63 results about "Micronucleus" patented technology

The invention discloses an in-vitro cell micronuclei method for detecting total particulate matters of main stream smoke of cigarettes. The method comprises the steps of seeding cells on a 96-hole plate for cigarette smoke contamination, directly fixing, dyeing, and feeding the cells into a high-connotation imaging system for detection; setting fluorescence intensity parameters of main nucleuses and the micronuclei of the cells according to the difference between a fluorescence value of cell nucleuses and a background fluorescence value, measuring the sizes of the main nucleuses and the micronuclei of the cells so as to set nucleus diameter parameters, and setting distance parameters according to distances between the cell nucleuses; performing analysis on images according to the setting, and selecting the number of binucleated cells with the micronuclei and the total number of the binucleated cells from a result lead-out column so as to calculate the micronucleus rate. According to the method, the number of required cells is small, the cells are not need to be used, and micronuclei sheets are not required to be manufactured; furthermore, the in-vitro cell micronuclei method is high in detection speed, large in sampling quantity and high in accuracy.

The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.

The invention provides a method for detecting influence of a buccal tobacco product on micronucleus rates of cells. The method comprises the following steps of performing a sample pretreatment method, performing preparation of single-cell suspension, calculating cell concentration, performing cell inoculation, adding a test object, incubating the test object, harvesting the cells, performing hypotension, dropping to a slider, dyeing, performing micronucleus calculation, performing result determination and the like. According to the method provided by the invention, by comprehensively surveying the exposure way and the action mode of the buccal tobacco product, the sample pretreatment method for the buccal tobacco product is established; moreover, human oral mucosal fibroblasts hOMF are selected according to action target cells of the buccal tobacco product to serve as cells for in vitro micronucleus test detection on the buccal tobacco product, and the sample detection dosage is determined, and therefore an in vitro micronucleus test detection method suitable for the buccal tobacco product is formed.

The invention is a method for analyzing immature reticulocytes for the presence of micronuclei. The method includes reticulocyte enrichment, fluorescent labeling, micronuclei staining, and analysis using single-laser flow cytometry. The invention also includes kits containing reagents to use in the method.

A cigarette mainstream smoke genotoxicity measurement method is characterized in that: the cultured cells are directly placed in cigarette mainstream smoke diluted by pure air to expose, the exposed cells are cultured, centrifuged and fixed by cold methanol, acridine orange solution is used for the fluorescent staining for the cells, the stained cells are observed in low power lens to select the region which is distributed evenly and stained well and calculate the number of cells with microkernel(including mononuclear, binuclear and multinuclear) of 500 cells in each sample under fluorescent microscope, the samples is compared with the control sample exposed in pure air to judge whether the cigarette mainstream smoke can increase the cell micronucleus and judge the genotoxicity of the cigarette mainstream smoke. The genotoxicity of the cigarette mainstream smoke can be quantitative evaluated by the invention and the invention has simple operation, high sensitivity and reliable result, thus establishing a new method for measuring cigarette mainstream smoke genotoxicity.

The present invention is directed to novel assays to measure the genotoxic effects of compounds on erythroid cells in vitro.

The invention discloses a peripheral blood lymphocyte micronucleus detection kit and a detection method thereof. The detection kit provided by the invention comprises erythrocyte hemolysin, a red fluorescent dye solution, a lysis solution and a positive standard product, wherein the lysis solution comprises sodium chloride, sodium citrate, Triton X-114, green fluorescent dye and RNase; and a peripheral blood lymphocyte micronucleus detection method based on the kit and a flow cytometry detection method is provided. An experiment proves that the peripheral blood lymphocyte micronucleus detection kit and the peripheral blood lymphocyte micronucleus detection method are quick and accurate; on the premise of guaranteeing objective and true experimental results, the detection cost is reduced, and the experiment has repeatability and can be widely applied to clinical diagnosis and monitoring of occupational exposure genetic damage of healthy people, rehabilitation detection of genetic damage of cancer patient and cellular genetic science research.

The invention provides an anti-fatigue healthcare product or pharmaceutical composition and a preparation method and application thereof. The healthcare product or the pharmaceutical composition is prepared from, by weight, 50-90 parts of acanthopanax root, 30-50 parts of glossy ganoderma, 30-50 parts of wolfberry fruit and 60-110 parts of green tea. The healthcare product or the pharmaceutical composition is capable of effectively reducing organism serum urea nitrogen and blood lactic acid content and obviously prolonging load swimming time and has a good anti-fatigue effect. Besides, the healthcare product or the pharmaceutical composition is capable of increasing radiated mouse SOD (superoxide dismutase) activity, increasing white blood cells, lowering marrow micronucleus rate and increasing animal survival rate, has a remarkable anti-radiation function and is safe, non-toxic, good in taste and suitable for long-term taking.

The invention discloses a method for detecting the influence of an electronic cigarette product on the micronucleus rate of cells. The method comprises the following steps: preprocessing test samples, preparing a human lung fibroblast single cell suspension fluid, calculating the concentration of the human lung fibroblast single cell suspension fluid, inoculating the human lung fibroblast single cell suspension fluid, dividing the test samples into groups, setting the detection dosages of the test samples, preparing a test sample culture medium, subjecting the test sample culture medium to low permeability, and preparing a droplet slice of the test sample culture medium. The using characteristics and effect modes of electronic cigarette products are taken into account, an electronic cigarette product sample preprocessing method is established; according to the electronic cigarette product acted target cells, human lung fibroblast is taken as the cell for the cytotoxicity tests of electronic cigarette products, the sample detection dosage is determined at the same time, and thus an in-vitro micronucleus test method suitable for the electronic cigarette products is established.

The invention provides a method for detecting the genetic toxicity of organic pollutants. The method comprises the following steps of: (1) treating gobiocypris rarus by a water solution of an organic-pollutant-containing sample to be detected in an exposed way; and (2) detecting the peripheral blood erythrocyte of the gobiocypris rarus which is treated in the exposed way, carrying out statistics on cell micronucleus rate and drawing a dosage-effect curve. The detection method is sensitive, simple, convenient, rapid, more visual and accurate, has good result repeatability, and has important significance for the research of genetic toxicity of water quality.

The invention provides an artificial intelligence identification method for a peripheral hemolymph micronucleus cell image, and the method comprises the steps: collecting a to-be-analyzed sample through a collection device, and obtaining a cell image; carrying out segmentation processing on the obtained cell image to obtain a foreground to-be-detected area; identifying, classifying and counting the cells on the foreground to-be-detected area; storing the cell type information on the foreground to-be-detected area and the position information of the foreground to-be-detected area on the cell image to form traceable information; and forming an image-text report according to the traceable information, and storing the image-text report as an artificial intelligence system learning training material. According to the artificial intelligence identification method for the peripheral hemolymph micronucleus cell image provided by the invention, automatic identification and classification of specific images of lymphoblasts, micronucleus cells and nude nuclei of peripheral blood can be realized. Compared with an existing microscope artificial microscopic examination method, the statistical accuracy is higher, and the detection speed is remarkably increased.

The invention relates to a cigarette smoke in-vitro micronucleus detection method. The cigarette smoke in-vitro micronucleus detection method is characterized in that Chinese hamster ovary cells are selected to carry out the micronucleus test, and the micronucleus frequency is detected by cell culture, preparation of cigarette smoke condensate, cell contamination, fixing and dyeing. Compared withthe prior art, the cigarette smoke in-vitro micronucleus detection method has the advantages that in-vitro micronucleus test is adopted to evaluate the influence of cigarette smoke on the cell chromosomal aberration, not only the experiment cost can be reduced, the test time can be shortened and richer experiment result can be obtained, but also the in-vitro micronucleus test can obtain the resultconsistent to the in-vivo micronucleus test, as well as equivalent sensibility, specificity and accuracy; besides, the source of the micronucleus can be analyzed on basis of the obtaining of the cellmicronucleus frequency.

The invention discloses a method for detecting water quality genetic toxicity by a broad bean root tip micronucleus test, comprising the following steps of: soaking seeds for Songrun green-peel beans, accelerating germination, and obtaining a seed of which the primary root has the length of 1.5-2cm; respectively processing seed root tips by Cr6+ solutions of different concentrations; repairing, fixing and dyeing root tip cells; carrying out microscopic examination to the root tips, and calculating the permillage of cell micronucleus processed by the Cr6+ solutions of different concentrations; drawing the standard curve for the permillage of cell micronucleus relative to Cr6+ concentration; then, measuring the permillage of cell micronucleus of a water sample to be detected with the same method; and substituting the standard curve to obtain the water quality toxicity of the water sample to be detected, which is represented by Cr6+ concentration. In the method, Cr6+ is selected as the standard toxic substance, and the quantitative evaluation of the water quality toxicity is realized by the standard curve of the micronucleus permillage relative to Cr+6. The genetic toxicity of the water quality can be more intuitively and accurately detected by the method, results are easy to unify, and the repeatability is good.

The invention discloses a method for culturing a double-micronucleus paramecium by inflating a plastic film bag, and belongs to the field of aquiculture. The technical key points are that a paramecium culture solution is prepared, then inflation culture is conducted in the plastic film bag, and two types of fresh water microalgae-chlamydomonas reinhardii and chlorella pyrenoidosa are complementally fed in the last four days of the culture. Compared with the traditional technology, the innovativeness is that the paramecium is cultured by adopting the plastic film bag, and the paramecium is quick in growth, strong in somatoplasm and high in yield; the plastic film bag is good in light-permeating property, can be repeatedly used, and has low possibility of being polluted, the operation is convenient, and fund can be saved.

As described herein, chromosomal missegregations, chromosomal micronuclei, cytosolic DNA, and combinations thereof are indicative of metastatic cancer. Methods and compositions are described herein that are useful for detection and treatment of patients with chromosomal instabilities such as chromosomal missegregations, chromosomal micronuclei, cytosolic DNA, and combinations thereof. For example,some of the methods and compositions include use of kinesin-13 proteins such as Kif2b, MCAK/Kif2c, or KIF13A. The methods and compositions can also include inhibitors of STING, ENPP1, cGAS, NF-kB transcription factor p52, NF-kB transcription factor RelB, or any combination thereof. Methods are also described for identifying compounds that are effective for treatment of cancer, including metastatic cancer.

The invention discloses a method for preparing a brown ginseng extract with high antitumor activity and an application of the method. The whole process is characterized in that the brown ginseng extract with high antitumor activity is quickly and conveniently prepared finally by the steps of enzymatic hydrolysis, high-temperature high-pressure heating, crushing, extraction and the like. Compared with untreated ginseng extracts, the activity of the extract obtained is increased by about 80 times. Cytotoxicity test and animal model experiment results show that compared with untreated common ginsengs, the brown ginseng extract has relatively high anti-tumor and combined anti-tumor activity, has an obvious inhibiting effect on formation of cyclophosphamide-induced bone marrow micronucleus of tumor-bearing mice and has a remarkable improving effect on immunosuppressive action induced by cyclophosphamide, which shows that the brown ginseng extract combined with cyclophosphamide in use can inhibit the toxic and side effects of cyclophosphamide and promote clinical application of cyclophosphamide.

The invention discloses an in-vitro micronucleus detection method for an electronic cigarette aerosol aqueous extract. The method includes the steps of sample pretreatment, single cell suspension preparation, cell concentration calculation, cell inoculation, sample addition and test substance grouping, tested substance incubation, cell harvesting, low permeation, slide dropping, staining, micronucleus observation, result judgment and the like. Use characteristics and an action mode of electronic cigarette products are comprehensively investigated, a brand-new sample pretreatment method is established, human pulmonary fibroblasts HPF are selected as cells for detection, and the sample detection dosage is determined. With the effective treatment of the sample, the optimal setting of the detection dosage and the correct selection of target cells, the method can accurately and effectively detect the influence degree of the electronic cigarette aerosol aqueous extract on cell micronucleus rates, and then the genotoxicity of the electronic cigarette products is determined.

The invention provides a preparation method of a quick-dry scratch-resistant water-borne wood coating. The selectivity of different top and bottom coating film layers is improved by a calcium sulfate whisker and particle filler tonsure combined application technique, so the scratch-resistant property of a single-component water-borne polyurethane wood coating is improved, and the drying time of the coating film is accelerated. The preparation method comprises the following steps of performing hydrothermal reaction on calcium ions, sulfate radicals, CTAB (cetyl trimethyl ammonium bromide) and water; centrifuging at high speed to obtain whiskers with smaller long diameter as a bottom coating filler; centrifuging to obtain a supernatant as a base liquid, using a whisker micronucleus as an inducing crystal, supplementing the sulfate ions, and performing secondary hydrothermal reaction to obtain whiskers with larger long diameter as a top coating filler, wherein the hardness of the obtained coating film is 2H. The preparation method has the advantages that the defects of poor scratch-resistant property and low drying speed of the single-component water-borne wood paint are overcome, and a new technology is developed for the water-borne wood paint with low cost.

The invention provides a technology for detecting TK6 cell chromosome damage genetic toxicity caused by environmental water and an application of the technology and belongs to the fields of environmental science and health risk assessment. The technology for detecting the TK6 cell chromosome damage genetic toxicity caused by the environmental water comprises a pre-treatment process on an environmental water sample, a cell culture and contamination method, a micronucleus slice preparation process and a result analysis method. The provided micronucleus experiment method can be directly applied to monitoring of the genetic toxicity of the environmental water, and important genetic toxicity effect information is provided for water pollution health risk management.

The invention discloses a method for detecting genetic toxicity by using 3D hepatocyte in-vitro micronucleus cells. The method comprises the following steps of (1) constructing a 3D hepatocyte model in vitro by using common humanized hepatocytes HepG2 as a cell material by adopting a scaffold-free culture method combining hanging drops and ultra-low adsorption culture; and (2) carrying out micronucleus cell detection on a compound by using the 3D hepatocyte model, and analyzing the genetic toxicity mechanism of the compound. The in-vitro detection method can be used for analyzing various genetic toxicity mechanisms of the compound, improving the capability of predicting the genetic toxicity of drugs by an in-vitro cell model and providing information related to human body tests.