83 results about "Histamine" patented technology

The invention relates to an SERS (Surface Enhanced Raman Scattering) active nano composite material for detecting trace histamine in tears, SERS sensing test paper as well as a preparation method and application, and belongs to the field of biosensing and spectral analysis. At least one of the problems of low detection sensitivity, poor anti-pollution capacity, low selectivity, incapability of realizing on-site rapid and instant analysis and the like of trace histamine in tears in the prior art is solved. The SERS active nano composite material is of a core-shell structure, and comprises a noble metal nano particle core, the surface of the core is coated with the hollow zeolite imidazate skeleton-67 shell layer, namely a ZIF-67 shell layer of a hollow structure; the Raman reporter molecule is fixed on the surface of the noble metal nanoparticle core. According to the invention, the detection sensitivity, anti-pollution capability and selectivity of trace histamine in tears are improved, and non-invasive rapid detection is realized.

This invention relates to the crystal form of vonorazine pyroglutamate and its preparation method. The vonorazine pyroglutamate crystal form obtained by this invention not only has good solubility but also excellent storage stability; in vitro activity experiments show that crystal form I of this invention is effective against H+. + K + The inhibitory effect of ATPase is comparable to that of TAK-438; animal experiments show that the crystal form of the present invention, when administered intravenously, can significantly inhibit histamine-induced gastric acid secretion in rats, and can exert its pharmacological effect faster than that of TAK-438 administered duodenally.

The invention belongs to the technical field of food safety detection, and particularly relates to a histamine molecular imprinting electrochemical sensor based on a carbon-based nano composite material and a preparation method and application of the histamine molecular imprinting electrochemical sensor. Aiming at the problems of poor selectivity, high detection limit or complicated operation and the like of the existing histamine detection technology, a Co3O4 / N-HCS (cobaltosic oxide / nitrogen-doped hollow carbon sphere) composite material is prepared by adopting a solvothermal method, o-phenylenediamine (o-PD) is taken as a functional monomer, a molecularly imprinted polymer membrane (MIP) is constructed on the surface of a glassy carbon electrode (GCE) by adopting an electropolymerization method, and the histamine detection electrode is prepared. The MIP / Co3O4 / N-HCS / GCE sensor with specific recognition sites on histamine is formed. The sensor has high selectivity, high sensitivity and good stability, is simple and convenient to operate and rapid in detection, can efficiently realize accurate detection of histamine in food, and is suitable for safety monitoring scenes of various foods such as fermented food, aquatic products and dairy products.

The invention discloses a near-infrared ratio type fluorescent probe for rapidly and reversibly detecting histamine (HA), a preparation method of the fluorescent probe and application of the fluorescent probe in dynamic cell imaging, and belongs to the technical field of fluorescence imaging and biosensing. The preparation method comprises the following specific steps: synthesizing aza-BODIPY molecules (1a-1d, 2a-2d) with different halogenations, and meanwhile, connecting the aza-BODIPY molecules with hydroxyl groups at 3 and 5 sites with a hydrophilic PEG (Polyethylene Glycol) fragment and halogen to obtain an HA probe HAPCyto with good water solubility; in addition, the hydroxyl aza-BODIPY molecules are continuously subjected to substitution, halogenation and hydrolysis reaction to obtain carboxyl-containing aza-BODIPY molecules, then the aza-BODIPY molecules are subjected to amidation reaction by utilizing the site, and different organic fragments are respectively connected to obtain a cell membrane targeted HA probe HAPPM, a golgi apparatus targeted HA probe HAPGA and a HaloTag HA fluorescent probe HAPHaloTag. When HA exists in a solution, the developed probe can realize rapid reversible detection of the near-infrared ratio of histamine, also shows good reversible property in mast cells, and can realize dynamic monitoring of histamine in cells.

[Problem] To provide a substance having an activity of antagonizing the binding of histamine to histamine-H3 receptor, or having an activity of inhibiting the homeostatic activity of histamine-H3 receptor. [Means for Solution] A compound of the following formula (I) is provided: [wherein X, Y, Z and W each independently represent a methine group optionally having 1 or 2 substituents, or a nitrogen atom (provided that a case where all of X, Y, Z and W are an optionallysubstituted methine group); A, B and D represent C(0)-, etc.; Q represents a methine group or a nitrogen atom; R represents an optionallysubstituted group of the following formula (II-1): (wherein R7 and R8 each independently represent a lower alkyl group, etc.)].

The application discloses a kind of composite plant extract and its application, skin external preparation.The raw material of the composite plant extract includes hemerocallis fulva flower and red clover, and the weight ratio of hemerocallis fulva flower and red clover is (1:5)~(5:1).The plant extract of hemerocallis fulva flower and red clover of the application can inhibit the release of histamine, thereby reducing the occurrence of inflammation and infection;The plant extract of the application cooperates synergistically, and compared with each plant alone, the histamine inhibition effect of the plant extract of the application is significantly improved;The plant extract of the application uses pure natural plant as raw material, and has low side effect and high safety;The plant raw material used in the application is widely planted in China, and the resource is easy to obtain, the price is cheap, and the cost is low.

An antihistamine compound having the structure of formula (I), a pharmaceutically acceptable salt thereof, a method for preparing the same, and use of the antihistamine compound having the structure of formula (I) and a pharmaceutically acceptable salt thereof in the treatment of diseases such as seasonal / perennial allergic rhinitis, allergic conjunctivitis, and urticaria. JPEG2025519827000061.jpg85170

This invention provides an oligopeptide cyclic peptide and its applications. The cyclic peptide is obtained by truncating the linear Hst1 sequence to the shortest possible sequence that retains Hst1 activity. Then, a triazole ring-containing oligopeptide is prepared by a cycloaddition reaction under specific conditions targeting this shortest sequence. Its molecular weight is only 1 / 3 that of linear histamine, and its biological activity is 15 times that of linear histamine. To achieve the same activity, the cost of the oligopeptide cyclic peptide is only 1 / 100 that of linear histamine, and the yield is significantly higher than that of cyclic peptides directly cyclized with amide bonds. It also exhibits significantly improved stability and resistance to biodegradation. The entire cyclization process of this oligopeptide cyclic peptide is mild, simple, and efficient, and can produce high-purity oligopeptide cyclic peptides. It can be used to prepare anti-aging and repairing skincare products, health products, or pharmaceuticals, and has excellent prospects for large-scale industrialization.

The invention discloses a high-performance biosensor based on a g-C3N4 / TiO2 heterojunction as well as a preparation method and application of the high-performance biosensor, and belongs to the technical field of biosensing and food safety detection. The sensor is constructed by taking a glassy carbon electrode as a substrate and sequentially modifying a g-C3N4 / TiO2 heterojunction composite material and diamine oxidase (DAO). The g-C3N4 / TiO2 heterojunction is prepared through a one-step calcination method, a type II p-n junction structure is formed, photo-generated charge separation and interface electron transfer are effectively promoted, and the conductivity, catalytic activity and enzyme immobilization capacity of the electrode are remarkably improved. The invention discloses a synergistic mechanism of heterojunction interface enhanced histamine selective adsorption and catalytic reaction through density functional theory calculation. The sensor shows excellent detection performance on histamine: the linearity is good in a range of 0.05-10 mM, and the detection limit is as low as 0.075 M; the selectivity is still higher than 93% in the presence of an interferent with ten times of concentration; when the method is applied to detection of actual samples of beef, mutton, fish and chicken, the adding standard recovery rate is 93.60%-101.8%, and the relative standard deviation is smaller than 5%. The sensor has the advantages of high sensitivity, good selectivity, strong anti-interference capability, good reproducibility and the like, and a reliable new method is provided for rapid and accurate screening of histamine in food.

The present application provides a rapid detection system for allergic conjunctivitis and its application. Specifically, the present application provides a rapid detection system for detecting allergic conjunctivitis, which comprises: (A) a subject user terminal; and (B) a background processing end. The rapid detection system of the present application can timely, efficiently and accurately evaluate the incidence and severity of allergic conjunctivitis based on the detection results of the levels of IgE and histamine in tears, realize the home detection of allergic conjunctivitis, reduce misdiagnosis and avoid the aggravation of allergic conjunctivitis due to medical delay, which is helpful to improve the medical efficiency and has a broad clinical application prospect.

The application provides application of an adenosine A2B receptor as a target in a medicine for preventing and / or treating itch, and belongs to the technical field of biological medicines. Based on previously reported single-cell RNA sequencing data of a mouse dorsal root ganglion (DRG), expression of genes encoding main ligand ion channels is detected, Adora2b highly expressed in Neuron_10 is detected, and it is indicated that Adora2b and a receptor (adenosine A2B receptor) of Adora2b can participate in regulating itch. Experiments prove that the activity of the adenosine A2B receptor can be effectively blocked to prevent a histamine-induced scratching behavior of a mouse, and it is indicated that the A2B receptor participates in histamine-induced itch sensation. The application also provides application of a reagent for inhibiting biological functions of the adenosine A2B receptor in preparation of a medicine for preventing and / or treating itch, and provides a new means for prevention and treatment of itch.

The invention discloses a dual-mode sensor based on a histamine aptamer H32 and application of the dual-mode sensor, and belongs to the technical field of food safety analysis and detection. The aptamer as shown in SEQ ID NO.1 is used as a recognition molecule of histamine, the stability is high, the preparation cost is low, and the aptamer has high affinity and high selectivity to histamine. According to the invention, dual-mode signal complementation is realized, ultraviolet and fluorescent dual channels cover a wide dynamic range of 3-30 [mu] M, the detection range is expanded compared with a single-mode detection range, and detection can be rapidly completed. The construction of the sensor provides a choice for aquatic product safety monitoring, and has certain application potential in the fields of aquatic product monitoring and food quality control.

The invention relates to the technical field of biological medicines, and particularly discloses an egg yolk antibody for resisting canine interleukin as well as a preparation method and application of the egg yolk antibody. The egg yolk antibody is prepared by taking immunocompetence antigen epitope peptide of canine interleukin-31 as an immunogen, the antigen epitope peptide is obtained by predicting key antigen epitopes of IL-31 through an Antigenic tool and performing series optimization, a coding gene of the antigen epitope peptide is optimized by an escherichia coli codon to construct a recombinant expression vector, and high-purity antigen is obtained by inducible expression and purification. A laying hen is immunized by the antigen, after basic immunization and two times of booster immunization, the egg yolk antibody is extracted from egg yolk and purified to obtain the egg yolk antibody, and the ELISA of the egg yolk antibody is obviously superior to that of an antibody prepared by commercial canine IL-31 protein immunization. The egg yolk antibody can be specifically combined with canine IL-31, the histamine level in serum of a canine with pruritus is remarkably reduced, the atopic dermatitis related pruritus symptoms are effectively relieved, an efficient and safe biological treatment preparation is provided for canine IL-31 mediated pruritus diseases, and the egg yolk antibody has wide clinical application prospects.

This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.

This invention discloses a molecularly imprinted paper-based microfluidic colorimetric sensor for detecting histamine, its preparation, and its application. The colorimetric sensor (MIP@MIL) is polymerized from a metal-organic framework (MIL-101) using surface molecular imprinting and dual-monomer imprinting techniques. MIP@MIL exhibits a histamine detection limit of 0.77 μM and a detection time of 12.5 min, demonstrating good selectivity. The mechanism involves histamine blocking the imprinted pores of MIP@MIL and acting on MIL-101, thereby weakening the catalytic oxidation process of the chromogenic agent 2,2'-azido-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Furthermore, histamine increases the repulsive force of MIP@MIL against ABTS, thus enabling colorimetric detection of histamine. To further improve practical applicability, MIP@MIL was loaded onto qualitative filter paper and further modified onto microfluidic paper-based chips (μPADs). Using a smartphone, rapid and accurate visual detection of histamine in food was achieved, demonstrating its potential for food freshness detection.

The invention provides a preparation process of an anti-allergic health food, and relates to the technical field of health food preparation. The production process of the anti-allergic health-care food comprises the following steps: preparing the following raw materials: herba cistanche, radix astragali, rhizoma polygonati, a bupleurum chinense leaf extract, a dandelion extract, honey and potassium sorbate, wherein the addition ratio of the bupleurum chinense leaf extract to the dandelion extract is 1: 2; cistanche deserticola, astragalus membranaceus and rhizoma polygonati are mixed, excessive water is added for soaking, reflux extraction and filtration are performed, filtrate is combined, and an extracting solution is obtained. By adding the bupleurum chinense leaf extract and the dandelion extract into an extracting solution of traditional Chinese medicinal materials such as astragalus membranaceus, the ratio of IgG1 / IgG2a can be effectively reduced, namely Th1 type immune response is relatively enhanced, Th2 type response is relatively weakened, allergen-specific IgE antibodies generated by B cells are correspondingly reduced, degranulation reaction and release of inflammatory mediators such as histamine are relieved, and the anti-inflammatory effect is achieved. The anaphylactic reaction is effectively relieved.

The invention relates to SERS (Surface Enhanced Raman Scattering) sensing test paper for detecting trace target molecules in tears as well as a preparation method and a detection method and belongs to the field of biosensing and spectral analysis. At least one of the problems of insufficient detection sensitivity, poor anti-pollution capability, low selectivity, incapability of rapid and on-site instant detection and the like in the existing tear biomarker detection technology is solved. The SERS sensing test paper comprises a porous fiber matrix and an SERS active nano composite material loaded on the porous fiber matrix, the SERS active nano composite material is precious metal nano particles (at) HZIF modified by Raman reporter molecules, and the SERS active nano composite material is of a core-shell structure. According to the invention, ultrasensitive, anti-pollution and rapid on-site detection of trace target molecules (such as histamine) in tears is realized.

The invention relates to the technical field of medicines, in particular to application of ginseng polysaccharide in preparation of a medicine for improving allergy caused by gluten intake. Experimental verification shows that the condition of allergy caused by gluten intake of mice can be remarkably improved by performing drug administration (ginseng polysaccharide) intervention on a gluten allergy mouse model, which is specifically reflected in that the effect of improving allergy caused by gluten intake is achieved by reducing the levels of IGE, IGA, IGG, histamine, IL-4, IL-5, IL-6, LPS and FITC-glucan in serum and improving the levels of IL-2 and INF-gamma. Experimental results show that the ginseng polysaccharide can improve allergy caused by gluten intake, and a new technical thought is provided for preparing products for treating allergy caused by gluten intake.

The application provides an enzymatic preparation method of histamine dihydrochloride, and belongs to the technical field of biochemistry. The method uses L-histidine as a substrate, uses a histidine decarboxylase mutant as a biological catalyst, and converts the substrate into histamine under suitable enzyme catalytic reaction conditions. Finally, the histamine dihydrochloride is obtained through a proper extraction and purification process. The substrate conversion rate of the enzyme catalytic reaction is more than 99%, and the purity of the product after extraction is greater than 98%. The enzyme catalytic method has the advantages of high conversion rate, short conversion time, easy extraction and purification, and great industrialization prospect. The method has the advantages of high conversion rate and high yield, and the obtained product has market competitive advantage.

The present invention relates to a cosmetic composition comprising a combination of nerve growth factor (NGF) inhibitors, that is particularly of use in the topical treatment of pruritus, in particular, that observed in the case of psoriasis or of atopic dermatitis, of chronic urticaria and also of senile pruritus. The invention is directed towards a composition comprising an ambora extract and a green tea extract.

Provided is a means for shortening the onset time of the action of a local anesthetic, an antihistamine, or the like in the oral mucosa. The micro-protrusion patch comprises a base plate and micro-protrusions erected on the base plate, and the micro-protrusion patch is made of water-insoluble thermoplastic polymer materials and used for being applied to oral mucosa. And a topical application system comprising the microprotrusion patch, a coating drug (a gel or ointment containing the drug, etc.), and an adhesive tape for backing, for shortening the action time of the drug in the oral mucosa.

Provided is a means for shortening the time of the development of the action of a local anesthetic agent, an antihistamine drug or the like against skin and oral mucosa. Provided are: a minute projection patch which is intended to be applied to skin or oral mucosa and includes a substrate and minute projections stereoscopically erected on the substrate, in which each of the substrate and the minute projections is made of a water-insoluble thermoplastic polymer substance having an elastic modulus of 1.5×104 kgf / cm2 or less; and a local application system for shortening the time of the action of a drug against the skin or oral mucosa, which contains the minute projection patch and a liniment (a gel, an ointment or the like containing a drug).

This invention discloses an antipruritic gel containing 3-hydroxydecanoic acid microemulsion and its preparation method. The gel combines the natural antipruritic ingredient 3-hydroxydecanoic acid and peppermint oil. By preparing it into a microemulsion form, nano-sized droplets are combined with skin absorption enhancers and gel sustained-release agents, thereby improving transdermal absorption efficiency and prolonging the residence time in the skin to achieve a long-lasting antipruritic effect. 3-hydroxydecanoic acid exerts its antipruritic effect through anti-inflammatory action, blocking pruritus signaling pathways, and reducing mast cell degranulation. This antipruritic gel is stable at room temperature, easy to use, and suitable for relieving skin itching caused by mosquito bites, histamine, or non-histamine substances.

The application discloses a salt-tolerant flavor-enhancing biological amine degradation strain Staphylococcus carnosus WQ-4 and application thereof, and belongs to the technical field of biological fermentation. The Staphylococcus carnosus WQ-4 has been preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251802. The strain has good salt tolerance and biological amine degradation capacity, has obvious degradation effect on histamine and tyramine which are common biological amines in fermented food, the degradation rates are 55.12% and 81.47% respectively, and has good stability, safety and flavor-enhancing effect in a soy sauce fermentation environment, and can be used for soy sauce brewing.

The invention discloses an in-vitro detection method for histamine modification of heart tissue collagen, and belongs to the technical field of biological detection. The in-vitro detection method comprises the following steps: extracting crude collagen in mouse heart tissue after acute myocardial infarction, namely cutting the mouse heart tissue into pieces, cleaning, homogenizing, centrifuging, cleaning the collected precipitate, degreasing with ethanol, and filtering; then shaking in an acetic acid solution containing pepsin to extract collagen, precipitating collagen, dissolving in distilled water, neutralizing, incubating overnight, centrifuging, and re-dissolving collagen precipitate in the acetic acid solution to obtain a collagen solution; carrying out SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and Coomassie brilliant blue dyeing, and separating collagen alpha, beta and gamma chains; meanwhile, the collagen freeze-dried product is subjected to LC-MS / MS detection. According to the present invention, the I-type collagen of the mouse heart tissue after the acute myocardial infarction injury is extracted, and the histamine modification detection is performed on the I-type collagen, such that the technical basis is provided for the acute myocardial infarction treatment.

The invention discloses histamine oxidase protein and application thereof. The amino acid sequence of the histamine oxidase protein is shown as SEQ ID NO. 1. The histamine oxidase protein PAO can be used for preparing the medicine for treating allergic rhinitis; according to the invention, histamine is locally degraded through nasal mucosa, so that local histamine aggregation of nasal mucosa is reduced, symptoms such as sneezing and the like of allergic rhinitis are improved, and nasal 2-type inflammatory response is relieved, so that a treatment effect on allergic rhinitis is achieved, and a brand-new view angle and target spot are provided for treatment of allergic rhinitis.

The application provides a mycoplasma hyopneumoniae culture medium capable of significantly improving the strain titer and an application thereof, and belongs to the field of veterinary biological products. The mycoplasma hyopneumoniae culture medium is obtained by adding L-asparagine, myo-inositol, arachidonic acid, urea, histamine, sodium pyruvate, valproic acid, glyceric acid, lysophosphatidic acid, L-citrulline, L-glutamine and L-leucine into a KM2 culture medium containing Bama pig serum. The culture medium can significantly improve the growth rate and viable bacterial titer of the mycoplasma hyopneumoniae under a low serum concentration, and reduces the production cost.

The invention relates to the technical field of organic electrochemical synthesis, and particularly discloses a method for generating histamine through decarboxylation hydrogenation by taking histidine as a raw material through an electrochemical decarboxylation coupling strategy. According to the method, amino-protected histidine is taken as a substrate, in an alkaline system in which electrolyte exists, a specific electrode material is selected and the current intensity is regulated and controlled, so that after directional removal of carboxyl in histidine molecules is realized, only simple protective group removal treatment is needed, and histamine can be efficiently generated. The method does not depend on biological enzyme catalysis, realizes chemical selective conversion through an electrochemical means, has the advantages of mild reaction conditions, simplicity and convenience in operation, environmental friendliness and the like, and has extremely high industrial production potential.

The invention discloses a method for reducing the content of biogenic amines in meat products through composite vacuum rapid pickling, and belongs to the technical field of food processing. According to the method disclosed by the invention, the pork tenderloin, the chicken breast and the beef tendon are pickled by adopting the pickling liquid prepared by compounding the anthocyanin and the lipopeptide and assisting ultrasonic and vacuum modes, so that the pickling time of the meat product is shortened, the quality of the meat product is improved, the generation of biogenic amine is also inhibited, histamine and tyramine are not detected in the pork tenderloin pickled by the method, and the pork tenderloin is free of toxic and side effects. The content of cadaverine and spermidine is remarkably reduced, and the product is uniform in meat quality, excellent in flavor and high in safety. The method provides a theoretical basis for controlling the safety of meat products and optimizing the processing technology while improving the meat pickling efficiency, and has a key guiding significance for developing low-salt and low-biogenic-amine meat products.