5 results about "Tilletia" patented technology

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The invention relates to tilletia foetida, in particular to a tilletia foetida effect protein TlCDA and application thereof. The invention provides an effect protein of tilletia foetida, and the amino acid sequence of the effect protein is shown as SEQ ID NO: 2. The effector protein can effectively inhibit programmed death of plant cells induced by BAX, and plays an important role in inhibiting a plant defense reaction process. The invention lays a foundation for deeply researching the pathogenesis of the tilletia foetida, excavating the tilletia foetida-resistant gene of the wheat and developing the tilletia foetida-resistant wheat variety.

The application discloses a wheat Tilletia indica-specific biomarker combination, a screening method and application thereof, and the biomarker combination comprises 20-hydroxyarachidonic acid, 1-octadecyl lysophosphatidic acid, linolenic acid, 13-hydroxydocosahexaenoic acid, 4-methoxy estrone, hypoxanthine, 3-hydroxymyristic acid, beta-ecdysterone, 17-phenyltrinorprostaglandin F2 alpha cyclopropylmethylamide, 11,12-dihydroxyeicosatrienoic acid and oxidized octadecadienoic acid. When the biomarker combination is used for identifying Tilletia indica, the time-consuming long and the accuracy easily interfered by the spore development stage limitations of traditional morphological identification are overcome; in view of the molecular level identification difficulty caused by the high genome homology of Tilletia indica and Tilletia controversa, the application digs the feature difference at the level of metabolomics, and constructs a specific chemical fingerprint, so that the technical bottleneck that the close relatives are difficult to accurately distinguish is effectively solved.

The invention belongs to the technical field of gene engineering of enzymes, and particularly relates to lipase as well as preparation and application thereof. The wild type lipase TWG5L coding gene from Tilletia walkeri is obtained through whole gene synthesis, and the wild type lipase and the coding gene twg5l of the wild type lipase are obtained. A wild type lipase gene is modified by using a key site amino acid residue site-directed mutagenesis technology to obtain a lipase mutant TWG5LN87Y and an encoding gene twg5l2 thereof, recombinant plasmids are constructed, high-efficiency expression of the lipase mutant TWG5LN87Y in pichia pastoris is realized, and the relative enzyme activity of the mutant is 299% of that of the wild type. The lipase mutant catalyzes vitamin A acetate and palmitic acid to generate vitamin A palmitate, the yield of the lipase mutant is 190% of that of a wild type, and the lipase mutant has important industrial value.

The application discloses a molecular marker of Tilletia foetida and application thereof. The molecular marker of Tilletia foetida in the application comprises at least one gene fragment in OG0008430, OG0008601, OG0008603 and OG0008610. The molecular marker in the application can distinguish and identify Tilletia foetida and its similar species from the genome level, can more comprehensively, accurately and effectively reflect the molecular differences between Tilletia foetida and its similar species, provides a new scheme and way for detection and identification of Tilletia foetida, and has important value and significance for quarantine and prevention of Tilletia foetida.