55 results about "Laboratory methods" patented technology

The invention provides a method for predicting discharge capacity of a lithium ion battery through partial discharge process by a BP neural network. The terminal voltage of the lithium ion battery in the at least previous 10 minute constant-current discharge process is taken as input, and a BP neural network model outputs the discharge capacity of the battery. The method solves the technical problems of long test period and high energy consumption in the conventional industrial method, also overcomes the defect that a laboratory method has complicated steps and is not suitable for massive industrial production, and guarantees that the average forecasting error is about 2.0 percent which is less than the error range of about 5percent allowable in the industrial production.

A method for measuring of histamine in an epidermis comprising applying an adhesive article to an epithelium of a mammal; allowing for adherence of epithelial cells to the adhesive article; removing the adhesive article from the epithelium of the mammal; preparing the adhesive article using standard laboratory methods for extraction; extracting histamine from the epithelial cells adhered to said adhesive article; measuring histamine from the epithelial cells adhered to said adhesive article; determining the amount of histamine in the epithelial cells as compared to a baseline sample. Further, a method of objectively measuring the perception of itch in mammals and wherein there is a reduction in histamine from a baseline level which is directly proportional to a reduction in an itch perception.

It has been found that a urine test for ovarian reserve can be provided which is equivalent in sensitivity to current laboratory methods which determine ovarian reserve based on FSH levels in serum. A lateral flow device for indicating ovarian reserve is disclosed. The device has an application zone for receiving a urine sample, a labelling zone containing label which binds to FSH in the sample, and a detection zone where FSH-bound label is retained. The detection zone has a threshold value which corresponds to a serum FSII level of between 5-20 mIU / ml (e.g. 10 mIU / ml). A test result above the threshold value indicates diminished ovarian reserve.

The invention relates to the field of transgenic plant detection, in particular to a method for quickly detecting transgenic corn. The method detects glufosinate-resistant Bar genes in the transgenic corn by using loop-mediated isothermal amplification (LAMP). The method does not need special instruments (such as PCR instrument), and is quicker and more economic compared with a conventional laboratory method. The method is simple and convenient in identification, has extremely high specificity, and can judge whether a destination fragment is amplified only by observing with naked eyes or detecting the precipitation turbidity with an ultraviolet imaging system of a turbidity meter so as to judge whether a genome of a core plant sample for detecting contains the glufosinate-resistant Bar genes, and a gel electrophoresis method for observation is not needed.

The invention discloses an enthalpy difference laboratory based on temperature and humidity independent controlling. The enthalpy difference laboratory is internally provided with a humidity adjusting system and a temperature adjusting system which are mutually independently controlled. The humidity adjusting system comprises a solution humidity adjusting air conditioner unit, and the temperature adjusting system comprises a heater. The solution humidity adjusting air conditioner unit and the heater are connected with a computer, and the computer is further connected with a temperature sensor and a humidity sensor which are arranged in the enthalpy difference laboratory. Compared with a method for adjusting the indoor temperature and humidity of a traditional enthalpy difference laboratory, the enthalpy difference laboratory based on temperature and humidity independent controlling has the advantage that the energy-saving effect is remarkably achieved, unnecessary energy source waste caused by cold and hot counteracting is omitted, the control logistic and the control process are simpler, and the adjusting speed is high.

A canister for measuring the natural gas content of rock cores which (1) has an inner core-containment bag made of non-permeable plastic which can be flash evacuated, collapsing around the core and thus minimizing the amount of air present and improving the quality of the gas analyses and (2) which prevents the released gas from reacting with the outer canister. A procedure for using gas-sampling bags to periodically collect the gas released from the core so that (1) the volume of released gas can be measured at a later date using more convenient and precise laboratory methods, (2) the gas can be readily transported and stored, and (3) the gas can be easily submitted for analysis.

The invention discloses a drip type soil infiltration measuring device which comprises a water supplying device, a water drop generating device, a water-collecting measuring device and a bracket system. According to the drip type soil infiltration measuring device, by using a method of drip at a certain height, water drops beat a soil surface to cause that soil generates crust, soil particles absorb water to expand and fill in holes, and soil aggregates are crushed to fill large holes; through regulating different water heads, the speed of drip is controlled, the change similar to waterfall intensity is simulated, different rainfall pattern characteristics are reflected; water collection can be discharged, and thin-layer hydrops exist on the soil surface so that an infiltration environment is close to an infiltration environment under a rainfall condition; the soil infiltration measurement is suitable for laboratory operation, and is closer to soil infiltration under the natural rainfall condition in comparison with a conventional fixed water head laboratory method, and the cost is lower and the simplicity is achieved in operation.

The disclosure relates to methods for determining imbibition of hydraulic fracturing fluids into hydrocarbon-bearing formations. More specifically, the disclosure relates to laboratory methods for determining certain unconventional flow parameters to measure the imbibition over time of hydraulic fracturing fluids into a low-permeability hydrocarbon-bearing rock formation.

The invention discloses a practical method for determining the dredging depth of polluted bottom mud in environmental dredging engineering, which belongs to the technical field of water pollution control and ecosystem restoration. The method comprises the following steps of: sampling columnar bottom mud by combining sensory organ judgment with laboratory analysis; laminating in field and sampling according to physical characteristics; initially determining the dredging depth of polluted bottom mud; further analyzing the pollutant concentration by adopting a laboratory method; hierarchically sorting laboratory data according to indexes; and finally determining the dredging depth of the polluted bottom mud. The method is easy to operate, has low cost, certain accuracy and precision, a wide application range and small quantity of limitation factors, is suitable for large-scale surveying, sampling and analyzing of bottom mud, is a method with high operability and feasibility in determination of an engineering dredging range and dredging depth in polluted bottom mud dredging engineering, and has a wide application prospect in the engineering field.

The invention belongs to the field of preparation of inorganic materials, and particularly relates to a microwave homogeneous hydrothermal synthesis method of magnesium-aluminum hydrotalcite. The method comprises the following steps: weighing soluble magnesium nitrate, aluminum nitrate and urea according to a certain ratio, and dissolving in deionized water; subpackaging the solution in a microwave digestion tank, sealing, putting in a microwave digestion instrument, and performing microwave heating at a set temperature for a set time to quickly finish synthesis; and pouring out all solids and liquids in the microwave tank, washing with deionized water and anhydrous ethanol, performing vacuum drying, grinding, and storing. By adopting microwave radiation heating, the method provided by the invention has the characteristics of energy saving and high efficiency, can realize temperature and pressure on-line control, and has the advantages of high reproducibility and operational safety; meanwhile, the method does not need pH regulation, and is simple to operate; and the product is high in particle size uniformity. In general, the microwave homogeneous hydrothermal synthesis method established by the invention has the characteristic of being simple, quick and efficient to operate, and is a laboratory method for quickly synthesizing magnesium-aluminum hydrotalcite of a uniform particle size.

The invention relates to a real-time gasoline product attribute prediction method based on near infrared spectrum detection. The method comprises the following steps: acquiring a gasoline sample and near infrared spectrum thereof, and measuring the required attribute of the gasoline by using a laboratory method; performing baseline correction on the acquired gasoline spectrum sample by adopting a baseline correction method and selecting proper wavelength range; and finally establishing a mathematic model between an attribute value and the near infrared spectrum for the prediction analysis of the gasoline attribute. The near infrared spectrum of the gasoline sample can be obtained in real time by adopting an online near infrared analyzer; the gasoline product attribute can be predicted in real time, the analysis speed is high, and the signal to noise ratio is high; the method is especially suitable for the online prediction analysis of the gasoline product attribute in a gasoline pipeline blending process.

A novel Fasciclin Related Adhesive Protein (FRAP) from Plasmodium and related parasites is provided as a target for therapeutic intervention in diseases caused by the parasites. FRAP has been shown to play a critical role in adhesion to, or invasion into, host cells by the parasite. Furthermore, FRAP catalyzes the neutralization of heme by the parasite, by promoting its polymerization into hemozoin. This invention provides methods and compositions for therapies based on the administration of protein, DNA or cell-based vaccines and / or antibodies based on FRAP, or antigenic epitopes of FRAP, either alone or in combination with other parasite antigens. Methods for the development of compounds that inhibit the catalytic activity of FRAP, and diagnostic and laboratory methods utilizing FRAP are also provided.

The present invention relates generally to the field of cell culture, which is a laboratory process used primarily for the growth, propagation, and production of cells for analysis and the production and harvesting of cell products. The present invention comprises functionalized and / or engineered hydrogel microcarriers that exhibit any or all of the following properties: controllable buoyancy, ferro- or paramagnetism, molecular or fabricated reporting elements, and optical clarity. The microcarriers are used in a bioreactor that employs external forces to control said microcarrier kinetic energy and translational or positional orientation in order to facilitate cell growth and / or cellular analysis. The bioreactor can be part of an automated system that employs any or all of the following; a microcarrier manufacturing method, a monitoring method, a cell culture method, and an analytical method. Either a single bioreactor or a plurality of bioreactors are used in the automated system to enable cell culture and analysis with a minimum of human intervention.

The disclosure relates to methods for determining imbibition of hydraulic fracturing fluids into hydrocarbon-bearing formations. More specifically, the disclosure relates to laboratory methods for determining certain unconventional flow parameters to measure the imbibition over time of hydraulic fracturing fluids into a low-permeability hydrocarbon-bearing rock formation.

A method for measuring of histamine in an epidermis comprising applying an adhesive article to an epithelium of a mammal; allowing for adherence of epithelial cells to the adhesive article; removing the adhesive article from the epithelium of the mammal; preparing the adhesive article using standard laboratory methods for extraction; extracting histamine from the epithelial cells adhered to said adhesive article; measuring histamine from the epithelial cells adhered to said adhesive article; determining the amount of histamine in the epithelial cells as compared to a baseline sample. Further, a method of objectively measuring the perception of itch in mammals and wherein there is a reduction in histamine from a baseline level which is directly proportional to a reduction in an itch perception.

The invention aims to provide a method for detecting chrysanthemum virus B (CVB) by utilization of loop-mediated isothermal amplification (LAMP). The method utilizes loop-mediated isothermal amplification (LAMP) to detect the chrysanthemum virus B (CVB). The method comprises the steps of preparation of a reaction system, incubation treatment, inactivation treatment and product detection in turn, wherein the reaction system comprises 0.6 to 1.4 mol / L of Betaine, 0.25 to 2.5 mmol / L of dNTP, 2 to 12 mmol / L of Mg<2+>, an outer primer and an inner primer, and the concentration ratio of the inner primer to the outer primer is 1:1-1:8. The method has the advantages that the method does not need a special instrument (such as a PCR instrument), and is quicker and more economic than the prior laboratory method; and the method is simple and convenient to identify, has high specificity, can judge whether a target fragment is amplified or not by observing with the naked eye or detecting the turbidity of deposit through a turbidity meter and an ultraviolet imaging system, so as to judge whether a sample plant to be detected is infected with the chrysanthemum virus B or not, and does not need to use a gel electrophoresis method for observation.

LAT (Linker for Activation of T-cells) is a protein involved in signaling through the T-cell receptor (TCR). The invention provides a LAT protein including mutations at ubiquitylation sites that result in an increase in stability of LAT in stimulated and unstimulated cells, and enhanced signaling through the TCR. The invention further provides use for a LAT protein including mutations at ubiquitylation sites for therapeutic and laboratory methods.

The invention discloses a quick detection and evaluation method of harmful precipitated phase in two-phase stainless steel. The method comprises the following steps: S1, determining harmful phase content in two-phase stainless steel at different sensitization time by using a counting method and a ferrite instrument, and drawing two results as a relation graph; S2, determining the hardness of the two-phase stainless steel with different harmful phase contents, and drawing a relation graph of the harmful phase content and the hardness; S3, respectively performing solution corrosion and electrolytic corrosion on the two-phase stainless steel with different harmful phase contents, and observing the similarity of the metallographic structure of two corrosion methods, thereby acquiring a metallographic atlas; and S4, comparing with a field detection result according to relation graphs and the metallographic atlas, judging whether the harmful phase is existent in the two-phase steel in site,and estimating the volume fraction of the harmful phase. By establishing the correlation between an accurate laboratory method and an operable field method, whether the harmful phase is existent in the two-phase steel base material can be quickly judged in site, the method is simple and practical, and the time and cost for sampling to the laboratory to analyze are saved.

The invention discloses a biosensor for organophosphorus pesticide rapid detection and a detecting method of the biosensor. A saturated calomel electrode is used as a reference electrode; a platinum plate electrode is a counter electrode; a glass carbon electrode fixed with tyrosinase and 1,2-naphthoquinone-4-sodium sulfonate substrate is used as a working electrode; the working electrode is prepared by electrically and chemically polymerizing and fixing the tyrosinase and the 1,2-naphthoquinone-4-sodium sulfonate substrate on the glassy carbon electrode with a non-conducting polymer. Therefore, the biosensor can detect the organophosphorus pesticide more reliably, sensitively and rapidly. The biosensor is simple to prepare, reusable, high in utilization rate, and suitable for field operation, can be used to detect the organophosphorus pesticide without preparation of enzyme and substrate solution every time, can screen out a large number of unqualified samples in short time, shortens the range of quantitative detection, and is an effective supplement for the complicated laboratory method.

A laboratory method and a test specimen for efficiently and accurately assessing and validating the efficacy of an antimicrobial to treat pathogens on a beef carcass. The method and model employ one or more carcasses from calves that have not received colostrum.

A canister for measuring the natural gas content of rock cores which (1) has an inner core-containment bag made of non-permeable plastic which can be flash evacuated, collapsing around the core and thus minimizing the amount of air present and improving the quality of the gas analyses and (2) which prevents the released gas from reacting with the outer canister. A procedure for using gas-sampling bags to periodically collect the gas released from the core so that (1) the volume of released gas can be measured at a later date using more convenient and precise laboratory methods, (2) the gas can be readily transported and stored, and (3) the gas can be easily submitted for analysis.

Method of processing photographs in a photographic laboratory by means of a photographic processing system, comprising the steps of:receiving customer orders and processing information respectively assigned to said customer orders, each customer order comprising at least one photographic image, controlling the processing of a customer order in accordance with an assigned processing information; checking each processing information to ascertain whether or not the processing information may be automatically processed by said processing system and identifying those customer orders which are to be processed according to a non-automatically processable processing information.

The invention discloses a method for making laboratory dumplings and a quality evaluation method thereof. The preparation method comprises the following steps: (1) kneading the dough, the amount of water added during the kneading is: (41-100×flour moisture content)×flour weight / 59; (2) pressing and making dumpling wrappers, the dumpling The thickness of the skin is 1.20mm-1.30mm, and the diameter of the dumpling skin is 7cm-8cm; (3) making dumpling stuffing, the dumpling stuffing is made up of cabbage, scrambled eggs, edible oil, diced green onion, minced fresh ginger and salt; (4) Make dumplings and boil dumplings, add 3g of the dumpling filling to each dumpling skin; boil the dumplings and keep them for 5 minutes to obtain the laboratory dumplings. The method for evaluating the quality of the laboratory dumplings comprises the following steps: performing sensory evaluation on the laboratory dumplings and the reference product dumplings.

The invention relates to a laboratory method for preparing hydrocarbons by deoxidizing stearic acid, belonging to production and preparation of biofuels. The method comprises the following steps: weighing 5 wt.% of Pd / C catalyst, adding the catalyst into a reaction kettle, charging hydrogen gas of which the volume percent is 25%, gradually heating to 200 DEG C, and keeping the temperature for 30 minutes to carry out catalyst pretreatment; and adding the pretreaed Pd / C catalyst, stearic acid and dodecane into the reaction kettle, charging hydrogen gas and argon gas, heating while stirring, and filtering to separate out the Pd / C catalyst, thereby obtaining the catalytic deoxidization reaction products, such as hydrocarbons and the like, wherein the Pd / C catalyst-stearic acid-dodecane mass ratio is (2-5):90:80. The Pd / C catalyst has the advantages of high catalyst activity and stable performance, and can be recycled; and the prepared petroleum-base hydrocarbon liquid fuel has the advantages of high heat value, high stability and high low-temperature fluidity, and thus, has instruction meaning and development value.

The invention relates to a near-infrared measurement method applicable to real-time on-line measuring-point-free temperature compensation. The method comprises the following steps: measuring physical property parameter standard data by utilizing a laboratory method; acquiring the near infrared spectrum of a same sample under different temperature levels; performing preprocessing and principal component analysis on the acquired spectral data, and performing non-separated latent variable modeling on the temperature by using partial least squares so as to acquire a physical property parameter measurement value at the present moment; and acquiring a new spectral data set online, constructing an on-line recursive algorithm, updating the physical property parameter measurement value, and finishing near-infrared on-line measurement capable of achieving a measuring-point-free temperature compensation function. According to the method disclosed by the invention, the temperature serving as a non-separated latent factor variable participates in the near-infrared modeling process, and the recursive algorithm is formed based on the to-be-measured property parameters, so that the property parameter measurement under different temperatures can be finished by virtue of adaptation of the model on the temperature, and direct temperature measurement information and correlation calculation are not needed; and therefore, the established model has the high universality. The on-line recursive algorithm is not sensitive on sample temperature change, and the measurement result has a relatively small integrated error.