1439 results about "Biologic Agents" patented technology

The invention relates to the technical field of optical signal processing, in particular to an optical signal processing algorithm based on biological agent fluorescence detection, which comprises the following steps: S1, collecting fluorescence signals with different wavelengths emitted by each probe, and generating a multi-channel fluorescence spectrum data set; s2, carrying out spectral de-mixing on the fluorescence signals in the fluorescence spectrum data set, separating a plurality of overlapped fluorescence signals, and decoupling the fluorescence signals with different wavelengths; s3, for highly overlapped fluorescence signals, carrying out secondary overlapping separation treatment on the fluorescence signals by using a nonlinear optimization method; s4, extracting the intensity, wavelength and time change characteristics of each fluorescent probe signal, and constructing a multi-dimensional fluorescent signal characteristic vector; and S5, by amplifying the characteristic peak value of the weak fluorescence signal, it is ensured that the signal is not covered by strong signals of other probes in multi-probe detection. According to the invention, the resolution of fluorescence detection is improved, so that the high-precision detection effect can be maintained under complex experimental conditions.

The present disclosure provides compositions and methods for treating a disease or condition in a subject in need thereof, comprising administering to the subject a pharmaceutically effective amount of a composition comprising a plurality of particles comprising at least one therapeutic biologic suspended in a pharmaceutically acceptable liquid carrier.

The invention discloses talaromyces purpurogenus, a preparation method and application in prevention and treatment of corn stalk rot, and provides a novel strain, namely talaromyces purpurogenus Jnf41, greenhouse control effect experiments show that the talaromyces purpurogenus Jnf41 can inhibit the scab area and the scab length of the corn stalk rot in greenhouse control effect determination, and the talaromyces purpurogenus Jnf41 can be used for preventing and treating the corn stalk rot. Infection of the corn stalk rot can be effectively inhibited, pathogenic bacteria are not prone to generating drug resistance, green prevention and control are implemented in the prevention and control process, and the biological preparation for resisting the corn stalk rot is prepared and has important significance on green prevention and control of the corn stalk rot.

The invention provides a streptomyces, which is named as Streptomyces sp. And is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 65917. The invention also provides a fermentation broth of the streptomyces, and an ethanol extract and a crude extract of the fermentation broth. The novel streptomyces, the crude extract thereof and the like have broad-spectrum antibacterial activity, have a good antagonistic effect on various pathogenic bacteria such as banana long leaf spot germs and banana colletotrichum gloeosporioides, can enable hyphae of the banana colletotrichum gloeosporioides to shrink, deform and break, inhibit spore germination of the banana colletotrichum gloeosporioides, and have a good anti-bacterial effect on the banana colletotrichum gloeosporioides. According to the present invention, with the application of the bacterial strain, the colletotrichum gloeosporioides spores are shrunk and broken, the growth of the colletotrichum gloeosporioides is inhibited, the peroxidase activity, the polyperoxidase activity, the CAT activity and the like in the banana peel can be improved, and the bacterial strain is the potential biological agent for the prevention and control of the colletotrichum gloeosporioides diseases, and has wide development space and good development and application prospects.

The invention provides bacillus velezensis NR10, and the preservation number of the bacillus velezensis NR10 is CGMCC (China General Microbiological Culture Collection Center) NO.31771. The bacillus velezensis NR10 is a strawberry endophyte, has good bacteriostatic activity, has good antagonistic action on botrytis cinerea, fusarium oxysporum, colletotrichum gloeosporioides, colletotrichum oxysporum, trichoderma harzianum, trichoderma longibrachiatum and the like, is a potential biological preparation for preventing and treating strawberry diseases and the like, and has broad application prospects. And the bacteriostatic action of the bacillus velezensis NR10 has thermal stability. In addition, the bacillus velezensis also has good capacities of producing siderophores, fixing nitrogen and dissolving potassium, producing protease and cellulase and the like. The bacillus velezensis NR10 provided by the invention has wider-spectrum disease resistance, wide development space and good development and application prospects.

The present invention provides: a sludge decomposing bacterium having a 16S rRNA gene comprising a base sequence having 97% or more identity with the base sequence recorded in sequence number 1, a bacterium having a 16S rRNA gene comprising a base sequence having a base variation number of 2 bases or less relative to the base sequence recorded in sequence number 1 and having target microorganism decomposing ability, and a microbial preparation for decomposing a target microorganism comprising the bacterium (a1), wherein the bacterium (a1) is a bacterium having a 16S rRNA gene comprising a base sequence having 90% or more identity with the base sequence recorded in sequence number 1.

The invention belongs to the technical field of environment, and particularly relates to an environment-friendly biological agent for repairing and treating petroleum polluted water and soil and a preparation method of the environment-friendly biological agent. The biological agent is prepared by mixing a compound microbial agent, a biological surfactant and an auxiliary material in parts by mass, has the characteristics of high efficiency, environment friendliness and no secondary pollution, can remarkably improve the remediation efficiency of petroleum-contaminated soil, and has a wide application prospect.

The invention provides application of a turbellarian worm extract in preparation of a product for promoting wound healing, a biological agent for promoting the proliferation activity and / or migration ability of fibroblast NIH3T3, a biological agent for improving the wound healing rate and the decrustation time and a biological agent for improving the collagen fiber deposition type in the wound healing process. The biological preparation is used for promoting regeneration of skin appendage organs in the wound healing process. Wherein the extraction of the turbellarian worm extract comprises the steps of turbellarian worm cleaning, tissue crushing, alcohol extraction, centrifugal separation and concentration drying. The turbellarian worm extract has good capacity of promoting wound healing, and has the effects of effectively improving skin wound healing efficiency, shortening decrustation time, inhibiting scar formation in the wound healing process, promoting regeneration of skin appendage organs in the wound healing process and the like.

The invention discloses a lysosome targeted chimera based on Sortilin and application thereof. The lysosome targeted chimera comprises a ligand molecule of a lysosome targeted receptor and a ligand molecule of a targeted protein to be degraded, wherein the ligand molecule and the ligand molecule are connected through a linker; the lysosome targeting receptor is Sortilin, and the ligand molecule of the lysosome targeting receptor is a polypeptide targeting the Sortilin; the protein to be degraded is membrane protein or extracellular protein. Furthermore, the polypeptide of the targeted Sortilin is connected with an enzyme response sequence and an integrin receptor targeting sequence. According to the invention, the Sortilin-combined ligand neurotensin is functionally coupled with the neutralizing antibody or polypeptide of overexpression protein in targeted tumor or inflammatory diseases, and the Sortilin-combined ligand neurotensin has obvious treatment gain in malignant solid tumor and psoriasis models. The discovery promotes the transformation potential of the lysosome participating in the biological agent, and provides a dual strategy for realizing accurate degradation through receptor recruitment and microenvironment induction.

The invention belongs to the field of biological agents, and particularly relates to bacillus siamensis and application thereof in the aspects of plant disease control, growth promoting and yield increasing. The bacillus siamensis XNC-3 provided by the invention is wide in antibacterial spectrum, can effectively inhibit various plant pathogenic fungi including phytophthora parasitica var nicotianae, has broad-spectrum antibacterial activity, and can be used for preventing and treating plant diseases, especially tobacco diseases. The bacillus siamensis has a promoting effect on tobacco seedling growth and seed germination, has growth-promoting and yield-increasing effects on tobacco, can remarkably increase the tobacco yield and improve the tobacco quality, is safe to the environment and has a very good application prospect.

The invention provides a beauveria bassiana engineering strain Bb-dsMucin for expressing dsRNA (double-stranded ribonucleic acid) as well as a construction method and application of the beauveria bassiana engineering strain Bb-dsMucin. The beauveria bassiana is a beauveria bassiana QSE-F1 strain, and the strain can be colonized at the tomato leaves. The dsRNA can target a botrytis cinerea key gene BcMucin, and the expression of the BcMucin gene is silenced in an RNAi (Ribonucleic Acid Interference) mode. According to the engineering strain, a carrier containing a target gene sequence is transferred into a beauveria bassiana QSE-F1 strain by utilizing an ATMT technology, and the engineering strain Bb-dsMucin capable of synthesizing dsRNA of targeted BcMucin is obtained. The invention also provides an application of the engineering strain Bb-dsMucin, and the application is that the engineering strain Bb-dsMucin can interfere with the expression of a target gene BcMucin, so that the botrytis cinerea can be effectively prevented and treated. Experiments prove that the provided engineering strain Bb-dsMucin can be used for preparing biological agents for preventing and treating gray mold and has a good market application prospect.

The present invention discloses the application of liver cancer cell ferroptosis inducer miR‑11085‑5p in the preparation of liver cancer treatment drugs, and innovative applications in the field of liver cancer treatment, specifically to the application of liver cancer cell ferroptosis inducer miR‑11085‑5p in the preparation of liver cancer treatment drugs. The present invention adopts lentiviral transfection technology to synthesize miR‑11085‑5p in vitro, and introduce it into liver cancer cells, and effectively induce liver cancer cells to undergo ferroptosis by precisely regulating the key signaling pathway of intracellular AMPKα / Nrf2, significantly inhibiting its proliferation activity. Therefore, for the disadvantages of low efficiency, large side effects and easy recurrence and metastasis of existing treatment methods for liver cancer, the miR‑11085‑5p provided by the present invention may become a biological preparation for the treatment of liver cancer, with potential drug development value, and provide a new solution for the treatment of liver cancer.

The invention discloses an application of an MDH2 knockout system or inhibitor in reducing toxicity of T-2 toxin, and belongs to the technical field of biological agents. An MDH2 knockout IPEC-J2 cell line is constructed through a CRISPR / Cas9 gene editing system, and it is found that the cell activity of the cell line is remarkably improved under the condition of 20 ng / mLT-2 toxin concentration, the cell death rate is remarkably reduced, and the cell line has obvious resistance to T-2 toxin. The MDH2 inhibitor LW6 can also enhance the resistance of the cells to T-2 toxin when being used for treating the cells. Therefore, the toxicity of the T-2 toxin in the cell can be reduced by knocking out the MDH2 gene in the IPEC-J2 cell and inhibiting the activity of the MDH2, the tolerance of the cell to the T-2 toxin is improved, and a theoretical basis is provided for prevention and control of harm of the T-2 toxin to human and animals.

The invention provides lipidosome wrapping L-arginine / Prussian blue nano particles and a preparation method and application thereof, and belongs to the technical field of biological agents. L-arginine is deposited on hPB through iron chelation, then the lipidosome is coated to avoid leakage of water-soluble L-arginine in circulation, and thus the L-arginine / Prussian blue nano particles are obtained. The liposome coated with the L-arginine / Prussian blue nano particles is obtained. Due to the EPR effect, the hPLL-coated Lipo can be efficiently transferred and distributed into tumor tissues, heat is generated under the near-infrared light irradiation condition to ablate a large number of tumor cells, the utilization degree of the tumor cells to L-arginine is greatly limited, meanwhile, immunogenic death is induced, DAMPS is released in situ, infiltration and activation of anti-tumor T cells are further promoted, and the anti-tumor effect is achieved. The tumor immunotherapy is enhanced. And under illumination, the liposome disintegrates and releases the L-arginine, so that nutritional support is provided for immunological competence cells in competitive disadvantage, and activation of an immune microenvironment is promoted.

The invention provides a method for intensively removing antibiotic resistance genes in a composting process, and belongs to the technical field of solid waste treatment. The method comprises the following steps: performing aerobic composting on compost materials, and when the temperature in a compost body is reduced to 38-42 DEG C, inoculating a microbial preparation; the microbial preparation is a microbial agent with a function of promoting degradation of organic matters. Based on the principle that a microbial preparation is inoculated in a compost cooling period (38-42 DEG C), a microbial community structure is changed, and ARGs host bacterium proliferation is effectively inhibited, the effect of strengthening removal of resistance genes in the compost process is remarkable, operation is easy and convenient, control and implementation are easy, secondary pollution cannot be caused in the whole process, and the method is suitable for industrial production. The method has a wide prospect of popularization and application in a large range.

The invention belongs to the technical field of biological agents, and particularly discloses application of lily extracellular vesicles in preparation of tumor treatment drugs. The preparation method of the lily extracellular vesicles comprises the following steps: S1, juicing lily bulb tissues, and filtering to obtain a lily bulb stock solution; s2, treating the lily bulb stock solution obtained in the step S1 to obtain a lily extracellular vesicle extracting solution; s3, concentrating and purifying the lily extracellular vesicle extracting solution obtained in the step S2 to obtain lily extracellular vesicles; wherein in the step S2, the treatment comprises centrifugation and filter membrane filtration. The lily extracellular vesicle provided by the invention can effectively promote polarization of M2-type macrophages to M1-type macrophages, shows remarkable immunoregulation ability, is good in biocompatibility, and has the potential of preparing anti-cancer drugs.

The invention discloses lactobacillus mucilaginosus and application thereof in fermentation of sporoderm-broken ganoderma lucidum spore powder. The preservation number of the lactobacillus mucilaginosus is CGMCC (China General Microbiological Culture Collection Center) No.31453. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age, is not hemolyzed, is sensitive to various common antibiotics and has strong lactic acid production capacity, and fermented supernate of the strain has strong growth inhibition capacity on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, shigella dysenteriae and gardnerella vaginalis and can be used for producing lactic acid. The compound has strong adhesion ability, growth inhibition ability and apoptosis promoting effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of bioactive substances such as amino acids and derivatives thereof, fatty acids and steroids in fermentation liquor can be greatly increased, the strain has an obvious anti-inflammatory effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in functional food and biological agents, but also opens up a new way for further development of ganoderma spores.

The invention relates to the field of biological agents, in particular to a compound probiotic preparation rich in organic selenium and a preparation method of the compound probiotic preparation, and efficient synergistic delivery is achieved through plant micromolecule organic selenium extraction and a multi-layer microemulsion embedding technology. The preparation consists of a core active component, a carrier system and a curcumin compound. The plant micromolecular organic selenium is extracted from cardamine hupingshanesis and high-selenium corn flour through an HLUP technology, and the bioavailability is remarkably improved. A corn protein modified W / O / W microemulsion is adopted as a carrier, an outer layer membrane is stable in an acid environment, and an inner layer accurately releases selenium to small intestines; a protective agent composed of trehalose and glucose inhibits ice crystal damage, and glutaraldehyde crosslinking enhances the mechanical strength of the membrane. Curcumin and selenium synergistically enhance antioxidant and immunomodulatory functions, and promote phagocytic activity of macrophages. The stability and bioavailability bottlenecks of a traditional preparation are broken through, and an innovative scheme is provided for functional food development.

The present disclosure relates to methods that enable the continuous formation of droplets and dehydration of droplets to provide pharmaceutically relevant particles that can be used for therapy. In particular, the methods disclosed herein allow the controlled continuous droplet formation and dehydration that produce circular particles having low internal void spaces comprising bioactive therapeutic biologics.

The invention relates to the technical field of biological agents, in particular to a goose astrovirus egg yolk antibody and a preparation method and application thereof. The invention provides a goose astrovirus egg yolk antibody. The goose astrovirus egg yolk antibody is prepared by adopting an inactivated vaccine as an antigen, wherein the inactivated vaccine is prepared from a goose astrovirus GAstV / SD / 36-95P strain with the preservation number of CCTCC (China Center For Type Culture Collection) NO: V202445. The neutralizing titer of the goose astrovirus egg yolk antibody provided by the invention is high and can reach 10.5 log2, after goslings are infected by the goose astrovirus, the goslings can be protected from disease attack and death by injecting the egg yolk antibody, and the protection rate of the egg yolk antibody can reach 100%.

The invention belongs to the technical field of biological control, and particularly relates to biological control fermentation liquor and a preparation method and application thereof, the preparation method comprises the following steps: S1, raw material treatment: selecting solidago canadensis as a plant raw material, cutting the solidago canadensis into pieces, performing autoclaved sterilization, adding water, and mixing to obtain a mixture; s2, adding the cultured bacillus subtilis and saccharomyces cerevisiae into the mixture in the step S1, mixing and inoculating, and stirring and fermenting to obtain a fermentation stock solution; s3, centrifuging the fermentation stock solution, taking supernate, and filtering to obtain the biological control fermentation liquor. The biological control fermentation liquor prepared by the invention can be used for controlling seed germination, seedling growth and flowering processes of solidago canadensis, and meanwhile, the biological control fermentation liquor can also be used for preparing products for controlling solidago canadensis. The problem that a biological medicament for preventing and treating solidago canadensis is lacked at present is solved.

The invention discloses a comprehensive prevention and control method for tobacco root knot nematode disease containing a biological agent. The method comprises the steps of crop rotation of tobacco and buckwheat / quinoa, host resistance induction of the microbial agent in the seedling raising period, tobacco planting soil quality improvement in the transplanting period and the like. The agronomic characters of tobacco seedlings are effectively improved, and the physical characters of soil are improved; the chemical properties of soil pH, alkali-hydrolyzable nitrogen, available phosphorus, quick-acting potassium, exchangeable calcium, exchangeable magnesium and the like are improved; the activity of soil urease, sucrase, acid phosphatase, catalase and peroxidase is improved; the content of water-soluble phenols, compound phenols and total phenols in soil is reduced; the disease index of the tobacco root knot nematode disease can be remarkably reduced, the survival rate of tobacco is increased, the application amount of chemical agents is reduced, and the healthy and sustainable development of the tobacco industry is promoted.

The invention provides a soil pollution intelligent monitoring and early warning method based on coupling of source accurate prevention and control and enhanced natural restoration, and relates to the technical field of pollution hidden danger investigation and source tracing. According to the method, toxic and harmful substances are identified through data collection, interview and reconnaissance, a key monitoring list is constructed, troubleshooting and leakage detection are carried out, hidden danger judgment and grading are completed, a rectification strategy is matched to generate a bioremediation scheme, biological agent injection or biological pile construction is carried out on a remediation area, and remediation effect evaluation is carried out. And setting monitoring factors and arranging monitoring point positions after the restoration reaches the standard, collecting monitoring data and inputting the monitoring data into a monitoring and early warning system, and realizing abnormal alarm through threshold triggering. According to the method, a closed-loop process from source identification, restoration implementation to long-term monitoring is constructed, and the accuracy and early warning efficiency of pollution treatment are improved.

The invention discloses a microbiological deodorization method, which belongs to the technical field of disinfection and deodorization, and comprises the following steps: S1, preparing a microbial preparation, S2, pre-stirring a strain combination; S3, preparing a deodorant from the prepared composite microbial preparation according to the mass; and S4, putting a prepared deodorant into an ultrasonic atomizer or a spray tower. According to the invention, the microbial preparation is creatively adopted to perform spray deodorization on the odor source, so that the deodorization efficiency is high, the effect is good, the treatment cost of the odor source and odor is effectively reduced, the overall operation difficulty is low, only the polluted area is atomized, the odor is effectively controlled before volatilization, the odor pollution spreading can be effectively controlled, and the influence on the operation environment is avoided; the microbial inoculum is non-toxic, can be naturally degraded, has no residue after deodorization, does not need additional neutralization and filtration, has higher environmental adaptability, and meets the deodorization requirements of small and medium-sized farms and small and medium-sized garbage collection stations.

The present disclosure provides for devices and systems for detecting biological agents (e.g., virus such as coronavirus) using nanotriangles having a capture agent. The present disclosure also includes methods of detecting the presence and amount of the biological agent in a sample, such as saliva, blood, or urine. In addition, the present disclosure provides for a detecting module and a method of making the detecting module, where the detecting module includes the nanotriangles having the capture agent bonded thereto that has an affinity for the biological agent.

The invention relates to genetically encoded fusion proteins comprised of a capture component that binds a target with high affinity and a peptide polymer, such as elastin-like polypeptides, that display phase behavior and can be used for purification. The invention further relates to methods for optimizing capture fusion proteins for individual biologic targets such that phase separation occurs under desirable conditions, such as at room temperature, lower concentrations of salt, and / or at suitable pH ranges and optimized capture domains and polypeptides with phase behavior that have been identified by the optimization methods.

The invention provides a biological agent with a scale inhibition effect. The biological agent comprises plant lactobacillus, pseudomonas and a saccharomyces cerevisiae strain. The biological scale inhibitor is a mixture of independent fermentation broth containing the three bacteria, the three bacteria are compounded according to different proportions, and the static scale inhibition effect is tested. The preparation is applied to 10L and 100L circulating water simulation systems, the microbial agent is added according to the proportion that the microbial agent is added every 7 days and the total amount of the microbial agent added each time accounts for 0.01%-0.05% of the circulating water, and compared with a control group, an experimental group added with the biological scale inhibitor shows better scale inhibition and algae inhibition effects within four weeks. The microbial agent disclosed by the invention is applied to a 2800m < 3 > circulating water cooling system for a scale inhibition test, and shows a stable scale inhibition effect compared with a conventional chemical scale inhibitor in a test period of 30 days.

The invention relates to the technical field of biological agents, and particularly discloses a preparation method of a freeze-dried varicella attenuated live vaccine and the freeze-dried varicella attenuated live vaccine. The preparation method of the freeze-dried varicella attenuated live vaccine provided by the invention comprises the following steps: culturing cells, infecting the cells with viruses, and harvesting the cells. Wherein the specific steps of harvesting the cells are as follows: adding a stabilizer into the washed cells, then harvesting a cell culture by using an ultrasonic cell harvesting device, and filtering the cell culture to obtain a stock solution; the process parameters in the ultrasonic cell harvesting device are as follows: the ultrasonic power is 60-90%, the ultrasonic time is 1.0-2.5 s, and the ultrasonic frequency is 1-2; the invention also provides the freeze-dried varicella attenuated live vaccine obtained by the preparation method. The preparation method of the freeze-dried varicella attenuated live vaccine provided by the invention has the advantages of simple process steps, short preparation period, high process stability and the like, and the obtained freeze-dried varicella attenuated live vaccine is good in quality consistency and good in clinical medication safety.

This invention discloses a system for predicting the efficacy of inflammatory bowel disease based on multimodal data, relating to the field of medical information processing. The system includes: a multimodal data acquisition module for receiving multimodal data; a text feature extraction module for determining text feature vectors using a text feature extraction model; an endoscopic intestinal mucosal feature recognition module for determining intestinal mucosal features using an endoscopic intestinal mucosal feature recognition model; an image feature recognition module for determining imaging features using an image feature recognition model; a pathological feature extraction module for determining pathological features using a pathological recognition model; and an efficacy prediction module for predicting the efficacy of biologics based on multimodal data features using a Transformer model and fully connected layers. Compared to existing technologies, this invention achieves efficacy prediction of biologics based on the fusion of clinical, pathological, endoscopic, and imaging multimodal features, improving the accuracy and clinical rationality of diagnostic and treatment decisions.

The invention provides an efficient composite sludge reduction biological agent which comprises bacillus coagulans, bacillus altitudinis, bacillus pumilus cellulase and efficient composite COD / NH3-N bacteria, the efficient composite COD / NH3-N bacteria comprise amylolytic bacteria, proteolytic bacteria, plant lactic acid bacteria and nitrifying bacteria, the preservation number of the bacillus coagulans is CGMCC NO.31765, and the preservation number of the bacillus altitudinis is CGMCC NO.31765. The preservation number of the bacillus altitudinis is CGMCC (China General Microbiological Culture Collection Center) NO.29011, and the preservation number of the bacillus pumilus is CGMCC NO.29013. The efficient composite sludge reduction biological agent is directly added into an aeration tank (aerobic tank) in a sludge treatment process, sludge (MLVSS) generated by a system is reduced in the digestion process of organic matters in a biochemical tank, the discharge of residual sludge is reduced, and the sludge age is prolonged.