78 results about "Peptide bond" patented technology

This invention provides a tetrahedral antibody comprising a first, second, third, and fourth domain, wherein the first and second domains are Fab or Fc domains; wherein each of the first and second domains comprise a first polypeptide chain comprising a first N-terminus of the domain, and a second polypeptide chain comprising a second N-terminus of the domain; wherein the first N-terminus of the first domain and the first N-terminus of the second domain are joined to each other by a non-peptidyl linkage, which can be a covalent linkage or a non-covalent linkage between first and second dimerizing polypeptides attached to the first N-termini of the first and second domains, respectively; and wherein the third and fourth domains are attached at their respective C-termini to the second N-termini of the first and second domains, respectively, or the N-termini of the first and second dimerizing polypeptides.

The invention relates to molecular glue for forming fimbriae related protein based on SpaA isopeptide bonds of Vinci bacteria. The invention also relates to a nanoparticle vaccine composition formed by the molecular glue, and the immunogen is covalently displayed on the surface of the nanoparticle by the molecular glue.

This invention discloses methanogenic lyases with the same protein family annotation as PeiR lyases and their applications. These enzymes are obtained through homology screening based on the known characteristics of PeiR lyases targeting peptide bonds in the methanogenic cell wall, using the Pfam protein family database annotation. PeiR lyase proteins participate in the biological process of hydrolyzing archaea cell walls, effectively killing methanogens and reducing methane production. Proteins homologous to this protein have potential methane-reducing potential. This invention integrates rumen microbial metagenomic sequencing data, uses a series of bioinformatics software to screen methanogenic viral proteins, and combines Pfam functional domain annotation for homology analysis, ultimately identifying a series of lyases. These lyases were successfully expressed in a prokaryotic expression system, and in vitro gas production experiments also showed that the crude enzyme solution significantly reduced methane production.

This invention belongs to the field of photocrosslinking probe technology, specifically relating to a GUSB photocrosslinking probe, its preparation method, and its application. The GUSB photocrosslinking probe has the general chemical formula C0. 13 H 18 N2O6-R, wherein R is any one of the oxalic group elements or peptide bonds. The R group covalently connects the glucuronic acid recognition core and the photosensitive unit in the GUSB photocrosslinking probe, and the R group contains no more than 4 atoms, resulting in a small size and low steric hindrance. The GUSB photocrosslinking probe provided by this invention uses glucuronic acid as the recognition core, combined with a small-volume diazacyclopropane photosensitive unit and a terminal alkyne group, to achieve µM-level in-situ covalent capture of active GUSB in complex systems such as cells or tissues. The probe of this invention significantly improves positioning accuracy, sensitivity, data availability, quantification, and enrichment, while also possessing advantages such as modular synthesis, mild conditions, and strong system compatibility, effectively addressing the technical shortcomings of existing technologies that struggle to accurately obtain GUSB activity in situ under low-dose conditions.

This invention discloses a methanogenic lyase homologous to PeiR lyase and its applications. The PeiR lyase protein, targeting the peptide bonds of the methanogenic cell wall, participates in the biological process of hydrolyzing the archaea cell wall, effectively killing methanogens and reducing methane production. Proteins homologous to this lyase possess potential methane-reducing potential. This invention utilizes a series of bioinformatics software to identify protein sequences encoded by methanogenic viruses from a large amount of rumen microbial virome sequencing data, and analyzes the structural homology between these proteins and PeiR. Ultimately, this invention successfully obtained a series of methanogenic lyases homologous to PeiR. To achieve the expression of these lyases, this invention designed multiple primers to synthesize the target sequences and successfully expressed them in a prokaryotic expression system. In vitro gas production experiments with the crude enzyme solution showed that these lyases are effective in reducing methane production.

The present invention relates to a method for modifying meat, including bringing the meat in contact with a Bacillus amyloliquefaciens-derived neutral protease having the following properties (a) to (e), and thermolysin; (a) being an enzyme that hydrolyzes a substrate having a peptide bond to release the peptide, (b) having an optimal pH of 7, (c) being stable at pH=5 to 7, (d) having an optimal temperature of 50°C to 55°C, (e) being a metal protease inhibited by ethylenediaminetetraacetic acid (EDTA), and a composition for modification, containing said neutral protease and thermolysin. According to the present invention, meat can be tenderized conveniently in a short time, and ease of loosening can be imparted to the meat.

This invention provides a method for producing Clostridium neurotoxins containing an exogenous activation loop, modified Clostridium neurotoxins, and pharmaceutical compositions. [Solution] A method for proteolytically processing a single-chain Clostridium neurotoxin into a corresponding double-chain Clostridium neurotoxin, comprising: providing a single-chain Clostridium neurotoxin; and contacting the single-chain Clostridium neurotoxin with enterokinase or factor Xa, wherein the single-chain Clostridium neurotoxin has the polypeptide sequence Cys-(Xaa) a -Ile-Asp / Glu-Gly-Arg-(Yaa) b - A method in which an activation loop containing Cys (SEQ ID NO: 1) is present, a=1 to 10, b=4 to 15, and enterokinase or factor Xa hydrolyzes the peptide bond of the activation loop, thereby producing a double-stranded Clostridium neurotoxin.

The present invention relates to a hyaluronic acid-based hydrogel which is a hyaluronic acid-peptide crosslinked body crosslinked using a peptide crosslinking agent. More specifically, the present invention relates to a hyaluronic acid-based hydrogel and a method for producing same, wherein a crosslinked body having novel physical properties is obtained using a relatively small amount of a crosslinking agent that forms peptide bonds, unlike conventional crosslinking agents, and the hyaluronic acid-based hydrogel has the advantages of: being safe and having few side effects; the physical properties of a filler being adjustable according to the amount of peptide crosslinking; and having a high elasticity ratio.

The invention discloses a SpaD-based polypeptide-protein reaction pair, and belongs to the technical field of protein engineering. The D2-D3 structural domain of the corynebacterium diphtheriae pilus protein SpaD is split into reaction pairs in two modes, the two parts can be efficiently recombined and react to generate isopeptide bonds, and covalent coupling is achieved. Wherein when the D2 structural domain is split into reaction pairs, a D214-T455 residue of an original sequence is a capturer protein (D2C), and an E182-K213 residue of the original sequence is a tag polypeptide (D2T); when the D3 structural domain is split into reaction pairs, the E182-D434 residue of the original sequence is a capturer protein (D3C), and the G438-T455 residue of the original sequence is a tag polypeptide (D3T). The reactivity of a reaction pair obtained by splitting D3 is equivalent to that of a common spy reaction pair, is not influenced by the position of a tag sequence, is orthogonal to the spy reaction pair and a probe reaction pair, is a novel protein coupling tool, and has a relatively high practical value in protein engineering.

The present disclosure provides a heterodimeric antibody, or antigen-binding fragment thereof, comprising two heavy chains with different amino acid sequences and a binding polypeptide. The heterodimer antibody, or the antigen-binding fragment thereof, include a first heavy chain comprising none or one or more modifications and the second heavy chain comprises one or more modifications and is fused to a binding polypeptide, which can form a isopeptide bond with a binding peptide. The present disclosure further provides systems and methods for purifying such heterodimeric antibodies, and antigen-binding fragments thereof.

This invention discloses a method for rapidly constructing a complex of cholera toxin B subunit (CTB) and antigen. The vaccine complex includes a CTB-SpyTag fusion protein and at least one SpyCatcher-antigen fusion protein. It also includes a preparation method for rapidly preparing a CTB5-antigen complex by mixing CTB-SpyTag with the SpyCatcher-antigen. This invention directs the antigen to the side of the CTB pentamer that does not participate in GM1 binding, avoiding steric hindrance interference. It utilizes the SpyTag / SpyCatcher covalent isopeptide bond to obtain highly stable antigen display, reducing antigen shedding during subsequent preparation and storage. It also possesses mucosal targeting and multivalent presentation functions, providing a universal platform technology for rapidly constructing and validating broad-spectrum, combined, or personalized mucosal vaccines.

The application provides a recombinant polymyxin enzyme and a preparation and application method thereof, an amino acid sequence of the recombinant polymyxin enzyme is shown as SEQ ID NO. 1, and the recombinant polymyxin enzyme is obtained through site-directed mutation on an amino acid sequence of a wild-type polymyxin enzyme derived from Brevibacillus laterosporus. The recombinant polymyxin enzyme can specifically recognize and cut a peptide bond between a tripeptide side chain and a cyclic heptapeptide ring in a polymyxin structure and a peptide bond between threonine and diaminobutyric acid inside the cyclic heptapeptide ring, and further can specifically degrade or neutralize the recombinant polymyxin enzyme of polymyxin.

This invention belongs to the food field, specifically relating to a hypoallergenic, gluten-free, non-wheat tiger nut fermented noodle product and its preparation method. The hypoallergenic, gluten-free, non-wheat tiger nut fermented noodle product comprises the following components: raw material powder, alkaline protease, transglutaminase, additives, and water; the raw material powder is a mixture of tiger nut meal powder and rice flour. The technical solution of this application utilizes a fermentation system using alkaline protease and transglutaminase. Alkaline protease hydrolyzes the amide and peptide bonds of proteins, causing acylation reactions that cross-link protein molecules, producing a network structure and improved flavor and quality. Transglutaminase catalyzes the acylation reaction between the amide group in glutamine and the ε-amino group on lysine peptides, causing cross-linking of protein molecules to form the target protein and produce the desired product.

This invention provides a recombinant Staphylococcus aureus enterotoxin B nanoparticle protein vaccine, mSEB-mi3, its preparation method, and its applications. This invention utilizes a SpyTag / SpyCather protein linker system to connect the recombinant protein to the nanoparticle protein, allowing mSEB to be displayed on the surface of self-contained nanoparticles, thus obtaining the recombinant Staphylococcus aureus enterotoxin B nanoparticle protein mSEB-mi3. Both mSEB-SpyTag and SpyCather-mi3 proteins in this invention are induced to be expressed in soluble form in Escherichia coli. The mSEB-SpyTag and SpyCather-mi3 of this invention can be covalently bound via isopeptide bonds to form the mSEB-mi3 nanoparticle protein vaccine. The binding conditions are simple, and when combined with the adjuvant MF59, it can induce rapid antibody production in mice, generating a high-level immune response and exerting a protective effect. Furthermore, its antibody response rate and protective efficiency are superior to those of the monomeric protein mSEB.

The present application aims to provide a novel peptide which shows a rapid effect of drug efficacy and potency and has a long relief maintenance period, a medicament and an external agent containing the same, and particularly a preventive or therapeutic agent for dermatitis, rough skin, rhinitis, alopecia, thin hair, a hair growth agent, a hair growth promoting agent, an antipruritic agent, a skin care product, and the like. The present application achieves the above aim by providing an amino acid sequence represented by Formula I, a cyclic peptide or a derivative thereof having no peptide bond other than between the amino acids constituting the amino acid sequence, or a pharmaceutically acceptable salt thereof.

The present application relates to a kind of tumor polypeptide vaccine. Specifically, the present application provides a kind of adjuvant-antigen peptide bond, and the adjuvant-antigen peptide bond is the structure shown in formula (I). The adjuvant-antigen peptide bond of the present application has excellent solubility, good drug property, and has excellent immune activation ability, can more effectively activate T cell, thereby play excellent immunotherapy effect, and has excellent treatment effect on tumor.

The invention discloses a synthesis method of a protein ligase mediated specific modified polypeptide, and belongs to the technical field of bioengineering. The problem that in the prior art, phosphorylated Tau protein is difficult to produce on a large scale is solved, Tau polypeptide is used for replacing full-length site specific phosphorylated Tau protein to serve as a positive standard substance in a clinical diagnosis kit, protein ligase is utilized for catalysis, segmented synthesis of polypeptide containing phosphorylated specific modification is achieved, and the specific phosphorylated Tau protein is obtained. Polypeptides from different sources can be efficiently and directionally connected under mild conditions, so that target proteins or polypeptide molecules with complete structures and various functions are obtained, and the peptide bond synthesis production efficiency is effectively improved.

A polymer having a backbone chain, wherein the backbone chain includes linear amide linkages and nitrogen-atom-containing heteromonocyclic moieties, wherein the backbone chain is free of peptide linkages or wherein the backbone chain has a proportion of peptide linkages of maximum 30%, based on a total number of the linear amide linkages of the backbone chain, and wherein the nitrogen-atom-containing heteromonocyclic moieties have a proportion of 5% by weight to 25% by weight, based on the total weight of the polymer, is provided. A product or article and the use of the polymer for manufacturing or producing the product or article are further provided.

This invention provides a recombinant Staphylococcus aureus nanoparticle protein HI-mi3, its preparation method, and its applications. This protein is composed of HI (containing the key SA virulence factor α-hemolysin Hla). H35L The fusion protein HI and mi3 are composed of the N2 functional domain of the iron ion surface determinant B and self-assembled nanoparticle protein mi3. This invention uses the SpyTag / SpyCatCher protein coupling system to link the fusion protein antigen HI and mi3, obtaining the Staphylococcus aureus self-assembled nanoparticle protein HI-mi3. Both the recombinant protein SpyCatcher-mi3 and the recombinant protein SpyTag-HI of this invention are induced to be expressed in soluble form in Escherichia coli. The recombinant protein SpyCatcher-mi3 of this invention can covalently bind to the recombinant protein SpyTag-HI via isopeptide bonds to form the HI-mi3 nanoparticle vaccine molecule, with simple binding conditions. The HI-mi3 nanoparticle vaccine of this invention can induce a high level of immune response in animals and exert an immunoprotective effect.

The invention provides an engineered probiotic-polymer conjugate and application thereof, SpyCatcher protein is displayed on the surface of probiotics, a polymer comprises a SpyTag residue and a dopamine structure part, and the probiotics and the polymer are coupled through an isopeptide bond formed between the SpyCatcher and the SpyTag. The colonization ability of the conjugate in the gastrointestinal tract is remarkably improved, the tolerance of the probiotics in the gastrointestinal tract is effectively improved, the construction method of the conjugate does not affect the inherent probiotic activity of the probiotics, and the conjugate has important significance in treatment of gastrointestinal diseases and is expected to promote the development of engineered intestinal microorganisms.

The application discloses a preparation method and application of a fluorescent peptide bond structure water treatment agent, and a chemical structure thereof is shown in the following formula: wherein A represents an unmodified structure unit, a polymerization degree of which is n; B represents a modified structure unit, a polymerization degree of which is m; n+m represents a total polymerization degree; and m / (n+m) * 100% = 40% to 70%. The polyaspartic acid derivative prepared by the application can not only effectively chelate and coordinate with metal ions in circulating water, but also can form a protective film on the surface of carbon steel, so that the corrosion of carbon steel and other metals by atmosphere and harmful substances is reduced, and the efficient promotion of the scale inhibition performance and corrosion inhibition performance of polyaspartic acid is realized. In addition, the water treatment agent can realize online detection of different dosages and concentrations according to the fluorescence intensity, so that the best performance of the agent is exerted, the concentration multiple of the circulating water is improved, water resources are saved, and the purpose of energy saving and emission reduction is achieved.

The invention provides zero-additive soy peptone powder and a preparation method thereof, and relates to the field of food processing. Comprising the following steps: performing magnetic separation and impurity removal treatment on raw materials; adjusting the water content of the raw materials to 50-60%; adding the raw materials into instantaneous ejection steam explosion equipment, then treating for 5-15 minutes under 0.8-2.5 MPa saturated steam, completing pressure relief within 0.00875 seconds, and triggering ejection explosion of the raw materials; collecting a product, carrying out centrifugal separation treatment, realizing solid-liquid separation and grease separation to obtain pure small peptide liquid, and carrying out electrodialysis sodium removal treatment on the pure small peptide liquid to obtain low-sodium small peptide liquid; and carrying out nanofiltration membrane concentration and grading treatment, and carrying out spray drying to obtain the additive-free soy peptone powder. Through the synergistic effect of millisecond-level pressure relief ejection of high-pressure saturated steam (0.8-2.5 MPa), physical-level structure dissociation and peptide bond directional breakage of the protein raw material are realized.

The purpose of the present invention is to provide a component capable of enhancing saltiness. Provided is an oligopeptide that is a degradation product of γ-glutamyl bonds and peptide bonds of a pea protein and has a molecular weight of 900-1000 Da, specifically an oligopeptide that is obtained by causing glutaminases and proteases to act on a pea protein, that has a molecular weight of 900-1000 Da, and that can enhance saltiness.

The invention relates to a peptide for use in prophylaxis, prevention, inhibition and / or treatment of psoriasis of a mammal, wherein the peptide comprises amino acids joined by peptide bonds, and wherein the peptide comprises an amino acid sequence of from 3 to 5 amino acids comprising 2 acidic amino acids and proline (P) (Pro). The invention also relates to a pharmaceutical composition for use in prophylaxis, prevention, inhibition and / or treatment of psoriasis of a mammal, wherein the pharmaceutical composition comprises a peptide, wherein the peptide comprises amino acids joined by peptide bonds, and wherein the peptide comprises an amino acid sequence of from 3 to 5 amino acids comprising 2 acidic amino acids and proline (P) (Pro). The invention further relates to a composition for skin improvement, a cosmetic composition, a topical skin cream composition and a nutritional compositions, wherein said compositions comprise a peptide comprising amino acids joined by peptide bonds, wherein the peptide comprises an amino acid sequence of from 3 to 5 amino acids comprising 2 acidic amino acids and proline (P) (Pro).

The invention provides a nanoparticle protein rePO-TTFC (at) LS as well as a preparation method and application thereof. The protein is composed of rePO (at) LS and a tetanus toxin C fragment TTFC. The recombinant protein SpyCatcher-rePO at LS disclosed by the invention is subjected to induced expression in escherichia coli in a soluble form. According to the present invention, the recombinant protein SpyCatcher-rePO (at) LS can be subjected to covalent binding with the recombinant protein reTTFC-SpyTag through the isopeptide bond so as to form the rePO-TTFC (at) LS duplex nanoparticle vaccine molecule, and the binding condition is simple; the nanoparticle protein can induce an animal body to generate high-level immune response and play an immune protection role, the response speed and protection efficiency of an antibody induced by the nanoparticle protein are obviously superior to those of a monomer rePO and a monomer reTTFC fusion protein, and the nanoparticle protein can be used for preventing and treating pseudomonas aeruginosa and tetanus at the same time.

The application discloses an algal protein peptide with anti-radiation activity and a preparation method and application thereof. The algal protein peptide is prepared by the following steps: adding pectinase and cellulase into red alga raw materials to release the algal protein by sol and lysis; adopting enzyme conversion to cut subunits to identify disulfide bonds and peptide bonds, so that a specific functional fragment component of the algal protein peptide is obtained; and through activity verification, the algal protein peptide with anti-radiation function is prepared in a targeted manner. The prepared algal protein peptide has a very strong protective effect on ultraviolet radiation HaCat cells, can effectively relieve cell DNA damage caused by UVB, improve cell survival rate under UVB irradiation, and reduce mortality. Therefore, the algal protein peptide can be applied to the preparation of food, cosmetics, drinks and other products, and has a good anti-radiation effect.

The invention belongs to the field of protein hydrogel materials, and particularly relates to elastin-like and a preparation method and application of elastin-like hydrogel. According to the present invention, the two elastin-like proteins A and B having different amino acid sequences are utilized to form the cross-linked network through the isopeptide bond, such that the mechanical strength of the polymer hydrogel composed of the four-arm polyethylene glycol amine, 4-arm PEG-NH2 and the o-phthalaldehyde modified four-arm polyethylene glycol, 4-arm PEG-OPA can be effectively improved, the adhesion can be improved, and the cross-linked network can be used for the preparation of the polymer hydrogel, such that the cross-linked network can be formed through the cross-linked network, such that the cross-linked network can be used for the preparation of the polymer hydrogel, and the use amount of the polymer hydrogel can be reduced. The obtained elastin-like hydrogel has the advantages of strong wet tissue adhesion capacity, good biocompatibility, injectable use and the like, and is beneficial to application in the biomedical fields of hemostasis, tissue repair and the like.

The application relates to the technical field of medical treatment and provides a CAR-gammadelta T cell targeting AXL and application thereof, the CAR-gammadelta T cell has a CAR molecule, the CAR molecule comprises the following structure: signal peptide-antiAXL scFv-extracellular hinge region-transmembrane region-co-stimulating factor-CD3 zeta intracellular region, the "-" is independently a connecting peptide or a peptide bond; the co-stimulating factor comprises a CD28 intracellular region and a 41BB intracellular region. Advantages: the CAR-gammadelta T cell targeting AXL integrates a third-generation CAR structure (CD28+4-1BB double co-stimulating domain) and a gammadelta T cell, realizes high targeting precision and killing efficiency. The in-vitro killing rate of the CAR-gammadelta T cell targeting AXL to AXL positive lung cancer cells (A549 and HCC827-ER3) is >90% (effector to target ratio 10:1), is obviously improved compared with common gammadelta T cells, and the tumor volume is obviously reduced in a PDX mouse model, which proves excellent treatment effect.

The present invention provides a compound having the structure: wherein A is a biologically active structure of the compound; wherein Z is a protein component of the compound, which protein component comprises one or more polypeptides, wherein at least one of the one or more polypeptides comprises consecutive amino acids which (i) are identical to a stretch of consecutive amino acids present in a chain of an Fc domain of an antibody; (ii) bind to an Fc receptor; and (iii) have at their N-terminus a sequence selected from the group consisting of a cysteine or selenocysteine; wherein the dashed line between B and Z represents a peptidyl linkage; and wherein the solid line between A and B represents a nonpeptidyl linkage, as well as intermediates dimers thereof, and processes of producing the compounds of the invention.

This invention relates to a genetically engineered bacterial outer membrane vesicle and its preparation method. By using in-cell peptides to attach exogenous proteins to the surface of OMVs via peptide bonds, a variety of desired antigens can be conveniently delivered. The reaction conditions are mild, less affected by antigen size, and unlike the SpyCatcher / SpyTag system, the in-cell peptides do not remain in the mature protein after attaching the exogenous protein to the OMV surface. This invention can serve as a universal tool for simultaneously or separately displaying different antigens on the surface of OMVs.