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Temperature sensing parents block polymer/iron oxide magnetic nano-carrier, preparation method and application thereof

A technology of magnetic nano-carriers and block copolymers, which is applied in the direction of pharmaceutical formulations, genetic material components, and medical preparations of non-active ingredients, etc., and can solve problems such as complex preparation processes, few reports, and poor biocompatibility , to achieve the effect of simple synthesis method, sensitive temperature response, and improved penetration ability

Inactive Publication Date: 2008-07-16
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with iron oxide / polymer composite supports, the preparation process of these materials is complicated and their biocompatibility is not good
[0005] Therefore, novel iron oxide / polymer nanocarriers with drug-controlled release capability will be an effective tool for targeted drug and gene therapy, but few reports have been reported so far.

Method used

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  • Temperature sensing parents block polymer/iron oxide magnetic nano-carrier, preparation method and application thereof
  • Temperature sensing parents block polymer/iron oxide magnetic nano-carrier, preparation method and application thereof
  • Temperature sensing parents block polymer/iron oxide magnetic nano-carrier, preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0041] (1) Activate P123 with carbonyldiimidazole in anhydrous acetonitrile, wherein the molar ratio of P123 to carbonyldiimidazole is 5:3, and after stirring for 3 to 4 hours at a temperature of 45° C., the solvent is removed in vacuo; Add polyethyleneimine (2Kda), wherein the mass percentage of polyethyleneimine and P123 is 1:3, the activated P123 reacts with polyethyleneimine molecules in a carbonate buffer solution, and the product is dialyzed in ethanol, The polyethylenimine-modified P123 polymer was obtained and lyophilized for use.

[0042] (2) 4.69g FeCl 3 ·6H 2 O and 1.72 g FeCl 2 4H 2 O was dissolved in a 500mL stirred reactor, and under the protection of nitrogen, the temperature was raised to 80°C, poured into 20mL of concentrated ammonia water, added 153mg of sodium citrate, and reacted for half an hour to obtain superparamagnetic iron oxide nanoparticles rich in carboxyl groups on the surface. The particle diameter is 20-30nm.

[0043] (3) Add 100mL of 0.04g...

Embodiment 2

[0045] (1) Activate P123 with carbonyldiimidazole in anhydrous acetonitrile, wherein the molar ratio of P123 to carbonyldiimidazole is 5:3, and after stirring for 3 to 4 hours at a temperature of 45° C., the solvent is removed in vacuo; Add polyethyleneimine (20Kda), wherein the mass percentage of polyethyleneimine and P123 is 1:3, the activated P123 reacts with polyethyleneimine molecules in a carbonate buffer solution, and the product is dialyzed in ethanol, The polyethylenimine-modified P123 polymer was obtained and lyophilized for use.

[0046] (2) 4.69g FeCl 3 ·6H 2 O and 1.72 g FeCl 2 4H 2 O was dissolved in a 500mL stirred reactor, and under the protection of nitrogen, the temperature was raised to 80°C, poured into 20mL of concentrated ammonia water, added 153mg of sodium citrate, and reacted for half an hour to obtain superparamagnetic iron oxide nanoparticles rich in carboxyl groups on the surface. The particle diameter is 20-30nm.

[0047] (3) 100mL of 0.04g / mL...

Embodiment 3

[0049] (1) Activate P123 with carbonyldiimidazole in anhydrous acetonitrile, wherein the molar ratio of P123 to carbonyldiimidazole is 5:3, and after stirring for 3 to 4 hours at a temperature of 45° C., the solvent is removed in vacuo; Add polyethyleneimine (2Kda), wherein the mass percentage of polyethyleneimine and P123 is 1:3, the activated P123 reacts with polyethyleneimine molecules in a carbonate buffer solution, and the product is dialyzed in ethanol, The polyethylenimine-modified P123 polymer was obtained and lyophilized for use.

[0050] (2) 4.69g FeCl 3 ·6H 2 O and 1.72 g FeCl 2 4H 2 O was dissolved in a 500mL stirred reactor, and under the protection of nitrogen, the temperature was raised to 80°C, poured into 20mL of concentrated ammonia water, added 400mg of sodium citrate, and reacted for half an hour to obtain superparamagnetic iron oxide nanoparticles rich in carboxyl groups on the surface. The diameter of the particles is 5-15nm.

[0051] (3) Add 100mL of ...

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Abstract

The invention belongs to the field of a magnetic nano-drug / a genetic carrier, in particular to a temperature-sensitive amphiphilic block copolymer / an iron oxide genetic carrier which has the capacity of controlling and relieving drug, a preparation method thereof and application thereof. Polyethyleneimine and polyoxyethylene-polypropylene oxide-polyoxyethylene amphiphilic block copolymer (Pluronic) are decoratively arranged on the surface of iron oxide super paramagnetic nanometer particles. DNA / RNA and the drugs having groups of negative electricity are loaded by the use of groups of positive electricity that are decoratively arranged on the Pluronic; drug loading and released temperature control can be realized by the temperature sensitivity of Pluronic near the body temperature; the amphiphilicity of Pluronic can used for realizing simultaneous loading of hydrophobic / hydrophilic drugs and improving the penetration capability of a drug carrier; the excellent dispersity of the carrier and the long-acting circulation through the body are realized by the biocompatibility of Pluronic; the super paramagnetism of iron oxide kernel is used for realizing targeting delivery of the drug.

Description

technical field [0001] The invention belongs to the field of magnetic nano-medicine / gene carrier, and in particular relates to a temperature-sensitive amphiphilic block copolymer / iron oxide magnetic nano-carrier with controlled drug release capability and its preparation method and application. Background technique [0002] The targeted delivery and controlled release of drugs is a research hotspot in modern biomedicine. The gene therapy technology that has emerged in recent years has also put forward high targeting requirements for gene delivery to improve its effectiveness and safety. Magnetic nanocarriers are one of the effective means to achieve the above goals. Under the directional action of an external magnetic field, the magnetic nanocarriers loaded with drugs / genes can be effectively enriched in the desired treatment area, and at the same time the drugs / genes are released to achieve local high concentration and play a role. Therefore, the utilization efficiency of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/34A61K47/04A61K47/48A61K48/00C08G81/02A61K47/10
Inventor 郭晨陈澍刘会洲
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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