Pollution-reduction preparation and culture method of ustilaginoidea virens thin-wall conidia

Abstract

The invention discloses a pollution-reduction preparation and culture method of ustilaginoidea virens thin-wall conidia. The method is characterized by adopting a triangular flask as a preparation and culture utensil, adopting a potato culture-medium as a sporulation medium, and preparing and culturing the high-quality ustilaginoidea virens thin-wall conidia. The preparation and culture method comprises the following steps of (1) cleaning the triangular flask for standby use; (2) preparing the bottle type sporulation medium of the potato culture-medium; (3) transplanting maternal ustilaginoidea virens; (4) sporulation culturing at the proper temperature; (5) eluting and collecting a new generation of thin-wall conidia. The pollution-reduction preparation and culture method of the ustilaginoidea virens thin-wall conidia provided by the invention has the advantages that (1) a shaking culture device has no need to be use; a conidia liquid finished product contains less impurities; the preparation process is fast, high in efficiency, and large in conidia quantity; (2) the pollution can be efficiently reduced during the preparation and culture process; (3) with the protection of the triangular flask, a sporulation bacterial colony can be placed at will for a period of time without being polluted, a sporulation state is kept, and a working procedure requiring the conidia can be linked conveniently.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a method for preparing and cultivating conidia of the rice false smut fungus parenchyma with reduced pollution. Background technique [0002] Rice false smut is an important disease of rice, which can cause great losses to rice production. The disease is caused by the infection of rice smut fungus (Ustilaginoidea virens). Conidia are the asexual propagules of rice false smut, which play an important role in the disease cycle and epidemic of rice false smut. In the laboratory, conidia are also indispensable materials for the study of rice false smut. Studies on the formation and development physiology of spores, the survival adaptability or resistance to adverse environments, and the recognition and interaction between spores and hosts all require the use of materials in the form of spores; research on the development of new prevention and control drugs, such as pharma...

AI Technical Summary

Problems solved by technology

The air around the culture dish is in a state of constant flow for sporulation culture in this type of incubator, and the probability of contamination of the materials in the culture dish is quite high. Usually, more than 50% of the culture plates will be contaminated by bacteria after 5 days of cultivation, even if some No obvious colonies of penicillium and other contaminating bacteria can be seen on the culture plate, but the probability of contamination that is invisible to the naked eye is still relatively high

Therefore, it is technically difficult to rule out the possibility of contamination by using a petri dish to prepare and cultivate conidia of rice smut