376 results about "Biologic Agents" patented technology

The invention provides a soil pollution intelligent monitoring and early warning method based on coupling of source accurate prevention and control and enhanced natural restoration, and relates to the technical field of pollution hidden danger investigation and source tracing. According to the method, toxic and harmful substances are identified through data collection, interview and reconnaissance, a key monitoring list is constructed, troubleshooting and leakage detection are carried out, hidden danger judgment and grading are completed, a rectification strategy is matched to generate a bioremediation scheme, biological agent injection or biological pile construction is carried out on a remediation area, and remediation effect evaluation is carried out. And setting monitoring factors and arranging monitoring point positions after the restoration reaches the standard, collecting monitoring data and inputting the monitoring data into a monitoring and early warning system, and realizing abnormal alarm through threshold triggering. According to the method, a closed-loop process from source identification, restoration implementation to long-term monitoring is constructed, and the accuracy and early warning efficiency of pollution treatment are improved.

The invention discloses a microbiological deodorization method, which belongs to the technical field of disinfection and deodorization, and comprises the following steps: S1, preparing a microbial preparation, S2, pre-stirring a strain combination; S3, preparing a deodorant from the prepared composite microbial preparation according to the mass; and S4, putting a prepared deodorant into an ultrasonic atomizer or a spray tower. According to the invention, the microbial preparation is creatively adopted to perform spray deodorization on the odor source, so that the deodorization efficiency is high, the effect is good, the treatment cost of the odor source and odor is effectively reduced, the overall operation difficulty is low, only the polluted area is atomized, the odor is effectively controlled before volatilization, the odor pollution spreading can be effectively controlled, and the influence on the operation environment is avoided; the microbial inoculum is non-toxic, can be naturally degraded, has no residue after deodorization, does not need additional neutralization and filtration, has higher environmental adaptability, and meets the deodorization requirements of small and medium-sized farms and small and medium-sized garbage collection stations.

An “inverse” precipitation route to precipitate aqueous soluble species with copolymers as nanoparticles having a hydrophilic, polar core and a less polar shell is described.

The invention belongs to the technical field of biological medicines, and discloses application of vitamin K3 in preparation of a medicine for treating psoriasis. The invention finds that the compound vitamin K3 can obviously inhibit the activation of the STING downstream immune pathway and has an inhibition effect on the activation of the STING pathway. A mouse psoriasis model is formulated according to the absorption and metabolism characteristics of the inhibitor VK3, and it is proved that the VK3 can significantly inhibit the process of psoriasis and up-regulation of an STING pathway at an affected part in a transdermal drug delivery mode. The STING pathway does not belong to a main pathway affecting the course of disease in most non-immunodeficient inflammatory diseases, so that a better treatment effect may be generated by using the STING pathway in combination with a direct treatment drug (such as an IL-17A antibody for psoriasis). The vitamin K3 is simple in chemical structure, mature in production process and low in preparation cost, has more remarkable price advantage compared with biological agents and small-molecule targeting drugs, and has high popularization value and application prospects.

The invention discloses a bacillus velezensis YP-5 strain and an application thereof. Relates to the technical field of microorganisms. The invention provides a preservation number, a fungicide, a method for improving the planting capacity of bacillus velezensis in tea tree leaves and specific application. The bacillus velezensis YP-5 is high in control effect and wide in control spectrum. In addition, under the technological conditions of the invention, the quantity of colonized bacteria on tea tree leaves is increased by 1.9 times compared with that of a blank control group; after 7 days of treatment, the fixed planting bacteria quantity is 3.42 times of that of a blank control group. Meanwhile, when the microbial agent is sprayed to the tea trees in an indoor environment, the biological control effect on the tea anthracnose can be remarkably enhanced, and the biological control effect is improved by 10.29% compared with that of original YP-5 bacterial liquid. The problem that in the prior art, microbial thalli are weak in adhesion and prone to loss, and consequently the biocontrol capacity of the microbial thalli is weakened is effectively solved, and the method is suitable for industrial application of agricultural microbial preparations.

The invention relates to the technical field of microorganisms, and particularly discloses a lactobacillus paracasei KQNIU, a microbial preparation and application of the lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, the preservation number is CGMCC No.35739, and the Lactobacillus paracasei KQNIU is classified and named as Lactobacillus paracasei, and the Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The lactobacillus paracasei KQNIU provided by the invention has good gastrointestinal tract tolerance, has an inhibition effect on pathogenic bacteria such as escherichia coli, salmonella and the like, can be prepared into a microbial preparation or a feed additive for preventing and treating calf diarrhea, replaces antibiotics and realizes green and healthy breeding.

The invention relates to the technical field of biological medicines, and discloses a PD-L1 K263 acetylation modified antibody, which is prepared from an immunogen containing a sequence as shown in SEQ ID NO.1. The PD-L1 K263 acetylation modified antibody is a PD-L1 K263 acetylation modified antibody. The invention also discloses a preparation method of the PD-L1 K263 acetylation modified antibody. The preparation method comprises the following steps: designing and synthesizing a polypeptide antigen, preparing an immunogen, immunizing animals, purifying the antibody and evaluating serum titer. The antibody provided by the invention can accurately recognize and combine with the PD-L1 protein subjected to K263 acetylation modification in the breast cancer, provides a specific tool for qualitative and quantitative detection of K263Ac-PD-L1 in the breast cancer, and provides a reliable molecular marker detection means for evaluating the curative effect of anti-PD-1 / PD-L1 immunotherapy; therefore, a biological preparation with specificity and practicability and a technical support are provided for accurate diagnosis, targeted therapy and curative effect monitoring of breast cancer.

The application provides a bacillus desiccabilis strain XT23 microbial agent, a preparation method and application thereof, and belongs to the technical field of biological agents. A biological agent comprises a bacillus desiccabilis strain XT23, a fermentation treatment liquid of the bacillus desiccabilis strain XT23, or supernatant separated from the fermentation treatment liquid of the bacillus desiccabilis strain XT23. The fermentation treatment liquid is obtained by acid treatment and heat treatment of the fermentation liquid of the bacillus desiccabilis strain XT23, which effectively promotes spore formation, inactivates miscellaneous bacteria, significantly improves the stability and storage period of effective live bacteria, maximally retains antibacterial active substances in the fermentation liquid, and makes the inhibition effect of the final biological agent on Vibrio parahaemolyticus equivalent to that of fresh fermentation liquid. Meanwhile, the addition of the auxiliary material has a protective and slow-release effect on the active ingredients, and further enhances the convenience and effect persistence of application.

The application relates to the field of biological preparations, and particularly discloses an immunochromatographic reagent for detecting malignant tumors in urine and application thereof, which is used for solving the problems of poor recognition of tumor specific epitopes, heavy background interference, spectrum overlap and high subjectivity of artificial judgment in the single staining and color development of acridine orange in the detection of urinary exfoliative cells of bladder cancer. The color development reagent comprises A reagent, B reagent, C reagent and D reagent. The A reagent is provided with an antibody complex for target antigens of bladder tumor cells in urine exfoliation and acridine orange. The B reagent comprises a fluorescently labeled secondary antibody composition corresponding to the A reagent and an enzyme-labeled secondary antibody-tyramide deposition amplification composition. In the application, AI imaging analysis is adopted to calibrate layered recognition labels and acridine orange staining negative and positive labels. Through multi-target immunochromatography, two sets of color separation amplification schemes and AI imaging analysis, the application improves the recognition ability of bladder tumor specific epitopes, the image quality and the image analysis accuracy in the detection of urinary exfoliative cells.

The invention discloses a double-cavity clamping type bottle injection pen which is characterized in that a lower shell wraps the outer part of a double-cavity injection tube, and the upper part of the double-cavity injection tube is communicated with an injection push rod in the vertical direction; a first piston and a second piston are arranged in an inner cavity of the double-cavity injection tube, so that a first cavity is formed by the first piston and the bottom of the double-cavity injection tube, and a powder medicament or a liquid medicament A is contained in the first cavity; a closed second cavity is formed in the inner cavity of the double-cavity injection tube between the first piston and the second piston, and a liquid medicament B is contained in the second cavity; a communication bridge protruding outwards is arranged on the inner wall of the double-cavity injection tube, the first cavity and the second cavity can be communicated through the communication bridge by pushing the first piston and the second piston to move, and mixing of the medicine liquid B and the powder medicine or the liquid medicine A is achieved. Through the modular design, the double-cavity medicament injection function which is compact in structure and easy and convenient to operate is achieved, and the device is particularly suitable for biological agent or vaccine injection scenes needing on-site mixing.

This invention discloses an in-situ three-dimensional bioremediation method for slightly polluted water bodies. The method involves collecting a mud-water mixture from the water body to be treated, enriching and culturing it, and collecting the settled sludge. Culture medium II is added to the settled sludge for acclimatization and cultivation to prepare a biological agent. This biological agent is then added to a mixed solution of polyvinyl alcohol-hydrated calcium silicate-fulvic acid (PVA-CSH-FA) or PVA-modified zeolite-FA to prepare an immobilized biological carrier that can slowly release or adsorb calcium ions from the water. Calcium peroxide packing is also prepared to provide oxygen. The immobilized biological carrier packing and calcium peroxide packing are respectively fixed in the upper and lower layers of an ecological sedimentation bed, which is then placed in the slightly polluted water body to achieve in-situ three-dimensional bioremediation. The calcium-precipitating bacterial biological agent obtained through enrichment and acclimatization in this invention, when immobilized and mixed with the carrier material, efficiently and simultaneously removes pollutants such as nitrogen, phosphorus, organic matter, and heavy metals from slightly polluted water bodies.

The present application relates to a strain of Streptomyces griseoaurantiacus and its application, belonging to the technical field of biological control of plant diseases. The Streptomyces griseoaurantiacus of the present application has been preserved in Guangdong Microbial Culture Collection Center on July 28, 2023, with the preservation number of GDMCC No: 63691 and the preservation address of No. 59 Building, 5th Floor, 100, Martyrs' Road, Guangzhou, Guangdong Province. The strain can inhibit the formation of Ralstonia solanacearum biofilm and the occurrence of Ralstonia solanacearum caused by bacterial wilt, has good prevention and control effect on bacterial wilt, and can be used as a biocontrol agent and biological preparation for the prevention and control of bacterial wilt.

This invention discloses a strain of *Bifidobacterium pseudosporidis*, its preparation method, and its application in the prevention and treatment of METH addiction. *Bifidobacterium pseudosporidis* was isolated from fecal samples of individuals with low levels of METH addiction, and its strain identity was confirmed after isolation, purification, and identification. A METH addiction model was constructed by gavage colonization of C57 mice with this strain, and addiction-related indicators were detected in the model mice. The results showed that compared with the addiction model mice without colonization of this strain, the conditioned place preference score of the colonized group was significantly reduced (P<0.05). This *Bifidobacterium pseudosporidis* has a significant effect on improving METH addictive behavior and can be used to prepare biological agents with preventive and / or therapeutic functions for METH addiction. This invention reveals that *Bifidobacterium pseudosporidis* has the advantages of high safety and strong targeting, and can achieve the prevention and intervention of METH addiction without complex treatment equipment, providing a novel biological treatment option for the clinical treatment of METH addiction.

The invention discloses a novel antifreeze polypeptide with a clear structure and excellent performance and a protection method of cryopreserved cells. The core structure of the polypeptide is formed by covalently connecting two functional modules, the N end is a unit with ice crystal binding performance, the C end is a super-hydrophilic unit, and the two functional modules are bridged through a flexible linker. Experimental verification shows that the synthetic polypeptide shows excellent anti-freezing activity, can significantly inhibit nucleation, growth and recrystallization processes of ice crystals, and has particularly outstanding ice recrystallization inhibition activity. The novel antifreeze polypeptide shows excellent biocompatibility, and is suitable for low-temperature cryopreservation of cells sensitive to freezing damage and other living microbial preparations. Results show that the polypeptide can greatly improve the freeze-drying survival rate of cells and the activity of the cells after recovery, is non-toxic to the cells, and shows the potential of replacing a traditional cryoprotectant. Therefore, the method has wide application prospects in food industry, biological medicine, agricultural breeding and low-temperature preservation technologies of biological sample libraries.

The application discloses a specific nucleic acid aptamer for a heterogeneous core ribonucleoprotein hnRNPA2B1 and application, and belongs to the technical field of nucleic acid aptamer. The hnRNPA2B1 aptamer is a small single-stranded DNA screened from a designed oligonucleotide library through an in-vitro exponential enrichment ligand system evolution (SELEX), is composed of 80 nucleotides, and has high affinity to the target. The hnRNPA2B1 aptamer can be applied in a detection method or a detection product of the hnRNPA2B1 protein, and a targeted regulation drug or a biological preparation, and can be specifically made into a molecular probe, used as a detection reagent, used as a targeted preparation and the like for diagnosis and treatment of diseases related to the hnRNPA2B1 protein. The hnRNPA2B1 aptamer has strong affinity and specificity, has the advantages of low production cost, stability, convenient synthesis and easy modification compared with an antibody, and is a potential substitute for the antibody in disease diagnosis and treatment.

The invention provides application of an FZD4 gene. The FZD4 gene is used for regulating osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells. The FZD4 gene in the chicken bone marrow mesenchymal stem cells is over-expressed and is used for promoting osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; or / and the FZD4 gene in the chicken bone marrow mesenchymal stem cells is knocked down to inhibit osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; the nucleotide sequence of the coding region of the FZD4 gene is as shown in SEQ ID NO. 1. According to the invention, the positive regulation effect of the FZD4 gene in osteogenic differentiation of chicken bone marrow mesenchymal stem cells is determined for the first time, the blank of a bone regulation mechanism of the poultry FZD4 gene is filled, and a new biological preparation target is provided for prevention and treatment of poultry bone diseases; the FZD4 gene can also be used as a molecular marker for breeding chicken varieties with high bone strength.

This invention provides a biological compound preparation for repelling insects or reducing the oviposition of flying insects, its preparation method, and its application. The biological compound preparation for repelling insects or reducing the oviposition of flying insects includes a chemical reagent and a biological agent; wherein the chemical reagent includes 2-phenylethanol, and the biological agent includes *Bacillus amyloliquefaciens* and *Bacillus brevis*; the preservation number of *Bacillus amyloliquefaciens* is CGMCC No. 35162, and the preservation number of *Bacillus brevis* is CGMCC No. 35161. The core advantage of this invention lies in the synergistic effect of 2-phenylethanol (chemical reagent) with *Bacillus amyloliquefaciens* (CGMCC No. 35162) and *Bacillus brevis* (CGMCC No. 35161) (biological agent). Through complementary and mutually reinforcing mechanisms, the two significantly enhance the control effect.

The invention discloses a potato late blight antagonistic bacterium and application thereof, belongs to biological agents, and provides more strain resources for prevention and treatment of potato late blight. The potato late blight antagonistic bacterium is Pseudomonas chlororaphis blT-49 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 27, 2021, and the preservation number is CGMCC No.24203. The invention further discloses a preparation method of the potato late blight antagonistic bacterium. The strain disclosed by the invention not only can inhibit the occurrence and growth of potato late blight, but also can regulate and control the ecological system of the micro-ecology of potatoes, reduce the infection ability of potato late blight and improve the disease resistance of potatoes. In the growth and development of the potatoes, the growth and development of the potatoes can be promoted, and the single-plant yield is improved.

The invention relates to a biological agent, in particular to a preparation method of the biological agent, which comprises the following steps: S1, injecting a cultured biological bacterium culture solution into a centrifugal space formed by a plurality of separation rings and a plurality of separation cavities; s2, a plurality of separation rings and a plurality of separation cavities are driven to rotate, and a plurality of water spraying holes spray liquid from top to top to impact the biological bacteria; s3, the biological bacteria with large mass are left on the lower side, and the biological bacteria with small mass are flushed to the upper side by the liquid; s4, after the biological bacteria are separated according to the mass, a plurality of separation rings rotate to discharge the liquid; s5, the biological bacteria are left in the centrifugal space; s6, pressure gas is introduced into a plurality of separation cavities, and an elastic inclined plate is inclined and expands to separate the biological bacteria according to the mass; and the biological bacteria can be centrifugally classified according to different masses.

The present disclosure provides materials and methods related to the purification of a biologic from a biological fluid. In particular, the present disclosure provides compositions, and related methods, comprising peptide ligands capable of removing process-related impurities (e.g., host cell proteins, nucleic acids, and media components) and product-related impurities (e.g., product fragments, product aggregates, and inactive forms derived from product degradation by or association with other species in the cell culture harvest) from biological fluids during the production of a biologic.

This invention relates to an anti-swine pseudorabies virus (PRV) preparation and the application of Rehmannia glutinosa polysaccharide in its preparation, belonging to the field of biopharmaceutical technology. This invention provides an anti-swine pseudorabies virus (PRV) preparation comprising Rehmannia glutinosa polysaccharide. It also provides the application of Rehmannia glutinosa polysaccharide in the preparation of an anti-PRV preparation for the prevention and / or treatment of swine pseudorabies. This invention utilizes Rehmannia glutinosa polysaccharide to prevent infection by the PRV variant XJ5 by reducing intracellular oxidative stress levels; it also resists viral infection by affecting viral adsorption. Furthermore, when the concentration of Rehmannia glutinosa polysaccharide is between 50 and 400 μg / mL, it can also affect viral entry and replication, and has no cytotoxicity to PK-15 cells.

The utility model provides a pore plate filling device, and belongs to the technical field of biological preparation production, the device comprises: a base on which a placing table is arranged for placing a pore plate parallel to the base, the placing table is movably arranged along the length direction of the base; the mounting table is arranged on the base, located at one end of the placing table, used for placing the liquid storage disc parallel to the base and movably arranged in the height direction of the base; the mounting plate is erected above the mounting table and parallel to the base, the mounting plate is movably arranged in the height direction of the base, and a stirring assembly used for stirring the mixed liquid in the liquid storage disc is arranged on the mounting plate; and the mounting frame is erected above the placement table and is movably arranged in the length direction of the base, and a pipetting assembly is arranged in the mounting frame and is used for sucking the mixed liquid in the liquid storage disc and filling the mixed liquid into the pore plate. The filling device has high automation degree, and can effectively solve the problem of poor filling uniformity.

This invention belongs to the field of biological agents for aquaculture, and discloses a compound biological pond-cleaning agent containing tea saponin and its preparation method. The compound biological pond-cleaning agent of this invention comprises the following components by weight: 3-10 parts tea saponin, 60-70 parts roe beetle root powder, 10-20 parts Buddleja officinalis, 5-10 parts compound microbial powder, 3-8 parts adjuvants, and 10-20 parts carrier; the adjuvant is a mixture of sodium lignin sulfonate and sodium dodecylbenzene sulfonate; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria; and the carrier is diatomaceous earth. This invention combines three natural active ingredients—tea saponin, roe beetle root powder, and Buddleja officinalis—with suitable adjuvants and a carrier, which can expand the pond-cleaning range and has good killing or inhibitory effects on harmful organisms such as wild fish, shrimp, crabs, and snails, as well as various pathogens. The pond-cleaning is thorough, and the raw materials are easily degradable, leaving no residue. It is environmentally friendly and has a long-lasting effect, which is of great significance for promoting the green and sustainable development of aquaculture.

The invention provides an application of 10E, 12Z-octadecadienoic acid in inhibition of inflammatory reactions, and the inflammatory reactions comprise macrophage inflammatory reactions induced by lipopolysaccharide. According to the invention, a mouse mononuclear macrophage RAW264.7 cell inflammatory response model is constructed. The method comprises the following steps: firstly, measuring CC50 of 10E, 12Z-octadecadienoic acid by a CCK-8 method; 10E, 12Z-octadecadienoic acid is used for pretreating macrophages for 24 hours before lipopolysaccharide stimulation at the final concentration of 25 mu mol / L to 100 mu mol / L, qPCR (quantitative polymerase chain reaction) and ELISA (enzyme-linked immuno sorbent assay) experiments prove that the 10E, 12Z-octadecadienoic acid plays an anti-inflammatory role by inhibiting mRNA (messenger ribonucleic acid) expression and protein secretion of IL-6, IL-1beta and / or TNF-alpha, and the anti-inflammatory effect is optimal at the final concentration of 100 mu mol / L. The 10E, 12Z-octadecadienoic acid is a single unmodified fatty acid isomer, is clear in structure, good in anti-inflammatory effect repeatability and good in biocompatibility, and can reduce adverse reactions compared with traditional anti-inflammatory drugs; compared with existing fatty acid anti-inflammatory components, the composition is clear in component and simple and convenient to prepare. The application scenarios of the invention cover the development of anti-inflammatory drugs, functional foods and biological agents.

The invention relates to the technical field of biological preparations, and discloses a periodontitis improving compound preparation containing fermented lactobacillus mucus and traditional Chinese medicine extracts, and the periodontitis improving compound preparation comprises the following raw materials: an oral preparation: fermented lactobacillus mucus CQPC202403 bacterial powder; the gel comprises scutellaria baicalensis, honeysuckle, salvia miltiorrhiza, liquorice and a hydrogel matrix; the preservation number of the lactobacillus mucus CQPC202403 in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC NO.31106, and the preservation date of the lactobacillus mucus CQPC202403 is June 27, 2024. The lactobacillus mucilaginosus adopted by the invention inhibits pathogenic bacteria through competitive rejection and immunoregulation; the traditional Chinese medicine extracts such as the radix scutellariae directly destroy bacterial pellicles, and the radix scutellariae and the traditional Chinese medicine extracts cooperate with each other, so that the antibacterial effect is enhanced on the premise of not causing drug resistance.

The invention provides an enterococcus faecium probiotic-prebiotics composite tablet and a preparation method thereof, and belongs to the technical field of microbial preparations. The tablet is prepared by taking enterococcus faecium (preservation number: CGMCC NO.33919) as a core active component, matching prebiotics adapted to the intestinal tracts of old people, a high-stability protective agent and mild auxiliary materials through a specific process. The tablet can efficiently adjust the intestinal flora structure of the old people, inhibit the proliferation of harmful bacteria in the intestinal tract and enhance the intestinal barrier function of the old people, so that the functional diarrhea and antibiotic-related diarrhea of the old people are relieved, and the tablet is moderate in hardness, easy to swallow, free of intestinal irritation and high in normal-temperature storage stability; the problems that an existing intestinal preparation is poor in adaptability to intestinal tracts of old people, strain activity is prone to inactivation, and swallowing is difficult are solved.

The invention relates to a preparation of an antibody drug, which belongs to the field of biological medicines, is used for a preparation of a high-concentration antibody drug with an anti-oxidation requirement, and also has an anti-degradation capability. The preparation contains 20 mmol / L to 30 mmol / L of L-methionine and 200 mmol / L of trehalose, and is particularly suitable for being used for preparing Jukoulikumab, omaszumab and cetuximab. The preparation not only has a better anti-oxidation effect, but also can realize a synergistic stabilization effect by combining high-concentration L-Met with 200 mM trehalose, has anti-degradation ability, is widely applicable to various therapeutic antibodies, and provides an extensible solution for improving the stability and shelf life of a high-concentration biological preparation.

The invention relates to the technical field of biology, and aims to provide an M13 recombinant bacteriophage with PRRSV broad-spectrum neutralizing activity for displaying NPC and application of the M13 recombinant bacteriophage. A nano antibody-CD163 peptide fragment conjugate NPC shown as SEQ ID NO: 1 is displayed on the surface of the N end of capsid protein III of the M13 recombinant phage, 1-3 NPC molecules are contained on the surface of each phage particle, and the phage is used for neutralizing PRRSV (Porcine Reproductive and Respiratory Syndrome Virus). The recombinant bacteriophage provided by the invention is easy to prepare, extremely high in virus titer and excellent in thermal and pH stability; in Marc-145 and PAMs cells, the compound shows strong neutralizing activity on PRRSV II type 1, 3, 5 and 8 pedigree, so that the compound has broad-spectrum neutralizing capacity; the compound can be used as a PRRSV prevention and control preparation, and a new thought is provided for development of safe and efficient novel antiviral drugs or biological preparations.

This invention belongs to the field of organic agriculture, specifically a biological agent for promoting wheat photosynthesis and its preparation method. Addressing the problems of existing biological agents having relatively simple components, low absorption and utilization efficiency by wheat ears, and a tendency to reduce nitrate reductase activity, nitrogen utilization, and chlorophyll content in wheat leaves, the following solution is proposed: the biological agent comprises the following raw materials in parts by weight: 50-65 parts of yield-enhancing amine, 15-20 parts of choline chloride, 10-15 parts of diethylaminoethanol hexanoate, 5-10 parts of brassinolide, 3-5 parts of p-chlorophenoxyacetic acid, 1-3 parts of calcium diphenylureasulfonate, 2-5 parts of trace agents, and 2-4 parts of ethylene glycol styraldehyde. The biological agent of this invention can promote better photosynthesis in wheat, enhance nitrate reductase activity, and improve nitrogen utilization.

The application discloses a polypeptide GRN-E and application thereof in preparation of a medicine or preparation for perimenopausal emotional disorder. It is found for the first time that a GRN-E fragment (a C-terminal domain of PGRN, an amino acid sequence is SEQ ID NO. 1 in a sequence listing) can pass through a blood-brain barrier and has a unique effect in relieving perimenopausal anxiety and depression. As a key functional fragment, the GRN-E has a more concentrated effect on a central nervous system and can effectively avoid off-target effects of a full-length protein. The polypeptide GRN-E of the application can be applied to preparation of a medicine or preparation for perimenopausal emotional disorder as an innovative treatment target and a novel biological preparation and has a high clinical conversion value.