178 results about "Histamine" patented technology

The invention provides a colorimetric / fluorescent dual-mode sensor, a preparation method of the colorimetric / fluorescent dual-mode sensor and application of the colorimetric / fluorescent dual-mode sensor in histamine detection. The colorimetric / fluorescent dual-mode sensor comprises a horseradish peroxidase-loaded metal organic framework (HRP-AIE-MOF), hydrogen peroxide and a chromogenic substrate o-phenylenediamine (OPD), horse radish peroxidase (HRP) in the HRP-coated AIE-MOF can oxidize colorless OPD into yellow 2, 3-diaminophenazine (DAP), an ultraviolet absorption peak is generated at 425 nm, a strong fluorescence emission peak is generated at 555 nm, the HRP-coated AIE-MOF has a strong fluorescence emission peak at 450 nm, and the HRP-coated AIE-MOF and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of natural enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, and high-sensitivity histamine detection is realized.

A method of determining the likelihood that a subject with cancer will respond to an immunotherapy comprising a checkpoint inhibitor. The method comprises detecting the level of histamine in a biological sample obtained from the subject. Subjects determined to have a low likelihood of responding to the immunotherapy can be administered an alternative therapy or administered an antihistamine in conjunction with the immunotherapy. Methods of treating cancer patients are also provided, as are kits and systems for histamine detection, recordation, and responder status determination.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for treating rhinitis. The traditional Chinese medicine composition comprises platycodon grandiflorum, semen armeniacae amarae, citrus chachiensis hortorum, fructus aurantii, aster, ephedra, radices trichosanthis, thunberg fritillary bulb, liquorice and loquat leaves. The traditional Chinese medicine composition can be used for remarkably relieving sneezing symptoms caused by allergic rhinitis, reducing histamine and specific IgE levels in serum and improving the pathological change degree of nasal mucosa tissues, and has a wide application prospect.

The invention relates to a composition with moisturizing, soothing and repairing effects and application thereof. The composition with the moisturizing, soothing and repairing effects comprises a pomelo fruit extract, a capparis spinosa fruit extract and hydroxyphenyl propionamide benzoic acid. Three active components, namely the pomelo fruit extract, the capparis spinosa fruit extract and the hydroxyphenyl propionamide benzoic acid, are creatively combined for use, wherein the pomelo fruit extract can inhibit release of inflammatory factors, reduce ultraviolet-induced DNA damage and inflammatory response, neutralize free radicals and reduce oxidative stress damage; wherein the capparis spinosa fruit extract can quickly moisturize, enhance cell viability and promote enhancement of a skin barrier; wherein hydroxyphenyl propionamide benzoic acid can block symptoms such as skin redness and swelling, pruritus and pain caused by the fact that histamine activates a TRPV1 pathway. The three components are matched with one another, have a synergistic effect in the aspects of moisturizing, relieving the skin and repairing the skin barrier, and have an excellent effect when being applied to cosmetics with related effects.

The invention provides a composition with a soothing effect, a preparation scheme of the composition and application of cosmetics. The composition comprises the following components in percentage by mass: 35-50% of actinidia chinensis fruit water, 2-10% of a radix gentianae extract, 1-9% of a citron fruit extract, 1-4% of a dendrobium stem extract, 1-5% of an oat extract and the balance of butanediol. All the components in the composition cooperate with one another, the multi-dimensional synergistic effect of resisting inflammation, resisting histamine, repairing a barrier, resisting oxidation and adjusting micro-ecology can be achieved, and the composition has the excellent relieving effect.

The invention belongs to the technical field of livestock feeds, and particularly relates to a novel chitosan composite nutritional breeding feed and a preparation method thereof. The novel chitosan composite nutritional breeding feed is composed of a basal feed and an additive, and the additive is prepared from the following raw materials in parts by weight: 3 to 5 parts of chitosan, 0.7 to 1.5 parts of sunflower bud extract, and 0.5 to 0.9 part of beta-alanyl-L-histidine. The chitosan, the sunflower bud extract and the beta-alanyl-L-histidine in the breeding feed are matched for use, so that the immunity of cattle and sheep can be effectively improved, the growth performance of the cattle and the sheep is improved, the breeding benefit is remarkably improved, diseases are reduced, and the nutritional requirements of animals such as the cattle and the sheep are fully met.

The invention relates to an SERS (Surface Enhanced Raman Scattering) active nano composite material for detecting trace histamine in tears, SERS sensing test paper as well as a preparation method and application, and belongs to the field of biosensing and spectral analysis. At least one of the problems of low detection sensitivity, poor anti-pollution capacity, low selectivity, incapability of realizing on-site rapid and instant analysis and the like of trace histamine in tears in the prior art is solved. The SERS active nano composite material is of a core-shell structure, and comprises a noble metal nano particle core, the surface of the core is coated with the hollow zeolite imidazate skeleton-67 shell layer, namely a ZIF-67 shell layer of a hollow structure; the Raman reporter molecule is fixed on the surface of the noble metal nanoparticle core. According to the invention, the detection sensitivity, anti-pollution capability and selectivity of trace histamine in tears are improved, and non-invasive rapid detection is realized.

The present invention relates to the field of microbial technology, and specifically discloses a recombinant microorganism and its application in the fermentation production of decarboxylated carnosine. The recombinant microorganism of the present invention has enhanced mdtK The starting strain is Escherichia coli capable of producing decarboxylated carnosine. Fermentation production using the recombinant microorganism of the present invention can increase the yield of decarboxylated carnosine without adding precursors such as L-histidine, histamine, or β-alanine during the production process, thereby providing an effective new method for the industrial production of decarboxylated carnosine.

The invention discloses application of bifidobacterium longum subsp. Infantis CCFM1269 in preparation of a product for relieving lactoglobulin allergy, and belongs to the technical field of microorganisms. The bifidobacterium longum subsp. Infantis CCFM1269 provided by the invention can relieve food allergy, and the food allergy can be relieved specifically as follows: (1) allergy symptom score and diarrhea occurrence rate can be relieved, and anus temperature and immune organ index can be recovered; (2) the immune response is regulated, the levels of histamine, total IgE, IgG1, beta-lactoglobulin specific IgE and proinflammatory factors IL-4 and IL-17A in serum are reduced, the levels of IFN-gamma and IL-10 are improved, and the immune balance is recovered; (3) inflammation of lung and jejunum tissues is relieved; (4) improving the oxidative stress state; (5) the intestinal barrier function is enhanced; and (6) recovering the diversity of intestinal flora, and adjusting the relative abundance of key bacteria genus such as Alloprevotella and the like.

Described herein are compositions and methods for treating or preventing skin or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., Demodex), helminths, protozoa (e.g., amebas (e.g., Acanthamoeba or Cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. The compositions and methods include compounds that exhibit immunological activity, keratolytic activity, antihistamine activity, anti-inflammatory activity, and / or other desirable activities. Topical administration of the compositions to the eyelid margin or surrounding area provides therapeutic benefit to patients with ocular surface disorders.

This invention relates to the crystal form of vonorazine pyroglutamate and its preparation method. The vonorazine pyroglutamate crystal form obtained by this invention not only has good solubility but also excellent storage stability; in vitro activity experiments show that crystal form I of this invention is effective against H+. + K + The inhibitory effect of ATPase is comparable to that of TAK-438; animal experiments show that the crystal form of the present invention, when administered intravenously, can significantly inhibit histamine-induced gastric acid secretion in rats, and can exert its pharmacological effect faster than that of TAK-438 administered duodenally.

The invention belongs to the technical field of medicine detection, and particularly relates to an LCMS (Liquid Chromatography Mass Spectrometry) detection method for histamine in prednisolone. Respectively injecting the reference solution, the test solution and the blank solution into an LCMS (Liquid Chromatography Mass Spectrometer), recording a spectrogram, and calculating the content of histamine in prednisolone by adopting an external standard method; wherein the detection condition of the LCMS comprises a mobile phase, and the mobile phase adopts acetonitrile as a mobile phase A and a 10mM ammonium formate aqueous solution containing 0.1 vol% of formic acid as a mobile phase B. The method is good in reproducibility and stability and high in precision.

The invention belongs to the technical field of food safety detection, and particularly relates to a histamine molecular imprinting electrochemical sensor based on a carbon-based nano composite material and a preparation method and application of the histamine molecular imprinting electrochemical sensor. Aiming at the problems of poor selectivity, high detection limit or complicated operation and the like of the existing histamine detection technology, a Co3O4 / N-HCS (cobaltosic oxide / nitrogen-doped hollow carbon sphere) composite material is prepared by adopting a solvothermal method, o-phenylenediamine (o-PD) is taken as a functional monomer, a molecularly imprinted polymer membrane (MIP) is constructed on the surface of a glassy carbon electrode (GCE) by adopting an electropolymerization method, and the histamine detection electrode is prepared. The MIP / Co3O4 / N-HCS / GCE sensor with specific recognition sites on histamine is formed. The sensor has high selectivity, high sensitivity and good stability, is simple and convenient to operate and rapid in detection, can efficiently realize accurate detection of histamine in food, and is suitable for safety monitoring scenes of various foods such as fermented food, aquatic products and dairy products.

The invention relates to the technical field of histamine extraction, in particular to histamine removal and extraction equipment and method for apramycin sulfate, the histamine removal and extraction equipment comprises a drying box with a feeding port and a discharging port, an opening and closing unit used for controlling opening and closing of the discharging port is arranged at the discharging port, and a plurality of heating plates sequentially arranged in the horizontal direction are arranged in the drying box; a heating cavity is defined by every two adjacent heating plates; a sliding plate and a driving unit used for driving the sliding plate to move in the length direction of the heating cavity are arranged in the drying box, a plurality of air injection units and a plurality of vertically-arranged spiral conveying units are arranged on the sliding plate, and every two sets of air injection units correspond to one heating cavity and vertically blow air to the cavity walls of the left side and the right side of the heating cavity correspondingly. And the spiral conveying units are in one-to-one correspondence with the heating cavities and extend into the corresponding heating cavities. The drying efficiency of the crystals can be improved.

The application belongs to the technical field of fluorescent nanomaterials, and provides nitrogen-doped carbon dots, a preparation method thereof and application of the nitrogen-doped carbon dots in colorimetric / fluorescent dual-mode visual detection of pH and histamine, to solve the problem that regular colorimetric signals of current carbon dots are difficult to achieve, which limits the application of the carbon dots in biomedicine and photoelectric devices. The nitrogen-doped fluorescent carbon dots are synthesized by one-step hydrothermal reaction of o-phenylenediamine and 2,6-pyridinedicarboxylic acid, water and hydrochloric acid. The preparation method is simple and fast, the synthesized carbon dots have good water solubility and biocompatibility, stable optical properties, high fluorescence quantum yield, can quickly and visually detect intracellular pH and histamine in food spoilage process, have excellent selectivity and anti-interference ability, fast response speed, high sensitivity, and can realize on-site and instant detection by means of a smart phone and a test strip.

The present invention relates to a bispecific anti-tumor antigen / anti-HSG antibody having screened light and heavy chain variable domains, which is capable of improving the pre-targeting of tumors for the specific delivery of therapeutic or diagnostic agents. The antibodies have a significant affinity for tumor antigens and stay at desired sites for a sufficient time. Antibodies that do not bind to the antigen can be rapidly cleared from the body, minimizing exposure of normal tissue. A humanized anti-HSG antibody is bound to each of a group containing histamine-succinyl-glycine (HSG) and a tumor antigen with high affinity. The bispecific antibodies can be further Fc silenced, resulting in additional properties, such as reduced binding to Fc [gamma] R and FcRn, thereby modulating effector function and regulating half-life. These antibodies are useful in the diagnosis and treatment of subjects suffering from malignancies.

The invention discloses a near-infrared ratio type fluorescent probe for rapidly and reversibly detecting histamine (HA), a preparation method of the fluorescent probe and application of the fluorescent probe in dynamic cell imaging, and belongs to the technical field of fluorescence imaging and biosensing. The preparation method comprises the following specific steps: synthesizing aza-BODIPY molecules (1a-1d, 2a-2d) with different halogenations, and meanwhile, connecting the aza-BODIPY molecules with hydroxyl groups at 3 and 5 sites with a hydrophilic PEG (Polyethylene Glycol) fragment and halogen to obtain an HA probe HAPCyto with good water solubility; in addition, the hydroxyl aza-BODIPY molecules are continuously subjected to substitution, halogenation and hydrolysis reaction to obtain carboxyl-containing aza-BODIPY molecules, then the aza-BODIPY molecules are subjected to amidation reaction by utilizing the site, and different organic fragments are respectively connected to obtain a cell membrane targeted HA probe HAPPM, a golgi apparatus targeted HA probe HAPGA and a HaloTag HA fluorescent probe HAPHaloTag. When HA exists in a solution, the developed probe can realize rapid reversible detection of the near-infrared ratio of histamine, also shows good reversible property in mast cells, and can realize dynamic monitoring of histamine in cells.

[Problem] To provide a substance having an activity of antagonizing the binding of histamine to histamine-H3 receptor, or having an activity of inhibiting the homeostatic activity of histamine-H3 receptor. [Means for Solution] A compound of the following formula (I) is provided: [wherein X, Y, Z and W each independently represent a methine group optionally having 1 or 2 substituents, or a nitrogen atom (provided that a case where all of X, Y, Z and W are an optionallysubstituted methine group); A, B and D represent C(0)-, etc.; Q represents a methine group or a nitrogen atom; R represents an optionallysubstituted group of the following formula (II-1): (wherein R7 and R8 each independently represent a lower alkyl group, etc.)].

The application discloses a kind of composite plant extract and its application, skin external preparation.The raw material of the composite plant extract includes hemerocallis fulva flower and red clover, and the weight ratio of hemerocallis fulva flower and red clover is (1:5)~(5:1).The plant extract of hemerocallis fulva flower and red clover of the application can inhibit the release of histamine, thereby reducing the occurrence of inflammation and infection;The plant extract of the application cooperates synergistically, and compared with each plant alone, the histamine inhibition effect of the plant extract of the application is significantly improved;The plant extract of the application uses pure natural plant as raw material, and has low side effect and high safety;The plant raw material used in the application is widely planted in China, and the resource is easy to obtain, the price is cheap, and the cost is low.

The invention discloses a multi-copper oxidase mutant sourced from Psychrobacter sp. And capable of efficiently degrading biogenic amine and application of the multi-copper oxidase mutant. According to the multi-copper oxidase mutant, the 183rd site of an amino acid sequence shown in SEQ ID NO.1 is mutated into histidine from phenylalanine, the 221st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into aspartic acid from asparagine, the 261st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into glutamic acid from glutamine, and the 292nd site of the amino acid sequence shown in SEQ ID NO.2 is mutated into arginine from lysine. Compared with wild type multi-copper oxidase, the multi-copper oxidase mutant disclosed by the invention has a good degradation effect on tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, and the degradation rate on the spermidine and the spermine is increased to 100%, so that the multi-copper oxidase mutant has a good application prospect in food fermentation safety production.

The present invention is directed to a pharmaceutical composition comprising a pharmaceutically effective amount of guaifenesin, naproxen and at least one further active which is selected to be an antitussive, a decongestant or an antihistamine.

An antihistamine compound having the structure of formula (I), a pharmaceutically acceptable salt thereof, a method for preparing the same, and use of the antihistamine compound having the structure of formula (I) and a pharmaceutically acceptable salt thereof in the treatment of diseases such as seasonal / perennial allergic rhinitis, allergic conjunctivitis, and urticaria. JPEG2025519827000061.jpg85170

To provide a method for treating animal protein and a pet food product containing the animal protein.SOLUTION: A method of treating one or more sources of proteins, the method comprising adding one or more base components to the source of proteins, wherein the treated source of proteins has a histamine level of less than about 300ppm is provided.SELECTED DRAWING: None

The invention belongs to the technical field of spectral analysis, and particularly relates to a method for enhancing low-frequency Raman signals of biomolecules by using a resonance Raman technology. Aiming at biomolecules with characteristic low-frequency signals, such as amino acids, neurotransmitters, lipids and the like, the invention establishes a systematic resonance enhancement detection method for the first time. Compared with an existing surface enhanced Raman technology, the scheme gets rid of dependence on a specific nano substrate, and universal detection of various biomolecules is realized by optimizing an excitation wavelength selection algorithm (such as a 1 / e intensity range criterion) and a substrate treatment process (such as a dry oxygen silicon oxide substrate). Experiments prove that the detection limit of the method on molecules such as histamine and dopamine is reduced by one order of magnitude compared with that of a conventional Raman technology.

This invention provides an oligopeptide cyclic peptide and its applications. The cyclic peptide is obtained by truncating the linear Hst1 sequence to the shortest possible sequence that retains Hst1 activity. Then, a triazole ring-containing oligopeptide is prepared by a cycloaddition reaction under specific conditions targeting this shortest sequence. Its molecular weight is only 1 / 3 that of linear histamine, and its biological activity is 15 times that of linear histamine. To achieve the same activity, the cost of the oligopeptide cyclic peptide is only 1 / 100 that of linear histamine, and the yield is significantly higher than that of cyclic peptides directly cyclized with amide bonds. It also exhibits significantly improved stability and resistance to biodegradation. The entire cyclization process of this oligopeptide cyclic peptide is mild, simple, and efficient, and can produce high-purity oligopeptide cyclic peptides. It can be used to prepare anti-aging and repairing skincare products, health products, or pharmaceuticals, and has excellent prospects for large-scale industrialization.

The invention discloses a high-performance biosensor based on a g-C3N4 / TiO2 heterojunction as well as a preparation method and application of the high-performance biosensor, and belongs to the technical field of biosensing and food safety detection. The sensor is constructed by taking a glassy carbon electrode as a substrate and sequentially modifying a g-C3N4 / TiO2 heterojunction composite material and diamine oxidase (DAO). The g-C3N4 / TiO2 heterojunction is prepared through a one-step calcination method, a type II p-n junction structure is formed, photo-generated charge separation and interface electron transfer are effectively promoted, and the conductivity, catalytic activity and enzyme immobilization capacity of the electrode are remarkably improved. The invention discloses a synergistic mechanism of heterojunction interface enhanced histamine selective adsorption and catalytic reaction through density functional theory calculation. The sensor shows excellent detection performance on histamine: the linearity is good in a range of 0.05-10 mM, and the detection limit is as low as 0.075 M; the selectivity is still higher than 93% in the presence of an interferent with ten times of concentration; when the method is applied to detection of actual samples of beef, mutton, fish and chicken, the adding standard recovery rate is 93.60%-101.8%, and the relative standard deviation is smaller than 5%. The sensor has the advantages of high sensitivity, good selectivity, strong anti-interference capability, good reproducibility and the like, and a reliable new method is provided for rapid and accurate screening of histamine in food.

The invention discloses lactobacillus plantarum XHQ-007 capable of degrading biogenic amine and application of the lactobacillus plantarum XHQ-007. The lactobacillus plantarum XHQ-007 is preserved in Guangdong Microbial Culture Collection Center on March 14, 2025, and the preservation number is GDMCC No: 66016. The lactobacillus plantarum XHQ-007 can be used for degrading biogenic amine. The strain has no biogenic amine synthesis capability, has a biogenic amine degradation function, can effectively reduce the content of biogenic amine in fermented food, has a tyramine degradation rate of 79.94%, a histamine degradation rate of 59.01% and a tryptamine degradation rate of 75.23%, can effectively control the content of biogenic amine in the production process of fermented food such as fruit wine and the like, and has a good application prospect. And a healthy and safe fermented product is provided for consumers.

The present application provides a rapid detection system for allergic conjunctivitis and its application. Specifically, the present application provides a rapid detection system for detecting allergic conjunctivitis, which comprises: (A) a subject user terminal; and (B) a background processing end. The rapid detection system of the present application can timely, efficiently and accurately evaluate the incidence and severity of allergic conjunctivitis based on the detection results of the levels of IgE and histamine in tears, realize the home detection of allergic conjunctivitis, reduce misdiagnosis and avoid the aggravation of allergic conjunctivitis due to medical delay, which is helpful to improve the medical efficiency and has a broad clinical application prospect.

The application provides application of an adenosine A2B receptor as a target in a medicine for preventing and / or treating itch, and belongs to the technical field of biological medicines. Based on previously reported single-cell RNA sequencing data of a mouse dorsal root ganglion (DRG), expression of genes encoding main ligand ion channels is detected, Adora2b highly expressed in Neuron_10 is detected, and it is indicated that Adora2b and a receptor (adenosine A2B receptor) of Adora2b can participate in regulating itch. Experiments prove that the activity of the adenosine A2B receptor can be effectively blocked to prevent a histamine-induced scratching behavior of a mouse, and it is indicated that the A2B receptor participates in histamine-induced itch sensation. The application also provides application of a reagent for inhibiting biological functions of the adenosine A2B receptor in preparation of a medicine for preventing and / or treating itch, and provides a new means for prevention and treatment of itch.

The present invention relates to a recombinant human diamine oxidase (DAO) with reduced glycosaminoglycan binding affinity, said DAO comprising at least one amino acid modification in the glycosaminoglycan (GAG)-binding domain. The present invention further relates to the use of DAO in the treatment of conditions associated with excess histamine, particularly in the treatment of chronic allergic diseases, more particularly in the treatment of anaphylaxis, anaphylactic shock, chronic urticaria, acute urticaria, asthma, hay fever, allergic rhinitis, allergic conjunctivitis, histamine poisoning, headache, atopic dermatitis, inflammatory diseases, mastocytosis, mast cell activation syndrome (MCAS), pre-eclampsia, hyperemesis gravidarum, preterm labor, peptic ulcer, acid reflux, pruritus, and sepsis.