Pollution-reduction preparation and culture method of ustilaginoidea virens thin-wall conidia

A rice smut fungus and culture method technology, applied in the field of phytopathology, can solve the problems of high pollution probability, bacterial contamination of culture plates, material contamination in petri dishes, etc., and achieve the effect of reducing pollution

Pending Publication Date: 2017-05-24
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The air around the culture dish is in a state of constant flow for sporulation culture in this type of incubator, and the probability of contamination of the materials in the culture dish is quite high. Usually, more than 50% of the culture plates will be contaminated by bacteria after 5 days of cultivation, even if some No obvious c

Method used

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Examples

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Effect test

preparation example Construction

[0029] The preparation and cultivation of conidia of rice false smut bacteria on agar medium all adopt conventional technical specifications, that is, the culture technology of using culture dish in combination with agar medium is used for preparation and cultivation; there is no use of triangular flasks as culture utensils so far. , The spore preparation technology of the spore-forming culture of Aspergillus oryzae on the agar medium. The possible reason is the long-term dependence and habitual use of the petri dish, and it is generally believed that the spore production of pathogenic bacteria requires a good aeration environment, and the petri dish can just meet the good aeration.

[0030] After common triangular flask is put on bottle stopper, though form the closed environment with poor ventilation, contriver repeatedly tests and finds, oryzae smut bacteria can form conidia on the potato agar medium in this comparatively closed environment; The application of the bottle ju...

Embodiment 1

[0039] Apply a kind of method for preparing and cultivating the conidia of the rice smut bacteria strain Uv-105 that reduces pollution of the present invention, prepare and cultivate the conidia of the rice smut bacteria strain Uv-105, and implement the operation as follows:

[0040] 1. Use a 250mL common triangular flask as a device for preparing spores, and clean the triangular flask for later use.

[0041]2. Preparation of bottle-type sporulation medium: use potato medium as sporulation medium, the proportion of this medium is: potato 100g, agar 20g, water 1000mL; subpackage the medium in the triangle flask prepared in step 1 , each bottle is filled with 30mL, and after conventional high-pressure high-temperature sterilization, it will become a medium in a triangular flask for future use.

[0042] 3. Transplantation of the mother rice false smut fungus: use the conidia liquid of the rice false smut bacteria strain Uv-105 as the mother generation thallus, and transplant 50 μ...

Embodiment 2

[0046] Apply a method for preparing and cultivating conidia of the rice smut bacteria strain Uv-110 by applying a method for preparing and cultivating the conidia of the rice smut bacteria strain Uv-110 that reduces pollution, and perform operations according to steps 1 to 5 of Example 1, the difference is only The bacterial strain of step 3 is Uv-110; As a result, the spores on a bottle of colonies were eluted with 15 mL of sterile water, and the concentration of spores obtained was 2.02×10 6 individual / mL, conidia liquid without contamination by bacteria.

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PUM

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Abstract

The invention discloses a pollution-reduction preparation and culture method of ustilaginoidea virens thin-wall conidia. The method is characterized by adopting a triangular flask as a preparation and culture utensil, adopting a potato culture-medium as a sporulation medium, and preparing and culturing the high-quality ustilaginoidea virens thin-wall conidia. The preparation and culture method comprises the following steps of (1) cleaning the triangular flask for standby use; (2) preparing the bottle type sporulation medium of the potato culture-medium; (3) transplanting maternal ustilaginoidea virens; (4) sporulation culturing at the proper temperature; (5) eluting and collecting a new generation of thin-wall conidia. The pollution-reduction preparation and culture method of the ustilaginoidea virens thin-wall conidia provided by the invention has the advantages that (1) a shaking culture device has no need to be use; a conidia liquid finished product contains less impurities; the preparation process is fast, high in efficiency, and large in conidia quantity; (2) the pollution can be efficiently reduced during the preparation and culture process; (3) with the protection of the triangular flask, a sporulation bacterial colony can be placed at will for a period of time without being polluted, a sporulation state is kept, and a working procedure requiring the conidia can be linked conveniently.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a method for preparing and cultivating conidia of the rice false smut fungus parenchyma with reduced pollution. Background technique [0002] Rice false smut is an important disease of rice, which can cause great losses to rice production. The disease is caused by the infection of rice smut fungus (Ustilaginoidea virens). Conidia are the asexual propagules of rice false smut, which play an important role in the disease cycle and epidemic of rice false smut. In the laboratory, conidia are also indispensable materials for the study of rice false smut. Studies on the formation and development physiology of spores, the survival adaptability or resistance to adverse environments, and the recognition and interaction between spores and hosts all require the use of materials in the form of spores; research on the development of new prevention and control drugs, such as pharma...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/645
Inventor 张君成张丽丽王忠文
Owner GUANGXI UNIV
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