12 results about "Tilletia" patented technology

The invention discloses a hybridoma cell strain 1D6 as well as a monoclonal antibody and application thereof. The invention also discloses an application of the monoclonal antibody for resisting the Tilletia foetida in the detection of teliospore of the Tilletia foetida. The invention also discloses a single base sequence DNA single chain modified Tilletia foetida monoclonal antibody. The invention also discloses a detection kit. The monoclonal antibody as well as the marker and the kit thereof show high specific recognition capability on Tilletia foetida. The method for detecting the single base by mass spectrometry has a signal amplification effect and is simple and convenient in detection operation, so that the sensitivity and the detection efficiency of the method are remarkably improved. With the progress and continuous popularization and application of the mass spectrum technology, the technology has wide application potential and prospect.

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The present application discloses a microfluidic chip for detecting quarantine pathogens of raw grains. Each microfluidic chip of the present application includes at least 8 detection channels, and each detection channel includes at least 48 reaction zones, and the 48 reaction zones are used for detecting 11 kinds of quarantine pathogens of raw grains; the 11 kinds of quarantine pathogens of raw grains include **Diaporthe phaseolorum var. caulivora**, **Phialophora gregata**, **Fusarium virguliforme**, **Curtobacterium flaccumfaciens pv. flaccumfaciens**, **Tilletia indica**, **Tilletia walkeri**, **Wheat streak mosaic virus**, **Xanthomonas stewartii subsp. stewartii**, **Clavibacter michiganensis subsp. nebraskensis**, **Maize chlorotic mottle virus** and **Clavibacter michiganensis subsp. michiganensis**. The microfluidic chip of the present application can simultaneously detect 11 kinds of quarantine pathogens of raw grains for 8 samples to be tested, improves the detection efficiency, and has important significance for the inspection and quarantine work of quarantine pathogens of raw grains. **Note**: The scientific names of the pathogens are provided in Latin for accuracy in the technical field, and they can be adjusted according to specific requirements or common usage in the relevant industry. If you need to use more common or simplified names, please let me know and I can modify accordingly.

The invention discloses a method for detecting Tilletia foetida spores by combining ICP-MS with nanogold, and belongs to the technical field of biological detection.The method comprises the steps that a biotin solution reacts with a Tilletia foetida spore polyclonal antibody to obtain a biotin-labeled polyclonal antibody, streptavidin magnetic beads are added, incubation is conducted to obtain immunomagnetic beads, chloroauric acid is added into deionized water, and the immunomagnetic beads are added into the deionized water; the method comprises the following steps: adding sodium citrate after boiling to obtain a nano-gold solution, uniformly mixing the nano-gold solution with a Tilletia foetida spore monoclonal antibody, adding BSA, centrifuging to obtain a nano-gold probe, closing immunomagnetic beads, adding a sample solution containing Tilletia foetida spores, and performing magnetic separation to obtain a spore-magnetic bead compound; the method comprises the following steps: sealing a nanogold probe with a skim milk powder solution, adding a spore-magnetic bead compound, performing magnetic separation to obtain a magnetic bead-spore-nanogold compound, adding a citric acid solution into the magnetic bead-spore-nanogold compound, performing desorption to obtain a desorption solution, and introducing the desorption solution into ICP-MS for detection, so that the sensitivity is high, and the detection is rapid.

The invention discloses a hybridoma cell strain 1B5 as well as a monoclonal antibody and application thereof. The invention also discloses an application of the monoclonal antibody for resisting the Tilletia foetida in the detection of teliospore of the Tilletia foetida. The invention also discloses a monoclonal antibody of the Tilletia foetida labeled by the SERS nano-label. The invention also discloses a detection kit. The monoclonal antibody as well as the marker and the kit thereof show high specific recognition capability on Tilletia foetida. The Raman spectrometer is adopted to detect the nano-label signal, so that the detection operation is simple and convenient, the sensitivity and the detection efficiency of the method are remarkably improved, and quantitative analysis can be performed according to the strength of the Raman spectrum signal.

The present invention discloses a hybridoma cell line 1B5, its monoclonal antibody and applications. The present invention also discloses the application of the monoclonal antibody against Tilletia foetida in the detection of Tilletia foetida teliospores. The present invention also discloses a SERS nanolabel for labeling the monoclonal antibody against Tilletia foetida. The present invention also discloses a detection kit. The monoclonal antibody, its label and the kit exhibit a high specific recognition ability for Tilletia foetida. Detecting the nanolabel signal using a Raman spectrometer has the advantages of simple detection operation, which significantly improves the sensitivity and detection efficiency of the method. Quantitative analysis can also be performed according to the intensity of the Raman spectral signal.

The invention relates to tilletia foetida, in particular to a tilletia foetida effect protein TlCDA and application thereof. The invention provides an effect protein of tilletia foetida, and the amino acid sequence of the effect protein is shown as SEQ ID NO: 2. The effector protein can effectively inhibit programmed death of plant cells induced by BAX, and plays an important role in inhibiting a plant defense reaction process. The invention lays a foundation for deeply researching the pathogenesis of the tilletia foetida, excavating the tilletia foetida-resistant gene of the wheat and developing the tilletia foetida-resistant wheat variety.

The application discloses a wheat Tilletia indica-specific biomarker combination, a screening method and application thereof, and the biomarker combination comprises 20-hydroxyarachidonic acid, 1-octadecyl lysophosphatidic acid, linolenic acid, 13-hydroxydocosahexaenoic acid, 4-methoxy estrone, hypoxanthine, 3-hydroxymyristic acid, beta-ecdysterone, 17-phenyltrinorprostaglandin F2 alpha cyclopropylmethylamide, 11,12-dihydroxyeicosatrienoic acid and oxidized octadecadienoic acid. When the biomarker combination is used for identifying Tilletia indica, the time-consuming long and the accuracy easily interfered by the spore development stage limitations of traditional morphological identification are overcome; in view of the molecular level identification difficulty caused by the high genome homology of Tilletia indica and Tilletia controversa, the application digs the feature difference at the level of metabolomics, and constructs a specific chemical fingerprint, so that the technical bottleneck that the close relatives are difficult to accurately distinguish is effectively solved.

The present invention discloses a hybridoma cell line 1D6, its monoclonal antibody and applications. The present invention also discloses the application of the monoclonal antibody against Tilletia foetida in the detection of Tilletia foetida teliospores. The present invention also discloses a single-stranded DNA with a single-base sequence for modifying the monoclonal antibody against Tilletia foetida. The present invention also discloses a detection kit. The monoclonal antibody, its labeling agent and the kit exhibit a high specific recognition ability for Tilletia foetida. The method of detecting single bases by mass spectrometry has a signal amplification effect, and the detection operation is simple, so that the method has been significantly improved in terms of sensitivity and detection efficiency. With the progress and continuous popularization and application of mass spectrometry technology, this technology has broad application potential and prospects.

The invention belongs to the technical field of gene engineering of enzymes, and particularly relates to lipase as well as preparation and application thereof. The wild type lipase TWG5L coding gene from Tilletia walkeri is obtained through whole gene synthesis, and the wild type lipase and the coding gene twg5l of the wild type lipase are obtained. A wild type lipase gene is modified by using a key site amino acid residue site-directed mutagenesis technology to obtain a lipase mutant TWG5LN87Y and an encoding gene twg5l2 thereof, recombinant plasmids are constructed, high-efficiency expression of the lipase mutant TWG5LN87Y in pichia pastoris is realized, and the relative enzyme activity of the mutant is 299% of that of the wild type. The lipase mutant catalyzes vitamin A acetate and palmitic acid to generate vitamin A palmitate, the yield of the lipase mutant is 190% of that of a wild type, and the lipase mutant has important industrial value.

The application discloses a molecular marker of Tilletia foetida and application thereof. The molecular marker of Tilletia foetida in the application comprises at least one gene fragment in OG0008430, OG0008601, OG0008603 and OG0008610. The molecular marker in the application can distinguish and identify Tilletia foetida and its similar species from the genome level, can more comprehensively, accurately and effectively reflect the molecular differences between Tilletia foetida and its similar species, provides a new scheme and way for detection and identification of Tilletia foetida, and has important value and significance for quarantine and prevention of Tilletia foetida.

The present invention relates to a kind of lipase mutant and its application in preparing epoxide, specifically to the lipase mutant with significantly improved peroxidation activity derived from Indian tilletia (Tilletia indica), its encoding nucleic acid, recombinant expression vector and recombinant expression transformant containing the nucleic acid sequence, and using the recombinant lipase or recombinant expression transformant as catalyst to prepare peroxy acid, and then the application of peroxy acid and olefin reaction to generate epoxide. Compared with other methods (including chemical method or biological method etc.) for preparing α-epoxy pinane, application enzyme disclosed in the present invention and its technical method, there are the advantages such as mild reaction conditions, environmental friendliness, lower cost, simple and easy to operate, easy to amplify, and have good application prospect in the field of producing epoxy products with carbon-carbon double bond as substrate.