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451 results about "Sample mass" patented technology

Steroid hormone analysis by mass spectrometry

Methods, systems and kits for the simultaneous or sequential analysis of a multitude of steroid hormones by mass spectrometry are disclosed. The methods require minimal sample size and minimal preparation time. The methods include ionizing the hormones and analyzing the hormones by mass spectrometry. In addition, methods, systems and kits for the simultaneous or sequential analysis of steroid hormones are disclosed including ionization of the steroid hormones by photoionization.
Owner:GEORGETOWN UNIV

Mass spectrometer with a plurality of ionization probes

A multiple sample mass spectrometer having multiple atmospheric pressure ionization probes, each forming an ion spray with passages associated with each of said ion sprays for introducing sample ions into the mass spectrometer for analysis. The flow of ions through selected passages is selectively blocked whereby to permit analysis of ions from selected ionization probes.
Owner:THERMO FINNIGAN

Method and system for sample size determination for database optimizers

A system and method for determining an adequate sample size for statistics collection is disclosed. A mechanism for automatically determining an adequate sample size for both statistics and histograms is provided. The sample size determination is accomplished via an iterative approach where the process starts with a small sample, and for each attribute which may need more data, the sample size is increased while restricting the information collected to only those attributes that require the larger sample.
Owner:ORACLE INT CORP

Methods for mass spectrometry detection and quantification of specific target proteins in complex biological samples

Mass spectrometry-based methods are described for the detection or quantification of targeted proteins in biological samples e.g., plants, animals, and microorganisms, parts (e.g., tissue or cells) thereof, and products derived from plants, animals or microorganisms.
Owner:ISTA

On-site method of providing analysis of potency and purity of pharmaceutical compounds

A computer facilitated method of requesting a spectral analysis of a sample having an unknown concentration or purity, performing an energy absorption analysis of the sample to obtain spectral data regarding the analysis and comparing the spectral data to a stored spectral data regarding the analyses of the samples having a predetermined concentration and purity to determine the concentration or purity of the sample having an unknown concentration or purity. The spectral analysis is performed on site where the sample is prepared or administered using a portable analytical apparatus and provides a real time report of the concentration or purity of the sample. The apparatus requires only a small sample size.
Owner:DYNALABS LLC

Methods For Preparing Sequencing Libraries

Improvements in chromatin immunoprecipitation-high throughput sequencing techniques has allowed the creation of chromatin maps from limited biological sample sizes that cannot be evaluated using conventional chromatin immunoprecipitation-sequencing protocols. For example, a modified universal primer is utilized that incorporates restriction enzymes into chromatin immunoprecipitation fragments before amplification. The improved method allows the sample sizes to be several orders of magnitude less than that required for standard ChIP-Seq techniques.
Owner:THE GENERAL HOSPITAL CORP +1

Micro-gyroscope for detecting motions

The invention relates to a micro-gyroscope for detecting motions relative to an X and / or Y and Z axis, particularly as a 3D, 5D, or 6D sensor. Sample masses are disposed uniformly about an anchor and can be driven radially relative to the central anchor. Anchor springs are disposed to attach the sample masses to a substrate, and these sample masses can be deflected both radially within and out of the X-Y plane. A sensor mass is disposed on one of the sample masses by means of sensor springs, and the sensor springs allow deflection of the sensor mass within the plane of the sample mass, and orthogonal to the radial drive direction of the sample masses. Drive elements oscillate these sample masses in the X-Y plane, and sensor elements captures the defection of the sample masses due to the Coriolis forces generated when the substrate is rotated.
Owner:HANKING ELECTRONICS LTD

Object detection model training method and target object detection method

The invention relates to a target object detection method and device, a computer readable storage medium and computer equipment. The method comprises the steps that acquiring a to-be-detected image; inputting the to-be-detected image into the target object detection model, wherein the target object detection model is obtained by carrying out parameter adjustment on an initial object detection model through training a loss value; wherein the training loss value is obtained through calculation according to target pixel points determined by a first area and a second area, and the first area and the second area are obtained through determination according to the position of the sample mass center of a target type object in the training sample image; generating a prediction graph correspondingto the to-be-detected image by the target object detection model, wherein the prediction graph describes the relation degree of each pixel point of the to-be-detected image belonging to the target detection object; and carrying out region segmentation on the prediction image to obtain a target detection object region. In addition, the invention further provides an object detection model training method and device, a computer readable storage medium and computer equipment.
Owner:HUAZHONG UNIV OF SCI & TECH +1

Product service life rapid test method based on physical model

A product service life rapid test method based on a physical model comprises the steps of determining an equivalent environment profile; determining acceleration test conditions; determining an upper quality specification limit of the test; and determining a plurality of groups of sampling plans (n, c) under the given reliability and user risk. According to the method, the degradation physical model and acceleration tests are combined, so that forming of service life rapid test schemes of products of different types can be effectively guided, and whether the batch of products can meet service life index requirements under specified utilization conditions and environment conditions is tested within a short time. The stability rapid test method for product performance parameters is provided through the performance parameter physical model, so that the test sample size and test time can be saved, and research and development costs can be reduced; and by means of the acceleration test method, product batch production test time can be greatly shortened, and test procedures can be simplified.
Owner:BEIHANG UNIV

In-vitro cell micronuclei method for detecting total particulate matters of main stream smoke of cigarettes

The invention discloses an in-vitro cell micronuclei method for detecting total particulate matters of main stream smoke of cigarettes. The method comprises the steps of seeding cells on a 96-hole plate for cigarette smoke contamination, directly fixing, dyeing, and feeding the cells into a high-connotation imaging system for detection; setting fluorescence intensity parameters of main nucleuses and the micronuclei of the cells according to the difference between a fluorescence value of cell nucleuses and a background fluorescence value, measuring the sizes of the main nucleuses and the micronuclei of the cells so as to set nucleus diameter parameters, and setting distance parameters according to distances between the cell nucleuses; performing analysis on images according to the setting, and selecting the number of binucleated cells with the micronuclei and the total number of the binucleated cells from a result lead-out column so as to calculate the micronucleus rate. According to the method, the number of required cells is small, the cells are not need to be used, and micronuclei sheets are not required to be manufactured; furthermore, the in-vitro cell micronuclei method is high in detection speed, large in sampling quantity and high in accuracy.
Owner:CHINA TOBACCO YUNNAN IND

Cast membrane structures for sample preparation

A method for casting-in-place composite and / or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind / elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.
Owner:MILLIPORE CORP

High density cast-in-place sample preparation card

A card or insert having a plurality of recesses for a sample preparation device, the card containing cast-in-place composite and / or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular card size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a plurality of recesses in an insert for a multi-well sample preparation device, thereby providing an effective platform for high throughput micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted in multiplicity, including selective bind / elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. Manufacturing flexibility and high throughput is achieved. The card can be configured for direct analysis of bound sample without elution.
Owner:MILLIPORE CORP

Micro-fluidic chip and research method for capturing and detecting exosome

The invention provides a micro-fluidic chip and a method for capturing and detecting an exosome. The micro-fluidic chip is composed of a glass substrate and a PDMS membrane. The PDMS membrane is provided with two curved mixing channels, two collection tanks, five collection channels, four sample inlets and a liquid outlet. Through combination of the micro-fluidic chip and immunomagnetic nanoparticles, a trace amount of an exosome in a liquid can be captured. Through the micro-fluidic chip, the exosome capture and detection can be finished on one technology platform. The method has the advantages of small sample amount and short detection time.
Owner:DALIAN MEDICAL UNIVERSITY

Determining confidence intervals for weighted trial data

Collected trial data is weighted to, for example, reflect the relevance of the data. A weighted confidence interval determination and application process described herein determines a confidence interval used to interpret the data. The confidence interval is derived by adjusting the sample size N to account for the impact of weighting when determining confidence intervals. The sample size N is adjusted in a downward trend to avoid overestimating the confidence interval. Lower and upper bounds of the confidence interval are determined using weight influenced variables. Thus, interpretation of weighted trial data, such as product demand data, can be achieved and acted upon with an accurate estimation of risk.
Owner:VERSATA DEV GROUP

Method for detecting fetal thalassemia pathogenic gene and kit

The invention discloses a method for detecting a fetal thalassemia pathogenic gene, which comprises the following steps of: (1) screening SNP sites, wherein the SNP sites are used for designing a primer pool of the thalassemia gene in an amplification genome and for capturing the probe of the thalassemia gene of the free DNA in the plasma of a pregnant woman; (2) extracting the free DNA in the plasma of the pregnant woman and the whole blood genomic DNA of the father, the mother and a born sibling and constructing a corresponding DNA library, and carrying out template preparation and enrichment; (3) sequencing the free DNA and the whole blood genomic DNA library in step (2); (4) constructing the haploid genotype of the SNP sites on the thalassemia gene, combining the sequencing informationof the free DNA and the whole blood genomic DNA library, analyzing the genetic condition of the parent source and the genetic condition of the parent source, so as to determine the corresponding genotype of the SNP sites of the fetal. According to the method, target area capture and high-throughput sequencing technology are used, so that the noninvasive antepartum detection of the thalassemia isrealized; the required sample amount is small; on the basis of detecting the mutation of the parent source, the method can realize the detection of the gene mutation of the maternal source of the fetal.
Owner:GUANGZHOU DARUI BIOTECH +1

Similar product information-based bayesian reliability evaluation method of small number samples

The invention relates to a similar product information-based bayesian reliability evaluation method of small number samples. The method comprises the steps of (S1) carrying out similarity scoring on similar products and determining a prior sample set; (S2) acquiring prior information, which includes failure life data and pseudo failure life data of the similar products; (S3) determining a prior distribution form; (S4) checking the failure mechanism consistency of the similar products; and (S5) determining prior distribution and posterior distribution and achieving bayesian reliability evaluation. According to the method provided by the invention, the sample quantity of the small number samples is effectively improved; and the prior information includes the failure life data and the pseudo failure life data and a two-parameter bayesian reliability evaluation problem is converted into single-parameter bayesian reliability evaluation, so that the bayesian reliability evaluation accuracy of the products is effectively improved and the calculation method of bayesian reliability parameter evaluation is simplified.
Owner:SHANGHAI RADIO EQUIP RES INST

Method for detecting content of 3-monochloropropanol-1, 2-diol ester in edible oil

The invention relates to a method for detecting content of 3-monochloropropanol-1, 2-diol (3-MCPD) ester in edible oil. The method comprises the following steps: preprocessing a sample, carrying out acid-catalytic ester exchange, salting out, extracting, purifying, carrying out derivatization and detecting. According to the method, the internal labeling of stable isotopes is adopted, and the acid-catalytic ester exchanging is combined with the derivatization of heptafluorobutyrylimidazole to indirectly quantitatively analyze 3-MCPD ester by utilizing a single four-level lever GC-MS, so that the preprocessing time is shortened, the analysis efficiency is improved, the consumption amount of an organic solvent is greatly reduced, the environmental pollution is avoided, meanwhile, the analysis cost is also reduced, after the derivatization of heptafluorobutyrylimidazole, the information of a sample mass spectrum fragment is rich, and a gas chromatograph-mass spectrometer is hardly polluted.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI

High-throughput sequencing-based detection kit for SNP loci and detection method for detection kit

The invention relates to a high-throughput sequencing-based detection kit for SNP loci used for detecting 273 SNP loci (234 SNP loci are located on an autosome, 9 SNP loci are located on a Y chromosome and 30 SNP loci are located on an X chromosome). The detection kit comprises a primer composition formed by primer pairs of 273 SNP loci. The sequence of the primer composition is as shown in SEQ ID NO:1 to SEQ ID NO:546. The invention further provides a detection method using the kit, and an application of the kit in the field of judicial identification of triplet paternity test, diad paternity test, grandparent and grandchild test, siblings identification and individual recognition. Parallel sequencing is carried out on the SNP loci by adopting a high-throughput sequencing technology, and variation information of a flanking sequence can be obtained while locus information is read; sequence information of 384 samples on the 273 SNP loci can be obtained through single sequencing, so that the DNA sample capacity is reduced and the detection time is shortened; and fragment libraries are concentrated below 200bp and are suitable for forensic degradable materials.
Owner:ACADEMY OF FORENSIC SCIENCE

Sequential sampling within a portable computing environment

A sequential sampling tool that executes advanced sequential protocols on a portable platform. The system includes a computer and software code compiled with well-known statistical and graphical user interface packages. The sampling and testing tool supports SPRT, 2-SPRT, and sequential Bayes sampling protocols for a variety of statistical distributions, including Bernoulli, binomial, Poisson, negative binomial, and normal distributions. The user has easy access to properties of the statistical design, such as average sample size curve, the operating characteristic curve, and the Type-1 and Type-2 error rates. The system also displays two boundary lines for the design, and allows the user to plot and visualize each observation point one-at-a-time as each sample unit is taken and counted.
Owner:JESKE DANIEL R +5

Coal rock chitin group microscopic image classification method and system based on transfer learning

The invention discloses a coal rock chitin group microscopic image classification method and system based on transfer learning, and belongs to the technical field of image processing. The method comprises the following steps: S1, collecting samples and expanding the number of samples; S2, obtaining a pre-trained model; S3, constructing a coal rock chitin group microscopic component identificationmodel; and S4: carrying out component identification. In the step S1, the data enhancement process is as follows: S11, dividing the obtained samples into a training set and a test set; and S12, performing random scaling, random horizontal translation and vertical translation on the images in the training set to realize data expansion. On the basis of the transfer learning method, a target data setis trained by sharing the parameters of a convolution layer and a pooling layer in the pre-trained network, so that a model with good generalization ability can be trained under the condition that the sample size of the chitin group is limited, effective classification of the coal rock chitin images is realized, and the method and the system are worthy of popularization and application.
Owner:ANHUI UNIVERSITY OF TECHNOLOGY

Industrial-grade intelligent surface defect detection method

The invention relates to an industrial-grade intelligent surface defect detection method, which comprises the following steps: constructing and training to generate a twin generative adversarial network GAN, repairing an input image into a normal sample through an improved GAN network, and comparing the output with a manually labeled positive sample through a twin CNN network to obtain a difference, which is a defect. The twin generative adversarial network designed by the invention does not need a large number of samples and does not need to perform data amplification, can solve the problem of small sample size of common industrial products, and reduces the occurrence of an overfitting phenomenon caused by the small number of samples and zero samples in deep learning, so that the defect detection during the development of products and new products with small defect sample size becomes possible. A cross alignment loss function CA and a distribution alignment loss function DA are used to enhance the relationship between two network outputs, and a good classification and identification effect is obtained. The model training speed is improved through an Attention mechanism and a hardware GPU, so that industrial rapid deployment becomes possible.
Owner:BEIJING ZODNGOC AUTOMATIC TECH

Digital microfluidic chip and pathogen immunoassay method based thereon

The invention relates to the technical field of microfluidic chips, in particular to a digital microfluidic chip and a pathogen immunoassay method based thereon. The method comprises the following steps of: (1) making a digital microfluidic chip; (2) modifying the antigen in a detection region of the digital microfluidic chip; and (3) adding the sample and running the digital microfluidic chip, photographing and detecting a fluorescent signal through a fluorescence detection system CCD and performing a quantitative analysis. The digital microfluidic chip comprises a sample hole, a reagent electrode forming a reagent reservoir, a transport electrode forming a droplet operation channel, and a detection electrode forming a detection region. The digital microfluidic chip and the pathogen immunoassay method based thereon have short reaction time, high detection sensitivity, simple peripheral equipment, high flexibility and low energy consumption, can meet the needs of community hospitals, remote clinics and the like, are suitable for building portable analytics platforms, can be analyzed in batches or in a single sample, and has strong adaptability to the sample size.
Owner:DALIAN UNIV

Method for detecting nitrite in blood

The invention discloses a method for rapidly detecting nitrite in blood. A porous plate and a microplate reader are used to measure the absorbancy of a colored complex formed by the nitrite and Griess, and the blood containing no nitrite is taken as a matrix solution for making a working curve, so that the interference of a blood matrix is eliminated, and the sensitivity and the accuracy of the detection are improved. The method has the characteristics of high accuracy, large detection sample capacity, small detected sample amount, high detection efficiency and the like.
Owner:云南健牛环境监测有限公司

Sample size extraction determination method for testability verification of airplane airborne equipment

The invention provides a sample size extraction determination method for testability verification of airplane airborne equipment. The sample size extraction determination method includes the three links of preliminary sample size determination, sample size distribution and sample size supplement. The preliminary sample size of various levels is eventually determined according to testability index parameters of different levels and added four constrained parameters of n1, n2, n3 and n4. According to the particular cases of products of various levels of the airplane airborne equipment, the upper limit is set to be 1000 for the preliminarily determined sample size, and the defects that that test workloads are too large, and the test cycle is too long are overcome. When samples are distributed, the constraint condition of calculating the minimum n (n is a positive integer) in the formula of Cpimin * 10n> / = 1 is designed, the accumulation range is provided for sample size supplement, and the samples can be conveniently distributed. Eventually, the method for supplementing samples is provided, the failure occurrence probability and failure distribution are considered, and an implementation means for test verification is considered.
Owner:BEIJING AEROSPACE MEASUREMENT & CONTROL TECH

On-site method of providing analysis of potency and purity of pharmaceutical compounds

A computer facilitated method of requesting a spectral analysis of a sample having an unknown concentration or purity, performing an energy absorption analysis of the sample to obtain spectral data regarding the analysis and comparing the spectral data to a stored spectral data regarding the analyses of the samples having a predetermined concentration and purity to determine the concentration or purity of the sample having an unknown concentration or purity. The spectral analysis is performed on site where the sample is prepared or administered using a portable analytical apparatus and provides a real time report of the concentration or purity of the sample. The apparatus requires only a small sample size.
Owner:DYNALABS LLC

High-confidence small-sample evaluation method for reliability of explosives

The invention discloses a high-confidence small-sample evaluation method for the reliability of explosives. According to the mensurability and reliability requirements of explosives, and under the premise that the performance parameters of explosives obey normal distribution or approximate normal distribution or the evaluated performance parameters after transformation obey normal distribution or approximate normal distribution, the parameters and uncertainty of a population distribution model are estimated based on small sample data and measurement uncertainty of product performance parameter quantitative measurement. In the absence of priori information and in the presence of statistical characteristics, the number of pieces of sample data needed by the method can be reduced to five under the condition that the confidence level is not less than 0.90 and the reliability of explosive performance evaluation is not less than 0.99 or above. The confidence level of evaluation results reaching required reliability is not lower than 97.5% of the prescribed confidence level.
Owner:INST OF CHEM MATERIAL CHINA ACADEMY OF ENG PHYSICS

Sample analyzing method and sample analyzing device

This invention relates to a technique for analyzing a sample. A sample analyzer (1) provided by the invention includes: a voltage applier (12) for applying a voltage to a reaction field which includes a sample; a response measurer (13) for measurement of a response to the voltage applied to the reaction field; a selector (11) for selecting a first voltage application state for measurement of a first response for use in calculation necessary for analyzing the sample or a second voltage application state for measurement of a second response for use in determining whether or not the reaction field has been supplied with a target amount of the sample; an arithmetic operator (17) for calculation necessary for analyzing the sample based on the first response; a determiner (17) for determination, based on the second response, on whether or not the reaction field has been supplied with the target amount of sample; and a controller (15) which makes the selector select the second voltage application state after making the selector select the first voltage application state.
Owner:ARKRAY INC

Layer-by-layer analysis method for surface layer of copper alloy material

ActiveCN102539517ARealize Layer-by-Layer AnalysisLayer-to-layer resolution improvementMaterial analysis by electric/magnetic meansMass spectrometrySpectrometer
The invention provides a layer-by-layer analysis method for a surface layer of a copper alloy material, comprising the steps of: selecting a glow discharge ion source and a mass spectrum analysis condition to carry out sample treatment and sample analysis; peeling an analyzed sample by utilizing the glow discharge ion source under the action of cathode sputtering; recording a continuous change signal of analyzed sample mass spectrum peak strength along with the time in a high-sensitivity mass spectrometer system in real time; and converting the continuous change signal into change of the chemical composition of the sample along with the depth by using a composition and depth double-calibration method so as to finish the layer-by-layer quantified analysis of the surface layer of an aluminum bronze membrane sample on a brass base material. In the invention, proper analysis parameters such as argon pressure, discharge voltage, sample sputtering spot surface diameter, sample measurement time and the like are selected to improve interlayer resolution to be thousands of nanometers, so that the ultrathin layer-by-layer analysis of the surface layer of bronze on the brass base material is realized.
Owner:GUOBIAO BEIJING TESTING & CERTIFICATION CO LTD

Method and system for detecting residual poison in human body

A method and system for detecting residual poison in human body are provided. Using the disclosed HPLC-Chip-mass spectrometry (MS) / MS and / or HPLC-MS / MS method to detect the residual poison, the method of the present invention mainly includes sample preparation, liquid chromatography and mass spectrometry. The method of the present invention has advantages of low sample size, high specificity, low detection limit, high sensitivity, low cost, high accuracy and stability, etc.
Owner:THE HONG KONG UNIV OF SCI & TECH

Programmable, digital vacuum-operated liquid sampler

A programmable liquid sampler for obtaining and digitally analyzing multiple, sequential samples in real time from an external source of liquid to be analyzed comprises an adaptive feedback algorithm for correcting sample weight measurements occasioned by vacuum delays. A sampling assembly receives and at least temporarily stores a sample drawn by suction through an air port that can also pressurize the chamber. A load cell determines sample mass through a logic controller that implements adaptive feedback by recognizing a desired target sample size, requesting an initial sample, and measuring the initial sample size. A variable scaling factor is derived by comparing the target sample size to said measured sample size and the subsequent requesting size is scaled. Samples can be stored in multiple compartments of a radial table that is indexed by the computer software for proper sample control.
Owner:TELEDYNE INSTR INC
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