206 results about "Paramagnetic particles" patented technology

A Raman spectroscopy technique allows an analyte, a paramagnetic particle, and a spectral enhancement particle to combine in solution and for the combination product to be localized by a magnetic field for analysis. The spectral enhancement particle may be comprised of an active SERS metal particle with or without a material coating. The spectral enhancement particle may function as a reporter for the presence of the analyte or merely increase the magnitude of the Raman spectrum of the analyte. The technique is applicable to both immunoassays and chemical assays. Multiple spectral enhancement particle reporters may be measured in a single assay that can detect multiple analytes using the SERS effect.

A catheter adapted to deliver therapeutic or diagnostic agents to a target tissue of a human body is disclosed. A catheter in accordance with the present invention comprises a magnetic field source for directing the flow of the therapeutic of diagnostic agent. The therapeutic or diagnostic agent may be conjugated with diamagnetic particles, ferromagnetic particles, super paramagnetic particles, or paramagnetic particles.

Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such as RNA or DNA, from a solution cleared of disrupted biological material using the same type or a different type of paramagnetic particle. Kits are also disclosed for use with the various methods of the present invention. Nucleic acids isolated according to the present methods and using the present kits are suitable for immediate use in downstream processing, without further purification.

The invention discloses a whole blood DNA (deoxyribonucleic acid) extraction kit based on a paramagnetic particle method and an application of the extraction kit. The kit comprises a cell lysis solution, a binding solution, a washing solution I, a washing solution II and a nucleic acid eluent. Compared with kits in the prior art, the kit has the advantages of simple and quick operation, use safety, high extraction efficiency, low manufacturing cost and the like; no toxic reagents or organic solvents are used when the kit is used, a blood sample is not required to be pre-treated, the extraction process can be finished manually and can also be automatically finished in a full-automatic nucleic acid extraction instrument, 1-16 animal tissue samples can be treated each time with a 96-hole reaction plate during automatic extraction, other operation is not required in the extraction process, time is saved for whole blood DNA extraction and detection, and the cost is reduced; DNA in the blood can be quickly extracted with the adoption of the kit, the concentration and the purity of extracted nucleic acid are high, the repeatability is good, and the kit can be used for scientific research and clinical detection.

Methods for isolating a compound from a multipart, typically biological sample. The methods use at least one paramagnetic particle having an associated electronic charge to bind compounds with the opposite charge to form a particle/compound complex. Alternatively, the paramagnetic particles have a ligand or functional group with an affinity for a target compound to form a particle/compound complex. The complex can be immobilized by applying a magnetic field to the particle/protein complex. The sample may be further processed to obtain a protein sample in a more pure form or a sample depleted of select compounds.

The invention relates to a method for extracting a free nucleic acid by using a paramagnetic particle method, which comprises the following steps: adding 300 mu l of a cracked binding solution and 20 mu l of magnetic nanoparticles into 200 mu l of a sample, mixing, then carrying out first magnetic separation on the obtained mixture to remove supernatant obtained after the first magnetic separation is implemented; adding 0.5-1 ml of washing liquid into the obtained object, mixing, carrying out second magnetic separation on the obtained mixture, and removing supernatant obtained after the second magnetic separation is implemented; adding 40-100 mu l of eluent into the obtained object, mixing, standing the obtained mixture for 5-10 min, wherein the standing temperature is 20-65 DEG C; and carrying out third magnetic separation on the obtained object, taking supernatant obtained after the third magnetic separation is implemented, so that the free nucleic acid is obtained. The invention also relates to a kit for extracting the free nucleic acid by using a paramagnetic particle method. The method for extracting the free nucleic acid by using the paramagnetic particle method disclosed by the invention has the advantages that the method is simple in operation, increases the extraction efficiency and the extraction purity, improves the sample quantity, and is applicable to the extraction of nucleic acids with short fragments.

A method for obtaining nucleic acids from a sample, includes: providing the sample including nucleic acids and other components; providing paramagnetic particles including a metal oxide core and a hydroxysilane (preferably a hydroxyalkyltrialkoxysilane) coating; contacting the sample with the paramagnetic particles under binding conditions such that the nucleic acids bind to the paramagnetic particles to provide loaded particles; separating the loaded particles from the other components of the sample; and releasing the nucleic acids from the loaded particle under eluting conditions to obtain the nucleic acids. A paramagnetic particle including a metal oxide core and a hydroxysilane (preferably a hydroxyalkyltrialkoxysilane) coating, and a kit including the particle are also described.

A microimmunoassay arrangement including paramagnetic particles having labeled antibody held in a microchannel by an external magnetic field provides rapid analysis using small sample volumes.

The invention discloses a kit for extracting nucleic acid from bacteria by using a paramagnetic particle method and an extracting method. The kit comprises a bacteria lysate, a magnetic bead binding solution, a magnetic bead scrubbing solution and a nucleic acid eluent. The bacteria lysate comprises sodium dodecyl sulfate, ethylenediaminetetraacetic acid, tri-hydroxymethyl aminomethane and sodium chloride; the magnetic bead binding solution comprises polyethylene glycol-8000 and sodium chloride; the magnetic bead scrubbing solution comprises ethanol; and the nucleic acid eluent comprises tri-hydroxymethyl aminomethane and ethylenediamine tetraacetic acid. The kit comprises the unique bacteria lysate which has a strong lysis function for the bacteria; and the kit can be used for manual extraction and instrument extraction by using a commercially available nucleic acid isolation machine, high-purity and high-concentration bacteria nucleic acid can be extracted.

The present invention relates to novel compositions of polymer-coated paramagnetic particles, defined as paramagnetic particles non-covalently coated with polymeric materials, which optionally possess targeting ligands, therapeutic agents, or carrier ligands. By selecting from a variety of linker groups and targeting ligands the coated paramagnetic particles are suitable for a wide variety of methods for controlled delivery of the particles. The invention also relates to methods and uses of imaging, diagnosing, and treating diseased or cancerous tissue using the particles.

The invention belongs to the technical field of molecular biology, and particularly relates to a reagent kit for extracting virus DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) based on a magnetic bead method and a use method of the reagent kit. The reagent kit comprises lysate, a washing liquid 1, a washing liquid 2, a washing liquid 3, an eluent and a magnetic bead suspension. A buffer solution system of the reagent kit can remove protein, pigment, greases and other inhibitory impurities in specimens to the largest extent by matching with a special nanometer magnetic beads, so that the extracted DNA or RNA has the advantages of complete fragment, high yield, high purity, stability and reliability and low cost.

The present invention relates to multi-polymer-coated magnetic nanoclusters, aqueous magnetic fluids comprising same, and methods of their use in separation procedures. The multi-polymer-coated magnetic nanoclusters comprise a super paramagnetic core, with a first polymer attached thereto, which does not render the first polymer-super paramagnetic particle complex colloidally stable, and a second polymer attached thereto, which stabilizes the complex of multi-polymer-coated magnetic nanoparticles. Methods of use comprise methods of separation, including separation of expressed protein from cells and viruses expressing the same.

The invention discloses a quantitative detection kit of a hepatitis B virus (HBV) nucleic acid applied to the field of biomedical clinic diagnosis. The kit comprises a paramagnetic particle method extraction kit and an HBV nucleic acid amplification kit, wherein the paramagnetic particle method extraction kit comprises a pyrolysis binding solution, a rinsing solution, an eluant and magnetic bead liquid; the HBV nucleic acid amplification kit comprises an HBV-PCR (Polymerase Chain Reaction) reaction solution, an enzyme mixed solution, an HBV-interior label, HBV quantitative reference products 1-4, a negative quality product, a clinical positive quality product and a strong positive quality product. The quantitative detection kit is simple, convenient and fast in operation, low in cost, high in detection sensitivity, good in repeatability, high in conservative property of primer and probe, and strong in specificity, and covers different subtypes or variants of the hepatitis B virus, improvement of the accuracy and the specificity of the hepatitis B detection is facilitated, an efficient interior label system is led in, the problems such as reciprocal inhibition, interference and the like caused by simultaneous amplification of a target gene and the interior label are solved, the overall PCR amplification process can be effectively monitored, and a false negative result is avoided.

The invention discloses a nucleic acid extraction method by virtue of a paramagnetic particle method. The nucleic acid extraction method comprises the following steps: sub-packaging 'N' [mu]M (M refers to mol/L) of sample ('N' is less than or equal to 300 and greater than or equal to 50), 'N' [mu]M of lysate and '0.1N' [mu]M of protease K in a No.1 nucleic acid extraction plate, conducting a cracking reaction at 70 DEG C for 10-60min, and then adding '1.1N' [mu]M of magnetic particle-binding buffer; sub-packaging '2.5N' [mu]M of washing solution A, '2.5N' [mu]M of washing solution B and '0.25-0.5N' [mu]M of TE solution in No.2, No.3 and No.4 nucleic acid extraction plates; and under the action of magnetic force, adsorbing magnetic particles in the No.1 nucleic acid extraction plate by virtue of a magnetic bar, conducting transferring, releasing, rapid mixing and magnetic absorption and sequentially processing the magnetic particles by virtue of the No.2, No.3 and No.4 nucleic acid extraction plates, so as to obtain extracted nucleic acid. With the application of the method disclosed by the invention, the influence of impurities on nucleic acid extraction from saliva and other biological samples is effectively reduced, so that nucleic acid extraction efficiency and nucleic acid purity are improved.

The invention belongs to the technical field of molecular biology, and particularly relates to a kit for extracting genome DNA (Deoxyribose Nucleic Acid) from plant leaves based on a paramagnetic particle method and an extracting method thereof. The kit for extracting genome DNA from plant leaves based on the paramagnetic particle method comprises a pretreatment solution, a rapid cracking liquid, a DNA binding liquid, a magnetic bead suspension and an eluent, wherein cell walls and cell membranes can be effectively cracked through the pretreatment solution; and the interference of impurities such as polysaccharides, polyphenol, tannin and the like on genome DAN extraction can be effectively eliminated through the pretreatment solution. In the rapid cracking liquid, guanidine hydrochloride and Tween 20 are taken as main components, so that plant cells can be fully cracked at the normal temperature within 2 minutes, only two minutes are required in a subsequent centrifuging step, and the operation time is greatly saved. The guanidine hydrochloride is a strong denaturant, has a good cell cracking effect. Moreover, organic solvents such as chloroform and the like are not required to be added, so that damages to operating personnel are avoided, and safety and reliability are achieved.

The invention relates to a high-precision nucleic acid quantitative detection kit for hepatitis C virus (HCV) applied to the field of biomedical clinical diagnosis. The kit comprises a nucleic acid extraction kit based on a paramagnetic particle method and an HCV nucleic acid amplification box, wherein the nucleic acid extraction kit based on the paramagnetic particle method comprises a splitting combining liquid, a rinsing liquid A, a rinsing liquid B, a rinsing liquid C, an eluting liquid and a magnetic bead liquid. The HCV nucleic acid amplification box comprises an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction liquid, enzyme mixed liquor, an HCV-interior label, an HCV quantitative reference 1-4, a negative quality control product, a critical positive quality control product and a positive quality control product. The splitting combining liquid in the nucleic acid extraction agent based on the paramagnetic particle method comprises 0.5-2.5% of lauryl sodium sulfate, 0.5-2.0ml/100ml of TritonX-100, 2-6mol/L of guanidinium isothiocyanate and 1-10mM of EDTA (Ethylene Diamine Tetraacetic Acid) (PH 7.5). The kit provided by the invention is simple and fast to operate, low in cost, high in coverage of primer and probe genotype, high in conservative property, strong in specificity, high in detection sensitivity and good in repeatability, and can better simulate the extraction state of real viruses.

A composition and method for the purification of nucleic acid are disclosed. The composition includes at least one alkaline agent and at least one detergent. The composition preferably also includes a suspension of paramagnetic particles and an acidic solution. The method involves the use of the composition with paramagnetic particles to extract nucleic acid from a biological sample.

The invention belongs to the field of molecular biology and particularly relates to a kit for extracting ribonucleic acid (RNA) by virtue of a paramagnetic particle method and an extraction method. The kit contains tissue digestion fluid, lysate, proteinase K, DNase I, DNase IBuffer, nucleic acid, extraction magnetic beads, washing liquid I, washing liquid II and eluant. The invention further discloses a method for extracting the tissue RNA by virtue of the kit. According to the kit and the method, the extraction yield and purity of RNA are increased, the integrity of RNA is improved, meanwhile, the automatic extraction is realized, and the simultaneous parallel testing of multiple samples is realized, so that the labor cost and the time cost are saved.