Full-artificial seed cultivation method of sargassum horneri

A cultivation method, copper algae technology, applied in the field of copper algae artificial seedling cultivation, can solve the problems of low seedling raising efficiency, low parental fertilization rate, and small number of offspring, etc., to improve the efficiency of seedling attachment, the efficiency of raising seedlings, and the improvement of cultivation efficiency effect

Inactive Publication Date: 2010-06-30
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After a certain period of time, the natural shedding of fertilized eggs leads to a small number of offspring, or misses the best time for seedling collection
In addition, the asynchrony of fertilization leads to different developmental stages of offspring, which makes it easy to shed seedlings in the later stage; the biological characteristics of dioeciousness determine that the fertilization rate of the parents of these two sources is low, and the development of offspring embryos and fertilized eggs is asynchronous, resulting in the efficiency of seedling raising low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1) Simultaneous discharge of eggs and sperm: select male and female parents of copper algae with well-developed reproductive receptacles and culture them for 20 days at a light intensity of 2500 lux and a temperature of 12°C to achieve synchronous development and maturation of copper algae, so that eggs and sperm are released simultaneously. Fertilization; the ratio of the male and female parents is 10:1 (adding nutrient salt NaNO 3 : 5ppm; KH 2 PO 4 : 0.3ppm).

[0014] 2) Seed cultivation: within 24 hours after fertilization, the collection of fertilized eggs and seedling distribution are completed. The fertilized eggs are collected by natural settlement and stirring, and seedlings are distributed according to the density of 3-4 fertilized eggs per square centimeter After the seedlings were placed, they were cultured statically for 48 hours, and the seedlings were inoculated and attached to the attachment base for 2 days and under the light of 4000 lux, and then the ...

Embodiment 2

[0016] 1) Simultaneous discharge of eggs and sperm: the male and female parents of copper algae with well-developed reproductive receptacles are selected and cultured for 10 days at a light intensity of 10,000 lux and a temperature of 15°C to realize the simultaneous development and maturation of copper algae, so that eggs and sperm are released simultaneously. Carry out fertilization; The ratio of the male and female parents is 20:1 (add nutrient salt NaNO during the period 3 : 10ppm; KH 2 PO 4 : 1ppm).

[0017] 2) Seed cultivation: within 24 hours after fertilization, the collection of fertilized eggs and seedling distribution are completed. The fertilized eggs are collected by natural settlement and stirring, and seedlings are distributed according to the density of 3-4 fertilized eggs per square centimeter After planting the seedlings, cultivate them statically for 4 days, inoculate the seedlings on the attachment base, and attach the seedlings for 4 days under the light...

Embodiment 3

[0019] 1) Simultaneous discharge of eggs and sperm: select the male and female parents of copper algae with well-developed reproductive receptacles and culture them for 15 days at a light intensity of 5000-7500 lux and a temperature of 16°C to realize the synchronized development and maturation of copper algae, so that eggs and sperm are synchronized discharge, and fertilize; the ratio of the male and female parents is 15:2 (during adding nutrient salt NaNO 3 : 1ppm; KH 2 PO 4 : 0.1ppm).

[0020] 2) Seed cultivation: Within 24 hours after fertilization, complete the collection of fertilized eggs and cloth seedlings. The fertilized eggs are collected by natural settlement and stirring, and the seedlings are distributed according to the density of 5-6 fertilized eggs per square centimeter. After the seedlings were cultured statically for 3 days, the seedlings were inoculated on the attachment base, and the seedlings were attached for 3 days under the light of 8000 lux, and the...

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Abstract

The invention relates to a cultivation method of economic seaweed, in particular to a full-artificial seed cultivation method for sargassum horneri. Concretely, the method comprises the following steps: selecting male and female sargassum horneri parent strains with good pyrenium growth to be cultivated for 10 to 20 days under the conditions that the illumination intensity is between 2500 and 10000 luxes and the temperature is between 10 and 16 DEG C for realizing the synchronous sexual development and sexual maturation of the sargassum horneri so that ova and sperms are discharged synchronously for impregnation; completing the collection and the seedling distribution of germ cells in 10 to 24 hours after the impregnation; carrying out static cultivation for 2 to 4 days after the seedling distribution; and maintaining the water flowing under the condition of the illumination intensity between 400 and 8000 luxes for completing the cultivation. The seed cultivation process of the invention is carried out in an artificial cultivation system, so the impregnation rate can be further and greatly improved, the spatfall efficiency and the full-artificial seed cultivation efficiency can be improved.

Description

technical field [0001] The invention relates to a method for cultivating economic seaweed, in particular to a method for cultivating copper algae artificial seedlings. Background technique [0002] The Latin name of copper algae is Sargassum horneri. It is an important economic seaweed in my country's coastal areas, especially in subtropical coastal areas. It is an important raw material for alginate and an important part of aquatic animal feed. At present, the artificial copper algae seedling technology is to use the wild vegetables grown naturally in the intertidal zone, or the individuals cultivated on the seedling rope as the parents. When raising the seedlings, the ovulation time of the parents is judged according to experience, and the fertilized eggs are collected. After exceeding a certain period of time, the natural shedding of fertilized eggs results in a very small number of offspring, or the best time for picking seedlings is missed at all. In addition, the asyn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 逄少军高素芹
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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