Preparation method of lymph node tissue slice

Abstract

The invention discloses a preparation method of lymph node tissues slices. Adipose tissues are attached around and / or inside the lymph node tissues. The method comprises the following steps of: fixing, dehydrating, transparenting, wax soaking, embedding, slicing, and staining the lymph node tissues, wherein a step of treating the lymph node tissues with an alkaline solution is also included between the step of fixing and the step of dehydrating. According to the preparation method of the lymph node tissues slices, the lymph node tissues are treated with the alkaline solution to remove lipid droplets in fat cells, so that the adipose tissue and the lymphatic tissue achieve the same dewatering effect, and thus the whole continuous piece is sliced. In addition, using the method for treating the adipogenic area in the lymph nodes, a void phenomenon can be generated when slicing to obtain high-quality slices.

Description

technical field [0001] The invention belongs to the technical field of preparation of pathological tissue slices, and in particular relates to a preparation method of lymph node tissue slices. Background technique [0002] Pathological examination of lymph nodes in cancer patients is one of the routine tasks of surgical pathology. A large number of studies have shown that the presence of lymph node metastasis is closely related to the prognosis of cancer patients, but the current misdiagnosis rate of lymph node pathological examination is as high as 10%-30%, mainly due to incomplete lymph node sections. Due to the different dehydration effects of peripheral fat or adipose metaplasia in lymph nodes and lymphoid tissue, the hardness of lymph nodes and adipose tissue after dehydration is inconsistent, and slices cannot be completely sliced. [0003] When making lymph node tissue slices, the fat around the lymph nodes has a great influence on the slice production. The fat tissu...

AI Technical Summary

Problems solved by technology

A large number of studies have shown that whether there is cancer metastasis in lymph nodes is closely related to the prognosis of cancer patients, but the current misdiagnosis rate of lymph node pathological examination is as high as 10%-30%, the main reason is that lymph node sections are incomplete

Due to the difference in the dehydration effect between the peripheral fat or the fat metaplasia area inside the lymph node and the lymph tissue, the hardness of the lymph node and fat tissue after dehydration is inconsistent, and it cannot be sliced ​​completely

[0003] When making lymph node tissue slices, the fat around the lymph nodes has a great influence on the slice production. The fat tissue will prevent the reagents from fully contacting the lymph nodes, resulting in lymph node fixation and insufficient dehydration. Moreover, the presence of lipid droplets in fat cells also makes the fat tissue itself fixed and stable. Insufficient dehydration

Fat removal is not only time-consuming, but also may destroy the anatomical integrity of lymph node tissue or cause the loss of smaller lymph nodes, so that it is impossible to accurately judge the number of lymph nodes and whether there is tumor cell invasion in the surrounding fat and blood vessels, and a large amount of valuable information will be lost. clinical information

The relevant pathological techniques reported so far have not been able to solve the above clinical problems well.

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