11results about How to "Promote lysis" patented technology

The application relates to the technical field of nanomaterial preparation, and particularly discloses a carbon nanotube preparation method based on carbon source and plasma time sequence cooperation and a carbon nanotube. In the gas phase deposition process, carbon source gas is input in a pulse mode, and a pulse type plasma is synchronously applied, wherein the carbon source input pulse and the plasma output pulse are time-differently matched, the carbon source supply window and the plasma action window form a non-fully-synchronized time sequence coupling relationship, the carbon source activation, transportation, deposition and structure reconstruction process are controlled in the time dimension, and meanwhile, the carbon source gas is introduced from a position close to the plasma action area to maintain the pulse opening and closing characteristics. The method can improve the growth selectivity of the carbon nanotube, reduce the defect density, and is suitable for controllable preparation of single-wall carbon nanotubes. The single-wall carbon nanotube with a semiconductor content greater than or equal to 83% can be prepared with high selectivity, and the product has the advantages of few defects, high purity and high yield.

The application discloses a method for preparing biofuel by low-temperature dechlorination of waste PVC and coupling biomass co-pyrolysis, which comprises the following steps: drying, crushing and screening waste PVC, and then performing low-temperature dechlorination in an inert atmosphere, wherein the carrier gas is inert gas, the dechlorination temperature is 340-380 DEG C, the dechlorination time is 20-40 min, and dechlorinated PVC is obtained; crushing and drying the dechlorinated PVC, and then mixing the dechlorinated PVC with biomass at a mass ratio of 0.1-10:1, and then heating and pyrolyzing at 500-700 DEG C for 20-40 min to obtain biofuel. The method achieves good dechlorination effect through step-by-step pyrolysis, and improves the quality of biofuel; the method has a simple process flow, does not need to add any catalyst and adsorbent, and has a good industrial application prospect.

The present application relates to the technical field of food safety, and particularly relates to a low-power water bath ultrasonic foodborne pathogenic bacteria lysis method and application thereof. The present application uses a low-power water bath ultrasonic machine, adds ABS small balls with a size of about 1 mm in a solution sample, uses ultrasonic to cause high-frequency vibration of the ABS small balls, and the local high sound pressure field and high-density cavitation field generated by the vibration can rapidly lyse bacteria. Meanwhile, the movement of the small balls can accelerate the rapid material exchange of the solution at different positions in the tube, so that 1 mL of the to-be-tested bacterial sample can be lysed within 3 minutes. The method provided by the present application has high lysis efficiency and short time consumption. When applied to foodborne pathogenic bacteria detection, nucleic acid purification and enrichment of the lysis liquid are not needed, the time and labor cost required for nucleic acid detection can be greatly shortened, and a new method of efficient, economical and practical bacterial lysis and nucleic acid extraction is provided for the field of food safety detection.

This invention provides a method for increasing the content of benzene and naphthalene compounds in coal pyrolysis tar. The method includes: reacting low-rank coal pyrolysis volatiles sequentially through a carbon-based catalyst layer and a molecular sieve catalyst layer at a temperature of 500-800℃ under a protective gas atmosphere; the reaction products are then condensed to obtain tar. This method utilizes a series of carbon-based and molecular sieve catalysts for the catalytic cracking of low-rank coal pyrolysis volatiles, fully leveraging the abundant active sites of the carbon-based catalyst and the high selectivity of the molecular sieve. Through the synergistic effect of the carbon-based and molecular sieve catalysts, a significant increase in the content of benzene and naphthalene compounds in the tar obtained from coal pyrolysis is achieved.

The application provides a kind of preparation of red radish protoplast enzymatic hydrolysate and the preparation method and application of red radish protoplast, belong to the technical field of plant cytology.The enzymatic hydrolysate includes:1.0~1.5w / v% cellulase, 0.3~0.5w / v% dissociation enzyme and 0.3~0.5w / v% hemicellulase.The cellulase has the effect of degrading cellulose in plant cell wall, dissociation enzyme is used with cellulase, which is beneficial to separate plant tissue into single cell, and the main effect of hemicellulase is to degrade hemicellulose in plant cell wall.The enzymatic hydrolysate can synergistically promote the effect of red radish tissue cell wall lysis through the comprehensive effect of three enzymes, can better and faster destroy cell wall skeleton, is more conducive to the preparation of red radish protoplast and further maximally release protoplast, is conducive to improving the yield of protoplast, and can also keep the protoplast with high activity.

The application belongs to the technical field of polylactic acid production, and particularly relates to a polylactic acid intermediate reaction-separation integrated device and a reaction-separation process. The polylactic acid intermediate reaction-separation integrated device comprises a reaction tower, the reaction tower comprises a packing tower body and a plate tower body integrated together, the reaction tower is provided with a lactic acid oligomer feeding port, a tower top light component discharge port, a tower bottom heavy component discharge port and a lactide intermediate discharge port, the tower bottom heavy component discharge port is used for being communicated with a lactic acid oligomer storage system, the reaction tower is further provided with a heat exchanger, and the tower top light component discharge port is connected with a tower top condenser; the plate tower body is provided with a feeding distributor and at least two layers of staggered tower plates arranged in an upper and lower interval, and each layer of tower plate is provided with a heating pipe. The lactic acid oligomer cracking and the lactide purification are integrated in one reaction tower, two processes are combined into one process, the process flow is shortened, and the further polymerization of lactic acid oligomer and the generation of side reactions are reduced.

The utility model belongs to the technical field of biomass gasification, and discloses a novel clean fuel gas biomass fixed bed gasification furnace which comprises an outer reaction furnace and an inner reaction furnace, the inner reaction furnace is coaxially arranged in the outer reaction furnace, the bottom of the inner reaction furnace and the inner bottom of the outer reaction furnace are arranged at intervals, and a fuel gas generation area is arranged in the inner reaction furnace. The top of the inner reaction furnace extends to the outside of the outer reaction furnace, a furnace bar is fixed in the inner reaction furnace, the outer peripheral wall of the inner reaction furnace and the inner peripheral wall of the outer reaction furnace are spaced to form a gas purification area, and a gas outlet is formed in the side wall of the top end of the outer reaction furnace; a feeding mechanism is arranged above the inner reaction furnace; a bottom opening of the gas purification area is provided with a filtering mechanism; a cooling mechanism is fixed on the outer side wall, corresponding to the fuel gas purification area, of the inner reaction furnace; an ash removal mechanism is mounted at the bottom in the outer reaction furnace, and an ash inlet end of the ash removal mechanism axially corresponds to an opening in the bottom end of the inner reaction furnace. According to the reaction furnace, the temperature is kept stable by absorbing reaction heat, the gasification efficiency is improved, and the purification effect of fuel gas is improved.

This invention discloses a green and efficient pretreatment method for biomass and its application in the selective preparation of 2,3-dihydrobenzofuran. The pretreatment includes: uniformly mixing biomass raw materials with a ternary eutectic solvent, reacting the mixture in a microwave synthesizer, and then washing, filtering, and drying the reactants to collect the solid residue, thus obtaining the pretreated biomass. The ternary eutectic solvent used in this invention is a mixed solution of zinc chloride, ethylene glycol, and water. It is simple to prepare, uses readily available and harmless raw materials, and is recyclable. Zinc has the dual functions of solvent and catalyst, increasing the total number of hydrogen bonds, promoting the breaking of β-O-4 bonds, and increasing the acidity of the solvent. It also forms metal ion complexes with water ligands, promoting biomass pyrolysis. The pretreatment method of this invention can achieve a lignin removal rate of over 80% at low temperatures, requires a short reaction time, uses readily available and harmless solvents, and exhibits a selectivity for 2,3-dihydrobenzofuran exceeding 40% after pyrolysis, providing technical support for advancing industrialization.

The present application relates to the technical field of biomass energy conversion, and particularly relates to a device for preparing bio-crude oil through continuous hydrothermal liquefaction of biomass, which comprises a pretreatment unit, a hydrothermal reactor, a gas-solid-liquid separator and an oil-water separator connected through pipelines in sequence, and further comprises a catalyst circulation unit for converting solid residues into catalysts, the catalyst circulation unit comprising an activation reactor and a conditioning tank connected in series, the activation reactor being connected in parallel to a feed pipeline of the hydrothermal reactor, and the conditioning tank being connected in parallel to a solid outlet of the gas-solid-liquid separator. According to the present application, the solid residues are converted into catalysts, so that the catalysts are high-performing, the catalysts are recycled during hydrothermal liquefaction, and the bio-crude oil is further upgraded.

This application relates to the field of catalysts for in-situ oil shale extraction, and particularly to a catalyst for simultaneous hydrogen production during in-situ oil shale extraction and its preparation method. The preparation method includes the following steps: S1, preparation of a pretreated support: S101, preparation of an intercalated support; S102, oleophilic modification; S2, metal loading: S201, immersing the pretreated support obtained in S1 into a metal impregnation solution, stirring, and then concentrating under reduced pressure to obtain a metal-loaded support; S202, adding molybdenum disulfide and nickel phosphide to the metal-loaded support, adding a dispersant, and stirring to obtain a doped support; S203, aging the doped support in a sealed environment to obtain an aged support; S3, catalyst molding. This application provides a catalyst for simultaneous hydrogen production during in-situ oil shale extraction and its preparation method to solve the problems of low energy utilization efficiency and the inability to simultaneously achieve shale oil extraction and hydrogen production in related technologies.

This invention relates to the field of molecular detection technology, and in particular to viral nucleic acid sample diluents, viral nucleic acid sample extraction kits, and viral nucleic acid extraction methods. The viral nucleic acid sample release agent provided by this invention is compatible with various sampling kits. By adjusting the types and proportions of components, the critical micelle concentration of the surfactants used is minimized, ensuring lysis of cells and pathogens while reducing inhibitory effects on subsequent detection systems. With the addition of ion exchange resin, this invention can adsorb proteins and metal ions such as calcium and magnesium in the sample that affect subsequent reactions, promoting cell and viral lysis and enhancing system compatibility. The detection system of this invention has good compatibility, simultaneously compatible with multiple qPCR detection systems and also usable in isothermal RAA amplification systems. It is suitable not only for liquid detection systems but also for dry powder detection systems with large sample volumes.