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Biomacromolecule interpenetrating polymer network hydrogel and preparation method thereof

A biomacromolecule and interpenetrating network technology, applied in the field of biomedical polymer materials, can solve the problems of reduced biocompatibility, poor mechanical strength, poor physical cross-linking effect, etc., to overcome toxicity and achieve excellent biocompatibility. Sex, resolving ineffective effects

Inactive Publication Date: 2014-08-27
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Technical problem: In order to overcome the disadvantages of poor mechanical strength of hydrogel and the decrease of cytotoxicity and biocompatibility caused by common chemical cross-linking agents or initiators remaining in the system, the present invention provides a biomacromolecular interpenetrating network hydrogel Gel and its application, the interpenetrating network hydrogel has excellent mechanical properties, good biocompatibility, overcomes the toxicity shortcomings caused by chemical cross-linking agents, and can solve the problem of poor physical cross-linking effect

Method used

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  • Biomacromolecule interpenetrating polymer network hydrogel and preparation method thereof
  • Biomacromolecule interpenetrating polymer network hydrogel and preparation method thereof
  • Biomacromolecule interpenetrating polymer network hydrogel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Preparation of chitosan-p-hydroxyphenylpropionic acid (CHPA)

[0049] Water-soluble chitosan (0.88g, 5mmol) was weighed and dissolved in 150ml of deionized water, and stirred at constant temperature with a magnetic stirrer until completely dissolved. Weigh p-hydroxyphenylpropionic acid (1.66g, 10mmol), 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) (2.73g, 15mmol), N -Hydroxysuccinimide (NHS) (1.72g, 15mmol) was dissolved in 100ml of N,N-dimethylformamide (DMF) aqueous solution, wherein DMF:water (3:2, V:V), activated at room temperature 1h (pH=4.7), the activation solution was added to the chitosan solution, and stirred overnight at room temperature. The reaction solution was moved into the dialysis bag for dialysis with distilled water for 3 days, and the dialysate was adjusted to pH to separate out the product, and vacuum drying was performed to obtain the chitosan of the product bonded p-hydroxyphenylpropionic acid, hereinafter referred to as ...

Embodiment 2

[0051] Preparation of hyaluronic acid-tyramine (HA-Tyr)

[0052] Weigh hyaluronic acid (2g, 5mmol) and dissolve it in 100ml of deionized water, stir with a magnetic stirrer at constant temperature until completely dissolved. Weigh tyramine (1.68g, 10mmol), EDC·HCl (2.73g, 15mmol), NHS (1.72g, 15mmol) dissolved in 100ml of distilled water, activate at room temperature for 1h (pH=4.7), add the activation solution to the transparent acid solution, stirred overnight at room temperature. The reaction solution was transferred into a dialysis bag and dialyzed with distilled water for 3 days, the pH of the dialysate was adjusted to precipitate the product, and the product was vacuum-dried to obtain hyaluronic acid bound to tyramine, hereinafter referred to as hyaluronic acid-tyramine.

Embodiment 3

[0054] Preparation of Heparin-Tyramine (Hep-Tyr)

[0055] Weigh heparin (0.5 g) and dissolve it in 100 ml of deionized water, stir with a magnetic stirrer at constant temperature until completely dissolved. Weigh tyramine (1.68g, 10mmol), EDC·HCl (2.73g, 15mmol), NHS (1.72g, 15mmol) dissolved in 100ml of distilled water, activate at room temperature for 1h (pH=4.7), add the activation solution to the heparin The solution was stirred overnight at room temperature. The reaction solution was transferred into a dialysis bag and dialyzed with distilled water for 3 days, the pH of the dialysate was adjusted to precipitate the product, and the product was vacuum-dried to obtain heparin bound to tyramine, hereinafter referred to as heparin-tyramine.

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Abstract

The invention discloses a biomacromolecule interpenetrating polymer network hydrogel and a preparation method of the biomacromolecule interpenetrating polymer network hydrogel. The biomacromolecule interpenetrating polymer network hydrogel is formed by crosslinking two kinds of enzymes in a catalysis mode, one is a polysaccharide macromolecule network formed by crosslinking polysaccharide macromolecules with introduced phenolic hydroxyl groups in an oxidation mode as oxidase and hydrogen peroxide catalyze phenolic hydroxyl groups, and the other is a protein or polypeptide macromolecule network formed crosslinking protein or polypeptide containing amino acid residues as transferase catalyzes peptide bonds. The two networks interpenetrate each other and form the novel interpenetrating polymer network hydrogel without bonding of chemical bonds. No chemical crosslinking agent is used in the hydrogel, and the hydrogel has excellent biocompatibility and mechanical property, can be shaped like a dry or wet film, like porous sponge or fibers, and can serve as a contact lens, a medicine release carrier, a scaffold for tissue engineering or materials for tissue repair.

Description

technical field [0001] The invention is an interpenetrating network hydrogel formed by cross-linking biomacromolecules catalyzed by two different enzymes and a preparation method thereof, belonging to the field of biomedical macromolecular materials. Background technique [0002] Hydrogel is a three-dimensional network polymer that can swell in water and retain a large amount of water without dissolving in water. It shows good biocompatibility when in contact with blood, body fluids and human tissues. Hydrogel is closer to living tissue than any other synthetic biomaterial, and is similar in nature to the extracellular matrix. After absorbing water, it can reduce friction and mechanical effects on surrounding tissues, and significantly improve the biological properties of the material. Therefore, hydrogels have great application potential in tissue engineering and regenerative medicine. [0003] Interpenetrating network (IPN) hydrogels are polymers with unique properties fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/00C08L89/00C12P19/26C12P19/08C12P19/04C12P21/00A61K47/42A61K47/36A61K9/06A61L27/22A61L27/20A61L27/52
Inventor 李新松张叶敏吴旸范治平
Owner SOUTHEAST UNIV
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