37 results about "Ustilaginoidea" patented technology

The invention discloses a special rice fertilizer containing efficient silicon and a preparation method of the special rice fertilizer, and belongs to the technical field of fertilizers in agricultural production. The fertilizer is prepared from the following raw materials in parts by weight: 40-55 parts of urea, 20-35 parts of calcium superphosphate, 10-25 parts of potassium chloride, 10-20 parts of potassium silicate, 1-5 parts of attapulgite powder, 0.2-1.2 parts of artemisia argyi extract, 0.2-0.8 part of radix peucedani extract and 0.5-1.5 parts of mint extract. The fertilizer disclosed by the invention is reasonable in nutrient matching, satisfies nutrient requirements and absorption characteristics of rice growth, and capable of promoting rice growth, increasing the rice yield, improving the lodging resistance and the insect resistance of the rice and lowering the lodging and insect pest risks. Besides, the traditional Chinese medicine extract in the fertilizer can be used for inhibiting the growth of rice sheath blight disease, rice blast and ustilaginoidea virens and lowering the rice disease harm. The fertilizer is applied once along with soil preparation before rice seedling transplanting, so that the utilization rate of the fertilizer is increased, and the fertilizer-application labor cost and fertilizer cost of the rice farmers are lowered.

The invention belongs to the technical field of plant protection, and particularly relates to an efficient stable indoor inoculation method for Ustilaginoidea virens and a special strain. The method includes: subjecting a hyphal mass (6mm) cultivated for 7 days on PSA (potato dextrose agar) to shake cultivation through PSB (photosynthetic bacteria) for 7-10 days so as to prepare hypha and spore mixed liquor, and filtering the mixed liquor with four layers of filter cloth to obtain spore liquor inoculants 106 / ml; inoculating rice ear bracts by injecting at the seventh stage of rice young ear differentiation; and keeping moist for three days at 25 DEG C and under 95%RH (relative humidity), and placing in a spray irrigation net chamber for cultivation at 25-32 DEG C and under 90%-98%RH. By the method, the rate of diseased ears under artificial inoculation of Ustilaginoidea virens can reach more than 90%. By the method compared with the outdoor artificial inoculation techniques, efficientstable incidence of Ustilaginoidea virens can be obtained under controllable conditions.

Trichoderma atroviride Ta-9 has the collection number of CGMCC No. 9698 and is collected at the CGMCC (China General Microbiological Culture Collection Center). The trichoderma atroviride Ta-9 disclosed by the invention can be used for preventing and controlling a variety of rice diseases as a biological pesticide. The trichoderma atroviride Ta-9 shows relatively strong superparasitism ability to rhizoctonia solani and ustilaginoidea virens. The invention further relates to trichoderma atroviride Ta-9 high-yield chlamydospore and conidiospore liquid fermentation technologies. After fermentation liquor of the trichoderma atroviride Ta-9 is diluted by 50 times, the diluted fermentation liquor has relatively good prevention and control action on both rice sheath blight disease caused by rhizoctonia solani and green smut caused by ustilaginoidea virens.

The invention relates to a PopW antibacterial protein and biological bactericide mixed biological preparation PopW-PF1, belonging to the technical field of plant protection. The total concentration of viable bacteria in a biological bactericide prepared from pseudomonas fluorescens is 1*10<9>CFU / ml; PopW antibacterial protein has the concentration of 30mg / ml and is diluted into 300-30000 times for use; and in the mixed biological preparation PopW-PF1, the PopW antibacterial protein and the biological bactericide have mixed volume ratio of (30000-30):1, and diluted into 50-100 times when in use. The mixed biological preparation PopW-PF1 has control effect reaching above 50 percent on viral diseases on vegetable crops of pepper, tomato and the like and grain crops of rice, bean and the like, and better control effect on other diseases on the ground such as leaf mold and ustilaginoidea virens, wherein the increase production effect reaches 10 percent.

The invention discloses penicillium capable of resisting false smut of rice and an application thereof. The penicillium is prepared through the following steps of: A, ustilaginoidea virens isolation, wherein a ustilaginoidea virens ear is collected, and the ustilaginoidea virens is isolated by adopting a chlamydospore suspension method; B, antibacterial isolation and culture of the ustilaginoideavirens, wherein a soil sample is ground, placed in an aseptic petri dish, and poured into a PDA (potato dextrose agar) culture medium to culture and isolate a single colony of fungus, the isolated fungus strain is inoculated into a PSA (prostate-specific antigen) culture solution for shaking culture, and the fermentation broth is filtered and degermed to obtain an aseptic supernatant sample; and C, antagonist screening of the ustilaginoidea virens, wherein a conidiophore suspension of the ustilaginoidea virens is coated on a PSA panel, an aseptic Oxford plate is placed in the panel, and the aseptic supernatant sample is added into the aseptic Oxford plate to culture penicillium. The invention also provides an application of the antagonist penicillium AF-25 in preparing a biological pesticide for controlling or preventing the false smut of rice. The penicillium has an antagonistic effect on ustilaginoidea virens, and can inhibit spore germination and mycelial growth of the ustilaginoidea virens. The fermentation broth of the penicillium has strong inhibition activity to the false smut.

The invention provides application of fusaric acid to inhibition of ustilaginoidea virens. A situation that fusaric acid is capable of effectively inhibiting mycelial growth and spore germination of the ustilaginoidea virens is found through a mycelial growth rate method and a spore germination bacterial colony inhibition method. A situation that the fusaric acid has a better control effect to ustilaginoidea virens is found through a field trial result. Dosages of effective constituents are 200mg / L, 300mg / L and 400mg / L, and average control effects of the effective constituents to the ustilaginoidea virens can respectively reach 48.40 percent, 54.92 percent and 63.08 percent. The first research of the application finds that the fusaric acid has strong inhibition function and good control effect to the ustilaginoidea virens, and provides a new scientific thought for further developing and researching and creating a novel agricultural bactericide and applying to the control of the ustilaginoidea virens.

The invention relates to a molecular marker of a new anti-ustilaginoidea virens gene of rice, belonging to the field of rice breeding for disease resistance. According to the invention, each strain of Teqing background backcross introgression lines obtained by the cross and backcross between a susceptible parent Teqing and a disease-resistant parent Lemont is subjected to evaluation of disease resistance and molecular genetic linkage analysis and positioned to the anti-ustilaginoidea virens gene qFSR10.5 on the 10th chromosome of rice; and the molecular marker linked with the qFSR10.5 is RM258. By checking the RM258 banding pattern data and screening the ustilaginoidea virens resistance controlled by the qFSR10.5 resistance allele, the breeding efficiency of the anti-ustilaginoidea virens rice variety can be improved.

The invention discloses a ribose culture medium applicable to ustilaginoidea virens spore production and an application method of the ribose culture medium. Ribose which is not sterilized at high temperature is adopted to prepare a potato ribose culture medium as a spore production culture medium for culturing and preparing ustilaginoidea virens thin-wall conidia. The preparation and application method of the culture medium comprises the following operation steps: 1) preparing a basic culture medium; 2) filtering and sterilizing ribose; 3) preparing a ribose culture medium plate; 4) planting ustilaginoidea virens; 5) performing constant-temperature culture; and 6) collecting a new generation of spores. The ribose culture medium has the characteristics and advantages that the time for obtaining a great amount of spores can be shortened, the continuous spore production time is long, operation techniques are simple, convenient and rapid, no oscillation culture equipment is needed, a relatively great number of spores of the new generation can be produced, and a spore liquid is relatively pure.

The invention relates to wettable powder of a trichoderma asperellum DQ1 composition and application thereof. The wettable powder of the trichoderma asperellum DQ1 composition is prepared from the following methods: one, preparing the trichoderma asperellum DQ1 strain as spore liquid to culturing; two, manufacturing the spore suspension, wherein the spore suspension is used as solid tray fermentation seed liquid; three, mixing the sterilized fermentation substrate and the solid tray fermentation seed liquid, culturing, air-drying, and crushing to obtain the trichoderma asperellum DQ1 spore powder; four, mixing the trichoderma asperellum DQ1 spore powder, the validamycin with raw powder content of 60%, and 5000UI / microgram bacillus thuringiensis in mass ratio of 10:(0.5-1):(0.5-1) to prepare the trichoderma asperellum DQ1 composition; and five, preparing the trichoderma asperellum DQ1 as the wettable powder. The wettable powder disclosed by the invention can effectively play the pesticide effect of the biopesticide, and can inhibit and kill banded sclerotial blight and ustilaginoidea virens and other plant diseases and insect pests, is non-toxic to the human and the animal and freefrom environment pollution.

The invention relates to a sterilization composition containing pyraclostrobin and nitrapyrin bacteria ester, and the effective ingredients are the pyraclostrobin and the nitrapyrin bacteria ester in a compound way. The mass ratio of the pyraclostrobin to the nitrapyrin bacteria ester is (1-80):(80-1), and in the preparation, the total mass of the effective ingredients (the pyraclostrobin and the nitrapyrin bacteria ester) is 1%-80% of the total mass of the whole preparation, and the balance is auxiliary components allowed to be used and acceptable in a pesticide. A pesticide formulation which can be prepared by using the sterilization composition can be milk oil, a suspending agent, wettable powder, a water dispersible granule, emulsion in water and microemulsion. The sterilization composition is mainly used for preventing and curing wheat powdery mildew, rice blast, ustilaginoidea virens, cucurbites powdery mildew, tomato powdery mildew, apple rust, watermelon anthracnose and flower powdery mildew, and has good preventing and curing effects on cucumber downy mildew, macrophoma musae and leaf spot diseases.

The invention discloses a drying and sealed preservation method of rice ustilaginoidea virens. The method technologically utilizes the property that the ustilaginoidea virens can survive in completely dried rice endosperm for a long period; rice is used as a carrier and the ustilaginoidea virens is artificially inoculated onto sterile rice grains and hyphae fully cover endosperm tissues of the rice grains; after the rice grains are completely dried under the condition of 28 DEG C, a drying preservation manner is adopted. The method comprises the following technical steps: 1) obtaining purified ustilaginoidea virens strains by utilizing a chlamydospore single-spore isolation method; 2) immersing the rice grains until the endosperm is fully filled with water; loading the endosperm into a test tube and sterilizing; 3) inoculating the endosperm onto the sterile rice grains and culturing to enable the hyphae fully cover the inner parts of the endosperm tissues; drying until the rice grains are completely dried, preserving the test tube into a dryer filled with allochroic silica gel and preserving for a long period; smearing vaseline for sealing. The drying and sealed preservation method has the advantages that the preservation time of the ustilaginoidea virens exceeds 5 years; the survival rate is high; the pollution rate of the strains is low; the operation is convenient and a small space is occupied; the cost is low and energy sources are not consumed; the pollution to environment and residues are not caused.

The invention relates to the technical field of microorganism, and specifically relates to Bacillus velezensis SYQN-1 and application of the Bacillus velezensis SYQN-1 in ustilaginoidea virens. The preservation No. is 16566. The Bacillus velezensis can better prevent and treat ustilaginoidea virens. A result of a three-point confrontation culture method experiment shows that the Bacillus velezensis SYQN-1 can resist the ustilaginoidea virens within an inhibition zone of 31.67 mm.

The invention discloses a recombinant vector, a host cell and an application of an ustilaginoidea virens effector protein, and belongs to the technical field of biology. The recombinant vector is usedfor expressing an ustilaginoidea virens effect protein, and is obtained by inserting a coding gene of the ustilaginoidea virens effect protein to a position between EcoR I and Xho I restriction enzyme cutting sites of a pET-32a vector. The amino acid sequence of the ustilaginoidea virens effect protein is shown in the formula of SEQ ID NO: 1 in a sequence table and the nucleotide sequence of thecoding gene of the ustilaginoidea virens effect protein is shown in the formula of SEQ ID NO: 2 in the sequence table. According to the invention, the ustilaginoidea virens effect protein obtained byin vitro expression is sprayed on arabidopsis thaliana leaves so that the contents of active oxygen and callose in the arabidopsis thaliana can be obviously improved and the disease resistance of thearabidopsis thaliana to pathogenic bacteria Pseudomonas syringae pv.tomato (Pst) DC3000 can be improved. A novel way is provided for improving the plant resistance and inducing the plant defense reaction, and the recombinant vector has a wide application prospect in agricultural production.

The invention discloses a bactericide of Ustilaginoidea virens or a reagent for prevention and treatment of Ustilaginoidea virens. The active ingredients of the reagent are composed of (E, E, E)-N-methyl-2-[((((1-methyl-3-(2, 6-dichlorophenyl)-2-allyl)imino)oxy)methyl)phenyl]-2-methoxyimino acetamide and (RS)-3-chloro-4-[4-methyl-2-1H-1, 2, 4-triazole-1-ylmethyl)-1, 3-dioxylpentane-2-yl)phenyl-4-chlorophenyl ether that are in a mss ratio of 1-4:9-6 or 7:3. The reagent provided by the invention solves the drug use problem in actual rice production, and has wide application prospects in the field of agricultural bactericides. The application of novel agents and blending of reagents with different action mechanisms help delaying the generation of drug resistance, and the reagent has significant social benefits.

The invention discloses delftia lacustris and application thereof in rice false smut prevention and control. The bacterial strain is named as delftia lacustris ZJU-B1 with the preservation number being CCTCC NO: M 2017641 and the preservation date being October 30, 2017. According to the delftia lacustris ZJU-B1 obtained through separation provided by the invention, the occurrence of ustilaginoidea virens can be effectively prevented and controlled, the disease occurrence rate is reduced, the yield and the safety of rice are remarkably improved, and the healthy and pollution-free production ofthe rice is realized.

The invention discloses a preparation and cultivation method for ustilaginoidea virens thin-wall conidia capable of preventing pollution. A conical flask can be used for effectively obstructing the characteristics of common microorganisms, a common conical flask is adopted to manufacture a utensil for culturing spores, and a potato cane sugar culture medium is taken as a spore production culture medium to prepare and cultivate the high-quality ustilaginoidea virens thin-wall conidia. The preparation and cultivation method comprises the following steps: 1) cleaning the common conical flask for standby use; 2) taking the potato cane sugar culture medium as the spore production culture medium to prepare a conical flask type culture medium; 3) transplanting matrix thalluses; 4) carrying out spore production culture under a temperature condition of 28DEG C; 5) carrying out elution by bacteria free water to collect new-generation thin-wall conidia. The preparation and cultivation method has the following advantages: 1) shake cultivation equipment is not required, and obtained conidia liquid finished product contains few mycelia, culture media, thallus metabolism excretion products and the like; 2) after the conical flask is provided with a conventional bottle stopper, common microorganisms can be prevented from entering, the conical flask can be favorably prevented from being polluted by infectious microbes, so that pollution can be effectively prevented, and a high-quality spore finished product can be obtained; 3) an operation for collecting the spores in the conical flask is more convenient than the operation for collecting the spores in a culture dish. According to the protection of the conical flask, spore production bacterial colonies can be put for a period of time at will without pollution, and a spore production state is kept so as to be convenient to connect with a working procedure which needs spores.

The invention discloses lysinibacillus fusiformis H16 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Lysinibacillus fusiformis H16, and the preservation number of the Lysinibacillus fusiformis H16 is CCTCC (China Center for Type Culture Collection) NO: M 2021179. The Lysinibacillus fusiformis H16 disclosed by the invention can inhibit the growth of the Ustilaginoidea virens strain YY7850, which is an example that the Lysinibacillus fusiformis is found to have antagonism to the Ustilaginoidea virens pathogenic bacteria for the first time, and due to the fact that the strain H16 has the capacity of inhibiting the Ustilaginoidea virens pathogenic bacteria, the strain has the potential of being developed into a rice false smut biocontrol bacterium.

The invention discloses a preparation method of rice germ inhibitor coming from xylose. The method includes the following steps of firstly, preparing compound xylose into a xylose solution; secondly, heating the xylose solution in a high-pressure and high-temperature heating kettle to 126 DEG C, and keeping the temperature for 30 minutes; thirdly, taking the xylose solution out of the heating kettle, naturally cooling the xylose solution at a room temperature, and forming the rice germ inhibitor; fourthly, conducting antibacterial effect detection on the inhibitor, wherein the obtained inhibitor is prepared into a culture medium, ustilaginoidea virens is planted in and cultured under the appropriate temperature conditions, and it can be seen that the growth and development of ustilaginoidea virens can be strongly restrained through the product obtained by conducting high-temperature treatment on xylose. The method has the advantages that through the simple high-temperature treatment technology, restraining substances for restraining the growth and development of ustilaginoidea virens efficiently can be obtained from compound xylose.

The invention discloses an ustilaginoidea virens inhibitor from xylose. The ustilaginoidea virens inhibitor is from a xylose compound and is generated by carrying out high-temperature treatment on xylose. The ustilaginoidea virens inhibitor from xylose has the beneficial effects that the ustilaginoidea virens inhibitor from xylose has a strong effect of inhibiting growth and development of ustilaginoidea virens; the ustilaginoidea virens inhibitor from xylose can provide efficient and effective technical beginning and an efficient and effective technological approach for research and development of ustilaginoidea virens pesticide.

The invention discloses a pollution-reduction preparation and culture method of ustilaginoidea virens thin-wall conidia. The method is characterized by adopting a triangular flask as a preparation and culture utensil, adopting a potato culture-medium as a sporulation medium, and preparing and culturing the high-quality ustilaginoidea virens thin-wall conidia. The preparation and culture method comprises the following steps of (1) cleaning the triangular flask for standby use; (2) preparing the bottle type sporulation medium of the potato culture-medium; (3) transplanting maternal ustilaginoidea virens; (4) sporulation culturing at the proper temperature; (5) eluting and collecting a new generation of thin-wall conidia. The pollution-reduction preparation and culture method of the ustilaginoidea virens thin-wall conidia provided by the invention has the advantages that (1) a shaking culture device has no need to be use; a conidia liquid finished product contains less impurities; the preparation process is fast, high in efficiency, and large in conidia quantity; (2) the pollution can be efficiently reduced during the preparation and culture process; (3) with the protection of the triangular flask, a sporulation bacterial colony can be placed at will for a period of time without being polluted, a sporulation state is kept, and a working procedure requiring the conidia can be linked conveniently.

The invention discloses a high-efficiency novel synergistic composition for preventing and treating three new major diseases of rice. Main components of the high-efficiency novel synergistic composition comprise propiconazole and trifloxystrobin. According to the invention, the propiconazole and trifloxystrobin are combined according to an effective ratio, so that good inhibition effects on the hypha growth of rhizoctonia solani, ustilaginoidea virens and magnaporthe oryzae are achieved; wherein the ratio of propiconazole to trifloxystrobin is 1:1. 5, the EC50 values of the composition on rhizoctonia solani, magnaporthe oryzae and ustilaginoidea virens are 0.23 mg / L, 0.03 mg / L and 0.02 mg / L respectively and the co-toxicity coefficients of the compounds are 396.00, 160.13 and 149.85. The bactericidal composition has obvious synergistic effect, the field control effects are respectively up to 86.55%, 86.99% and 85.86%, the control effect is obviously higher than that of a single agent, the dosage of pesticides can be effectively reduced, various diseases can be treated, the drug resistance of germs can be delayed, the labor intensity can be effectively reduced, and the production cost can be saved.

The invention belongs to the technical field of plant protection, and particularly relates to an efficient stable indoor inoculation method for Ustilaginoidea virens and a special strain. The method includes: subjecting a hyphal mass (6mm) cultivated for 7 days on PSA (potato dextrose agar) to shake cultivation through PSB (photosynthetic bacteria) for 7-10 days so as to prepare hypha and spore mixed liquor, and filtering the mixed liquor with four layers of filter cloth to obtain spore liquor inoculants 106 / ml; inoculating rice ear bracts by injecting at the seventh stage of rice young ear differentiation; and keeping moist for three days at 25 DEG C and under 95%RH (relative humidity), and placing in a spray irrigation net chamber for cultivation at 25-32 DEG C and under 90%-98%RH. By the method, the rate of diseased ears under artificial inoculation of Ustilaginoidea virens can reach more than 90%. By the method compared with the outdoor artificial inoculation techniques, efficientstable incidence of Ustilaginoidea virens can be obtained under controllable conditions.

The invention relates to the field of pesticides, and provides a pesticide for preventing and treating rice false smut. The pesticide is characterized in that the mass ratio of tannin to water in thepesticide is 1:2. The tannin can effectively inhibit growth of ustilaginoidea virens, so that the tannin can be used for controlling occurrence of rice false smut, improving the yield of rice, protecting the environment, and reducing pollution of the ustilaginoidea virens toxin to rice during production, so that safety of people and livestock is protected.

The invention discloses a group of ustilaginoidea virens PCR detection primers. Specific detection targets of ustilaginoidea virens are found through a method for comparing genomes, and the detection primers are designed. Compared with traditional ustilaginoidea virens PCR detection primers, the detection primers have the advantages of being good in specificity, high in stability and high in sensitivity, and the false positive phenomenon is well controlled in the detection process; meanwhile, ustilaginoidea virens can be accurately detected, and good tools are provided for field detection of ustilaginoidea virens, circulation research of ustilaginoidea virens diseases, prediction of the ustilaginoidea virens diseases and the like.

The invention discloses a combined bactericide for preventing and controlling Ustilaginoidea virens, and belongs to the field of pesticides. The combined bactericide comprises 15-30 wt% of coumoxystrobin, 10-25 wt% of prochloraz, 5-12 wt% of tricyclazole, 5-35 wt% of assistants, and the balance of a filler. The above bactericides are compounded and complement with each other to achieve synergisticeffects, so compared with single agents, the combined bactericide has the advantages of obvious synergistic effect on the rice Ustilaginoidea virens, remarkable improvement of the prevention and control effect, long lasting period, effectiveness in delaying the pesticide resistance of the rice Ustilaginoidea virens to the single agents, replacement of a part of medium-high toxicity bactericides,reduction of the dosage, reduction of environmental pollution and residues of agricultural products, and reduction of the cost.