The invention relates to the technical field of serum zinc detection and especially relates to a serum zinc detection reagent. A reagent R1 contains a buffer, salicylaldoxime, dimethylglyoximate, a fluorocarbon surfactant FSO-300, 2, 9-dimethyl-1, 10-phenanthroline, ascorbic acid, potassium ferrocyanide, polyethylene glycol 6000, glycol, trisodium citrate, NaCl and an antiseptic. A reagent R2 contains a buffer, dimethyl sulfoxide, nitro-PAPS, a fluorocarbon surfactant FSO-300, ascorbic acid, potassium ferrocyanide, polyethylene glycol 6000, glycol, trisodium citrate, NaCl and an antiseptic. The Tris buffer, the stabilizing agent and a plurality of anti-interference agents are used, can effectively eliminate interference caused by copper, iron, calcium and magnesium ions and can substantially improve reagent stability. The used novel non-ionic fluorocarbon surfactant FSO-300 can promote serum zinc cracking, prevent reaction system turbidity, improve chromogenic substance stability, prevent a pretreatment process such as protein removal and substantially improve reagent sensitivity and stability.