35 results about "Smut fungus" patented technology

The invention relates to the field of biology breeding and in particular relates to an agrobacterium-mediated ustilago esculenta (Ustilago esculenta) transformant strain as well as a preparation method and application thereof. According to the agrobacterium-mediated ustilago esculenta transformant strain, an agrobacterium infected receptor of the transformant strain refers to ustilago esculenta (Ustilago esculenta). The method for preparing the agrobacterium-mediated ustilago esculenta transformant strain comprises the following steps: (1) preparing a fungal expression vector; (2) preparing agrobacterium competence; (3) performing electrotransformation on the agrobacterium; and (4) performing agrobacterium-mediated transformation, thereby obtaining the transformant. A complex protoplast is not needed, the receptor source is simple, and spores and hypha of the fungi can be directly used for transformation; and the transformation efficiency is high. In addition, the obtained transformant is large in single-copy ratio, and marker genes are conveniently obtained.

The invention discloses an ustilago esculenta teliospore formation related gene Itd1 and an application thereof and belongs to the technical field of molecular biology. On one hand, the key gene Itd1promoting formation of field zizania latifolia infected with sporidial strain of ustilago esculenta and ustilago esculenta teliospore is provided, on the other hand, the application of the key gene Itd1 to normal pregnant zizania latifolia is provided, and a new direction is provided for zizania latifolia breeding.

The invention discloses a method for improving yield of fruiting bodies of cordyceps takaomontana. The method comprises the steps of activating an ustilago esculenta GZUIFR-DX091 bacterial strain by applying a PDA culture medium, picking three loops for the activated bacterial strain by applying an inoculating loop to access into the ustilago esculenta culture medium, centrifuging culture liquor for 5 minutes at 5000r / min after culturing for 5 days at 28 DEG C at 160r / min, and getting liquid supernatant to obtain fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain; infecting silkworms with cordyceps takaomontana, and starting producing fruiting bodies for later use when the infected silkworms are hardened; pre-sterilizing a culture container, and tiling single layer or double layers of sterilized water-absorbing paper at the bottom of the container; slowly adding the prepared fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain onto the water-absorbing paper at the bottom of the container, placing the infected silkworms into a container which is added with the cane shoot fermentation liquor, and carrying out culture under light for 12 hours at 20-23 DEG C, wherein the culture temperature can be lowered by 5 DEG C for culturing at a dark culture stage. In the culture process, if the added fermentation liquor is dried, the fermentation liquor is needed to be timely complemented; the fruiting bodies of cordyceps takaomontana can be harvested after culturing for 3-4 weeks. The method disclosed by the invention can greatly improve the yield of the fruiting bodies of cordyceps takaomontana.

This invention addresses the problem of providing an agent for controlling disease induced in a plant by a bacterium selected from the genus Rhizoctonia, the genus Fusarium, the genus Pythium, the genus Microdochium, the genus Tilletia, the genus Ustilago, the genus Streptomyces, the genus Erwinia, and the genus Aphanomyces, the plant being selected from cruciferous plants, beans, corn, wheat, potatoes, and sugar beets. In the present invention, this disease can be controlled by treating seeds, seedlings, or soil of these plants using a fungus belonging to the genus Talaromyces.

The invention discloses a Ustilago-maydis haploid strain UM02. The collection number of the Ustilago-maydis haploid strain UM02 is CGMCC No.15387. The Ustilago-maydis haploid strain UM02 is combined with a haploid strain matched with the Ustilago-maydis haploid strain UM02 in mating type, then the pathogenicity is high, the success rate of inoculation is high, an identification result of the ustilago maydis resistance of maize varieties is accurate and reliable, and the consistency is good. When the Ustilago-maydis haploid strain UM02 is used for maize mushroom production, the yield of maize mushrooms is high, husk leaves are wrapped with the Ustilago-maydis haploid strain UM02, and harvesting and transportation are convenient.

The invention provides a strain of Bacillus sp. and its fermentation product and application, and relates to the field of microbiological technology. The bacterium is named Bacillus sp. IMDGX0108, and the preservation number is CCTCC NO: M 2018067; the preservation location is: China, Wuhan, Wuhan University , China Typical Culture Collection Center, the preservation time is January 30, 2018, the fermentation product of this strain is rich in 24-membered macrolide, and its fermentation product has a strong inhibitory effect on sugarcane smut , the fermentation product can be applied to inhibit Ustilago cane whip and be used to prepare biological pesticides capable of inhibiting Ustilago cane whip, and has a good prospect in the development and research of biopesticides.

The invention discloses a haploid strain UM02 of Ustilago maydis, and its preservation number is CGMCC No.15387. The haploid strain UM02 of maize smut haploid strain UM02 of the present invention is combined with the haploid strain matched with its mating type to have strong pathogenicity, high success rate of inoculation, accurate and reliable identification results of maize variety resistance to maize smut resistance, and Good consistency; when used in the production of corn mushrooms, not only the yield of corn mushrooms is high, but also wrapped in bracts, which is convenient for harvesting and transportation.

The invention relates to a haploid strain of Ustilago smut UET1 and its application, belonging to the field of biotechnology. The smut fungus ( Ustilago esculenta ) haploid strain UET1, the preservation number is: CGMCC No.11843. Zizania artificial breeding requires the co-infection of two sexually compatible haploid strains. This strain can be used as the mother strain of Zizania artificial breeding. Artificially pregnant watermelon. At the same time, the haploid strain can be genetically engineered to provide basic materials for the subsequent improvement of Zizania varieties, such as improving the environmental adaptability of Zizania, controlling the time of mating and so on.

The invention discloses a pair of Ustilago maydis combination, including Ustilago maydis haploid strain UMO1 and Ustilago maydis haploid strain UMO2; wherein the preservation number of UMO1 is CGMCC No.15263; the deposit number of the UM02 is CGMCC No.15387. The maize smut combination of the present invention has strong pathogenicity, high inoculation success rate, accurate and reliable identification results of resistance to maize smut resistance of maize varieties, and good repeatability and consistency; when used in the production of maize mushrooms, not only The corn mushroom yield is high, and the corn mushroom is wrapped in bracts, which is convenient for harvesting and transportation.

The invention relates to the technical field of microbiology, in particular to ustilago sp. HFJ311 and an application thereof to promotion of plant growth and improvement of tolerance of plants to various stresses. The ustilago sp. HFJ311 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the ustilago sp. HFJ311 is CGMCC No. 18150. The ustilago sp. can promote the growth of overground parts and underground parts of plants and enhance tolerance of the plants to salt, drought and stress of various metal ions. The ustilago sp. can reduce the damage of cadmium ion stress to the plants by promoting the chlorophyll content of plant seedlings, improving in-vivo antioxidant enzyme activity, reducing cadmium ion accumulation and the like. The ustilago sp. HFJ311 can be used for preparing a preparation for promoting plant growth and a soil conditioner, and has a wide application prospect.

The invention discloses a recombinant lipase mutant, gene, carrier, engineering bacterium and application thereof. The mutant is obtained from Sporisorium reilianum SRZ2 lipase (SRL) through mutation. In the present invention, the lipase mutant is obtained by performing single or combined mutations at positions 145 and 194 of the amino acid sequence shown in SEQ ID NO.2. Compared with the wild type enzyme, the catalytic activity and substrate tolerance of the mutant are greatly improved when transforming the above reaction, and the reaction process time is significantly shortened. Compared with the preparation of (S,S)-2,8-diazabicyclo[4,3,0]nonane by chemical method, the product obtained by the technology provided by the present invention has high stereoselectivity, milder reaction conditions, and less equipment requirements Low, reduces the reaction cost, and is friendly to the environment.

The invention discloses an endogenous strong promoter pHSP of smut smut and its expression vector and application, belonging to the technical field of genetic engineering. The present invention includes: a strong endogenous promoter pHSP of Ustilago smut, the nucleotide sequence of which is shown in SEQ ID NO.1; an expression vector containing strong endogenous promoter pHSP of Ustilago smut; Driving the transcription and expression of the eGFP gene, constructing a stable expression system and obtaining the application of engineering smut smut has improved eGFP expression intensity and fluorescence stability. The present invention combines endogenous pHSP promoter and strong terminator nosT of Ustilago smut with eGFP The genes are connected to construct an effective plasmid vector pUe-cbx-Hsp, which improves eGFP The expression intensity of the start eGFP Compared with otef and TFIID2, the gene transcription level was enhanced by more than 9 times and 11 times respectively.

The invention belongs to the technical field of prevention and control on crop diseases and pests, and particularly discloses a method for improving resistance of sugarcane on smut. The method comprises the following steps of before the sugarcane is planted, applying soluble silicate into silicon-lack soil, so that the content of effective silicon in the soil is 230 to 430mg / kg; uniformly mixing water and the soil, enabling the soil to react, and finally planting the sugarcane, so as to obviously reduce the generation rate of sugarcane smut, improve the resistance of the sugarcane on the smut,and promote the growth of the sugarcane. The soluble silicate can be applied to prevent and control the sugarcane shimadae and sugarcane smut. Compared with the traditional disease-resisting, seed-breeding and chemical agent-preventing and controlling methods, the method has the advantages that the safe and non-poisonous effects are realized, the green and environment-friendly effects are realized, and the operability is high; the method can be widely popularized and applied into agricultural production.

The invention discloses new applicationfor fungicidal synergism of farnesol and triazole fungicides in prevention and treatment of smut of sugarcane, corn and other cropscaused by sporisorium and ustilago. Farnesol and triazole fungicides have obvious synergistic effects,can greatly reduce the use of chemical pesticides, have the prevention and treatment effect obviously superior to that of a single agent, and thus have a good application prospect.

The invention discloses an Ustilago esculenta typing identification method and application thereof, and belongs to the technical field of molecular biology. The invention provides a target for typing identification of Ustilago esculenta. The target is derived from T-type Ustilago esculenta, and the nucleotide sequence of the target is as shown in SEQ ID No. 1. According to the Ustilago esculenta typing identification method provided by the invention, T-type Ustilago esculenta in a complex sample can be quantitatively detected, the detection limit is as low as 0.0088 ng / [mu] L (22.36 copies / [mu] L), and meanwhile, the quantitative detection of the T-type Ustilago esculenta in different tissues in different stages of plants is realized.

The invention relates to the field of genetic engineering and in particular relates to an ustilago maydis sucrose transportation protein UmSRT1 gene, an expression vector and an application of the expression vector in increase of cotton yield. According to the invention, a specific promoter and the ustilago maydis sucrose transportation protein UmSRT1 gene are fused, a plant expression vector specifically expressing a sucrose transportation protein gene is constructed, the plant expression vector is transformed into a host, so that a transformant is obtained, then the host is used for transforming a plant, and obtained transgenic cotton specifically expressing the ustilago maydis sucrose transportation protein UmSRT1 gene can be improved in yields of unginned cotton and ginned cotton of cotton.

The invention discloses an ustilago esculenta endogenous promoter pEF as well as an expression vector and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the ustilago esculenta endogenous promoter pEF is shown as SEQ ID NO.1. The invention discloses the expression vector containing the ustilago esculenta endogenous promoter pEF, an application of the ustilago esculenta endogenous promoter pEF to driving transcription and expression of an eGFP gene, constructing a stable expression system and obtaining engineering ustilago esculenta, and an application of the ustilago esculenta endogenous promoter pEF to improving expression intensity and fluorescence stability of eGFP. The ustilago esculenta endogenous pEF promoter and a strong terminator nosT are connected with the eGFP gene, an effective plasmid vector pUe-cbx-EF is constructed, more choices are provided for construction of an ustilago esculenta genetic transformation vector, and a foundation is laid for functional gene research of the ustilago esculenta.

The invention discloses a Ustilago maydis haploid strain UMO1, and its preservation number is CGMCC No.15263. The haploid strain UMO1 of maize smut haploid strain UMO1 of the present invention is combined with the haploid strain matched with its mating type to have strong pathogenicity, high success rate of inoculation, accurate and reliable identification results of maize variety resistance to maize smut resistance, and Good consistency; when used in corn mushroom production, the yield of corn mushroom is high, and it is easy to harvest and transport.