587 results about "Biologic Agents" patented technology

The invention provides a soil pollution intelligent monitoring and early warning method based on coupling of source accurate prevention and control and enhanced natural restoration, and relates to the technical field of pollution hidden danger investigation and source tracing. According to the method, toxic and harmful substances are identified through data collection, interview and reconnaissance, a key monitoring list is constructed, troubleshooting and leakage detection are carried out, hidden danger judgment and grading are completed, a rectification strategy is matched to generate a bioremediation scheme, biological agent injection or biological pile construction is carried out on a remediation area, and remediation effect evaluation is carried out. And setting monitoring factors and arranging monitoring point positions after the restoration reaches the standard, collecting monitoring data and inputting the monitoring data into a monitoring and early warning system, and realizing abnormal alarm through threshold triggering. According to the method, a closed-loop process from source identification, restoration implementation to long-term monitoring is constructed, and the accuracy and early warning efficiency of pollution treatment are improved.

The invention discloses a microbiological deodorization method, which belongs to the technical field of disinfection and deodorization, and comprises the following steps: S1, preparing a microbial preparation, S2, pre-stirring a strain combination; S3, preparing a deodorant from the prepared composite microbial preparation according to the mass; and S4, putting a prepared deodorant into an ultrasonic atomizer or a spray tower. According to the invention, the microbial preparation is creatively adopted to perform spray deodorization on the odor source, so that the deodorization efficiency is high, the effect is good, the treatment cost of the odor source and odor is effectively reduced, the overall operation difficulty is low, only the polluted area is atomized, the odor is effectively controlled before volatilization, the odor pollution spreading can be effectively controlled, and the influence on the operation environment is avoided; the microbial inoculum is non-toxic, can be naturally degraded, has no residue after deodorization, does not need additional neutralization and filtration, has higher environmental adaptability, and meets the deodorization requirements of small and medium-sized farms and small and medium-sized garbage collection stations.

This invention discloses a system for predicting the efficacy of inflammatory bowel disease based on multimodal data, relating to the field of medical information processing. The system includes: a multimodal data acquisition module for receiving multimodal data; a text feature extraction module for determining text feature vectors using a text feature extraction model; an endoscopic intestinal mucosal feature recognition module for determining intestinal mucosal features using an endoscopic intestinal mucosal feature recognition model; an image feature recognition module for determining imaging features using an image feature recognition model; a pathological feature extraction module for determining pathological features using a pathological recognition model; and an efficacy prediction module for predicting the efficacy of biologics based on multimodal data features using a Transformer model and fully connected layers. Compared to existing technologies, this invention achieves efficacy prediction of biologics based on the fusion of clinical, pathological, endoscopic, and imaging multimodal features, improving the accuracy and clinical rationality of diagnostic and treatment decisions.

The invention relates to the technical field of biological agents, and discloses a passivating enzyme soluble concentrate and a preparation process thereof.The soluble concentrate takes a specially-designed multifunctional deep eutectic solvent as a unique carrier, and the solvent has the multiple functions of stabilizing enzyme conformation, resisting oxidation, providing plant metabolism and increasing energy and the like. An active component is composite passivating enzyme powder which is subjected to vacuum freeze drying, and is compounded with a plant-derived phloem conduction enhancer in a synergetic manner. The preparation process adopts a core strategy of curing first and then dissolving, and the enzyme freeze-dried powder is dispersed in the non-aqueous solvent under low-temperature mild conditions. By constructing a water-free environment and adopting an activity protection process, the problems that a traditional water-based enzyme preparation is unstable in storage and the activity is easy to lose are fundamentally solved, and meanwhile, the bioavailability is remarkably improved by virtue of a targeted conduction component. The preparation has the remarkable advantages of ultra-long stability at normal temperature, high initial enzyme activity and strong targeting property.

An “inverse” precipitation route to precipitate aqueous soluble species with copolymers as nanoparticles having a hydrophilic, polar core and a less polar shell is described.

The invention belongs to the technical field of biological medicines, and discloses application of vitamin K3 in preparation of a medicine for treating psoriasis. The invention finds that the compound vitamin K3 can obviously inhibit the activation of the STING downstream immune pathway and has an inhibition effect on the activation of the STING pathway. A mouse psoriasis model is formulated according to the absorption and metabolism characteristics of the inhibitor VK3, and it is proved that the VK3 can significantly inhibit the process of psoriasis and up-regulation of an STING pathway at an affected part in a transdermal drug delivery mode. The STING pathway does not belong to a main pathway affecting the course of disease in most non-immunodeficient inflammatory diseases, so that a better treatment effect may be generated by using the STING pathway in combination with a direct treatment drug (such as an IL-17A antibody for psoriasis). The vitamin K3 is simple in chemical structure, mature in production process and low in preparation cost, has more remarkable price advantage compared with biological agents and small-molecule targeting drugs, and has high popularization value and application prospects.

The invention discloses a bacillus velezensis YP-5 strain and an application thereof. Relates to the technical field of microorganisms. The invention provides a preservation number, a fungicide, a method for improving the planting capacity of bacillus velezensis in tea tree leaves and specific application. The bacillus velezensis YP-5 is high in control effect and wide in control spectrum. In addition, under the technological conditions of the invention, the quantity of colonized bacteria on tea tree leaves is increased by 1.9 times compared with that of a blank control group; after 7 days of treatment, the fixed planting bacteria quantity is 3.42 times of that of a blank control group. Meanwhile, when the microbial agent is sprayed to the tea trees in an indoor environment, the biological control effect on the tea anthracnose can be remarkably enhanced, and the biological control effect is improved by 10.29% compared with that of original YP-5 bacterial liquid. The problem that in the prior art, microbial thalli are weak in adhesion and prone to loss, and consequently the biocontrol capacity of the microbial thalli is weakened is effectively solved, and the method is suitable for industrial application of agricultural microbial preparations.

The invention relates to the technical field of microorganisms, and particularly discloses a lactobacillus paracasei KQNIU, a microbial preparation and application of the lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, the preservation number is CGMCC No.35739, and the Lactobacillus paracasei KQNIU is classified and named as Lactobacillus paracasei, and the Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The lactobacillus paracasei KQNIU provided by the invention has good gastrointestinal tract tolerance, has an inhibition effect on pathogenic bacteria such as escherichia coli, salmonella and the like, can be prepared into a microbial preparation or a feed additive for preventing and treating calf diarrhea, replaces antibiotics and realizes green and healthy breeding.

A method and kit are provided for detecting anti-drug antibodies (ADAs) against self-assembling trimeric biologics, such as XPro1595, a pegylated variant of soluble human Tumor Necrosis Factor, in biological samples, particularly human serum. The method employs an Affinity Capture Elution-Protein A / G (ACE-AG) assay that overcomes non-specific reagent interactions inherent in conventional bridging and standard ACE assays due to the biologic's dynamic monomer exchange. The assay utilizes a two-plate system: a streptavidin-coated plate with biotinylated trimeric biologic captures ADAs, which are eluted, neutralized, and transferred to a protein A / G-coated plate for specific immunoglobulin capture and detection with a sulfo-tagged trimeric biologic via electrochemiluminescence. The kit includes pre-coated plates, labeled biologics, elution and neutralization solutions, assay buffer, confirmatory reagent, and instructions. The assay offers a robust, specific, and sensitive solution for immunogenicity testing of trimeric biologics.

A peptidoglycan hydrolase LysPH1 having a broad-spectrum lytic activity, and a mutant and the use thereof, which belong to the technical field of biological formulations. An antibacterial peptide LysPH1-CP, a peptidoglycan hydrolase LysPH1 containing the antibacterial peptide LysPH1-CP and a peptidoglycan hydrolase mutant LysPH1-K containing the peptidoglycan hydrolase LysPH1 all have good thermal stability, and can broadly lyse both Gram-negative bacteria and Gram-positive bacteria. Moreover, the peptidoglycan hydrolase LysPH1 and the peptidoglycan hydrolase mutant LysPH1-K can be solubly expressed in Escherichia coli, and have high enzymatic activity. Therefore, the antibacterial peptide LysPH1-CP, the peptidoglycan hydrolase LysPH1 and the peptidoglycan hydrolase mutant LysPH1-K have good application prospects in the preparation of an anti-infective drug and the research and development of feed additives.

The invention relates to the technical field of biological agents, and particularly discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent provided by the invention comprises compound bacillus, a carrier and a coating agent, the composite bacillus comprises bacillus velezensis, bacillus atrophaeus and bacillus firmus with the viable count ratio of (3-7): (1-4): 2; the viable count of the compound microbial agent is greater than or equal to 5 * 10 < 9 > cfu / g; the invention also provides a preparation method of the compound microbial agent. The preparation method comprises the following steps: strain fermentation, carrier adsorption, granulation and enveloping and low-temperature drying. The compound microbial agent provided by the invention can improve the soil structure, enhance the soil fertility and inhibit soil-borne diseases, so that the growth condition of crops is improved, and the quality and yield of the crops are improved.

The invention relates to the technical field of biological medicines, and discloses a PD-L1 K263 acetylation modified antibody, which is prepared from an immunogen containing a sequence as shown in SEQ ID NO.1. The PD-L1 K263 acetylation modified antibody is a PD-L1 K263 acetylation modified antibody. The invention also discloses a preparation method of the PD-L1 K263 acetylation modified antibody. The preparation method comprises the following steps: designing and synthesizing a polypeptide antigen, preparing an immunogen, immunizing animals, purifying the antibody and evaluating serum titer. The antibody provided by the invention can accurately recognize and combine with the PD-L1 protein subjected to K263 acetylation modification in the breast cancer, provides a specific tool for qualitative and quantitative detection of K263Ac-PD-L1 in the breast cancer, and provides a reliable molecular marker detection means for evaluating the curative effect of anti-PD-1 / PD-L1 immunotherapy; therefore, a biological preparation with specificity and practicability and a technical support are provided for accurate diagnosis, targeted therapy and curative effect monitoring of breast cancer.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

The invention relates to selenium-enriched pediococcus acidilactici Se34 with anti-oxidation, cholesterol-lowering and anti-tumor effects and application of the selenium-enriched pediococcus acidilactici Se34. The pediococcus acidilactici Se34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the pediococcus acidilactici Se34 is CGMCC No.33933, and the preservation date of the pediococcus acidilactici Se34 is March 24, 2025. The pediococcus acidilactici Se34 provided by the invention integrates excellent probiotic characteristics (acid-resistant bile salt, safe and colonizable), efficient selenium enrichment capacity and remarkably enhanced activity of resisting oxidation, reducing cholesterol and inhibiting specific cancer cell proliferation and metastasis after selenium enrichment. The strain and the selenium-rich product thereof have important application value in the fields of developing functional foods (selenium-rich probiotic products), antioxidant / cholesterol-reducing health-care foods, potential auxiliary anticancer biological preparations or prodrugs and the like.

The present invention relates to a polypeptide comprising the crystallizable fragment (Fc) region of IgG for use in the prevention or treatment of diseases, particularly diseases affecting the central nervous system. The polypeptide is administered locally to the affected compartment, particularly the central nervous system. The Fc region has a modification that reduces affinity for the neonatal Fc receptor (FcRn), resulting in increased brain to serum concentrations of the polypeptide.

The application provides a bacillus desiccabilis strain XT23 microbial agent, a preparation method and application thereof, and belongs to the technical field of biological agents. A biological agent comprises a bacillus desiccabilis strain XT23, a fermentation treatment liquid of the bacillus desiccabilis strain XT23, or supernatant separated from the fermentation treatment liquid of the bacillus desiccabilis strain XT23. The fermentation treatment liquid is obtained by acid treatment and heat treatment of the fermentation liquid of the bacillus desiccabilis strain XT23, which effectively promotes spore formation, inactivates miscellaneous bacteria, significantly improves the stability and storage period of effective live bacteria, maximally retains antibacterial active substances in the fermentation liquid, and makes the inhibition effect of the final biological agent on Vibrio parahaemolyticus equivalent to that of fresh fermentation liquid. Meanwhile, the addition of the auxiliary material has a protective and slow-release effect on the active ingredients, and further enhances the convenience and effect persistence of application.

The application relates to the field of biological preparations, and particularly discloses an immunochromatographic reagent for detecting malignant tumors in urine and application thereof, which is used for solving the problems of poor recognition of tumor specific epitopes, heavy background interference, spectrum overlap and high subjectivity of artificial judgment in the single staining and color development of acridine orange in the detection of urinary exfoliative cells of bladder cancer. The color development reagent comprises A reagent, B reagent, C reagent and D reagent. The A reagent is provided with an antibody complex for target antigens of bladder tumor cells in urine exfoliation and acridine orange. The B reagent comprises a fluorescently labeled secondary antibody composition corresponding to the A reagent and an enzyme-labeled secondary antibody-tyramide deposition amplification composition. In the application, AI imaging analysis is adopted to calibrate layered recognition labels and acridine orange staining negative and positive labels. Through multi-target immunochromatography, two sets of color separation amplification schemes and AI imaging analysis, the application improves the recognition ability of bladder tumor specific epitopes, the image quality and the image analysis accuracy in the detection of urinary exfoliative cells.

The invention discloses a double-cavity clamping type bottle injection pen which is characterized in that a lower shell wraps the outer part of a double-cavity injection tube, and the upper part of the double-cavity injection tube is communicated with an injection push rod in the vertical direction; a first piston and a second piston are arranged in an inner cavity of the double-cavity injection tube, so that a first cavity is formed by the first piston and the bottom of the double-cavity injection tube, and a powder medicament or a liquid medicament A is contained in the first cavity; a closed second cavity is formed in the inner cavity of the double-cavity injection tube between the first piston and the second piston, and a liquid medicament B is contained in the second cavity; a communication bridge protruding outwards is arranged on the inner wall of the double-cavity injection tube, the first cavity and the second cavity can be communicated through the communication bridge by pushing the first piston and the second piston to move, and mixing of the medicine liquid B and the powder medicine or the liquid medicine A is achieved. Through the modular design, the double-cavity medicament injection function which is compact in structure and easy and convenient to operate is achieved, and the device is particularly suitable for biological agent or vaccine injection scenes needing on-site mixing.

This invention discloses an in-situ three-dimensional bioremediation method for slightly polluted water bodies. The method involves collecting a mud-water mixture from the water body to be treated, enriching and culturing it, and collecting the settled sludge. Culture medium II is added to the settled sludge for acclimatization and cultivation to prepare a biological agent. This biological agent is then added to a mixed solution of polyvinyl alcohol-hydrated calcium silicate-fulvic acid (PVA-CSH-FA) or PVA-modified zeolite-FA to prepare an immobilized biological carrier that can slowly release or adsorb calcium ions from the water. Calcium peroxide packing is also prepared to provide oxygen. The immobilized biological carrier packing and calcium peroxide packing are respectively fixed in the upper and lower layers of an ecological sedimentation bed, which is then placed in the slightly polluted water body to achieve in-situ three-dimensional bioremediation. The calcium-precipitating bacterial biological agent obtained through enrichment and acclimatization in this invention, when immobilized and mixed with the carrier material, efficiently and simultaneously removes pollutants such as nitrogen, phosphorus, organic matter, and heavy metals from slightly polluted water bodies.

The present application relates to a strain of Streptomyces griseoaurantiacus and its application, belonging to the technical field of biological control of plant diseases. The Streptomyces griseoaurantiacus of the present application has been preserved in Guangdong Microbial Culture Collection Center on July 28, 2023, with the preservation number of GDMCC No: 63691 and the preservation address of No. 59 Building, 5th Floor, 100, Martyrs' Road, Guangzhou, Guangdong Province. The strain can inhibit the formation of Ralstonia solanacearum biofilm and the occurrence of Ralstonia solanacearum caused by bacterial wilt, has good prevention and control effect on bacterial wilt, and can be used as a biocontrol agent and biological preparation for the prevention and control of bacterial wilt.

This invention discloses a strain of *Bifidobacterium pseudosporidis*, its preparation method, and its application in the prevention and treatment of METH addiction. *Bifidobacterium pseudosporidis* was isolated from fecal samples of individuals with low levels of METH addiction, and its strain identity was confirmed after isolation, purification, and identification. A METH addiction model was constructed by gavage colonization of C57 mice with this strain, and addiction-related indicators were detected in the model mice. The results showed that compared with the addiction model mice without colonization of this strain, the conditioned place preference score of the colonized group was significantly reduced (P<0.05). This *Bifidobacterium pseudosporidis* has a significant effect on improving METH addictive behavior and can be used to prepare biological agents with preventive and / or therapeutic functions for METH addiction. This invention reveals that *Bifidobacterium pseudosporidis* has the advantages of high safety and strong targeting, and can achieve the prevention and intervention of METH addiction without complex treatment equipment, providing a novel biological treatment option for the clinical treatment of METH addiction.

The invention discloses an integrated treatment process for heavy metal wastewater. The integrated treatment process comprises the following steps: step 1, pretreatment; 2, biological agent treatment; step 3, air flotation and magnetic separation; step 4, precipitation and separation; step 5, post-processing; step 6, magnetic medium separation and recovery; wherein in the second step, a biological agent adding unit and a hydrolysis reaction unit are included, the biological agent treatment unit comprises a stirrer, an agent adding ring and a guide cylinder, the stirrer comprises a stirring motor and a stirring impeller, the stirring impeller is connected with the stirring motor and inserted into the guide cylinder, and the agent adding ring is connected with the stirring impeller and synchronously rotates with the stirring impeller; the flow guide cylinder is provided with a turbulent flow ring, the turbulent flow ring comprises a ring body and cutting teeth, the ring body is installed in the flow guide cylinder, and the cutting teeth are arranged on the ring body. Compared with the prior art, the purposes of efficient treatment, stable water outlet, land occupation and cost saving and convenience in installation and extension are achieved.

The invention discloses a novel antifreeze polypeptide with a clear structure and excellent performance and a protection method of cryopreserved cells. The core structure of the polypeptide is formed by covalently connecting two functional modules, the N end is a unit with ice crystal binding performance, the C end is a super-hydrophilic unit, and the two functional modules are bridged through a flexible linker. Experimental verification shows that the synthetic polypeptide shows excellent anti-freezing activity, can significantly inhibit nucleation, growth and recrystallization processes of ice crystals, and has particularly outstanding ice recrystallization inhibition activity. The novel antifreeze polypeptide shows excellent biocompatibility, and is suitable for low-temperature cryopreservation of cells sensitive to freezing damage and other living microbial preparations. Results show that the polypeptide can greatly improve the freeze-drying survival rate of cells and the activity of the cells after recovery, is non-toxic to the cells, and shows the potential of replacing a traditional cryoprotectant. Therefore, the method has wide application prospects in food industry, biological medicine, agricultural breeding and low-temperature preservation technologies of biological sample libraries.

The application discloses a specific nucleic acid aptamer for a heterogeneous core ribonucleoprotein hnRNPA2B1 and application, and belongs to the technical field of nucleic acid aptamer. The hnRNPA2B1 aptamer is a small single-stranded DNA screened from a designed oligonucleotide library through an in-vitro exponential enrichment ligand system evolution (SELEX), is composed of 80 nucleotides, and has high affinity to the target. The hnRNPA2B1 aptamer can be applied in a detection method or a detection product of the hnRNPA2B1 protein, and a targeted regulation drug or a biological preparation, and can be specifically made into a molecular probe, used as a detection reagent, used as a targeted preparation and the like for diagnosis and treatment of diseases related to the hnRNPA2B1 protein. The hnRNPA2B1 aptamer has strong affinity and specificity, has the advantages of low production cost, stability, convenient synthesis and easy modification compared with an antibody, and is a potential substitute for the antibody in disease diagnosis and treatment.

The invention provides application of an FZD4 gene. The FZD4 gene is used for regulating osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells. The FZD4 gene in the chicken bone marrow mesenchymal stem cells is over-expressed and is used for promoting osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; or / and the FZD4 gene in the chicken bone marrow mesenchymal stem cells is knocked down to inhibit osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; the nucleotide sequence of the coding region of the FZD4 gene is as shown in SEQ ID NO. 1. According to the invention, the positive regulation effect of the FZD4 gene in osteogenic differentiation of chicken bone marrow mesenchymal stem cells is determined for the first time, the blank of a bone regulation mechanism of the poultry FZD4 gene is filled, and a new biological preparation target is provided for prevention and treatment of poultry bone diseases; the FZD4 gene can also be used as a molecular marker for breeding chicken varieties with high bone strength.

The invention discloses a green algae expression method and application of recombinant human serum albumin, and belongs to the technical field of biology. Aiming at the problems of high cost and incomplete post-translational modification of the existing recombinant protein expression system, the invention provides a technical scheme for efficiently expressing human serum albumin by using green algae. The method comprises the following steps: carrying out codon optimization on a human serum albumin gene sequence, introducing a recombinant vector into green algae cells, and screening transgenic algae strains through a culture medium containing a resistance agent, culturing the transgenic algal strain, splitting the algal cells and separating the recombinant albumin. According to the method, efficient production of the human serum albumin is achieved through a green alga expression system, and the product has a correct folding structure and biological activity and is mainly used for preparing medical plasma substitutes, drug carriers and biological agents.

This invention provides a biological compound preparation for repelling insects or reducing the oviposition of flying insects, its preparation method, and its application. The biological compound preparation for repelling insects or reducing the oviposition of flying insects includes a chemical reagent and a biological agent; wherein the chemical reagent includes 2-phenylethanol, and the biological agent includes *Bacillus amyloliquefaciens* and *Bacillus brevis*; the preservation number of *Bacillus amyloliquefaciens* is CGMCC No. 35162, and the preservation number of *Bacillus brevis* is CGMCC No. 35161. The core advantage of this invention lies in the synergistic effect of 2-phenylethanol (chemical reagent) with *Bacillus amyloliquefaciens* (CGMCC No. 35162) and *Bacillus brevis* (CGMCC No. 35161) (biological agent). Through complementary and mutually reinforcing mechanisms, the two significantly enhance the control effect.

The invention discloses a potato late blight antagonistic bacterium and application thereof, belongs to biological agents, and provides more strain resources for prevention and treatment of potato late blight. The potato late blight antagonistic bacterium is Pseudomonas chlororaphis blT-49 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 27, 2021, and the preservation number is CGMCC No.24203. The invention further discloses a preparation method of the potato late blight antagonistic bacterium. The strain disclosed by the invention not only can inhibit the occurrence and growth of potato late blight, but also can regulate and control the ecological system of the micro-ecology of potatoes, reduce the infection ability of potato late blight and improve the disease resistance of potatoes. In the growth and development of the potatoes, the growth and development of the potatoes can be promoted, and the single-plant yield is improved.

The invention relates to a biological agent, in particular to a preparation method of the biological agent, which comprises the following steps: S1, injecting a cultured biological bacterium culture solution into a centrifugal space formed by a plurality of separation rings and a plurality of separation cavities; s2, a plurality of separation rings and a plurality of separation cavities are driven to rotate, and a plurality of water spraying holes spray liquid from top to top to impact the biological bacteria; s3, the biological bacteria with large mass are left on the lower side, and the biological bacteria with small mass are flushed to the upper side by the liquid; s4, after the biological bacteria are separated according to the mass, a plurality of separation rings rotate to discharge the liquid; s5, the biological bacteria are left in the centrifugal space; s6, pressure gas is introduced into a plurality of separation cavities, and an elastic inclined plate is inclined and expands to separate the biological bacteria according to the mass; and the biological bacteria can be centrifugally classified according to different masses.

The present disclosure provides materials and methods related to the purification of a biologic from a biological fluid. In particular, the present disclosure provides compositions, and related methods, comprising peptide ligands capable of removing process-related impurities (e.g., host cell proteins, nucleic acids, and media components) and product-related impurities (e.g., product fragments, product aggregates, and inactive forms derived from product degradation by or association with other species in the cell culture harvest) from biological fluids during the production of a biologic.