68 results about "Cadaverine" patented technology

The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

The invention provides a method for trapping and collecting wild hermetia illucens by utilizing chrysomyia megacephala maggot cultivation residues. Specifically, bran and spray-dried animal blood cells are utilized to breed chrysomyia megacephala maggots firstly, then the chrysomyia megacephala maggot cultivation residues are utilized to trap and collect the wild hermetia illucens. According to the method, the suitable period for trapping and collecting the wild hermetia illucens ranges from April to October at the air temperature from 23 DEG C to 35 DEG C on clear and rainless days. The method is quite obvious in the effects of trapping and collecting the hermetia illucens, especially the lysine richly contained in the spray-dried animal blood cells is easily broken into cadaverine and other substances with strong stimulating smell under the action of the chrysomyia megacephala maggots and natural microorganism, the cadaverine and other substances with strong stimulating smell have an extremely strong effect of inducing adult hermetia illucens to lay eggs, and after the eggs of the wild hermetia illucens are hatched and grow under normal temperature, a great quantity of hermetia illucen larva resources can be obtained in a short time. The method is simple, convenient to operate, effective and capable of greatly saving manpower and time for obtaining the wild hermetia illucen larva resources.

To improve problems of the content of trifunctional and higher organic compounds, fish-eyes of a film and its flowability in injection molding occurring in the existing techniques, the total content of trifunctional and higher organic compounds of cadaverine salts is regulated to 90 ppm or less. To improve the fish-eyes (F/E) of a polyamide resin film and its flowability in injection molding and the amount of high-molecule impurities contained in the polyamide resin, an aqueous cadaverine salt solution, from which high-molecule impurities having a molecular weight of 12,000 or more have been removed, is prepared. To easily and economically produce a cadaverine solution by which defects in the surface appearance such as fish-eyes can be prevented in the case of using it as a starting material of a polyamide resin film or the like, moreover, the amount of microbial cells employed in the reaction is regulated within a definite range.

The invention provides a method for rapidly detecting multiple biogenic amines in a dairy product by virtue of combination of high performance thin layer chromatography and adjustable surface enhanced raman spectroscopy, and belongs to the technical field of food detection. The method comprises the steps of preparation of nano silver colloid, preparation of samples, separation based on high performance thin layer chromatography, HPTLC-SERS detection and the like. The HPTLC-SERS detection method for identifying structures of the multiple biogenic amines (tyramine, putrescine and cadaverine) is established, and has the advantages of economical efficiency, rapidness, simplicity and convenience; furthermore, by virtue of the selectivity enhancement function of a new synergist, the application range of HPTLC-SERS detection is expanded, and a new visual field is opened for directly and rapidly detecting a chromatoplate for chemical derivatization.

The present invention relates to a novel customized sRNA that reduces gene expression in prokaryotic cells, a preparation method thereof, and the use thereof, and more particularly to a synthetic sRNA comprising an Hfq binding site, derived from the sRNA of any one of MicC, SgrS and MicF, and a region that base-pairs with the target gene mRNA, and to a preparation method thereof and the use thereof. The synthetic sRNA according to the invention has an advantage in that the degree of inhibition of the target gene can be controlled by regulating the ability of the synthetic sRNA to bind to the mRNA of the target gene. The use of the synthetic sRNA that regulates the expression of the target gene makes it possible to effectively construct a recombinant microorganism without using a conventional gene deletion method and to reduce the expression of the target gene, and thus the synthetic sRNA is useful for the production of recombinant microorganisms. Also, the synthetic sRNA can be quickly applied to various strains, and thus is very suitable for the measurement of metabolic capabilities of strains and the selection of the most suitable strain. In addition, recombinant microorganisms, which are obtained by metabolic flux manipulation using the synthetic sRNA and produce tyrosine or cadaverine with high efficiency, are useful in the drug and industrial fields. In other words, the use of the sRNA according to the present invention can make it easy to select target genes whose expression is to be inhibited for the highly efficient production of metabolites. Accordingly, the synthetic sRNA can be used to construct recombinant strains for efficient production of various metabolites and to establish efficient methods for production of various metabolites, and thus is highly useful.

Disclosed are diagnostic tests helpful in indicating presence of an inflammatory bowel disease (IBD) in a human patient. In one embodiment, the test comprises obtaining a sample of mucosal tissue from the ileum or sigmoid colon of the patient; evaluating sample quality by testing for cadaverine and continuing the diagnostic test if the sample has no detectable cadaverine; testing the cadaverine negative sample for N-acetylated spermine; and correlating a detectable level of N-acetylated spermine in the sample with presence of IBD in the patient. Another, less invasive, method disclosed includes a diagnostic test comprising isolating mononuclear leukocytes from the patient's blood; testing the isolated mononuclear leukocytes for level of spermidine; and correlating a level of spermidine higher than that in mononuclear leukocytes of normal subjects as indicative of an inflammatory bowel disease in the patient.

The invention discloses a novel MCO (multicopper oxidase) with the function of efficient degradation of histamine, and belongs to the technical field of molecular biology. The sequence of the MCO is shown as SEQ ID NO.1. The enzyme has higher histamine degradation performance, is wide in degradation spectrum, can degrade tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine andspermidine, particularly has good degradation effect on histamine, has histamine degradation rate of 82% within 24 h and has important industrial application potential.

The invention discloses lactobacillus plantarum concurrently having biogenic amine degradation activity and biological acid reduction activity, and an application of the lactobacillus plantarum to fruit wine. The strain is lactobacillus plantarum, is separated from fruit peel, is named as Lactobacillus plantarum ZZ46, and is abbreviated as ZZ46, and the preservation number is CGMCC No.19241. The obtained lactobacillus plantarum ZZ46 disclosed by the invention can efficiently degrade histamine, tyramine and cadaverine in the fruit wine, is well adapted to wine body environment being high in alcoholic strength, low in pH value and high in SO2, can rapidly degrade malic acid in the fruit wine and is high in safety. According to the lactobacillus plantarum disclosed by the invention, technicalsupport is provided for production of high-quality safe fruit wine products in fruit production areas in China. The obtained strain is subjected to multiple steps of biogenic amine degradation activity screening, biological acid reduction activity screening, tolerance screening and the like, and a screening method and route are provided for obtaining strains having the same functions.

The invention provides a method for trapping wild Hermetia illucens species. The method comprises the steps of breeding lucilia sericata maggots through bran and spray-dried blood cells, and then trapping the wild Hermetia illucens species in a wild cool and ventilated area. By means of the method, the effect of trapping the wild Hermetia illucens species is quite significant; especially, rich lysine in the spray-dried blood cells can easily decompose into substances with strong stimulating smells like cadaverine under the effect of the lucilia sericata maggots and natural microorganisms and has a strong effect of trapping wild Hermetia illucens adults into ovipositing; after wild Hermetia illucens eggs are hatched and grow under a normal temperature, a large number of Hermetia illucens species can be obtained within a short time. The method is simple, convenient to implement, effective and capable of saving a great deal of labor and time for obtaining the wild Hermetia illucens species.

An evaluating method includes an evaluating step of evaluating a state of colorectal cancer for a subject to be evaluated using (i) a concentration value of at least one metabolite of bABA, N-Me-bABA, 1-Me-His, aABA, Aminoadipic acid, bAiBA, Cadaverine, Ethylglycine, GABA, Homoarginine, Hypotaurine, Kinurenine, N6-Acetyl-L-Lys, Putrescine, Serotonin, Spermidine, Spermine, ADMA, Homocitrulline, 3-Me-His, Hydroxyproline, Phosphoethanolamine, SDMA, Acylcarnitine (13:1), and EPA in blood of the subject or (ii) a value of a formula calculated using the concentration value of the metabolite and the formula including an explanatory variable to be substituted with the concentration value of the metabolite.

The invention relates to a pentiptycene derivative represented by formula I, n is 1 or 3, and R is H or OMe. The invention also relates to a preparation method of the pentiptycene derivative and a preparation method, application thereof in polyamine compound detection, a fluorescent sensor comprising the pentiptycene derivative represented by the formula I and a method for detecting polyamine compounds. The polyamine compounds are particularly putrescine, cadaverine, spermidine and spermine. The pentiptycene derivative shown in the formula I does not emit light basically in a free state, and emits fluorescent light in the presence of the polyamine compounds. The pentiptycene derivative has an aromatic group comprising a donor-pi-receptor manner and a fluorescent group having an aggregation-induced luminescence effect, so that the fluorescence intensity is very obvious. Therefore, the pentiptycene derivative represented by the formula I can be used as a fluorescent probe of the polyamine compounds, especially can be used for detecting biological polyamine compounds in an animal and a plant body, and can be used as an effective means for researching a biological model and detecting life metabolites.

The invention provides an AuNPs-p-Ti3C2Tx composite material as well as a preparation method and application thereof, and belongs to the technical field of composite material preparation, sensor preparation and meat product quality detection. According to the invention, the AuNPs-p-Ti3C2Tx composite material is firstly prepared, then the bimolecular imprinting sensor is prepared based on the composite material, the bimolecular imprinting sensor is easy and convenient to operate, rapid and sensitive, excellent in selectivity, high in stability and extremely low in detection limit, the sum of the content of cadaverine and the content of putrescine can be detected at the same time, and the bimolecular imprinting sensor has good application in evaluation of pork freshness.

The invention discloses a three-dimensional flexible SERS (Surface Enhanced Raman Scattering) substrate and a putrescine and cadaverine detection method based on the substrate. The substrate is prepared by the following method: 1) preparing clustered ZnO nanorods on a PVDF (Polyvinylidene Fluoride) film; 2) coating the ZnO nanorod with a gold film; 3) after modification by polyethyleneimine, fixing gold nanoparticles on the surface of the ZnO nanorod; and (4) Au < 3 + > is reduced, AuNPs further grows, and the three-dimensional flexible SERS substrate is obtained. According to the preparation method, the 3D rose dendritic nanostructure is prepared through in-situ chemical growth of AuNPs on the surface of the ZnO nanorod, and the SERS activity of the heterostructure is remarkably enhanced through the LSPR effect and the CT effect; the p-MBA modified 3D rose dendritic SERS substrate can realize high detection sensitivity and rapid detection of putrescine and cadaverine in a sample, and has a good application prospect in instant detection of meat products.