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68 results about "Cadaverine" patented technology
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Cadaverine is a foul-smelling diamine compound produced by the putrefaction of animal tissue. Cadaverine is a toxic diamine with the formula NH₂(CH₂)₅NH₂, which is similar to putrescine's NH₂(CH₂)₄NH₂. Cadaverine is also known by the names 1,5-pentanediamine and pentamethylenediamine.
Gene products of bacillus licheniformis which form odorous substances and improved biotechnological production methods based thereon
InactiveUS20070190605A1Reduce formationImprove filtering effectBacteriaHydrolasesBacillus licheniformisPropanoic acid
The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and / or isobutyric acid (as part of the catabolism of valine and / or isoleucine), 3) butanol and / or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and / or 5) cadaverine and / or putrescine (as parts of the catabolism of lysine and / or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.
Owner:BASF AG
Method for producing cadaverine dicarboxylate
Owner:AJINOMOTO CO INC
Novel synthesis-regulating srna and method for preparing same
Owner:KOREA ADVANCED INST OF SCI & TECH
Method for producing cadaverine dicarboxylate
ActiveUS20050003497A1Produced economicallyAdequate levelBacteriaFermentationCadaverineDicarboxylic acid
Cadaverine dicarboxylate is produced by performing an enzymatic decarboxylation reaction of a lysine solution while adding a dicarboxylic acid containing 4 to 10 carbons to the lysine solution to maintain pH of the solution at a level sufficient for the enzymatic decarboxylation reaction to occur, for example, 4.0 to 8.0.
Owner:AJINOMOTO CO INC
Method for trapping and collecting wild hermetia illucens by utilizing chrysomyia megacephala maggot cultivation residues
InactiveCN103975901ASignificant trappingThe effect of strong oviposition inductionFood processingAnimal feeding stuffMicroorganismAnimal science
The invention provides a method for trapping and collecting wild hermetia illucens by utilizing chrysomyia megacephala maggot cultivation residues. Specifically, bran and spray-dried animal blood cells are utilized to breed chrysomyia megacephala maggots firstly, then the chrysomyia megacephala maggot cultivation residues are utilized to trap and collect the wild hermetia illucens. According to the method, the suitable period for trapping and collecting the wild hermetia illucens ranges from April to October at the air temperature from 23 DEG C to 35 DEG C on clear and rainless days. The method is quite obvious in the effects of trapping and collecting the hermetia illucens, especially the lysine richly contained in the spray-dried animal blood cells is easily broken into cadaverine and other substances with strong stimulating smell under the action of the chrysomyia megacephala maggots and natural microorganism, the cadaverine and other substances with strong stimulating smell have an extremely strong effect of inducing adult hermetia illucens to lay eggs, and after the eggs of the wild hermetia illucens are hatched and grow under normal temperature, a great quantity of hermetia illucen larva resources can be obtained in a short time. The method is simple, convenient to operate, effective and capable of greatly saving manpower and time for obtaining the wild hermetia illucen larva resources.
Owner:SUN YAT SEN UNIV
Cadaverine salt, aqueous cadaverine salt solution, polyamide resin, molded article and process for producing cadaverine salt and aqueous cadaverine salt solution
ActiveCN101578256AExcellent surface appearancePrevent surface appearance defectsAmino compound purification/separationRecombinant DNA-technologyCadaverineOrganic compound
To improve problems of the content of trifunctional and higher organic compounds, fish-eyes of a film and its flowability in injection molding occurring in the existing techniques, the total content of trifunctional and higher organic compounds of cadaverine salts is regulated to 90 ppm or less. To improve the fish-eyes (F / E) of a polyamide resin film and its flowability in injection molding and the amount of high-molecule impurities contained in the polyamide resin, an aqueous cadaverine salt solution, from which high-molecule impurities having a molecular weight of 12,000 or more have been removed, is prepared. To easily and economically produce a cadaverine solution by which defects in the surface appearance such as fish-eyes can be prevented in the case of using it as a starting material of a polyamide resin film or the like, moreover, the amount of microbial cells employed in the reaction is regulated within a definite range.
Owner:MITSUBISHI RAYON CO LTD
Method for attracting wild hermetia illucens
InactiveCN103975902AThe effect of strong oviposition inductionPromote decompositionAnimal feeding stuffMicroorganismAnimal science
The invention provides a method for attracting wild hermetia illucens. The method includes the following specific steps of breeding housefly larvae, and then attracting the wild hermetia illucens outdoors by using residual breeding materials on clear and rainless days at the temperature of 23 DEG C to 35 DEG C from April to October. By means of the method, the effect of attracting the hermetia illucens is very significant, in particular, the composts used for breeding the housefly larvae are rich in lysine which can be extremely easily decomposed into cadaverine and other substances with pungent smells under the action of the housefly larvae and natural microbes, and cadaverine and other substances with pungent smells have an extremely strong attracting effect on the wild hermetia illucens adults. After the egg-laying and development of the hermetia illucens are finished, a large number of hermetia illucens provenances can be obtained easily in a short time. The method is simple, convenient to conduct and efficient, and can greatly save labor and time for obtaining the wild hermetia illucens provenances.
Owner:SUN YAT SEN UNIV
Method for rapidly detecting multiple biogenic amines in dairy product by virtue of combination of high performance thin layer chromatography and adjustable surface enhanced raman spectroscopy
ActiveCN106706834ASuppress masking effectWell-characterized Raman fingerprintsComponent separationVisual field lossSurface-enhanced Raman spectroscopy
The invention provides a method for rapidly detecting multiple biogenic amines in a dairy product by virtue of combination of high performance thin layer chromatography and adjustable surface enhanced raman spectroscopy, and belongs to the technical field of food detection. The method comprises the steps of preparation of nano silver colloid, preparation of samples, separation based on high performance thin layer chromatography, HPTLC-SERS detection and the like. The HPTLC-SERS detection method for identifying structures of the multiple biogenic amines (tyramine, putrescine and cadaverine) is established, and has the advantages of economical efficiency, rapidness, simplicity and convenience; furthermore, by virtue of the selectivity enhancement function of a new synergist, the application range of HPTLC-SERS detection is expanded, and a new visual field is opened for directly and rapidly detecting a chromatoplate for chemical derivatization.
Owner:JIANGNAN UNIV
Method for predicting or identifying the onset of premature membrane rupture during pregnancy
A diagnostic test kit for simultaneously detecting the presence of amines (e.g., putrescine, cadaverine, tyramine, and trimethylamine) and C-reactive protein in a vaginal sample is provided. The test kit includes a lateral flow assay device containing a chromatographic medium (e.g., porous membrane, fluidic channel, etc.). The chromatographic medium defines a first detection zone within which is contained an amine-sensitive chromogen and a second detection zone within which is contained an immunoreactive receptive material that preferentially binds with C-reactive protein or a specific binding member thereof. The first and second detection zones may produce signals that are detectable, either visually or through the use of instrumentation. In this manner, the kit provides a complimentary system for both predicting and identifying the onset of premature membrane rupture. For example, the detection of amines in the vaginal sample may serve as a diagnosis of bacterial vaginosis, which may provide an early warning of the potential for premature membrane rupture. The complimentary detection of CRP may serve as an indicator of rupture and thus alert the patient to seek immediate medical care. Such a system may be equally effective in point of care (POC) and over-the-counter (OTC) applications.
Owner:KIMBERLY-CLARK WORLDWIDE INC
Novel synthesis-regulating srna and method for preparing same
ActiveUS20140377752A1Improve efficiencyOvercome limitationsBacteriaSugar derivativesMetaboliteBinding site
The present invention relates to a novel customized sRNA that reduces gene expression in prokaryotic cells, a preparation method thereof, and the use thereof, and more particularly to a synthetic sRNA comprising an Hfq binding site, derived from the sRNA of any one of MicC, SgrS and MicF, and a region that base-pairs with the target gene mRNA, and to a preparation method thereof and the use thereof. The synthetic sRNA according to the invention has an advantage in that the degree of inhibition of the target gene can be controlled by regulating the ability of the synthetic sRNA to bind to the mRNA of the target gene. The use of the synthetic sRNA that regulates the expression of the target gene makes it possible to effectively construct a recombinant microorganism without using a conventional gene deletion method and to reduce the expression of the target gene, and thus the synthetic sRNA is useful for the production of recombinant microorganisms. Also, the synthetic sRNA can be quickly applied to various strains, and thus is very suitable for the measurement of metabolic capabilities of strains and the selection of the most suitable strain. In addition, recombinant microorganisms, which are obtained by metabolic flux manipulation using the synthetic sRNA and produce tyrosine or cadaverine with high efficiency, are useful in the drug and industrial fields. In other words, the use of the sRNA according to the present invention can make it easy to select target genes whose expression is to be inhibited for the highly efficient production of metabolites. Accordingly, the synthetic sRNA can be used to construct recombinant strains for efficient production of various metabolites and to establish efficient methods for production of various metabolites, and thus is highly useful.
Owner:KOREA ADVANCED INST OF SCI & TECH
Diagnostic tests for inflammatory bowel diseases
InactiveUS7972807B1Improved diagnostic testingMicrobiological testing/measurementDisease diagnosisWhite blood cellCadaverine
Disclosed are diagnostic tests helpful in indicating presence of an inflammatory bowel disease (IBD) in a human patient. In one embodiment, the test comprises obtaining a sample of mucosal tissue from the ileum or sigmoid colon of the patient; evaluating sample quality by testing for cadaverine and continuing the diagnostic test if the sample has no detectable cadaverine; testing the cadaverine negative sample for N-acetylated spermine; and correlating a detectable level of N-acetylated spermine in the sample with presence of IBD in the patient. Another, less invasive, method disclosed includes a diagnostic test comprising isolating mononuclear leukocytes from the patient's blood; testing the isolated mononuclear leukocytes for level of spermidine; and correlating a level of spermidine higher than that in mononuclear leukocytes of normal subjects as indicative of an inflammatory bowel disease in the patient.
Owner:UNIV OF CENT FLORIDA RES FOUND INC
Method of preparing cadaverine by virtue of immobilized hafnia alvei
InactiveCN103725724AImprove stabilityImprove productivityMicroorganism based processesFermentationProduction rateHigh concentration
The invention relates to a method of preparing cadaverine by virtue of immobilized hafnia alvei (Hafnia Alvei) AS1.1009, belongs to the field of microbial fermentation and aims to increase the production rate of the cadaverine by utilizing the microbial embedding technology. The method comprises the steps: (1) carrying out third-stage culturing on a hafnia alvei strain; (2) embedding and fixing a bacteria solution by utilizing an embedding carrier under the aseptic condition; and (3) adding an immobilized thallus into a fermentation tank, and carrying out fermentation conversion to prepare the cadaverine, wherein the conversion rate of the cadaverine after the fermentation reaches 95.31-97.42 percent. With the adoption of the embedded high-concentration hafnia alvei, a strain microorganism with high concentration is provided; and the stability of the strain and the tolerance of the concentration of a substrate are improved by embedding microorganism particles, the enzymatic activity is improved, and the productivity of the cadaverine is increased.
Owner:天津北洋百川生物技术有限公司
Novel MCO (multicopper oxidase) with function of efficient degradation of histamine
InactiveCN109468288APromote degradationStrong performance in degrading biogenic aminesBacteriaMicroorganism based processesTryptamineCadaverine
Owner:GUANGDONG PRB BIO TECH CO LTD +1
Seafood spoilage indicator
InactiveUS7560271B2Bioreactor/fermenter combinationsBiological substance pretreatmentsCadaverineColor changes
An enzyme based nondestructive sensor for the qualitative detection of spoilage in seafood is provided wherein the sensor does not alter the physical composition of the seafood specimen. The sensor comprises a sampling matrix, at least three or more enzymes in contact with the sampling matrix, and at least one indicator compound in contact with the sampling matrix. The enzymes are capable of interacting with four target chemicals comprising putrescine, cadaverine, histamine and tyramine, which are located on the surface of the seafood specimen. The indicator compound is capable of changing the color of the sampling matrix thereby indicating a qualitative visually detectable color change. A method for the nondestructive detection of the quality of a seafood specimen at any given time and for determining the remaining usable shelf life of the seafood specimen is disclosed.
Owner:FLIR DETECTION
Apparatus for continuous production and extraction of cadaverine via immobilized cells
ActiveCN106367326ASolve the problem of extraction in continuous productionIncrease profitBioreactor/fermenter combinationsAmino compound purification/separationElutionCadaverine
The invention discloses an apparatus for continuous production and extraction of cadaverine via immobilized cells. The apparatus comprises a feed conveying part, a cadaverine converting part, a pH value adjusting part, a column adsorption and elution part and a centrifugal extraction part, wherein a feed tank, an integrated cadaverine conversion tank and a calcium phytate column are in circulation connection via pipelines; a pH value adjusting liquid tank is connected on a pipeline located between the feed tank and integrated cadaverine conversion tank via a pipeline; an eluate storage tank is connected with the calcium phytate column via a pipeline; and a centrifugal extraction machine is connected with the calcium phytate column. Compared with the prior art, the apparatus for continuous production and extraction of cadaverine via the immobilized cells perfectly overcome problems in continuous production and extraction of cadaverine, is applicable to large-scale production and operation of cadaverine, and has the characteristics of simple operation, high equipment utilization rate, stable product quality, sufficient utilization of raw materials, easiness in promotion, etc.
Owner:NANJING UNIV OF TECH
Method for breeding Boettcherisca peregrine maggots and trapping wild black soldier flies
InactiveCN104026079AThe effect of strong oviposition inductionPromote decompositionFood processingAnimal feeding stuffMicroorganismMaggot
The invention provides a method for breeding Boettcherisca peregrine maggots and trapping wild black soldier flies. The method specifically comprises the steps of breeding the Boettcherisca peregrine maggots by utilizing bran and albuminoidal substances rich in lysine and then trapping the wild black soldier flies by utilizing breeding remaining materials. The effect of trapping the wild black soldier flies is quite obvious, especially the rich lysine in the breeding materials is easily decomposed into substances with strong stimulating smells like pentamethylene diamine under the action of the Boettcherisca peregrine maggots and natural microorganisms, the substances with the strong stimulating smells have the strong effect on inducing wild black soldier fly adults to spawn, wild black soldier fly eggs are hatched and grow under the constant temperature, and therefore a large number of black soldier fly provenances can be obtained in the short time. The method is simple, convenient, effective and capable of greatly saving manual work and time of obtaining the black soldier fly provenances.
Owner:SUN YAT SEN UNIV
Cadaverine salt, aqueous cadaverine salt solution, polyamide resin, molded article and process for producing cadaverine salt and aqueous cadaverine salt solution
ActiveCN101578256BExcellent surface appearancePrevent surface appearance defectsAmino compound purification/separationRecombinant DNA-technologyCadaverineOrganic compound
Owner:MITSUBISHI RAYON CO LTD
Lactobacillus plantarum concurrently having biogenic amine degradation activity and biological acid reduction activity, and application of lactobacillus plantarum to fruit wine
ActiveCN111363699AHigh alcohol contentImprove qualityBacteriaMicroorganism based processesBiotechnologyReduction Activity
The invention discloses lactobacillus plantarum concurrently having biogenic amine degradation activity and biological acid reduction activity, and an application of the lactobacillus plantarum to fruit wine. The strain is lactobacillus plantarum, is separated from fruit peel, is named as Lactobacillus plantarum ZZ46, and is abbreviated as ZZ46, and the preservation number is CGMCC No.19241. The obtained lactobacillus plantarum ZZ46 disclosed by the invention can efficiently degrade histamine, tyramine and cadaverine in the fruit wine, is well adapted to wine body environment being high in alcoholic strength, low in pH value and high in SO2, can rapidly degrade malic acid in the fruit wine and is high in safety. According to the lactobacillus plantarum disclosed by the invention, technicalsupport is provided for production of high-quality safe fruit wine products in fruit production areas in China. The obtained strain is subjected to multiple steps of biogenic amine degradation activity screening, biological acid reduction activity screening, tolerance screening and the like, and a screening method and route are provided for obtaining strains having the same functions.
Owner:LUDONG UNIVERSITY
Method for improving yield of cadaverine produced by utilizing microorganisms for saccharides fermentation
InactiveCN105441496AIncrease profitIncrease productionMicroorganism based processesPeptidesBiotechnologyMicroorganism
The invention relates to a method for improving yield of cadaverine produced by utilizing microorganisms for saccharides fermentation. According to the method, key regulation and control sites in a metabolic pathway for synthesizing cadaverine through microorganisms are modified, and preferably, the key regulation and control sites are lysine transport protein gene lysE site and / or D-glucose-6-phosphateketol-isomerase gene pgi site, or the key regulation and control sites are regulation and control sites for regulating and controlling expression of the above genes. The modification method for the regulation and control sites comprises deleting lysine transport protein gene lysE and / or D-glucose-6-phosphateketol-isomerase gene pgi, or weakening expression or enzyme activity of lysine transport protein gene lysE and / or D-glucose-6-phosphateketol-isomerase gene pgi, and therefore the yield of cadaverine is effectively improved, saccharides utilization rate is increased, and production cost is reduced.
Owner:TIANJIN UNIV OF SCI & TECH
Production Process for Fine Chemicals Using Microorganisms with Reduced Isocitrate Dehydrogenase Activity
The present invention is directed to a method utilizing a microorganism with reduced isocitrate dehydrogenase activity for the production of fine chemicals. Said fine chemicals may be amino acids, monomers for polymer synthesis, sugars, lipids, oils, fatty acids or vitamins and are preferably amino acids of the aspartate family, especially methionine or lysine, or derivatives of said amino acids, especially cadaverine. Furthermore, the present invention relates to a recombinant microorganism having a reduced isocitrate dehydrogenase activity in comparison to the initial microorganism and the use of such microorganisms in producing fine chemicals such as aspartate family amino acids and their derivatives.
Owner:BASF AG
Method for trapping wild Hermetia illucens species
InactiveCN104026080AThe effect of trapping wild black soldier flies is remarkablePromote decompositionFood processingAnimal feeding stuffMicroorganismAnimal science
The invention provides a method for trapping wild Hermetia illucens species. The method comprises the steps of breeding lucilia sericata maggots through bran and spray-dried blood cells, and then trapping the wild Hermetia illucens species in a wild cool and ventilated area. By means of the method, the effect of trapping the wild Hermetia illucens species is quite significant; especially, rich lysine in the spray-dried blood cells can easily decompose into substances with strong stimulating smells like cadaverine under the effect of the lucilia sericata maggots and natural microorganisms and has a strong effect of trapping wild Hermetia illucens adults into ovipositing; after wild Hermetia illucens eggs are hatched and grow under a normal temperature, a large number of Hermetia illucens species can be obtained within a short time. The method is simple, convenient to implement, effective and capable of saving a great deal of labor and time for obtaining the wild Hermetia illucens species.
Owner:SUN YAT SEN UNIV
Preparation of cadaverine
The invention relates to a method for preparing cadaverine comprising: (1) obtaining an aqueous cadaverine salt composition from a fermentation system; (2) adjusting the pH of the aqueous cadaverine salt composition to a pH of at least about 12 by adding an inorganic base composition to provide a first composition comprising an aqueous cadaverine composition having a pH of at least about 12 and a solid composition comprising one or more alkali metal sulfates; (3) subjecting the first composition to solid-liquid separation without organic solvent extraction to provide the aqueous cadaverine composition and the solid composition; (4) distilling or evaporating the aqueous cadaverine composition of step (3) without organic solvent extraction to provide purified cadaverine; (5) preparing a saturated solution of the alkali metal sulfate(s) from the solid composition of step (3) at a temperature of about 35-40° C., mixing the saturated solution with NH4HCO3, and subjecting the mixture to solid-liquid separation to provide Solution (I) and Solid (I); (6) adjusting the temperature of Solution (I) to about 60-80° C., and obtaining Solution (II) and Solid (II) after solid-liquid separation; (7) concentrating Solution (II) and obtaining Solution (III) and Solid (III) after solid-liquid separation; and (8) adding Solid (III) back to the fermentation system of step (1); wherein steps (1) to (8) are optionally repeated one or more times.
Owner:CATHAY BIOTECH INC +1
Purification of cadaverine using high boiling point solvent
The present invention relates to a method for the purification of cadaverine from an aqueous cadaverine salt composition comprising one or more involatile impurities, the method comprising: (1) mixing the aqueous cadaverine salt composition with one or more bases selected from the group consisting of Ca(OH)2, Mg(OH)2, CaO and MgO to provide an aqueous cadaverine composition; and (2) distilling or evaporating the aqueous cadaverine composition to produce purified cadaverine; wherein one or more solvents are added to the evaporation / distillation system before the evaporation / distillation stops, and the one or more solvents comprise at least one or more high boiling point (HBP) solvents.
Owner:CIBT AMERICA INC +1
Purification of cadaverine using high boiling point solvent
The present invention relates to a method for the purification of cadaverine from an aqueous cadaverine salt composition comprising one or more involatile impurities, the method comprising: (1) mixing the aqueous cadaverine salt composition with one or more bases selected from the group consisting of Ca(OH)2, Mg(OH)2, CaO and MgO to provide an aqueous cadaverine composition; and (2) distilling or evaporating the aqueous cadaverine composition to produce purified cadaverine; wherein one or more solvents are added to the evaporation / distillation system before the evaporation / distillation stops, and the one or more solvents comprise at least one or more high boiling point (HBP) solvents.
Owner:CIBT AMERICA INC +1
Evaluating method, evaluating apparatus, evaluating program product, evaluating system, and terminal apparatus for colorectal cancer
PendingUS20190228844A1Reliable informationMolecular entity identificationOmicsSerotoninHydroxyproline
An evaluating method includes an evaluating step of evaluating a state of colorectal cancer for a subject to be evaluated using (i) a concentration value of at least one metabolite of bABA, N-Me-bABA, 1-Me-His, aABA, Aminoadipic acid, bAiBA, Cadaverine, Ethylglycine, GABA, Homoarginine, Hypotaurine, Kinurenine, N6-Acetyl-L-Lys, Putrescine, Serotonin, Spermidine, Spermine, ADMA, Homocitrulline, 3-Me-His, Hydroxyproline, Phosphoethanolamine, SDMA, Acylcarnitine (13:1), and EPA in blood of the subject or (ii) a value of a formula calculated using the concentration value of the metabolite and the formula including an explanatory variable to be substituted with the concentration value of the metabolite.
Owner:AJINOMOTO CO INC
Mutant microorganism producing l-aspartic acid derivatives, and method for producing l-aspartic acid derivatives using same
The present invention relates to a mutant organism having the ability to produce aspartic acid derivatives, wherein a gene encoding the glyoxylate shunt regulator and a gene encoding fumarase are deleted and a gene encoding aspartase is overexpressed compared to that in a wild-type strain, and to a method for producing L-aspartic acid derivatives using the same. According to the present invention, various aspartic acid derivatives, including L-alanine, 3-aminopropionic acid, threonine, 1,3-diaminopropane, lysine, methionine, 3-hydroxypropionic acid, cadaverine, 5-aminovaleric acid, etc., can be produced by biological methods.
Owner:KOREA ADVANCED INST OF SCI & TECH
Pentiptycene derivative as well as preparation method and application thereof in polyamine detection
InactiveCN109836414AFacilitate charge transferLower energy levelOrganic chemistryFluorescence/phosphorescencePolyamine CompoundMetabolite
The invention relates to a pentiptycene derivative represented by formula I, n is 1 or 3, and R is H or OMe. The invention also relates to a preparation method of the pentiptycene derivative and a preparation method, application thereof in polyamine compound detection, a fluorescent sensor comprising the pentiptycene derivative represented by the formula I and a method for detecting polyamine compounds. The polyamine compounds are particularly putrescine, cadaverine, spermidine and spermine. The pentiptycene derivative shown in the formula I does not emit light basically in a free state, and emits fluorescent light in the presence of the polyamine compounds. The pentiptycene derivative has an aromatic group comprising a donor-pi-receptor manner and a fluorescent group having an aggregation-induced luminescence effect, so that the fluorescence intensity is very obvious. Therefore, the pentiptycene derivative represented by the formula I can be used as a fluorescent probe of the polyamine compounds, especially can be used for detecting biological polyamine compounds in an animal and a plant body, and can be used as an effective means for researching a biological model and detecting life metabolites.
Owner:PEKING UNIV SHENZHEN GRADUATE SCHOOL
A method for simultaneous and rapid detection of seven biogenic amines in liquor by ultra-high performance convergence chromatography coupled with QDA
ActiveCN105866302BEasy to useMeet the low limit detection requirementsComponent separationTesting beveragesMass spectrometry detectorCadaverine
The invention discloses a method for utilizing an ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit and belongs to the technical field of detection. The method is characterized by the following steps: after extracting a to-be-detected wine sample with acetonitrile, taking BEH C18 bonded phase chromatographic column as a separating column and carbon dioxide (A)+ isopropanol (B) as flowing phase for performing gradient eluting, and adopting a QDa mass spectrometry detector for detecting after separating the sample through the ultra-effective bonded phase chromatography (UPC2). The method provided by the invention is simple, quick, accurate, reliable and applicable to the simultaneous detection for the contents of seven biogenic amines (histamine, putrescine, cadaverine, phenylethylamine, spermidine, tyramine and octopamine) in white spirit.
Owner:ANHUI GUJING DISTILLERY +1
AuNPs-p-Ti3C2Tx composite material as well as preparation method and application thereof
ActiveCN113702453AImprove transmission efficiencyLarge specific surface areaMaterial nanotechnologyElectrophoretic coatingsMaterials preparationPork meat
The invention provides an AuNPs-p-Ti3C2Tx composite material as well as a preparation method and application thereof, and belongs to the technical field of composite material preparation, sensor preparation and meat product quality detection. According to the invention, the AuNPs-p-Ti3C2Tx composite material is firstly prepared, then the bimolecular imprinting sensor is prepared based on the composite material, the bimolecular imprinting sensor is easy and convenient to operate, rapid and sensitive, excellent in selectivity, high in stability and extremely low in detection limit, the sum of the content of cadaverine and the content of putrescine can be detected at the same time, and the bimolecular imprinting sensor has good application in evaluation of pork freshness.
Owner:JIANGSU UNIV
Three-dimensional flexible SERS (Surface Enhanced Raman Scattering) substrate and putrescine and cadaverine detection method based on substrate
PendingCN114354572AIncrease the areaHuge development potentialRaman scatteringHeterojunctionCadaverine
The invention discloses a three-dimensional flexible SERS (Surface Enhanced Raman Scattering) substrate and a putrescine and cadaverine detection method based on the substrate. The substrate is prepared by the following method: 1) preparing clustered ZnO nanorods on a PVDF (Polyvinylidene Fluoride) film; 2) coating the ZnO nanorod with a gold film; 3) after modification by polyethyleneimine, fixing gold nanoparticles on the surface of the ZnO nanorod; and (4) Au < 3 + > is reduced, AuNPs further grows, and the three-dimensional flexible SERS substrate is obtained. According to the preparation method, the 3D rose dendritic nanostructure is prepared through in-situ chemical growth of AuNPs on the surface of the ZnO nanorod, and the SERS activity of the heterostructure is remarkably enhanced through the LSPR effect and the CT effect; the p-MBA modified 3D rose dendritic SERS substrate can realize high detection sensitivity and rapid detection of putrescine and cadaverine in a sample, and has a good application prospect in instant detection of meat products.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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