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Polymerase Chain Reaction (PCR) PCR is a laboratory method used for making a very large number of copies of short sections of DNA from a very small sample of genetic material. This process is called "amplifying" the DNA and it enables specific genes of interest to be detected or measured.
Surface-enhanced Raman spectroscopy IL-6 immunoassay method, system and nanotag preparation method
ActiveCN119574883BStrong consistencyAchieve ultra-sensitive/single-molecular detectionRaman scatteringBiological testingAntigenImmune profiling
This invention relates to the field of surface-enhanced Raman scattering (SERS), specifically to a surface-enhanced Raman spectroscopy (SERS) method, system, and nanotag preparation method for IL-6 immunoassay. Applied to data analysis in low antigen concentration scenarios, the Raman scan image is first converted into a binary image according to a set threshold, and quantification is performed using the percentage of positive regions (RPP). The exponential relationship between the RPP and antigen concentration verifies that single-molecule events follow a Poisson distribution. Finally, the linear relationship between the product of the RPP and the average Raman intensity of the positive regions (RPP*ARI) and the concentration is used to analyze antigen concentration data. When testing clinical serum samples, this immunoassay method shows strong consistency with standard clinical laboratory methods. The nanotags prepared in this application exhibit clear signals at single nanoparticle resolution, thereby achieving ultrasensitive / single-molecule detection.
Owner:SHENZHEN RESEARCH INSTITUTE OF SOUTHEAST UNIVERSITY +1