10 results about "Kilodalton" patented technology

The invention is directed to compositions and methods for the stabilization of viral and bacterial vaccines. Vaccines of the invention are contained in VLPs with stabilizing agents such as, for example, sugar alcohols (e.g., sorbitol) and degraded gelatins. Preferably the gelatin has an average molecular weight of 10,000 kilodaltons or less. These vaccines have a substantially improved thermostability as well as long term stability. The invention is also directed to the manufacture of a vaccine or the invention and methods for the administration of a vaccine of the invention to patients.

This invention provides methods for quantitatively determining the concentration of free iron and ferritin-bound iron in a sample. The method includes diluting a ferritin-containing sample with a diluent to create a slurry, processing the slurry through a crossflow filtration system equipped with a membrane of at least 500 kilodaltons molecular weight cutoff, collecting the permeate containing free iron and the retentate containing ferritin-bound iron analyzing the iron concentrations in the permeate and retentate using a suitable analytical technique and calculating the mass balance of the total iron content. The invention also provides an antibody for use in quantitating the amount of ferritin in a sample and methods of treating iron deficiency in a patient in need thereof.

Agents that are less than 100 kilodaltons, bind a membranal immune receptor on a surface of a cell and inhibit proteolytic cleavage of the immune receptor are provided. Methods of treating cancer and improving immunotherapy comprising administering the agents are also provided.

The application belongs to the technical field of biological medicine, and particularly relates to a nano antibody against beta-catenin and a preparation method and application thereof. Specifically, the nano antibody with excellent affinity to beta-catenin is obtained through sample screening, has the advantages of small molecular weight (about 15 kilodaltons), small immunogenicity, better solubility and stability, and a long CDR3 region, thereby being beneficial to basic research and clinical experiments, having potential tumor diagnosis and treatment prospects, and thus having good practical application value.

The invention relates to the technical field of solid tumor clinical diagnosis and treatment, and discloses a cell membrane protein pump efficiency monitoring system for solid tumor brain metastasis risk assessment. Comprising a biological sample processing module used for obtaining solid tumor biological sample cells, preparing biological sample cells to be detected and obtaining positive control sample cells; the image acquisition module is used for performing protein detection on the to-be-detected biological sample cells and the positive control sample cells to obtain a detection image; the fractional enhanced image learning module is used for carrying out third-order fractional enhanced image learning on the detection image, and the third-order fractional enhanced image learning comprises the following steps: carrying out secondary quantitative analysis on P-glycoprotein of a biological sample cell to be detected on a predicted 141 thousand Dalton site in the detection image; performing third feature image learning on the detection image in a fuzzy towing region appearing at a 150-300 thousand Dalton position on the membrane in the detection image; and the risk assessment module is used for comprehensively assessing the brain metastasis risk of the solid tumor based on the result of the third-order fractional enhanced image learning.

This invention provides methods for quantitatively determining the concentration of free iron and ferritin-bound iron in a sample. The method includes diluting a ferritin-containing sample with a diluent to create a slurry, processing the slurry through a crossflow filtration system equipped with a membrane of at least 500 kilodaltons molecular weight cutoff, collecting the permeate containing free iron and the retentate containing ferritin-bound iron analyzing the iron concentrations in the permeate and retentate using a suitable analytical technique and calculating the mass balance of the total iron content. The invention also provides an antibody for use in quantitating the amount of ferritin in a sample and methods of treating iron deficiency in a patient in need thereof.

The invention discloses a preparation method of a salty-taste-enhanced ferrous chelate special for low-sodium food, and belongs to the technical field of food nutrition enhancement and vegetable protein deep processing. Red bean protein serves as a raw material, salty-taste-enhanced red bean protein zymolyte with the molecular weight ranging from one thousand Daltons to three thousand Daltons and rich in glutamic acid and aspartic acid is prepared through directional enzymolysis of alkaline protease, a stable three-dimensional cross-linking chelation system is constructed with carboxyl bidentate coordination as a core mode, and the salty-taste-enhanced red bean protein zymolyte with the molecular weight ranging from one thousand Daltons to three thousand Daltons is obtained. And preparing to obtain the difunctional ferrous chelate. Under the optimal process, the ferrous ion chelation rate reaches 67.49%, the chelate has excellent stability under the conditions of weak acidity, high salt and hot processing, metal peculiar smell of ferrous ions can be effectively masked, meanwhile, the salty taste and umami taste characteristics of red bean peptide are reserved, the double functions of iron nutrition enhancement and salty taste enhancement are achieved, and the chelate is suitable for being used in the field of food processing. And a technical support is provided for high-value utilization of the red bean protein and development of a novel bifunctional iron enhancer.

The application relates to a zinc-containing medical device, which comprises a zinc-containing base body and a polylactic acid coating arranged on the zinc-containing base body, wherein the thickness of the polylactic acid coating is x micrometers, and x and the weight average molecular weight Mn kilodaltons of the polylactic acid satisfy the formula: the corrosion rate of zinc in the base body is small, the mechanical properties can be maintained for a sufficient repair period, and the biological risk is low. When the polylactic acid is poly-racemic lactic acid, a=0.0336ln(Mn)-0.1449, b=-0.472ln(Mn)+2.1524, and c=1.1604ln(Mn)-5.7128; when the polylactic acid is poly-lactic acid, a=-0.006ln(Mn)+0.03441, b=0.0648ln(Mn)-0.3662, and c=-0.162ln(Mn)+0.7847.

This invention provides methods for quantitatively determining the concentration of free iron and ferritin-bound iron in a sample. The method includes diluting a ferritin- containing sample with a diluent to create a slurry, processing the slurry through a crossflow filtration system equipped with a membrane of at least 500 kilodaltons molecular weight cutoff, collecting the permeate containing free iron and the retentate containing ferritin-bound iron analyzing the iron concentrations in the permeate and retentate using a suitable analytical technique and calculating the mass balance of the total iron content. The invention also provides an antibody for use in quantitating the amount of ferritin in a sample and methods of treating iron deficiency in a patient in need thereof.

Agents that are less than 100 kilodaltons, bind a membranal immune receptor on a surface of a cell and inhibit proteolytic cleavage of the immune receptor are provided. Methods of treating cancer and improving immunotherapy comprising administering the agents are also provided.