The invention relates to a water pressure resistance test device and test method for seam sealing glue. The device comprises a test table, a concrete sample layer, a fixed frame, a transparent pressure barrel, a water injection pressurizing device, and a counting cup, wherein the bottom of the transparent pressure barrel is provided with an exhaust valve communicated with the inner cavity of the transparent pressure barrel, the water injection pressurizing device comprises a booster pump and a water convey pipe, a water injection valve is installed in the center of the bottom of the transparent pressure barrel, the concrete sample layer comprises two rectangular concrete plates and a sealing glue layer between the two concrete plates, the fixed frame comprises a bottom plate, the center ofthe bottom plate is provided with a rectangular through hole, two ends of the through hole are separately provided with a limit block, two sides of the bottom plate are provided with a block plate separately, and the center of the surface of the test table is provided with an observation port. The water pressure resistance test device for the seam sealing glue has advantages of simple structure,repeated use for tests, low cost, high operability, and high accuracy of test results.