62 results about "Sargassum horneri" patented technology

The invention relates to a cultivation method of economic seaweed, in particular to a full-artificial seed cultivation method for sargassum horneri. Concretely, the method comprises the following steps: selecting male and female sargassum horneri parent strains with good pyrenium growth to be cultivated for 10 to 20 days under the conditions that the illumination intensity is between 2500 and 10000 luxes and the temperature is between 10 and 16 DEG C for realizing the synchronous sexual development and sexual maturation of the sargassum horneri so that ova and sperms are discharged synchronously for impregnation; completing the collection and the seedling distribution of germ cells in 10 to 24 hours after the impregnation; carrying out static cultivation for 2 to 4 days after the seedling distribution; and maintaining the water flowing under the condition of the illumination intensity between 400 and 8000 luxes for completing the cultivation. The seed cultivation process of the invention is carried out in an artificial cultivation system, so the impregnation rate can be further and greatly improved, the spatfall efficiency and the full-artificial seed cultivation efficiency can be improved.

The invention belongs to the field of cosmetics and particularly relates to a sargassum horneri crude polysaccharide skin cream and a preparation method thereof. The sargassum horneri crude polysaccharide skin cream comprises the following raw materials in percentage by weight: 5-10 percent of Sargassum horneri crude polysaccharide, 8-12 percent of humectant, 0.5-1 percent of preservative, 0.5-1 percent of antioxidant, 2-4 percent of nutrition additive, 10-15 percent of oily raw materials, 1-2 percent of thickener, 0.2-0.4 percent of metal ion chelating agent, 0.2-0.4 percent of neutralizer, 1-2 percent of brightening agent, 0.05-0.1 percent of antisensitive agent, 0.3-0.5 percent of rose essential oil and the balance of deionized water. Tests prove that the skin cream has good total antioxidant capacity; after the skin cream is used for three days, the moisture content of the skin is increased by more than 230 percent, the collagen fiber content of the skin is increased by more than 78 percent, and the skin elasticity is improved by more than 235 percent. The sargassum horneri crude polysaccharide skin cream is a skin care product which is low in cost and has a remarkable beautifying effect.

The invention belonging to the field of cosmetics, and particularly relates to a Sargassum horneri boiled alga clay mask and a preparation method thereof. The preparation method comprises the following steps: removing impurities from Sargassum horneri, washing, drying until the algae become embrittled, stamping the dried Sargassum horneri to granules, immersing in alcohol for 8-20 hours, filtering, boiling for 15-120 minutes, filtering to obtain alga clay, carrying out centrifugal dehydration and freeze-drying, and grinding into powder; weighing raw materials according to the formula; adding deionized water into the weighed Sargassum horneri powder, and stirring to swell; and dissolving a moisturizer, an antioxidant, a metallic ion chelator, a preservative, a whitener, a deodorizer, a sebum inhibitor, a cutin dissolving agent, a bacteriostatic agent, an antiallergic agent and a nutrient additive in deionized water, adding the Sargassum horneri clay, continuing stirring while adding a tackifying agent and deionized water, and stirring uniformly. The mask has the advantages of favorable moisturizing property, low cost and low price, obviously improves the skin color, moisturizes the skin, and prevents the skin from aging.

The invention discloses a method for extracting a fucoxanthine crude product from sargassum horneri; the method mainly comprises the steps of pretreatment, suction filtration, ultrasonic leaching, extraction and the like. With the sargassum horneri as a raw material, the fucoxanthine is extracted, the whole extraction process is optimized, the process steps are simple, the operability is strong, the yield of the crude product is high, a theoretical basis is provided for industrialized production for extracting fucoxanthine from the sargassum horneri, and the method has great value for development.

The invention discloses a preparation method of sargassum horneri matrix activated carbon. The preparation method comprises the following steps: (1), mixing sargassum horneri powder and activator with water for impregnating treatment for 2-24 h; (2), placing the mixture I under an inert atmosphere, raising the temperature of the mixture I to 400-600 DEG C according to a rate of 10-30 DEG C/min; performing activating treatment on the mixture I for 1-3 h; then washing the mixture I with hot acid solution and hot distilled water in sequence until the pH value of the mixture does not change; drying the mixture I to obtain a product A; (3), mixing the product A with boiling oxidant solution to enable the product A to react with the boiling oxidant solution for 1-3 h; then, washing the mixture II with hot distilled water until the pH value of the mixture II does not change; drying the mixture II to obtain a product B; (4), performing high-heat treatment on the product B at 300-500 DEG C, so as to obtain the sargassum horneri matrix activated carbon. The activated carbon prepared through the preparation method has the advantages that most pores are mesoporous, the pores are well-developed, the ash content is low, and the adsorbability to hexavalent chromium is higher.

The invention discloses a sargassum horneri beta-sitosterol compound and an extracting method thereof. The sargassum horneri beta-sitosterol compound has remarkable anti-depression effect, and animal experiments in an embodiment show that beta-sitosterol can remarkably reduce dead time of mice in a mouse forced swimming test and a tail suspension test, takes effect quickly, is small in toxic and side effects (TD50 is greater than 2,000 mg/kg), and can be used for a long time, and pharmacological properties of the sargassum horneri beta-sitosterol compound which has high value and serves as anti-depression drug are shown. The beta-sitosterol compound is abundant in source of raw materials and is low in price, the extracting method is simple, and the beta-sitosterol compound can be prepared into anti-depression drugs in various dosage forms.

The invention discloses a scaled cultivation method of sargassum horneri based on nutrient rapid propagation and belongs to the technical field of seaweed breeding. The method comprises the following steps of: 1) selecting a cultivation sea region; 2) collecting sargassum horneri main weeds and transporting and collecting floating sargassum horneri and conveying the same to the cultivation sea region; 3) gripping and sea cultivation: gripping the sargassum horneri on seedling ropes and hanging the seedling ropes on a raft rack; 4) daily management and harvesting: selecting the main weeds, then performing cultivation and harvesting; 5) main weed conservation and aestivation: continuously breeding and aestivating the main weeds in a region which is deep, large in flow and low in temperature; and 6) planting and cultivation of the main weeds again: continuously collecting the main weeds, gripping the same, and cultivating the same to obtain the main weeds and harvesting the sargassum horneri. The method for vegetative propagation by means of nutritional branches of the floating sargassum horneri has the characteristic of yearly growth, sargassum horneri can be cultivated on a large scale conveniently and rapidly, and the method is simple to operate, time- and labor-saving and high in growing speed, is not limited by manure main weeds, a lot of main weeds can be cultivated anytime, a phenomenon of seedling elimination is avoided, and the survival rate is high.

The invention relates to the artificial breeding technique of sargassum horneri, belonging to the technical field of the fingerlings of algae. The artificial breeding is implemented by the four stages of seedling collection, indoor cultivation, artificial seeding and indoor culture. The invention has the advantages of easy mastery of the technique, small investment simple facilities and other advantages.

The invention discloses an alkaloid compound as well as a preparation method and an application of the alkaloid compound. The alkaloid compound has a molecular formula of C15H21NO4. The preparation method of the alkaloid compound comprises the steps that activated Pestalotiopsis sp. fungi Z233 are inoculated into culture liquid, and the fermentation culture is carried out for 10 to 40 days at 20 to 30 DEG C; mycelia and fermentation liquid are obtained through separation after the fermentation culture; after the mycelia are leached, extracted and concentrated by organic solvents, mycelium extractives are obtained; or the fermentation liquid is extracted and concentrated to obtain fermentation liquid extractives; and the chromatographic separation and the purification treatment are carried out on the mycelium extractives or the fermentation liquid extractives, and the alkaloid compound is prepared. The alkaloid compound with a novel structure is extracted and separated from fermentation culture substances of Sargassum horneri epiphytic Pestalotiopsis sp. fungi Z233, and the alkaloid compound has better antitumor activity, can be used for preparing antitumor medicine and tumor prevention health-care food and has good development prospects.

The invention aims at providing a sargassum horneri extract for promoting seed germination and application of the sargassum horneri extract. A preparation method of the sargassum horneri extract comprises the following steps: picking airbags of fresh sargassum horneri, cleaning, mashing and then adding a methanol water solvent; extracting under the condition of room temperature, then carrying outfirst-time vacuum concentration to a certain alcohol concentration under the low-temperature condition of 25 to 35 DEG C, standing and crystallizing; carrying out second-time vacuum concentration on residual solution to a certain alcohol concentration, standing and crystallizing; finally, carrying out aqueous phase concentration to obtain the sargassum horneri extract. The sargassum horneri extract provided by the invention is an environment-friendly safe biological agent. The extract provided by the invention is used for promoting germination of plant seeds and is wide in application range. In addition, the sargassum horneri extract comprehensively utilizes the current sargassum horneri causing algal bloom.

The invention discloses a suspension cultivation method for sargassum horneri seedlings. The method comprises the following steps: selecting the sargassum horneri seedlings of 50-60 days without attached matrixes and placing in a cultivation jar in a cultivation room, wherein the density of the seedlings is 15-25 plants/L; continuously inflating in a cultivation period; keeping the sargassum horneri seedlings in a suspension state all the time; changing 50 percent of water each day by using artificially purified seawater; artificially regulating and controlling an environmental factor and proper cultivation density in the whole process; cultivating for 20-35 days; cultivating the sargassum horneri seedlings in the sea when the plant height of the sargassum horneri seedlings is 5-7 cm. According to the method, the attached matrixes are saved, the adhesion influence of enemy fishes, diatom, dirt and the like is avoided, and the seedling cultivation rate is improved.

The invention discloses an enzymolysis-modified sargassum horneri polysaccharide and an application thereof. A preparation method of the enzymolysis-modified sargassum horneri polysaccharide comprises steps as follows: (1) performing microwave extraction on a sargassum horneri polysaccharide to obtain a coarse polysaccharide extract; (2) performing grading alcohol precipitation: performing precipitation on the coarse polysaccharide extract with the final ethanol volume fraction of 30-40% and 55-65% successively to obtain an SHP60 coarse polysaccharide component; (3) performing decolorization and deproteinization on the SHP60 coarse polysaccharide component to obtain an SHPA polysaccharide; (4) performing enzymolysis on the SHPA polysaccharide with a dextranase to obtain an enzymatic product; (5) performing ion column chromatography on the enzymatic product with a DEAE-Sepharose Fast Flow ion column chromatography system, and concentrating and filtering an eluent to obtain a filtrate; (6) performing gel column chromatography on the filtrate obtained in Step (5) with a Sephadex G-25 gel chromatography system, collecting a salt-free polysaccharide part, and performing concentration and freeze-drying to obtain the enzymolysis-modified sargassum horneri polysaccharide. The enzymolysis-modified sargassum horneri polysaccharide can be applied to preparation of an antioxidant, a moisture absorbent and a moisturizer.

The invention relates to Lutjanus stellatus feed and a preparing method of the Lutjanus stellatus feed. The Lutjanus stellatus feed comprises protein source raw materials, fat source raw materials, sugar source raw materials and auxiliary materials, and is characterized by also comprising 5 weight percent of Sargassum horneri powder, wherein the protein source raw materials are fish meal; the fat source raw materials are selected from fish oil, bean oil and a composition formed by a mixture of the fish oil and the bean oil; the sugar source raw materials are cassava starch; the auxiliary materials are selected from complex vitamins, mixed mineral substances, microcrystalline cellulose, soybean phosphatide powder, DL-methionine, calcium biphosphate, choline chloride, ascorbic acid, sleeve-fish paste and a composition formed by a mixture of the complex vitamins, the mixed mineral substances, the microcrystalline cellulose, the soybean phosphatide powder, the DL-methionine, the calcium biphosphate, the choline chloride, the ascorbic acid and the sleeve-fish paste; and the feed consists of the following nutritional ingredients in percentage by weight: 41.23 to 41.72 percent of crude protein, 8.32 to 8.52 percent of crude fat and the balance being the carbohydrates, crude fiber, ash and water. The Lutjanus stellatus feed has the advantages that the weight increase rate and the survival rate of the Lutjanus stellatus are higher; in addition, the consumption of the fish meal can be reduced; and the protein demand level of fingerlings can be reduced.

The invention relates to a preparation method of an adsorbent for adsorbing heavy metal ions. The preparation method comprises the following steps: (1) air drying, cleaning, drying and crushing raw material Sargassum horneri; and (2) introducing saturated steam into Sargassum horneri particles to reach an assigned pressure, maintaining for a certain time, and instantaneously relieving the pressure to a normal pressure; and (3) carrying out heating reflux by virtue of formaldehyde-sulfuric acid mixed liquid, filtering, flushing filter residues with deionized water, and drying to constant weight, so as to obtain the adsorbent. The preparation method has the beneficial effects that the raw materials have a wide source, is cheap and easily available, the removal rate of a typical heavy metal pollutant, namely chromium ions in water is relatively high, the separation, recycling and reutilization are convenient, the secondary pollution is avoided, the reasonable utilization of resources is realized, and the economic and environmental benefits are good. The preparation method has wide application prospects in sewage treatment.

The invention relates to a sargassum horneri cold preservation closet and a transport preservation method, and belongs to the field of alga cold-chain logistics. In order to overcome the defects in the prior art, the sargassum horneri cold preservation closet and the transport preservation method are provided. According to the cold preservation closet, ice boxes filled with refrigerant are evenly arranged inside a closet body; and a water pervious device and a water collection device are arranged on the lower portion of the closet body, sargassum horneri leachate can be discharged in time, and meanwhile excessive water loss of sargassum horneri can be prevented. Compared with a traditional transport preservation method, frond preservation time can be effectively prolonged, and the frond preservation effect is improved.

An extraction method for sargassum horneri polyphenol is characterized by comprising: A, taking sargassum horneri as a raw material, crushing sargassum horneri, and sieving by using a 25-35 mesh sieve, so as to obtain a uniform sargassum horneri powder; B, accurately weighing a certain amount of sargassum horneri powder, adding an organic solvent aqueous solution with the concentration of 40%-90% according to the material-solution ratio of 1:9-11 (w/v), and fully uniformly mixing and immersing; C, immersing for 10-15 min, and using a supersonic-wave cell crushing machine to crush for 5-10 min to perform extraction of polyphenol; D, performing centrifugal processing on the extraction liquid, and controlling the processing technology of 3500-4500 rpm and 4-6 min; E, repeatedly extracting for 4-6 times, and merging the supernatants; and F, performing 45 DEG C evaporation concentration on the supernatant by a rotary evaporator, and then performing vacuum freeze drying, so as to obtain the sargassum horneri polyphenol extractive. The extraction method overcomes long time, high temperature, low extraction rate and other adverse effects of a conventional method of using water to extract polyphenol, and is relatively simple and time-saving and low in cost, and the extracted sargassum horneri polyphenol product is high in content and relatively strong in cell antioxidation performance.

The invention relates to a sargassum horneri spore collecting device and a use method thereof. The device comprises a raw material barrel of which the inner cavity is internally provided with a first mesh bag, the inner cavity of the first mesh bag is internally provided with a second mesh bag, the opening part of the raw material barrel is connected with the bottom by a pipeline, a collecting barrel is arranged below the pipeline and is internally provided with a third mesh bag, the upper opening part of the third mesh bag is connected to the pipeline below the bottom of the raw material barrel, a part, extending into the raw material barrel, of the pipeline is provided with a sprayer, the pipeline at the opening part of the raw material barrel is provided with a first valve, a second valve and a third valve are respectively arranged at two sides of a connection part of the third mesh bag and the pipeline, on the pipeline below the raw material barrel, and the collecting barrel is provided with a drainage port at which a fourth valve is arranged. According to the sargassum horneri spore collecting device and the use method thereof, a method of filtering and flushing by the multiple layers of mesh bags is adopted, so that the yield rate of sargassum horneri spores is improved, the quantity of impurities is lowered, the adhesive force is strengthened, and the effects of centralized collecting and uniform spraying and transplanting are achieved.

The invention discloses a tobacco processing method. By adding the main substance cinnamic aldehyde in cinnamon essential oil to improve the sensual smell of tobacco, the tobacco has the advantages oflasting aroma and no toxicity or harm; in order to overcome the defect that cinnamic aldehyde volatilizes fast, the aqueous extract of sargassum horneri with high moisture retention is added for wrapping to make cinnamic aldehyde attached to the tobacco for a long time, and the addition of the aqueous extract of sargassum horneri effectively improves the processability of the tobacco, enhances the processing resistance and reduces the broken leaf rate; by adding various enzymes in the processing process to accelerate tobacco fermentation, the tobacco which is good in taste, low in pungency and beneficial to human health is obtained. The tobacco processing method is simple and easy to operate, obviously improves the tobacco quality and improves the processing resistance, thereby being worthy of popularization.

The invention relates to cultivation of economic brown seaweed and in particular relates to a method for preserving sargassum horneri fertilized eggs at low temperature. The method comprises the following steps: collecting eggs and sperms, which are synchronously discharged by economic seaweed of sargassum horneri, within 2-3 days after fertilization, and preserving the collected fertilized eggs in seawater at the temperature of 4-8 DEG C under 2000 lux. With the adoption of the method, the development process of the fertilized eggs can be greatly delayed, and trans-regional transport of the fertilized eggs in different places is finished before rhizoid elongation, so that seed production can be carried out.

The invention belongs to the technical field of marine organism natural products and particularly relates to a method for preparing sargassum horneri powder and extracting fucoxanthin from sargassum horneri. The method includes steps: preparing sargassum horneri powder by cleaning, drying in the shade, drying, powdering, sterilizing and the like; adding extract liquid and steel balls into the sargassum horneri powder, performing oscillatory extraction for 3-10 minutes under weak-light, low-temperature and oxygen-free conditions, centrifuging to obtain supernatant, and subjecting the supernatant to high performance liquid chromatography and vacuum freeze drying to obtain a pure fucoxanthin product. The method has advantages of high efficiency, simplicity, low cost and the like, provides basis for biological resource comprehensive utilization of sargassum horneri and has a potential significant economic value.

The invention relates to a technology for repairing nuclide pollution, in particular to a method for using living seaweed to repair seawater nuclide strontium pollution. According to the method, living Saccharina japonica or Sargassum horneri are used to repair the pollution of nuclide strontium in seawater. The method has the advantages of good adsorption effect, low cost and simple and convenient operation, and laying an important foundation for in situ bioremediation of near-shore radionuclide-contaminated sea areas.

The invention relates to a composite active carbon material and a preparation method thereof. The composite active carbon material is prepared from the following raw materials in percentage by mass: 50-60% of powdery active carbon, 25-35% of active carbon fiber, 10-15% of chopped fiber and 2-5% of arcylic glue, wherein the powdery active carbon is sargassum horneri active carbon, the length of thechopped fiber is 35-50 mm, and the total weight percentage content of the components is 100%. The preparation method comprises the steps that active carbon fiber, sargassum horneri active carbon, chopped fiber and acrylic glue are weighed, a mixed raw material is obtained, and the mixed raw material and water are uniformly mixed; the mixture is produced to be net and sized on a paper making machine at a time, and the active carbon fiber composite material is formed. The composite active carbon material has the enlarged specific surface area, high adsorption capability and wide range of organic matter adsorption, and also has the good adsorption and purification ability on wastewater containing Cr (VI). The preparation method of the composite active carbon material is simple and practical,can be applied to large-scale production, and can be used in the field of treatment of wastewater.

The present invention relates to a medium composition for the dedifferentiation of induced pluripotency stem cells, containing a phlorotannin fraction extracted and isolated from one type of brown algae selected from the group consisting of Ecklonia cava, Dictyopteris prolifera, Dictyota coriacea, Sargassum horneri, Ishige okamurai and the like. In addition, the present invention relates to a method for preparing induced pluripotency stem cells by using the medium composition. Induced pluripotency stem cells can be safely, easily and effectively prepared by using mesenchymal stem cells by using the medium composition of the present invention, and the prepared induced pluripotency stem cells can be differentiated into various cells, and thus can be useful as a cell therapeutic agent.