immune drug can be rapidly delivered to the nasopharynx-related lymphatic tissue (NALT). Further, a potent local immune response aiming at the in-situ GBM is induced.