Hematoxylin-eosin one-step staining method

A technology of hematoxylin and staining method, which is applied in the field of hematoxylin-eosin staining solution and its staining, can solve the problems of difficulty in grasping the degree of staining of the nucleus and cytoplasm, shorten the operation time, etc., and achieves good staining effect, easy observation and analysis, and stability. Good results

Active Publication Date: 2019-01-22
杨海玉
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  • Claims
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Problems solved by technology

[0006] The present invention is improved on the basis of the conventional HE staining method, and realizes one-step staining of the nucleus and cytoplasm, which not only simplifies the operation steps and shortens the operation time, but also solves the problem that the degree of staining of the nucleus and cytoplasm in conventional HE staining is difficult to grasp.

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  • Hematoxylin-eosin one-step staining method
  • Hematoxylin-eosin one-step staining method
  • Hematoxylin-eosin one-step staining method

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Embodiment Construction

[0019] Taking conventional formalin-fixed and paraffin-embedded pathological tissue sections as an example, conventional HE staining and the operation steps of the present invention are introduced as follows.

[0020] Operation steps of conventional HE staining method.

[0021] 1. Dewax the slices to water.

[0022] ①Two times of xylene, 5-10 minutes each time.

[0023] ②The effect of absolute ethanol is applied twice, each time for 3-5 minutes.

[0024] ③ 95% ethanol for 3 to 5 minutes.

[0025] ④ 90% ethanol for 3 to 5 minutes.

[0026] ⑤ 80% ethanol for 3 to 5 minutes.

[0027] ⑥ Rinse with tap water or distilled water for 1-3 minutes.

[0028] 2. HE staining.

[0029] ① Stain with modified Lillie-Mayer hematoxylin staining solution for 1-5 minutes.

[0030] ②Rinse with tap water or distilled water for 10-30 seconds.

[0031] ③ 1% hydrochloric acid ethanol differentiation 2 ~ 5s.

[0032] ④Rinse with tap water or distilled water for 10-30 seconds.

[0033] ⑤ Weak ...

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Abstract

The invention belongs to a routine dyeing reagent for a routine pathological section making and a use method of the routine dyeing reagent, and discloses a hematoxylin-eosin one-step dyeing method. The hematoxylin-eosin one-step dyeing method disclosed by the invention is characterized by comprising the following steps: preparing a hematoxylin-eosin one-step dyeing solution and a specific pH value buffer solution so that hematoxylin dyeing and eosin dyeing are completed in one step without the steps of differentiating and turning blue, and rinsing the sections with the specific pH value buffer solution before and after dyeing. According to the hematoxylin-eosin one-step dyeing method disclosed by the invention, one-step dyeing of cytoplasm and nucleus can be achieved, the operation steps are simplified and the operation time is shortened; the hematoxylin-eosin one-step dyeing method is good in dyeing effects and stable in results, is suitable for dyeing of routine formalin fixation and paraffin-embedded tissue sections, and can also be used for dyeing of frozen tissue sections or cultured cells, and favorable conditions are provided for rapid and accurate clinical pathological diagnosis.

Description

technical field [0001] The invention relates to a hematoxylin-eosin staining solution and a staining method thereof, which belong to conventional staining reagents for routine pathological film production and methods for using the reagents. Background technique [0002] Hematoxylin-eosin staining (HE staining for short) is the most basic and important staining method in routine pathological slide preparation, and is widely used clinically. Hematoxylin is a basic dye that can stain the nucleus blue; eosin is an acid dye that can stain the cytoplasm red. In pathological diagnosis, teaching and scientific research, HE staining is often used to observe the morphology and structure of normal and diseased tissues. To determine or identify certain abnormal substances and special components in diseased tissues and cells, special staining methods, enzyme histochemical methods or immunohistochemical methods are required, which are also based on the observation of HE-stained tissue se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 刘勇杨海玉
Owner 杨海玉
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