A method for simultaneous and rapid detection of seven biogenic amines in liquor by ultra-high performance convergence chromatography coupled with QDA

An ultra-efficient confluence and biogenic amine technology, applied in the fields of detection and alcohol analysis, can solve the problems of instability, trouble, and toxicity of benzoyl chloride in liquid chromatography-derivatization method, achieve excellent separation effect, simple and fast operation, good repeatability
CN105866302BActive Publication Date: 2017-12-15ANHUI GUJING DISTILLERY +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ANHUI GUJING DISTILLERY
Publication Date
2017-12-15

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Abstract

The invention discloses a method for utilizing an ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit and belongs to the technical field of detection. The method is characterized by the following steps: after extracting a to-be-detected wine sample with acetonitrile, taking BEH C18 bonded phase chromatographic column as a separating column and carbon dioxide (A)+ isopropanol (B) as flowing phase for performing gradient eluting, and adopting a QDa mass spectrometry detector for detecting after separating the sample through the ultra-effective bonded phase chromatography (UPC2). The method provided by the invention is simple, quick, accurate, reliable and applicable to the simultaneous detection for the contents of seven biogenic amines (histamine, putrescine, cadaverine, phenylethylamine, spermidine, tyramine and octopamine) in white spirit.
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Description

technical field

[0001] The invention relates to the technical field of detection, in particular to a method for simultaneously rapidly detecting seven biogenic amines in liquor by using ultra-high-efficiency convergence chromatography connected in series with a QDa detector, and belongs to the technical field of wine analysis. Background technique

[0002] Biogenic amines are biologically active substances, mainly including β-phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermidine, etc., which exist in a variety of foods, especially fermented foods (such as cheese, wine, etc.) , beer, rice wine, fermented sausage, condiments), aquatic products and meat products, etc. Biogenic amines, especially histamine, are inherently toxic, so they are a major indicator to measure the freshness of meat and aquatic products and the hygiene conditions in the wine production process.

[0003] The current GB standard is detected by colorimetric method or liquid ...

Claims

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