557 results about "Peptide bond" patented technology

A method for preparing a drug complex in which a polysaccharide derivative having carboxyl groups and a residue of a drug compound are bound to each other by means of a spacer comprising an amino acid or a spacer comprising peptide-bonded 2 to 8 amino acids, or a drug complex in which a polysaccharide derivative having carboxyl groups and a residue of a drug compound are bound to each other without the spacer, characterized in that an organic amine salt of the polysaccharide derivative having carboxyl groups is reacted with the drug compound or the spacer bound to the drug compound in a non-aqueous system. The reaction between the polysaccharide derivative having carboxyl groups and the drug compound bound with the spacer or the like can be carried out in high yields, and when a drug compound having a lactone ring is subjected to the reaction, side reactions can be reduced.

The invention relates to single-chain multimeric polypeptides comprising at least two units of a monomeric polypeptide linked via a peptide bond or a peptide linker, wherein the monomeric polypeptide is of a type that is biologically active in monomeric form, and to polypeptide conjugates having at least one non-polypeptide moiety covalently bound to an attachment group of the polypeptide. The polypeptide is preferably a G-CSF dimer bound to a polymer molecule, preferably to one or more polyethylene glycol molecules.

The invention discloses a biomacromolecule interpenetrating polymer network hydrogel and a preparation method of the biomacromolecule interpenetrating polymer network hydrogel. The biomacromolecule interpenetrating polymer network hydrogel is formed by crosslinking two kinds of enzymes in a catalysis mode, one is a polysaccharide macromolecule network formed by crosslinking polysaccharide macromolecules with introduced phenolic hydroxyl groups in an oxidation mode as oxidase and hydrogen peroxide catalyze phenolic hydroxyl groups, and the other is a protein or polypeptide macromolecule network formed crosslinking protein or polypeptide containing amino acid residues as transferase catalyzes peptide bonds. The two networks interpenetrate each other and form the novel interpenetrating polymer network hydrogel without bonding of chemical bonds. No chemical crosslinking agent is used in the hydrogel, and the hydrogel has excellent biocompatibility and mechanical property, can be shaped like a dry or wet film, like porous sponge or fibers, and can serve as a contact lens, a medicine release carrier, a scaffold for tissue engineering or materials for tissue repair.

A DDS compound comprising a carboxy(C_1-4)alkyldextran polyalcohol modified with a saccharide compound and a residue of drug compound bound to the carboxy(C_1-4)alkyldextran polyalcohol, and a method for measuring a DDS compound in which a polymer carrier and a residue of drug compound are bound to each other by means of a spacer comprising 2 to 8 amino acids linked by peptide bond(s), which comprises the steps of treating the DDS compound with a peptidase, and measuring the resulting hydrolysate.

Disclosed are peptide agents and uses thereof that are analogs of biologically active peptides such as somatostatin and bombesin. The compounds of the invention have the general formula X-Y-Z-Q, where X is a cytotoxic agent, therapeutic agent, detectable label or chelating group, and Q is a biologically active peptide. In peptide agents of the invention Y is optionally a hydrophilic polymer or peptide, and Z is a linking peptide bonded to Q at the amino terminus of Q, having two, three, four, or five, amino acid residues selected to link X to Q, while retaining the biological activity of Q. Methods of using these peptide agents in the diagnosis and treatment of diseases are also disclosed.

It is intended to provide a peptide or a phage recognizing nanographite structures and thus enabling efficient recognition, binding, separation and alignment of nanographite structures such as carbon nanohoms or carbon nanotubes, an artificial protein or a chimeric molecule comprising the above-described peptide bonded to a functional peptide, a protein, a labeling, etc., and a complex of the above-described peptide molecule, artificial protein or chimeric molecule with a nanographite structure. By panning peptide-presenting phages bonded to nanographite structures, a nanographite structure-binding peptide capable of specifically recognizing nanographite structures such as carbon nanohoms or carbon nanotubes is obtained.

The product of the invention is a modified form of a therapeutic agent and comprises a therapeutic agent, an oligopeptide having a plasmin peptide substrate of 2-4 amino acids and mono- or di-peptide linkage, a stabilizing group and, optionally, a linker group. The prodrug is cleavable by plasmin. Also disclosed are methods of making and using the prodrug compounds.

Disclosed are methods for producing fusion proteins with the heterodimeric cytokine, interleukin-12. In order to insure that the proper ratio of fused and non-fused subunits are obtained in the fusion protein, a specific stepwise approach to genetic engineering is used. This consists of first expressing the non-fused p40 IL-12 subunit in a production cell line, followed by or simultaneously expressing in the same cell, a second recombinant fusion protein consisting of the fused polypeptide linked by a peptide bond to the p35 subunit of IL-12. Molecules containing the p35 fusion protein cannot be secreted from the transfected mammalian cell without first complexing in a one to one ratio with the p40 subunit, thus ensuring the production of active heterodimeric fusion proteins.

The present invention relates to compounds of the Formula (I), wherein X₁ and X₂ independently represent O, NH or S; R₁ and R₂ are independently selected from the group consisting of a C1-C30 hydrocarbyl group, an amino acid bonded via an amide bond or a peptide bonded via an amide bond each having up to 200 amino acids, the conjugated residue X₁ or X₂ in this case being NH, and hydrogen, both radicals R₁ and R₂ preferably not being H; and R₃ is a residue selected from group consisting of —S—R₆, wherein R₆ is a C1-C30 hydrocarbyl group, at least one of R₁ and R₂ not being H when X₁ and X₂ are oxygen, —S—CH₂—CH(NH₂)(COOH) (cysteine-S-yl), a homologue or derivative (e.g. N-acetyl cysteine-S-yl) thereof, a peptide having up to 200 amino acids which contains at least one amino acid radical with a thiol group, preferably a cysteine radical, and is bonded via the thio sulfur, preferably via the cysteine sulfur (peptide-S-yl), coenzyme A which is bonded via a thiol group or fragments thereof, acyl carrier protein bonded via a thiol group, and dihydrolipoic acid bonded via a thiol group; and pharmaceutically acceptable salts thereof. The present invention also relates to the use of these compounds for preparing a drug, drugs containing the same and their use for the therapy of diseases such as autoimmune disease, NF-kappaB mediated diseases, psoriasis, psoriatic arthritis, neurodermitis, enteris regionalis Crohn, cardiac insufficiency, chronic obstructive pulmonary diseases and asthma and in transplantation medicine.

The present invention provides a chaperonin-target protein complex and a method of producing the same, and a method of stabilizing the target protein, a method of immobilizing the target protein, a method of analyzing the structure of the target protein, a sustained-release formulation, and a method of producing an antibody against the target protein. The chaperonin-target protein complex in the present invention includes a fusion protein having a chaperonin subunit and an affinity tag linked to the chaperonin subunit via a peptide bond and a target protein for which the affinity tag shows a specific affinity, wherein the target protein is bound to the affinity tag by means of the specific affinity, thereby forming a chaperonin ring structure consisting of a plurality of chaperonin subunits. The chaperonin-target protein in the present invention stabilizes the target protein and surely immobilize on a carrier without causing any change in its stereostructure.

The invention relates to a preparation method of fermented rice sticks, which belongs to the field of food fermentation and comprises a pulp making procedure and a pulp steaming procedure. The preparation method of fermented rice sticks is characterized in that 1-1000ml of debranching amylase for per 100kg of rice is added into rice pulp with the weight percent of 5 to 50% before the pulp steaming procedure and after the pulp making procedure, the pH value is regulated to 4.0-5.0, and enzymolysis is carried out for 30-360 min under the temperature of 30-60 DEG C; 1-50 g of protease with proteolysis peptide bonds for per 100kg of rice is added into the rice pulp, and enzymolysis is carried out for 60-360 min under the temperature of 30-60DEG C and the pH value of 4.0-8.0; then, 0.2 ml/kg of zymophyte is added to the pulp and fermented for 360-3600 min under the temperature of 25-30DEG C and the pH value of 5.0-6.0. In the invention, the debranching amylase is utilized to hydrolyze Alpha-D-1, 6 glycoside bonds as the branched chains in starch and dextrine to generate straight-chain compound sugar containing Alpha-D-1, 4 glycoside bonds so as to obtain the effect on debranching and adding straight chains; the protease with proteolysis peptide bonds can be used for hydrolyzing molecular protein and amino acid to provide sufficient amino acid for following mixed fermentation, shorten fermentation time, and improve the quality and the taste of products.

Claimed are a series of somatostatin agonists and uses thereof, according to formula (I),

A¹-cyclo[Cys-A²-D-Trp-A³-A⁴-Cys]-A⁵-Y¹,   (I)

wherein A¹, A², A³, A⁴, A⁵ and Y¹ are as defined in specification provided that the amine nitrogen of at least one peptide bond is substituted with a methyl group or pharmaceutically acceptable salts thereof.

A method of treating chronic hepatitis B is disclosed that comprises administering a T cell-stimulating amount of a vaccine to a patient. The vaccine comprises an immunogenic amount of chimeric, carboxy-terminal truncated hepatitis B virus nucleocapsid (core) protein (HBc) that is engineered for both enhanced stability of self-assembled particles and the substantial absence of nucleic acid binding by those particles. The chimeric protein molecule can include one or more immunogenic epitopes peptide-bonded to one or more of the N-terminus, the immunogenic loop or the C-terminus of HBc. The enhanced stability of self-assembled particles is obtained by the presence of at least one heterologous cysteine residue near one or both of the amino-terminus and carboxy-terminus of the chimer molecule.

The invention relates to an amphipathy triblock polymer. The amphipathy triblock polymer is a linear compound comprising a hydrophilic chain section, an intermediate chain section and a hydrophobic chain section, wherein the hydrophilic chain section is a polyethylene glycol derivative; the intermediate chain section is a peptide polymer comprising lysine, leucine and glutamic acid; the hydrophobic chain section is polyphenylalanine; the polyethylene glycol derivative is connected with one end of the intermediate chain section through an amido bond; and the polyphenylalanine is connected with the other end of the intermediate chain section through a peptide bond. The amphipathy triblock polymer provided by the invention can be self-assembled in an aqueous solution to form a polymer nano-micelle with a three-layer structure; the polymer nano-micelle servicing as a vector has the characteristics of high stability, excellent biocompatibility and the like; the amphipathy triblock polymer is hardly removed by the immune system during circulating in a human body, and hydrophobic small molecule drugs, genetic materials and protein molecules can be effectively loaded at the same time, so that the amphipathy triblock polymer has a wide application prospect. In addition, the invention also provides a preparation method of the amphipathy triblock polymer.

The invention relates to an amphiphilic triblock copolymer, a polymer nano-carrier preparation and preparation methods. The copolymer is a liner polymer compound containing a polyethylene glycol derivative, poly-lysine and poly-leucine, wherein one end of the poly-lysine is connected with the polyethylene glycol derivative through an amide bond, and the other end of the poly-lysine is connected with the poly-leucine through a peptide bond. The amphiphilic triblock copolymer, namely the polyethylene glycol derivative-poly-lysine-poly-leucine triblock copolymer combines the advantages of polyethylene glycol and poly-amino acid, can form a nano-carrier with a three-layer structure by self-assembly in a water solution, and can simultaneously effectively load a small molecular hydrophobic medicament, gene substances and proteins or polypeptides to form a multifunctional nano-carrier.

Rheumatoid arthritis is treated with the administration of a therapeutic dose of a therapeutically acceptable PAD inhibitor. Administration can occur after the onset of RA symptoms, or prophylactically before such symptoms present. In one embodiment, the inhibitor has a side chain including a benzamide group to the left and an ester group to the right of a peptide bond.

The invention contemplates transplacental enzyme replacement therapy (ERT) for deficiency of a polypeptide such as a tissue-nonspecific alkaline phosphatase (TNSALP) by administering a before-described pharmaceutical composition to a pregnant animal whose fetus or embryo is in need of such therapy. The fusion protein of such a composition comprises a water-soluble TNSALP portion, e.g., C-terminus-truncated TNSALP peptide-bonded to an IgG1 antibody Fc portion.

The invention also contemplates a method for treating a metabolic disorder, such as HPP, in a fetus or embryo were a protein is administered to a pregnant mother. The fusion protein comprises a Fc fragment of an IgG1 antibody peptide-bonded to TNSALP. The protein crosses the placenta of the mother and enters the fetal blood stream. The protein is taken up into fetal tissue such that the TNSALP restores normal metabolic activity in the fetus.

The invention relates to the application of the gamma-polyglutamic acid as the absorption promoting agent of the fertilizer in the agricultural husbandry. The said gamma-polyglutamic acid is a high molecular polymer of D-glutacid and L- glutacid formed by the alpha-amino and the gamma-carboxyl in the manner of peptide bond and there is also a dissociative carboxyl on the alpha-carbon atom of every repetitive unit with average molecular weight of 10-10,000 kilodaltons. The said gamma-polyglutamic acid can be used as the absorption promoting agent of the fertilizer made of single element of nitrogen, phosphor, kalium or their composition. The significance of the invention is: it can be degraded by edaphon and its environmental value is much better in relation to TPA; it can reduce by 10-30 % of the normal usage of the chemical fertilizer by applying 10-50 grams of fertilizer per area and increase by 10-30 % of the crop yield, at the same time it can improve the crop quality and increase the crop content of nitrogen, phosphor and kalium.

A KiSS-1 Peptide or a salt thereof can be industrially mass-produced by subjecting a fused protein or peptide in which the KiSS-1 peptide is ligated to the N-terminal of a low-molecular peptide having cysteine at its N-terminal to the cleavage reaction of the peptide linkage on the amino group side of said cysteine residue.