A kind of preparation method of lymph node tissue slice

A production method and tissue sectioning technology, applied in the field of lymph node tissue section production, can solve the problems of time-consuming fat removal, incomplete lymph node sectioning, destruction of the anatomical structural integrity of lymph node tissue, etc., so as to reduce the workload of pathologists and technicians. , accurate pathological diagnosis, and the effect of improving clinical information
CN109580306BActive Publication Date: 2021-05-11CENT SOUTH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CENT SOUTH UNIV
Publication Date
2021-05-11

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Abstract

The invention discloses a method for making lymph node tissue slices, wherein adipose tissue is attached around and / or inside the lymph node tissue. The following steps are also included between fixation and dehydration: the lymph node tissue is treated with alkaline solution. The present invention uses an alkaline solution to treat the lymph node tissue, which can remove fat droplets in the fat cells, so that the fat tissue and lymph tissue can achieve the same dehydration effect, thereby forming a complete and continuous sheet. In addition, by using the method of the present invention to treat the fat metaplasia area inside the lymph node, the "cavity" phenomenon can be avoided during sectioning, so as to obtain high-quality sections.
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Description

technical field

[0001] The invention belongs to the technical field of preparation of pathological tissue slices, and in particular relates to a preparation method of lymph node tissue slices. Background technique

[0002] Pathological examination of lymph nodes in cancer patients is one of the routine tasks of surgical pathology. A large number of studies have shown that the presence of lymph node metastasis is closely related to the prognosis of cancer patients, but the current misdiagnosis rate of lymph node pathological examination is as high as 10%-30%, mainly due to incomplete lymph node sections. Due to the different dehydration effects of peripheral fat or adipose metaplasia in lymph nodes and lymphoid tissue, the hardness of lymph nodes and adipose tissue after dehydration is inconsistent, and slices cannot be completely sliced.

[0003] When making lymph node tissue slices, the fat around the lymph nodes has a great influence on the slice production. The fat tissu...

Claims

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