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Branched boric acid functionalized monolithic column and preparation method and application thereof

A monolithic column and dendritic technology, applied in the field of functionalized material devices, can solve the problems of inability to use and the binding force does not reach the antibody level, and achieve the effect of improving binding force, strong anti-interference ability and increasing binding capacity.

Active Publication Date: 2015-04-29
SHANGHAI TOBACCO GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the binding force is far from the level of conventional antibodies, and it cannot be used directly under neutral or weakly acidic conditions. Therefore, it is crucial to further develop boronic acid functionalized materials with higher affinity under neutral or weakly acidic conditions. worthy of in-depth study and discussion

Method used

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  • Branched boric acid functionalized monolithic column and preparation method and application thereof
  • Branched boric acid functionalized monolithic column and preparation method and application thereof
  • Branched boric acid functionalized monolithic column and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Preparation of epoxy-functionalized monolithic column 1

[0075] The epoxy-based functionalized monolithic column is selected as the basic monolithic column. The epoxy-functionalized monolithic column was synthesized by thermally induced free radical polymerization. Before preparing the monolithic column, the capillary was successively treated with acid, alkali activation and γ-MAPS derivatization, and then 30 mg of monomer glycidyl methacrylate (GMA) containing epoxy functional groups, crosslinking agent N, N′ - Methylenebisacrylamide (MBAA) 60mg, porogen 174mg lauryl alcohol and dimethyl sulfoxide 200mg total 374mg mixed with initiator azobisisobutyronitrile (AIBN) 1mg, vortexed for 5 minutes, and then ultrasonicated for 30 Minutes to obtain a uniform pre-polymerization solution. Fill it into a double bond-modified capillary, and put it into a 75°C water bath for 12 hours of reaction. After the polymerization reaction is completed, unreacted substances a...

Embodiment 2

[0076] Example 2: Preparation of epoxy-functionalized monolithic column 2

[0077] The epoxy-based functionalized monolithic column is selected as the basic monolithic column. The epoxy-functionalized monolithic column was synthesized by thermally induced free radical polymerization. Before preparing the monolithic column, the capillary was successively treated with acid, alkali activation and γ-MAPS derivatization, and then 25 mg of monomer glycidyl methacrylate (GMA) containing epoxy functional groups, crosslinking agent N, N′ - Methylenebisacrylamide (MBAA) 55mg, porogen 160mg lauryl alcohol and dimethyl sulfoxide 200mg total 360mg were mixed with initiator azobisisobutyronitrile (AIBN) 1mg, vortexed for 4 minutes, and then ultrasonicated for 25 Minutes to obtain a uniform pre-polymerization solution. Fill it into a double bond-modified capillary, and then put it in a 70°C water bath for 11 hours of reaction. After the polymerization reaction is completed, unreacted subs...

Embodiment 3

[0078] Example 3: Preparation of epoxy-functionalized monolithic column 3

[0079] The epoxy-based functionalized monolithic column is selected as the basic monolithic column. The epoxy-functionalized monolithic column was synthesized by thermally induced free radical polymerization. Before preparing the monolithic column, the capillary was successively treated with acid, alkali activation and γ-MAPS derivatization, and then 35 mg of monomer glycidyl methacrylate (GMA) containing epoxy functional groups, crosslinking agent N, N′ - Methylenebisacrylamide (MBAA) 65mg, porogen 180mg lauryl alcohol and dimethyl sulfoxide 200mg total 380mg were mixed with initiator azobisisobutyronitrile (AIBN) 1mg, vortexed for 6 minutes, and then ultrasonicated for 35 Minutes to obtain a uniform pre-polymerization solution. Fill it into a double bond-modified capillary, and then place it in a water bath at 80°C for 13 hours to react. After the polymerization reaction is completed, unreacted su...

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Abstract

The invention provides a branched boric acid functionalized monolithic column which is a flexible branched boric acid functionalized monolithic column obtained by bonding flexible branched macromolecules onto the hole surface of a base monolithic column through the replacement of boric acid functionalization. The invention further provides a preparation method of the branched boric acid functionalized monolithic column and the application thereof in separation, enrichment and purification of biomolecules containing cis-dyhydroxyl structures. According to the branched boric acid functionalized monolithic column and the preparation method and the application thereof, the monolithic column is high in affinity and capacity, wide in adaptive PH range, high in capacity of resisting disturbance and being suitable for direct analysis of practical samples without PH adjustment.

Description

technical field [0001] The invention belongs to the technical field of functionalized material devices, and relates to a dendritic boric acid functionalized monolithic column and its preparation method and application, in particular to the preparation of a high-affinity, high-capacity flexible dendritic boric acid functionalized monolithic column and its application in complex samples Applications in the separation, enrichment and purification of biomolecules containing cis-dihydroxyl structures (such as low-abundance glycoproteins and carbohydrate compounds, etc.). Background technique [0002] Among the research objects of proteomics, metabolomics and glycomics, a class of biomolecules containing cis-dihydroxyl structures such as glycopeptides and glycoproteins, nucleosides and nucleotides, and monosaccharides and polysaccharides are an important class. of biomolecules. These cis-dihydroxy biomolecules play an extremely important role in physiological processes and early ...

Claims

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Application Information

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IPC IPC(8): B01J20/285B01J20/30B01D15/22G01N1/34G01N1/40
CPCB01D15/22G01N1/34G01N1/40B01J20/285B01J20/3085B01J2220/54
Inventor 刘震李道金张乾刘百战
Owner SHANGHAI TOBACCO GRP CO LTD
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