191results about How to "Optimization order" patented technology

The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries. In several aspects of the present invention, there are methods based on methylation-dependent enrichment or depletion of genomic DNA isolated from cellular and cell-free sources. In additional embodiments, there are methods and compositions for single-step high throughput preparations of Methylome libraries.

The present invention provides methods for the sequencing of all RNA species within an RNA sample, such as the RNA content obtained from a cell, a tissue, a living organism, or from an artificial source. RNA molecules within the samples are labeled in a RNA-specific manner prior to immobilization on a solid support. One label is used to mark the location of the RNA molecule on the solid support, whereas the second label is used to mark selectively the S′ end of full-length mRNA molecules. RNA molecules are sequenced while being bound to the solid support in one or more sequencing reactions, and sequences of individual RNA molecules can be forwarded to computational analysis for assembling sequence information from individual sequencing reads obtained from the same location on the solid support. Not only unsupervised expression profiling on a genome-wide scale, but also the direct analysis of RNA-RNA interactions become possible as revealed by the analysis of the sequencing information obtained along with genomic information.

This invention relates generally to the targeting and biopsy of tissue for medical purposes, and more particularly to a targeted biopsy system which allows planning of tissue to be sampled, targeting of specific areas of tissue in reference to the plan, capturing the tissue sample and recording the source location of the tissue sample, particularly for use in collecting tissue samples from the prostate gland. A further purpose of this invention is to provide a targeted treatment system which allows planning of tissue to be treated, targeting of specific areas of tissue in reference to the plan, and delivering the treatment to the targeted tissue.

Aspects of the invention relates to methods and compositions that are useful to reduce bias and increase the reproducibility of multiplex analysis of genetic loci. In some configurations, predetermined preparative steps and / or nucleic acid sequence analysis techniques are used in multiplex analyses for a plurality of genetic loci in a plurality of samples.

The present invention relates to methods and systems for the exhibition of a motion picture with enhanced perceived resolution and visual quality. The enhancement of perceived resolution is achieved both spatially and temporally. Spatial resolution enhancement creates image details using both temporal-based methods and learning-based methods. Temporal resolution enhancement creates synthesized new image frames that enable a motion picture to be displayed at a higher frame rate. The digitally enhanced motion picture is to be exhibited using a projection system or a display device that supports a higher frame rate and / or a higher display resolution than what is required for the original motion picture.

A method for retrofitting DNA in a single-copy or high-copy vector, such as a fosmid or BAC, whereby an artificial transposon is used to introduce a conditional multi-copy origin of replication (“ori”) into the DNA in said vector. Following random in vitro or in vivo transposition of the ori-containing transposon into DNA in the single-copy or low-copy vector, the resulting insertion clones are introduced into a special host strain that contains a gene which encodes a polypeptide required for replication from the multi-copy ori. However, since the gene for this polypeptide is expressed from a tightly-regulated inducible promoter, the polypeptide is not expressed in the absence of inducer. On addition of inducer to the culture medium, the host cell synthesizes the polypeptide, which in turn activates replication from the multi-copy ori, thereby increasing the amount of clone DNA synthesized by the cell.

The present invention includes novel compositions and method for nucleic acid sequencing. The methods and compositions permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number of different oligonucleotides with superior output and quality.

A system and method for using magnetic resonance to track a medical device which attenuates interfering MR signals. The system comprises an MR tracking device comprising one or more radiofrequency (RF) tracking coils adapted to receive a magnetic resonance MR response signal from nuclei excited by an RF pulse. Ideally, the RF tracking coil only receives an MR response signal from nuclei in matter in the close vicinity of the tracking device. The tracking device is attached to medical device. The interfering signal tends to emanate from large objects or large volumes that are outside the close vicinity of the tracking device but which are still weakly coupled to the tracking device. A dephasing gradient is applied perpendicular to the readout gradient before the RF response signal is received thereby dephasing the interfering signal emanating from remote nuclei, which strongly attenuates the interfering signal while the MR response signal is substantially unaffected. The system can also detect errors in tracking location by checking the amplitude of the signal from each coil and the detected distance between each coil, and correcting for such errors by ignoring data from coils having low amplitude or location deviating from known location relative to the other coils.

Disclosed are compositions and methods for amplification of RNA molecules. The disclosed method involves synthesizing first strand cDNA molecules from RNA molecules, circularizing the first strand cDNA molecules and replicating the circularized first strand cDNA molecules using rolling circle replication. The method can be aided by the use of specialized primers for cDNA synthesis and specialized probes for circularizing the first strand cDNA molecules. The method can be used to replicate and amplify multiple RNA molecules, such as all RNA molecules in a sample or all mRNA molecules in a sample, or be used to replicate and amplify specific RNA molecules. Rolling circle replication of the circularized first strand cDNA molecules results in long DNA strands containing tandem repeats of the cDNA sequence. The tandem sequence DNA can be used directly (for detection of sequences, for example), further amplified, or used any other purpose. Double-stranded tandem sequence DNA can be used to produce unit lengths of the cDNA sequence. Tandem sequence DNA can also be transcribed to produce transcripts having sequence complementary to or matching the sequence of RNA molecules.

The invention relates to a method for sequencing a heteropolymeric target nucleic acid sequence that involves stochastic sensing. The invention also relates to a method for improving a pore for sequencing a target nucleic acid sequence by modifying one or more sites in the pore.

The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.

The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.

The invention relates to a substantially pure thermostable DNA polymerase from Thermotoga (Tne and Tma) and mutants thereof. The Tne DNA polymerase has a molecular weight of about 100 kilodaltons and is more thermostable than Taq DNA polymerase. The mutant DNA polymerase has at least one mutation selected from the group consisting of (1) a first mutation that substantially reduces or eliminates 3'->5' exonuclease activity of said DNA polymerase; (2) a second mutation that substantially reduces or eliminates 5'->3' exonuclease activity of said DNA polymerase; (3) a third mutation in the O helix of said DNA polymerase resulting in said DNA polymerase becoming non-discriminating against dideoxynucleotides. The present invention also relates to the cloning and expression of the wild type or mutant DNA polymerases in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes. The DNA polymerases of the invention may be used in well-known DNA sequencing and amplification reactions.

A speech recognition system includes an input device to receive voice sounds, one or more processors, and one or more storage devices storing parameters and program modules including instructions which cause the one or more processors to perform operations. The operations include extracting an acoustic feature sequence from audio waveform data converted from the voice sounds, encoding the acoustic feature sequence into a hidden vector sequence using an encoder network having encoder network parameters, predicting first output label sequence probabilities by feeding the hidden vector sequence to a decoder network having decoder network parameters, predicting second output level sequence probabilities by a hybrid network using character-base language models (LMs) and word-level LMs; and searching, using a label sequence search module, for an output label sequence having a highest sequence probability by combining the first and second output label sequence probabilities provided from the decoder network and the hybrid network.

Methods of characterizing a nucleic acid library by digital assay.

Methods and systems of DNA sequencing that compensate for sources of noise in next-generation DNA sequencers are described.

The present invention relates to recombinant bovine parainfluenza virus (bPIV) cDNA or RNA which may be used to express heterologous gene products in appropriate host cell systems and / or to rescue negative strand RNA recombinant viruses that express, package, and / or present the heterologous gene product. In particular, the heterologous gene products include gene product of another species of PIV or from another negative strand RNA virus, including but not limited to, influenza virus, respiratory syncytial virus, human metapneumovirus and avian pneumovirus. The chimeric viruses and expression products may advantageously be used in vaccine formulations including vaccines against a broad range of pathogens and antigens.

Some embodiments provide a method of combining multiple streams of data packets into a single combined stream in a manner that facilitates accurate estimation of bandwidth of a connection over a network between two devices. When combining the streams into the combined stream, the method associates a set of packets from a first stream and a reference packet from a second stream to form a longer sequence of packets in the combined stream. The method sends the combined stream from a first device to a second device so that the second device can estimate the bandwidth of the connection between the first and second devices based on the inter-arrival times of the packets in the sequence of packets.

A method of scheduling a plurality of tasks for a plurality of memories in a memory system is disclosed. The method includes classifying each task among the plurality of tasks to a task type among a plurality of task types, disposing a plurality of task queues according to the plurality of task types wherein each task queue stores tasks to be executed within the plurality of tasks, assigning a priority for each task type among the plurality of task types, disposing at least one execution queue; and converting a first task stored in a first task queue among the plurality of task queues into at least one command to be stored in a first execution queue among the at least one execution queue, wherein the at least one command is executed according to the priority of a first task type corresponding to the first task queue.

The present invention provides a method for amplifying a target sequence while suppressing inhibition of an amplification reaction in nucleic acid amplification in the presence of the probe. At the time of amplifying the target sequence, as the probe caused to coexist in amplification of the target sequence, a probe having a base sequence in which a melting temperature of the double-stranded nucleic acid is equal to or lower than a reaction temperature of the elongation reaction is used. In the presence of such a probe, for example, annealing of a primer and an elongation reaction from the primer are hardly inhibited by the presence of the probe so that amplification of the target sequence can be conducted sufficiently. Therefore, when a polymorphism of a target site in the target sequence is analyzed by a Tm analysis or the like, high reliability can be achieved.

A simple instruction set processor preferably utilizes six primary components: a fetch unit, and instruction and address register, a controller / decoder, an arithmetic logic unit, an address multiplexer, and a storage multiplexer. The processor utilizes a data stream containing within it the address for a subsequent instruction to be executed by the processor, thereby avoiding the need for registers of the type utilized in prior art processors. As a result, the processor utilizes a minimal number of registers to perform its operations. The processor utilizes an instruction set in which every instruction contains a JUMP to the next instruction. By utilizing JUMPs in every instruction and providing the address to which the processor is to JUMP, there is no need for address counters and register pointers. Also, extremely fast state changes are facilitated the contents of only one register identifying a next address must be saved or restored. By eliminating data registers, data streams of any width may be supported by suitably utilizing a plurality of processor connected in parallel. The elimination of multiple registers enables the processor to more easily be embedded within memory arrays themselves. The processor preferably utilizes six primary components: a fetch unit, and instruction and address register, a controller / decoder, an arithmetic logic unit, an address multiplexer, and a storage multiplexer.

A method and system are provided for controlling a lock state of a charging cable connected to a power inlet of an electrical vehicle. The vehicle includes an energy storage device electrically connected to the power inlet, a lock unit for locking the charging cable to the power inlet, and a control unit for controlling a lock state of the lock unit.

This is a self-contained disposable thermal cycler device in which target sequence is amplified as the samples passes over a grid of alternating temperature and then at the completion of the reaction, the amplified material is captured by probes complementary to the targeted sequence. Since the device can be sealed and is disposable, it reduces the occurrence of cross contamination or specimen carry-over.

The invention relates to a viewing system and a method for detecting an object of interest in a sequence of images (IS). Said object of interest is detected by first locating localizers related to said object of interest and by locating borders (BL) related to said object of interest using the location (LI, LZ) of said localizers. The viewing system according to the invention is able to produce a sequence of enhanced images in which the object of interest is enhanced, to measure some characteristics and to build a three dimensional representation of said object of interest. The viewing system is also able to register and combine said sequence of enhanced images with a sequence of reference images.

The invention relates to a wind farm reactive comprehensive optimization control method. The method comprises the following steps of: inputting signals into a wind farm reactive comprehensive optimization control system; switching plant or wind farm grid combining point capacitors and reactors, controlling a static var compensator (SVC) and controlling wind farm units by using the wind farm reactive comprehensive optimization control system. Through the scheme, the automatic control level of wind farm scheduling, the utilization ratio of reactive configuration equipment and the voltage stabilization level of a power grid can be improved, and reactive power comprehensive optimization coordination control over wind farms is realized.

Methods and systems for image and video enhancement and data-loss recovery using wavelet transforms are disclosed. A wavelet transformed image is defined as multiple sections, where one section is the original image, and coefficients are estimated for each other section using information obtained from that section. An inverse wavelet transform on the wavelet transformed image obtains an enlarged image. For obtaining a high resolution video frame, one section is the original video frame, and coefficients are estimated for each other section using information from a lower level wavelet transform of a corresponding section of a past or future frame of the video sequence. For recovery of data lost from transmitted original data, the locality of the lost data is determined in lower and higher levels of a wavelet transform tree, and information at the locality in the lower and higher levels is used to estimate the lost data.

A method is provided for the simultaneous detection of human papillomavirus DNA and RNA in a sample.