The invention provides an application of 10E, 12Z-
octadecadienoic acid in inhibition of inflammatory reactions, and the inflammatory reactions comprise macrophage inflammatory reactions induced by
lipopolysaccharide. According to the invention, a mouse mononuclear macrophage RAW264.7
cell inflammatory response model is constructed. The method comprises the following steps: firstly, measuring CC50 of 10E, 12Z-
octadecadienoic acid by a CCK-8 method; 10E, 12Z-
octadecadienoic acid is used for pretreating macrophages for 24 hours before
lipopolysaccharide stimulation at the final concentration of 25 mu mol / L to 100 mu mol / L, qPCR (quantitative
polymerase chain reaction) and ELISA (
enzyme-linked immuno
sorbent assay) experiments prove that the 10E, 12Z-octadecadienoic acid plays an anti-inflammatory role by inhibiting mRNA (
messenger ribonucleic acid) expression and
protein secretion of IL-6, IL-1beta and / or TNF-alpha, and the anti-inflammatory effect is optimal at the final concentration of 100 mu mol / L. The 10E, 12Z-octadecadienoic acid is a single unmodified
fatty acid isomer, is clear in structure, good in anti-inflammatory effect
repeatability and good in
biocompatibility, and can reduce adverse reactions compared with traditional anti-inflammatory drugs; compared with existing
fatty acid anti-inflammatory components, the composition is clear in component and simple and convenient to prepare. The application scenarios of the invention cover the development of anti-inflammatory drugs, functional foods and biological agents.