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Anti-bacterial peptide, anti-bacterial peptide hydrogel and preparation method of anti-bacterial peptide hydrogel

An antimicrobial peptide and hydrogel technology, applied in the field of biopharmaceuticals, can solve the problem that mechanical debridement cannot completely remove biofilm, and achieve the effect of promoting rapid healing and promoting growth and proliferation

Active Publication Date: 2019-03-12
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above methods have their own disadvantages. Among them, mechanical debridement cannot completely remove the biofilm, and any residue will quickly restore the biofilm; due to the multi-drug resistance mechanism of the biofilm, it is difficult for antibiotics to completely kill the bacterial biofilm. ; Nano-silver preparations and existing antimicrobial peptides (such as defensins) will also produce non-negligible toxicity to normal tissues while killing bacteria

Method used

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  • Anti-bacterial peptide, anti-bacterial peptide hydrogel and preparation method of anti-bacterial peptide hydrogel
  • Anti-bacterial peptide, anti-bacterial peptide hydrogel and preparation method of anti-bacterial peptide hydrogel
  • Anti-bacterial peptide, anti-bacterial peptide hydrogel and preparation method of anti-bacterial peptide hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 solid-phase synthesis method prepares antimicrobial peptide

[0035] First, the carboxyl group of Fmoc-asparagine is connected with an insoluble polymer resin (as a solid phase carrier) in a covalent bond structure, and then the amino group of asparagine and the carboxyl group of Fmoc-valine are condensed through deprotection Form a peptide bond, repeat the above peptide bond formation reaction, and gradually connect serine, leucine, tyrosine, lysine and isoleucine to the resin, so that the peptide chain grows from the carboxyl terminal to the amino terminal until it reaches The desired length of the peptide chain is finally cut off from the resin with hydrofluoric acid to obtain antimicrobial peptide 1. In a specific embodiment, the insoluble polymer resin is king resin.

[0036] First, the carboxyl group of Fmoc-asparagine is connected with an insoluble polymer resin as a solid phase carrier in a covalent bond structure, and then the amino group of aspar...

Embodiment 2

[0039] Example 2 Antibacterial Activity and Cytotoxicity Detection

[0040] This embodiment detects the antibacterial activity and cytotoxicity of the antimicrobial peptide 1 and antimicrobial peptide 2 prepared in Example 1. The antibacterial activity is taken as an example of Escherichia coli, and the results are shown in Figure 1 ~ Figure 2 .

[0041] The results showed that antimicrobial peptide 1 and antimicrobial peptide 2 had similar antibacterial ability (Escherichia coli) compared with the control group (no polypeptide addition) and the comparative example 1 group (existing antimicrobial peptide defensin). Compared with the control group (no peptide addition), antimicrobial peptide 1 and antimicrobial peptide 2 have basically no effect on the growth of normal cells, showing excellent biocompatibility, while comparative example 1 group (existing antimicrobial peptide defensin) has no effect on Normal cells have certain toxicity and poor biocompatibility.

Embodiment 3

[0042] Example 3 Preparation of Antimicrobial Peptide 1 Hydrogel and Detection of Drug Release Efficiency

[0043] Weigh 4 mg of purified lyophilized antimicrobial peptide 1 powder into a 1.5 mL sample bottle, and then add 400 μL of a solution in which photothermal reagent (20 μg indocyanine green ICG) and wound growth-promoting factor (5 mg of proline) are dissolved , so that the final concentration of antimicrobial peptide 1 was 10 mg / mL, sonicated until the powder of antimicrobial peptide 1 was fully dissolved, and left to stand for 10 minutes to make it self-assemble into a gel. The results of atomic force electron microscopy and scanning electron microscopy are shown in image 3 .

[0044] This example further investigated the release efficiency of the photothermal reagent (ICG) and wound growth-promoting factor (proline) loaded in the antimicrobial peptide 1 hydrogel at different pHs. The results are as follows Figure 4~5 shown.

[0045] Depend on Figure 3-5 It can ...

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Abstract

The invention relates to the technical field of biological pharmacy, in particular to an anti-bacterial peptide, an anti-bacterial peptide hydrogel and a preparation method of the anti-bacterial peptide hydrogel. The amino acid sequence of the anti-bacterial peptide is shown as SEQ ID NO:1. Experiments show that the polypeptide of the anti-bacterial peptide has high antibacterial activity and excellent biocompatibility. The anti-bacterial peptide hydrogel is made of the anti-bacterial peptide, a photo-thermal reagent and a wound growth promoting factor. Tests show that the anti-bacterial peptide hydrogel has a good killing effect on bacteria, especially drug-resistant bacteria, on the surface of a biological film, a bacterial biofilm can be effectively spitted and removed, growth and proliferation of wound epidermal cells, collagen formation and angiogenesis are facilitated, and rapid healing of wounds infected by the bacterial biofilm is achieved.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to an antimicrobial peptide, an antimicrobial peptide hydrogel and a preparation method thereof. Background technique [0002] After human skin is injured, normal tissue healing will go through processes such as hemostasis, inflammation, proliferation and tissue remodeling. The series of wound healing processes are not only complicated, but also fragile, and are easily disturbed by various factors and stagnated, thus forming chronic wounds. Chronic wound infection constitutes 60-80% of human infectious diseases. In addition, with the aging of the social population and the increasing prevalence of diseases such as diabetes and obesity, chronic wounds have become one of the main problems of global health. Bacterial biofilm infection is relatively common in chronic wounds. Bacterial biofilm refers to the membrane-like bacterial colony formed by bacteria adhering to the sur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/06C07K1/04A61K9/06A61K38/08A61K41/00A61K45/06A61P31/04A61P17/02
CPCA61K9/06A61K38/08A61K41/0052A61K45/06A61P17/02A61P31/04C07K7/06A61K2300/00
Inventor 李永强王建浩赵远杜炫呈吴春
Owner SUZHOU UNIV
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