2 results about "Laboratory methods" patented technology

The invention provides dewaxing, repairing and blocking integrated liquid and application thereof in a pathological specimen immunohistochemical operation method. The dewaxing, repairing and blocking integrated liquid is prepared from the following components in percentage by mass: 1.0 to 5.0 percent of Triton-100, 1.0 to 5.0 percent of Tween 20, 0.1 to 3.0 percent of fatty acid methyl ester ethoxylate, 0.1 to 1.0 percent of tetrahydrofurfuryl alcohol, 0.001 to 0.01 percent of 2-methyl-3-isothiazolinone, 0.01 to 4.0 percent of buffer solution and the balance of water. In the process of applying the dewaxing, repairing and blocking integrated body fluid to the pathological specimen immunohistochemical operation method, compared with an existing laboratory method, the dewaxing, repairing and blocking integrated body fluid is used at the temperature of 65-75 DEG C, the steps of xylene dewaxing and gradient alcohol hydration after dewaxing in an existing laboratory can be replaced, the temperature continues to rise to 95-99 DEG C, and when antigen repairing is conducted on a sample, the temperature of the sample is lowered to 95-99 DEG C; according to the method, the step of blocking 0.3-3% of hydrogen peroxide in an existing laboratory is replaced by the enzyme activity of high temperature on endogenous catalase, and the same effects of dewaxing, repairing and blocking in the existing laboratory are achieved on the basis of reducing the experimental steps and the experimental time.

This invention relates to the field of surface-enhanced Raman scattering (SERS), specifically to a surface-enhanced Raman spectroscopy (SERS) method, system, and nanotag preparation method for IL-6 immunoassay. Applied to data analysis in low antigen concentration scenarios, the Raman scan image is first converted into a binary image according to a set threshold, and quantification is performed using the percentage of positive regions (RPP). The exponential relationship between the RPP and antigen concentration verifies that single-molecule events follow a Poisson distribution. Finally, the linear relationship between the product of the RPP and the average Raman intensity of the positive regions (RPP*ARI) and the concentration is used to analyze antigen concentration data. When testing clinical serum samples, this immunoassay method shows strong consistency with standard clinical laboratory methods. The nanotags prepared in this application exhibit clear signals at single nanoparticle resolution, thereby achieving ultrasensitive / single-molecule detection.