36 results about "Tilletia" patented technology

The invention discloses an agricultural sterilizing composite containing thifluzamide, containing a first active ingredient thifluzamide, and a second active ingredient selected from tebuconazole, difenoconazole, hexaconazole, propiconazole and cyproconazole, wherein the weight ratio of the first active ingredient to the second active ingredient is (12 : 1)-(1 : 12). The sterilizing composite is applied to controlling rice sheath blight disease, rice false smut, wheat sharp eyespot, wheat loose kernel smut and wheat Tilletia contraversa of cereal crops, and has reasonable ingredients, curing and protecting function, good sterilizing effect, less times of spraying insecticide and lower cost of spraying insecticide. In addition, the activity and the sterilizing effect of the sterilizing composite are not simple addition of the activity of the active ingredients, but remarkable synergism is achieved. The invention has good safety on plants, slows down the generation of resistance and meets the safety requirement on agrochemicals.

The invention belongs to the field of agricultural fungicide and particularly relates to an agricultural compound fungicide. The effective components of the fungicide are pyraclostrobin and fenpyrazamine which are compounded. The weight ratio of the two components is 1: 0.1-10, preferably, 1: 2-3. The prepared fungicide expands fungicide spectrum and has high activity on botrytis cinerea, rhizoctonia, puccinia, ustilago, tilletia, corticium, pyrenophora and other pathogenic fungi, and is remarkable in synergy which is not simple composition of activity of the components. The fungicide is strong in preventive treatment efficacy, long in effect lasting time, small in resistance risk and capable of reducing field usage of effective components, reducing production and using cost and reducing fungicide residue and environment pollution. The fungicide is favorable for overcoming and delaying production of bacteria drug resistance and is safe for crops due to effective components with different action mechanisms.

The invention provides a method for identifying teliospores of Tilletia controversa Kuhn and Tilletia foetida and relates to the technical field of cell biology. The method includes the steps of 1, making slides, to be specific, smearing the teliospores of Tilletia controversa Kuhn and Tilletia foetida, respectively, after drying, dropwise adding oil camera oil to a microscopic portion, and covering the smears with cover slips; 2, performing observation, to be specific, placing the made slides under a laser confocal scanning microscope, selecting a proper scanning mode and scanning parameters to perform scan-observation and photographing, with the scanning mode being an xyz scanning mode and the scanning parameters including excitation wavelength and emission wavelength, the excitation wavelength of Tilletia controversa Kuhn being 488nm, the emission wavelength of Tilletia controversa Kuhn being 510 to 550nm, the excitation wavelength of Tilletia foetida being 488nm, and the emission wavelength of Tilletia foetida being 510 to 550nm. The method enables quick identification of the teliospores of Tilletia controversa Kuhn and Tilletia foetida.

The invention discloses a sterilizing composition. The effective components are Sedaxane and captan, the mass ratio of the both is 50:1-1:50. The composition has obvious synergism and is effective totreat diseases caused by rhizoctonia solani, puccinia horiana, pyrenophora graminea, ustilago esculenta, Tilletia, Erysiphe, pyrenophora teres, Septoria musiva, and Ramularia; the composition has special effect to seed-borne disease. The composition can be applied to treat seeds and spray stems and leaves, the effect duration lasts for 4-6 weeks; the composition can be applied to the disease prevention and treatment of cereal type crops; particularly, it is applied to the seed treatment for preventing wheat ustilago esculenta, wheat tilletia, wheat basal rot, sphacelotheca reiliana, corn stalkrot, corn gall smut and rice bakanae disease.

The invention discloses a standard molecule for detecting tilletia walkeri castebury&carris;. The standard molecule is a plasmid which can be duplicated, and the plasmid contains at least one of a sequence A (Seq ID N0.1) and a sequence B (Seq ID N0.2); the sequence A is 99.65% homologous with a 2.3kb nucleotide sequence of the mitochondrial DNA of the tilletia walkeri castebury&carris; and the sequence B is 100% homologous with the DNA sequence of a ribosomal internal transcribed spacer of the tilletia walkeri castebury&carris;. The invention also discloses a preparation method and detection method of the standard molecule. The standard molecule constructed in the invention can serve as a positive control for detecting the molecule of the tilletia walkeri castebury&carris; instead of the DNA of the tilletia walkeri castebury&carris; is suitable for qualitative and quantitative PCR (polymerase chain reaction) detection of the tilletia walkeri castebury&carris; and is suitable for the inspection and quarantine department of the port, the agricultural production department, the plant protection department and the like.

The invention relates to antigens and a polyclonal antibody used for discriminating Tilletia controversa Kuehn and Tilletia foetida. A semiantigen used for preparing the polyclonal antibody for discriminating Tilletia controversa Kuehn and Tilletia foetida has an amino acid sequence as shown in SEQ ID No. 1. An immunogen used for preparing the polyclonal antibody for discriminating Tilletia controversa Kuehn is characterized in that the semiantigen mentioned above is reconstructed and coupled so as to obtain the immunogen, the immunogen is used to immunize domestic rabbits, domestic rabbits generating high-titer whole blood are screened and whole blood is acquired from a selected domestic rabbit and undergoes separating and purifying so as to obtain the polyclonal antibody. The antibody can specifically identify Tilletia controversa Kuehn without undergoing an antigen-antibody reaction with Tilletia foetida, so the polyclonal antibody can be used for discriminating Tilletia controversa Kuehn and Tilletia foetida difficult to distinguish in a field.

The present invention discloses a primer and probe for detecting wheat Tilletia indica, and method. The present primer and prove can specificly distinguish wheat Tilletia indica, shorten detection time and promote the detection sensitivity.

The invention discloses a bactericidal composition containing tetramycin and thifluzamide. Binary compounding is performed on the tetramycin and the thifluzamide of effective components of the bactericidal composition, and the remaining components are auxiliary components; the mass ratio of the tetramycin and the thifluzamide of the effective components of the bactericidal composition is (1-50):(50-1); the mass fraction ratio of the tetramycin to the thifluzamide of the effective components in the preparation is 1 percent to 80 percent, and the remaining components are the auxiliary components allowed to be used and accepted in pesticides. The bactericidal composition disclosed by the invention is missible oil, suspending agent, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules, microcapsules and water aqua in dosage form; the bactericidal composition is mainly used for controlling powdery mildew of wheat, strawberries, grapes, apples and cucumbers, meanwhile, is capable of effectively controlling fungi, such as Rhizoctonia, Puccinia, Tolyposporium, Tilletia, Corticium and Pyrenophora, and especially has a special effect on diseases, such as banded sclerotial blight and damping off, caused by Basidiomycetes fungi.

The invention discloses a fungicide composition containing metalaxyl and thifluzamide. The fungicide composition comprises the effective components, including metalaxyl and thifluzamide which are compounded, and the balance of auxiliary components, wherein the mass ratio of the effective components metalaxyl and thifluzamide in the fungicide composition is (1-50) to (50-1), and the mass part ratio of the effective components metalaxyl and thifluzamide in the preparation is 1%-80%, and the balance is auxiliary components required to be used and accepted in pesticides. The fungicide composition has the dosage forms of missible oil, suspension, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules, microcapsules and water aqua, is mainly used for preventing and controlling powdery mildew of wheat, strawberries, grapes, apples, and cucumbers, meanwhile can effectively prevent and control fungi such as rhizoctonia, puccinia, ustilago, tilletia, corticium, pyrenophora, and especially has special effects to banded sclerotial blight, damping off and the like due to basidiomycotina fungus.

The invention relates to a bactericidal composition containing cyflufenamid and thifluzamide. The bactericidal composition comprises active ingredients including the cyflufenamid and the thifluzamide in a binary compound way, and the balance of auxiliary components, wherein the mass ratio of the active ingredient cyflufenamid to the active ingredient thifluzamide in the pesticide composition is (1-50): (50-1); the preparation comprises the active ingredients including the cyflufenamid and the thifluzamide with the mass percent of 1-80%, and the balance of acceptable auxiliary components which are allowed to be used in pesticide. The pesticide composition can be prepared into different preparations such as missible oil, suspending agent, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules, microcapsules or aqueous solution, is mainly used for controlling the powdery mildew of wheat, strawberries, grapes, apples and cucumbers, is capable of effectively controlling fungi such as rhizoctonia, puccinia, ustilago, tilletia, corticium and pyrenophora, and especially has special effect for diseases such as banded sclerotial blight and damping off caused by basidiomycotina fungus.

The present invention discloses specific primer and probe for detecting Tilletia walkeri and method for detecting Tilletia walkeri using the primer and probe. The inventive method can shorten greatly the detection time and promote the sensitivity. Single teliospore can be detected. Two kinds of pathogen teliospore can be simultaneously detected in the invention so detection efficiency is promoted.

The invention relates to a method for detecting Tilletia sphaerococca (Wallr)Waldh and T.caries (TCT) in Gramineae grass seed Agrostis spp L. by adopting the PCR primer technique, belonging to the Gramineae grass seed Tilletia detection field. The method designs five sets of PCR primers of a common outer primer, a common inner primer, an outer primer and a special primer of T.sphaerococca, and a special primer of the TCT, wherein, the common inner primer is used for the shell type amplification of the Tilletia teliospore so that the quality monitoring of the amplification process is realized to avoid false negative; the special primer of the T.sphaerococca and the special primer of the T.caries are used for the hypha genome DNA double special PCR amplification; each outer primer and each special primer are used for detecting two teliospores of the double shell type PCR. The method has high detection speed and is reliable; and the whole detection process is finished within one working day.

The invention relates to a sterilizing composition containing sedaxane and fluopicolide. The active component of the sterilizing composition is formed by compounding the sedaxane and the fluopicolide,and the rest is auxiliary components. The mass ratio of the active components, namely the sedaxane to the fluopicolide, of the pesticide composition is (1-50):(50-1), the mass part ratio of the active components, namely the sedaxane to the fluopicolide, in the preparation is 1 percent to 80 percent, and the rest is auxiliary components which are allowed to use and accept in the pesticide. The dosage forms of the pesticide composition are missible oil, a suspending agent, and the pesticide composition is mainly used for preventing and treating powdery mildew on triticeae, strawberries, grapes,apples and cucumbers; and moreover, the pesticide composition can effectively prevent and control fungi such as rhizoctonia, puccinia person, ustilago, tilletia, corticium and pyrenophora, in particular has a special effect on diseases, such as rhizoctonia solani and damping off, caused by basidiomycetes.

The invention discloses a bactericidal composition, which is prepared from triticonazole and fludioxonil as effective components, and the mass ratio of triticonazole to fludioxonil is (50:1)-(1:50). The composition disclosed by the invention has obvious synergistic effects, is effective to diseases caused by fusarium, ustilago, tilletia, pyrenophora teres, septoria, rhizoctonia solani and the like, is mainly used for preventing and treating diseases of cereal crops, leguminous crops and fruit trees, has special effects on seed-borne diseases, has a lasting period of 4-6 weeks, and can be usedfor preventing and treating diseases of cereal crops and especially preventing and treating ustilago tritici, stinking smut, wheat stem rot, maize head smut, maize stem rot, maize gall smut and rice bakanae disease in seed treatment.

The invention relates to a method for detecting TilletiafuscaEII&EV and T.bromi(Brockm.)Brockm by using a double PCR amplification technique and belongs to the field of detection of tilletia of grass family. In the invention, six primers, namely a universal outer primer, a universal inner primer, an outer primer of T.fusca, a specific primer of T.fusca, an outer primer of T.bromi and a specific primer of T.bromi, are designed. The universal outer primer is used for nested amplification of a hypha genomic DNA or teliospore, the universal inner primer is used for the nested amplification of teliospore, and the universal outer primer and the universal outer primer are used for quality monitoring in an amplification process and detecting the quality of extracted hypha genomic DNA and selection and breakage of teliospore and can avoid false negative in a detection process. The outer primers and specific primers of T.fusca and T.bromi can be used for the double specific PCR amplification of the hypha genomic DNA of the two kinds of bacteria, and the respective outer primers and specific primers are used in double nested PCR specific amplification of DNA of teliospore.

The invention relates to tilletia foetida genetic transformation, and more specifically relates to an agrobacterium-mediated tilletia foetida transformation method. The method comprises the followingsteps: culturing TFL teliospore, when a germination rate is greater than or equal to 60%, collecting mycelia, and preparing 1*106/ml mycelia liquid; inoculating agrobacterium in a LB medium, performing oscillation culture for 2-3 d at the temperature of 28 DEG C, diluting the material to OD600 being 0.5 by using an IM medium, continuously culturing for 7-8 h, and using the IM medium for diluting the material to OD600 being 0.45-0.5; spreading a layer of aseptic glassine paper at the CM medium, performing isopyknic mixing of agrobacterium bacteria liquid and mycelia liquid and adding AS to 200[mu]mol/l, coating the material at the glassine paper, culturing the material in dark for 24 h at the temperature of 22 DEG C; transferring the glassine paper to a novel CM medium, applying one surface coated with the bacteria liquid to the medium, and culturing the material at the temperature of 16 DEG C until bacterium colony is grown. The method successfully transfers the exogenous gene to thetilletia foetida genome and realizes stable heredity.

The invention relates to a method for infecting wheat by inoculating tilletia foetida to roots, which is characterized by comprising the following steps: (1) culturing hypha of tilletia foetida: coating a 2% water agar culture medium with tilletia foetida teleutospore suspension with concentration of 1*10<5>/ml, culturing at 16 DEG C, and observing and confirming until promycelium grows, wherein 0.1-0.5ml of suspension is added to the culture dish of every 9cm diameter, and the promycelium is used for inoculation in the next step when the size of the promycelium is 1/2 the diameter of the teleutospore; (2) inoculating: placing sprouted wheat seeds to the culture medium in which the hypha grows, placing the culture dish to a 5 DEG C incubator, and simultaneously carrying out vernalization and infection for 30-45d; and (3) after vernalization is finished, planting wheat seedlings on sterile soil, and culturing in an artificial climate incubator with day temperature 23 DEG C and night temperature 21 DEG C, wherein the sprouted wheat seeds are sprouted wheat with coleoptile not more than 1mm. By adopting the method, wheat plants with roots infected by tilletia foetida and other parts presenting normal are obtained.

The invention relates to genetic transformation of Tilletia contraversa Kuhu, and specially relates to an agrobacterium tumefaciens-mediated transformation method for the Tilletia contraversa Kuhu. The method includes the following steps: culturing TCK teliospores, collecting mycelia when the germination rate is more than or equal to 60%, and preparing a mycelium liquid with 1*10<6>/ml; inoculating the agrobacterium tumefaciens in an LB culture medium, performing shake cultivation at 28 DEG C for 2-3 d, diluting with an IM culture medium until OD<600> is 0.15-0.35, continuing to culture for 7-8 h, and diluting with the IM medium to the OD<600> is 0.53-0.59; laying a layer of sterile cellophane on a CM medium, isovolumetrically mixing an agrobacterium tumefaciens liquid with the mycelium liquid, coating the cellophane with the mixture, and performing darkness culture at 20-22 DEG C for 48 h; and then transferring the cellophane to a new CM medium to make one side coated with a bacterialsolution attach to the culture medium, and performing the darkness culture at 5 DEG C until a bacterium colony grows. According to the method, foreign genes can be successfully transferred into a genome of the Tilletia contraversa Kuhu and can be stably inherited.