307 results about "Direct analysis" patented technology

The present invention provides biochips for ion transport measurement, ion transport measuring devices that comprise biochips, and methods of using ion transport measuring devices and biochips that allow for the direct analysis of ion transport functions or properties. The present invention provides biochips, devices, apparatuses, and methods that allow for automated detection of ion transport functions or properties. The present invention also provides methods of making biochips and devices for ion transport measurement that reduce the cost and increase the efficiency of manufacture, as well as improve the performance of the biochips and devices. These biochips and devices are particularly appropriate for automating the detection of ion transport functions or properties, particularly for screening purposes.

The present invention provides methods for the sequencing of all RNA species within an RNA sample, such as the RNA content obtained from a cell, a tissue, a living organism, or from an artificial source. RNA molecules within the samples are labeled in a RNA-specific manner prior to immobilization on a solid support. One label is used to mark the location of the RNA molecule on the solid support, whereas the second label is used to mark selectively the S′ end of full-length mRNA molecules. RNA molecules are sequenced while being bound to the solid support in one or more sequencing reactions, and sequences of individual RNA molecules can be forwarded to computational analysis for assembling sequence information from individual sequencing reads obtained from the same location on the solid support. Not only unsupervised expression profiling on a genome-wide scale, but also the direct analysis of RNA-RNA interactions become possible as revealed by the analysis of the sequencing information obtained along with genomic information.

The present invention recognizes that the determination of ion transport function or properties using direct detection methods, such as whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and other fluidic components and methods of use that allow for the direct analysis of ion transport function or properties using microfabricated structures that can allow for automated detection of ion transport function or properties. These biochips and fluidic components and methods of use thereof are particularly appropriate for automating the detection of ion transport function or properties, particularly for screening purposes.

The invention relates to a method for planning a path for a mobile robot based on environmental modeling and a self-adapting window, which relates to a method for planning a real-time path for the mobile robot. The method comprises the following steps of: performing modeling and analysis on a multiple constraint local environment; performing passable analysis; performing safety analysis; performing motion smoothness analysis; performing goal-directed analysis; and performing path planning by adopting the self-adapting window. Because the method has better environmental suitability and obstacle avoiding capacity, the method obtains good safety and reachability, has high calculation real-time property, so the method solves the problem that the mobile robot generates an obstacle avoidance path in real time in an uncertain complex environment, provides a path selection method with optimized integration, better meets the requirements on obstacle avoidance for the mobile robot, realizes the real-time path planning and control of the robot, and provides an effective collision-less path planning method for the navigation application of the mobile robot.

This invention relates to a desorpton / ionization source operated under ambient conditions for direct analysis of solid or liquid samples on a surface. The source comprises of a laser desorption system and a UV / electrospray combined ionization system. The source is suitable for simultaneously ionizing samples with different polarity in a complex mixture. At the same time, the compact design of the source with multiple channels can maintain the level of local concentration of the analyte ions inside the source for higher efficiency of sample ionization and introduction.

An apparatus and method for direct analysis of continuous-flow liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS) including a sample stage that is adapted to receive a liquid sample and a nebulizing ionizer that is configured to generate a charged, nebulized solvent and thereby desorb at least a portion of the liquid sample from the sample stage.

In the past, the practice of creating investment portfolio has been focused on the diversification of investment without specifically identifying investment risks. This invention is related to a method of diversification which considers the level of potential for conflict of interest (COI) risk as part of a diversification process. The COI has its origin in the interaction among market participants, market structure, business arrangement and practice. The advantage of the present invention is that the COI becomes a primary factor in creating the portfolio. This method provides a direct analysis of the COI risk that associates with entire investment process and the manager, and it is further integrated into the portfolio creation process. The COI Method opens new paths to perform risk analysis and could be integrated into decision process in other fields of investment.

The invention discloses an online monitoring and fault diagnosis system of large wind turbine units. A sensor and data collection equipment are installed on a wind turbine unit to be detected, so as to collect equipment operation state characteristic signals, such as video signals, noise signals, flame signals, electric signals, vibration signals and pressure signals, of the wind turbine unit, and transmits the state characteristic signals to a central control room of a wind power plant through a network in real time; and operation and maintenance personnel can know the operation state of the wind turbine unit at any time in the central control room of the wind power plant, and can directly analyze signal data of the abnormally operated wind turbine unit, so that operation conditions of gear boxes, main shafts, generators, bearings, hydraulic systems, yaw systems, bearing lubricating systems, brake systems, blades and towers of all units in the wind power plant are monitored in real time and failure warning information is output. Data can also be transmitted to a remote fault diagnosis center through a network, a project team performs professional analysis to faults and issues a diagnosis report, and a field maintenance team performs corresponding repair / maintenance operation.

The present invention provides a device, system, and associated methods to actively or passively sample air by directing it onto the surface of a porous light-absorbing semiconductor, for example, a desorption / ionization on porous silicon (“DIOS”) chip. Upon adsorption of an analyte, the surface may be analyzed directly by laser desorption / ionization time-of-flight mass spectrometry. Because the process of laser desorption / ionization and subsequent mass detection does not require elevated temperatures, thermal degradation of analytes is avoided.

The present invention provides a device, system, and associated methods to actively or passively sample air by directing it onto the surface of a porous light-absorbing semiconductor, for example, a desorption / ionization on porous silicon (“DIOS”) chip. Upon adsorption of an analyte, the surface may be analyzed directly by laser desorption / ionization time-of-flight mass spectrometry. Because the process of laser desorption / ionization and subsequent mass detection does not require elevated temperatures, thermal degradation of analytes is avoided.

A method the direct analysis of an analyte in keratinized structures, e.g., hair and fingernails, which comprises preparing a mixture containing a low redox potential activator compound such as dithiothreitol or dithioerythritol, an enzyme suitable for the digestion of the keratin structure, a sample of the keratin structure and a biological detergent that aids the digestion of the keratinized structure at a relatively low pH, e.g., between about 6.2 and 8; permitting the enzyme to at least substantially digest the sample of keratin structure, and subjecting the digest solution to analysis, preferably by radioimmunoassay, to determine the identity and amount of analyte in the keratin structure sample. To accelerate the method, cupric sulfate may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention. The preferred biological detergents include betaine, sulfo-betaine, alkylglucosides and bile acids.

The present invention discloses the method and system of processing MDX multi-dimensional data inquiry statement. The present invention includes for the user to send MDX multi-dimensional inquiry task to the inquiry treatment subsystem; for the inquiry treatment subsystem to read the MDX multi-dimensional inquiry task, to analyze directly, to optimize serially and parallelly and to decompose the MDX multi-dimensional inquiry task into several son inquiry tasks; for the inquiry treatment subsystem to send the son inquiry plan to corresponding data warehouse servers; for the data warehouse servers to execute corresponding inquiry plan and to return the inquiry result to the inquiry treatment subsystem; and for the inquiry treatment subsystem to synthesize the inquiry results and to return to the user. The present invention realizes the direct analysis and treatment of MDX language, has high inquiry efficiency, and is especially suitable for multi-dimensional data inquiry.

An apparatus and method for direct analysis of continuous-flow liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS) including a sample stage that is adapted to receive a liquid sample and a nebulizing ionizer that is configured to generate a charged, nebulized solvent and thereby desorb at least a portion of the liquid sample from the sample stage.

Devices and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.

A system using tank corrosion sensors to provide for an overall assessment and monitoring of the electro-chemical corrosion and coatings condition in ships' tanks, and particularly in ships' seawater or compensated fuel tanks. The system includes reference half-cells mounted along a suspended cable and one instrumented sacrificial anode at the end of the cable to provide optimal sensing capability within a tank structure. The reference half-cells and the sacrificial anode measure a potential and current output, respectively. Together the measurements provide objective information that can be used to predict corrosion damage and coating deterioration occurring throughout the structure of the tank. The system may be used for an overall assessment and monitoring of the electrochemical corrosion and coatings condition. In a preferred embodiment, the measurements are stored in a datalogger that is optimally contained within an associated instrument housing. If used with other systems in other tanks, the system may be used to monitor the relative tank condition, trend tank condition changes over time, range tank behavior into three categories and provide a direct analysis methodology for making tank maintenance decisions.

An extraction and analysis device includes a microfluidic based collection system that extracts one or more different analytes from a fluid-based sample and an optical analysis system directly coupled to the collection system to perform optical analysis on the one or more collected analytes. The microfluidic based collection system includes microfluidic circuitry for directing a fluid based sample to a purification chip. Analytes collected within the purification chip can be either subsequently removed and analyzed or the analytes can be analyzed directly, while still within the purification chip, using the optical analysis system. The purification chip is preferably comprised of a plurality of pillars, the surface area of each pillar is coated with a specific capture chemistry. The specific capture chemistry is applied by derivitizing the pillars such that a ligand, such as a nucleic acid, an amptimer, or an antibody is attached to each pillar.

The invention discloses a method for identifying damages of a frame structure. The method comprises the following steps: firstly, testing an undamaged frame structure, and carrying out model modification on an initial discretization model of the frame structure so as to obtain an accurate finite element theoretical model, and calculating a structural modal parameter (omega A0, phi A0); then, testing the damaged frame structure, and carrying out model analysis on the damaged frame structure so as to obtain a modal parameter (omega t2, phi t2); and finally, establishing a direct analysis method damage identification equation, and solving an unit damage coefficient Di (i=1, 2,..., n) by using front n (n is less than or equal to N) order frequencies and vibration mode variation value delta omega r (r=1, 2,..., n) of the frame structure before and after damage so as to achieve the purpose of simultaneously identifying the number, position and size of the damages existing in the whole structure. The method is suitable for the damage identification of a construction frame structure, and has the advantages of less required detection data amount, high efficiency and higher precision.

The invention discloses a vehicle-mounted dust-haze pollutant real-time on-line monitoring system and a vehicle-mounted dust-haze pollutant real-time on-line monitoring method. The monitoring system includes a PM2.5 online mass spectrum detection system, a VOCs online mass spectrum detection system and a VOCs online spectrum detection system which are arranged on a moving monitoring vehicle. The monitoring method includes the steps of performing on-line measurement to particulate matters in atmosphere through PM2.5 online time of flight mass spectrum to obtain number concentration of the particulate matters, spectrogram of particle size and online mass spectrum data, classifying the ingredients of the particle matters through an ART-2a method to obtain chemical ingredient data of single particle under different time resolutions and particle sizes, analyzing the source of the particulate matters through a mass spectrum direct analysis technology to determine the source of the particulate matters, directly performing online measurement to VOCs in atmosphere through the VOCs online time of flight mass spectrum to obtain the ingredient and concentration characters of the VOCs, and directly performing online measurement to the VOCs in atmosphere through VOCs online spectrum to obtain the ingredient and concentration characters of the VOCs.

The current invention involves a method and a device for generating and analyzing ions in order to analyze samples directly without sample preparation. The gaseous neutral molecules are desorbed under atmospheric pressure by a desorption method. The desorbed neutral molecules are then transferred into a low pressure region where they are post-ionized by a mist from an electrospray probe tip or by photons from a vacuum UV source. The generated ions are then focused in a time varying electric field in the low pressure chamber before they are transferred into a mass spectrometer or ion mobility spectrometer for further analysis.

A method and apparatus for automated testing of display signals from video graphics circuitry includes capturing display signals that are provided from a processing device to the display device. The method and apparatus further includes converting the display signals into data acquisition signals, where a data acquisition signal includes a converted display signal having the display information contained therein wherein the data acquisition signal is in a form capable of being directly analyzed by a testing system. Furthermore, the method and apparatus includes providing the data acquisition signals to a test system that tests the display signals.

A card or insert having a plurality of recesses for a sample preparation device, the card containing cast-in-place composite and / or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular card size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a plurality of recesses in an insert for a multi-well sample preparation device, thereby providing an effective platform for high throughput micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted in multiplicity, including selective bind / elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. Manufacturing flexibility and high throughput is achieved. The card can be configured for direct analysis of bound sample without elution.

An extraction and analysis device includes a microfluidic based collection system that extracts one or more different analytes from a fluid-based sample and an optical analysis system directly coupled to the collection system to perform optical analysis on the one or more collected analytes. The microfluidic based collection system includes microfluidic circuitry for directing a fluid based sample to a purification chip. Analytes collected within the purification chip can be either subsequently removed and analyzed or the analytes can be analyzed directly, while still within the purification chip, using the optical analysis system. The purification chip is preferably comprised of a plurality of pillars, the surface area of each pillar is coated with a specific capture chemistry. The specific capture chemistry is applied by derivitizing the pillars such that a ligand, such as a nucleic acid, an amptimer, or an antibody is attached to each pillar.

The disclosure provides a method of predicting resistance to androgen receptor (AR) targeted therapy in a prostate cancer patient comprising (a) performing a direct analysis comprising immunofluorescent staining and morphological characterization of nucleated cells in a blood sample obtained from the patient to identify circulating tumor cells (CTCs), and (b) based on said direct analysis further determining the presence of a biomarker signature that is predictive of resistance to AR targeted therapy in the prostate cancer patient, wherein the biomarker signature comprises CK+, AR+, nucleoli+ CTCs in a subpopulation of said CTCs. The present disclosure also provides a method of predicting resistance to taxane-based chemotherapy in a prostate cancer patient comprising (a) performing a direct analysis comprising immunofluorescent staining and morphological characterization of nucleated cells in a blood sample obtained from the patient to identify circulating tumor cells (CTCs), and (b) based on said direct analysis further determining the presence of a biomarker signature that is predictive of resistance to taxane-based chemotherapy in the prostate cancer patient, wherein the biomarker signature comprises CK+, AR−, nucleoli+, small size in a subpopulation of said CTCs.

The invention discloses a direct analysis device of solid samples, wherein a sample pool is installed on a three-dimensional moving platform, a microscopic imaging system is aligned to a solid sample in the sample pool, a laser beam emitted by a picosecond laser is focused onto the surface of the sample in the sample pool through a laser optical system, particles generated by laser ablation enter an inductively coupled plasma source mass spectrometer through an aerosol transmission system, the direct analysis device has the advantages that the laser wavelength can be selected from 1064 nm, 532 nm, 355 nm and 266 nm, the pulse width is 30 ps, the pulse frequency is 20 Hz, the pulse energy is continuously adjustable, and the maximal pulse energy of 266 nm reaches 3.0 mJ; the position of the sample pool is three-dimensional controllable, and the transmission pipeline of the aerosol transmission system switched by a tee solenoid valve is short; a non-coaxial system is adopted to quickly focus and position the sample ablation position. As shown in analysis, basically no element fractionation effect exists in the elements of low alloy steel, pure copper, pure aluminum and the like, and basically no matrix effect exists in the elements of iron base, copper base, aluminum base and the like.

The invention provides a branched boric acid functionalized monolithic column which is a flexible branched boric acid functionalized monolithic column obtained by bonding flexible branched macromolecules onto the hole surface of a base monolithic column through the replacement of boric acid functionalization. The invention further provides a preparation method of the branched boric acid functionalized monolithic column and the application thereof in separation, enrichment and purification of biomolecules containing cis-dyhydroxyl structures. According to the branched boric acid functionalized monolithic column and the preparation method and the application thereof, the monolithic column is high in affinity and capacity, wide in adaptive PH range, high in capacity of resisting disturbance and being suitable for direct analysis of practical samples without PH adjustment.

The invention discloses a method for preparing a micro-flow chip. The method comprises the following step of preparing and operating a masks, namely, designing two masks, wherein the masks comprise light-sensitive areas and non-light-sensitive areas; the light-sensitive area of a first mask comprises a first sealing graph and a banding graph which is isolated from the first sealing graph for a certain distance; the light-sensitive area of a second mask comprises a second sealing graph and a banding graph; the second sealing graph is formed in a mode that the first sealing graph is expanded in parallel till being connected with the banding graph; and the method further comprises a step of carrying out first etching operation, second etching operation and bonding operation. By utilizing the chips prepared by using the method disclosed by the invention, tumor cells can be effectively separated, and moreover the tumor cells after being captured can be directly analyzed.

A liquid chromatograph has switching valves as a flow passage switching mechanism and an automatic sampler as an injection section. The liquid chromatograph can switch, by switching the switching valves between a concentration flow passage for concentrating the sample injected by the automatic sample via a flow passage which connects the automatic sampler to the trapping column; a concentration analysis flow passage for separating and analyzing the concentrated sample on a per-composition basis which connects the trapping column to the analysis column; and a direct analysis flow passage by separating the sample on a per-composition basis which connects the automatic sampler to the analysis column.

The invention belongs to the gas-sensitive analysis system field and relates to an external temperature control type intelligent gas-sensitive analysis device. The device is characterized in that the device can realize direct analysis on the gas-sensitive properties of materials and realize independence of material gas-sensitive property research from sensor devices. The external temperature control type intelligent gas-sensitive analysis device provided by the invention comprises a test platform (1), a gas chamber (2), a control system (3), a cooling water circulation box (4), a vacuum pump (5), a dynamic gas distribution system (6) and test software (7); the test platform (1) is internally provided with a temperature control device (11), a probe adjusting device (12), a liquid evaporator (13), a fan (14), a lighting unit (15), a vacuum gauge (16), a vacuumizing channel (17), a gas path (18) and a temperature and humidity sensor (19); the temperature control device (11) comprises a sample test bench (111), a built-in heater(112), a temperature sensor (113) and a water cooling protection system (114) wrapping around the heater (112); the temperature control device (11) can directly carry out external temperature control on a test sample; the probe adjusting device (12) comprises an adjusting bracket (121) and an elastic probe (122); and a contact point of the elastic probe (122) and the test sample can be adjusted through the adjusting bracket (121).

The present invention provides methods for predicting response to a hormone-directed therapy or chemotherapy in a prostate cancer (PCa) patient comprising (a) performing a direct analysis comprising immunofluorescent staining and morphological characterization of nucleated cells in a blood sample obtained from the patient to identify and enumerate circulating tumor cells (CTC); (b) individually characterizing genotypic, morphometric and protein expression parameters to generate a profile for each of the CTCs, and (c) predicting response to hormone-directed therapy in the prostate cancer PCa patient based on said profile. In some embodiments, the methods comprise repeating steps (a) through (c) at one or more timepoints after initial diagnosis of prostate cancer to sequentially monitor said genotypic, morphometric and protein expression parameters.