Sargassum horneri extract for promoting seed germination

An extract and copper algae technology, applied in the application field of seaweed extract, can solve the problems of soil damage, crop yield and quality decline, affecting crop growth and development, etc., and achieve the effect of promoting germination and being widely used.

Inactive Publication Date: 2018-10-09
陈奥陶
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, with the gradual deterioration of the global climate and environment, various diseases and insect pests, drought, high and low temperature stress and other problems have become increasingl...

Method used

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  • Sargassum horneri extract for promoting seed germination
  • Sargassum horneri extract for promoting seed germination
  • Sargassum horneri extract for promoting seed germination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The applicant first grinds different parts of copper algae, and then extracts them with methanol-water-formic acid (15:4:1, v / v / v). The extract is germinated with cucumber seeds. Each group has 6 seeds, and the seeds are respectively After soaking for 30 minutes with extracts from different parts, put them into a germination bag. The copper algae extract solution was continuously added to the bag, and clear water was used as a negative control group. As a result, it was found that the air sac part had the best germination effect on cucumber seeds. One seed germinated at 48 hours, and at 60 hours, the germination rate reached 4, which were all higher than the extracts from the other two copper algae parts (Table 1); Therefore, copper algae air sacs were selected as the extracted material.

[0020] Table 1: Comparison table of the germination rate of different parts of copper algae extracts on seeds

[0021]

Embodiment 2

[0022] The preferred of embodiment 2 extraction method

[0023] First use the reported method to extract:

[0024] Fresh copper algae was cleaned and crushed, then methanol water solvent was added, after extraction at room temperature, vacuum concentration was performed at a low temperature of 25-35°C to obtain copper algae extract (extract 1 in Table 2);

[0025] Using the extract obtained by the above method to carry out the germination experiment on the air sac extract to cucumber seeds, it was found that the extract obtained by the above method needs a high concentration to have a good effect; and the rooting effect is not good.

[0026] In order to overcome the above-mentioned problems, the present invention optimizes the extraction method.

[0027] The method established by the present invention is as follows:

[0028] After the fresh copper algae is collected, 200g of the air sac part is separated, and washed 3 times with clean water. Crush to a particle size of 2-3m...

Embodiment 3

[0037] Dilute the air sac extract in the above implementation case 2 to 50, 100, 200 μg / ml with water, select a total of 30 cucumber seeds purchased, 6 seeds in each group, soak the seeds in different concentrations of extracts for 30 minutes, Place in a germination bag. Continue to add copper algae extract solutions of different concentrations into the bag, and use clear water as a negative control group, and commercially purchased common liquid fertilizer as a positive control group.

[0038] Cucumber seeds begin to germinate on day 2, with distinct root hairs beginning to appear on day 3.

[0039] 1) Effect of copper algae extract on germination rate of cucumber seeds

[0040] From the results in Table 3, the cucumber seeds began to germinate on the second day, and the seeds of the clear water group did not germinate on the second day, and the common group did not germinate, but the copper algae extract began to germinate, and the addition amount was the largest The 3rd g...

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Abstract

The invention aims at providing a sargassum horneri extract for promoting seed germination and application of the sargassum horneri extract. A preparation method of the sargassum horneri extract comprises the following steps: picking airbags of fresh sargassum horneri, cleaning, mashing and then adding a methanol water solvent; extracting under the condition of room temperature, then carrying outfirst-time vacuum concentration to a certain alcohol concentration under the low-temperature condition of 25 to 35 DEG C, standing and crystallizing; carrying out second-time vacuum concentration on residual solution to a certain alcohol concentration, standing and crystallizing; finally, carrying out aqueous phase concentration to obtain the sargassum horneri extract. The sargassum horneri extract provided by the invention is an environment-friendly safe biological agent. The extract provided by the invention is used for promoting germination of plant seeds and is wide in application range. In addition, the sargassum horneri extract comprehensively utilizes the current sargassum horneri causing algal bloom.

Description

technical field [0001] The invention belongs to the technical field of seaweed extract application, and in particular relates to a copper algae extract for promoting seed germination. Background technique [0002] In recent years, with the gradual deterioration of the global climate and environment, various diseases and insect pests, drought, high and low temperature stress and other problems have become increasingly serious, and have affected the growth and development of crops, reducing the yield and quality of crops. But now farmers mostly use chemical fertilizers, which are very harmful to people's bodies and can damage the soil. Many countries have begun to vigorously promote the application of organic fertilizers and biological fertilizers to develop green agriculture. At present, the application of seaweed fertilizer in planting and breeding has been applied in many countries. South Africa, the United Kingdom and New Zealand are relatively successful countries in th...

Claims

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Application Information

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IPC IPC(8): A01N65/03A01P21/00A01C1/00
CPCA01C1/00A01N65/00A01N65/03
Inventor 陈奥陶陈海敏
Owner 陈奥陶
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