349 results about "Paramagnetism" patented technology

The present invention relates generally to the field of cell culture, which is a laboratory process used primarily for the growth, propagation, and production of cells for analysis and the production and harvesting of cell products. The present invention comprises functionalized and/or engineered hydrogel microcarriers that exhibit any or all of the following properties: controllable buoyancy, ferro- or paramagnetism, molecular or fabricated reporting elements, and optical clarity. The microcarriers are used in a bioreactor that employs external forces to control said microcarrier kinetic energy and translational or positional orientation in order to facilitate cell growth and/or cellular analysis. The bioreactor can be part of an automated system that employs any or all of the following; a microcarrier manufacturing method, a monitoring method, a cell culture method, and an analytical method. Either a single bioreactor or a plurality of bioreactors are used in the automated system to enable cell culture and analysis with a minimum of human intervention.

Disclosed is an improvement in methods for nucleic acid and immunological bioassays. The methods comprise a step for “sweeping” paramagnetic bead: target molecule complexes so as to capture them with an affinity capture agent on a test pad by moving a magnetic force field from outside to inside the test pad area so as to bring into contact the paramagnetic complexes with the capture agent, while sweeping any unbound paramagnetic material off the test pad by moving the magnetic field from inside to outside the test pad area. Surprisingly, the paramagnetic complexes are rapidly affinity-extracted from the moving magnetic field.

The invention relates to a multifunctional double-layer core-shell structure magnetic nano particle. In the invention, a magnetic nano particle with a particle size of 1-300 nm is used as a core and coated with a double-layer shell consisting of a SiO2 layer with a thickness of 1-200 nm and a hydrolyzed silane coupling agent layer is 1-100 nm thick and comprises one or more multifunctional groups; the particle size and the shell layer thickness can be controlled through regulating the volumes, the weight ratios and the reaction time of the magnetic core, a silicon dioxide precursor, a silane coupling agent and a catalyst in a preparation process; the total particle size of the nano particle can be as small as 5-50 nm and as large as 700-800 nm; the nano particle can have superparamagnetism, paramagnetism and ferromagnetism according to the change of the magnetic core particle size; and one or more bioactive molecules can be connected into the shell layer of the magnetic nano particle or to the surface of the shell layer through a chemical method or a physical method. The invention also provides a preparation method of the multifunctional double-layer core-shell structure magnetic nano particle and application thereof. The particle preparation method has the advantages of simplicity, moderate condition, low cost and easy realization of industrial production. The nano particle can obtain different functions through connecting different bioactive molecules and can be applied to the fields of protein enrichment, biological detection, separation and purification, targeted drug carriers, cell imaging and medical imaging.

The present invention provides the preparation process of super-paramagnetic polymer microsphere. The super-paramagnetic polymer microsphere consists of polyolefin polymerized with lipophilic olefin monomer and organic monomer with hydrophilic functional radical and coated nano magnetic grains of modified ferroferric oxide. The nano magnetic grains with one surface lipophilic layer can be well dispersed in organic matter to form magnetic fluid and dispersed inside polymer microsphere to obtain super-paramagnetism. The microsphere is regular and has size of 0.1-5 micron and has magnetic ferroferric oxide accounting for 0.5-40 wt% of the whole micro microsphere. The present invention has the advantages of simple preparation process, narrow microsphere size, regular appearance, high functional radical content, homogeneous magnetism, stable chemical performance and acid and alkali resistance.

The invention relates to a synthetic method for a magnetic metal organic framework composite material coated by [Cu2(btc)2] on surfaces of ferroferric oxide microspheres and application of the composite material. The method comprises the following steps of: firstly synthesizing ferroferric oxide microspheres by a hydrothermal synthesis method; dispersing magnetic spheres in an ethanol liquid of mercaptoacetic acid, wherein hydroxyls are formed on the surface of the spheres; dispersing mercaptoacetic acid modified magnetic spheres to an ethanol liquid of copper acetate, reacting for 15 minutes at 70 DEG C, and then dispersing the product in an ethanol liquid of trimesic acid and reacting for 30 minutes at 70 DEG C; and performing alternate reaction of magnetic spheres with copper acetate and the ethanol liquid of trimesic acid to finally, obtain the magnetic metal organic framework composite material with a core-shell structure. The material has a metal organic framework shell layer and can be coordinated with peptide fragments with amino groups and carboxylic group so as to enrich low concentration peptide. Meanwhile, the enriching and separating process is fast, simple and convenient due to high paramagnetism of ferroferric oxide. The synthetic method is simple and low in cost, and can be used for enrichment and separation of low abundance peptide fragments less than 1nM and MALDI-TOFMS (Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometer) detection.

The invention provides superparamagnetic nano Fe3O4 particles synthesized through in-situ induction of chitosan hydrogels, relating to a method for synthesizing the superparamagnetic nano Fe3O4 particles. The invention solves the problems of serious aggregation of the nano Fe3O4 particles and the complicated method for inducing the chitosan into the surface of the Fe3O4 in the current nano Fe3O4 particles. The method of the invention is that: 1. the chitosan powder is added into dilute acid solution; 2. cross-linking agent is added to make the chitosan hydrogels; 3. the chitosan hydrogels are sequentially dipped in Fe3+ aqueous solution, water, Fe2+ aqueous solution and water, and a plurality of times of circular leaching are made so as to form the chitosan hydrogels that contains iron ions; 4. then basification treatment is carried out; 5. the hydrogels is dissolved or degraded again, and finally the black superparamagnetic Fe3O4 nano particles are obtained upon centrifugalization. The method of the invention requires simple technique and mild conditions and the equipment used in the method is simple and can be obtained easily, thereby mass production can be achieved. The average diameter of the particles is 15 to 25nm, and the particles distribute evenly with superparamagnetism.

A magnetic core-shell microparticle with strong response power to magnetic field is composed of SiO2 as shell and 20-1000 nanometre-class magnetic ferrite particles with super-paramagnetism. Its preparing process includes such steps as preparing the said ferrite particles by the deposition method, dispersing them in the aqueous solution of water-soluble inorganic salt containing SiO2, stirring, and hydrolysis reaction of ethyl n-silicate for coating SiO2 again.

The invention relates to a preparation method of a magnetic polymer heavy metal ion adsorbent. An improved chemical co-precipitation method is used for preparing magnetic nano particles, surface modification is conducted on the magnetic nano particles with gamma-choropropyltrimethoxysilane and polyethyleneimine to obtain polyethyleneimine modified magnetic nano particles, and finally, the polyethyleneimine modified magnetic nano particles and functional monomers are subjected to copolymerization to prepare the magnetic polymer heavy metal ion adsorbent with a multi-level cross-linking structure and multiple adsorption groups; the saturation magnetization is 3.5-15.5 emu/g, remanent flux density and coercive field strength both approach to zero, the paramagnetism and the magnetic responsiveness are provided, for a heavy metal ion aqueous solution with an initial concentration of 100-1000 mg/L, the heavy metal ion adsorption capacity reaches 35-450 mg/g, adsorption equilibrium can be reached in 50-100 min, the heavy metal ion adsorption capacity after 5-time recycling is more than 85% of the first adsorption capacity, and the method can be widely applied in heavy metal ion adsorption and separation, pollution abatement of heavy metal ions and the like.

The invention relates to a magnetic flocculant and a preparation method thereof, wherein the magnetic flocculant is cationic polyacrylamide-modified magnetic nanometer Fe3O4 particles. The magnetic flocculant provided by the invention retains the advantages of low dosage of organic high molecular flocculant, high flocculation speed, coexistence of salts, low pH value and temperature influence, low total amount of flocculating constituent and the like; and the superparamagnetism of the flocculant enables the flocculant to quicken the precipitation under the effect of an applied magnetic field and to realize quick and efficient separation of residual flocculant and flocculation. The magnetic flocculant provided by the invention has the advantages of simple preparation technique, low cost, easiness in amplification and no secondary pollution, and facilitates realizing efficient and economic flocculation operation.

A monometer-level microsphere of magnetic polymer is prepared from the hydrophilic monomers (acrylamide, acrylic acid, etc.) through preparing super-paramagnetic nm microspheres of iron oxide by chemical deposition method, adding emulsifier, trigger, cross-linking agent and said iron oxide microspheres, and reaction in redox or thermal triggering system. The resultant microsphere has super paramagnetism and adjustable granularity (70-140 nm).

In producing an oxygen reduction catalyst including a nitrogen-containing active carbide by converting either a mixture of a carbonaceous solid raw material (coal-derived binder pitch) and a nitrogen-containing organic compound (melamine or the like) or a nitrogen-containing organic polymer compound (polyacrylonitrile, melamine resin or the like) into a powdery material, baking the powdery material, and subjecting the baked product to steam activation, the presence ratio of nitrogen and the presence ratio of carbon relating to a shake-up process in the surface and the spin density of unpaired electrons showing Curie paramagnetism are controlled to be high, by selection of the baking temperature, the mixing ratio between the carbonaceous solid raw material and the nitrogen-containing organic compound, or the nitrogen-containing organic polymer compound material. In incorporating the catalyst into an electrochemical device, the catalyst and an ion conductive polymer are mixed and a catalyst layer is formed from the mixture so as to make smooth the movement of ions and electrons, and, in applying the catalyst to a polymer electrolyte type fuel cell, an MEA is produced. This makes it possible to provide a catalyst comprised of a nitrogen-containing active carbide and a production method thereof, and an electrochemical device using the catalyst.

A magnetic recording medium, including a substrate, and a data-storing thin film layer formed over the substrate. The data-storing thin film layer is comprised of cobalt (Co), platinum (Pt), and a multi-component oxide. The multi-component oxide has cations with a reduction potential of less than −0.03 electron volts, and atomic radii of less than 0.25 nanometers. Additionally, the multi-component oxide is diamagnetic, paramagnetic, or magnetic with a permeability of less than 10⁻⁶ m³/kg. The multi-component oxide has a dielectric constant greater than 5.0. The sputter target is further comprised of chromium (Cr) and/or boron (B).

The invention relates to an ultra-paramagnetic material of load CuO, which is composed of ultra-paramagnetic particles, a mesoporous material layer, and CuO loaded on the internal and the external surfaces of mesoporous material layer; the preparation method includes: produce ultra-paramagnetic particle or its infusible precursor with the common sedimentation method; cover a layer of compact SiO2 or molecular sieve on the surface of the ultra-paramagnetic particle or the infusible precursor chemically with the sol-gel method; increase the temperature and maintain the temperature in gas atmosphere; samples obtained are placed into infusible cupric salt solution for separation, washing, filtering, and drying; burn in gas atmosphere; obtain the material with performances such as ultra-paramagnetism, catalyzing, and decoking. On one hand, the invention solves the problem of stability and conglobation of ultra-paramagnetic particle in the using process, and on the other hand, the invention makes it easier to separate catalyzer and desulfurizer in the using process. Meanwhile, the ultra-paramagnetic material of load CuO is also an infrared radiation material or microwave absorption material with high performance.

The invention relates to the field of nano materials and discloses a preparation method of a water-soluble ferroferric oxide magnetic nanoparticle with functional amidogen, comprising the following steps of: coordinating carboxyl on n-(1,3-dioxoisoindolin-2-yl) alkyl acid (n is equal to 3-18) with ferrum; pyrolyzing a precursor of n-(1,3-dioxoisoindolin-2-yl) alkyl acid iron at high temperature to obtain an oil-soluble magnetic nanoparticle with lipophilicity and a surface ligand, i.e. the n-(1,3-dioxoisoindolin-2-yl) alkyl acid; then removing phthalic anhydride for protecting amino by a deammoniation protective reagent (hydrazine hydrate) by using a deammoniation protection method, and converting the oil-soluble magnetic nanoparticle into the water-soluble ferroferric oxide magnetic nanoparticle with functional amidogen. The water-soluble ferroferric oxide magnetic nanoparticle with functional amidogen can be excellently dissolved in a water solution and has favorable biocompatibility and paramagnetism.

The invention provides a method for preparing a nanocomposite material by assembling ferriferrous oxide in a halloysite tube, and belongs to the technical field of deep processing of nonmetallic minerals. The method comprises the following four steps: (1) pretreatment, tube expansion and purification of the halloysite minerals; (2) modification in the halloysite tube; (2) preparation of Fe3O4 magnetic nanoparticles through a mixed hydrothermal solution technology; and (4) vacuum impregnation for obtaining the compoiste material with the nano-Fe3O4 assembled in the halloysite tube. The halloysite/Fe3O4 composite material prepared in the invention has no residual magnetization or coercive force, and has a typical paramagnetic performance. The material prepared through the method has good biocompatibility, and broadens the application prospect of mineral magnetic materials in the fields of biomedicines and composite magnetic materials.

Magnetic-particle photocatalyst with a core-shell structure takes a ferromagnetism particle, a paramagnetism particle or a diamagnetism particle as a core, and photocatalyst as a shell. The ferromagnetism, paramagnetism or diamagnetism particles are gamma-Fe2O3, SiO2 or MnO particle respectively, and the photocatalyst is Gd3-xBixSbO7, Gd3-xYxSbO7 and In3-xBixTaO7 respectively, wherein the grain diameter of the gamma-Fe2O3, SiO2 and MnO is 80-2,000nm. Water-treatment is carried out on the photocatalyst in a magnetic field-photocatalytic system, and the periphery of a reaction system for water-treatment is provided with an alternating magnetic field generator with adjustable magnetic field intensity, wherein the range of the magnetic field intensity is 0-15T, and radiation light sources of the reaction system adopt a 300W xenon lamp and a 400W high-voltage mercury lamp; the ferromagnetism particle, paramagnetism particle and diamagnetism particle respectively account for 33+/-10% in themagnetic-particle core for coating the photocatalyst, and are completely and uniformly dispersed in aqueous solution, thus the composite photocatalyst is uniformly distributed in the upper, middle and lower layers of the aqueous solution.

The invention belongs to the field of a magnetic nano-drug/a genetic carrier, in particular to a temperature-sensitive amphiphilic block copolymer/an iron oxide genetic carrier which has the capacity of controlling and relieving drug, a preparation method thereof and application thereof. Polyethyleneimine and polyoxyethylene-polypropylene oxide-polyoxyethylene amphiphilic block copolymer (Pluronic) are decoratively arranged on the surface of iron oxide super paramagnetic nanometer particles. DNA/RNA and the drugs having groups of negative electricity are loaded by the use of groups of positive electricity that are decoratively arranged on the Pluronic; drug loading and released temperature control can be realized by the temperature sensitivity of Pluronic near the body temperature; the amphiphilicity of Pluronic can used for realizing simultaneous loading of hydrophobic/hydrophilic drugs and improving the penetration capability of a drug carrier; the excellent dispersity of the carrier and the long-acting circulation through the body are realized by the biocompatibility of Pluronic; the super paramagnetism of iron oxide kernel is used for realizing targeting delivery of the drug.

The invention discloses a preparation method and application of a mercaptan fluorescence probe based on coumarin. The preparation method includes: coumarin and p-tert-butylphenol dialdehyde are used as raw materials to synthesize a bis-Schiff base coumarin derivative (compound I), the compound I has strong fluorescence, and the compound I is coordinated with copper ions to prepare coumarin-copper ion (II) complex, namely the mercaptan fluorescence probe I-Cu (II). Due to the influence of the paramagnetism of Cu2+ and photoinduction electron transfer effect, the fluorescence of the compound I is quenched; sulfur atoms in compounds such as mercapto-amino acid and derivatives such as glutathione thereof and Cu2+ have a strong coordination effect, the competitive coordination effect allows the copper ions in the coumarin-copper ion (II) complex to be taken away by the mercapto-amino acid and the derivatives thereof so as to regenerate the compound I, and the fluorescence of the compound I is restored. Accordingly, a method for fast and simply detecting mercaptan compounds is built.

The invention relates to a magnetic bifunctional catalyst, a preparation method therefor and application of the magnetic bifunctional catalyst in a methanol catalyzed reaction and belongs to the technical field of magnetic bifunctional catalysts and fine chemical industry. According to the magnetic bifunctional catalyst, the surface of each magnetic Fe3O4 core is coated with a TiO2 shell layer with a mesoporous structure, and mesopore of each TiO2 shell layer are loaded with catalytic active components such as Pd, Au and Pd-Au. The method comprises the steps: preparing Fe3O4 cores with super paramagnetism; then, coating the surface of each magnetic Fe3O4 core with the TiO2 shell layer with the mesoporous structure by an ultrasonic-hydrothermal method in a manner of taking titanium sulfate, titanyl sulfate and the like as a titanium source and taking polyethylene glycol as a template agent, so as to prepare magnetic TiO2@Fe3O4 nanospheres; and finally, loading the catalytic active components such as Pd, Au and Pd-Au into the mesopore of each TiO2 shell layer. The magnetic bifunctional catalyst has very good methanol conversion ratio and overall selectivity of methylal and methyl formate, and the reacted catalyst can be separated and recovered by an external magnetic field.

A high capacity magnetic filter separates diamagnetic and/or paramagnetic substances from fluid streams. Diamagnetic solid substances are magnetized under an external magnetic field through coordinated interaction of diamagnetic solid substances with an inducement paramagnetic material (IPM). The magnetic filter serves as a separation zone created by the presence of IPM and magnets that are shielded from the IPM by non-magnetic sleeves or partitions. The IPM in the void volume between the magnets affords large surface area onto which diamagnetic and paramagnetic materials can contact and be attracted to. The relative position and distance of the magnetic source, such a magnetic bar or electromagnet, to the solid mixture of diamagnetic and IPM are adjusted to induce sufficiently strong magnetism in the diamagnetic solids which causes the diamagnetic solids to be attracted by the magnetic field as well. Both diamagnetic and paramagnetic substances can be removed from a liquid or gas.