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Ustilago sp. and application thereof to promotion of plant growth in normal and stress environments

A technology that promotes plant growth and smut, applied in the field of microbiology, can solve problems such as not having the ability to promote vegetable growth, and has not yet discovered the stress ability, so as to achieve the effect of alleviating damage and broad application prospects

Active Publication Date: 2021-01-05
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Azospirillum brasiliensis does not have the function of promoting the growth of vegetables under normal conditions, and the effect of this bacteria on the ability of plants to tolerate various stresses such as salt, drought and other metal ions has not been found.

Method used

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  • Ustilago sp. and application thereof to promotion of plant growth in normal and stress environments
  • Ustilago sp. and application thereof to promotion of plant growth in normal and stress environments
  • Ustilago sp. and application thereof to promotion of plant growth in normal and stress environments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1. Isolation and Identification of Bacterial Strains

[0031] PDA medium configuration: 200 grams of potatoes, 20 grams of glucose, 15-20 grams of agar; 1000 ml of distilled water, natural pH.

[0032]Isolation method of the strain: the strain is isolated from the rhizosphere environment of Arabidopsis thaliana, and a single colony is obtained by streaking on the PDA medium.

[0033] The isolated strains were identified based on the ITS gene sequence of the strain. The fungal universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') were used to amplify the ITS gene of the strain. After the PCR products were recovered and sequenced, the obtained sequences were BLAST compared in the NCBI database and a phylogenetic tree was constructed, such as figure 1 As shown, it was determined that the isolated bacterial strain belonged to the genus Ustilago sp., and was named Ustilago sp. HFJ311.

[0034] The smut fungus (Ustilago sp.) HF...

Embodiment 2

[0035] Embodiment two, the influence of smut fungus (Ustilago sp.) HFJ311 on plant growth

[0036] 1. 1 / 2MS medium formula:

[0037] (1) A large number of element mother liquid configuration (20×): Accurately weigh 33g NH 4 NO 3 , 38g KNO 3 , 8.8g CaCl 2 2H 2 O, 7.4g MgSO 4 ·7H 2 O and 3.4 g KH 2 PO 4 . Dissolve the above-mentioned medicines in deionized water respectively, and then make the volume to 1000ml.

[0038] (2) Trace element mother liquid configuration (200×): Accurately weigh KI 0.166g, H 3 BO 3 1.24g, MnSO 4 4H 2 O4.46g, ZnSO 4 ·7H 2 O 1.72g, Na 2 MoO 4 2H 2 O 0.05g, CuSO 4 ·5H 2 O 0.005g and CoCl 2 ·6H 2 O0.005g, the above-mentioned medicines are dissolved in deionized water respectively, and the volume is set to 1000ml.

[0039] (3) Iron salt mother liquor configuration (200×): Accurately weigh FeSO 4 ·7H 2 O 5.56g, Na 2 EDTA·2H 2 O 7.46g were dissolved, and the volume was adjusted to 1000ml.

[0040] 1 / 2MS medium preparation method:...

Embodiment 3

[0046] Embodiment three, the alleviating effect of smut fungus (Ustilago sp.) HFJ311 on the growth of Arabidopsis under salt stress and drought stress

[0047] 1. Preparation of Salt Stress Medium and Drought Stress Medium

[0048] The preparation of 1 / 2MS medium is the same as in Example 2.

[0049] The medium used in the experiment was 1 / 2MS medium; the salt stress medium was simulated by adding 75mM NaCl to 1 / 2MS medium; the drought stress medium was simulated by adding 100mM Mannitol (Mannitol) to 1 / 2MS medium; after high temperature sterilization ,spare.

[0050] 2. Co-cultivation treatment of Ustilago sp. HFJ311

[0051] Control group: Transplant germinated aseptic seedlings of Arabidopsis thaliana into 1 / 2MS medium, salt stress medium and drought stress medium respectively, place them in a plant artificial climate chamber, cultivate them for 15 days, and measure the aboveground growth of Arabidopsis thaliana and underground fresh weight.

[0052] Experimental group:...

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Abstract

The invention relates to the technical field of microbiology, in particular to ustilago sp. HFJ311 and an application thereof to promotion of plant growth and improvement of tolerance of plants to various stresses. The ustilago sp. HFJ311 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the ustilago sp. HFJ311 is CGMCC No. 18150. The ustilago sp. can promote the growth of overground parts and underground parts of plants and enhance tolerance of the plants to salt, drought and stress of various metal ions. The ustilago sp. can reduce the damage of cadmium ion stress to the plants by promoting the chlorophyll content of plant seedlings, improving in-vivo antioxidant enzyme activity, reducing cadmium ion accumulation and the like. The ustilago sp. HFJ311 can be used for preparing a preparation for promoting plant growth and a soil conditioner, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of microbiology, in particular to Ustilago sp. HFJ311 and its application for promoting plant growth and improving plant tolerance to salt, drought stress and various metal ion stresses. Background technique [0002] High salinity and drought stress are great challenges to plant growth, directly affecting plant growth and development, and reducing food crop yields. The development of modern agriculture and the extensive use of chemical fertilizers have made soil salinization an important factor restricting the development of global agriculture, leading to serious social, economic and environmental problems. According to statistics, the global salinized land area is about 960 million hectares, and my country has about 100 million hectares. Soil salinization reduces soil compaction and fertility, which is not conducive to plants absorbing nutrients and hinders crop growth and production. In addition, drought...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00C09K17/14C12R1/645
CPCA01N63/30C09K17/14C12R2001/645C12N1/145
Inventor 毕玉蓉王圣旺纳小凡王晓敏梁翠芳杨磊
Owner LANZHOU UNIVERSITY
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