The invention relates to a sterilization composition containing pyraclostrobin and nitrapyrin bacteria ester, and the effective ingredients are the pyraclostrobin and the nitrapyrin bacteria ester in a compound way. The mass ratio of the pyraclostrobin to the nitrapyrin bacteria ester is (1-80):(80-1), and in the preparation, the total mass of the effective ingredients (the pyraclostrobin and the nitrapyrin bacteria ester) is 1%-80% of the total mass of the whole preparation, and the balance is auxiliary components allowed to be used and acceptable in a pesticide. A pesticide formulation which can be prepared by using the sterilization composition can be milk oil, a suspending agent, wettable powder, a water dispersible granule, emulsion in water and microemulsion. The sterilization composition is mainly used for preventing and curing wheat powdery mildew, rice blast, ustilaginoidea virens, cucurbites powdery mildew, tomato powdery mildew, apple rust, watermelon anthracnose and flower powdery mildew, and has good preventing and curing effects on cucumber downy mildew, macrophoma musae and leaf spot diseases.