370 results about "Histamine" patented technology

The invention relates to the technical field of microorganisms, and particularly discloses a recombinant microorganism and application thereof in fermentation production of decarboxylated carnosine. Compared with an original strain, the recombinant microorganism disclosed by the invention has the advantages that the expression of the mdtK gene is enhanced; the starting strain is escherichia coli capable of producing decarboxylated carnosine. When the recombinant microorganism is used for fermentation production, the yield of the decarboxylated carnosine can be increased, L-histidine, histamine or beta-alanine and other precursors do not need to be added in the production process, and an effective novel method is provided for industrial production of the decarboxylated carnosine.

The invention provides a colorimetric / fluorescent dual-mode sensor, a preparation method of the colorimetric / fluorescent dual-mode sensor and application of the colorimetric / fluorescent dual-mode sensor in histamine detection. The colorimetric / fluorescent dual-mode sensor comprises a horseradish peroxidase-loaded metal organic framework (HRP-AIE-MOF), hydrogen peroxide and a chromogenic substrate o-phenylenediamine (OPD), horse radish peroxidase (HRP) in the HRP-coated AIE-MOF can oxidize colorless OPD into yellow 2, 3-diaminophenazine (DAP), an ultraviolet absorption peak is generated at 425 nm, a strong fluorescence emission peak is generated at 555 nm, the HRP-coated AIE-MOF has a strong fluorescence emission peak at 450 nm, and the HRP-coated AIE-MOF and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of natural enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, and high-sensitivity histamine detection is realized.

The invention relates to a plasma metabolite composition for auxiliary diagnosis of sub-clinical mastitis of dairy cows and application of the plasma metabolite composition. The plasma metabolite combination is a first combination and / or a second combination, and the first combination is composed of glyceryl monolinoleate, 2-methylthiophene, L-cystine, (S)-(+)-citramalic acid, 9-hydroxy-10, 12-octadecadienoic acid, 16-hydroxyhexadecanoic acid, tyrosine hydroxyproline, stearoyl serotonin and histamine; the first composition is composed of 4, 5-dimethyl-2, 4, 5-trimethyloxazole, 12, 13-epoxy octadecadienoic acid, 2-cyclotetradecene-1-ketone, 4 [alpha]-carboxyl-5 [alpha]-cholest-8-ene-3 [beta]-alcohol, N-arachidonyl dopamine d8, 9 (Z) and 11 (E)-conjugated linoleic acid, and the second composition is composed of 2, 5-dihydro-2, 4, 5-trimethyloxazole, 12, 13-epoxy octadecadienoic acid, 2-cyclotetradecene-1-ketone, 4 [alpha]-carboxyl-5 [alpha]-cholest-8-ene-3 [beta]-alcohol, N-arachidonyl dopamine A product prepared based on the plasma metabolite composition and used for auxiliary diagnosis of cow subclinical mastitis is simple in use method and high in accuracy, can be used for assisting a milk somatic cell number method to diagnose cow subclinical mastitis, and reduces the probability of false negative and false positive.

A method of determining the likelihood that a subject with cancer will respond to an immunotherapy comprising a checkpoint inhibitor. The method comprises detecting the level of histamine in a biological sample obtained from the subject. Subjects determined to have a low likelihood of responding to the immunotherapy can be administered an alternative therapy or administered an antihistamine in conjunction with the immunotherapy. Methods of treating cancer patients are also provided, as are kits and systems for histamine detection, recordation, and responder status determination.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for treating rhinitis. The traditional Chinese medicine composition comprises platycodon grandiflorum, semen armeniacae amarae, citrus chachiensis hortorum, fructus aurantii, aster, ephedra, radices trichosanthis, thunberg fritillary bulb, liquorice and loquat leaves. The traditional Chinese medicine composition can be used for remarkably relieving sneezing symptoms caused by allergic rhinitis, reducing histamine and specific IgE levels in serum and improving the pathological change degree of nasal mucosa tissues, and has a wide application prospect.

The present invention relates to the field of pharmaceutical chemistry, and discloses a salt of carebastine and its use, which solves the problems of the prior art, such as poor solid form of carebastine, many impurities, instability, difficulty in purification, difficulty in mass synthesis, and inapplicability to medicinal use. The salt of carebastine of the present invention includes, but is not limited to, acid salts or base salts, particularly potassium salt, sodium salt, methanesulfonate salt and p-toluenesulfonate salt. The salt of carebastine of the present invention is used in the preparation of drugs for histamine H1 receptor antagonists. The salt of carebastine of the present invention has the characteristics of easy purification, high stability, simple process, easy industrial production, and good hygroscopicity and convenient storage. And the salt of the present invention can enter the body quickly and exert its medicinal effect, has good oral absorption, and is superior to ebastine in both safety and individual variation, and is a promising anti-allergic disease drug for development.

The invention belongs to the technical field of X-ray detection, and particularly relates to lead-free two-dimensional double perovskite, a preparation method of the lead-free two-dimensional double perovskite and an X-ray detector manufactured based on the perovskite. The lead-free two-dimensional double perovskite is applied to X-ray detection. According to the preparation method, histamine dihydrochloride (C5H11Cl2N3Cl), silver chloride (AgCl) and bismuth trioxide (Bi2O3) are mainly used as raw materials, a mixed solution of hydrobromic acid (HBr) and hypophosphorous acid (H2PO2) is used as a solvent, a single crystal material ((C5H9N3) 2AgBiBr8 single crystal) is prepared through a hydrothermal method, and the two sides of the single crystal are coated with silver paste to serve as electrodes, so that the direct type X-ray detector is obtained. The preparation method disclosed by the invention has the characteristics of simplicity, easiness in operation, low requirement on equipment, low cost, low energy consumption and suitability for expanded production.

The disclosure is generally related to therapeutic compositions and methods for use in the treatment of somatosensory conditions, including pain and pruritis. In some aspects, the compositions comprise (i) a nonsteroidal anti-inflammatory drug (NSAID), an opioid, or a combination thereof, and (ii) one or more transdermal delivery agents. In some aspects, the compositions comprise (i) an antihistamine, a kappa opioid receptor agonist, an anti-pruritogen, or a combination thereof, and (ii) one or more transdermal delivery agents.

The invention belongs to the technical field of biology, and particularly discloses application of laccase AmLac in degradation of biogenic amines. The amino acid sequence of the laccase is shown as SEQ ID NO.1. The laccase has different degrees of degradation effects on the biogenic amines such as histamine, spermidine, tyramine, phenylethylamine and putrescine, and the degradation rates of the histamine and the putrescine both reach 100%. The enzyme is stable in enzymatic property, has alcohol tolerance and acid tolerance, has certain adaptive capacity to food matrixes, and can effectively degrade biogenic amines in wine and reduce potential hazards of the biogenic amines to human bodies, so that the safety and stability of products are improved. Therefore, the AmLac has a wide application prospect in the food industry, and especially when the AmLac is used for treating food containing high biogenic amine, the safety quality of the food can be remarkably improved.

The invention relates to a composition with moisturizing, soothing and repairing effects and application thereof. The composition with the moisturizing, soothing and repairing effects comprises a pomelo fruit extract, a capparis spinosa fruit extract and hydroxyphenyl propionamide benzoic acid. Three active components, namely the pomelo fruit extract, the capparis spinosa fruit extract and the hydroxyphenyl propionamide benzoic acid, are creatively combined for use, wherein the pomelo fruit extract can inhibit release of inflammatory factors, reduce ultraviolet-induced DNA damage and inflammatory response, neutralize free radicals and reduce oxidative stress damage; wherein the capparis spinosa fruit extract can quickly moisturize, enhance cell viability and promote enhancement of a skin barrier; wherein hydroxyphenyl propionamide benzoic acid can block symptoms such as skin redness and swelling, pruritus and pain caused by the fact that histamine activates a TRPV1 pathway. The three components are matched with one another, have a synergistic effect in the aspects of moisturizing, relieving the skin and repairing the skin barrier, and have an excellent effect when being applied to cosmetics with related effects.

The invention provides a composition with a soothing effect, a preparation scheme of the composition and application of cosmetics. The composition comprises the following components in percentage by mass: 35-50% of actinidia chinensis fruit water, 2-10% of a radix gentianae extract, 1-9% of a citron fruit extract, 1-4% of a dendrobium stem extract, 1-5% of an oat extract and the balance of butanediol. All the components in the composition cooperate with one another, the multi-dimensional synergistic effect of resisting inflammation, resisting histamine, repairing a barrier, resisting oxidation and adjusting micro-ecology can be achieved, and the composition has the excellent relieving effect.

The invention belongs to the technical field of livestock feeds, and particularly relates to a novel chitosan composite nutritional breeding feed and a preparation method thereof. The novel chitosan composite nutritional breeding feed is composed of a basal feed and an additive, and the additive is prepared from the following raw materials in parts by weight: 3 to 5 parts of chitosan, 0.7 to 1.5 parts of sunflower bud extract, and 0.5 to 0.9 part of beta-alanyl-L-histidine. The chitosan, the sunflower bud extract and the beta-alanyl-L-histidine in the breeding feed are matched for use, so that the immunity of cattle and sheep can be effectively improved, the growth performance of the cattle and the sheep is improved, the breeding benefit is remarkably improved, diseases are reduced, and the nutritional requirements of animals such as the cattle and the sheep are fully met.

The present invention aims to provide a composition for inhibiting histamine-N-methyltransferase. The present invention relates to a composition for inhibiting histamine-N-methyltransferase, containing L-ergothioneine or a salt thereof.

The invention relates to an SERS (Surface Enhanced Raman Scattering) active nano composite material for detecting trace histamine in tears, SERS sensing test paper as well as a preparation method and application, and belongs to the field of biosensing and spectral analysis. At least one of the problems of low detection sensitivity, poor anti-pollution capacity, low selectivity, incapability of realizing on-site rapid and instant analysis and the like of trace histamine in tears in the prior art is solved. The SERS active nano composite material is of a core-shell structure, and comprises a noble metal nano particle core, the surface of the core is coated with the hollow zeolite imidazate skeleton-67 shell layer, namely a ZIF-67 shell layer of a hollow structure; the Raman reporter molecule is fixed on the surface of the noble metal nanoparticle core. According to the invention, the detection sensitivity, anti-pollution capability and selectivity of trace histamine in tears are improved, and non-invasive rapid detection is realized.

The invention discloses a lactobacillus mucus CCFM1401 for repairing a skin barrier based on multiple target spots and a metagen of the lactobacillus mucus CCFM1401, and belongs to the technical field of microorganisms and medicines. The thallus lysate of the fermented lactobacillus mucus CCFM1401, provided by the invention, can be used for improving the cell viability of damaged keratinocytes and the expression of FLG, LOR, IVL, ZO-1, Occludin and CLDN gene levels in the aspect of external application; and viable bacteria and metabiotics (including thallus lysate and fermentation supernatant) of the fermented lactobacillus mucus CCFM1401 are orally taken, so that TEWL can be reduced, the water content of cuticle can be up-regulated, inflammatory reactions (such as skin histamine and serum IgE) can be reduced, and the skin barrier function and expression of structural protein can be up-regulated. Therefore, the fermented lactobacillus mucilaginosus has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The present invention relates to the field of microbial technology, and specifically discloses a recombinant microorganism and its application in the fermentation production of decarboxylated carnosine. The recombinant microorganism of the present invention has enhanced mdtK The starting strain is Escherichia coli capable of producing decarboxylated carnosine. Fermentation production using the recombinant microorganism of the present invention can increase the yield of decarboxylated carnosine without adding precursors such as L-histidine, histamine, or β-alanine during the production process, thereby providing an effective new method for the industrial production of decarboxylated carnosine.

The invention relates to the technical field of microorganisms, in particular to lactobacillus reuteri for improving allergic rhinitis and nasosinusitis as well as a product and application thereof. The lactobacillus reuteri NHNK-2001 disclosed by the invention is deposited in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2024800. Experiments show that the NHNK-2001 has the functions of inhibiting the growth of nasosinusitis related flora and biofilm formation, flocculating rhinitis pathogenic bacteria, promoting the growth of nasal cavity beneficial bacteria, inhibiting staphylococcus aureus subsp.aureus enterotoxin, degrading histamine, reducing histamine excitation, promoting nasal cavity epithelial cell barrier and reducing nasal cavity epithelial cell inflammation; in addition, a biological membrane formed by the NHNK-2001 thallus can increase the colonization ability to intestinal epithelial cells. The traditional Chinese medicine composition can be used for preparing products for improving allergic rhinitis and nasosinusitis.

The invention discloses a culture medium for producing tylosin through fermentation and a preparation method of the culture medium, and relates to the technical field of microbial fermentation. The culture medium comprises jerusalem artichoke powder, corn starch, sunflower seed cake meal, rapeseed meal, cottonseed cake meal, pea meal, sodium chloride, monopotassium phosphate, disodium hydrogen phosphate, ammonium nitrate and saccharides. According to the culture medium for producing the tylosin through fermentation, traditional fish meal is replaced with the vegetable protein, so that the content of histamine in the prepared tylosin is obviously reduced, all the components in the fermentation culture medium have a synergistic effect, the content of the prepared tylosin and the content of a component A in the tylosin are obviously increased, and the content of the component A in the tylosin is increased; wide application prospects are realized.

The invention discloses application of bifidobacterium longum subsp. Infantis CCFM1269 in preparation of a product for relieving lactoglobulin allergy, and belongs to the technical field of microorganisms. The bifidobacterium longum subsp. Infantis CCFM1269 provided by the invention can relieve food allergy, and the food allergy can be relieved specifically as follows: (1) allergy symptom score and diarrhea occurrence rate can be relieved, and anus temperature and immune organ index can be recovered; (2) the immune response is regulated, the levels of histamine, total IgE, IgG1, beta-lactoglobulin specific IgE and proinflammatory factors IL-4 and IL-17A in serum are reduced, the levels of IFN-gamma and IL-10 are improved, and the immune balance is recovered; (3) inflammation of lung and jejunum tissues is relieved; (4) improving the oxidative stress state; (5) the intestinal barrier function is enhanced; and (6) recovering the diversity of intestinal flora, and adjusting the relative abundance of key bacteria genus such as Alloprevotella and the like.

Described herein are compositions and methods for treating or preventing skin or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., Demodex), helminths, protozoa (e.g., amebas (e.g., Acanthamoeba or Cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. The compositions and methods include compounds that exhibit immunological activity, keratolytic activity, antihistamine activity, anti-inflammatory activity, and / or other desirable activities. Topical administration of the compositions to the eyelid margin or surrounding area provides therapeutic benefit to patients with ocular surface disorders.

The present invention provides the use of phenylquinolinone and flavonoid derivatives for neuropathic pain, and the phenylquinolinone and flavonoid derivatives include pharmaceutically acceptable salts thereof. The compound of the present invention is a histamine H3 receptor antagonist, which has a different mechanism of action from existing drugs and can be used in the preparation of drugs for treating neuropathic pain diseases. The effect of the compound of the present invention in treating neuropathic pain is better than amitriptyline, gabapentin, pregabalin and carbamazepine, indicating that the compound of the present invention has a better therapeutic effect, has the characteristics of small dosage, good efficacy, high bioavailability and safety, and compared with opioid drugs, the compound of the present invention is not addictive and is a very effective drug for treating neuropathic pain diseases. Therefore, the clinical application prospects are huge. The general structural formula is as follows:

The present invention relates to the technical field of plant compositions, and in particular to a plant anti-allergic, antipruritic and analgesic composition and its application. The present application discloses a plant anti-allergic, antipruritic and analgesic composition, which comprises the following raw materials by weight percentage: gentiana cyanus extract: 0.5%-5%, kava root extract: 1%-10%, prickly pear cactus leaf extract: 0.5%-5%, Centella asiatica extract: 1%-10%, and the balance is water. The present application provides a plant anti-allergic, antipruritic and analgesic composition, which achieves the effects of anti-inflammatory, anti-allergic, antipruritic and analgesic by inhibiting NO generation, inhibiting histamine pathways and inhibiting TRPV1 ion channels.

This invention relates to the crystal form of vonorazine pyroglutamate and its preparation method. The vonorazine pyroglutamate crystal form obtained by this invention not only has good solubility but also excellent storage stability; in vitro activity experiments show that crystal form I of this invention is effective against H+. + K + The inhibitory effect of ATPase is comparable to that of TAK-438; animal experiments show that the crystal form of the present invention, when administered intravenously, can significantly inhibit histamine-induced gastric acid secretion in rats, and can exert its pharmacological effect faster than that of TAK-438 administered duodenally.

The invention discloses a protective agent for lung injury caused by intestinal ischemia reperfusion, and relates to the technical field of lung injury protective agents. The method comprises the following steps: step a: a synthesis step: S1, a purification process of tripterine: stirring an obtained concentrated solution with silica gel, fully mixing the silica gel and the concentrated solution, and drying into dispersed particles to obtain a granular raw material for later use; verification is carried out by establishing a cell model and a biological model, lung cell coking can be effectively relieved, the lung can be protected against oxidative stress injury, when the biological model is modeled, an intestinal ischemia reperfusion injury model is established for a mouse by utilizing an active modeling operation, clinical lung injury conditions are simulated, and the model is used for simulating the lung injury condition. The tripterine derivative is given through oral administration or intravenous injection, and it is verified that the tripterine derivative has the effects of inhibiting lung cell coking, preventing cells from releasing histidine and various inflammatory mediators and avoiding further aggravation of inflammatory response and cell damage.

The invention belongs to the technical field of medicine detection, and particularly relates to an LCMS (Liquid Chromatography Mass Spectrometry) detection method for histamine in prednisolone. Respectively injecting the reference solution, the test solution and the blank solution into an LCMS (Liquid Chromatography Mass Spectrometer), recording a spectrogram, and calculating the content of histamine in prednisolone by adopting an external standard method; wherein the detection condition of the LCMS comprises a mobile phase, and the mobile phase adopts acetonitrile as a mobile phase A and a 10mM ammonium formate aqueous solution containing 0.1 vol% of formic acid as a mobile phase B. The method is good in reproducibility and stability and high in precision.

The invention belongs to the technical field of food safety detection, and particularly relates to a histamine molecular imprinting electrochemical sensor based on a carbon-based nano composite material and a preparation method and application of the histamine molecular imprinting electrochemical sensor. Aiming at the problems of poor selectivity, high detection limit or complicated operation and the like of the existing histamine detection technology, a Co3O4 / N-HCS (cobaltosic oxide / nitrogen-doped hollow carbon sphere) composite material is prepared by adopting a solvothermal method, o-phenylenediamine (o-PD) is taken as a functional monomer, a molecularly imprinted polymer membrane (MIP) is constructed on the surface of a glassy carbon electrode (GCE) by adopting an electropolymerization method, and the histamine detection electrode is prepared. The MIP / Co3O4 / N-HCS / GCE sensor with specific recognition sites on histamine is formed. The sensor has high selectivity, high sensitivity and good stability, is simple and convenient to operate and rapid in detection, can efficiently realize accurate detection of histamine in food, and is suitable for safety monitoring scenes of various foods such as fermented food, aquatic products and dairy products.

The invention discloses a method for rapidly identifying harmful components in pickled peppers, and belongs to the technical field of food detection. The method comprises the following steps: preparing a reference substance solution, namely preparing a standard substance stock solution and a biogenic amine mixed solution from putrescine, histamine, tyramine and cadaverine standard substances; preparing a test sample solution: preparing the test sample solution by adopting a test sample; the chromatographic conditions are as follows: C18 is used as a filling agent; carrying out gradient elution by taking isopropanol as a mobile phase A and 0.5-2wt% ammonia water as a mobile phase B; the detection wavelength is 254 nm; the column temperature is 25-35 DEG C; and the flow velocity is 0.2-1.0 ml / min. The method has the advantages that (1) the detection speed is high, and the gradient elution speed is high and is only 12 minutes; (2) the number of obvious miscellaneous peaks is small, and only one miscellaneous peak exists; and (3) the method has relatively high precision, relatively low detection limit and good reproducibility.

Disclosed is a method for producing a stabilized lyophilisate with plant-based diamine oxidase (DAO), comprising the following steps: Providing DAO-containing plants or DAO-containing parts thereof; Homogenizing the DAO-containing plants or DAO-containing parts thereof in aqueous solution to obtain an aqueous homogenate; Adding (a) a sugar or sugar alcohol and (b) an amino acid as a stabilizer; and Lyophilizing the aqueous homogenate or a DAO-containing fraction thereof in the presence of the stabilizer. Also disclosed is a lyophilisate obtainable by this method, as well as a solid pharmaceutical dosage form for oral administration containing this lyophilisate. This dosage form is intended for therapeutic use, preferably in the treatment of histamine-induced disorders, or can be used as a dietary supplement or dietary food.

The present invention discloses a neurotransmitter detection method based on an integrated derivatization and solid phase extraction continuous reaction platform; the neurotransmitters include at least: Class A neurotransmitters have no primary amine groups, hydroxyl groups, and carboxyl groups, and have secondary amine groups or quaternary ammonium groups; Class B neurotransmitters contain at least one of primary amine groups, hydroxyl groups, and carboxyl groups; before detection, an aqueous phase derivatization system containing benzoyl chloride is used to derivatize the neurotransmitters in the sample, and an aqueous formic acid solution is added to the obtained supernatant to mix and centrifuge, and the obtained supernatant is subjected to LC-MS analysis after solid phase extraction. The present invention forms an integrated derivatization and solid phase extraction continuous reaction platform through process design, and realizes high-sensitivity simultaneous detection of at least two types of neurotransmitters. Melatonin, serotonin, histamine, and dopamine are detected simultaneously with high sensitivity, and the detection sensitivity is 1pg / mL for melatonin, 1ng / mL for serotonin, 0.03ng / mL for histamine, and 10pg / mL for dopamine.

The invention relates to the technical field of histamine extraction, in particular to histamine removal and extraction equipment and method for apramycin sulfate, the histamine removal and extraction equipment comprises a drying box with a feeding port and a discharging port, an opening and closing unit used for controlling opening and closing of the discharging port is arranged at the discharging port, and a plurality of heating plates sequentially arranged in the horizontal direction are arranged in the drying box; a heating cavity is defined by every two adjacent heating plates; a sliding plate and a driving unit used for driving the sliding plate to move in the length direction of the heating cavity are arranged in the drying box, a plurality of air injection units and a plurality of vertically-arranged spiral conveying units are arranged on the sliding plate, and every two sets of air injection units correspond to one heating cavity and vertically blow air to the cavity walls of the left side and the right side of the heating cavity correspondingly. And the spiral conveying units are in one-to-one correspondence with the heating cavities and extend into the corresponding heating cavities. The drying efficiency of the crystals can be improved.